Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,585,577 G→A intergenic (+126/‑38) narQ → / → acrD NarQ/AcrD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,585,5770GA99.2% 842.0 / NA 234intergenic (+126/‑38)narQ/acrDNarQ/AcrD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (118/114);  minor base C (0/2);  total (118/116)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

GTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAGCCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCC  >  NC_000913/2585487‑2585666
                                                                                          |                                                                                         
 ttCAGTATGAAAGGGCATAATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCt                                                                                        <  1:4098957/93‑1 (MQ=255)
 ttCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCt                                                                                        >  1:3830118/1‑93 (MQ=255)
  tCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTa                                                                                       <  2:1977844/93‑1 (MQ=255)
  tCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAACAAGTCAAACCTa                                                                                       >  2:5137569/1‑93 (MQ=255)
    aGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACa                                                                                     <  1:2409930/93‑1 (MQ=255)
    aGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACa                                                                                     >  1:352831/1‑93 (MQ=255)
    aGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACa                                                                                     <  1:5315579/93‑1 (MQ=255)
     gTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACaa                                                                                    >  1:5113197/1‑93 (MQ=255)
      tataAAAGGGCATGATAATTTACATTACCTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAAc                                                                                   <  1:3856532/93‑1 (MQ=255)
      tataAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAAc                                                                                   <  2:184076/93‑1 (MQ=255)
      tataAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAAc                                                                                   >  1:2085917/1‑93 (MQ=255)
       ataAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACg                                                                                  <  2:3558129/93‑1 (MQ=255)
         aaaaGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGAt                                                                                <  2:4877303/93‑1 (MQ=255)
          aaaGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATa                                                                               <  1:6013002/93‑1 (MQ=255)
           aaGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATAc                                                                              <  2:3878293/93‑1 (MQ=255)
               gCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTATAACGATACGCAg                                                                          <  2:3836042/93‑1 (MQ=255)
               gCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAg                                                                          >  1:3398866/1‑93 (MQ=255)
               gCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAg                                                                          <  2:3439555/93‑1 (MQ=255)
               gCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAg                                                                          >  2:1428404/1‑93 (MQ=255)
               gCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAg                                                                          >  1:4844390/1‑93 (MQ=255)
                 aTGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGaa                                                                        >  1:5404465/1‑93 (MQ=255)
                  tGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCCAACCTACAACGATACGCAGaaa                                                                       >  1:4667536/1‑93 (MQ=255)
                    aTAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAaca                                                                     <  1:284787/93‑1 (MQ=255)
                    aTAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACCCAGAAaca                                                                     >  2:4345724/1‑93 (MQ=255)
                      aaTTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACg                                                                   <  2:1570972/93‑1 (MQ=255)
                         ttACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAgg                                                                >  2:548533/1‑93 (MQ=255)
                            cATTAACTCCTTTTTTTCTCCACGATTGGCTCGTCCCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTcc                                                             <  1:1905113/93‑1 (MQ=255)
                             aTTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCct                                                            <  1:1448821/93‑1 (MQ=255)
                             aTTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCct                                                            >  1:1096857/1‑93 (MQ=255)
                              ttAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCctc                                                           <  2:4166374/93‑1 (MQ=255)
                              ttAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCctc                                                           >  2:646472/1‑93 (MQ=255)
                               tAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCctct                                                          <  1:827815/93‑1 (MQ=255)
                               tAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCctct                                                          <  1:1692508/93‑1 (MQ=255)
                                aaCTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCtt                                                         <  1:4044391/93‑1 (MQ=255)
                                 aCTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCttt                                                        <  1:1160899/93‑1 (MQ=255)
                                   tCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTa                                                      >  2:1344257/1‑93 (MQ=255)
                                    ccTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTaa                                                     >  2:3344235/1‑93 (MQ=255)
                                    ccTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTaa                                                     >  2:4147715/1‑93 (MQ=255)
                                    ccTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTaa                                                     <  1:5761862/93‑1 (MQ=255)
                                     cTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAt                                                    >  1:4377949/1‑93 (MQ=255)
                                     cTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAt                                                    >  1:847927/1‑93 (MQ=255)
                                     cTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGCGCTCTTTTAAt                                                    >  1:1186646/1‑93 (MQ=255)
                                      tttttttCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATg                                                   >  2:3127359/1‑93 (MQ=255)
                                      tttttttCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATg                                                   <  1:4752931/93‑1 (MQ=255)
