Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,590,989 T→C intergenic (+7/+103) b4537 → / ← ypfH hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,590,9890TC100.0% 801.8 / NA 250intergenic (+7/+103)b4537/ypfHhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (132/118);  total (132/118)

Only 100 of 259 total aligned reads displayed.

CCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCA  >  NC_000913/2590899‑2591078
                                                                                          |                                                                                         
 cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCt                                                                                        >  1:121856/1‑93 (MQ=255)
 cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCt                                                                                        >  2:3794583/1‑93 (MQ=255)
 cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCt                                                                                        <  2:4219234/93‑1 (MQ=255)
  ggAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTc                                                                                       >  1:3443562/1‑93 (MQ=255)
  ggAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTc                                                                                       >  2:4976158/1‑93 (MQ=255)
    aGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCaa                                                                                     <  2:449026/93‑1 (MQ=255)
     gCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAAc                                                                                    <  2:5691537/93‑1 (MQ=255)
       ttCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCg                                                                                  <  1:4158490/93‑1 (MQ=255)
       ttCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCg                                                                                  <  2:1936713/93‑1 (MQ=255)
       ttCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCg                                                                                  >  1:4915672/1‑93 (MQ=255)
        tCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGt                                                                                 >  1:302378/1‑93 (MQ=255)
         cccccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTa                                                                                <  1:3528536/93‑1 (MQ=255)
          ccccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAg                                                                               <  1:502332/93‑1 (MQ=255)
          ccccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAg                                                                               >  1:2768615/1‑93 (MQ=255)
          ccccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAg                                                                               >  2:5922851/1‑93 (MQ=255)
           cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAgg                                                                              <  1:1749032/93‑1 (MQ=255)
           cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAgg                                                                              >  2:5884251/1‑93 (MQ=255)
            ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGc                                                                             <  2:1213085/93‑1 (MQ=255)
            ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGc                                                                             >  2:3404748/1‑93 (MQ=255)
             cGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGcc                                                                            <  1:6225992/93‑1 (MQ=255)
              gCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCg                                                                           <  1:5972879/93‑1 (MQ=255)
              gCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCg                                                                           >  2:1861851/1‑93 (MQ=255)
              gCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCg                                                                           <  1:4738339/93‑1 (MQ=255)
              gCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCg                                                                           >  2:672598/1‑93 (MQ=255)
                aTCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCggt                                                                         >  1:5259606/1‑92 (MQ=255)
                aTCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGa                                                                         >  2:259150/1‑93 (MQ=255)
                aTCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGa                                                                         >  2:5223307/1‑93 (MQ=255)
                 tCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGACGGAt                                                                        >  1:139325/1‑93 (MQ=255)
                   gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATaa                                                                      >  1:549973/1‑93 (MQ=255)
                   gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATaa                                                                      <  2:784913/93‑1 (MQ=255)
                   gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATaa                                                                      <  1:3920664/93‑1 (MQ=255)
                    tGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAg                                                                     <  2:4616575/93‑1 (MQ=255)
                     gATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAgg                                                                    <  1:3129430/93‑1 (MQ=255)
                      aTTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGTGCGCTCAACCGTAGGCCGGATAAGGc                                                                   >  2:5093589/1‑93 (MQ=255)
                         tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGtt                                                                <  2:2308497/93‑1 (MQ=255)
                         tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGtt                                                                >  1:3660328/1‑93 (MQ=255)
                         tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGtt                                                                <  2:3181091/93‑1 (MQ=255)
                         tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGtt                                                                >  1:4848471/1‑93 (MQ=255)
                          cAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTa                                                               <  1:670903/93‑1 (MQ=255)
                           aaCGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTaa                                                              >  1:847801/1‑93 (MQ=255)
                           aaCGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTaa                                                              <  2:5395265/93‑1 (MQ=255)
                           aaCGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTaa                                                              >  1:75771/1‑93 (MQ=255)
                              gATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAAcgc                                                           >  2:3968274/1‑93 (MQ=255)
                               aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAAcgcc                                                          <  1:5794571/93‑1 (MQ=255)
                               aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAAcgcc                                                          >  2:4061578/1‑93 (MQ=255)
                                tAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAAcgccg                                                         >  1:4797396/1‑93 (MQ=255)
                                   gTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCAt                                                      >  2:549504/1‑93 (MQ=255)
                                    tGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc                                                     <  2:1320703/93‑1 (MQ=255)
