Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,694,050 A→G Y31C (TAC→TGC)  yfhD → putative periplasmic binding transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,694,0500AG99.0% 657.9 / NA 209Y31C (TAC→TGC) yfhDputative periplasmic binding transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (110/97);  minor base C (0/2);  total (110/99)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

TGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGG  >  NC_000913/2693960‑2694139
                                                                                          |                                                                                         
 gAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCt                                                                                        >  1:5241587/1‑93 (MQ=255)
  aGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTc                                                                                       <  2:4603850/93‑1 (MQ=255)
  aGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTc                                                                                       <  1:1734561/93‑1 (MQ=255)
  aGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTc                                                                                       >  1:624308/1‑93 (MQ=255)
   gCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCg                                                                                      <  1:5524795/93‑1 (MQ=255)
    cACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCgg                                                                                     >  2:5689257/1‑93 (MQ=255)
    cACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCgg                                                                                     >  1:4844137/1‑93 (MQ=255)
      ccATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCg                                                                                   >  2:1655550/1‑93 (MQ=255)
       cATTCATACTCCCCTGACTTATAACGAAATCACCGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGt                                                                                  <  1:4349528/93‑1 (MQ=255)
       cATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGt                                                                                  >  1:5028987/1‑93 (MQ=255)
        aTTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTa                                                                                 >  2:1438106/1‑93 (MQ=255)
         ttCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTaa                                                                                <  1:1638094/93‑1 (MQ=255)
          tCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTaaa                                                                               <  1:1236020/93‑1 (MQ=255)
           cATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTaaaa                                                                              <  1:5716702/93‑1 (MQ=255)
            aTACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAAc                                                                             >  2:2953252/1‑93 (MQ=255)
             tACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACt                                                                            >  1:4743486/1‑93 (MQ=255)
              aCTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTg                                                                           <  1:3939898/93‑1 (MQ=255)
               cTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGa                                                                          <  1:3113407/93‑1 (MQ=255)
               cTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGa                                                                          <  2:4448187/93‑1 (MQ=255)
               cTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGa                                                                          <  2:5195352/93‑1 (MQ=255)
                tCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGaa                                                                         >  2:4813611/1‑93 (MQ=255)
                tCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGaa                                                                         <  2:3134957/93‑1 (MQ=255)
                 ccccTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGaaa                                                                        >  1:2715776/1‑93 (MQ=255)
                 ccccTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGaaa                                                                        <  1:229768/93‑1 (MQ=255)
                  cccTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAg                                                                       >  1:1059990/1‑93 (MQ=255)
                   ccTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGt                                                                      <  1:1596264/93‑1 (MQ=255)
                   ccTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGt                                                                      <  2:6048975/93‑1 (MQ=255)
                     tGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGa                                                                    >  2:1395436/1‑93 (MQ=255)
                       aCTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGAcc                                                                  >  2:4017948/1‑93 (MQ=255)
                       aCTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGAcc                                                                  <  1:5481335/93‑1 (MQ=255)
                       aCTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGAcc                                                                  <  1:4239003/93‑1 (MQ=255)
                        cTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCg                                                                 >  1:2459179/1‑93 (MQ=255)
                         ttATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGt                                                                >  2:2431889/1‑93 (MQ=255)
                         ttATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGt                                                                <  1:3042792/93‑1 (MQ=255)
                         ttATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGt                                                                <  1:578301/93‑1 (MQ=255)
                          tataACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTg                                                               <  2:2317184/93‑1 (MQ=255)
                           ataACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGc                                                              <  1:3595177/93‑1 (MQ=255)
                           ataACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGc                                                              <  1:5353638/93‑1 (MQ=255)
                              aCGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGc                                                           <  1:4483368/93‑1 (MQ=255)
                                    tCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAata                                                     <  1:4076513/93‑1 (MQ=255)
                                    tCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAata                                                     >  2:2075117/1‑93 (MQ=255)
                                    tCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAata                                                     >  1:6005758/1‑93 (MQ=255)
                                     cAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAatat                                                    <  1:4176386/93‑1 (MQ=255)
                                      aaCGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATc                                                   <  1:1306287/93‑1 (MQ=255)
                                      aaCGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATc                                                   <  2:5438325/93‑1 (MQ=255)
                                      aaCGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATc                                                   >  2:823989/1‑93 (MQ=255)