                                      tttttttCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATg                                                   <  1:5984471/93‑1 (MQ=255)
                                        tttttCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGc                                                 <  2:3154780/93‑1 (MQ=255)
                                         ttttCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCg                                                <  2:4035803/93‑1 (MQ=255)
                                          tttCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGa                                               >  2:836059/1‑93 (MQ=255)
                                           ttCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGaa                                              <  2:2024867/93‑1 (MQ=255)
                                             ctcCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAAtt                                            >  1:4495368/1‑93 (MQ=255)
                                              tcCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAAttt                                           >  2:2230949/1‑93 (MQ=255)
                                               ccACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAAtttt                                          >  1:3961115/1‑92 (MQ=255)
                                               ccACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTc                                          >  1:5270005/1‑93 (MQ=255)
                                                cACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCt                                         >  1:1228146/1‑93 (MQ=255)
                                                cACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCt                                         <  2:104993/93‑1 (MQ=255)
                                                 aCGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCtt                                        >  2:5015686/1‑93 (MQ=255)
                                                  cGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCttt                                       >  1:3595434/1‑93 (MQ=255)
                                                   gATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAATCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTa                                      <  2:1236048/93‑1 (MQ=255)
                                                   gATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCtttt                                      >  2:1767952/1‑92 (MQ=255)
                                                    aTTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTAt                                     <  2:3030508/93‑1 (MQ=255)
                                                    aTTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTAt                                     <  2:1895738/93‑1 (MQ=255)
                                                    aTTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTAt                                     >  1:4554594/1‑93 (MQ=255)
                                                     ttGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAACCCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTAtt                                    <  2:4715376/93‑1 (MQ=255)
                                                     ttGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTAtt                                    >  1:2256074/1‑93 (MQ=255)
                                                     ttGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTAtt                                    <  1:4226715/93‑1 (MQ=255)
                                                       ggCTCGTACCTTTTCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGCTCCTCTTTTAATGGCGAATTTCTTTTTTGa                                  >  2:4285484/1‑93 (MQ=255)
                                                       ggCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGa                                  <  1:3509/93‑1 (MQ=255)
                                                       ggCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGa                                  <  1:3912410/93‑1 (MQ=255)
                                                       ggCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGa                                  <  1:4068723/93‑1 (MQ=255)
                                                         cTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATc                                <  1:2522131/93‑1 (MQ=255)
                                                         cTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATc                                <  1:4985967/93‑1 (MQ=255)
                                                         cTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATc                                >  1:4466961/1‑93 (MQ=255)
                                                          tCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCg                               >  2:2937294/1‑93 (MQ=255)
                                                           cGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGc                              >  2:2402812/1‑93 (MQ=255)
                                                           cGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGc                              >  1:3147815/1‑93 (MQ=255)
                                                           cGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGc                              <  2:48563/93‑1 (MQ=255)
                                                            gTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGcc                             >  2:1296956/1‑93 (MQ=255)
                                                            gTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGcc                             >  1:3576271/1‑93 (MQ=255)
                                                             tACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGccc                            >  1:2996100/1‑93 (MQ=255)
                                                             tACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGccc                            <  2:3682779/93‑1 (MQ=255)
                                                              aCCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGcccc                           <  2:2963528/93‑1 (MQ=255)
                                                               ccTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGCTCGCCCCa                          >  2:5890073/1‑93 (MQ=255)
                                                               ccTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCa                          <  1:5588188/93‑1 (MQ=255)
                                                                cTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAt                         <  2:2100987/93‑1 (MQ=255)
                                                                 ttGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAtt                        <  2:5219597/93‑1 (MQ=255)
                                                                 ttGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAtt                        >  2:616823/1‑93 (MQ=255)
                                                                 ttGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAtt                        >  2:3262982/1‑93 (MQ=255)
                                                                  tGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAttt                       <  1:921602/93‑1 (MQ=255)
                                                                   gCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAtttt                      <  1:2717661/93‑1 (MQ=255)
                                                                    ccGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAttttt                     >  2:2507512/1‑93 (MQ=255)
                                                                      gCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGc                   >  2:5592780/1‑93 (MQ=255)
                                                                        tACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCt                 >  1:3244549/1‑93 (MQ=255)
