Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,596,487	T→C	Q133Q (CAA→CAG)	nlpB ←	lipoprotein‑34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,596,487	0	T	C	99.6%	822.1 / ‑5.4	255	Q133Q (CAA→CAG)	nlpB	lipoprotein‑34
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (108/146); total (109/146)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 258 total aligned reads displayed.

GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGC  >  NC_000913/2596397‑2596576

 aTTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTgg                                                                                        >  1:5661284/1‑93 (MQ=255)

 aTTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTgg                                                                                        <  1:5895775/93‑1 (MQ=255)

 aTTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTgg                                                                                        >  1:3411183/1‑93 (MQ=255)

  tttGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTggg                                                                                       >  1:2299538/1‑93 (MQ=255)

   ttGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGt                                                                                      <  1:9853740/93‑1 (MQ=255)

   ttGAGAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGt                                                                                      <  2:3093868/93‑1 (MQ=255)

     gATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGa                                                                                    <  1:5230344/93‑1 (MQ=255)

      aTAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAt                                                                                   >  1:9427320/1‑93 (MQ=255)

      aTAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAt                                                                                   <  2:9628204/93‑1 (MQ=255)

       tAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATg                                                                                  <  1:5958942/93‑1 (MQ=255)

       tAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATg                                                                                  >  1:1528441/1‑93 (MQ=255)

        aaCGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATgg                                                                                 >  2:7883267/1‑93 (MQ=255)

           gACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGgtgt                                                                              <  2:6176629/93‑1 (MQ=255)

            aCCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTa                                                                             >  1:6277492/1‑93 (MQ=255)

            aCCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTa                                                                             >  1:1040898/1‑93 (MQ=255)

            aCCACGATACTGCTCGTCTTCGTCCAGACGGTTCCAATGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTa                                                                             <  1:4362487/93‑1 (MQ=255)

                 gATACTGCTCGTCTTCGTCCAGACGGTTCCATGGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGtttt                                                                        <  1:820191/93‑1 (MQ=255)

                      tGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGcc                                                                   <  1:3328855/93‑1 (MQ=255)

                      tGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGcc                                                                   <  1:3610825/93‑1 (MQ=255)

                         tCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgc                                                                >  2:4767861/1‑93 (MQ=255)

                         tCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgc                                                                >  1:7613187/1‑93 (MQ=255)

                         tCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgc                                                                <  2:1244777/93‑1 (MQ=255)

                          cGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgca                                                               >  1:1526372/1‑93 (MQ=255)

                          cGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgca                                                               >  1:9203980/1‑93 (MQ=255)

                           gTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgcag                                                              <  2:9219094/93‑1 (MQ=255)

                             cTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgcagca                                                            <  2:6903853/93‑1 (MQ=255)

                              ctCGTCCAGACGGTTCCGTTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCAc                                                           <  2:4580601/92‑1 (MQ=255)

                                cGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGc                                                         <  2:6949538/93‑1 (MQ=255)

                                cGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTGTTTCGCCTGCAGCACGc                                                         <  1:4053241/93‑1 (MQ=255)

                                 gTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCt                                                        <  2:7210023/93‑1 (MQ=255)

                                  tCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTa                                                       >  2:2708947/1‑93 (MQ=255)

                                    cAGACGGTTCCATTTTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAac                                                     <  2:5620202/93‑1 (MQ=255)

                                    cAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAac                                                     <  1:5544218/93‑1 (MQ=255)

                                     aGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAacc                                                    >  2:4977336/1‑93 (MQ=255)

                                       actgTTCCCTTTTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAaccac                                                  <  1:3189941/90‑1 (MQ=255)

                                       aCGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAaccac                                                  <  2:5173980/93‑1 (MQ=255)

                                        cGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAaccacc                                                 <  1:5464377/93‑1 (MQ=255)

                                        cGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAaccacc                                                 <  2:7050199/93‑1 (MQ=255)

                                         ggTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCt                                                <  2:4510396/93‑1 (MQ=255)