                                         gggAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATcagc                                                <  2:4722226/93‑1 (MQ=255)
                                         gggAAACCTTGTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATcagc                                                <  2:4995063/93‑1 (MQ=255)
                                          ggAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATcagcc                                               <  2:428579/93‑1 (MQ=255)
                                          ggAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATcagcc                                               >  1:1982027/1‑93 (MQ=255)
                                           gAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATcagcca                                              >  2:4212325/1‑93 (MQ=255)
                                            aaaCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATcagccag                                             >  2:2145654/1‑93 (MQ=255)
                                             aaCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATcagccagc                                            >  2:612299/1‑93 (MQ=255)
                                              aCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCt                                           >  2:3435715/1‑93 (MQ=255)
                                              aCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCt                                           >  2:4923350/1‑93 (MQ=255)
                                              aCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCt                                           <  1:5874454/93‑1 (MQ=255)
                                               ccTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAACCAGCTg                                          <  2:5983997/93‑1 (MQ=255)
                                                 ttttGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGtt                                        >  1:6068318/1‑93 (MQ=255)
                                                       ccTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAATCTGAAAGTGACCGTGCGGCAGAATATCATCCAGCTGTTTgacga                                  <  2:1749024/93‑1 (MQ=255)
                                                       ccTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTgacga                                  >  2:4612753/1‑93 (MQ=255)
                                                       ccTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTgacga                                  >  1:1909205/1‑93 (MQ=255)
                                                        cTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTgacgac                                 >  1:3350419/1‑93 (MQ=255)
                                                        cTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTgacgac                                 >  2:3807981/1‑93 (MQ=255)
                                                        cTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTgacgac                                 >  1:5310893/1‑93 (MQ=255)
                                                          ggATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCt                               <  2:2522738/93‑1 (MQ=255)
                                                          ggATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCt                               <  2:2741491/93‑1 (MQ=255)
                                                          ggATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCt                               <  2:5721777/93‑1 (MQ=255)
                                                           gATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCtt                              >  1:2247698/1‑93 (MQ=255)
                                                             ttACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGa                            <  2:4946829/93‑1 (MQ=255)
                                                               aCGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATa                          <  2:1569274/93‑1 (MQ=255)
                                                                 gAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAAt                        >  2:5969161/1‑93 (MQ=255)
                                                                 gAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAAt                        <  2:5316469/93‑1 (MQ=255)
                                                                   aCTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATgg                      >  2:3332624/1‑93 (MQ=255)
                                                                   aCTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATgg                      >  1:2862119/1‑93 (MQ=255)
                                                                   aCTGGCGAAACAGTTTGCCGATTCCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATgg                      <  2:5225496/93‑1 (MQ=255)
                                                                     tGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTa                    <  1:350720/93‑1 (MQ=255)
                                                                      ggCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTaa                   >  1:5858989/1‑93 (MQ=255)
                                                                        cGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACg                 <  2:2154276/93‑1 (MQ=255)
                                                                         gAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGc                >  1:240812/1‑93 (MQ=255)
                                                                           aaCAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCg              >  1:4582724/1‑93 (MQ=255)
                                                                           aaCAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCg              <  1:1822981/93‑1 (MQ=255)
                                                                           aaCAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCg              >  2:5033289/1‑93 (MQ=255)
                                                                           aaCAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCg              >  2:337484/1‑93 (MQ=255)
                                                                            aCAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGa             >  1:210562/1‑93 (MQ=255)
                                                                               gTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACct          >  1:3458074/1‑93 (MQ=255)
                                                                               gTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACct          >  1:3221240/1‑93 (MQ=255)
                                                                               gTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACct          >  2:2765906/1‑93 (MQ=255)
                                                                                 ttGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACctgc        <  1:2942256/93‑1 (MQ=255)
                                                                                 ttGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACctgc        <  1:825522/93‑1 (MQ=255)
                                                                                  tGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACctgct       >  1:249169/1‑93 (MQ=255)
                                                                                   tccGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACctgctg      <  2:4856440/92‑1 (MQ=255)
                                                                                   gCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACctgctg      <  1:3868208/93‑1 (MQ=255)
                                                                                   gCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACctgctg      >  1:2624/1‑93 (MQ=255)
                                                                                   accGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACctgctg      <  2:6186784/92‑1 (MQ=255)
                                                                                    ccGATTGCCTCGTCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTGGACTACCTTGATAATGGTAACGCCGACCTGCTgg     <  2:697039/93‑1 (MQ=255)
                                                                                    ccGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTgg     <  2:2700644/93‑1 (MQ=255)
                                                                                    ccGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTgg     <  1:2644447/93‑1 (MQ=255)
                                                                                     cGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTggg    >  2:1206232/1‑92 (MQ=255)
                                                                                     cGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTggc    <  1:673798/93‑1 (MQ=255)
                                                                                      gATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGGTAATGGTAACGCCGACCTGCTggcg   >  1:1577746/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGG  >  NC_000913/2693960‑2694139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGG  >  NC_000913/2693957‑2694143
                                                                                             |                                                                                             
GTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTG                                                                                               >  SRR4302533.2129249/1‑94 (MQ=60)
GTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTG                                                                                               <  SRR4302533.5308337/94‑1 (MQ=60)
GTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTG                                                                                               <  SRR4302533.2880941/94‑1 (MQ=60)
GTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTG                                                                                               <  SRR4302533.5840538/94‑1 (MQ=60)
  GTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCC                                                                                             >  SRR4302533.3120590/1‑94 (MQ=60)
   TGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCT                                                                                            >  SRR4302533.5273095/1‑94 (MQ=60)
    GAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTC                                                                                           >  SRR4302533.627972/1‑94 (MQ=60)
     AGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCG                                                                                          >  SRR4302533.39419/1‑94 (MQ=60)
     AGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCG                                                                                          <  SRR4302533.1745086/94‑1 (MQ=60)
      GCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGG                                                                                         <  SRR4302533.5558148/94‑1 (MQ=60)
      GCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGG                                                                                         >  SRR4302533.4873496/1‑94 (MQ=60)
       CACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGC                                                                                        >  SRR4302533.133716/1‑94 (MQ=60)
        ACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCG                                                                                       <  SRR4302533.2307734/94‑1 (MQ=60)
         CCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGT                                                                                      >  SRR4302533.5059435/1‑94 (MQ=60)
         CCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGT                                                                                      >  SRR4302533.3306967/1‑94 (MQ=60)
         CCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGGTTGCCGATTGCCTCCGCGT                                                                                      >  SRR4302533.5495293/1‑94 (MQ=60)
          CATTCATACTCCCCTGTCTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTA                                                                                     <  SRR4302533.1966584/94‑1 (MQ=60)
          CATTCATACTCCCCTGACTTATAACGAAATCACCGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTA                                                                                     <  SRR4302533.4375750/94‑1 (MQ=60)
          CATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTA                                                                                     >  SRR4302533.1299997/1‑94 (MQ=60)
            TTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAA                                                                                   <  SRR4302533.1648011/94‑1 (MQ=60)
             TCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAA                                                                                  <  SRR4302533.1243408/94‑1 (MQ=60)
              CATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAAC                                                                                 <  SRR4302533.5751227/94‑1 (MQ=60)
               ATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACT                                                                                >  SRR4302533.4772242/1‑94 (MQ=60)
                 ACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGA                                                                              <  SRR4302533.3963942/94‑1 (MQ=60)
                  CTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAA                                                                             <  SRR4302533.3132554/94‑1 (MQ=60)
                   TCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAA                                                                            >  SRR4302533.2732450/1‑94 (MQ=60)
                    CCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAG                                                                           >  SRR4302533.1066222/1‑94 (MQ=60)
                    CCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAG                                                                           <  SRR4302533.231192/94‑1 (MQ=60)
                      CGTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTGTGCCGATTGCCTCGGCGTAAAACTGAAAGTG                                                                         <  SRR4302533.1595223/94‑1 (MQ=60)
                      CCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTG                                                                         <  SRR4302533.1605930/94‑1 (MQ=60)
                      CCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTG                                                                         >  SRR4302533.2459112/1‑94 (MQ=60)
                       CTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGA                                                                        >  SRR4302533.455147/1‑94 (MQ=60)
                        TGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGAC                                                                       >  SRR4302533.4334783/1‑94 (MQ=60)
                         GACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACC                                                                      <  SRR4302533.347614/94‑1 (MQ=60)
                          ACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCG                                                                     <  SRR4302533.5767819/94‑1 (MQ=60)
                          ACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCG                                                                     >  SRR4302533.2474353/1‑94 (MQ=60)
                          ACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCG                                                                     <  SRR4302533.5514381/94‑1 (MQ=60)
                          ACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCG                                                                     <  SRR4302533.4264606/94‑1 (MQ=60)
