Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,020	+G	intergenic (‑144/+65)	ypjA ← / ← b2648	putative ATP‑binding component of a transport system/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,019	1	.	G	99.5%	629.3 / ‑3.8	207	intergenic (‑143/+66)	ypjA/b2648	putative ATP‑binding component of a transport system/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (92/114); total (92/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 218 total aligned reads displayed.

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG  >  NC_000913/2780927‑2781106

ggCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                                          <  2:3081503/93‑1 (MQ=255)

ggCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                                          <  1:2107503/93‑1 (MQ=255)

 gCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                                        >  2:2731312/1‑93 (MQ=255)

 gCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                                        >  2:5620752/1‑93 (MQ=255)

  cATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑gg                                                                                       <  1:3705166/93‑2 (MQ=255)

  cATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑gg                                                                                       <  1:2871382/93‑2 (MQ=255)

        ttAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTg                                                                                  <  1:2500375/93‑1 (MQ=255)

           aaCGCAGTGCGCTGGAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGGATTCTTTTCTTTCGACAATGAGGCCGTTGGCa                                                                               >  2:3850821/1‑93 (MQ=25)

            aCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                                              <  2:4575702/93‑1 (MQ=255)

            aCGCAGTGCGCCGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                                              >  1:5017604/1‑93 (MQ=25)

             cGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaa                                                                             >  2:2462659/1‑93 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            >  2:4341708/1‑93 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            <  2:1603737/93‑1 (MQ=25)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            <  2:4247698/93‑1 (MQ=255)

                aGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaata                                                                          <  2:1247923/93‑1 (MQ=25)

                  tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaa                                                                        >  2:653925/1‑93 (MQ=255)

                  tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaa                                                                        >  2:2671120/1‑93 (MQ=255)

                    cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                      <  2:2041026/93‑1 (MQ=25)

                    cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                      <  2:2914097/93‑1 (MQ=25)

                    cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                      <  2:6209086/93‑1 (MQ=25)

                    cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                      <  2:2133104/93‑1 (MQ=25)

                      cTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                                    <  1:3088293/93‑1 (MQ=255)

                       tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                   <  1:2310506/93‑1 (MQ=25)

                       tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                   <  2:5830319/93‑1 (MQ=255)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  <  2:1058599/93‑1 (MQ=25)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  >  2:1116306/1‑93 (MQ=255)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  >  2:1224935/1‑93 (MQ=255)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  <  2:3502016/93‑1 (MQ=255)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  >  2:5975990/1‑93 (MQ=255)

                         aaGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAttt                                                                 <  2:727093/93‑1 (MQ=37)

                          aGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTa                                                                <  1:2214505/93‑1 (MQ=255)

                           gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAc                                                               <  2:5660656/93‑1 (MQ=255)

                           gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAc                                                               <  2:472823/93‑1 (MQ=37)

                              tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAGATa                                                            >  2:4854503/1‑93 (MQ=25)

                              tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                                            <  2:532109/93‑1 (MQ=255)

                                gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                          >  2:5022338/1‑93 (MQ=255)

                                gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                          <  1:5264290/93‑1 (MQ=37)

                                 ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                         <  2:865973/93‑1 (MQ=37)

                                 ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                         >  1:677372/1‑93 (MQ=255)

                                 ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                         <  1:3304050/93‑1 (MQ=255)

                                  tGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCg                                                        >  2:1907865/1‑93 (MQ=255)

                                      tAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTc                                                    >  1:1521816/1‑93 (MQ=255)

                                       aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCt                                                   <  1:2050681/93‑1 (MQ=255)

                                       aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCt                                                   <  1:843248/93‑1 (MQ=38)

                                        aaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtg                                                  <  2:3782765/93‑1 (MQ=255)

                                         aaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtga                                                 <  1:265333/93‑1 (MQ=255)

                                          aaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgat                                                >  1:4316599/1‑93 (MQ=255)

                                           aaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatg                                               >  1:6128251/1‑93 (MQ=255)

