Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,868,544	T→C	S262G (AGT→GGT)	ygbO ←	putative hydrogenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,868,544	0	T	C	100.0%	715.0 / NA	221	S262G (AGT→GGT)	ygbO	putative hydrogenase subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (99/122); total (99/122)

Only 100 of 224 total aligned reads displayed.

GCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGC  >  NC_000913/2868455‑2868633

gcgcACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGcc                                                                                        <  2:2830352/93‑1 (MQ=255)

  gcACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTg                                                                                      <  2:2940698/93‑1 (MQ=255)

  gcACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTg                                                                                      <  2:6268953/93‑1 (MQ=255)

    aCCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGc                                                                                    <  2:5779278/93‑1 (MQ=255)

    aCCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGACAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGc                                                                                    >  2:1376489/1‑93 (MQ=255)

      cAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCaa                                                                                  <  2:1717799/93‑1 (MQ=255)

       aGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAAt                                                                                 <  1:2386250/93‑1 (MQ=255)

        gTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATg                                                                                <  1:371333/93‑1 (MQ=255)

        gTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATg                                                                                <  2:1829776/93‑1 (MQ=255)

         tAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATgc                                                                               >  1:2859624/1‑93 (MQ=255)

          aaaGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATgcg                                                                              <  2:1141533/93‑1 (MQ=255)

          aaaGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATgcg                                                                              >  2:5928074/1‑93 (MQ=255)

            aGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATgcggc                                                                            <  2:4534744/93‑1 (MQ=255)

            aGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATgcggc                                                                            <  1:811427/93‑1 (MQ=255)

             gCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATgcggcg                                                                           <  1:296904/93‑1 (MQ=255)

             gCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATgcggcg                                                                           >  1:1758307/1‑93 (MQ=255)

              cTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGcggcgg                                                                          <  2:3944610/93‑1 (MQ=255)

              cTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGcggcgg                                                                          <  1:4337316/93‑1 (MQ=255)

              cTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGcggcgg                                                                          >  2:4912402/1‑93 (MQ=255)

               ttGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGt                                                                         <  2:1243321/93‑1 (MQ=255)

                 gTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTa                                                                       <  1:4620475/93‑1 (MQ=255)

                 gTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTa                                                                       <  1:5100345/93‑1 (MQ=255)

                 gTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGCTa                                                                       >  2:610621/1‑93 (MQ=255)

                  tAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCCCCGCCTGGCAATGCGGCGGTTat                                                                      <  2:5872666/93‑1 (MQ=255)

                     ttCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTatcat                                                                   <  1:3109636/93‑1 (MQ=255)

                     ttCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTatcat                                                                   >  1:4226103/1‑93 (MQ=255)

                      tCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTatcatc                                                                  >  1:229105/1‑93 (MQ=255)

                      tCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTatcatc                                                                  <  2:2671528/93‑1 (MQ=255)

                      tCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTatcatc                                                                  >  1:5122584/1‑93 (MQ=255)

                       cAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTAtcatca                                                                 <  2:4317032/93‑1 (MQ=255)

                       cAGTTTCTGCGGCGACAGCTGCTTGCTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTatcatta                                                                 <  1:6089380/93‑3 (MQ=255)

                        aGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCaa                                                                >  2:945831/1‑93 (MQ=255)

                        aGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCaa                                                                >  1:244081/1‑93 (MQ=255)

                          tttCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAAct                                                              <  2:1426328/93‑1 (MQ=255)

                            tCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAActct                                                            <  1:4287469/93‑1 (MQ=255)

                            tCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAActct                                                            >  1:3998145/1‑93 (MQ=255)

                            tCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAActct                                                            >  2:1955316/1‑93 (MQ=255)

                             cTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCtt                                                           >  2:3324723/1‑93 (MQ=255)

                               gcggcgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTa                                                         >  1:4809346/1‑93 (MQ=255)

                               gcggcgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTa                                                         <  1:543159/93‑1 (MQ=255)

                               gcggcgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTa                                                         <  1:208046/93‑1 (MQ=255)

                               gcggcgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTa                                                         <  1:2608895/93‑1 (MQ=255)

                               gcggcgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTa                                                         <  2:2035007/93‑1 (MQ=255)

                                 ggcgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATc                                                       <  1:5508537/93‑1 (MQ=255)

                                  gcgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCg                                                      <  2:2360555/93‑1 (MQ=255)

                                   cgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGt                                                     >  1:4161679/1‑93 (MQ=255)

                                   cgACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGt                                                     <  1:1016543/93‑1 (MQ=255)

                                    gACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGtt                                                    >  1:1630801/1‑93 (MQ=255)

                                    gACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGtt                                                    >  2:3115876/1‑93 (MQ=255)

                                     aCAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTg                                                   >  2:552411/1‑93 (MQ=255)

                                     aCAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTg                                                   >  2:5335091/1‑93 (MQ=255)

                                      cAGCTGCTTGTTCGAATGCCAGCTCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTga                                                  <  1:3494236/93‑1 (MQ=255)

                                      cAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTga                                                  <  2:2251701/93‑1 (MQ=255)

                                      cAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTga                                                  <  2:5088959/93‑1 (MQ=255)

                                      cAGCTGCTTGTTCGAATGCCAGCGCTCCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTga                                                  <  2:3760003/93‑1 (MQ=255)

                                       aGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCACCTCTTTATCGTTgac                                                 <  2:3456226/93‑1 (MQ=255)

