Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,596,026 T→C D287G (GAC→GGC)  nlpB ← lipoprotein‑34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,0260TC95.4% 826.3 / 17.3 285D287G (GAC→GGC) nlpBlipoprotein‑34
Reads supporting (aligned to +/- strand):  ref base T (4/9);  new base C (118/153);  total (123/162)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCC  >  NC_000913/2595936‑2596115
                                                                                          |                                                                                         
tGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCg                                                                                         >  2:1197323/1‑93 (MQ=255)
tGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCg                                                                                         >  2:6703659/1‑93 (MQ=255)
 gTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGc                                                                                        >  1:1251837/1‑93 (MQ=255)
 gTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGc                                                                                        <  2:7240375/93‑1 (MQ=255)
  tAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCt                                                                                       <  2:4373061/93‑1 (MQ=255)
    ggCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGt                                                                                     <  1:4773179/93‑1 (MQ=255)
    ggCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTCTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTGCTGCCAGCCGCTGt                                                                                     <  1:7393052/93‑1 (MQ=255)
     gctgctGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTc                                                                                    <  2:1439884/93‑1 (MQ=255)
      ctgctgCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   <  1:2252468/93‑1 (MQ=255)
      ctgctgCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   <  1:9470544/93‑1 (MQ=255)
      ctgctgCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   >  1:2534098/1‑93 (MQ=255)
      ctgctgCGGTTATCTAAATCGCAAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   <  2:8415444/93‑1 (MQ=255)
         ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGAc                                                                                <  2:6331338/93‑1 (MQ=255)
         ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGAc                                                                                >  1:9599985/1‑93 (MQ=255)
         ctgcGGGTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGAc                                                                                <  1:3626567/93‑1 (MQ=255)
          tgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACa                                                                               >  1:2763070/1‑93 (MQ=255)
          tgcGGTGATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACa                                                                               <  2:4233480/93‑1 (MQ=255)
            cGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGc                                                                             <  1:7822114/93‑1 (MQ=255)
            cGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGc                                                                             <  1:9159840/93‑1 (MQ=255)
             ggTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCg                                                                            >  1:1951084/1‑93 (MQ=255)
               ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCCGACAGCGGc                                                                          >  2:8523112/1‑93 (MQ=255)
               ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGc                                                                          <  1:8164146/93‑1 (MQ=255)
                 aTCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTGAGACAGCGGCtt                                                                        >  2:190001/1‑93 (MQ=255)
                 aTCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCtt                                                                        <  1:9319013/93‑1 (MQ=255)
                  tCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTta                                                                       <  2:5440672/93‑1 (MQ=255)
                   cTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTtat                                                                      <  1:3120397/93‑1 (MQ=255)
                    tAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTtata                                                                     >  1:8525700/1‑93 (MQ=255)
                     aaaTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    >  2:6448100/1‑93 (MQ=255)
                     aaaTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    >  2:1141159/1‑93 (MQ=255)
                     aaaTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    <  1:537937/93‑1 (MQ=255)
                     aaaTCGCCAACCTCCGGTTTATAGTCGCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    <  1:6078571/93‑1 (MQ=255)
                       aTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGt                                                                  >  2:5969661/1‑93 (MQ=255)
                       aTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGt                                                                  <  2:3704190/93‑1 (MQ=255)
                         cGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTa                                                                <  1:6232084/93‑1 (MQ=255)
                         cGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTa                                                                <  1:402689/93‑1 (MQ=255)
                          gCCAACCTGCAGTTTATAGTCACCGGAGGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAc                                                               <  1:4287181/93‑1 (MQ=255)
                           ccAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACg                                                              <  1:5159772/93‑1 (MQ=255)
                             aaCCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGc                                                            <  2:6075024/93‑1 (MQ=255)
                              aCCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGcc                                                           >  2:676792/1‑93 (MQ=255)
                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          <  2:2428274/93‑1 (MQ=255)
                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          <  2:8168064/93‑1 (MQ=255)
                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          >  1:8477741/1‑93 (MQ=255)
                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          >  2:303180/1‑93 (MQ=255)
                                cTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCAt                                                         >  2:8803905/1‑93 (MQ=255)
                                cTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCAt                                                         <  1:671827/93‑1 (MQ=255)