                                          gTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTg                                               >  1:1134573/1‑93 (MQ=255)

                                            tCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCg                                             <  1:669332/93‑1 (MQ=255)

                                              cATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGc                                           >  2:3963130/1‑93 (MQ=255)

                                               aTTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGcc                                          >  2:9038626/1‑93 (MQ=255)

                                               aTTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGcc                                          >  1:9790865/1‑93 (MQ=255)

                                                ttGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCca                                         <  2:5641485/93‑1 (MQ=255)

                                                ttGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCca                                         >  2:8518141/1‑93 (MQ=255)

                                                  gTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCcaca                                       <  1:2216576/93‑1 (MQ=255)

                                                     cccAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACagag                                    <  1:9983770/93‑1 (MQ=255)

                                                     cccAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACagag                                    <  1:2838375/93‑1 (MQ=255)

                                                     cccAATCGGTGGGCAGTGTCTGACCAGCATCATCACGCTGGATGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACagag                                    <  2:4668552/93‑1 (MQ=255)

                                                      ccAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGt                                   <  2:2802504/93‑1 (MQ=255)

                                                       cAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTa                                  <  1:738252/93‑1 (MQ=255)

                                                       cAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTa                                  <  1:181140/93‑1 (MQ=255)

                                                       cAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTa                                  <  1:2173532/93‑1 (MQ=255)

                                                        aaTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAt                                 <  1:9634092/93‑1 (MQ=255)

                                                        aaTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAt                                 >  1:4661475/1‑93 (MQ=255)

                                                        aaTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAt                                 <  1:7787019/93‑1 (MQ=255)

                                                         aTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGGGATGGGGTAGTTTTTCGCCTGCACTACGCTAACCACCTCCGCACACACAGTAtc                                >  2:75794/1‑92 (MQ=255)

                                                          tCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTg                               <  2:9924997/93‑1 (MQ=255)

                                                           cGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGc                              <  2:2527980/93‑1 (MQ=255)

                                                            ggtggtCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGcc                             >  1:7788210/1‑93 (MQ=255)

                                                             gtggtCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCa                            >  2:1827987/1‑93 (MQ=255)

                                                              tgggCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCAc                           <  2:4314419/93‑1 (MQ=255)

                                                                 tCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGAc                        >  1:9017168/1‑93 (MQ=255)

                                                                 gcAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGAc                        <  2:2219080/92‑1 (MQ=255)

                                                                   aGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCa                      <  2:8072911/93‑1 (MQ=255)

                                                                    gtgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAt                     >  1:8092491/1‑93 (MQ=255)

                                                                    gtgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAt                     <  1:2711609/93‑1 (MQ=255)

                                                                     tgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtt                    >  1:4093473/1‑93 (MQ=255)

                                                                     tgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtt                    >  2:877167/1‑93 (MQ=255)

                                                                     tgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtt                    >  1:6299255/1‑93 (MQ=255)

                                                                     tgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtt                    <  1:7480134/93‑1 (MQ=255)

                                                                       tCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtttt                  >  1:1970240/1‑93 (MQ=255)

                                                                       tCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtttt                  >  2:2404602/1‑93 (MQ=255)

                                                                        cTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTc                 <  2:957387/93‑1 (MQ=255)

                                                                          gatcAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGACCACAGAGTATTGCCACGACCATTTTcca               <  1:6757506/90‑1 (MQ=255)

                                                                          gACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCTCCTGCGGCCACAGAGTATTGCCACGACCATTTTcca               <  2:8446336/93‑1 (MQ=255)

                                                                          gACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTcca               <  1:5783748/93‑1 (MQ=255)

                                                                           aCCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTccac              <  1:8342833/93‑1 (MQ=255)

                                                                             cAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTccacca            <  2:4096617/93‑1 (MQ=255)

                                                                              aGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAg           >  2:6868961/1‑93 (MQ=255)

                                                                               gCATCGTCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGc          <  1:7784692/93‑1 (MQ=255)