                                                                        tACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCt                 >  2:2162718/1‑93 (MQ=255)
                                                                         aCAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTg                <  2:4411679/93‑1 (MQ=255)
                                                                         aCAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTg                >  1:727699/1‑93 (MQ=255)
                                                                         aCAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTg                <  2:2897520/93‑1 (MQ=255)
                                                                         aCAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTg                >  2:2743225/1‑93 (MQ=255)
                                                                           aGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTggg              <  2:2656844/93‑1 (MQ=255)
                                                                                       cAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATcc  <  2:5926234/93‑1 (MQ=255)
                                                                                       cAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATcc  <  1:5792214/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAGCCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCC  >  NC_000913/2585487‑2585666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAGCCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGT  >  NC_000913/2585485‑2585669
                                                                                            |                                                                                            
AGGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAAC                                                                                             >  SRR4302533.57329/1‑94 (MQ=60)
 GGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTATCTTGCCGCTACAGTGAAGCAAGTCAAACC                                                                                            <  SRR4302533.3980834/94‑1 (MQ=60)
 GGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACC                                                                                            <  SRR4302533.4868631/94‑1 (MQ=60)
 GGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACC                                                                                            <  SRR4302533.4868470/94‑1 (MQ=60)
 GGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACC                                                                                            <  SRR4302533.1039321/94‑1 (MQ=60)
 GGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACC                                                                                            >  SRR4302533.3568636/1‑94 (MQ=60)
  GTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCT                                                                                           >  SRR4302533.3853533/1‑94 (MQ=60)
  GTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCT                                                                                           <  SRR4302533.5673966/94‑1 (MQ=60)
   TTCAGTATGAAAGGGCATAATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTA                                                                                          <  SRR4302533.4123906/94‑1 (MQ=60)
     CAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACA                                                                                        >  SRR4302533.354982/1‑94 (MQ=60)
      AGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAA                                                                                       >  SRR4302533.5144095/1‑94 (MQ=60)
      AGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAA                                                                                       <  SRR4302533.2424814/94‑1 (MQ=60)
      AGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAA                                                                                       <  SRR4302533.5347433/94‑1 (MQ=60)
       GTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAAC                                                                                      >  SRR4302533.2098763/1‑94 (MQ=60)
       GTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAAC                                                                                      <  SRR4302533.4208114/94‑1 (MQ=60)
        TATAAAAGGGCATGATAATTTACATTACCTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACG                                                                                     <  SRR4302533.3880077/94‑1 (MQ=60)
            AAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATAC                                                                                 <  SRR4302533.91122/94‑1 (MQ=60)
            AAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATAC                                                                                 <  SRR4302533.6049431/94‑1 (MQ=60)
             AAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACG                                                                                <  SRR4302533.3120144/94‑1 (MQ=60)
              AGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGC                                                                               <  SRR4302533.4424891/94‑1 (MQ=60)
                GGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAG                                                                             <  SRR4302533.6046364/94‑1 (MQ=60)
                GGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAG                                                                             >  SRR4302533.4873753/1‑94 (MQ=60)
                GGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAG                                                                             >  SRR4302533.3419855/1‑94 (MQ=60)
                  CATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGGA                                                                           >  SRR4302533.3245157/1‑94 (MQ=60)
                  CATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAA                                                                           >  SRR4302533.5436942/1‑94 (MQ=60)
                   ATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCCAACCTACAACGATACGCAGAAA                                                                          >  SRR4302533.4695815/1‑94 (MQ=60)
                    TGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAAC                                                                         >  SRR4302533.1132405/1‑94 (MQ=60)
                      ATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACAC                                                                       <  SRR4302533.286527/94‑1 (MQ=60)
                        AATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGA                                                                     >  SRR4302533.118985/1‑94 (MQ=60)
                         ATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAG                                                                    <  SRR4302533.626487/94‑1 (MQ=60)
                           TTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAAAACGAGGC                                                                  >  SRR4302533.4972353/1‑94 (MQ=60)
                              CATTAGCTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCT                                                               <  SRR4302533.1666728/94‑1 (MQ=60)
                              CATTAACTCCTTTTTTTCTCCACGATTGGCTCGTCCCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCT                                                               <  SRR4302533.1916736/94‑1 (MQ=60)
                              CATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCT                                                               >  SRR4302533.1103354/1‑94 (MQ=60)
                              CATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCT                                                               >  SRR4302533.2440629/1‑94 (MQ=60)
