Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,563,585	(C)_5→6	coding (1320/1404 nt)	b2451 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,563,580	1	.	C	99.5%	604.3 / ‑5.6	191	D442G (GAC→GGC)	b2451	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (100/89); minor base A (1/0); total (101/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 228 total aligned reads displayed.

GCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAA  >  NC_000913/2563488‑2563667

gCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑t                                                                                           >  1:3626260/1‑93 (MQ=255)

  taaTACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑c                                                                                       <  1:2815025/92‑1 (MQ=255)

  gAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑c                                                                                       >  2:5750727/1‑93 (MQ=255)

   aaTACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑cc                                                                                      >  2:2511567/1‑93 (MQ=255)

   aaTACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑cc                                                                                      <  2:3058826/93‑1 (MQ=255)

    aTACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑ccc                                                                                     >  2:74567/1‑93 (MQ=255)

      aCCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑ccccc                                                                                   >  2:5021583/1‑92 (MQ=255)

      aCCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑ccccc                                                                                   <  2:5141288/93‑2 (MQ=255)

      aCCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑ccccc                                                                                   <  1:2633037/93‑2 (MQ=255)

       ccGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑cccccg                                                                                  <  2:3029765/93‑3 (MQ=255)

       ccGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑cccccg                                                                                  <  1:1031829/93‑3 (MQ=255)

        cGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑cccccgc                                                                                 <  1:4721237/93‑4 (MQ=255)

         gCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑cccccgcg                                                                                >  1:3119190/1‑89 (MQ=255)

         gCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑c‑cccccgcg                                                                                >  1:4424398/1‑89 (MQ=255)

               aggaaggaAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACa                                                                           >  2:4497955/1‑93 (MQ=255)

               aggaaggaAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CTCCCCGCGCGGACa                                                                           >  2:1635730/1‑93 (MQ=255)

                 gaaggaaATGCGGGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCCTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAAt                                                                         >  2:1743414/1‑93 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAAt                                                                         <  2:708071/93‑1 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAAt                                                                         >  1:1997225/1‑93 (MQ=255)

                 gaaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAAt                                                                         >  1:3201823/1‑93 (MQ=255)

                  aaggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATc                                                                        >  1:6109050/1‑93 (MQ=255)

                   aggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCa                                                                       >  2:6043182/1‑93 (MQ=255)

                   aggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCa                                                                       <  1:4076397/93‑1 (MQ=255)

                    ggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCAc                                                                      >  2:20657/1‑93 (MQ=255)

                    ggaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCAc                                                                      <  1:929671/93‑1 (MQ=255)

                     gaaATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACt                                                                     <  1:831855/93‑1 (MQ=255)

                      gaaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAGAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACtt                                                                    <  2:197981/92‑1 (MQ=255)

                       aaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTc                                                                   <  2:1694383/93‑1 (MQ=255)

                       aaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTc                                                                   <  1:4818580/93‑1 (MQ=255)

                       aaTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTc                                                                   <  2:2892475/93‑1 (MQ=255)

                        aTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCg                                                                  <  2:1317415/93‑1 (MQ=255)

                        aTGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCg                                                                  >  1:2483571/1‑93 (MQ=255)

                         tGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGt                                                                 >  2:5941947/1‑93 (MQ=255)

                           cGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCa                                                               >  2:1108673/1‑93 (MQ=255)

                            gAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCaa                                                              <  1:3050465/93‑1 (MQ=255)

                            gAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCaa                                                              <  1:5000065/93‑1 (MQ=255)

                             aGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAAt                                                             >  1:3401930/1‑93 (MQ=255)

                             aGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGGCAAt                                                             >  1:5994714/1‑93 (MQ=255)

                             aGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAAACAACTCGTCCAt                                                             >  1:3501444/1‑93 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGa                                                           >  1:2508974/1‑93 (MQ=255)

                               tGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGa                                                           <  2:608020/93‑1 (MQ=255)

                                 aTTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACt                                                         <  2:3559579/93‑1 (MQ=255)

                                 aTTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACt                                                         >  1:5882658/1‑93 (MQ=255)

                                  tttCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTg                                                        >  1:5825035/1‑93 (MQ=255)

                                  tttCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTg                                                        >  1:2433607/1‑93 (MQ=255)

                                   ttCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGc                                                       <  2:4631474/93‑1 (MQ=255)