                                           aaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatg                                               >  1:4554560/1‑93 (MQ=255)

                                             gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                                             >  2:3367547/1‑93 (MQ=255)

                                             gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                                             <  1:3518783/93‑1 (MQ=255)

                                               cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAata                                           >  2:447951/1‑93 (MQ=255)

                                               cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAata                                           >  1:3802559/1‑93 (MQ=255)

                                                aaTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAatat                                          >  1:4932969/1‑93 (MQ=255)

                                                aaTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAatat                                          >  1:4739123/1‑93 (MQ=255)

                                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                      >  1:991704/1‑93 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     <  1:4460326/93‑1 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     <  1:1356658/93‑1 (MQ=25)

                                                      ttCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTg                                    <  2:4926082/93‑1 (MQ=255)

                                                      ttCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTg                                    >  1:4844300/1‑93 (MQ=255)

                                                       tCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGc                                   <  1:5397398/93‑1 (MQ=255)

                                                          ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                <  2:3005685/93‑1 (MQ=255)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                            >  1:1261934/1‑93 (MQ=18)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                            >  2:1577653/1‑93 (MQ=18)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                            >  1:2021628/1‑93 (MQ=255)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                            >  1:3666261/1‑93 (MQ=255)

                                                               tACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaa                           >  1:4698905/1‑93 (MQ=18)

                                                                  gggCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaaaa                        <  2:4106103/93‑1 (MQ=255)

                                                                   ggCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAt                       <  1:1721235/93‑1 (MQ=18)

                                                                    gCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTCCATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc                      <  1:3374605/93‑1 (MQ=18)

                                                                    gCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc                      >  2:1340057/1‑93 (MQ=255)

                                                                       tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                   >  1:5461867/1‑93 (MQ=17)

                                                                       tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                   <  1:3249928/93‑1 (MQ=255)

                                                                       tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                   <  1:1642695/93‑1 (MQ=255)

                                                                        aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                  >  2:6050874/1‑93 (MQ=255)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 >  2:3265787/1‑93 (MQ=17)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 <  1:3205757/93‑1 (MQ=255)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 >  2:469710/1‑93 (MQ=17)

                                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACaa                >  1:4902400/1‑93 (MQ=255)

                                                                             tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa             <  2:3754001/93‑1 (MQ=255)

                                                                             tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa             <  1:3941913/93‑1 (MQ=255)

                                                                               tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt           >  2:4295214/1‑93 (MQ=255)

                                                                               tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt           >  1:421905/1‑93 (MQ=17)

                                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt          <  2:2383404/93‑1 (MQ=255)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         <  1:1588418/93‑1 (MQ=17)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         <  2:3989696/93‑1 (MQ=14)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         <  2:2190780/93‑1 (MQ=255)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         <  2:2110603/93‑1 (MQ=17)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         <  2:2034592/93‑1 (MQ=255)

                                                                                  ttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTg        <  1:5916706/93‑1 (MQ=255)

                                                                                  ttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTg        <  2:3862599/93‑1 (MQ=255)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  1:525234/93‑1 (MQ=255)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  2:1935172/93‑1 (MQ=255)

                                                                                    cGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgag      <  1:4075865/93‑1 (MQ=255)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAANAATCACACAATAACTTTgaga     >  1:2388101/1‑93 (MQ=17)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga     >  1:1946708/1‑93 (MQ=18)

                                                                                      aCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAt    <  2:5507851/93‑1 (MQ=255)

                                                                                       cAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAACAACTTTGAGATc   <  1:3405131/93‑1 (MQ=18)

                                                                                        aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCg  >  1:5804617/1‑93 (MQ=18)

                                                                                        aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCg  >  1:1071791/1‑93 (MQ=18)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG  >  NC_000913/2780927‑2781106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G‑CCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTG  >  NC_000913/2780927‑2781113

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G                                                                                                <  SRR4302533.2120504/94‑1 (MQ=60)

  CATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G‑GC                                                                                             <  SRR4302533.3727923/94‑1 (MQ=60)

  CATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G‑GC                                                                                             <  SRR4302533.2889049/94‑1 (MQ=60)

  CATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G‑GC                                                                                             >  SRR4302533.168914/1‑94 (MQ=60)

      TTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTT                                                                                          <  SRR4302533.2725608/94‑1 (MQ=60)

       TGTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTG                                                                                         <  SRR4302533.2642808/94‑1 (MQ=60)

        TTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑Ggccgttgg                                                                                        >  SRR4302533.3517156/1‑86 (MQ=60)

        TTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑Ggccgttgg                                                                                        <  SRR4302533.2515798/94‑9 (MQ=60)

           AACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAA                                                                                     >  SRR4302533.5908177/1‑94 (MQ=60)

           AACGCAGTGCGCCGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAA                                                                                     >  SRR4302533.5047998/1‑94 (MQ=60)

              GCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAAT                                                                                  <  SRR4302533.453571/94‑1 (MQ=60)

               CAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATA                                                                                 <  SRR4302533.2185382/94‑1 (MQ=60)

                 GTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAA                                                                               <  SRR4302533.82878/94‑1 (MQ=60)

                  TGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAA                                                                              <  SRR4302533.1733544/94‑1 (MQ=60)

                   GCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAAT                                                                             <  SRR4302533.5034183/94‑1 (MQ=60)

                     GCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGA                                                                           <  SRR4302533.6240804/94‑1 (MQ=60)

                     GCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGA                                                                           <  SRR4302533.2914812/94‑1 (MQ=60)

                      CTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATCCGGGCGTATTCTTTTCTTTCGACAATGA‑GTCCGTTGGCAAAATAAAATGAT                                                                          <  SRR4302533.1304186/94‑1 (MQ=60)

                      CTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGAT                                                                          <  SRR4302533.3107279/94‑1 (MQ=60)

                       TGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATT                                                                         <  SRR4302533.2324820/94‑1 (MQ=60)

                         AAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTA                                                                       >  SRR4302533.3377003/1‑94 (MQ=60)

                          AGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTAC                                                                      <  SRR4302533.2228171/94‑1 (MQ=60)

                             GTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATA                                                                   <  SRR4302533.985142/94‑1 (MQ=60)

                               GGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAAT                                                                 <  SRR4302533.4620800/94‑1 (MQ=60)

                                GTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATC                                                                <  SRR4302533.3537974/94‑1 (MQ=60)

                                GTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATC                                                                >  SRR4302533.681376/1‑94 (MQ=60)

                                GTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATC                                                                <  SRR4302533.5295886/94‑1 (MQ=60)

                                 TTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCG                                                               <  SRR4302533.5274203/94‑1 (MQ=60)

                                 TTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCG                                                               <  SRR4302533.3324454/94‑1 (MQ=60)

                                 TTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCG                                                               <  SRR4302533.199394/94‑1 (MQ=60)

                                   GGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTT                                                             <  SRR4302533.2096431/94‑1 (MQ=60)

                                    GATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTT                                                            <  SRR4302533.5206737/94‑1 (MQ=60)

                                    GATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTT                                                            >  SRR4302533.3704670/1‑94 (MQ=60)

                                     ATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTC                                                           >  SRR4302533.1530978/1‑94 (MQ=60)

                                       AAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTG                                                         <  SRR4302533.2063332/94‑1 (MQ=60)

                                       AAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTG                                                         <  SRR4302533.848179/94‑1 (MQ=60)

                                         AAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGAT                                                       >  SRR4302533.4814803/1‑94 (MQ=60)

                                         AAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGAT                                                       >  SRR4302533.4342592/1‑94 (MQ=60)

                                         AAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGAT                                                       >  SRR4302533.1731620/1‑94 (MQ=60)

                                         AAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGAT                                                       <  SRR4302533.266947/94‑1 (MQ=60)