                              CATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCT                                                               <  SRR4302533.5116226/94‑1 (MQ=60)
                               ATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTC                                                              <  SRR4302533.1457562/94‑1 (MQ=60)
                               ATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTC                                                              >  SRR4302533.3863716/1‑94 (MQ=60)
                                TTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCT                                                             >  SRR4302533.846432/1‑94 (MQ=60)
                                 TAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTT                                                            <  SRR4302533.832671/94‑1 (MQ=60)
                                 TAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTT                                                            <  SRR4302533.4186923/94‑1 (MQ=60)
                                 TAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTT                                                            <  SRR4302533.3600327/94‑1 (MQ=60)
                                 TAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTT                                                            <  SRR4302533.1702761/94‑1 (MQ=60)
                                  AGCTCCTTTTGTTCTCCACGATTGGCTCGTACCTTGCCGCTGCAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTT                                                           <  SRR4302533.2226444/94‑1 (MQ=60)
                                  AACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTT                                                           >  SRR4302533.4516029/1‑94 (MQ=60)
                                  AACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTT                                                           <  SRR4302533.4069007/94‑1 (MQ=60)
                                   ACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTT                                                          <  SRR4302533.1167866/94‑1 (MQ=60)
                                    CTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTA                                                         <  SRR4302533.351578/94‑1 (MQ=60)
                                    CTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTA                                                         >  SRR4302533.179574/1‑94 (MQ=60)
                                     TCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAA                                                        >  SRR4302533.5223690/1‑94 (MQ=60)
                                     TCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAA                                                        >  SRR4302533.4756015/1‑94 (MQ=60)
                                     TCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAA                                                        <  SRR4302533.1961544/94‑1 (MQ=60)
                                      CCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAT                                                       >  SRR4302533.3933499/1‑94 (MQ=60)
                                      CCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAT                                                       >  SRR4302533.4404381/1‑94 (MQ=60)
                                      CCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAT                                                       <  SRR4302533.5796635/94‑1 (MQ=60)
                                      CCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAT                                                       >  SRR4302533.852889/1‑94 (MQ=60)
                                      CCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAT                                                       <  SRR4302533.830889/94‑1 (MQ=60)
                                      CCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAAT                                                       <  SRR4302533.3589077/94‑1 (MQ=60)
                                      CCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGCGCTCTTTTAAT                                                       >  SRR4302533.1193767/1‑94 (MQ=60)
                                       CTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATG                                                      <  SRR4302533.2833132/94‑1 (MQ=60)
                                       CTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATG                                                      <  SRR4302533.5831119/94‑1 (MQ=60)
                                        TTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGG                                                     <  SRR4302533.6020714/94‑1 (MQ=60)
                                        TTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGG                                                     <  SRR4302533.4781745/94‑1 (MQ=60)
                                           TTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGA                                                  <  SRR4302533.2658313/94‑1 (MQ=60)
                                              TCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATT                                               <  SRR4302533.2600922/94‑1 (MQ=60)
                                              TCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATT                                               >  SRR4302533.4522590/1‑94 (MQ=60)
                                              TCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATT                                               <  SRR4302533.3871519/94‑1 (MQ=60)
                                               CTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTT                                              <  SRR4302533.2407906/94‑1 (MQ=60)
                                               CTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTT                                              >  SRR4302533.5407271/1‑94 (MQ=60)
                                               CTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTT                                              >  SRR4302533.486845/1‑94 (MQ=60)
                                                TCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTT                                             >  SRR4302533.3985248/1‑94 (MQ=60)
                                                TCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTC                                             >  SRR4302533.5301611/1‑94 (MQ=60)
                                                TCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTC                                             <  SRR4302533.3097218/94‑1 (MQ=60)
                                                 CCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCT                                            >  SRR4302533.1235499/1‑94 (MQ=60)
                                                  CACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTT                                           >  SRR4302533.143744/1‑94 (MQ=60)
                                                   ACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTT                                          >  SRR4302533.656646/1‑94 (MQ=60)
                                                   ACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTT                                          <  SRR4302533.5590974/94‑1 (MQ=60)
                                                   ACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTT                                          >  SRR4302533.3617555/1‑94 (MQ=60)
                                                     GATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTAT                                        >  SRR4302533.4582215/1‑94 (MQ=60)
                                                      ATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATT                                       >  SRR4302533.2270032/1‑94 (MQ=60)
                                                       TTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTG                                      <  SRR4302533.487382/94‑1 (MQ=60)