                                    tcaccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGcc                                                      >  2:319107/1‑93 (MQ=255)

                                    tcaccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGcc                                                      >  2:2992057/1‑93 (MQ=255)

                                      accgtcaccgGCACGACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGCC‑C‑CCCCGCGCGGCCAATCACTTCGTCAATGACTGCCaa                                                    <  2:4076781/93‑1 (MQ=255)

                                      accgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCaa                                                    <  2:4023310/93‑1 (MQ=255)

                                       ccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAAc                                                   <  2:5133861/93‑1 (MQ=255)

                                       ccgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAAc                                                   >  1:1193615/1‑93 (MQ=255)

                                        cgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACg                                                  <  2:1940395/93‑1 (MQ=255)

                                        cgtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACg                                                  >  1:3743354/1‑93 (MQ=255)

                                         gtcaccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCTATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACgg                                                 <  1:2781094/93‑1 (MQ=255)

                                           caccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGa                                               <  1:385066/93‑1 (MQ=255)

                                             ccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt                                             >  2:3416505/1‑93 (MQ=255)

                                             ccgGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCCCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt                                             >  2:3431534/1‑93 (MQ=255)

                                                   caACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTtgttgt                                       <  1:5473901/93‑1 (MQ=255)

                                                    aaCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTtgttgtg                                      >  2:1198288/1‑92 (MQ=255)

                                                    aaCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTa                                      <  1:1161156/93‑1 (MQ=255)

                                                    aaCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTa                                      <  1:1751113/93‑1 (MQ=255)

                                                     aCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAg                                     >  2:3663456/1‑93 (MQ=255)

                                                     aCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAg                                     >  1:1671782/1‑93 (MQ=255)

                                                     aCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAg                                     >  2:3618612/1‑93 (MQ=255)

                                                       cGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCt                                   >  2:2730300/1‑93 (MQ=255)

                                                          tCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgtg                                <  2:1772871/93‑1 (MQ=255)

                                                          tCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCtgtg                                <  2:3477342/93‑1 (MQ=255)

                                                           ccGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTgg                               <  2:3831530/93‑1 (MQ=255)

                                                           ccGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTgg                               <  2:1249927/93‑1 (MQ=255)

                                                            cGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTggg                              <  1:1587919/93‑1 (MQ=255)

                                                               cAAAAAGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGgcgc                           >  1:5589678/1‑93 (MQ=255)

                                                                aaaaaGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGca                          >  1:4888285/1‑93 (MQ=255)

                                                                aaaaaGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGca                          >  1:1299842/1‑93 (MQ=255)

                                                                aaaaaGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGca                          >  2:4776964/1‑93 (MQ=255)

                                                                aaaaaGAGGCGTACCAATGTAGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGca                          >  2:6119166/1‑93 (MQ=255)

                                                                 aaaaGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaa                         >  2:665553/1‑93 (MQ=255)

                                                                  aaaGAGGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGcaca                        >  1:1908977/1‑91 (MQ=255)

                                                                     gagGCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCaacaac                     >  2:1819206/1‑93 (MQ=255)

                                                                       ggCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACAt                   >  2:4308754/1‑93 (MQ=255)

                                                                        gCGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATg                  <  1:2064135/93‑1 (MQ=255)

                                                                         cGTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGc                 >  2:4826862/1‑93 (MQ=255)

                                                                          gTACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGcc                >  1:1574894/1‑93 (MQ=255)

                                                                           tACCAATGTCGATATAG‑TCC‑CCCCGCGCTGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGccc               <  2:5948179/93‑1 (MQ=255)

                                                                           tACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGccc               >  1:529829/1‑93 (MQ=255)

                                                                           tACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGccc               >  2:2291931/1‑93 (MQ=255)

                                                                           tACCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGATGTGGGCGCAACAACATGccc               >  2:1610845/1‑93 (MQ=255)

                                                                            tccAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCa              <  1:2118178/92‑1 (MQ=255)

                                                                            aCCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCa              >  1:4231566/1‑93 (MQ=255)

                                                                            aCCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCa              <  1:4718917/93‑1 (MQ=255)

                                                                            aCCAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCa              <  1:6247556/93‑1 (MQ=255)

