Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,470,298 A→G I37T (ATT→ACT)  b2356 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,2980AG99.6% 895.9 / ‑5.0 275I37T (ATT→ACT) b2356hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (161/113);  total (162/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 279 total aligned reads displayed.

GCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCC  >  NC_000913/2470208‑2470387
                                                                                          |                                                                                         
gcgTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGtt                                                                                         <  1:2643216/93‑1 (MQ=255)
gcgTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGtt                                                                                         >  2:7806198/1‑93 (MQ=255)
  gTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTcc                                                                                       >  1:4971910/1‑93 (MQ=255)
  gTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGAGTTAGTTcc                                                                                       >  2:3129990/1‑93 (MQ=255)
     gTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCa                                                                                    <  1:886233/93‑1 (MQ=255)
        ttCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGaa                                                                                 >  2:5984063/1‑93 (MQ=255)
          ccGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGaaaa                                                                               <  1:9178342/93‑1 (MQ=255)
            gCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtg                                                                             >  2:7756197/1‑93 (MQ=255)
             cAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTGCCCAGAAAAtgt                                                                            >  1:5353776/1‑93 (MQ=255)
             cAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgt                                                                            <  2:8935858/93‑1 (MQ=255)
              aGTGTAATACGCGGCACGTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgtt                                                                           <  1:3507638/93‑1 (MQ=255)
              aGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgtt                                                                           >  2:4504896/1‑93 (MQ=255)
              aGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgtt                                                                           <  1:1634872/93‑1 (MQ=255)
               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          <  1:5559069/93‑1 (MQ=255)
               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          <  2:7929894/93‑1 (MQ=255)
               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          <  1:6708822/93‑1 (MQ=255)
               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          >  2:5881521/1‑93 (MQ=255)
                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  2:4415463/1‑93 (MQ=255)
                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  1:5951885/1‑93 (MQ=255)
                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  2:9054616/1‑93 (MQ=255)
                  tAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTcg                                                                       >  2:3550042/1‑92 (MQ=255)
                  tAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTcc                                                                       <  2:5776261/93‑1 (MQ=255)
                     tACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGc                                                                    >  1:6538599/1‑93 (MQ=255)
                      aCGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCg                                                                   <  1:578462/93‑1 (MQ=255)
                        gcgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTg                                                                 <  1:2825344/93‑1 (MQ=255)
                         cgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgc                                                                <  2:7123034/93‑1 (MQ=255)
                          ggCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgcg                                                               >  2:2820195/1‑93 (MQ=255)
                          ggCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgcg                                                               >  2:506004/1‑93 (MQ=255)
                           gCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgcgc                                                              >  1:9926600/1‑93 (MQ=255)
                            cacaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGcc                                                             >  1:7531474/1‑93 (MQ=255)
                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            <  2:6506874/93‑1 (MQ=255)
                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            >  2:2182790/1‑93 (MQ=255)
                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            >  1:7778795/1‑93 (MQ=255)
                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            >  1:7677805/1‑93 (MQ=255)
                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAc                                                           <  2:105545/93‑1 (MQ=255)
                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAc                                                           <  1:144599/93‑1 (MQ=255)
                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAc                                                           <  1:7303828/93‑1 (MQ=255)
                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGCTCCCCAGACAATGTTGACCGGCGTGCGCCAc                                                           >  2:6534356/1‑93 (MQ=255)
                                 ttGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGa                                                        >  1:4907592/1‑93 (MQ=255)
                                  tGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGAt                                                       <  2:522773/93‑1 (MQ=255)
                                  tGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGAt                                                       <  2:3061035/93‑1 (MQ=255)
                                      gTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGcc                                                   >  2:1717397/1‑93 (MQ=255)
                                      gTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGcc                                                   >  1:4309338/1‑93 (MQ=255)
                                       ttATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGccc                                                  >  2:5352921/1‑93 (MQ=255)
                                         aTCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAc                                                <  2:4055424/93‑1 (MQ=255)
