Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,818,916 C→T R373H (CGC→CAC)  alaS ← alanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,9160CT99.6% 999.3 / ‑5.6 273R373H (CGC→CAC) alaSalanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (142/130);  total (142/131)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

Only 100 of 277 total aligned reads displayed.

CCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGCGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAG  >  NC_000913/2818826‑2819005
                                                                                          |                                                                                         
ccATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCgtgc                                                                                         >  2:2277924/1‑93 (MQ=255)
    aGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCa                                                                                     <  1:5877727/93‑1 (MQ=255)
      gtgtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCaga                                                                                   >  1:3945039/1‑93 (MQ=255)
      gtgtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCaga                                                                                   >  1:4304018/1‑93 (MQ=255)
      gtgtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCaga                                                                                   <  1:2030258/93‑1 (MQ=255)
       ggtCGTACAGACGGAAAGCAGTTGCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCagag                                                                                  <  2:3435550/92‑1 (MQ=255)
        gtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGt                                                                                 <  2:1606470/93‑1 (MQ=255)
        gtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGt                                                                                 >  2:2309393/1‑93 (MQ=255)
         tCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCATCAACGCCAGACCGTGCTCCAGAGTa                                                                                <  2:3831553/93‑1 (MQ=255)
         tCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTa                                                                                <  2:3140162/93‑1 (MQ=255)
          cGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTAc                                                                               <  1:5042852/93‑1 (MQ=255)
           gTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACg                                                                              >  2:1956404/1‑93 (MQ=255)
           gTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGAGCTCCAGAGTACg                                                                              >  1:3043142/1‑93 (MQ=255)
            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             >  1:2207247/1‑93 (MQ=255)
            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             >  2:439139/1‑93 (MQ=255)
            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             <  2:1789055/93‑1 (MQ=255)
            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             <  2:4539538/93‑1 (MQ=255)
            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             >  1:6075055/1‑93 (MQ=255)
             aCAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAg                                                                            <  2:4611979/93‑1 (MQ=255)
             aCAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAg                                                                            >  1:516966/1‑93 (MQ=255)
             aCAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAg                                                                            <  1:2404394/93‑1 (MQ=255)
               aGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCa                                                                          >  1:2791955/1‑93 (MQ=255)
               aGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCa                                                                          >  1:1295303/1‑93 (MQ=255)
                  cGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAAc                                                                       <  1:779596/93‑1 (MQ=255)
                  cGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAAc                                                                       <  1:4147522/93‑1 (MQ=255)
                   ggAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACt                                                                      >  2:2868609/1‑93 (MQ=255)
                   ggAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACt                                                                      >  1:922684/1‑93 (MQ=255)
                   ggAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACt                                                                      <  1:3866490/93‑1 (MQ=255)
                     aaaGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGc                                                                    >  1:3195369/1‑93 (MQ=255)
                      aaGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGct                                                                   >  2:504939/1‑93 (MQ=255)
                       aGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGctc                                                                  <  1:1610470/93‑1 (MQ=255)
                        gCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGctct                                                                 >  2:4841456/1‑93 (MQ=255)
                        gCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGctct                                                                 <  2:5676370/93‑1 (MQ=255)
                             ttCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCttcttc                                                            <  2:812662/93‑1 (MQ=255)
                              tCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttca                                                           >  2:4643178/1‑93 (MQ=255)
                              tCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttca                                                           >  1:3317609/1‑93 (MQ=255)
                                aCCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagt                                                         >  2:2368616/1‑93 (MQ=255)
                                aCCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagt                                                         >  2:6161659/1‑93 (MQ=255)
                                 ccATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtc                                                        <  1:5437764/93‑1 (MQ=255)
                                 ccATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtc                                                        >  1:1048474/1‑93 (MQ=255)
                                  cATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtct                                                       <  2:3994578/93‑1 (MQ=255)
                                  cATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtct                                                       <  1:5856127/93‑1 (MQ=255)