                                                                                catcatCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCa         <  2:5432568/93‑1 (MQ=255)

                                                                                catcatCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCa         <  2:6509815/93‑1 (MQ=255)

                                                                                 atcatcACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCaa        <  2:8018534/93‑1 (MQ=255)

                                                                                 atcatcACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCaa        <  2:1363012/93‑1 (MQ=255)

                                                                                 atcaccACGCTGGGTGATGGTGTAGTTTTCCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCaa        <  1:8843728/93‑1 (MQ=255)

                                                                                  tcatcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCGCAGAGTATTGCCACGACCATTTTCCACCAGCAAt       >  2:6745581/1‑93 (MQ=255)

                                                                                  tcatcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAt       >  1:3821198/1‑93 (MQ=255)

                                                                                  tcatcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAt       >  1:2414712/1‑93 (MQ=255)

                                                                                   catcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATg      <  2:7431662/93‑1 (MQ=255)

                                                                                   catcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATg      <  2:1142691/93‑1 (MQ=255)

                                                                                    atcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCGCCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGa     <  1:5928819/93‑1 (MQ=255)

                                                                                    atcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGa     >  2:3220132/1‑93 (MQ=255)

                                                                                    atcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGa     >  1:8994830/1‑93 (MQ=255)

                                                                                    atcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGa     >  1:9441031/1‑93 (MQ=255)

                                                                                     tcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGaa    <  2:5936185/93‑1 (MQ=255)

                                                                                     tcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGaa    >  1:6554757/1‑93 (MQ=255)

                                                                                      caCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAg   >  1:1751467/1‑93 (MQ=255)

                                                                                       aCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGc  <  2:3424839/93‑1 (MQ=255)

GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGC  >  NC_000913/2596397‑2596576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGTA  >  NC_000913/2596394‑2596580

AGAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTCCCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGC                                                                                               <  SRR4302540.6535956/94‑1 (MQ=60)

AGAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGC                                                                                               >  SRR4302540.1946408/1‑94 (MQ=60)

 GAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCT                                                                                              >  SRR4302540.7734262/1‑94 (MQ=60)

   GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGG                                                                                            <  SRR4302540.8259717/94‑1 (MQ=60)

   GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGG                                                                                            >  SRR4302540.3448335/1‑94 (MQ=60)

   GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGG                                                                                            >  SRR4302540.5723624/1‑94 (MQ=60)

    ATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGG                                                                                           >  SRR4302540.2324273/1‑94 (MQ=60)

    ATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGG                                                                                           <  SRR4302540.5960887/94‑1 (MQ=60)

     TTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGT                                                                                          <  SRR4302540.3489702/94‑1 (MQ=60)

      TTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTG                                                                                         <  SRR4302540.9968899/94‑1 (MQ=60)

       TGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGA                                                                                        >  SRR4302540.9924156/1‑94 (MQ=60)

        GATATCGACCACGATACGGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCATTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAT                                                                                       <  SRR4302540.5474687/94‑1 (MQ=60)

        GATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAT                                                                                       <  SRR4302540.5287383/94‑1 (MQ=60)

        GATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAT                                                                                       >  SRR4302540.9539328/1‑94 (MQ=60)

         ATAACGGCCACGATACCGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATG                                                                                      <  SRR4302540.3243943/94‑1 (MQ=60)

         ATAACGCCCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATG                                                                                      <  SRR4302540.5576604/94‑1 (MQ=60)

         ATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATG                                                                                      >  SRR4302540.1545368/1‑94 (MQ=60)

          TAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGG                                                                                     <  SRR4302540.6024850/94‑1 (MQ=60)

            ACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTG                                                                                   >  SRR4302540.9147773/1‑94 (MQ=60)

            ACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTG                                                                                   <  SRR4302540.9102036/94‑1 (MQ=60)

             CGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGT                                                                                  <  SRR4302540.1934591/94‑1 (MQ=60)

              GACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTA                                                                                 >  SRR4302540.6347330/1‑94 (MQ=60)

              GACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTA                                                                                 >  SRR4302540.1051481/1‑94 (MQ=60)

               ACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCAATGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAG                                                                                <  SRR4302540.4409915/94‑1 (MQ=60)

                CCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGT                                                                               <  SRR4302540.1137806/94‑1 (MQ=60)

                CCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGT                                                                               <  SRR4302540.5671918/94‑1 (MQ=60)

                ACACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTTCCCAATCGGTGGGCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGT                                                                               <  SRR4302540.3544395/94‑1 (MQ=60)

                  ACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTT                                                                             >  SRR4302540.2272673/1‑94 (MQ=60)

                    GATACTGCTCGTCTTCGTCCAGACGGTTCCATGGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTT                                                                           <  SRR4302540.828907/94‑1 (MQ=60)

                     ATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTC                                                                          <  SRR4302540.8259759/94‑1 (MQ=60)

                     ATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTC                                                                          <  SRR4302540.5982742/94‑1 (MQ=60)

                      TGCTGCTCGTCTGCGTCCAGACGGTTCCATTGTACCCGATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCG                                                                         <  SRR4302540.7352468/94‑1 (MQ=60)

                      TACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCG                                                                         <  SRR4302540.3407293/94‑1 (MQ=60)

                         TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                      <  SRR4302540.3365396/94‑1 (MQ=60)

                         TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                      <  SRR4302540.3650292/94‑1 (MQ=60)

                         TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                      <  SRR4302540.7665139/94‑1 (MQ=60)

                         TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                      <  SRR4302540.6354214/94‑1 (MQ=60)

                         CGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                      <  SRR4302540.2232673/94‑1 (MQ=60)

                         CGCTCGTCTGCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                      <  SRR4302540.3650293/94‑1 (MQ=60)

                          GCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGACTG                                                                     >  SRR4302540.5715267/1‑94 (MQ=60)

                           CTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGC                                                                    >  SRR4302540.7698767/1‑94 (MQ=60)

                           CTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGC                                                                    <  SRR4302540.7787877/94‑1 (MQ=60)

                            TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                   >  SRR4302540.278992/1‑94 (MQ=60)

                            TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                   >  SRR4302540.9314416/1‑94 (MQ=60)

                            TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                   >  SRR4302540.6021614/1‑94 (MQ=60)

                            TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                   >  SRR4302540.1543280/1‑94 (MQ=60)

                            TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                   <  SRR4302540.5881859/94‑1 (MQ=60)

                              GTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGC                                                                 <  SRR4302540.5966520/94‑1 (MQ=60)

                                CTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCAC                                                               <  SRR4302540.5360743/94‑1 (MQ=60)

                                   CGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCT                                                            <  SRR4302540.4096944/94‑1 (MQ=60)

                                   CGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTGTTTCGCCTGCAGCACGCT                                                            <  SRR4302540.4096943/94‑1 (MQ=60)

                                    GTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTA                                                           >  SRR4302540.3280566/1‑94 (MQ=60)

                                     TCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAA                                                          >  SRR4302540.9927751/1‑94 (MQ=60)

                                     TCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAA                                                          <  SRR4302540.9545914/94‑1 (MQ=60)

                                     TCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAA                                                          >  SRR4302540.3008962/1‑94 (MQ=60)

                                       CAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACC                                                        <  SRR4302540.5605290/94‑1 (MQ=60)

                                         GACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCAC                                                      >  SRR4302540.9636387/1‑94 (MQ=60)

                                          ACTGTTCCCTTTTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACC                                                     <  SRR4302540.3224594/94‑1 (MQ=60)

                                           CGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCT                                                    <  SRR4302540.5524520/94‑1 (MQ=60)

                                           CGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCT                                                    <  SRR4302540.1543921/94‑1 (MQ=60)

                                            GGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTG                                                   <  SRR4302540.5740684/94‑1 (MQ=60)

                                            GGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTG                                                   >  SRR4302540.1146399/1‑94 (MQ=60)

                                            GGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGGTTTTCGCCTGCAGCACGCTAACCACCTG                                                   >  SRR4302540.746942/1‑94 (MQ=60)

