Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,596,487	T→C	Q133Q (CAA→CAG)	nlpB ←	lipoprotein‑34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,596,487	0	T	C	100.0%	693.3 / NA	215	Q133Q (CAA→CAG)	nlpB	lipoprotein‑34
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (98/117); total (98/117)

Only 100 of 217 total aligned reads displayed.

GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGC  >  NC_000913/2596397‑2596576

gaTTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTg                                                                                         <  2:4746248/93‑1 (MQ=255)

gaTTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTg                                                                                         >  1:1335412/1‑93 (MQ=255)

 aTTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTgg                                                                                        >  2:5741637/1‑93 (MQ=255)

  tttGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTggg                                                                                       >  2:4508974/1‑93 (MQ=255)

   ttGATAACGACCACTATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGt                                                                                      <  2:4473865/93‑1 (MQ=255)

   ttGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGt                                                                                      >  2:3449576/1‑93 (MQ=255)

      aTAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAt                                                                                   >  1:3600408/1‑93 (MQ=255)

      aTAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAt                                                                                   >  2:1788722/1‑93 (MQ=255)

       tAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATg                                                                                  <  1:5938659/93‑1 (MQ=255)

          cGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGCGATGgtg                                                                               >  1:1081855/1‑93 (MQ=255)

           gACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGgtgt                                                                              <  2:800247/93‑1 (MQ=255)

           gACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGgtgt                                                                              <  1:4931201/93‑1 (MQ=255)

           gACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGgtgt                                                                              >  1:3166248/1‑93 (MQ=255)

           caCCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGgtgt                                                                              <  2:4569924/92‑1 (MQ=255)

             ccACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAg                                                                            >  1:793217/1‑93 (MQ=255)

              cACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGt                                                                           <  1:1646402/93‑1 (MQ=255)

               aCGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGACGGTGTAGtt                                                                          >  1:4121943/1‑93 (MQ=255)

                 gATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGtttt                                                                        >  1:4372461/1‑93 (MQ=255)

                  aTACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGttttt                                                                       <  1:1048953/93‑1 (MQ=255)

                   tACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTc                                                                      >  2:4373263/1‑93 (MQ=255)

                     cTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGc                                                                    >  2:4427206/1‑93 (MQ=255)

                      tGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGcc                                                                   <  2:144124/93‑1 (MQ=255)

                      tGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGcc                                                                   <  1:1546518/93‑1 (MQ=255)

                      tGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGcc                                                                   >  1:1167449/1‑93 (MQ=255)

                      tGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGcc                                                                   <  1:5184245/93‑1 (MQ=255)

                       gCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCt                                                                  >  2:5328089/1‑93 (MQ=255)

                       gCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCt                                                                  >  1:2137964/1‑93 (MQ=255)

                        cTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTg                                                                 <  1:711390/93‑1 (MQ=255)

                         tCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgc                                                                >  2:3015165/1‑93 (MQ=255)

                         tCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgc                                                                <  2:3705621/93‑1 (MQ=255)

                         tCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgc                                                                >  1:5212066/1‑93 (MQ=255)

                          cGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgca                                                               >  2:3948838/1‑93 (MQ=255)

                          cGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgca                                                               >  1:3878163/1‑93 (MQ=255)

                          cGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgca                                                               <  2:1979809/93‑1 (MQ=255)

                          cGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgca                                                               <  1:1822169/93‑1 (MQ=255)

                           gTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgcag                                                              >  1:2813474/1‑93 (MQ=255)

                           gTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgcag                                                              >  1:1254689/1‑93 (MQ=255)

                           gTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgcag                                                              <  2:4524515/93‑1 (MQ=255)

                            tCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgcagc                                                             >  1:863091/1‑93 (MQ=255)

                             cTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTgcagca                                                            <  2:5691717/93‑1 (MQ=255)

                              ttCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCAc                                                           >  2:5172702/1‑93 (MQ=255)

                                 gTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCt                                                        >  1:2295854/1‑93 (MQ=255)

                                 gTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCt                                                        >  1:5232030/1‑93 (MQ=255)

                                  tCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTa                                                       >  2:5276628/1‑93 (MQ=255)

                                   ccAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTaa                                                      >  2:1932/1‑93 (MQ=255)

