Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,562,351 G→A G118S (GGC→AGC)  b2449 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,562,3510GA100.0% 848.3 / NA 225G118S (GGC→AGC) b2449hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (103/122);  total (103/122)

Only 100 of 229 total aligned reads displayed.

ATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGG  >  NC_000913/2562261‑2562440
                                                                                          |                                                                                         
aTCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGc                                                                                         <  1:1216441/93‑1 (MQ=255)
aTCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGc                                                                                         >  1:4839810/1‑93 (MQ=255)
aTCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGc                                                                                         >  1:43978/1‑93 (MQ=255)
aTCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGc                                                                                         <  1:3364569/93‑1 (MQ=255)
aTCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGc                                                                                         >  2:381355/1‑93 (MQ=255)
 tCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCt                                                                                        >  1:13617/1‑93 (MQ=255)
  ccTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCtt                                                                                       <  1:4276118/93‑1 (MQ=255)
  ccTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCtt                                                                                       >  2:5038319/1‑93 (MQ=255)
   cTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCttt                                                                                      >  1:1808765/1‑93 (MQ=255)
   cTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCttt                                                                                      <  2:5962961/93‑1 (MQ=255)
    tGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTg                                                                                     <  2:3099746/93‑1 (MQ=255)
    tGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTg                                                                                     <  2:3655897/93‑1 (MQ=255)
    tGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTg                                                                                     >  2:5806103/1‑93 (MQ=255)
     ggTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGt                                                                                    <  2:4984042/93‑1 (MQ=255)
       tAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGttt                                                                                  <  1:2212266/93‑1 (MQ=255)
        aaaaTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGtttt                                                                                 <  1:252049/93‑1 (MQ=255)
        aaaaTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGtttt                                                                                 >  2:4449317/1‑93 (MQ=255)
        aaaaTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGtttt                                                                                 <  1:4731880/93‑1 (MQ=255)
         aaaTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTg                                                                                <  2:1336207/93‑1 (MQ=255)
          aaTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTgg                                                                               >  1:2101843/1‑93 (MQ=255)
          aaTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTgg                                                                               <  2:177795/93‑1 (MQ=255)
          aaTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTgg                                                                               >  2:6130322/1‑93 (MQ=255)
           aTACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggt                                                                              <  2:6131916/93‑1 (MQ=255)
            tACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggta                                                                             <  1:2865582/93‑1 (MQ=255)
            tACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggta                                                                             <  1:1955425/93‑1 (MQ=255)
             aCCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggtag                                                                            <  1:1641813/93‑1 (MQ=255)
             aCCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggtag                                                                            <  2:3570320/93‑1 (MQ=255)
              ccATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAAAAAACAACAGCTTTGTTTTggtagg                                                                           >  2:2033333/1‑93 (MQ=255)
               cATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggtaggt                                                                          <  1:6250427/93‑1 (MQ=255)
               cATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggtaggt                                                                          <  2:1288670/93‑1 (MQ=255)
                aTTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTggtaggta                                                                         >  1:4158756/1‑93 (MQ=255)
                 tttCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGgtaggtag                                                                        >  1:4197555/1‑93 (MQ=255)
                 tttCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGgtaggtag                                                                        <  2:802915/93‑1 (MQ=255)
                 tttCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGgtaggtag                                                                        >  1:3322332/1‑93 (MQ=255)
                   tCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTagag                                                                      <  1:3115124/93‑1 (MQ=255)
                   tCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTagag                                                                      <  1:258974/93‑1 (MQ=255)
                   tCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGAagag                                                                      <  2:1606366/93‑1 (MQ=255)
                       cAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGGAtt                                                                  >  2:2536714/1‑93 (MQ=255)
                        aaTGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTAttt                                                                 <  2:2305724/93‑1 (MQ=255)
                        aaTGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTAttt                                                                 >  2:739168/1‑93 (MQ=255)
                         aTGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTg                                                                >  1:3572332/1‑93 (MQ=255)
                          tGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGa                                                               >  1:628788/1‑93 (MQ=255)
                             aaCTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGaaaa                                                            >  1:2500653/1‑93 (MQ=255)
                              aCTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGaaaaa                                                           <  2:4434380/93‑1 (MQ=255)
                              aCTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGaaaaa                                                           >  1:5028158/1‑93 (MQ=255)
                              aCTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGaaaaa                                                           <  1:4772285/93‑1 (MQ=255)