                                  cATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtct                                                       >  2:3736543/1‑93 (MQ=255)
                                   aTCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtctt                                                      >  1:4696000/1‑93 (MQ=255)
                                    tCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtcttc                                                     <  1:6007292/93‑1 (MQ=255)
                                     ccAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtcttca                                                    <  1:1220006/93‑1 (MQ=255)
                                       aGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGc                                                  >  1:2269569/1‑93 (MQ=255)
                                        gCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCa                                                 <  2:5355610/93‑1 (MQ=255)
                                         cGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCAc                                                >  2:5761244/1‑93 (MQ=255)
                                          gTATCGCCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGTTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCAcc                                               <  1:3373925/93‑1 (MQ=255)
                                          gTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCAcc                                               <  1:3551839/93‑1 (MQ=255)
                                           tATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCt                                              >  1:5268292/1‑93 (MQ=255)
                                           tATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCt                                              >  1:4079779/1‑93 (MQ=255)
                                             tCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGc                                            >  1:6189962/1‑93 (MQ=255)
                                               aCCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTc                                          >  1:150427/1‑93 (MQ=255)
                                                ccAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCa                                         <  2:2178953/93‑1 (MQ=255)
                                                  aGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAc                                       >  2:3323287/1‑93 (MQ=255)
                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      <  2:1586742/93‑1 (MQ=255)
                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      <  1:1440440/93‑1 (MQ=255)
                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      >  2:1758882/1‑93 (MQ=255)
                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      <  1:3667740/93‑1 (MQ=255)
                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      >  1:3724259/1‑93 (MQ=255)
                                                     aaGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTg                                    <  2:2472591/93‑1 (MQ=255)
                                                      aGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTgc                                   <  2:1417712/93‑1 (MQ=255)
                                                       gTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTgcg                                  >  1:452347/1‑93 (MQ=255)
                                                         tttGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGcc                                <  2:1054075/93‑1 (MQ=255)
                                                          ttGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCct                               <  1:3110957/93‑1 (MQ=255)
                                                            gCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCctgc                             >  2:185375/1‑93 (MQ=255)
                                                             ccAGCTCTTCATCCAGCAACACCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCctgct                            >  2:6065193/1‑93 (MQ=255)
                                                                  tctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGt                       >  2:926708/1‑93 (MQ=255)
                                                                   ctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGtt                      >  1:5567900/1‑93 (MQ=255)
                                                                   ctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGtt                      >  1:5222455/1‑93 (MQ=255)
                                                                   ctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGtt                      <  1:3607919/93‑1 (MQ=255)
                                                                     tCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTc                    <  1:2090100/93‑1 (MQ=255)
                                                                     tCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTc                    <  1:2744105/93‑1 (MQ=255)
                                                                      cATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCa                   <  1:5316026/93‑1 (MQ=255)
                                                                       aTCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAg                  <  2:5971247/93‑1 (MQ=255)
                                                                       aTCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAg                  <  2:5161047/93‑1 (MQ=255)
                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAACCTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 <  2:6309000/93‑1 (MQ=255)
                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 >  2:6238461/1‑93 (MQ=255)
                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 <  2:1307828/93‑1 (MQ=255)
                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 <  1:2528812/93‑1 (MQ=255)
                                                                         ccAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGt                >  2:5208660/1‑93 (MQ=255)
                                                                          cAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTc               >  2:2838413/1‑93 (MQ=255)
                                                                            gCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCtt             >  2:3002700/1‑93 (MQ=255)
                                                                            gCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCtt             >  2:3335631/1‑93 (MQ=255)
                                                                             cAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTc            <  2:5270740/93‑1 (MQ=255)
                                                                             cAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTc            >  1:1100466/1‑93 (MQ=255)
                                                                              aaCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCGGGTCTTCa           <  1:1736252/93‑1 (MQ=255)
                                                                              aaCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCa           >  1:4924727/1‑93 (MQ=255)