                                                       TTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTG                                      <  SRR4302533.121437/94‑1 (MQ=60)
                                                       TTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTG                                      <  SRR4302533.4252274/94‑1 (MQ=60)
                                                        TGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGA                                     >  SRR4302533.2963948/1‑94 (MQ=60)
                                                         GGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGAT                                    <  SRR4302533.3538/94‑1 (MQ=60)
                                                         GGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGAT                                    >  SRR4302533.4648122/1‑94 (MQ=60)
                                                         GGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGAT                                    <  SRR4302533.3936303/94‑1 (MQ=60)
                                                         GGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGAT                                    <  SRR4302533.3823342/94‑1 (MQ=60)
                                                         GGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGAT                                    <  SRR4302533.4283038/94‑1 (MQ=60)
                                                         GGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGAT                                    <  SRR4302533.4093491/94‑1 (MQ=60)
                                                          GCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATC                                   >  SRR4302533.4493976/1‑94 (MQ=60)
                                                          GCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATC                                   <  SRR4302533.4077420/94‑1 (MQ=60)
                                                           CTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCG                                  <  SRR4302533.5016199/94‑1 (MQ=60)
                                                           CTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCG                                  <  SRR4302533.4482928/94‑1 (MQ=60)
                                                           CTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCG                                  <  SRR4302533.2537672/94‑1 (MQ=60)
                                                           CTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCG                                  >  SRR4302533.3938260/1‑94 (MQ=60)
                                                           CTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCG                                  >  SRR4302533.4537157/1‑94 (MQ=60)
                                                           CTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCG                                  >  SRR4302533.3265282/1‑94 (MQ=60)
                                                            TCGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGC                                 >  SRR4302533.3167168/1‑94 (MQ=60)
                                                             CGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCC                                >  SRR4302533.3598312/1‑94 (MQ=60)
                                                             CGTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCC                                >  SRR4302533.5592691/1‑94 (MQ=60)
                                                              GTACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCC                               >  SRR4302533.3014421/1‑94 (MQ=60)
                                                                ACCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCA                             >  SRR4302533.1789251/1‑94 (MQ=60)
                                                                 CCTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCAT                            <  SRR4302533.5621950/94‑1 (MQ=60)
                                                                  CTTGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATT                           <  SRR4302533.940778/94‑1 (MQ=60)
                                                                    TGCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTT                         <  SRR4302533.927012/94‑1 (MQ=60)
                                                                     GCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTT                        <  SRR4302533.2420821/94‑1 (MQ=60)
                                                                     GCCGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTT                        <  SRR4302533.2734347/94‑1 (MQ=60)
                                                                       CGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGC                      >  SRR4302533.5157040/1‑94 (MQ=60)
                                                                       CGCTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGC                      >  SRR4302533.1206971/1‑94 (MQ=60)
                                                                         CTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCT                    >  SRR4302533.5894544/1‑94 (MQ=60)
                                                                         CTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCT                    >  SRR4302533.3264549/1‑94 (MQ=60)
                                                                         CTACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCT                    <  SRR4302533.865616/94‑1 (MQ=60)
                                                                          TACAGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTG                   >  SRR4302533.731997/1‑94 (MQ=60)
                                                                             AGTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGT                <  SRR4302533.4033302/94‑1 (MQ=60)
                                                                              GTGAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTG               >  SRR4302533.3431269/1‑94 (MQ=60)
                                                                                GAAGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCT             <  SRR4302533.5305691/94‑1 (MQ=60)
                                                                                  AGCAAGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGG           <  SRR4302533.4045114/94‑1 (MQ=60)
                                                                                      AGTCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAAT       >  SRR4302533.4100051/1‑94 (MQ=60)
                                                                                        TCAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCC     >  SRR4302533.4583514/1‑94 (MQ=60)
                                                                                         CAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCT    <  SRR4302533.3536566/94‑1 (MQ=60)
                                                                                         CAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCT    <  SRR4302533.5827205/94‑1 (MQ=60)
                                                                                         CAAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCT    <  SRR4302533.5821882/94‑1 (MQ=60)
                                                                                          AAACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTG   <  SRR4302533.3323896/94‑1 (MQ=60)
                                                                                           AACCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGT  >  SRR4302533.3005888/1‑94 (MQ=60)
                                                                                            |                                                                                            
AGGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTTCTCCACGATTGGCTCGTACCTTGCCGCTACAGTGAAGCAAGTCAAGCCTACAACGATACGCAGAAACACGAGGTCCTCTTTTAATGGCGAATTTCTTTATTGATCGCCCCATTTTTGCCTGGGTGCTGGCAATCCTGT  >  NC_000913/2585485‑2585669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