                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               >  2:415275/1‑93 (MQ=255)
                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               >  1:1014425/1‑93 (MQ=255)
                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               <  1:9387782/93‑1 (MQ=255)
                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               >  1:9088607/1‑93 (MQ=255)
                                           cACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActt                                              >  2:5421915/1‑93 (MQ=255)
                                            aCCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAtttc                                             >  2:2736154/1‑93 (MQ=255)
                                             ccATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcc                                            >  2:9215908/1‑93 (MQ=255)
                                              cATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcct                                           >  1:4817225/1‑93 (MQ=255)
                                               aTCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcctt                                          <  2:8699558/93‑1 (MQ=255)
                                                tCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtccttc                                         >  2:1414194/1‑93 (MQ=255)
                                                 cAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCa                                        <  1:576663/93‑1 (MQ=255)
                                                  aGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAg                                       >  2:7712702/1‑93 (MQ=255)
                                                   gTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGt                                      >  2:4345875/1‑93 (MQ=255)
                                                    tAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGtt                                     >  2:4575062/1‑93 (MQ=255)
                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  >  1:3808938/1‑93 (MQ=255)
                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  >  1:7999877/1‑93 (MQ=255)
                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  >  1:1712174/1‑93 (MQ=255)
                                                           aTCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGc                              <  1:3079799/93‑1 (MQ=255)
                                                           aTCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGc                              >  2:4716946/1‑93 (MQ=255)
                                                            tCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCt                             >  2:968857/1‑93 (MQ=255)
                                                            tCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCt                             <  1:1795594/93‑1 (MQ=255)
                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCtc                            >  1:7104554/1‑92 (MQ=255)
                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTg                            <  2:3342218/93‑1 (MQ=255)
                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTg                            >  1:2437779/1‑93 (MQ=255)
                                                              cAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTgg                           >  1:2986365/1‑93 (MQ=255)
                                                               aGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGt                          <  2:493637/93‑1 (MQ=255)
                                                               aGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGt                          >  1:9773292/1‑93 (MQ=255)
                                                                 aaCAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGttt                        >  2:3940065/1‑93 (MQ=255)
                                                                 aaCAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGttt                        >  1:4058256/1‑93 (MQ=255)
                                                                 aaCAAACGGGCCAAACAGGGAGTTAGTTTCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATAGCCCACTTCCTTCAGTTCATGCGATGGgtt                        >  2:3760304/1‑93 (MQ=255)
                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      >  1:3386150/1‑93 (MQ=255)
                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      >  1:6272346/1‑93 (MQ=255)
                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      <  1:8584815/93‑1 (MQ=255)
                                                                    aaaCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGtt                     >  2:9383748/1‑93 (MQ=255)
                                                                    aaaCGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGtt                     >  1:6193251/1‑93 (MQ=255)
                                                                     aaCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTc                    >  1:2348638/1‑93 (MQ=255)
                                                                      aCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTcc                   >  1:2465220/1‑93 (MQ=255)
                                                                      aCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTcc                   >  1:2007443/1‑93 (MQ=255)
                                                                        gggCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGc                 <  1:1151275/93‑1 (MQ=255)
                                                                         ggCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCa                <  2:6137506/93‑1 (MQ=255)
                                                                            cAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCt             >  2:2465842/1‑93 (MQ=255)
                                                                             aaaCAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTc            <  2:2152306/93‑1 (MQ=255)
                                                                               aCAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTcca          >  2:1793116/1‑93 (MQ=255)
                                                                                cAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCGCTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccac         <  1:8129891/93‑1 (MQ=255)
                                                                                 aGGGTGTTAGTTCCCCGGAAAATGGTGTCCGGCGTGCGCCACTCATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacc        <  1:5305384/93‑1 (MQ=255)
                                                                                 aGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacc        <  2:567140/93‑1 (MQ=255)
                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       <  2:7482422/93‑1 (MQ=255)
                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       <  1:95595/93‑1 (MQ=255)
