Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,466,291 (T)6→7 coding (58/921 nt) b2351 → putative glycan biosynthesis enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,466,2851.T100.0% 919.8 / NA 242I18F (ATT→TTT) b2351putative glycan biosynthesis enzyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (122/120);  total (122/120)

Only 100 of 273 total aligned reads displayed.

GGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTA  >  NC_000913/2466193‑2466372
                                                                                             |                                                                                       
ggTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATGTCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCaa                                                                                          <  1:311819/93‑1 (MQ=255)
       tCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑ttttttt                                                                                  >  1:4439284/1‑92 (MQ=255)
        cTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑ttttttta                                                                                 <  1:5106815/93‑3 (MQ=255)
         tATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑tttttttat                                                                                >  2:5926710/1‑90 (MQ=255)
          aTTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑tttttttata                                                                               <  2:3137581/93‑5 (MQ=255)
          aTTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑tttttttata                                                                               <  2:4720578/93‑5 (MQ=255)
            tCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATaaa                                                                              <  2:1438863/93‑1 (MQ=255)
            tCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATaaa                                                                              <  1:5913123/93‑1 (MQ=255)
             cAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATaaaa                                                                             <  2:2507856/93‑1 (MQ=255)
              aaaGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAAc                                                                            >  1:1029109/1‑93 (MQ=255)
              aaaGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAAc                                                                            >  2:2046472/1‑93 (MQ=255)
               aaGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACg                                                                           <  1:5385448/93‑1 (MQ=255)
                aGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACgg                                                                          <  2:5492755/93‑1 (MQ=255)
                aGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACgg                                                                          <  2:3998621/93‑1 (MQ=255)
                 gTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGt                                                                         <  1:4207624/93‑1 (MQ=255)
                  ttCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTa                                                                        <  2:4695905/93‑1 (MQ=255)
                   tCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTAc                                                                       <  1:5562603/93‑1 (MQ=255)
                   tCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTAc                                                                       <  2:1882026/93‑1 (MQ=255)
                     aTTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGt                                                                     >  2:3564813/1‑93 (MQ=255)
                     aTTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGt                                                                     <  2:3844703/93‑1 (MQ=255)
                      ttGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTg                                                                    >  1:5400124/1‑93 (MQ=255)
                       tGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGa                                                                   <  2:4900213/93‑1 (MQ=255)
                        gTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGaa                                                                  >  2:2688132/1‑93 (MQ=255)
                        gTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGaa                                                                  >  2:504741/1‑93 (MQ=255)
                          cTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAAtt                                                                >  2:5805190/1‑93 (MQ=255)
                          cTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAAtt                                                                >  1:5305946/1‑93 (MQ=255)
                          cTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAAtt                                                                >  1:5170572/1‑93 (MQ=255)
                          cTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAAtt                                                                <  1:2206906/93‑1 (MQ=255)
                             tAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCga                                                             <  1:855100/93‑1 (MQ=255)
                              aGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCgaa                                                            <  2:5757430/93‑1 (MQ=255)
                              aGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCgaa                                                            <  1:2337184/93‑1 (MQ=255)
                               gggATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCgaag                                                           >  1:3353702/1‑93 (MQ=255)
                               gggATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCgaag                                                           >  2:3006725/1‑93 (MQ=255)
                                 gATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCgaagaa                                                         <  2:2400567/93‑1 (MQ=255)
                                 gATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCgaagaa                                                         >  2:1688742/1‑93 (MQ=255)
                                    gCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTg                                                      <  2:701900/93‑1 (MQ=255)
                                     cGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGa                                                     <  1:2847387/93‑1 (MQ=255)
                                     cGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGa                                                     <  1:90077/93‑1 (MQ=255)
                                      gAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGaa                                                    >  2:76951/1‑93 (MQ=255)
                                        aaTGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGt                                                  >  2:162417/1‑93 (MQ=255)