                              aCTGAGTTCAAACTTAAAGCGGCAGAGGTCAGAGAATGCCTGAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGaaaaa                                                           <  1:3179666/93‑1 (MQ=255)
                                 gAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGt                                                        >  2:2394806/1‑93 (MQ=255)
                                  aGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTa                                                       <  1:1677550/93‑1 (MQ=255)
                                   gTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTaa                                                      >  2:2787407/1‑93 (MQ=255)
                                   gTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTaa                                                      >  1:3004244/1‑93 (MQ=255)
                                   gTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTaa                                                      <  1:6301832/93‑1 (MQ=255)
                                    ttCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAat                                                     <  1:4002012/93‑1 (MQ=255)
                                    ttCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAat                                                     <  2:5339512/93‑1 (MQ=255)
                                     tCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAatt                                                    >  2:5196154/1‑93 (MQ=255)
                                      cAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattg                                                   <  1:1611488/93‑1 (MQ=255)
                                       aaaCTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattga                                                  >  2:1655096/1‑93 (MQ=255)
                                       aaaCTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattga                                                  >  2:5509532/1‑93 (MQ=255)
                                        aaCTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTATGTAGAGTATTTGAAAAACGTAattgat                                                 >  1:3770119/1‑93 (MQ=255)
                                        aaCTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattgat                                                 <  1:1068275/93‑1 (MQ=255)
                                         aCTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattgatt                                                <  1:5001039/93‑1 (MQ=255)
                                          cTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattgattg                                               <  2:1836386/93‑1 (MQ=255)
                                          cTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAattgattg                                               <  2:2908694/93‑1 (MQ=255)
                                           ttAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGc                                              >  1:2062981/1‑93 (MQ=255)
                                             aaaGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGa                                            <  1:4123345/93‑1 (MQ=255)
                                               aGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAAt                                          <  1:6031416/93‑1 (MQ=255)
                                                gCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATa                                         >  2:5732425/1‑93 (MQ=255)
                                                gCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATa                                         <  1:5822148/93‑1 (MQ=255)
                                                gCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATa                                         >  2:312463/1‑93 (MQ=255)
                                                 cGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAg                                        <  2:2198860/93‑1 (MQ=255)
                                                        ggTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCaacaac                                 >  1:4537959/1‑93 (MQ=255)
                                                         gTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACt                                >  1:2716435/1‑93 (MQ=255)
                                                         gTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACt                                >  1:4890662/1‑93 (MQ=255)
                                                          tCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTa                               <  1:5355656/93‑1 (MQ=255)
                                                           cAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTaa                              <  1:2294365/93‑1 (MQ=255)
                                                                 aTGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAAcc                        <  2:2643275/93‑1 (MQ=255)
                                                                  tGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCa                       <  1:5317395/93‑1 (MQ=255)
                                                                  tGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCa                       <  1:3310145/93‑1 (MQ=255)
                                                                  tGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCa                       <  1:5492691/93‑1 (MQ=255)
                                                                  tGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCa                       >  2:5840926/1‑93 (MQ=255)
                                                                   gcgcCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCaa                      >  2:4478613/1‑93 (MQ=255)
                                                                   gcgcCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCaa                      <  2:1918816/93‑1 (MQ=255)
                                                                    cgcCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAAt                     <  2:6246054/93‑1 (MQ=255)
                                                                    cgcCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAAt                     <  2:1014336/93‑1 (MQ=255)
                                                                     gcCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATa                    <  2:327810/93‑1 (MQ=255)
                                                                       cAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATaca                  <  1:2020229/93‑1 (MQ=255)
                                                                       cAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATaca                  >  1:3677436/1‑93 (MQ=255)
                                                                        aaCGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATacac                 <  2:3943956/93‑1 (MQ=255)
                                                                        aaCGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATacac                 >  1:5693046/1‑93 (MQ=255)
                                                                        aaCGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATacac                 >  2:2724741/1‑93 (MQ=255)
                                                                        aaCGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATacac                 >  2:870939/1‑93 (MQ=255)
                                                                         aCGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACAcc                <  1:1474291/93‑1 (MQ=255)
                                                                          cGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACAccc               <  2:1501664/93‑1 (MQ=255)
                                                                                 gAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCt        >  1:5179435/1‑93 (MQ=255)
                                                                                 gAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCt        >  1:5832091/1‑93 (MQ=255)
                                                                                  aaaaCAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTc       >  2:4818040/1‑93 (MQ=255)