                                      ggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCg                                                   <  2:1424944/93‑1 (MQ=255)
                                       gACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCgg                                                  >  1:3734866/1‑93 (MQ=255)
                                        aCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                 >  1:331182/1‑93 (MQ=255)
                                          gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCaa                                               >  1:3400435/1‑93 (MQ=255)
                                           aTGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                              >  2:5871595/1‑93 (MQ=255)
                                           aTGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                              <  2:2075701/93‑1 (MQ=255)
                                           aTGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                              <  2:4454185/93‑1 (MQ=255)
                                           aTGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                              >  2:5593566/1‑93 (MQ=255)
                                           aTGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                              >  1:191428/1‑93 (MQ=255)
                                           aTGACGACGACTGGCCGAAAAACGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                              <  1:201984/93‑1 (MQ=255)
                                            tGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATg                                             >  2:3931623/1‑93 (MQ=255)
                                             gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg                                            >  1:238947/1‑93 (MQ=255)
                                              acgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGt                                           >  1:2360203/1‑93 (MQ=255)
                                                 acgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGaa                                        >  1:2610101/1‑93 (MQ=255)
                                                 acgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGaa                                        >  1:1159983/1‑93 (MQ=255)
                                                 acgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGaa                                        >  1:5728157/1‑93 (MQ=255)
                                                  cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                       <  2:6074893/93‑1 (MQ=255)
                                                  cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                       >  1:161922/1‑93 (MQ=255)
                                                  cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                       <  1:2118293/93‑1 (MQ=255)
                                                    acTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGa                                     <  2:4967838/93‑1 (MQ=255)
                                                     cTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGAt                                    <  2:1568231/93‑1 (MQ=255)
                                                        gCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGcc                                 <  1:5366334/93‑1 (MQ=255)
                                                        gCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGcc                                 >  1:4553119/1‑93 (MQ=255)
                                                         ccGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt                                <  1:1044821/93‑1 (MQ=255)
                                                          cGAAAAAGGATCAACCAAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTg                               <  1:6154716/93‑1 (MQ=255)
                                                           gAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGa                              <  1:4199860/93‑1 (MQ=255)
                                                           gAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGa                              <  2:2900333/93‑1 (MQ=255)
                                                           gAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGA‑AAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGAt                             <  1:4075093/93‑1 (MQ=255)
                                                            aaaaaGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGAt                             >  1:1550797/1‑93 (MQ=255)
                                                            aaaaaGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGAt                             <  2:2207937/93‑1 (MQ=255)
                                                               aaGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCg                          >  2:2818601/1‑93 (MQ=255)
                                                                 ggATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGAc                        <  2:5199423/93‑1 (MQ=255)
                                                                 ggATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGAc                        <  2:5358232/93‑1 (MQ=255)
                                                                  gATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACg                       >  2:5974483/1‑93 (MQ=255)
                                                                   aTCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGc                      >  1:4426089/1‑93 (MQ=255)
                                                                    tCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCt                     >  2:3118809/1‑93 (MQ=255)
                                                                       aCCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAc                  <  2:474990/93‑1 (MQ=255)
                                                                         cGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcgc                >  1:4684767/1‑93 (MQ=255)
                                                                          gaagaaGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcgcg               >  1:3223885/1‑93 (MQ=255)
                                                                           aagaagTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGt              <  1:959408/93‑1 (MQ=255)
                                                                           aagaagTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGt              >  1:656595/1‑93 (MQ=255)
                                                                            agaagTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTc             <  1:2835152/93‑1 (MQ=255)
                                                                             gaagTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCt            <  1:1723432/93‑1 (MQ=255)
                                                                              aagTATGCTGAGCTCGCAACCGTAGACCGGATATGCCGTCAGCGCCGCAGGCGGCAATGGTGAGCGATGCCTGATGCGGCGCTCACGCGTCtt           <  2:3070504/93‑1 (MQ=16)
                                                                              aagTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCtt           >  2:4829713/1‑93 (MQ=255)
                                                                                gTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt         >  1:518196/1‑93 (MQ=255)
                                                                                gTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt         <  2:3492357/93‑1 (MQ=255)
                                                                                    gCTGAGCGCTCATCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg     <  1:3863794/93‑1 (MQ=255)
                                                                                     cTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGc    <  1:3254351/93‑1 (MQ=255)
                                                                                      tGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGcc   >  2:5243613/1‑93 (MQ=255)
                                                                                       gAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCa  <  2:4688657/93‑1 (MQ=255)