                                 tgtaGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATg                                                        <  1:1013884/90‑1 (MQ=255)
                                 tGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATg                                                        <  2:25205/93‑1 (MQ=255)
                                  gCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGt                                                       <  2:7711702/93‑1 (MQ=255)
                                      tttATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGcc                                                   >  2:7433704/1‑93 (MQ=255)
                                      tttATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGGTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTggc                                                   >  2:9610636/1‑91 (MQ=255)
                                       ttATAGTCACCGGATGTCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGccc                                                  <  1:7884013/93‑1 (MQ=255)
                                       ttATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGccc                                                  <  1:4578327/93‑1 (MQ=255)
                                        tataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCt                                                 <  1:6978509/93‑1 (MQ=255)
                                        tataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCt                                                 >  2:7377116/1‑93 (MQ=255)
                                        tataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCt                                                 <  2:5062805/93‑1 (MQ=255)
                                         ataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTg                                                >  1:8712864/1‑93 (MQ=255)
                                            gTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGa                                             >  1:4440123/1‑93 (MQ=255)
                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            <  1:1326083/93‑1 (MQ=255)
                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            <  1:8583174/93‑1 (MQ=255)
                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            <  1:3572442/93‑1 (MQ=255)
                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            >  2:4972813/1‑93 (MQ=255)
                                              cACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAc                                           <  1:3440006/93‑1 (MQ=255)
                                                ccGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACgg                                         >  2:5766158/1‑93 (MQ=255)
                                                ccGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACgg                                         >  1:8902308/1‑93 (MQ=255)
                                                   gATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTg                                      >  1:9758477/1‑93 (MQ=255)
                                                    aTGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGc                                     <  1:6444928/93‑1 (MQ=255)
                                                    aTGCCAGCCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGc                                     <  2:9127065/93‑1 (MQ=255)
                                                          ggCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGGTGTCgg                               >  2:9160521/1‑93 (MQ=255)
                                                          ggCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCgg                               <  2:5462883/93‑1 (MQ=255)
                                                           gCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGt                              >  1:3954590/1‑93 (MQ=255)
                                                            ccTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTc                             >  1:753167/1‑93 (MQ=255)
                                                              tgaaTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCAc                           <  1:5009990/90‑1 (MQ=255)
                                                              tGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGGACGGGTGCTGGCGGTCAc                           >  2:7309916/1‑93 (MQ=255)
                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          >  1:2106466/1‑93 (MQ=255)
                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          <  1:9971569/93‑1 (MQ=255)
                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          >  2:7832649/1‑93 (MQ=255)
                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          >  2:4624199/1‑93 (MQ=255)
                                                                gATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACtt                         <  1:1833605/93‑1 (MQ=255)
                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        >  2:3598630/1‑93 (MQ=255)
                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        <  2:4019015/93‑1 (MQ=255)
                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        >  2:3193654/1‑93 (MQ=255)
                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        <  2:3421653/93‑1 (MQ=255)
                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGCGCTGTCGGTCACttt                        >  2:5013967/1‑93 (MQ=255)
                                                                   cgctcgcGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCa                      <  1:5563186/93‑1 (MQ=255)
                                                                   cgctcgcGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCa                      <  1:1691708/93‑1 (MQ=255)
                                                                       cgcgCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGcc                  <  1:617504/93‑1 (MQ=255)
                                                                         cgcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGTTGTCGGTCACTTTCATGCCCa                <  2:5210078/93‑1 (MQ=255)
                                                                         cgcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCa                <  2:2731524/93‑1 (MQ=255)
                                                                         cgcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCa                <  1:1596786/93‑1 (MQ=255)
                                                                          gcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAc               >  1:8823617/1‑93 (MQ=255)
                                                                          gcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACAGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAc               >  2:2820566/1‑93 (MQ=255)
                                                                            ccAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACtt             <  1:6483144/93‑1 (MQ=255)
                                                                             cAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACttt            >  1:5746923/1‑93 (MQ=255)
                                                                                 tCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTcc        <  2:9577633/93‑1 (MQ=255)