                                                                                   aaaCAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCg      <  2:5561218/93‑1 (MQ=255)
                                                                                    aacaacAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGc     <  2:2935448/93‑1 (MQ=255)
                                                                                     acaacaGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGcc    <  2:2588882/93‑1 (MQ=255)
                                                                                       aacAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCgg  <  2:1303633/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGG  >  NC_000913/2562261‑2562440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTT  >  NC_000913/2562258‑2562444
                                                                                             |                                                                                             
AACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACA                                                                                               <  SRR4302533.2213614/94‑1 (MQ=60)
AACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACA                                                                                               <  SRR4302533.754998/94‑1 (MQ=60)
 ACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAG                                                                                              >  SRR4302533.5700589/1‑94 (MQ=60)
 ACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAG                                                                                              <  SRR4302533.1948925/94‑1 (MQ=60)
 ACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAG                                                                                              >  SRR4302533.5142164/1‑94 (MQ=60)
 ACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAG                                                                                              <  SRR4302533.2898217/94‑1 (MQ=60)
  CATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGC                                                                                             >  SRR4302533.4869145/1‑94 (MQ=60)
  CATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGC                                                                                             >  SRR4302533.44279/1‑94 (MQ=60)
   ATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCT                                                                                            >  SRR4302533.4435640/1‑94 (MQ=60)
   ATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCT                                                                                            <  SRR4302533.3646327/94‑1 (MQ=60)
   ATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCT                                                                                            <  SRR4302533.3385355/94‑1 (MQ=60)
   ATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCT                                                                                            <  SRR4302533.1223747/94‑1 (MQ=60)
   ATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCT                                                                                            >  SRR4302533.13719/1‑94 (MQ=60)
     CCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTT                                                                                          <  SRR4302533.4786869/94‑1 (MQ=60)
     CCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTT                                                                                          >  SRR4302533.4626780/1‑94 (MQ=60)
     CCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTT                                                                                          >  SRR4302533.1819770/1‑94 (MQ=60)
     CCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTT                                                                                          <  SRR4302533.4908983/94‑1 (MQ=60)
     CCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTT                                                                                          <  SRR4302533.4301837/94‑1 (MQ=60)
       TGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGT                                                                                        <  SRR4302533.3374657/94‑1 (MQ=60)
       TGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGT                                                                                        >  SRR4302533.1427457/1‑94 (MQ=60)
         GTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTT                                                                                      <  SRR4302533.5532919/94‑1 (MQ=60)
          TAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTT                                                                                     <  SRR4302533.2225913/94‑1 (MQ=60)
           AAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTG                                                                                    <  SRR4302533.253600/94‑1 (MQ=60)
           AAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTG                                                                                    <  SRR4302533.4760567/94‑1 (MQ=60)
            AAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGG                                                                                   >  SRR4302533.2114805/1‑94 (MQ=60)
            AAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGG                                                                                   <  SRR4302533.545021/94‑1 (MQ=60)
             AATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGT                                                                                  <  SRR4302533.2012100/94‑1 (MQ=60)
             AATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGT                                                                                  <  SRR4302533.354208/94‑1 (MQ=60)
              ATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTA                                                                                 >  SRR4302533.5200957/1‑94 (MQ=60)
               TACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAG                                                                                <  SRR4302533.1967339/94‑1 (MQ=60)
               TACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAG                                                                                <  SRR4302533.4257324/94‑1 (MQ=60)
               TACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAG                                                                                >  SRR4302533.157064/1‑94 (MQ=60)
               TACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAG                                                                                <  SRR4302533.2883213/94‑1 (MQ=60)
                ACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGG                                                                               <  SRR4302533.1651758/94‑1 (MQ=60)
                ACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGG                                                                               >  SRR4302533.5263528/1‑94 (MQ=60)
                ACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGG                                                                               <  SRR4302533.2589665/94‑1 (MQ=60)
                  CATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTA                                                                             >  SRR4302533.2742830/1‑94 (MQ=60)
                  CATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTA                                                                             <  SRR4302533.6287821/94‑1 (MQ=60)
                  CATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTA                                                                             >  SRR4302533.4183957/1‑94 (MQ=60)
                  CATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTA                                                                             <  SRR4302533.1491914/94‑1 (MQ=60)
                   ATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAG                                                                            >  SRR4302533.4222964/1‑94 (MQ=60)