                                                                              aaCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCa           >  1:1895987/1‑93 (MQ=255)
                                                                               aCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAc          >  1:4052589/1‑93 (MQ=255)
                                                                               aCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAc          >  2:1093551/1‑93 (MQ=255)
                                                                               aCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAc          >  2:3648333/1‑93 (MQ=255)
                                                                                 gCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAccc        <  1:4791253/93‑1 (MQ=255)
                                                                                  ccAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCg       <  1:2512577/93‑1 (MQ=255)
                                                                                   cAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGc      >  1:2666546/1‑93 (MQ=255)
                                                                                    aGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCa     <  2:1835811/93‑1 (MQ=255)
                                                                                     gACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCag    >  2:277595/1‑93 (MQ=255)
                                                                                       ccGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCagag  >  2:697811/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGCGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAG  >  NC_000913/2818826‑2819005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGCGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAGCCCA  >  NC_000913/2818823‑2819009
                                                                                             |                                                                                             
AAGCCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGT                                                                                               <  SRR4302533.1909986/94‑1 (MQ=60)
AAGCCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGT                                                                                               >  SRR4302533.585434/1‑94 (MQ=60)
AAGCCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGT                                                                                               >  SRR4302533.4235333/1‑94 (MQ=60)
    CATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTC                                                                                           <  SRR4302533.1935253/94‑1 (MQ=60)
    CATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTC                                                                                           >  SRR4302533.1188064/1‑94 (MQ=60)
     ATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCC                                                                                          <  SRR4302533.1087886/94‑1 (MQ=60)
     ATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCC                                                                                          <  SRR4302533.2259876/94‑1 (MQ=60)
     ATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCC                                                                                          >  SRR4302533.5672648/1‑94 (MQ=60)
     ATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCC                                                                                          >  SRR4302533.1508993/1‑94 (MQ=60)
       AGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAG                                                                                        <  SRR4302533.5913256/94‑1 (MQ=60)
        GGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGA                                                                                       >  SRR4302533.4329913/1‑94 (MQ=60)
        GGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGA                                                                                       >  SRR4302533.3969101/1‑94 (MQ=60)
         GTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAG                                                                                      >  SRR4302533.3955823/1‑94 (MQ=60)
         GTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAG                                                                                      <  SRR4302533.2042789/94‑1 (MQ=60)
          TGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGA                                                                                     >  SRR4302533.3071464/1‑94 (MQ=60)
           GTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTA                                                                                    <  SRR4302533.4814853/94‑1 (MQ=60)
             CGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACG                                                                                  <  SRR4302533.3375737/94‑1 (MQ=60)
             CGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACG                                                                                  <  SRR4302533.5073372/94‑1 (MQ=60)
             CGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACG                                                                                  <  SRR4302533.4066438/94‑1 (MQ=60)
             CGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGAGCTCCAGAGTACG                                                                                  >  SRR4302533.3061771/1‑94 (MQ=60)
              GTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGA                                                                                 >  SRR4302533.4033030/1‑94 (MQ=60)
              GTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGA                                                                                 >  SRR4302533.2220857/1‑94 (MQ=60)
              GTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGA                                                                                 >  SRR4302533.6111801/1‑94 (MQ=60)
               TACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAG                                                                                >  SRR4302533.520035/1‑94 (MQ=60)
                ACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGC                                                                               >  SRR4302533.2143753/1‑94 (MQ=60)
                ACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGC                                                                               <  SRR4302533.2419242/94‑1 (MQ=60)
                 CAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCA                                                                              <  SRR4302533.3555317/94‑1 (MQ=60)
                 CAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCA                                                                              <  SRR4302533.4160470/94‑1 (MQ=60)
                 CAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCA                                                                              >  SRR4302533.1303100/1‑94 (MQ=60)
                 CAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCA                                                                              <  SRR4302533.2919987/94‑1 (MQ=60)