                                             GTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGC                                                  <  SRR4302540.4530065/94‑1 (MQ=60)

                                              TTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCG                                                 <  SRR4302540.7446633/94‑1 (MQ=60)

                                              TTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCG                                                 >  SRR4302540.2640460/1‑94 (MQ=60)

                                              TTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCG                                                 >  SRR4302540.6567147/1‑94 (MQ=60)

                                               TCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGG                                                <  SRR4302540.676510/94‑1 (MQ=60)

                                                CCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGC                                               >  SRR4302540.6687632/1‑94 (MQ=60)

                                                 CATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCC                                              >  SRR4302540.9905561/1‑94 (MQ=60)

                                                 CATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCC                                              >  SRR4302540.4710100/1‑94 (MQ=60)

                                                   TTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCAC                                            >  SRR4302540.1572354/1‑94 (MQ=60)

                                                   TTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCAC                                            <  SRR4302540.1315388/94‑1 (MQ=60)

                                                     GTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAG                                          <  SRR4302540.2240494/94‑1 (MQ=60)

                                                       ACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAG                                        <  SRR4302540.7869180/94‑1 (MQ=60)

                                                        CCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGT                                       >  SRR4302540.6675325/1‑94 (MQ=60)

                                                        CCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGT                                       <  SRR4302540.10100171/94‑1 (MQ=60)

                                                        CCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGT                                       <  SRR4302540.2869077/94‑1 (MQ=60)

                                                         CCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTA                                      <  SRR4302540.8660185/94‑1 (MQ=60)

                                                          CAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAT                                     <  SRR4302540.746133/94‑1 (MQ=60)

                                                          CAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAT                                     >  SRR4302540.4711377/1‑94 (MQ=60)

                                                          CAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAT                                     <  SRR4302540.2196972/94‑1 (MQ=60)

                                                          CAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAT                                     <  SRR4302540.182944/94‑1 (MQ=60)

                                                           AATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATT                                    >  SRR4302540.1405977/1‑94 (MQ=60)

                                                           AATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATT                                    <  SRR4302540.7878041/94‑1 (MQ=60)

                                                           AATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATT                                    <  SRR4302540.7878043/94‑1 (MQ=60)

                                                           AATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATT                                    <  SRR4302540.9747601/94‑1 (MQ=60)

                                                            ATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTG                                   >  SRR4302540.2290334/1‑94 (MQ=60)

                                                             TCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGC                                  <  SRR4302540.4076507/94‑1 (MQ=60)

                                                              CGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCC                                 >  SRR4302540.7879247/1‑94 (MQ=60)

                                                              CGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCC                                 >  SRR4302540.7879246/1‑94 (MQ=60)

                                                               GGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCA                                <  SRR4302540.5758428/94‑1 (MQ=60)

                                                                 TGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACG                              >  SRR4302540.1647614/1‑94 (MQ=60)

                                                                  GGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGA                             >  SRR4302540.1828526/1‑94 (MQ=60)

                                                                   GTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGAC                            >  SRR4302540.8313113/1‑94 (MQ=60)

                                                                   GTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGAC                            >  SRR4302540.9125366/1‑94 (MQ=60)

                                                                   GTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGAC                            >  SRR4302540.9125365/1‑94 (MQ=60)

                                                                      AGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAT                         >  SRR4302540.8186427/1‑94 (MQ=60)

                                                                      AGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAT                         >  SRR4302540.9136863/1‑94 (MQ=60)

                                                                      AGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAT                         >  SRR4302540.2083336/1‑94 (MQ=60)

                                                                       GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                        >  SRR4302540.41176/1‑94 (MQ=60)

                                                                       GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                        >  SRR4302540.4137636/1‑94 (MQ=60)

                                                                       GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                        >  SRR4302540.6369400/1‑94 (MQ=60)

                                                                       GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                        <  SRR4302540.2740848/94‑1 (MQ=60)

                                                                        TGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTT                       <  SRR4302540.7563615/94‑1 (MQ=60)