                   ATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAG                                                                            >  SRR4302533.3342850/1‑94 (MQ=60)
                      TCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGT                                                                         <  SRR4302533.260547/94‑1 (MQ=60)
                      TCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGT                                                                         <  SRR4302533.3134279/94‑1 (MQ=60)
                       CAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTA                                                                        >  SRR4302533.6116289/1‑94 (MQ=60)
                           AATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTG                                                                    >  SRR4302533.3594344/1‑94 (MQ=60)
                            ATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGA                                                                   >  SRR4302533.632488/1‑94 (MQ=60)
                            ATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGA                                                                   <  SRR4302533.5940384/94‑1 (MQ=60)
                            ATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGA                                                                   <  SRR4302533.5685734/94‑1 (MQ=60)
                               AAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAA                                                                >  SRR4302533.2102493/1‑94 (MQ=60)
                               AAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAA                                                                >  SRR4302533.4327769/1‑94 (MQ=60)
                               AAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAA                                                                >  SRR4302533.2516076/1‑94 (MQ=60)
                                AACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAA                                                               >  SRR4302533.5058606/1‑94 (MQ=60)
                                 ACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAAC                                                              <  SRR4302533.4801207/94‑1 (MQ=60)
                                 ACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAAC                                                              <  SRR4302533.3227646/94‑1 (MQ=60)
                                 ACTGAGTTCAAACTTAAAGCGGCAGAGGTCAGAGAATGCCTGAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAAC                                                              <  SRR4302533.3199226/94‑1 (MQ=60)
                                  CTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACG                                                             >  SRR4302533.4079054/1‑94 (MQ=60)
                                   TGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGT                                                            >  SRR4302533.806647/1‑94 (MQ=60)
                                    GAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTA                                                           <  SRR4302533.1677985/94‑1 (MQ=60)
                                     AGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAA                                                          >  SRR4302533.3022620/1‑94 (MQ=60)
                                     AGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAA                                                          <  SRR4302533.1687706/94‑1 (MQ=60)
                                     AGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAA                                                          <  SRR4302533.841273/94‑1 (MQ=60)
                                      GTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAAT                                                         <  SRR4302533.6339476/94‑1 (MQ=60)
                                      GTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAAT                                                         >  SRR4302533.4205782/1‑94 (MQ=60)
                                      GTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAAT                                                         >  SRR4302533.5861399/1‑94 (MQ=60)
                                       TTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATT                                                        <  SRR4302533.4225717/94‑1 (MQ=60)
                                       TTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATT                                                        >  SRR4302533.2491392/1‑94 (MQ=60)
                                       TTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATT                                                        <  SRR4302533.4026383/94‑1 (MQ=60)
                                        TCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTG                                                       <  SRR4302533.4735144/94‑1 (MQ=60)
                                        TCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTG                                                       <  SRR4302533.487477/94‑1 (MQ=60)
                                         CAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGA                                                      >  SRR4302533.5039253/1‑94 (MQ=60)
                                         CAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGA                                                      <  SRR4302533.1621259/94‑1 (MQ=60)
                                          AAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTATGTAGAGTATTTGAAAAACGTAATTGAT                                                     >  SRR4302533.3793232/1‑94 (MQ=60)
                                           AACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATT                                                    <  SRR4302533.1074561/94‑1 (MQ=60)
                                            ACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTG                                                   <  SRR4302533.3094414/94‑1 (MQ=60)
                                            ACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTG                                                   <  SRR4302533.5031353/94‑1 (MQ=60)
                                             CTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGC                                                  >  SRR4302533.2075700/1‑94 (MQ=60)
                                             CTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGC                                                  <  SRR4302533.1897968/94‑1 (MQ=60)
                                              TTAAAGCGGCAGCGGTCAGAGAATGCGACAACGTAGCAGAAAACACCAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCG                                                 <  SRR4302533.6337474/94‑1 (MQ=60)
                                                AAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAA                                               <  SRR4302533.4148380/94‑1 (MQ=60)
                                                AAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAA                                               >  SRR4302533.3330975/1‑94 (MQ=60)
                                                 AAGCGGCAGCGGTCAGATAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAAT                                              <  SRR4302533.2626753/94‑1 (MQ=60)
                                                  AGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATA                                             <  SRR4302533.6067970/94‑1 (MQ=60)
                                                   GCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAG                                            <  SRR4302533.5857317/94‑1 (MQ=60)
                                                     GGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCA                                          >  SRR4302533.1360016/1‑94 (MQ=60)
                                                      GCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAA                                         <  SRR4302533.5357586/94‑1 (MQ=60)
                                                         GCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAA                                      <  SRR4302533.2535691/94‑1 (MQ=60)