                 CAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCA                                                                              >  SRR4302533.2809127/1‑94 (MQ=60)
                 CAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGCGCTCCAGAGTACGAGCA                                                                              <  SRR4302533.1109574/94‑1 (MQ=60)
                  AGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAA                                                                             >  SRR4302533.269295/1‑94 (MQ=60)
                    ACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAAC                                                                           >  SRR4302533.6113054/1‑94 (MQ=60)
                     CGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACT                                                                          >  SRR4302533.5954212/1‑94 (MQ=60)
                     CGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACT                                                                          <  SRR4302533.4172650/94‑1 (MQ=60)
                     CGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACT                                                                          >  SRR4302533.928105/1‑94 (MQ=60)
                     CGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACT                                                                          <  SRR4302533.784188/94‑1 (MQ=60)
                     CGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACT                                                                          >  SRR4302533.2311454/1‑94 (MQ=60)
                      GGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTG                                                                         <  SRR4302533.3890093/94‑1 (MQ=60)
                       GAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGC                                                                        >  SRR4302533.3215031/1‑94 (MQ=60)
                        AAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCT                                                                       <  SRR4302533.3888165/94‑1 (MQ=60)
                          AGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCT                                                                     >  SRR4302533.6064486/1‑94 (MQ=60)
                          AGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCT                                                                     <  SRR4302533.1620236/94‑1 (MQ=60)
                           GCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTT                                                                    >  SRR4302533.2539909/1‑94 (MQ=60)
                           GCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTT                                                                    <  SRR4302533.1248830/94‑1 (MQ=60)
                           GCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTT                                                                    <  SRR4302533.920481/94‑1 (MQ=60)
                            CAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTC                                                                   <  SRR4302533.1360940/94‑1 (MQ=60)
                             AGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCT                                                                  >  SRR4302533.1815491/1‑94 (MQ=60)
                             AGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCT                                                                  >  SRR4302533.1690416/1‑94 (MQ=60)
                              GTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTT                                                                 <  SRR4302533.5899295/94‑1 (MQ=60)
                              GTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTT                                                                 >  SRR4302533.3137303/1‑94 (MQ=60)
                                TTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCA                                                               >  SRR4302533.3338099/1‑94 (MQ=60)
                                 TCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAG                                                              <  SRR4302533.5314512/94‑1 (MQ=60)
                                  CACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGT                                                             >  SRR4302533.426605/1‑94 (MQ=60)
                                  CACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGT                                                             <  SRR4302533.5153120/94‑1 (MQ=60)
                                   ACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTC                                                            >  SRR4302533.1054636/1‑94 (MQ=60)
                                   ACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTC                                                            >  SRR4302533.2214766/1‑94 (MQ=60)
                                    CCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCT                                                           <  SRR4302533.2981443/94‑1 (MQ=60)
                                    CCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCT                                                           <  SRR4302533.5470517/94‑1 (MQ=60)
                                     CATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTT                                                          >  SRR4302533.4724464/1‑94 (MQ=60)
                                     CATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTT                                                          >  SRR4302533.2065906/1‑94 (MQ=60)
                                     CATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTT                                                          <  SRR4302533.5891529/94‑1 (MQ=60)
                                       TCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCA                                                        <  SRR4302533.6043686/94‑1 (MQ=60)
                                        CCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAG                                                       <  SRR4302533.1227329/94‑1 (MQ=60)
                                        CCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAG                                                       <  SRR4302533.3590601/94‑1 (MQ=60)
                                         CAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGC                                                      >  SRR4302533.2283610/1‑94 (MQ=60)
                                            CGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACC                                                   <  SRR4302533.1565102/94‑1 (MQ=60)
                                             GTATCGCCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGTTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCT                                                  <  SRR4302533.3394761/94‑1 (MQ=60)
                                             GTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCT                                                  <  SRR4302533.3573719/94‑1 (MQ=60)
                                             GTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCT                                                  <  SRR4302533.2638203/94‑1 (MQ=60)
                                             GTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCT                                                  >  SRR4302533.4104614/1‑94 (MQ=60)