                                    cAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAac                                                     <  1:4976545/93‑1 (MQ=255)

                                    cAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAac                                                     >  1:2854991/1‑93 (MQ=255)

                                       aCGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAaccac                                                  <  1:4600817/93‑1 (MQ=255)

                                         ggTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCt                                                <  2:1667712/93‑1 (MQ=255)

                                          gTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTg                                               >  1:543070/1‑93 (MQ=255)

                                          gTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTg                                               <  1:5851248/93‑1 (MQ=255)

                                           ttCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGc                                              <  2:128153/93‑1 (MQ=255)

                                           ttCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGc                                              >  1:4415366/1‑93 (MQ=255)

                                              cATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGc                                           <  1:663840/93‑1 (MQ=255)

                                              cATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGc                                           <  1:2960541/93‑1 (MQ=255)

                                               aTTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCAGGATAACCACCAGCAGcc                                          >  2:2181047/1‑93 (MQ=255)

                                                   tACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACag                                      <  2:1583860/93‑1 (MQ=255)

                                                    aCCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACaga                                     <  2:4308133/93‑1 (MQ=255)

                                                    aCCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACaga                                     <  2:3484304/93‑1 (MQ=255)

                                                    aCCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACaga                                     <  2:2108382/93‑1 (MQ=255)

                                                    aCCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACaga                                     <  1:3126803/93‑1 (MQ=255)

                                                     cccAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACagag                                    >  2:5928750/1‑93 (MQ=255)

                                                     cccAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACagag                                    >  2:3273872/1‑93 (MQ=255)

                                                       cAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTa                                  <  1:1805142/93‑1 (MQ=255)

                                                        aaTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAt                                 <  2:4963100/93‑1 (MQ=255)

                                                        aaTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAt                                 <  1:5467976/93‑1 (MQ=255)

                                                         aTCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAtt                                <  2:3201302/93‑1 (MQ=255)

                                                          tCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTg                               >  1:1003370/1‑93 (MQ=255)

                                                          tCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTg                               >  2:2197464/1‑93 (MQ=255)

                                                           cGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGc                              <  2:297213/93‑1 (MQ=255)

                                                             gtggtCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCa                            <  2:4225815/93‑1 (MQ=255)

                                                             gtgctCATTGTCTGACCAGCATCCTCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCa                            <  1:4774836/93‑1 (MQ=255)

                                                               ggtCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACg                          >  2:2648638/1‑93 (MQ=255)

                                                                  cAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGAcc                       >  1:3018738/1‑93 (MQ=255)

                                                                   aGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCa                      >  2:5409674/1‑93 (MQ=255)

                                                                   aGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCa                      >  1:2547311/1‑93 (MQ=255)

                                                                   aGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCa                      >  1:2749112/1‑93 (MQ=255)

                                                                    gtgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAt                     >  1:778304/1‑93 (MQ=255)

                                                                    gtgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAt                     <  1:5318708/93‑1 (MQ=255)

                                                                     tgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtt                    <  2:2340712/93‑1 (MQ=255)

                                                                     tgtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtt                    <  2:4652704/93‑1 (MQ=255)

                                                                      gtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAttt                   <  2:2546133/93‑1 (MQ=255)

                                                                      gtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAttt                   >  2:6155530/1‑93 (MQ=255)

                                                                      gtCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAttt                   >  2:4733734/1‑93 (MQ=255)

                                                                       tCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtttt                  <  2:3680327/93‑1 (MQ=255)

                                                                       tCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtttt                  >  1:1019719/1‑93 (MQ=255)

                                                                       tCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAtttt                  >  1:2410207/1‑93 (MQ=255)

                                                                        cTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTc                 <  1:2338712/93‑1 (MQ=255)

                                                                        cTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTc                 <  1:1227204/93‑1 (MQ=255)

                                                                          gACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTcca               <  1:6103410/93‑1 (MQ=255)

                                                                          gACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTcca               <  1:1763/93‑1 (MQ=255)

                                                                           aCCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTccac              >  1:4305610/1‑93 (MQ=255)

                                                                           aCCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTccac              <  1:2904982/93‑1 (MQ=255)

                                                                             cAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTccacca            >  2:5198550/1‑93 (MQ=255)