                                                                                       gAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCa  >  2:6197797/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCA  >  NC_000913/2590899‑2591078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGG  >  NC_000913/2590899‑2591080
                                                                                          |                                                                                           
CCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCT                                                                                          >  SRR4302533.1839388/1‑94 (MQ=60)
CCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCT                                                                                          >  SRR4302533.122623/1‑94 (MQ=60)
CCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCT                                                                                          <  SRR4302533.4349105/94‑1 (MQ=60)
 CGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTC                                                                                         >  SRR4302533.3464831/1‑94 (MQ=60)
   GAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAA                                                                                       >  SRR4302533.4880305/1‑94 (MQ=60)
   GAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAA                                                                                       <  SRR4302533.3989358/94‑1 (MQ=60)
      CTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCG                                                                                    >  SRR4302533.326291/1‑94 (MQ=60)
      CTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCG                                                                                    >  SRR4302533.4945448/1‑94 (MQ=60)
       TTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGT                                                                                   >  SRR4302533.304227/1‑94 (MQ=60)
       TTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGT                                                                                   <  SRR4302533.4183689/94‑1 (MQ=60)
        TCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTA                                                                                  >  SRR4302533.5804837/1‑94 (MQ=60)
         CCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAG                                                                                 <  SRR4302533.771811/94‑1 (MQ=60)
         CCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAG                                                                                 >  SRR4302533.2785603/1‑94 (MQ=60)
         CCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAG                                                                                 <  SRR4302533.3550284/94‑1 (MQ=60)
         CCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAG                                                                                 >  SRR4302533.2785604/1‑94 (MQ=60)
          CCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGG                                                                                >  SRR4302533.3451331/1‑94 (MQ=60)
          CCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGG                                                                                >  SRR4302533.672863/1‑94 (MQ=60)
          CCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGG                                                                                <  SRR4302533.505317/94‑1 (MQ=60)
           CCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGC                                                                               <  SRR4302533.1759681/94‑1 (MQ=60)
             CGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCG                                                                             <  SRR4302533.924504/94‑1 (MQ=60)
             CGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCG                                                                             <  SRR4302533.6263294/94‑1 (MQ=60)
              GCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGG                                                                            >  SRR4302533.3818910/1‑94 (MQ=60)
              GCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGG                                                                            <  SRR4302533.4767069/94‑1 (MQ=60)
              GCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGG                                                                            <  SRR4302533.6009032/94‑1 (MQ=60)
               CATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGT                                                                           >  SRR4302533.5291186/1‑94 (MQ=60)
                ATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGAT                                                                          <  SRR4302533.3629479/94‑1 (MQ=60)
                ATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGAT                                                                          >  SRR4302533.4313451/1‑94 (MQ=60)
                ATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGACGGAT                                                                          >  SRR4302533.140212/1‑94 (MQ=60)
                  CGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAA                                                                        <  SRR4302533.5273205/94‑1 (MQ=60)
                  CGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAA                                                                        >  SRR4302533.4162109/1‑94 (MQ=60)
                  CGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAA                                                                        >  SRR4302533.553237/1‑94 (MQ=60)
                   GTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAG                                                                       >  SRR4302533.2909653/1‑94 (MQ=60)
                   GTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAG                                                                       <  SRR4302533.3944606/94‑1 (MQ=60)
                   GTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAG                                                                       >  SRR4302533.325706/1‑94 (MQ=60)
                    TGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACTGTAGGCCGGATAAGG                                                                      >  SRR4302533.5350842/1‑94 (MQ=60)
                    TGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGG                                                                      >  SRR4302533.4206852/1‑94 (MQ=60)
                    TGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGG                                                                      >  SRR4302533.3794344/1‑94 (MQ=60)
                     GATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGC                                                                     >  SRR4302533.6069135/1‑94 (MQ=60)
                     GATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGC                                                                     <  SRR4302533.3148673/94‑1 (MQ=60)
                      ATTTCAACGATAAGTGGGACGATGAGGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCG                                                                    <  SRR4302533.1684271/94‑1 (MQ=60)
                        TTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTT                                                                  >  SRR4302533.3466368/1‑94 (MQ=60)
                        TTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTT                                                                  >  SRR4302533.3682836/1‑94 (MQ=60)
                        TTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTT                                                                  >  SRR4302533.4877852/1‑94 (MQ=60)
                          CAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAA                                                                >  SRR4302533.852762/1‑94 (MQ=60)
                          CAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAA                                                                >  SRR4302533.76263/1‑94 (MQ=60)
                          CAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAA                                                                <  SRR4302533.674865/94‑1 (MQ=60)