                                             GTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCT                                                  >  SRR4302533.5299895/1‑94 (MQ=60)
                                             GTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCGCCAGAGGACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCT                                                  >  SRR4302533.2066690/1‑94 (MQ=60)
                                               ATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGC                                                >  SRR4302533.6227107/1‑94 (MQ=60)
                                                TCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCT                                               >  SRR4302533.3330171/1‑94 (MQ=60)
                                                TCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCT                                               <  SRR4302533.503185/94‑1 (MQ=60)
                                                TCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCT                                               <  SRR4302533.987006/94‑1 (MQ=60)
                                                 CACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTC                                              >  SRR4302533.4143471/1‑94 (MQ=60)
                                                 CACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTC                                              <  SRR4302533.5239802/94‑1 (MQ=60)
                                                 CACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTC                                              >  SRR4302533.3682838/1‑94 (MQ=60)
                                                 CACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTC                                              >  SRR4302533.151392/1‑94 (MQ=60)
                                                 CACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTC                                              >  SRR4302533.2409943/1‑94 (MQ=60)
                                                  ACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCA                                             >  SRR4302533.3157301/1‑94 (MQ=60)
                                                   CCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAA                                            <  SRR4302533.5282939/94‑1 (MQ=60)
                                                   CCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAA                                            <  SRR4302533.5282940/94‑1 (MQ=60)
                                                   CCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAA                                            >  SRR4302533.4479652/1‑94 (MQ=60)
                                                    CAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGATTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAC                                           <  SRR4302533.3394249/94‑1 (MQ=60)
                                                    CAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAC                                           <  SRR4302533.1409650/94‑1 (MQ=60)
                                                    CAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAC                                           >  SRR4302533.5306941/1‑94 (MQ=60)
                                                    CAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAC                                           >  SRR4302533.4729391/1‑94 (MQ=60)
                                                    CAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAC                                           <  SRR4302533.2258523/94‑1 (MQ=60)
                                                    CAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAC                                           >  SRR4302533.3066969/1‑94 (MQ=60)
                                                     AGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACC                                          >  SRR4302533.3747109/1‑94 (MQ=60)
                                                     AGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACC                                          >  SRR4302533.1254840/1‑94 (MQ=60)
                                                     AGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACC                                          <  SRR4302533.6231934/94‑1 (MQ=60)
                                                      GAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCT                                         >  SRR4302533.18942/1‑94 (MQ=60)
                                                      GAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCT                                         <  SRR4302533.5930054/94‑1 (MQ=60)
                                                      GAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCT                                         <  SRR4302533.1449130/94‑1 (MQ=60)
                                                      GAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCT                                         <  SRR4302533.3690284/94‑1 (MQ=60)
                                                       AAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTG                                        >  SRR4302533.5424498/1‑94 (MQ=60)
                                                         AGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCG                                      >  SRR4302533.455056/1‑94 (MQ=60)
                                                             TTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTG                                  <  SRR4302533.3130081/94‑1 (MQ=60)
                                                             TTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTG                                  <  SRR4302533.612832/94‑1 (MQ=60)
                                                              TGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGC                                 >  SRR4302533.35276/1‑94 (MQ=60)
                                                              TGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGC                                 >  SRR4302533.3726370/1‑94 (MQ=60)
                                                                 CAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGG                              <  SRR4302533.987801/94‑1 (MQ=60)
                                                                 CAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGG                              >  SRR4302533.5678821/1‑94 (MQ=60)
                                                                 CAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGG                              <  SRR4302533.2538101/94‑1 (MQ=60)
                                                                 CAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGG                              <  SRR4302533.2721118/94‑1 (MQ=60)
                                                                 CAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGG                              >  SRR4302533.4083997/1‑94 (MQ=60)
                                                                  CGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGC                             <  SRR4302533.1540326/94‑1 (MQ=60)
                                                                  AGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGC                             >  SRR4302533.5906777/1‑94 (MQ=60)