                           CTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGT                                                                    <  SRR4302533.4686750/94‑1 (MQ=60)
                            TTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTG                                                                   <  SRR4302533.581711/94‑1 (MQ=60)
                            TTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTG                                                                   <  SRR4302533.5919930/94‑1 (MQ=60)
                            TTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTG                                                                   <  SRR4302533.3061418/94‑1 (MQ=60)
                            TTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTG                                                                   <  SRR4302533.2936895/94‑1 (MQ=60)
                              ATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCG                                                                 <  SRR4302533.3617297/94‑1 (MQ=60)
                              ATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCG                                                                 <  SRR4302533.5385769/94‑1 (MQ=60)
                              ATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCG                                                                 >  SRR4302533.763604/1‑94 (MQ=60)
                                AACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGA                                                               >  SRR4302533.5531568/1‑94 (MQ=60)
                                 ACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCA                                                              <  SRR4302533.4510516/94‑1 (MQ=60)
                                 ACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCA                                                              <  SRR4302533.536129/94‑1 (MQ=60)
                                   GAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGA                                                            >  SRR4302533.4100730/1‑94 (MQ=60)
                                      ATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATA                                                         >  SRR4302533.6042147/1‑94 (MQ=60)
                                      ATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATA                                                         <  SRR4302533.4847567/94‑1 (MQ=60)
                                       TCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATAT                                                        <  SRR4302533.4101327/94‑1 (MQ=60)
                                        CACCGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTCCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATC                                                       <  SRR4302533.2766046/94‑1 (MQ=60)
                                        CAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATC                                                       >  SRR4302533.4934624/1‑94 (MQ=60)
                                        CAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATC                                                       >  SRR4302533.5907320/1‑94 (MQ=60)
                                        CAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATC                                                       <  SRR4302533.4201690/94‑1 (MQ=60)
                                         AACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCA                                                      <  SRR4302533.326414/94‑1 (MQ=60)
                                         AACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCA                                                      >  SRR4302533.5855924/1‑94 (MQ=60)
                                         AACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCA                                                      <  SRR4302533.1314134/94‑1 (MQ=60)
                                          ACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAG                                                     <  SRR4302533.5695273/94‑1 (MQ=60)
                                           CGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGC                                                    <  SRR4302533.2641761/94‑1 (MQ=60)
                                           CGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGC                                                    >  SRR4302533.6063897/1‑94 (MQ=60)
                                           CGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCATGCGGCAGAATATCAGC                                                    >  SRR4302533.2090413/1‑94 (MQ=60)
                                            GGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCCGCC                                                   >  SRR4302533.3184670/1‑94 (MQ=60)
                                            GGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCC                                                   >  SRR4302533.1994159/1‑94 (MQ=60)
                                             GGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCA                                                  <  SRR4302533.1158864/94‑1 (MQ=60)
                                              GAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAG                                                 >  SRR4302533.6113374/1‑94 (MQ=60)
                                               AAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGC                                                <  SRR4302533.3996210/94‑1 (MQ=60)
                                               AAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGC                                                >  SRR4302533.5941064/1‑94 (MQ=60)
                                                 ACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTG                                              <  SRR4302533.5909962/94‑1 (MQ=60)
                                                 ACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTG                                              <  SRR4302533.4157730/94‑1 (MQ=60)
                                                   CTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTT                                            >  SRR4302533.6105036/1‑94 (MQ=60)
                                                      TTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGA                                         <  SRR4302533.1659882/94‑1 (MQ=60)
                                                       TGGCCGGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGAC                                        <  SRR4302533.2357333/94‑1 (MQ=60)
                                                        GGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACG                                       >  SRR4302533.3733088/1‑94 (MQ=60)
                                                         GCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGA                                      >  SRR4302533.1920851/1‑94 (MQ=60)
                                                          CCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGAC                                     >  SRR4302533.5342720/1‑94 (MQ=60)
                                                          CCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGAC                                     >  SRR4302533.2968772/1‑94 (MQ=60)
                                                          CCTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGAC                                     >  SRR4302533.3371116/1‑94 (MQ=60)
                                                           CTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACC                                    <  SRR4302533.1849830/94‑1 (MQ=60)
                                                           CTGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACC                                    >  SRR4302533.2662160/1‑94 (MQ=60)
                                                            TGGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCT                                   <  SRR4302533.661312/94‑1 (MQ=60)
                                                             GGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTT                                  <  SRR4302533.2185798/94‑1 (MQ=60)