                                          AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                      >  SRR4302533.6165184/1‑94 (MQ=60)

                                          AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                      >  SRR4302533.4756535/1‑94 (MQ=60)

                                          AAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATG                                                      >  SRR4302533.4582181/1‑94 (MQ=60)

                                             GTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAAT                                                   <  SRR4302533.3540476/94‑1 (MQ=60)

                                              TCAATCCATTCAGGGAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATA                                                  >  SRR4302533.3414912/1‑94 (MQ=60)

                                              TCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATA                                                  <  SRR4302533.3237212/94‑1 (MQ=60)

                                              TCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATA                                                  >  SRR4302533.3825828/1‑94 (MQ=60)

                                               CAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGTCAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAT                                                 <  SRR4302533.4281617/94‑1 (MQ=60)

                                               CAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAT                                                 >  SRR4302533.4962839/1‑94 (MQ=60)

                                               CAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAT                                                 >  SRR4302533.4767858/1‑94 (MQ=60)

                                                AATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATC                                                <  SRR4302533.5491815/94‑1 (MQ=60)

                                                   CCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTC                                             >  SRR4302533.997501/1‑94 (MQ=60)

                                                   CCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTC                                             >  SRR4302533.1412105/1‑94 (MQ=60)

                                                    CATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCT                                            <  SRR4302533.5323587/94‑1 (MQ=60)

                                                     ATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTG                                           <  SRR4302533.4487307/94‑1 (MQ=60)

                                                     ATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTG                                           >  SRR4302533.4873663/1‑94 (MQ=60)

                                                     ATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTG                                           <  SRR4302533.1364811/94‑1 (MQ=60)

                                                      TTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGC                                          <  SRR4302533.407573/94‑1 (MQ=60)

                                                       TCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCT                                         <  SRR4302533.5429831/94‑1 (MQ=60)

                                                         AGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCA                                       >  SRR4302533.4364034/1‑94 (MQ=60)

                                                             AATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATA                                   >  SRR4302533.2034078/1‑94 (MQ=60)

                                                             AATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATA                                   <  SRR4302533.1594393/94‑1 (MQ=60)

                                                             AATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATA                                   >  SRR4302533.3688798/1‑94 (MQ=60)

                                                             AATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATA                                   >  SRR4302533.1269480/1‑94 (MQ=60)

                                                              ATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAA                                  >  SRR4302533.4727389/1‑94 (MQ=60)

                                                              ATACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAA                                  <  SRR4302533.1155776/94‑1 (MQ=60)

                                                               TACGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAA                                 >  SRR4302533.2034613/1‑94 (MQ=60)

                                                                 CGGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAA                               <  SRR4302533.6044399/94‑1 (MQ=60)

                                                                  GGGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAT                              <  SRR4302533.2929848/94‑1 (MQ=60)

                                                                   GGCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATC                             <  SRR4302533.1731674/94‑1 (MQ=60)

                                                                    GCGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTCCATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCA                            <  SRR4302533.3395442/94‑1 (MQ=60)

                                                                     CGTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCAC                           >  SRR4302533.1652505/1‑94 (MQ=60)

                                                                      GTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACA                          >  SRR4302533.5494772/1‑94 (MQ=60)

                                                                      GTATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACA                          <  SRR4302533.4759807/94‑1 (MQ=60)

                                                                       TATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACAC                         <  SRR4302533.1652643/94‑1 (MQ=59)

                                                                       TATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACAC                         <  SRR4302533.3269967/94‑1 (MQ=59)

                                                                       TATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACAC                         >  SRR4302533.401023/1‑94 (MQ=59)

                                                                        ATTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACA                        <  SRR4302533.5290235/94‑1 (MQ=56)

                                                                         TTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                       >  SRR4302533.4932105/1‑94 (MQ=54)

                                                                         TTCTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAA                       <  SRR4302533.3225494/94‑1 (MQ=54)