                                                                  AGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGC                             >  SRR4302533.2613592/1‑94 (MQ=60)
                                                                     TCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTT                          >  SRR4302533.5601555/1‑94 (MQ=60)
                                                                     TCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTT                          <  SRR4302533.90016/94‑1 (MQ=60)
                                                                     TCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTT                          <  SRR4302533.740207/94‑1 (MQ=60)
                                                                     TCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTT                          >  SRR4302533.5253877/1‑94 (MQ=60)
                                                                      CTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTT                         >  SRR4302533.3801402/1‑94 (MQ=60)
                                                                      CTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTT                         <  SRR4302533.3630130/94‑1 (MQ=60)
                                                                        TCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCA                       <  SRR4302533.2760928/94‑1 (MQ=60)
                                                                        TCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCA                       <  SRR4302533.2102971/94‑1 (MQ=60)
                                                                         CATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAG                      <  SRR4302533.5347881/94‑1 (MQ=60)
                                                                         CACCCCGCAACGCCAGACCGTGCTCCAGCGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAG                      <  SRR4302533.2356915/94‑1 (MQ=60)
                                                                          GTCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGG                     >  SRR4302533.55198/1‑94 (MQ=60)
                                                                          ATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGG                     >  SRR4302533.492507/1‑94 (MQ=60)
                                                                          ATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGG                     >  SRR4302533.4240389/1‑94 (MQ=60)
                                                                           TCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGT                    <  SRR4302533.2544381/94‑1 (MQ=60)
                                                                             CAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCT                  >  SRR4302533.4514837/1‑94 (MQ=60)
                                                                               GCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTC                >  SRR4302533.1106991/1‑94 (MQ=60)
                                                                                CAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCA               >  SRR4302533.1907558/1‑94 (MQ=60)
                                                                                CAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCA               >  SRR4302533.4954558/1‑94 (MQ=60)
                                                                                CAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCA               >  SRR4302533.5354860/1‑94 (MQ=60)
                                                                                CAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCA               <  SRR4302533.2455395/94‑1 (MQ=60)
                                                                                 AACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCGGGTCTTCAC              <  SRR4302533.1746788/94‑1 (MQ=60)
                                                                                 AACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAC              <  SRR4302533.2795847/94‑1 (MQ=60)
                                                                                 AACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAC              >  SRR4302533.4077268/1‑94 (MQ=60)
                                                                                 AACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAC              >  SRR4302533.4931981/1‑94 (MQ=60)
                                                                                 AACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAC              >  SRR4302533.5102339/1‑94 (MQ=60)
                                                                                   CGCGAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCC            <  SRR4302533.170863/94‑1 (MQ=60)
                                                                                    GCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCG           <  SRR4302533.4820285/94‑1 (MQ=60)
                                                                                     CCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGC          >  SRR4302533.2682908/1‑94 (MQ=60)
                                                                                     CCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGC          >  SRR4302533.3901395/1‑94 (MQ=60)
                                                                                     CCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGC          <  SRR4302533.2528058/94‑1 (MQ=60)
                                                                                       AGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAG        <  SRR4302533.470959/94‑1 (MQ=60)
                                                                                       AGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAG        >  SRR4302533.3681088/1‑94 (MQ=60)
                                                                                        GACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGA       >  SRR4302533.3581940/1‑94 (MQ=60)
                                                                                         ACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAG      <  SRR4302533.5015508/94‑1 (MQ=60)
                                                                                          CCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAGC     >  SRR4302533.3642281/1‑94 (MQ=60)
                                                                                          CCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAGC     >  SRR4302533.4816654/1‑94 (MQ=60)
                                                                                          CCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAGC     >  SRR4302533.1778363/1‑94 (MQ=60)
                                                                                            GTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAGCCC   >  SRR4302533.5679392/1‑94 (MQ=60)
                                                                                             TGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAGCCCA  >  SRR4302533.1816788/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAGCCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGCGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAGCCCA  >  NC_000913/2818823‑2819009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