                                                                               gCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGc          >  1:4812289/1‑93 (MQ=255)

                                                                                 atcatcACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCaa        <  2:1212417/93‑1 (MQ=255)

                                                                                     tcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGaa    <  2:2311441/93‑1 (MQ=255)

                                                                                     tcaCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGaa    <  2:4215143/93‑1 (MQ=255)

                                                                                       aCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCCGCAATGAAGc  >  1:6023488/1‑93 (MQ=255)

                                                                                       aCGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGc  <  1:3263075/93‑1 (MQ=255)

GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGC  >  NC_000913/2596397‑2596576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGT  >  NC_000913/2596395‑2596579

GAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCT                                                                                             <  SRR4302533.2738470/94‑1 (MQ=60)

GAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCT                                                                                             <  SRR4302533.4043775/94‑1 (MQ=60)

 AGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTG                                                                                            >  SRR4302533.5743977/1‑94 (MQ=60)

 AGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTG                                                                                            <  SRR4302533.1458411/94‑1 (MQ=60)

 AGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTG                                                                                            >  SRR4302533.1343429/1‑94 (MQ=60)

  GATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGG                                                                                           <  SRR4302533.6250422/94‑1 (MQ=60)

    TTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGT                                                                                         <  SRR4302533.4435055/94‑1 (MQ=60)

    TTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGT                                                                                         >  SRR4302533.666333/1‑94 (MQ=60)

     TTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTG                                                                                        >  SRR4302533.1499833/1‑94 (MQ=60)

       GATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGAT                                                                                      >  SRR4302533.3622560/1‑94 (MQ=60)

        ATAACGACCACGATGCTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCATTGTCTGACCAGCATCATCGCGCTGGGTGATG                                                                                     <  SRR4302533.731399/94‑1 (MQ=60)

        ATAACCACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATG                                                                                     <  SRR4302533.122687/94‑1 (MQ=60)

         TAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGG                                                                                    <  SRR4302533.1254790/94‑1 (MQ=60)

         TAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGG                                                                                    <  SRR4302533.5974566/94‑1 (MQ=60)

           ACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTG                                                                                  <  SRR4302533.2339858/94‑1 (MQ=60)

           ACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGCGATGGTG                                                                                  >  SRR4302533.1088237/1‑94 (MQ=60)

            CGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGT                                                                                 >  SRR4302533.3185730/1‑94 (MQ=60)

             GACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTA                                                                                <  SRR4302533.4961062/94‑1 (MQ=60)

              ACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAG                                                                               >  SRR4302533.797885/1‑94 (MQ=60)

                CACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTT                                                                             >  SRR4302533.5282986/1‑94 (MQ=60)

                CACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTT                                                                             <  SRR4302533.1656372/94‑1 (MQ=60)

                CACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGACGGTGTAGTT                                                                             >  SRR4302533.4146968/1‑94 (MQ=60)

                  CGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTT                                                                           >  SRR4302533.4398852/1‑94 (MQ=60)

                  CGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTT                                                                           >  SRR4302533.715035/1‑94 (MQ=60)

                    ATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTC                                                                         <  SRR4302533.1055121/94‑1 (MQ=60)

                       CTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCC                                                                      >  SRR4302533.1174453/1‑94 (MQ=60)

                        TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                     <  SRR4302533.5215542/94‑1 (MQ=60)

                        TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                     <  SRR4302533.1555838/94‑1 (MQ=60)

                        TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCT                                                                     >  SRR4302533.2151148/1‑94 (MQ=60)

                        TGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGACT                                                                     >  SRR4302533.789441/1‑94 (MQ=60)

                          CTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGC                                                                   <  SRR4302533.715591/94‑1 (MQ=60)

                          CTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGC                                                                   >  SRR4302533.1054823/1‑94 (MQ=60)

                          CTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGC                                                                   >  SRR4302533.5243454/1‑94 (MQ=60)

                           TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                  <  SRR4302533.3867632/94‑1 (MQ=60)

                           TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                  <  SRR4302533.4451848/94‑1 (MQ=60)

                           TCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCA                                                                  >  SRR4302533.3901830/1‑94 (MQ=60)

                            CGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAG                                                                 <  SRR4302533.1833300/94‑1 (MQ=60)

                            CGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAG                                                                 <  SRR4302533.3604940/94‑1 (MQ=60)