                                                             GGATTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTT                                  >  SRR4302533.2261587/1‑94 (MQ=60)
                                                                TTACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGAT                               >  SRR4302533.1617810/1‑94 (MQ=60)
                                                                 TACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATA                              <  SRR4302533.3604328/94‑1 (MQ=60)
                                                                 TACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATA                              >  SRR4302533.2698882/1‑94 (MQ=60)
                                                                  ACGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAA                             <  SRR4302533.909513/94‑1 (MQ=60)
                                                                   CGAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGAGACCGTGCGGCAGAATATCAGCCAGCTGTTTGATGACCTTGATAAT                            >  SRR4302533.4675031/1‑94 (MQ=60)
                                                                    GAACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATG                           >  SRR4302533.5410982/1‑94 (MQ=60)
                                                                    GAACTGGCGAAACAGTTTGCCGACTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATG                           >  SRR4302533.4443543/1‑94 (MQ=60)
                                                                     AACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGG                          <  SRR4302533.5060914/94‑1 (MQ=60)
                                                                     AACTGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGG                          >  SRR4302533.2879725/1‑94 (MQ=60)
                                                                        TGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAA                       <  SRR4302533.352856/94‑1 (MQ=60)
                                                                        TGGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAA                       >  SRR4302533.5894408/1‑94 (MQ=60)
                                                                         GGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAAC                      <  SRR4302533.167399/94‑1 (MQ=60)
                                                                         GGCGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAAC                      <  SRR4302533.2545587/94‑1 (MQ=60)
                                                                           CGAAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGC                    >  SRR4302533.242306/1‑94 (MQ=60)
                                                                             AAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCG                  >  SRR4302533.4211204/1‑94 (MQ=60)
                                                                             AAACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCG                  >  SRR4302533.4610490/1‑94 (MQ=60)
                                                                              AACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGA                 <  SRR4302533.1834117/94‑1 (MQ=60)
                                                                              AACAGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGA                 >  SRR4302533.211890/1‑94 (MQ=60)
                                                                                 AGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCT              >  SRR4302533.3479421/1‑94 (MQ=60)
                                                                                 AGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCT              >  SRR4302533.1800437/1‑94 (MQ=60)
                                                                                 AGTTTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCT              >  SRR4302533.3241102/1‑94 (MQ=60)
                                                                                    TTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCT           >  SRR4302533.250707/1‑94 (MQ=60)
                                                                                    TTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCT           <  SRR4302533.2960222/94‑1 (MQ=60)
                                                                                    TTGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCT           <  SRR4302533.830366/94‑1 (MQ=60)
                                                                                     TGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTG          >  SRR4302533.2644/1‑94 (MQ=60)
                                                                                     TGCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTG          <  SRR4302533.2275662/94‑1 (MQ=60)
                                                                                      GCCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGG         <  SRR4302533.3891824/94‑1 (MQ=60)
                                                                                       CCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGC        <  SRR4302533.2660696/94‑1 (MQ=60)
                                                                                       CCGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGC        >  SRR4302533.5287409/1‑94 (MQ=60)
                                                                                        CGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGGTAATGGTAACGCCGACCTGCTGGCG       >  SRR4302533.1587246/1‑94 (MQ=60)
                                                                                        CGATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCG       <  SRR4302533.677774/94‑1 (MQ=60)
                                                                                         GATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGG      >  SRR4302533.352332/1‑94 (MQ=60)
                                                                                         GATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGG      >  SRR4302533.249367/1‑94 (MQ=60)
                                                                                          ATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGC     >  SRR4302533.981961/1‑94 (MQ=60)
                                                                                          ATTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGC     >  SRR4302533.408048/1‑94 (MQ=60)
                                                                                           TTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCA    <  SRR4302533.5819834/94‑1 (MQ=60)
                                                                                           TTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCA    >  SRR4302533.744765/1‑94 (MQ=60)
                                                                                           TTGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCA    <  SRR4302533.3772462/94‑1 (MQ=60)
                                                                                            TGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAG   <  SRR4302533.3142185/94‑1 (MQ=60)
                                                                                            TGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAG   <  SRR4302533.1227610/94‑1 (MQ=60)
                                                                                            TGCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAG   >  SRR4302533.946620/1‑94 (MQ=60)
                                                                                             GCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGG  >  SRR4302533.57218/1‑94 (MQ=60)
                                                                                             GCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGG  >  SRR4302533.3633820/1‑94 (MQ=60)
                                                                                             GCCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGG  <  SRR4302533.4377080/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGG  >  NC_000913/2693957‑2694143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