                             CGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGC                                                             >  SRR4302533.46535/1‑94 (MQ=60)
                             CGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGC                                                             >  SRR4302533.703264/1‑94 (MQ=60)
                              GATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCC                                                            >  SRR4302533.5569372/1‑94 (MQ=60)
                               ATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCG                                                           >  SRR4302533.2087525/1‑94 (MQ=60)
                               ATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCG                                                           <  SRR4302533.5829578/94‑1 (MQ=60)
                               ATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCG                                                           >  SRR4302533.4826456/1‑94 (MQ=60)
                                 AAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCA                                                         >  SRR4302533.4672536/1‑94 (MQ=60)
                                  AGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCAT                                                        <  SRR4302533.1987075/94‑1 (MQ=60)
                                    TGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCC                                                      <  SRR4302533.3897905/94‑1 (MQ=60)
                                     GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCG                                                     <  SRR4302533.2959219/94‑1 (MQ=60)
                                     GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCG                                                     <  SRR4302533.168420/94‑1 (MQ=60)
                                     GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCG                                                     <  SRR4302533.22770/94‑1 (MQ=60)
                                     GGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCG                                                     <  SRR4302533.45698/94‑1 (MQ=60)
                                      GGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGG                                                    <  SRR4302533.5205490/94‑1 (MQ=60)
                                      GGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGG                                                    >  SRR4302533.3757779/1‑94 (MQ=60)
                                       GACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGC                                                   >  SRR4302533.333210/1‑94 (MQ=60)
                                         CGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAA                                                 >  SRR4302533.3421430/1‑94 (MQ=60)
                                         CGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAA                                                 >  SRR4302533.3160118/1‑94 (MQ=60)
                                          GATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAT                                                >  SRR4302533.4762595/1‑94 (MQ=60)
                                          GATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAT                                                >  SRR4302533.192630/1‑94 (MQ=60)
                                          GATGACGACGACTGGACGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAT                                                <  SRR4302533.5018/94‑1 (MQ=60)
                                           ATGACGACGACTGGCCGAAAAACGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATG                                               <  SRR4302533.203264/94‑1 (MQ=60)
                                            TGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGG                                              >  SRR4302533.240426/1‑94 (MQ=60)
                                             GACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGT                                             >  SRR4302533.2374788/1‑94 (MQ=60)
                                             GACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGT                                             >  SRR4302533.121953/1‑94 (MQ=60)
                                              ACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTG                                            <  SRR4302533.117543/94‑1 (MQ=60)
                                              ACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTG                                            <  SRR4302533.4079560/94‑1 (MQ=60)
                                               CGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGA                                           <  SRR4302533.2719340/94‑1 (MQ=60)
                                               CGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGA                                           >  SRR4302533.6169098/1‑94 (MQ=60)
                                                GACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAA                                          >  SRR4302533.5762749/1‑94 (MQ=60)
                                                GACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAA                                          >  SRR4302533.2626124/1‑94 (MQ=60)
                                                GACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAA                                          >  SRR4302533.1166944/1‑94 (MQ=60)
                                                GACGACTGGAGGAAAAAGGATCAACCTAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAA                                          <  SRR4302533.5367479/94‑1 (MQ=60)
                                                 ACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAC                                         >  SRR4302533.162955/1‑94 (MQ=60)
                                                  CGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCGATGGTGAACG                                        >  SRR4302533.6271794/1‑94 (MQ=60)
                                                  CGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACG                                        <  SRR4302533.6253796/94‑1 (MQ=60)
                                                  CGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACG                                        <  SRR4302533.2131357/94‑1 (MQ=60)
                                                   GACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA                                       >  SRR4302533.4126578/1‑94 (MQ=60)
                                                     CTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATG                                     >  SRR4302533.5463621/1‑94 (MQ=60)
                                                     CTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATG                                     <  SRR4302533.1117530/94‑1 (MQ=60)
                                                      TGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGC                                    <  SRR4302533.4848161/94‑1 (MQ=60)
                                                      TGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGC                                    >  SRR4302533.3120164/1‑94 (MQ=60)
                                                       GGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCC                                   >  SRR4302533.4580734/1‑94 (MQ=60)
                                                        GCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCT                                  <  SRR4302533.741344/94‑1 (MQ=60)
                                                        GCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCT                                  <  SRR4302533.199039/94‑1 (MQ=60)
                                                        GCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCT                                  <  SRR4302533.3569262/94‑1 (MQ=60)
                                                        GCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCT                                  <  SRR4302533.5398568/94‑1 (MQ=60)
                                                         CCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTG                                 <  SRR4302533.507993/94‑1 (MQ=60)
                                                         CCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTG                                 <  SRR4302533.1050962/94‑1 (MQ=60)