                                                                                  ccTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCa       <  2:3689474/93‑1 (MQ=255)
                                                                                  ccTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCa       <  2:9366343/93‑1 (MQ=255)
                                                                                  ccTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCa       <  2:9551308/93‑1 (MQ=255)
                                                                                     gCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAgcg    >  2:2296836/1‑93 (MQ=255)
                                                                                      ccAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAgcgc   >  2:7054829/1‑93 (MQ=255)
                                                                                       cAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGcgcc  >  2:9385635/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCC  >  NC_000913/2595936‑2596115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCT  >  NC_000913/2595933‑2596118
                                                                                             |                                                                                            
AACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGC                                                                                              >  SRR4302540.4894799/1‑94 (MQ=60)
 ACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCC                                                                                             >  SRR4302540.4837362/1‑94 (MQ=60)
  CTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCG                                                                                            >  SRR4302540.4889194/1‑94 (MQ=60)
  CTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCG                                                                                            >  SRR4302540.6503666/1‑94 (MQ=60)
  CTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCG                                                                                            <  SRR4302540.9316194/94‑1 (MQ=60)
   TGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGC                                                                                           >  SRR4302540.8963568/1‑94 (MQ=60)
   TGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGC                                                                                           >  SRR4302540.1264618/1‑94 (MQ=60)
    GTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCT                                                                                          <  SRR4302540.2219354/94‑1 (MQ=60)
    GTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCT                                                                                          <  SRR4302540.2077590/94‑1 (MQ=60)
    GTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCT                                                                                          <  SRR4302540.6258243/94‑1 (MQ=60)
       GGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTC                                                                                       <  SRR4302540.4824170/94‑1 (MQ=60)
       GGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCGCGCGCCCAGTTCCTGCCAGCCGCTGTC                                                                                       <  SRR4302540.4824169/94‑1 (MQ=60)
       GGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTCTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTGCTGCCAGCCGCTGTC                                                                                       <  SRR4302540.7475432/94‑1 (MQ=60)
        GCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCA                                                                                      >  SRR4302540.2561430/1‑94 (MQ=60)
        GCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCA                                                                                      <  SRR4302540.9696043/94‑1 (MQ=60)
         CTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAG                                                                                     <  SRR4302540.9582881/94‑1 (MQ=60)
         CTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAG                                                                                     <  SRR4302540.2276644/94‑1 (MQ=60)
           GCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGAC                                                                                   >  SRR4302540.9713236/1‑94 (MQ=60)
            CTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACA                                                                                  <  SRR4302540.3603508/94‑1 (MQ=60)
            CTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACA                                                                                  >  SRR4302540.2792920/1‑94 (MQ=60)
            CTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACA                                                                                  <  SRR4302540.6534589/94‑1 (MQ=60)
            CTGCGGGTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACA                                                                                  <  SRR4302540.3666255/94‑1 (MQ=60)
               CGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCG                                                                               <  SRR4302540.7913525/94‑1 (MQ=60)
               CGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCG                                                                               >  SRR4302540.9492464/1‑94 (MQ=60)
               CGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCG                                                                               <  SRR4302540.9269781/94‑1 (MQ=60)
               CGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCG                                                                               <  SRR4302540.5398419/94‑1 (MQ=60)
               CGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCG                                                                               >  SRR4302540.1972311/1‑94 (MQ=60)
                  TTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCT                                                                            <  SRR4302540.4327846/94‑1 (MQ=60)
                  TTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCT                                                                            <  SRR4302540.8258952/94‑1 (MQ=60)
                    ATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTA                                                                          >  SRR4302540.4516693/1‑94 (MQ=60)
                    ATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTA                                                                          <  SRR4302540.9430212/94‑1 (MQ=60)
                    ATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTA                                                                          >  SRR4302540.6710506/1‑94 (MQ=60)
                     TCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTAT                                                                         <  SRR4302540.5397547/94‑1 (MQ=60)
                     TCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTAT                                                                         >  SRR4302540.9076613/1‑94 (MQ=60)