                                                                              cAATGTCGATATAG‑TCC‑CCCCGCGCGGCCAATCACTTCGTCAATTGCTGCCCACGGGAGTTGTTCTAGCTGGGGCCGCAACATGATGCCCaaa            >  2:5092604/1‑91 (MQ=255)

                                                                              cAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            <  1:4471778/93‑1 (MQ=255)

                                                                              cAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            <  1:4178193/93‑1 (MQ=255)

                                                                              cAATGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCaga            <  1:5422432/93‑1 (MQ=255)

                                                                                aTGTCGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGc          <  1:5695128/93‑1 (MQ=255)

                                                                                    cGATATAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTa      <  1:4533707/93‑1 (MQ=255)

                                                                                      atatAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAcc    <  1:1518794/93‑1 (MQ=255)

                                                                                      atatAG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAcc    <  1:21523/93‑1 (MQ=255)

                                                                                        ataG‑TCC‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCaa  >  2:5864712/1‑93 (MQ=255)

GCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑C‑CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAA  >  NC_000913/2563488‑2563667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCC  >  NC_000913/2563494‑2563677

ACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CCCCCcg                                                                                            <  SRR4302533.2649217/94‑3 (MQ=60)

 CCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CCCCC‑CGC                                                                                          <  SRR4302533.1037897/94‑1 (MQ=60)

  CGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CCCCC‑CGCG                                                                                         >  SRR4302533.3138374/1‑94 (MQ=60)

  CGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CCCCC‑CGCG                                                                                         >  SRR4302533.4451142/1‑94 (MQ=60)

  CGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CCCCC‑CGCG                                                                                         <  SRR4302533.4749863/94‑1 (MQ=60)

    CATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCG                                                                                         <  SRR4302533.5536033/94‑1 (MQ=60)

     ATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGG                                                                                        <  SRR4302533.2004459/94‑1 (MQ=60)

         AGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAA                                                                                    >  SRR4302533.91914/1‑94 (MQ=60)

         AGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAA                                                                                    >  SRR4302533.39126/1‑94 (MQ=60)

          GGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAAT                                                                                   >  SRR4302533.3221527/1‑94 (MQ=60)

          GGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAAT                                                                                   <  SRR4302533.2799822/94‑1 (MQ=60)

          GGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAAT                                                                                   >  SRR4302533.2009510/1‑94 (MQ=60)

          GGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAAT                                                                                   <  SRR4302533.4494637/94‑1 (MQ=60)

           GAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATC                                                                                  >  SRR4302533.6145930/1‑94 (MQ=60)

             AGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCAC                                                                                <  SRR4302533.4101211/94‑1 (MQ=60)

              GGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACT                                                                               <  SRR4302533.935129/94‑1 (MQ=60)

               GAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTT                                                                              <  SRR4302533.836731/94‑1 (MQ=60)

               GAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TACGCCCGCGCGGACAATAACTT                                                                              >  SRR4302533.4257296/1‑94 (MQ=60)

                AAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTC                                                                             <  SRR4302533.5578247/94‑1 (MQ=60)

                AAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTC                                                                             <  SRR4302533.2496180/94‑1 (MQ=60)

                AAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTC                                                                             >  SRR4302533.2475017/1‑94 (MQ=60)

                 AATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCG                                                                            >  SRR4302533.2498882/1‑94 (MQ=60)

                 AATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCG                                                                            <  SRR4302533.4847785/94‑1 (MQ=60)

                 AATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCG                                                                            <  SRR4302533.500119/94‑1 (MQ=60)

                    GCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCA                                                                         <  SRR4302533.2977037/94‑1 (MQ=60)

                     CGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAA                                                                        >  SRR4302533.6050813/1‑94 (MQ=60)

                     CGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAA                                                                        >  SRR4302533.3333881/1‑94 (MQ=60)

                      GAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAAacaactcgtccat                                                                       >  SRR4302533.3523037/1‑81 (MQ=60)

                      GAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAAT                                                                       >  SRR4302533.3422936/1‑94 (MQ=60)

                      GAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAAT                                                                       <  SRR4302533.3069135/94‑1 (MQ=60)

                      GAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAAT                                                                       <  SRR4302533.5030374/94‑1 (MQ=60)

                      GAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGGCAAT                                                                       >  SRR4302533.6031022/1‑94 (MQ=60)

                       AGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATG                                                                      >  SRR4302533.4231802/1‑94 (MQ=60)