                                          tGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCa                                                >  1:3962120/1‑93 (MQ=255)
                                           gAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCat                                               <  2:315556/93‑1 (MQ=255)
                                              gATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATg                                            <  1:1968037/93‑1 (MQ=255)
                                                tatCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGaa                                          >  1:4597627/1‑93 (MQ=255)
                                                  tctcTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGt                                        <  2:371445/93‑1 (MQ=255)
                                                  tctcTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGt                                        >  2:4946479/1‑93 (MQ=255)
                                                  tctcTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGt                                        <  2:2985528/93‑1 (MQ=255)
                                                    tctTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTgg                                      >  1:5963448/1‑93 (MQ=255)
                                                     ctTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGa                                     <  2:1327514/93‑1 (MQ=255)
                                                     ctTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGa                                     <  1:5817152/93‑1 (MQ=255)
                                                      ttGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGaa                                    >  1:1557398/1‑93 (MQ=255)
                                                      ttGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGaa                                    >  2:3090571/1‑93 (MQ=255)
                                                        gTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAAt                                  >  1:244170/1‑93 (MQ=255)
                                                        gTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAAt                                  <  2:5678808/93‑1 (MQ=255)
                                                         tAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATc                                 >  2:1731735/1‑93 (MQ=255)
                                                         tAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATc                                 <  2:1045931/93‑1 (MQ=255)
                                                         tAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATc                                 >  1:3726973/1‑93 (MQ=255)
                                                           gTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGt                               >  1:3787375/1‑93 (MQ=255)
                                                             tCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGttt                             <  1:2867383/93‑1 (MQ=255)
                                                             tCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGttt                             <  1:4685200/93‑1 (MQ=255)
                                                              ccTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGtttt                            <  2:1224354/93‑1 (MQ=255)
                                                              ccTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGtttt                            >  1:4035944/1‑93 (MQ=255)
                                                              ccTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGtttt                            <  1:4468575/93‑1 (MQ=255)
                                                               cTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTc                           >  1:1819335/1‑93 (MQ=255)
                                                               cTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTc                           <  1:2557070/93‑1 (MQ=255)
                                                                tGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCa                          <  1:3528803/93‑1 (MQ=255)
                                                                tGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCa                          >  2:3222741/1‑93 (MQ=255)
                                                                tGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCa                          >  1:3664426/1‑93 (MQ=255)
                                                                tGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCa                          >  1:3023966/1‑93 (MQ=255)
                                                                 gTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCAt                         <  2:234154/93‑1 (MQ=255)
                                                                  tCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATa                        <  2:2836059/93‑1 (MQ=255)
                                                                  tCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATa                        >  1:1433422/1‑93 (MQ=255)
                                                                   cTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATaa                       >  2:4305996/1‑93 (MQ=255)
                                                                    ttCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATaaa                      >  2:2532815/1‑93 (MQ=255)
                                                                    ttCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATaaa                      >  1:128598/1‑93 (MQ=255)
                                                                      taaTGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATg                    <  2:3660897/92‑1 (MQ=255)
                                                                      cAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATg                    <  1:1176983/93‑1 (MQ=255)
                                                                      cAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATg                    <  1:4345895/93‑1 (MQ=255)
                                                                       aaTGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGa                   >  1:2624156/1‑93 (MQ=255)
                                                                        aTGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGAc                  <  2:5314062/93‑1 (MQ=255)
                                                                        aTGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAACTCGAAGAATTTAAGGCATATGAAGTGGAAATCGTTTTCATAAATGAc                  >  1:6174706/1‑93 (MQ=255)
                                                                         tGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACg                 <  2:4555234/93‑1 (MQ=255)
                                                                         tGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACg                 >  2:501505/1‑93 (MQ=255)
                                                                         tGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACg                 >  1:2526017/1‑93 (MQ=255)
                                                                          gaagaagaaGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGCATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACgg                <  1:3382183/93‑1 (MQ=255)