                            CGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAG                                                                 <  SRR4302533.307698/94‑1 (MQ=60)

                            CGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAG                                                                 >  SRR4302533.1262190/1‑94 (MQ=60)

                            CGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAG                                                                 >  SRR4302533.2830790/1‑94 (MQ=60)

                             GTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGC                                                                >  SRR4302533.868149/1‑94 (MQ=60)

                               CTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCAC                                                              >  SRR4302533.3172101/1‑94 (MQ=60)

                                TTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACG                                                             <  SRR4302533.1742880/94‑1 (MQ=60)

                                  CGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCT                                                           >  SRR4302533.2310071/1‑94 (MQ=60)

                                  CGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCT                                                           >  SRR4302533.5263497/1‑94 (MQ=60)

                                    TCCAGACGGTTCCATTGTCCCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAA                                                         <  SRR4302533.4841185/94‑1 (MQ=60)

                                     CCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAAC                                                        >  SRR4302533.2872559/1‑94 (MQ=60)

                                      CANACGNTTCCATTGTACCCANNCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACC                                                       <  SRR4302533.2964120/94‑1 (MQ=60)

                                      CAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACC                                                       <  SRR4302533.5394809/94‑1 (MQ=60)

                                      CAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACC                                                       <  SRR4302533.5006721/94‑1 (MQ=60)

                                      CAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACC                                                       <  SRR4302533.5363705/94‑1 (MQ=60)

                                      CAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACC                                                       >  SRR4302533.3016289/1‑94 (MQ=60)

                                       AGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCA                                                      <  SRR4302533.4966361/94‑1 (MQ=60)

                                       AGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCA                                                      >  SRR4302533.3200595/1‑94 (MQ=60)

                                       AGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCA                                                      >  SRR4302533.2865861/1‑94 (MQ=60)

                                         GCGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACC                                                    <  SRR4302533.2766804/94‑1 (MQ=60)

                                         ACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACC                                                    <  SRR4302533.4628690/94‑1 (MQ=60)

                                           GGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCTTGCCGCACGCTAACCACCTG                                                  >  SRR4302533.546294/1‑94 (MQ=60)

                                           GGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTG                                                  >  SRR4302533.546293/1‑94 (MQ=60)

                                           GGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTG                                                  >  SRR4302533.1559692/1‑94 (MQ=60)

                                           GGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTG                                                  <  SRR4302533.3953657/94‑1 (MQ=60)

                                            GTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGC                                                 >  SRR4302533.4442029/1‑94 (MQ=60)

                                            GTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGC                                                 <  SRR4302533.5886621/94‑1 (MQ=60)

                                             TTCCATTGTACCCAATCAGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCG                                                <  SRR4302533.5141619/94‑1 (MQ=60)

                                                CATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCC                                             <  SRR4302533.521829/94‑1 (MQ=60)

                                                CATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCC                                             <  SRR4302533.667758/94‑1 (MQ=60)

                                                CATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCC                                             <  SRR4302533.6071340/94‑1 (MQ=60)

                                                CATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCC                                             <  SRR4302533.2978639/94‑1 (MQ=60)

                                                   TGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACA                                          <  SRR4302533.1024997/94‑1 (MQ=60)

                                                   TGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACA                                          >  SRR4302533.2898295/1‑94 (MQ=60)

                                                     TACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGA                                        <  SRR4302533.2568769/94‑1 (MQ=60)

                                                      ACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAG                                       <  SRR4302533.3146024/94‑1 (MQ=60)

                                                         CAATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTAT                                    <  SRR4302533.1816131/94‑1 (MQ=60)

                                                          AATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATT                                   <  SRR4302533.5500931/94‑1 (MQ=60)

                                                           ATCGGTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTG                                  >  SRR4302533.1009234/1‑94 (MQ=60)

                                                               GTGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCAC                              >  SRR4302533.4390706/1‑94 (MQ=60)

                                                               GTGCTCATTGTCTGACCAGCATCCTCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCAC                              <  SRR4302533.4803764/94‑1 (MQ=60)

                                                                TGGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACG                             >  SRR4302533.835396/1‑94 (MQ=60)

                                                                 GGTCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGGTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGA                            >  SRR4302533.4877119/1‑94 (MQ=60)