                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       >  1:5284967/1‑93 (MQ=255)
                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       <  2:997129/93‑1 (MQ=255)
                                                                                   ggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAg      <  1:5022496/93‑1 (MQ=255)
                                                                                    gtgtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgc     <  1:5074595/93‑1 (MQ=255)
                                                                                     tgtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgcc    >  2:7345919/1‑92 (MQ=255)
                                                                                      gtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgcgc   <  2:7179740/93‑1 (MQ=255)
                                                                                       ttAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGCTTGGTCCGCAGCTCCACCCGCGcc  >  2:2636510/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCC  >  NC_000913/2470208‑2470387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGGC  >  NC_000913/2470205‑2470391
                                                                                             |                                                                                             
AGCGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAG                                                                                               >  SRR4302540.2186466/1‑94 (MQ=60)
AGCGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAG                                                                                               >  SRR4302540.1558256/1‑94 (MQ=60)
 GCGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGT                                                                                              <  SRR4302540.3243185/94‑1 (MQ=60)
 GCGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGT                                                                                              <  SRR4302540.6223087/94‑1 (MQ=60)
 GCGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGT                                                                                              <  SRR4302540.8868698/94‑1 (MQ=60)
  CGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTT                                                                                             >  SRR4302540.10006573/1‑94 (MQ=60)
  CGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTT                                                                                             >  SRR4302540.2466969/1‑94 (MQ=60)
   GCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTC                                                                                            <  SRR4302540.2671888/94‑1 (MQ=60)
    CGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCC                                                                                           >  SRR4302540.5025708/1‑94 (MQ=60)
    CGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATACAGAACAAACGGGCAAAACAGGGTGTTAGTTCC                                                                                           >  SRR4302540.5025707/1‑94 (MQ=60)
     GTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCC                                                                                          >  SRR4302540.9272528/1‑94 (MQ=60)
     GTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCC                                                                                          >  SRR4302540.1949782/1‑94 (MQ=60)
     GTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCC                                                                                          >  SRR4302540.5088234/1‑94 (MQ=60)
        GTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAG                                                                                       <  SRR4302540.895635/94‑1 (MQ=60)
         TCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGA                                                                                      >  SRR4302540.3622654/1‑94 (MQ=60)
          CTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAA                                                                                     <  SRR4302540.9328944/94‑1 (MQ=60)
            TCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAA                                                                                   >  SRR4302540.2389888/1‑94 (MQ=60)
             CCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAT                                                                                  <  SRR4302540.6556456/94‑1 (MQ=60)
             CCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAT                                                                                  <  SRR4302540.9555059/94‑1 (MQ=60)
             CCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAT                                                                                  <  SRR4302540.9288424/94‑1 (MQ=60)
              CGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATG                                                                                 <  SRR4302540.3030066/94‑1 (MQ=60)
              CGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATG                                                                                 <  SRR4302540.4389097/94‑1 (MQ=60)
               GCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTGCCCAGAAAATGT                                                                                >  SRR4302540.5412560/1‑94 (MQ=60)
                 AGTGTAATACGCGGCACGTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTG                                                                              <  SRR4302540.3545849/94‑1 (MQ=60)
                 AGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTG                                                                              <  SRR4302540.1652756/94‑1 (MQ=60)
                  GTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGT                                                                             <  SRR4302540.3932100/94‑1 (MQ=60)
                  GTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGT                                                                             <  SRR4302540.6782858/94‑1 (MQ=60)
                  GTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGT                                                                             <  SRR4302540.5620292/94‑1 (MQ=60)
                  GTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGT                                                                             >  SRR4302540.6017710/1‑94 (MQ=60)
                  GTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAAAAAACGGGCCAAACAGGGCGTTAGTTCCCAAGAAAATGTTGC                                                                             >  SRR4302540.2965555/1‑94 (MQ=60)
                    GTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCC                                                                           <  SRR4302540.8913773/94‑1 (MQ=60)
                    GTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCC                                                                           >  SRR4302540.2959989/1‑94 (MQ=60)
                    GTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCC                                                                           <  SRR4302540.5385006/94‑1 (MQ=60)