                                                                           aagaagaagCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATTGTTTTCATAAATGACGgc               >  1:3584445/1‑93 (MQ=255)
                                                                           aagaagaagCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGgc               <  2:4842184/93‑1 (MQ=255)
                                                                            agaagaagCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGgca              >  1:5513601/1‑93 (MQ=255)
                                                                                gaagCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCaa          <  2:54359/93‑1 (MQ=255)
                                                                                 aagCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCaaa         <  1:5684454/93‑1 (MQ=255)
                                                                                 aagCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCaaa         <  1:2140656/93‑1 (MQ=255)
                                                                                  agCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAg        <  1:394025/93‑1 (MQ=255)
                                                                                   gCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGa       >  2:2385238/1‑93 (MQ=255)
                                                                                   gCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGa       <  2:2635163/93‑1 (MQ=255)
                                                                                     gATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACg     >  2:5223747/1‑93 (MQ=255)
                                                                                      aTACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGatga    >  2:2210142/1‑90 (MQ=255)
                                                                                      aTACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGc    >  2:4452842/1‑93 (MQ=255)
                                                                                       tACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCt   >  1:4786244/1‑93 (MQ=255)
                                                                                       tACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCt   >  1:5613574/1‑93 (MQ=255)
                                                                                        aCCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTa  >  2:2264185/1‑93 (MQ=255)
                                                                                             |                                                                                       
GGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTA  >  NC_000913/2466193‑2466372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAATCA  >  NC_000913/2466198‑2466384
                                                                                              |                                                                                             
CGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTT                                                                                               >  SRR4302533.6119566/1‑94 (MQ=60)
CGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTT                                                                                               <  SRR4302533.5140220/94‑1 (MQ=60)
 GTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTT                                                                                              >  SRR4302533.4466119/1‑94 (MQ=60)
 GTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTT                                                                                              >  SRR4302533.4481267/1‑94 (MQ=60)
   CTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTtat                                                                                            <  SRR4302533.5137670/94‑4 (MQ=60)
   CTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTtat                                                                                            <  SRR4302533.5957812/94‑4 (MQ=60)
   CTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTtat                                                                                            >  SRR4302533.2110517/1‑91 (MQ=60)
    TATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATA                                                                                            <  SRR4302533.2390613/94‑1 (MQ=60)
    TATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATA                                                                                            <  SRR4302533.5411859/94‑1 (MQ=60)
       TCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAA                                                                                         <  SRR4302533.5948854/94‑1 (MQ=60)
        CAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAAC                                                                                        <  SRR4302533.956719/94‑1 (MQ=60)
        CAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAAC                                                                                        >  SRR4302533.1035155/1‑94 (MQ=60)
        CAAAGTTCATTGCCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAAC                                                                                        <  SRR4302533.2506553/94‑1 (MQ=60)
         AAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACG                                                                                       >  SRR4302533.2834891/1‑94 (MQ=60)
         AAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACG                                                                                       >  SRR4302533.5974858/1‑94 (MQ=60)
         AAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACG                                                                                       >  SRR4302533.1162990/1‑94 (MQ=60)
          AAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGG                                                                                      <  SRR4302533.5417805/94‑1 (MQ=60)
          AAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGG                                                                                      <  SRR4302533.4329509/94‑1 (MQ=60)
            GTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTA                                                                                    <  SRR4302533.4233079/94‑1 (MQ=60)
             TTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTAC                                                                                   >  SRR4302533.5445610/1‑94 (MQ=60)
             TTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTAC                                                                                   >  SRR4302533.5802593/1‑94 (MQ=60)
              TCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACG                                                                                  <  SRR4302533.5596225/94‑1 (MQ=60)
              TCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACG                                                                                  >  SRR4302533.2634377/1‑94 (MQ=60)
               CATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGT                                                                                 <  SRR4302533.2464415/94‑1 (MQ=60)
                ATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTG                                                                                >  SRR4302533.5432572/1‑94 (MQ=60)
                ATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTG                                                                                >  SRR4302533.4054816/1‑94 (MQ=60)
                ATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTG                                                                                <  SRR4302533.733072/94‑1 (MQ=60)
                 TTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGAACGTGA                                                                               >  SRR4302533.4437682/1‑94 (MQ=60)
                  TGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAA                                                                              >  SRR4302533.4098502/1‑94 (MQ=60)
                   GTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAAT                                                                             >  SRR4302533.1387927/1‑94 (MQ=60)
                    TCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATT                                                                            >  SRR4302533.5003133/1‑94 (MQ=60)
                    TCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATT                                                                            >  SRR4302533.5201776/1‑94 (MQ=60)
                    TCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATT                                                                            >  SRR4302533.5337744/1‑94 (MQ=60)
                     CTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTC                                                                           <  SRR4302533.2220514/94‑1 (MQ=60)
                        TAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAA                                                                        <  SRR4302533.860112/94‑1 (MQ=60)
                        TAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAA                                                                        >  SRR4302533.4195932/1‑94 (MQ=60)
                         AGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAG                                                                       <  SRR4302533.867641/94‑1 (MQ=60)
                         AGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAG                                                                       >  SRR4302533.3525036/1‑94 (MQ=60)
                         AGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAG                                                                       >  SRR4302533.3374424/1‑94 (MQ=60)
                         AGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAG                                                                       <  SRR4302533.3126933/94‑1 (MQ=60)
                         AGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAG                                                                       <  SRR4302533.2351650/94‑1 (MQ=60)
                          GGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGA                                                                      <  SRR4302533.2416176/94‑1 (MQ=60)
                          GGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGA                                                                      <  SRR4302533.4707317/94‑1 (MQ=60)
                           GGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAA                                                                     >  SRR4302533.1789493/1‑94 (MQ=60)
                           GGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAA                                                                     >  SRR4302533.2603422/1‑94 (MQ=60)
                            GATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAAT                                                                    <  SRR4302533.4632084/94‑1 (MQ=60)
                              TGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTG                                                                  >  SRR4302533.5592796/1‑94 (MQ=60)
                                CGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAA                                                                <  SRR4302533.2864909/94‑1 (MQ=60)
                                CGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAA                                                                <  SRR4302533.90648/94‑1 (MQ=60)
                                 GAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAG                                                               <  SRR4302533.1555810/94‑1 (MQ=60)
                                 GAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAG                                                               <  SRR4302533.6205605/94‑1 (MQ=60)
                                 GAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAG                                                               >  SRR4302533.4806603/1‑94 (MQ=60)
                                   AATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTC                                                             >  SRR4302533.3052680/1‑94 (MQ=60)
                                    ATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCA                                                            >  SRR4302533.3986259/1‑94 (MQ=60)
                                     TGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCAT                                                           >  SRR4302533.4982621/1‑94 (MQ=60)
                                       AAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATAT                                                         >  SRR4302533.4581266/1‑94 (MQ=60)
                                       AAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATAT                                                         <  SRR4302533.2867374/94‑1 (MQ=60)
                                       AAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATAT                                                         <  SRR4302533.4347989/94‑1 (MQ=60)
                                        AGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATG                                                        <  SRR4302533.5640815/94‑1 (MQ=60)
                                        AGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATG                                                        <  SRR4302533.3588847/94‑1 (MQ=60)
                                        AGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATG                                                        >  SRR4302533.3826946/1‑94 (MQ=60)
                                         GATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGA                                                       <  SRR4302533.1980070/94‑1 (MQ=60)
                                          ATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAA                                                      >  SRR4302533.4625479/1‑94 (MQ=60)
                                           TATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAG                                                     <  SRR4302533.5649186/94‑1 (MQ=60)
                                            ATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGT                                                    <  SRR4302533.3592039/94‑1 (MQ=60)
                                              CTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGG                                                  >  SRR4302533.5999526/1‑94 (MQ=60)