                                                                   TCAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACC                          >  SRR4302533.3037219/1‑94 (MQ=60)

                                                                    CAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCA                         >  SRR4302533.2562989/1‑94 (MQ=60)

                                                                    CAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCA                         >  SRR4302533.2765967/1‑94 (MQ=60)

                                                                    CAGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCA                         >  SRR4302533.2572944/1‑94 (MQ=60)

                                                                     AGTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCAT                        >  SRR4302533.782884/1‑94 (MQ=60)

                                                                      GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                       <  SRR4302533.2247859/94‑1 (MQ=60)

                                                                      GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                       <  SRR4302533.1070929/94‑1 (MQ=60)

                                                                      GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                       <  SRR4302533.1826178/94‑1 (MQ=60)

                                                                      GTGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATT                       <  SRR4302533.5350582/94‑1 (MQ=60)

                                                                       TGTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTT                      <  SRR4302533.1804576/94‑1 (MQ=60)

                                                                        GTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTT                     >  SRR4302533.1025712/1‑94 (MQ=60)

                                                                        GTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTT                     <  SRR4302533.484834/94‑1 (MQ=60)

                                                                        GTCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTT                     >  SRR4302533.2425093/1‑94 (MQ=60)

                                                                         TCTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTC                    <  SRR4302533.4175330/94‑1 (MQ=60)

                                                                          CTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCC                   <  SRR4302533.1234553/94‑1 (MQ=60)

                                                                          CTGACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCC                   <  SRR4302533.2353194/94‑1 (MQ=60)

                                                                            GACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCAC                 >  SRR4302533.4331517/1‑94 (MQ=60)

                                                                            GACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCAC                 <  SRR4302533.1778/94‑1 (MQ=60)

                                                                            GACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCAC                 <  SRR4302533.6140252/94‑1 (MQ=60)

                                                                             ACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACC                <  SRR4302533.2922824/94‑1 (MQ=60)

                                                                             ACCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACC                >  SRR4302533.1263835/1‑94 (MQ=60)

                                                                              CCAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCA               >  SRR4302533.5895119/1‑94 (MQ=60)

                                                                               CAGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAG              <  SRR4302533.1818953/94‑1 (MQ=60)

                                                                                AGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGC             >  SRR4302533.4841456/1‑94 (MQ=60)

                                                                                AGCATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGC             >  SRR4302533.4781412/1‑94 (MQ=60)

                                                                                  CATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAA           >  SRR4302533.2403489/1‑94 (MQ=60)

                                                                                  CATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAA           <  SRR4302533.4185539/94‑1 (MQ=60)

                                                                                   ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT          <  SRR4302533.524595/94‑1 (MQ=60)

                                                                                   ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT          >  SRR4302533.3083359/1‑94 (MQ=60)

                                                                                   ATCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAAT          >  SRR4302533.2048954/1‑94 (MQ=60)

                                                                                    TCATCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATG         <  SRR4302533.5975031/94‑1 (MQ=60)

                                                                                       TCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAG      <  SRR4302533.1115949/94‑1 (MQ=60)

                                                                                       TCACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAG      <  SRR4302533.2451053/94‑1 (MQ=60)

                                                                                        CACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCCGCAATGAAGC     >  SRR4302533.6059991/1‑94 (MQ=60)

                                                                                        CACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGC     <  SRR4302533.1242225/94‑1 (MQ=60)

                                                                                         ACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCG    >  SRR4302533.5542408/1‑94 (MQ=60)

                                                                                         ACGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCG    <  SRR4302533.3283205/94‑1 (MQ=60)

                                                                                          CGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGG   <  SRR4302533.5358515/94‑1 (MQ=60)

                                                                                          CGCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGG   >  SRR4302533.354696/1‑94 (MQ=60)

                                                                                           GCTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGT  >  SRR4302533.2996390/1‑94 (MQ=60)

GAGATTTGATAACGACCACGATACTGCTCGTCTTCGTCCAGACGGTTCCATTGTACCCAATCGGTGGTCAGTGTCTGACCAGCATCATCACGTTGGGTGATGGTGTAGTTTTTCGCCTGCAGCACGCTAACCACCTGCGGCCACAGAGTATTGCCACGACCATTTTCCACCAGCAATGAAGCGGT  >  NC_000913/2596395‑2596579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`