                                                          CGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAAC                                     >  SRR4302533.4565434/1‑94 (MQ=60)
                                                          CGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAAC                                     <  SRR4302533.4155869/94‑1 (MQ=60)
                                                           GGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACT                                    >  SRR4302533.2733113/1‑94 (MQ=60)
                                                           GGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACT                                    >  SRR4302533.296163/1‑94 (MQ=60)
                                                           GGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACT                                    <  SRR4302533.2277988/94‑1 (MQ=60)
                                                           GGTCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACT                                    >  SRR4302533.4920292/1‑94 (MQ=60)
                                                             TCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAA                                  <  SRR4302533.5387805/94‑1 (MQ=60)
                                                             TCAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAA                                  <  SRR4302533.1782033/94‑1 (MQ=60)
                                                              CAGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAA                                 <  SRR4302533.2308567/94‑1 (MQ=60)
                                                               AGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAAC                                >  SRR4302533.248329/1‑94 (MQ=60)
                                                               AGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAAC                                >  SRR4302533.2105168/1‑94 (MQ=60)
                                                               AGAGAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAAC                                >  SRR4302533.1629882/1‑94 (MQ=60)
                                                                  GAATGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAAC                             >  SRR4302533.3140993/1‑94 (MQ=60)
                                                                   AATGCGCCAACGTAGAAGAAAACAACAGCGTTGTTTTGGTAGGTAGAGTATTTGAAAACCGTAATTGATTGCGAATAGCAACAACTAAACAACC                            <  SRR4302533.3528019/94‑1 (MQ=60)
                                                                     TGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAA                          <  SRR4302533.5525822/94‑1 (MQ=60)
                                                                     TGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAA                          <  SRR4302533.3330583/94‑1 (MQ=60)
                                                                     TGCGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAA                          <  SRR4302533.5349261/94‑1 (MQ=60)
                                                                       CGCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATA                        <  SRR4302533.6301769/94‑1 (MQ=60)
                                                                        GCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATAC                       <  SRR4302533.5781032/94‑1 (MQ=60)
                                                                        GCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATAC                       <  SRR4302533.2036702/94‑1 (MQ=60)
                                                                        GCCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATAC                       <  SRR4302533.2132232/94‑1 (MQ=60)
                                                                         CCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACA                      >  SRR4302533.3700038/1‑94 (MQ=60)
                                                                         CCAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACA                      <  SRR4302533.4848904/94‑1 (MQ=60)
                                                                          CAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACAC                     >  SRR4302533.5014432/1‑94 (MQ=60)
                                                                          CAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACAC                     <  SRR4302533.2032670/94‑1 (MQ=60)
                                                                          CAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACAC                     <  SRR4302533.2878073/94‑1 (MQ=60)
                                                                          CAACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACAC                     >  SRR4302533.5727426/1‑94 (MQ=60)
                                                                           AACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACC                    >  SRR4302533.3899234/1‑94 (MQ=60)
                                                                            ACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCC                   <  SRR4302533.1483200/94‑1 (MQ=60)
                                                                            ACGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCC                   <  SRR4302533.3899347/94‑1 (MQ=60)
                                                                             CGTAGCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCG                  <  SRR4302533.6219091/94‑1 (MQ=60)
                                                                                 GCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCC              <  SRR4302533.3710328/94‑1 (MQ=60)
                                                                                 GCAGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCAC              >  SRR4302533.1735669/1‑94 (MQ=60)
                                                                                   AGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCT            >  SRR4302533.5867339/1‑94 (MQ=60)
                                                                                   AGAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCT            >  SRR4302533.5210714/1‑94 (MQ=60)
                                                                                    GAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTC           >  SRR4302533.3150981/1‑94 (MQ=60)
                                                                                    GAAAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTC           <  SRR4302533.1153050/94‑1 (MQ=60)
                                                                                      AAACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGC         >  SRR4302533.5345083/1‑94 (MQ=60)
                                                                                       AACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAGCGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCC        <  SRR4302533.4717863/94‑1 (MQ=60)
                                                                                        ACAACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTNGNCG       >  SRR4302533.2110864/1‑94 (MQ=60)
                                                                                           ACAGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGT    >  SRR4302533.4187221/1‑94 (MQ=60)
                                                                                             AGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTT  <  SRR4302533.4174937/94‑1 (MQ=60)
                                                                                             AGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTT  <  SRR4302533.1631012/94‑1 (MQ=60)
                                                                                             |                                                                                             
AACATCCTGGTAAAATACCATTTCAGCAATGAAACTGAGTTCAAACTTAAAGCGGCAGCGGTCAGAGAATGCGCCAACGTAGCAGAAAACAACGGCTTTGTTTTGGTAGGTAGAGTATTTGAAAAACGTAATTGATTGCGAATAGCAACAACTAAACAACCAATACACCCGGCCCCTCGCCGGGTTT  >  NC_000913/2562258‑2562444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