                      CTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATA                                                                        <  SRR4302540.5075647/94‑1 (MQ=60)
                      CTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATA                                                                        <  SRR4302540.3154501/94‑1 (MQ=60)
                      CTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATA                                                                        <  SRR4302540.3224764/94‑1 (MQ=60)
                      CTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATA                                                                        <  SRR4302540.1689217/94‑1 (MQ=60)
                      CTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATA                                                                        >  SRR4302540.8624765/1‑94 (MQ=60)
                        AAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAG                                                                      <  SRR4302540.543758/94‑1 (MQ=60)
                        AAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAG                                                                      <  SRR4302540.6146169/94‑1 (MQ=60)
                        AAATCGCCAACCTCCGGTTTATAGTCGCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAG                                                                      <  SRR4302540.6146164/94‑1 (MQ=60)
                          ATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTT                                                                    >  SRR4302540.3863144/1‑94 (MQ=60)
                          ATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTT                                                                    >  SRR4302540.4002667/1‑94 (MQ=60)
                          ATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTT                                                                    >  SRR4302540.3751474/1‑94 (MQ=60)
                            CGCCACCCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAC                                                                  <  SRR4302540.9345166/94‑1 (MQ=60)
                            CGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAC                                                                  >  SRR4302540.7971090/1‑94 (MQ=60)
                            CGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAC                                                                  <  SRR4302540.6057351/94‑1 (MQ=60)
                            CGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAC                                                                  <  SRR4302540.406888/94‑1 (MQ=60)
                            CGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAC                                                                  <  SRR4302540.2043576/94‑1 (MQ=60)
                            CGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAC                                                                  <  SRR4302540.6301398/94‑1 (MQ=60)
                             GCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACG                                                                 >  SRR4302540.7655442/1‑94 (MQ=60)
                             GCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCAGCTGGCAGCCCGCGGCTTATAAGTTACG                                                                 >  SRR4302540.7655443/1‑94 (MQ=60)
                             GCCAACCTGCAGTTTATAGTCACCGGAGGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACG                                                                 <  SRR4302540.4333813/94‑1 (MQ=60)
                              CCAACCTGCAGTTTATAGTCACCGGATGCCAGTCCTGTATCGCTCGCGCCCCGTTCCTGCCAGCCGCTGTCAGACAGCGGATTATAAGTTACGG                                                                >  SRR4302540.3569976/1‑94 (MQ=60)
                              CCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGG                                                                >  SRR4302540.9110270/1‑94 (MQ=60)
                              CCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGG                                                                <  SRR4302540.5215854/94‑1 (MQ=60)
                               CAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGC                                                               <  SRR4302540.578132/94‑1 (MQ=60)
                               CAACCGGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGC                                                               <  SRR4302540.578131/94‑1 (MQ=60)
                                AACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCC                                                              >  SRR4302540.621848/1‑94 (MQ=60)
                                AACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCC                                                              <  SRR4302540.2290524/94‑1 (MQ=60)
                                AACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCACCCGCTGTCAGCAAGCGGCTTACAAGTTCCCGCC                                                              >  SRR4302540.8093030/1‑94 (MQ=60)
                                 ACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCA                                                             >  SRR4302540.8576208/1‑94 (MQ=60)
                                 ACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCA                                                             >  SRR4302540.2725314/1‑94 (MQ=60)
                                  CCTGGAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCAT                                                            <  SRR4302540.3337030/94‑1 (MQ=60)
                                  CCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCAT                                                            >  SRR4302540.1383949/1‑94 (MQ=60)
                                   CTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATG                                                           <  SRR4302540.679026/94‑1 (MQ=60)
                                   CTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATG                                                           <  SRR4302540.8283750/94‑1 (MQ=60)
                                    TGTAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGT                                                          <  SRR4302540.1024160/94‑1 (MQ=60)
                                    TGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGT                                                          >  SRR4302540.251125/1‑94 (MQ=60)
                                     GCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTT                                                         <  SRR4302540.1719252/94‑1 (MQ=60)
                                      CAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTG                                                        <  SRR4302540.6095973/94‑1 (MQ=60)
                                        GTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCC                                                      >  SRR4302540.4432219/1‑94 (MQ=60)
                                        GTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCC                                                      <  SRR4302540.4581024/94‑1 (MQ=60)
                                         TTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCC                                                     <  SRR4302540.5130524/94‑1 (MQ=60)
                                          TTATAGTCACCGGATGTCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCT                                                    <  SRR4302540.7976064/94‑1 (MQ=60)
                                          TTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCT                                                    <  SRR4302540.6269567/94‑1 (MQ=60)
                                          TTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCT                                                    <  SRR4302540.4627699/94‑1 (MQ=60)
                                           TATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTG                                                   <  SRR4302540.7056037/94‑1 (MQ=60)
                                           TATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCCTGTTGACCTG                                                   >  SRR4302540.7532613/1‑94 (MQ=60)
                                           TATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTG                                                   <  SRR4302540.10042449/94‑1 (MQ=60)
                                           TATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTG                                                   >  SRR4302540.8814013/1‑94 (MQ=60)
                                           TATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACCGCCATGTTGCCCTG                                                   >  SRR4302540.8813752/1‑94 (MQ=60)
                                            ATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGC                                                  <  SRR4302540.9487359/94‑1 (MQ=60)
                                              AGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGA                                                >  SRR4302540.4488238/1‑94 (MQ=60)
                                                TCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAC                                              <  SRR4302540.1339917/94‑1 (MQ=60)
                                                TCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAC                                              >  SRR4302540.4737803/1‑94 (MQ=60)
                                                TCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAC                                              >  SRR4302540.8685958/1‑94 (MQ=60)
                                                TCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAC                                              <  SRR4302540.8682954/94‑1 (MQ=60)
                                                TCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAC                                              <  SRR4302540.3611465/94‑1 (MQ=60)
                                                GCACCGGATCCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAC                                              <  SRR4302540.6776815/94‑1 (MQ=60)
                                                 CACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACG                                             >  SRR4302540.9630393/1‑94 (MQ=60)
                                                 CACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACG                                             <  SRR4302540.3477403/94‑1 (MQ=60)
                                                  ACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGG                                            >  SRR4302540.9005367/1‑94 (MQ=60)
                                                     GGATGCCAGGCCTGTATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTG                                         <  SRR4302540.10027316/94‑1 (MQ=60)
                                                     GGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTG                                         >  SRR4302540.6801418/1‑94 (MQ=60)
                                                     GGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTG                                         >  SRR4302540.9872947/1‑94 (MQ=60)
                                                      GATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGC                                        <  SRR4302540.487404/94‑1 (MQ=60)
                                                       ATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCT                                       <  SRR4302540.6516675/94‑1 (MQ=60)
                                                       ATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCT                                       <  SRR4302540.859783/94‑1 (MQ=60)
                                                       ATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCT                                       <  SRR4302540.2945408/94‑1 (MQ=60)
                                                        TGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTG                                      >  SRR4302540.1276299/1‑94 (MQ=60)
                                                        TGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTG                                      <  SRR4302540.7231473/94‑1 (MQ=60)
                                                          CCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTC                                    <  SRR4302540.801858/94‑1 (MQ=60)
                                                           CAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCG                                   <  SRR4302540.2227783/94‑1 (MQ=60)
                                                            AGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGG                                  <  SRR4302540.1909894/94‑1 (MQ=60)
                                                            AGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGGCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGG                                  >  SRR4302540.86475/1‑94 (MQ=60)
                                                             GGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGT                                 >  SRR4302540.2818641/1‑94 (MQ=60)
                                                             GGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGT                                 >  SRR4302540.3997268/1‑94 (MQ=60)
                                                              GCCTGGATCGCTCGCGCCCAGTTCCTGCCAGGCGCTGTCAGACAGCGGCCTATAAGGTACGGCCATGTTGCCCTGCGGACGGGTGCTGTTGGTC                                >  SRR4302540.8910371/1‑94 (MQ=60)
                                                              GCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTC                                >  SRR4302540.761192/1‑94 (MQ=60)
                                                                CTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCAC                              >  SRR4302540.6380413/1‑94 (MQ=60)
                                                                 TGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACT                             >  SRR4302540.2129073/1‑94 (MQ=60)
                                                                 TGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACT                             >  SRR4302540.2678653/1‑94 (MQ=60)
                                                                 TGAATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACT                             <  SRR4302540.5064241/94‑1 (MQ=60)
                                                                  GGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTT                            >  SRR4302540.4014103/1‑94 (MQ=60)