                       AGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATG                                                                      >  SRR4302533.5667164/1‑94 (MQ=60)

                        GTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGA                                                                     >  SRR4302533.2524432/1‑94 (MQ=60)

                         TGNTNTNANCGTCACCGGCACANCCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGAC                                                                    <  SRR4302533.4443000/94‑1 (MQ=60)

                         TGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGAC                                                                    >  SRR4302533.4534001/1‑94 (MQ=60)

                          GATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACT                                                                   <  SRR4302533.2053734/94‑1 (MQ=60)

                          GATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACT                                                                   >  SRR4302533.5918208/1‑94 (MQ=60)

                           ATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTG                                                                  >  SRR4302533.2448642/1‑94 (MQ=60)

                           ATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTG                                                                  >  SRR4302533.5860224/1‑94 (MQ=60)

                              TCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCA                                                               >  SRR4302533.4192939/1‑94 (MQ=60)

                                ACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAAC                                                             >  SRR4302533.1200789/1‑94 (MQ=60)

                                 CCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACG                                                            >  SRR4302533.3766313/1‑94 (MQ=60)

                                  CGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGG                                                           >  SRR4302533.5953974/1‑94 (MQ=60)

                                  CGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGG                                                           <  SRR4302533.6180018/94‑1 (MQ=60)

                                   GTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCTATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGG                                                          <  SRR4302533.2798178/94‑1 (MQ=60)

                                    TCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGA                                                         <  SRR4302533.1062757/94‑1 (MQ=60)

                                     CACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAG                                                        <  SRR4302533.387390/94‑1 (MQ=60)

                                     CACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAG                                                        >  SRR4302533.3770484/1‑94 (MQ=60)

                                      ACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGT                                                       >  SRR4302533.5624290/1‑94 (MQ=60)

                                      ACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGT                                                       <  SRR4302533.1341843/94‑1 (MQ=60)

                                        CGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTG                                                     <  SRR4302533.4128782/94‑1 (MQ=60)

                                           CACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTG                                                  >  SRR4302533.941433/1‑94 (MQ=60)

                                           CACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTG                                                  <  SRR4302533.1637997/94‑1 (MQ=60)

                                             CAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTA                                                >  SRR4302533.1238278/1‑94 (MQ=60)

                                             CAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTA                                                <  SRR4302533.5506894/94‑1 (MQ=60)

                                              AACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAG                                               >  SRR4302533.5839111/1‑94 (MQ=60)

                                              AACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAG                                               <  SRR4302533.1168124/94‑1 (MQ=60)

                                              AACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAG                                               >  SRR4302533.1681887/1‑94 (MQ=60)

                                              AACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAG                                               <  SRR4302533.1761775/94‑1 (MQ=60)

                                              AACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAG                                               >  SRR4302533.185089/1‑94 (MQ=60)

                                                CCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCT                                             >  SRR4302533.5905995/1‑94 (MQ=60)

                                                CCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCT                                             <  SRR4302533.4783209/94‑1 (MQ=60)

                                                 CGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTG                                            >  SRR4302533.6069706/1‑94 (MQ=60)

                                                 CGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTG                                            >  SRR4302533.5501016/1‑94 (MQ=60)

                                                 CGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTG                                            >  SRR4302533.4884004/1‑94 (MQ=60)

                                                   ATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTG                                          <  SRR4302533.3211210/94‑1 (MQ=60)

                                                     CCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGG                                        <  SRR4302533.2125070/94‑1 (MQ=60)

                                                      CGCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGC                                       <  SRR4302533.1597506/94‑1 (MQ=60)

                                                       GCCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCG                                      >  SRR4302533.4749298/1‑94 (MQ=60)

                                                        CCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGC                                     >  SRR4302533.5623446/1‑94 (MQ=60)

                                                        CCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGC                                     >  SRR4302533.1171571/1‑94 (MQ=60)

                                                        CCAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGC                                     >  SRR4302533.2556254/1‑94 (MQ=60)

                                                         CAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCA                                    >  SRR4302533.4917902/1‑94 (MQ=60)

                                                         CAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCA                                    >  SRR4302533.1307663/1‑94 (MQ=60)

                                                         CAAAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGATGTTGTAGCTGTGGGCGCA                                    >  SRR4302533.2745823/1‑94 (MQ=60)