                                                                           CTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATA                     <  SRR4302533.2340172/94‑1 (MQ=49)

                                                                           CTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATA                     <  SRR4302533.2881638/94‑1 (MQ=49)

                                                                           CTTTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATA                     >  SRR4302533.3114415/1‑94 (MQ=49)

                                                                             TTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAC                   <  SRR4302533.3965965/94‑1 (MQ=47)

                                                                             TTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAC                   >  SRR4302533.2250440/1‑94 (MQ=47)

                                                                             TTTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAC                   >  SRR4302533.420793/1‑94 (MQ=47)

                                                                              TTCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACT                  >  SRR4302533.424429/1‑94 (MQ=47)

                                                                               TCTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTT                 >  SRR4302533.3308143/1‑94 (MQ=47)

                                                                                CTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTT                <  SRR4302533.6151559/94‑1 (MQ=49)

                                                                                CTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTT                <  SRR4302533.2255312/94‑1 (MQ=49)

                                                                                CTTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTT                >  SRR4302533.2416688/1‑94 (MQ=49)

                                                                                 TTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTG               <  SRR4302533.1598021/94‑1 (MQ=52)

                                                                                 TTTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTG               <  SRR4302533.3937602/94‑1 (MQ=52)

                                                                                  TTCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGA              <  SRR4302533.5952465/94‑1 (MQ=54)

                                                                                   TCGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAG             <  SRR4302533.528346/94‑1 (MQ=56)

                                                                                    CGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAANAATCACACAATAACTTTGAGA            >  SRR4302533.2402855/1‑94 (MQ=60)

                                                                                    CGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGA            >  SRR4302533.1958578/1‑94 (MQ=59)

                                                                                    CGACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGA            <  SRR4302533.4100676/94‑1 (MQ=59)

                                                                                     GACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAT           <  SRR4302533.4955865/94‑1 (MQ=60)

                                                                                     GACAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAT           >  SRR4302533.3793823/1‑94 (MQ=60)

                                                                                       CAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG         >  SRR4302533.4889194/1‑94 (MQ=60)

                                                                                       CAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG         >  SRR4302533.1078106/1‑94 (MQ=60)

                                                                                       CAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG         >  SRR4302533.5830229/1‑94 (MQ=60)

                                                                                       CAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG         >  SRR4302533.5839671/1‑94 (MQ=60)

                                                                                       CAATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAACAACTTTGAGATCG         <  SRR4302533.3426162/94‑1 (MQ=60)

                                                                                        AATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGC        >  SRR4302533.12621/1‑94 (MQ=60)

                                                                                        AATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGC        <  SRR4302533.3557114/94‑1 (MQ=60)

                                                                                         ATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCA       >  SRR4302533.3976718/1‑94 (MQ=60)

                                                                                         ATGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCA       <  SRR4302533.4620250/94‑1 (MQ=60)

                                                                                          TGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAG      <  SRR4302533.1840806/94‑1 (MQ=60)

                                                                                          TGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAG      <  SRR4302533.5315663/94‑1 (MQ=60)

                                                                                          TGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAG      <  SRR4302533.2423038/94‑1 (MQ=60)

                                                                                          TGAGG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAG      <  SRR4302533.2689468/94‑1 (MQ=60)

                                                                                            agG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAT    <  SRR4302533.5592837/92‑1 (MQ=60)

                                                                                            agG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAT    <  SRR4302533.4913545/92‑1 (MQ=60)

                                                                                            agG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAT    <  SRR4302533.1265553/92‑1 (MQ=60)

                                                                                            agG‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAT    >  SRR4302533.950886/3‑94 (MQ=60)

                                                                                              G‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTG  >  SRR4302533.2677381/1‑94 (MQ=60)

                                                                                              G‑CCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTG  <  SRR4302533.2812051/94‑1 (MQ=60)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑G‑CCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTG  >  NC_000913/2780927‑2781113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`