                                                CTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAA                                                <  SRR4302533.5852293/94‑1 (MQ=60)
                                                CTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAA                                                >  SRR4302533.1566772/1‑94 (MQ=60)
                                                CTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAA                                                <  SRR4302533.5748539/94‑1 (MQ=60)
                                                CTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAA                                                <  SRR4302533.3439425/94‑1 (MQ=60)
                                                  TGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAAT                                              >  SRR4302533.245683/1‑94 (MQ=60)
                                                   GTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATC                                             >  SRR4302533.3749835/1‑94 (MQ=60)
                                                    TAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCG                                            <  SRR4302533.4484278/94‑1 (MQ=60)
                                                     AGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGT                                           >  SRR4302533.3810565/1‑94 (MQ=60)
                                                     AGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGT                                           <  SRR4302533.1360013/94‑1 (MQ=60)
                                                      GTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTT                                          >  SRR4302533.5714952/1‑94 (MQ=60)
                                                      GTTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTT                                          <  SRR4302533.2793792/94‑1 (MQ=60)
                                                       TTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTT                                         <  SRR4302533.6866/94‑1 (MQ=60)
                                                       TTCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTT                                         >  SRR4302533.2308799/1‑94 (MQ=60)
                                                        TCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTT                                        <  SRR4302533.4713583/94‑1 (MQ=60)
                                                        TCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTT                                        <  SRR4302533.2885025/94‑1 (MQ=60)
                                                        TCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTT                                        <  SRR4302533.367420/94‑1 (MQ=60)
                                                        TCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTT                                        >  SRR4302533.4060510/1‑94 (MQ=60)
                                                        TCCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTT                                        >  SRR4302533.2212924/1‑94 (MQ=60)
                                                         CCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTC                                       >  SRR4302533.1830450/1‑94 (MQ=60)
                                                         CCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTC                                       <  SRR4302533.4495602/94‑1 (MQ=60)
                                                         CCTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGCACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTC                                       >  SRR4302533.3389551/1‑94 (MQ=60)
                                                          CTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCA                                      <  SRR4302533.189407/94‑1 (MQ=60)
                                                          CTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCA                                      >  SRR4302533.3042466/1‑94 (MQ=60)
                                                          CTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCA                                      >  SRR4302533.3754265/1‑94 (MQ=60)
                                                          CTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCA                                      <  SRR4302533.2572795/94‑1 (MQ=60)
                                                          CTGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCA                                      >  SRR4302533.3686951/1‑94 (MQ=60)
                                                           TGTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCAT                                     <  SRR4302533.3550551/94‑1 (MQ=60)
                                                            GTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATA                                    >  SRR4302533.2940721/1‑94 (MQ=60)
                                                            GTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATA                                    >  SRR4302533.3840862/1‑94 (MQ=60)
                                                            GTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATA                                    <  SRR4302533.5140985/94‑1 (MQ=60)
                                                            GTCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATA                                    >  SRR4302533.1442080/1‑94 (MQ=60)
                                                             TCTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAA                                   <  SRR4302533.5312813/94‑1 (MQ=60)
                                                              CTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAA                                  >  SRR4302533.1855328/1‑94 (MQ=60)
                                                              CTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAA                                  >  SRR4302533.3676134/1‑94 (MQ=60)
                                                              CTTCAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAA                                  >  SRR4302533.129403/1‑94 (MQ=60)
                                                                 CAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGA                               <  SRR4302533.4372090/94‑1 (MQ=60)
                                                                 CAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGA                               >  SRR4302533.2640262/1‑94 (MQ=60)
                                                                 CAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGA                               <  SRR4302533.3751014/94‑1 (MQ=60)
                                                                 CAATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGA                               <  SRR4302533.1184045/94‑1 (MQ=60)
                                                                  AATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAACTCGAAGAATTTAAGGCATATGAAGTGGAAATCGTTTTCATAAATGAC                              >  SRR4302533.6211811/1‑94 (MQ=60)
                                                                   ATGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACG                             >  SRR4302533.2541569/1‑94 (MQ=60)