                                                                  GGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTT                            <  SRR4302540.10087883/94‑1 (MQ=60)
                                                                   GATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTT                           <  SRR4302540.1853671/94‑1 (MQ=60)
                                                                   GATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTT                           <  SRR4302540.1853672/94‑1 (MQ=60)
                                                                    ATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCAAGTTGCTCTGCTACCGGGTGCGGTCGGGCACTTTC                          >  SRR4302540.3665072/1‑94 (MQ=60)
                                                                      CGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCAT                        <  SRR4302540.1710053/94‑1 (MQ=60)
                                                                      CGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCAT                        <  SRR4302540.1117521/94‑1 (MQ=60)
                                                                      CGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCAT                        <  SRR4302540.5624446/94‑1 (MQ=60)
                                                                      CGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCAT                        <  SRR4302540.7371525/94‑1 (MQ=60)
                                                                      CGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCAT                        <  SRR4302540.131193/94‑1 (MQ=60)
                                                                      CGCTCGCGCCCAGTGCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCAT                        <  SRR4302540.1701594/94‑1 (MQ=60)
                                                                       GCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATG                       >  SRR4302540.796281/1‑94 (MQ=60)
                                                                         TCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCC                     <  SRR4302540.4415128/94‑1 (MQ=60)
                                                                         TCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCC                     <  SRR4302540.8853920/94‑1 (MQ=60)
                                                                          CGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCC                    <  SRR4302540.624088/94‑1 (MQ=60)
                                                                            CGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAC                  <  SRR4302540.9381364/94‑1 (MQ=60)
                                                                            CGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAC                  >  SRR4302540.8925652/1‑94 (MQ=60)
                                                                            CGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAC                  >  SRR4302540.8925651/1‑94 (MQ=60)
                                                                            CGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAC                  <  SRR4302540.1614237/94‑1 (MQ=60)
                                                                             GCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTTTAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCCTGTCCACT                 >  SRR4302540.1883323/1‑94 (MQ=60)
                                                                             GCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGCACGGGGGCTGTCGGTCGCTTTTATGCACACT                 >  SRR4302540.1883324/1‑94 (MQ=60)
                                                                             GCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACT                 <  SRR4302540.1443080/94‑1 (MQ=60)
                                                                               CCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTATGGCCATGTTGACCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTT               >  SRR4302540.6524023/1‑94 (MQ=60)
                                                                               CCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTT               >  SRR4302540.5810311/1‑94 (MQ=60)
                                                                               CCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTT               <  SRR4302540.6555253/94‑1 (MQ=60)
                                                                               CCAGCTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTT               >  SRR4302540.5456948/1‑94 (MQ=60)
                                                                                CACTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTT              <  SRR4302540.9726029/94‑1 (MQ=60)
                                                                                    TCCTGCCAGTACCTGTCAGACAGCGGCTTATAAGTTTCGGCCATGGTGCCCTGCGAACGGGTGCTGTGGGTCACTTTCATGCCCCCTTTTTCCA          <  SRR4302540.8433600/94‑1 (MQ=60)
                                                                                    TCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCA          <  SRR4302540.5573221/94‑1 (MQ=60)
                                                                                    TCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCA          <  SRR4302540.6977224/94‑1 (MQ=60)
                                                                                     CCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAG         <  SRR4302540.3639200/94‑1 (MQ=60)
                                                                                       TGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCG       <  SRR4302540.8581643/94‑1 (MQ=60)
                                                                                       TGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCG       <  SRR4302540.9750866/94‑1 (MQ=60)
                                                                                         CCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCC     <  SRR4302540.8634905/94‑1 (MQ=60)
                                                                                          CAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCG    >  SRR4302540.6507858/1‑94 (MQ=60)
                                                                                            GCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGCACGGGTGCTGTCGGGCACTTTCATGCCCACTTTTTCCAGCGCCGCT  >  SRR4302540.7266893/1‑94 (MQ=60)
                                                                                            GCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCT  <  SRR4302540.3166342/94‑1 (MQ=60)
                                                                                            GCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCT  <  SRR4302540.8204391/94‑1 (MQ=60)
                                                                                            GCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCT  >  SRR4302540.7266894/1‑94 (MQ=60)
                                                                                             |                                                                                            
AACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCT  >  NC_000913/2595933‑2596118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