                    GTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCC                                                                           <  SRR4302540.2017212/94‑1 (MQ=60)
                     TAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCG                                                                          <  SRR4302540.5988403/94‑1 (MQ=60)
                       ATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGC                                                                        >  SRR4302540.7747916/1‑94 (MQ=60)
                       ATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGC                                                                        >  SRR4302540.9259201/1‑94 (MQ=60)
                       ATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGC                                                                        >  SRR4302540.3163818/1‑94 (MQ=60)
                       ATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGC                                                                        >  SRR4302540.6611158/1‑94 (MQ=60)
                         ACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGT                                                                      <  SRR4302540.584729/94‑1 (MQ=60)
                          CGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTG                                                                     <  SRR4302540.9051525/94‑1 (MQ=60)
                           GCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGC                                                                    <  SRR4302540.2855873/94‑1 (MQ=60)
                           GCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGCGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGC                                                                    >  SRR4302540.5071582/1‑94 (MQ=60)
                            CGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCG                                                                   >  SRR4302540.10083509/1‑94 (MQ=60)
                             GGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGC                                                                  >  SRR4302540.7599840/1‑94 (MQ=60)
                             GGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGC                                                                  >  SRR4302540.10042488/1‑94 (MQ=60)
                              GCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCC                                                                 >  SRR4302540.7615802/1‑94 (MQ=60)
                              GCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCC                                                                 >  SRR4302540.7615803/1‑94 (MQ=60)
                               CACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCA                                                                <  SRR4302540.7543341/94‑1 (MQ=60)
                               CACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCA                                                                >  SRR4302540.7764626/1‑94 (MQ=60)
                               CACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCA                                                                >  SRR4302540.7869726/1‑94 (MQ=60)
                               CACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCACAGAAAATGTTGTCCGGCGTGCGCCA                                                                >  SRR4302540.440199/1‑94 (MQ=60)
                                ACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCGC                                                               >  SRR4302540.645721/1‑94 (MQ=60)
                                ACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAC                                                               <  SRR4302540.1486999/94‑1 (MQ=60)
                                ACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAC                                                               >  SRR4302540.1593987/1‑94 (MQ=60)
                                ACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAC                                                               <  SRR4302540.3094369/94‑1 (MQ=60)
                                ACATTTGGCGGTATCACCATCAGTAAACAGATACAGAACAAACTGGCCAAACAGGGTGCTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAC                                                               >  SRR4302540.2090037/1‑94 (MQ=60)
                                 CATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACT                                                              <  SRR4302540.7385357/94‑1 (MQ=60)
                                 CATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACT                                                              <  SRR4302540.146103/94‑1 (MQ=60)
                                   TTTGGCGTTATCTCCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGA                                                            >  SRR4302540.4960420/1‑94 (MQ=60)
                                   TTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGA                                                            >  SRR4302540.4960421/1‑94 (MQ=60)
                                     TGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATC                                                          <  SRR4302540.1696526/94‑1 (MQ=60)
                                      GGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCG                                                         >  SRR4302540.8437284/1‑94 (MQ=60)
                                      GGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCG                                                         >  SRR4302540.8437286/1‑94 (MQ=60)
                                       GCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGC                                                        >  SRR4302540.5405092/1‑94 (MQ=60)
                                        CGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCC                                                       >  SRR4302540.4356246/1‑94 (MQ=60)
                                         GTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCC                                                      >  SRR4302540.3848115/1‑94 (MQ=60)
                                         GTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCC                                                      >  SRR4302540.6245067/1‑94 (MQ=60)
                                          TTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCA                                                     <  SRR4302540.504086/94‑1 (MQ=60)
                                           TATCACCATCAGTAAACAGATCCAGACCAAACGGGCCAACCAGGGTGTTAGTT‑CCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACT                                                   <  SRR4302540.811234/94‑1 (MQ=60)
                                           TATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGCCGTGCGACACTGAGCGCCCAC                                                    >  SRR4302540.2697915/1‑94 (MQ=60)
                                            ATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACT                                                   >  SRR4302540.9197793/1‑94 (MQ=60)