                                                                    TGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGG                            >  SRR4302533.5428374/1‑94 (MQ=60)
                                                                    TGAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGG                            >  SRR4302533.5428375/1‑94 (MQ=60)
                                                                     GAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGCATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGC                           <  SRR4302533.3403060/94‑1 (MQ=60)
                                                                     GAAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATTGTTTTCATAAATGACGGC                           >  SRR4302533.3606518/1‑94 (MQ=60)
                                                                      AAGAAGAAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCA                          >  SRR4302533.5546884/1‑94 (MQ=60)
                                                                            AAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAG                    <  SRR4302533.2153850/94‑1 (MQ=60)
                                                                            AAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAG                    <  SRR4302533.5718789/94‑1 (MQ=60)
                                                                            AAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAG                    <  SRR4302533.177843/94‑1 (MQ=60)
                                                                            AAGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAG                    <  SRR4302533.2569324/94‑1 (MQ=60)
                                                                             AGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGA                   >  SRR4302533.4514645/1‑94 (MQ=60)
                                                                             AGCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGA                   <  SRR4302533.396415/94‑1 (MQ=60)
                                                                              GCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGAC                  <  SRR4302533.2031470/94‑1 (MQ=60)
                                                                              GCGATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGAC                  <  SRR4302533.3294785/94‑1 (MQ=60)
                                                                                GATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGC                >  SRR4302533.5632293/1‑94 (MQ=60)
                                                                                GATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGC                >  SRR4302533.5286357/1‑94 (MQ=60)
                                                                                 ATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCT               >  SRR4302533.5499261/1‑94 (MQ=60)
                                                                                 ATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCT               >  SRR4302533.5647504/1‑94 (MQ=60)
                                                                                 ATACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCT               >  SRR4302533.4815231/1‑94 (MQ=60)
                                                                                  TACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTA              <  SRR4302533.4043926/94‑1 (MQ=60)
                                                                                  TACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTA              >  SRR4302533.1277718/1‑94 (MQ=60)
                                                                                   ACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTAC             <  SRR4302533.3726928/94‑1 (MQ=60)
                                                                                   ACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTAC             >  SRR4302533.258869/1‑94 (MQ=60)
                                                                                   ACCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTAC             >  SRR4302533.3463546/1‑94 (MQ=60)
                                                                                    CCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACG            <  SRR4302533.2395792/94‑1 (MQ=60)
                                                                                    CCAATTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACG            <  SRR4302533.932361/94‑1 (MQ=60)
                                                                                      aatTTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGA          >  SRR4302533.4336564/4‑94 (MQ=60)
                                                                                      aatTTTTTTATAAAACGGTACGTGAATGCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGA          <  SRR4302533.3032098/91‑1 (MQ=60)
                                                                                      AT‑TTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAG         <  SRR4302533.5685586/94‑1 (MQ=60)
                                                                                         TTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTC       <  SRR4302533.3187723/94‑1 (MQ=60)
                                                                                         TTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTC       >  SRR4302533.508494/1‑94 (MQ=60)
                                                                                          TTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCA      >  SRR4302533.4016939/1‑94 (MQ=60)
                                                                                           TTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAA     >  SRR4302533.2203436/1‑94 (MQ=60)
                                                                                           TTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAA     >  SRR4302533.2509830/1‑94 (MQ=60)
                                                                                            TTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAAT    <  SRR4302533.4081875/94‑1 (MQ=60)
                                                                                             TTATAAGACGGTACGTTAATTCAAAGAATTGAAGTAATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAATC   <  SRR4302533.3937428/94‑1 (MQ=60)
                                                                                             TTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAATC   >  SRR4302533.5608518/1‑94 (MQ=60)
                                                                                             TTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAATC   <  SRR4302533.3727508/94‑1 (MQ=60)
                                                                                              TATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAATCA  <  SRR4302533.1161331/94‑1 (MQ=60)
                                                                                              |                                                                                             
CGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCTTCAATGAAGAAGAAGCGATACCAA‑TTTTTTATAAAACGGTACGTGAATTCGAAGAATTGAAGTCATATGAAGTGGAAATCGTTTTCATAAATGACGGCAGCAAAGACGCTACGGAGTCAATCA  >  NC_000913/2466198‑2466384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