                                                                         GTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTT                      <  SRR4302540.2918217/94‑1 (MQ=60)

                                                                         GTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTT                      >  SRR4302540.1991630/1‑94 (MQ=60)

                                                                         GTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTT                      <  SRR4302540.2114883/94‑1 (MQ=60)

                                                                            TGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCA                   >  SRR4302540.7631289/1‑94 (MQ=60)

                                                                            TGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCA                   >  SRR4302540.4339527/1‑94 (MQ=60)

                                                                             GATCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGACCACAGAGTATTGCCACGACCATTTTCCAC                  <  SRR4302540.6832182/94‑1 (MQ=60)

                                                                             GACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCAC                  <  SRR4302540.6220897/94‑1 (MQ=60)

                                                                             GACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCAC                  <  SRR4302540.5847634/94‑1 (MQ=60)

                                                                             GACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCAC                  <  SRR4302540.6220898/94‑1 (MQ=60)

                                                                              ACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACC                 <  SRR4302540.8439666/94‑1 (MQ=60)

                                                                                CAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAG               <  SRR4302540.9000074/94‑1 (MQ=60)

                                                                                CAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAG               <  SRR4302540.9596188/94‑1 (MQ=60)

                                                                                CAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAG               <  SRR4302540.4042280/94‑1 (MQ=60)

                                                                                 AGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGC              <  SRR4302540.8618829/94‑1 (MQ=60)

                                                                                  GCATCGTCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCA             <  SRR4302540.7875682/94‑1 (MQ=60)

                                                                                  GCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCA             <  SRR4302540.7875683/94‑1 (MQ=60)

                                                                                   CATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAA            >  SRR4302540.10095628/1‑94 (MQ=60)

                                                                                   CATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCAACAGAGTATTGCCACGACCATTTTCCACCAGCAA            >  SRR4302540.1864318/1‑94 (MQ=60)

                                                                                    ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT           >  SRR4302540.5222639/1‑94 (MQ=60)

                                                                                    ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT           >  SRR4302540.2440738/1‑94 (MQ=60)

                                                                                    ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT           <  SRR4302540.2639341/94‑1 (MQ=60)

                                                                                    ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT           >  SRR4302540.3862790/1‑94 (MQ=60)

                                                                                    ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT           <  SRR4302540.9544884/94‑1 (MQ=60)

                                                                                    ATCACCACGCTGGGTGATGGTGTAGTTTTCCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT           <  SRR4302540.8946033/94‑1 (MQ=60)

                                                                                     TCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATG          <  SRR4302540.985203/94‑1 (MQ=60)

                                                                                      CATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGA         >  SRR4302540.9102731/1‑94 (MQ=60)

                                                                                      CATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGA         >  SRR4302540.9553136/1‑94 (MQ=60)

                                                                                       ATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCGCCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAA        <  SRR4302540.5994332/94‑1 (MQ=60)

                                                                                       ATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAA        >  SRR4302540.682420/1‑94 (MQ=60)

                                                                                       ATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAA        >  SRR4302540.6627446/1‑94 (MQ=60)

                                                                                       ATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAA        >  SRR4302540.6627444/1‑94 (MQ=60)

                                                                                       ATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAA        <  SRR4302540.2388234/94‑1 (MQ=60)

                                                                                        TCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAG       <  SRR4302540.9339213/94‑1 (MQ=60)

                                                                                        TCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAG       >  SRR4302540.1770442/1‑94 (MQ=60)

                                                                                        TCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAG       <  SRR4302540.80480/94‑1 (MQ=60)

                                                                                            GCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGT   >  SRR4302540.4733496/1‑94 (MQ=60)

                                                                                             CTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGTA  <  SRR4302540.6898581/94‑1 (MQ=60)

                                                                                             CTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGTA  >  SRR4302540.1930392/1‑94 (MQ=60)

                                                                                             CTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGTA  >  SRR4302540.1481044/1‑94 (MQ=60)

AGAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGTA  >  NC_000913/2596394‑2596580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`