                                                          CGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGCTGCCTGA                                >  SRR4302533.1750814/1‑94 (MQ=60)
                                                          CGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGA                                >  SRR4302533.5621903/1‑94 (MQ=60)
                                                          CGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGA                                >  SRR4302533.3419293/1‑94 (MQ=60)
                                                          CGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGA                                <  SRR4302533.3485384/94‑1 (MQ=60)
                                                          CGAAAAAGGATCAACCAAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGA                                <  SRR4302533.6191747/94‑1 (MQ=60)
                                                           GAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGAT                               >  SRR4302533.268929/1‑94 (MQ=60)
                                                           GAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGAT                               >  SRR4302533.1560139/1‑94 (MQ=60)
                                                           GAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGAT                               <  SRR4302533.4225274/94‑1 (MQ=60)
                                                           GAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGAT                               >  SRR4302533.3373353/1‑94 (MQ=60)
                                                           GAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGA‑AAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATG                              <  SRR4302533.4099901/94‑1 (MQ=60)
                                                            AAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATG                              >  SRR4302533.4793915/1‑94 (MQ=60)
                                                            AAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATG                              <  SRR4302533.3322200/94‑1 (MQ=60)
                                                            AAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATG                              >  SRR4302533.3730731/1‑94 (MQ=60)
                                                              AAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCG                            >  SRR4302533.4867338/1‑94 (MQ=60)
                                                               AAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGA                           >  SRR4302533.1632885/1‑94 (MQ=60)
                                                                AGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGAC                          >  SRR4302533.5912200/1‑94 (MQ=60)
                                                                 GGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACG                         <  SRR4302533.2381041/94‑1 (MQ=60)
                                                                  GATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGC                        >  SRR4302533.4452842/1‑94 (MQ=60)
                                                                   ATCACCCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCGGGCAATGGTGAACGATGCCTGATGCGACGCT                       <  SRR4302533.5195623/94‑1 (MQ=60)
                                                                    TCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTC                      >  SRR4302533.6343765/1‑94 (MQ=60)
                                                                    TCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTC                      >  SRR4302533.2475283/1‑94 (MQ=60)
                                                                     CAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCA                     <  SRR4302533.1738126/94‑1 (MQ=60)
                                                                     CAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCA                     >  SRR4302533.1487092/1‑94 (MQ=60)
                                                                        CCGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGC                  >  SRR4302533.4713149/1‑94 (MQ=60)
                                                                         CGAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCG                 >  SRR4302533.3243763/1‑94 (MQ=60)
                                                                          GAAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGT                >  SRR4302533.660469/1‑94 (MQ=60)
                                                                           AAGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTC               <  SRR4302533.965048/94‑1 (MQ=60)
                                                                            AGAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCT              <  SRR4302533.2852600/94‑1 (MQ=60)
                                                                             GAGGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTT             <  SRR4302533.4943129/94‑1 (MQ=60)
                                                                             GAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTT             >  SRR4302533.1238575/1‑94 (MQ=60)
                                                                             GAAGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTT             <  SRR4302533.1733886/94‑1 (MQ=60)
                                                                               AGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAT           >  SRR4302533.521277/1‑94 (MQ=60)
                                                                               AGTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAT           <  SRR4302533.4725875/94‑1 (MQ=60)
                                                                                GTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATC          <  SRR4302533.5003102/94‑1 (MQ=60)
                                                                                GTAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATC          >  SRR4302533.809553/1‑94 (MQ=60)
                                                                                 TAAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCA         <  SRR4302533.2140739/94‑1 (MQ=60)
                                                                                  AAGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAG        <  SRR4302533.1627004/94‑1 (MQ=60)
                                                                                   AGCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGG       <  SRR4302533.5899413/94‑1 (MQ=60)
                                                                                    GCTGAGCGCTCATCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGC      <  SRR4302533.3887379/94‑1 (MQ=60)
                                                                                    GCTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGC      <  SRR4302533.238502/94‑1 (MQ=60)
                                                                                     CTGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCC     <  SRR4302533.3274421/94‑1 (MQ=60)
                                                                                     CGGAGCGCTCACCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCC     <  SRR4302533.1460614/94‑1 (MQ=60)
                                                                                      TGAGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCA    >  SRR4302533.3121399/1‑94 (MQ=60)
                                                                                        AGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGG  <  SRR4302533.4865090/94‑1 (MQ=60)
                                                                                        AGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGG  >  SRR4302533.1009213/1‑94 (MQ=60)
                                                                                        AGCGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGG  >  SRR4302533.6090061/1‑94 (MQ=60)
                                                                                          |                                                                                           
CCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGG  >  NC_000913/2590899‑2591080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