                                            ATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACT                                                   >  SRR4302540.1024710/1‑94 (MQ=60)
                                             TCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCGACTT                                                  >  SRR4302540.10008776/1‑94 (MQ=60)
                                             TCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTT                                                  <  SRR4302540.9499511/94‑1 (MQ=60)
                                             TCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTT                                                  >  SRR4302540.2396942/1‑94 (MQ=60)
                                               ACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCC                                                >  SRR4302540.6311990/1‑94 (MQ=60)
                                               ACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCC                                                >  SRR4302540.5583440/1‑94 (MQ=60)
                                                CCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCT                                               >  SRR4302540.4868793/1‑94 (MQ=60)
                                                  ATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTC                                             >  SRR4302540.5196201/1‑94 (MQ=60)
                                                   TCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCA                                            <  SRR4302540.9844821/94‑1 (MQ=60)
                                                    CAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAG                                           <  SRR4302540.582911/94‑1 (MQ=60)
                                                      GTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTT                                         >  SRR4302540.9413995/1‑94 (MQ=60)
                                                      GTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTT                                         >  SRR4302540.9142491/1‑94 (MQ=60)
                                                      GTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTT                                         >  SRR4302540.5754279/1‑94 (MQ=60)
                                                       TAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTC                                        >  SRR4302540.6301263/1‑94 (MQ=60)
                                                         AACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAT                                      >  SRR4302540.3850421/1‑94 (MQ=60)
                                                         AACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAT                                      >  SRR4302540.8092991/1‑94 (MQ=60)
                                                         AACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAT                                      <  SRR4302540.7921768/94‑1 (MQ=60)
                                                         AACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAT                                      <  SRR4302540.3897941/94‑1 (MQ=60)
                                                         AACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAT                                      >  SRR4302540.1730672/1‑94 (MQ=60)
                                                         AACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAT                                      >  SRR4302540.1730671/1‑94 (MQ=60)
                                                          ACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATG                                     >  SRR4302540.165453/1‑94 (MQ=60)
                                                           CAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGG                                    >  SRR4302540.4307534/1‑94 (MQ=60)
                                                            AGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGG                                   >  SRR4302540.5730813/1‑94 (MQ=60)
                                                             GATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGT                                  >  SRR4302540.2094556/1‑94 (MQ=60)
                                                             GATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGC                                  >  SRR4302540.8061805/1‑94 (MQ=60)
                                                             GATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGC                                  >  SRR4302540.5117483/1‑94 (MQ=60)
                                                             GATCCAGAACAAACGGGCCAAACAGGGGGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGC                                  >  SRR4302540.9581271/1‑94 (MQ=60)
                                                              ATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCT                                 <  SRR4302540.7028438/94‑1 (MQ=60)
                                                              ATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCT                                 <  SRR4302540.3113596/94‑1 (MQ=60)
                                                               TCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTG                                <  SRR4302540.1815124/94‑1 (MQ=60)
                                                               TCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTG                                >  SRR4302540.2464032/1‑94 (MQ=60)
                                                               TCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTG                                >  SRR4302540.7271777/1‑94 (MQ=60)
                                                               TCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTC                                >  SRR4302540.7183602/1‑94 (MQ=60)
                                                                CCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGG                               >  SRR4302540.3019307/1‑94 (MQ=60)
                                                                 CAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGT                              >  SRR4302540.9887866/1‑94 (MQ=60)
                                                                   GAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTT                            >  SRR4302540.4102011/1‑94 (MQ=60)
                                                                    AACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTT                           >  SRR4302540.3482107/1‑94 (MQ=60)
                                                                    AACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTG                           <  SRR4302540.2708501/94‑1 (MQ=60)
                                                                     ACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGT                          >  SRR4302540.6342132/1‑94 (MQ=60)
                                                                     ACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGT                          >  SRR4302540.3423102/1‑94 (MQ=60)
                                                                      CAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTT                         <  SRR4302540.8684619/94‑1 (MQ=60)