                                                           AAAAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAca                                  >  SRR4302533.1920622/1‑92 (MQ=60)

                                                             AAGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAA                                >  SRR4302533.5076258/1‑94 (MQ=60)

                                                              AGAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAAC                               <  SRR4302533.2247532/94‑1 (MQ=60)

                                                               GAGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACA                              <  SRR4302533.1448923/94‑1 (MQ=60)

                                                                AGGCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACAT                             <  SRR4302533.1686129/94‑1 (MQ=60)

                                                                AGGCGTACCAAGGTCGATATAGAC‑CCCCCGCGCGGTCAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACAT                             <  SRR4302533.3672847/94‑1 (MQ=60)

                                                                  GCGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGC                           <  SRR4302533.2076858/94‑1 (MQ=60)

                                                                   CGTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCC                          >  SRR4302533.1584380/1‑94 (MQ=60)

                                                                    GTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCC                         >  SRR4302533.532981/1‑94 (MQ=60)

                                                                    GTACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCC                         <  SRR4302533.2520206/94‑1 (MQ=60)

                                                                     TACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCA                        >  SRR4302533.4257142/1‑94 (MQ=60)

                                                                     TACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCA                        <  SRR4302533.3576035/94‑1 (MQ=60)

                                                                      TCCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAG                       <  SRR4302533.2131240/94‑1 (MQ=60)

                                                                      ACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAG                       <  SRR4302533.4747531/94‑1 (MQ=60)

                                                                      ACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAG                       <  SRR4302533.6284942/94‑1 (MQ=60)

                                                                      ACCAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAG                       <  SRR4302533.3156299/94‑1 (MQ=60)

                                                                        CAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAG                     <  SRR4302533.5455061/94‑1 (MQ=60)

                                                                        CAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAG                     <  SRR4302533.4498825/94‑1 (MQ=60)

                                                                        CAATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAG                     <  SRR4302533.4203505/94‑1 (MQ=60)

                                                                          ATGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCT                   <  SRR4302533.5729520/94‑1 (MQ=60)

                                                                           TGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTT                  <  SRR4302533.2047902/94‑1 (MQ=60)

                                                                           TGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTT                  <  SRR4302533.773357/94‑1 (MQ=60)

                                                                           TGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTT                  >  SRR4302533.4529530/1‑94 (MQ=60)

                                                                           TGTCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTT                  <  SRR4302533.1595919/94‑1 (MQ=60)

                                                                             TCGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTA                <  SRR4302533.1753220/94‑1 (MQ=60)

                                                                              CGATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTAC               <  SRR4302533.4561158/94‑1 (MQ=60)

                                                                               GATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACC              <  SRR4302533.5416948/94‑1 (MQ=60)

                                                                               GATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACC              >  SRR4302533.3618760/1‑94 (MQ=60)

                                                                               GATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACC              <  SRR4302533.4752674/94‑1 (MQ=60)

                                                                               GATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACC              >  SRR4302533.1555033/1‑94 (MQ=60)

                                                                                ATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCA             <  SRR4302533.21665/94‑1 (MQ=60)

                                                                                ATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCA             >  SRR4302533.3423463/1‑94 (MQ=60)

                                                                                ATATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCA             <  SRR4302533.1527936/94‑1 (MQ=60)

                                                                                 TATAG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAA            >  SRR4302533.3111558/1‑94 (MQ=60)

                                                                                    AG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGT         >  SRR4302533.2385967/1‑94 (MQ=60)

                                                                                    AG‑TCCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGT         <  SRR4302533.4558981/94‑1 (MQ=60)

                                                                                       C‑CCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCT      <  SRR4302533.5051996/94‑1 (MQ=60)

                                                                                       C‑CCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCT      <  SRR4302533.3253880/94‑1 (MQ=60)

                                                                                         CCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTG     >  SRR4302533.5683947/1‑94 (MQ=60)

                                                                                         CCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTG     <  SRR4302533.1717119/94‑1 (MQ=60)

                                                                                         CCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTG     <  SRR4302533.2630108/94‑1 (MQ=60)

                                                                                          CCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGC    >  SRR4302533.5844077/1‑94 (MQ=60)

                                                                                            CCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCC  >  SRR4302533.4823218/1‑94 (MQ=60)

ACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAG‑T‑CCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCC  >  NC_000913/2563494‑2563677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`