                                                                      CAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTT                         >  SRR4302540.6262210/1‑94 (MQ=60)
                                                                       AAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTC                        >  SRR4302540.2373933/1‑94 (MQ=60)
                                                                        AACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCC                       <  SRR4302540.4309057/94‑1 (MQ=60)
                                                                        AACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCC                       >  SRR4302540.10044135/1‑94 (MQ=60)
                                                                        AACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCC                       >  SRR4302540.2491754/1‑94 (MQ=60)
                                                                        AACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCC                       >  SRR4302540.2491753/1‑94 (MQ=60)
                                                                        AACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCC                       >  SRR4302540.2029159/1‑94 (MQ=60)
                                                                         ACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCG                      <  SRR4302540.6634249/94‑1 (MQ=60)
                                                                           GGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCA                    <  SRR4302540.1163273/94‑1 (MQ=60)
                                                                               CAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTC                >  SRR4302540.3127253/1‑94 (MQ=60)
                                                                                AAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCC               <  SRR4302540.585285/94‑1 (MQ=60)
                                                                                AAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCC               <  SRR4302540.501336/94‑1 (MQ=60)
                                                                                 AACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCA              <  SRR4302540.7828102/94‑1 (MQ=60)
                                                                                   CAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCGCTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACC            <  SRR4302540.8224259/94‑1 (MQ=60)
                                                                                   CAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACC            <  SRR4302540.6215007/94‑1 (MQ=60)
                                                                                   CAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACC            >  SRR4302540.295349/1‑94 (MQ=60)
                                                                                    AGGGTGTTAGTTCCCCGGAAAATGGTGTCCGGCGTGCGCCACTCATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCA           <  SRR4302540.5363438/94‑1 (MQ=60)
                                                                                    AGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCA           >  SRR4302540.5342717/1‑94 (MQ=60)
                                                                                    AGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCA           >  SRR4302540.535040/1‑94 (MQ=60)
                                                                                    AGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCA           >  SRR4302540.5840035/1‑94 (MQ=60)
                                                                                    AGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCA           <  SRR4302540.5363437/94‑1 (MQ=60)
                                                                                     GGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAG          <  SRR4302540.8116123/94‑1 (MQ=60)
                                                                                     GGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAG          <  SRR4302540.96607/94‑1 (MQ=60)
                                                                                      GGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGC         <  SRR4302540.5076866/94‑1 (MQ=60)
                                                                                       GTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCG        <  SRR4302540.5129458/94‑1 (MQ=60)
                                                                                       GTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCG        >  SRR4302540.1079377/1‑94 (MQ=60)
                                                                                       GTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCG        <  SRR4302540.5501351/94‑1 (MQ=60)
                                                                                       GTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCG        >  SRR4302540.2477423/1‑94 (MQ=60)
                                                                                        TGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGC       >  SRR4302540.1401372/1‑94 (MQ=60)
                                                                                         GTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCC      >  SRR4302540.7288657/1‑94 (MQ=60)
                                                                                          TTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCT     <  SRR4302540.1194067/94‑1 (MQ=60)
                                                                                          TTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCT     <  SRR4302540.6808626/94‑1 (MQ=60)
                                                                                            AGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGG   >  SRR4302540.7441022/1‑94 (MQ=60)
                                                                                            AGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGG   <  SRR4302540.7897420/94‑1 (MQ=60)
                                                                                             GTTCCCCAGACAATGTTGTCCGGCGTGCCCCACTGATCGCCCGCTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGGC  <  SRR4302540.3527449/94‑1 (MQ=60)
                                                                                             GTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGGC  <  SRR4302540.3027044/94‑1 (MQ=60)
                                                                                             GTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGGC  >  SRR4302540.1118093/1‑94 (MQ=60)
                                                                                             GTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGGC  <  SRR4302540.1973412/94‑1 (MQ=60)
                                                                                             GTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGGC  <  SRR4302540.3527444/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCGCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCCTGGC  >  NC_000913/2470205‑2470391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