                                       aGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgac                                                 >  1:1100583/1‑93 (MQ=255)

                                       aGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgac                                                 <  2:795958/93‑1 (MQ=255)

                                       aGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgac                                                 <  2:4638345/93‑1 (MQ=255)

                                       aGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgac                                                 >  1:5725710/1‑93 (MQ=255)

                                          tgctTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgcg                                              >  2:5707149/1‑93 (MQ=255)

                                          tgctTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgcg                                              >  1:4902667/1‑93 (MQ=255)

                                           gctTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgcga                                             <  2:3135674/93‑1 (MQ=255)

                                             ttGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgcgacg                                           >  1:5821364/1‑93 (MQ=255)

                                               gTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCt                                         >  2:4727942/1‑93 (MQ=255)

                                                 tCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGt                                       <  1:3559726/93‑1 (MQ=255)

                                                  cGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTa                                      <  1:5672522/93‑1 (MQ=255)

                                                    aaTGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAAt                                    >  1:930217/1‑93 (MQ=255)

                                                    aaTGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAAt                                    <  1:6201095/93‑1 (MQ=255)

                                                     aTGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAAtt                                   >  1:6259700/1‑93 (MQ=255)

                                                      tGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTc                                  >  2:2183810/1‑93 (MQ=255)

                                                      tGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTc                                  <  2:5629730/93‑1 (MQ=255)

                                                        ccAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCg                                >  2:5278095/1‑93 (MQ=255)

                                                        ccAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCg                                >  1:4412074/1‑93 (MQ=255)

                                                         cAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGc                               >  2:752471/1‑93 (MQ=255)

                                                          aGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGcc                              <  2:820197/93‑1 (MQ=255)

                                                          aGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGcc                              >  1:885289/1‑93 (MQ=255)

                                                          aGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGcc                              >  1:2718762/1‑93 (MQ=255)

                                                           gcgcTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCa                             <  1:5520758/93‑1 (MQ=255)

                                                                tCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGttct                        <  2:4195895/93‑1 (MQ=255)

                                                                tCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGttct                        >  1:1505290/1‑93 (MQ=255)

                                                                 cACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGttctt                       <  1:2174641/93‑1 (MQ=255)

                                                                 cACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGttctt                       <  1:5384816/93‑1 (MQ=255)

                                                                    gCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCgg                    >  1:2671017/1‑93 (MQ=255)

                                                                     cTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCggt                   >  1:723214/1‑93 (MQ=255)

                                                                     cTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCggt                   <  1:366940/93‑1 (MQ=255)

                                                                     cTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCggt                   >  1:1912407/1‑93 (MQ=255)

                                                                         gTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGtt               <  1:5064776/93‑1 (MQ=255)

                                                                              ccATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGAc          >  1:4476427/1‑93 (MQ=255)

                                                                                aTTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACaa        <  1:5748250/93‑1 (MQ=255)

                                                                                aTTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACaa        >  2:1296007/1‑93 (MQ=255)

                                                                                 ttCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACaaa       <  1:2528236/93‑1 (MQ=255)

                                                                                  tCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAAc      <  1:3774234/93‑1 (MQ=255)

                                                                                  tCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAAc      >  1:4377025/1‑93 (MQ=255)

                                                                                  tCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAAc      <  1:1998528/93‑1 (MQ=255)

                                                                                  tCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAAc      <  2:4136348/93‑1 (MQ=255)

                                                                                   cgccgccgcCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAAcc     <  1:6038342/93‑1 (MQ=255)

                                                                                    gCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCa    >  2:2432185/1‑93 (MQ=255)

                                                                                     ccACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAg   >  2:2989783/1‑93 (MQ=255)

                                                                                      cACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGc  <  2:5256197/93‑1 (MQ=255)

GCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGC  >  NC_000913/2868455‑2868633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTCGCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTACC  >  NC_000913/2868451‑2868637

TTTCGCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACC                                                                                               <  SRR4302533.1049975/94‑1 (MQ=60)

TTTCGCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACC                                                                                               <  SRR4302533.5294290/94‑1 (MQ=60)

    GCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCT                                                                                           >  SRR4302533.729322/1‑94 (MQ=60)

     CGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTG                                                                                          >  SRR4302533.5814312/1‑94 (MQ=60)

     CGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTG                                                                                          <  SRR4302533.545223/94‑1 (MQ=60)

       CACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGC                                                                                        >  SRR4302533.477313/1‑94 (MQ=60)

        ACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCA                                                                                       >  SRR4302533.3723991/1‑94 (MQ=60)

        ACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCA                                                                                       >  SRR4302533.5734298/1‑94 (MQ=60)

         CCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAA                                                                                      <  SRR4302533.4135674/94‑1 (MQ=60)

           AGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATG                                                                                    <  SRR4302533.2400996/94‑1 (MQ=60)

           AGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATG                                                                                    <  SRR4302533.6340528/94‑1 (MQ=60)

            GTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGC                                                                                   <  SRR4302533.373574/94‑1 (MQ=60)

            GTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGC                                                                                   >  SRR4302533.2877217/1‑94 (MQ=60)

             TAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCG                                                                                  <  SRR4302533.880020/94‑1 (MQ=60)

              AAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGG                                                                                 >  SRR4302533.2401907/1‑94 (MQ=60)

               AAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGC                                                                                <  SRR4302533.1527857/94‑1 (MQ=60)

                AGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCG                                                                               >  SRR4302533.1769025/1‑94 (MQ=60)

                AGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCG                                                                               <  SRR4302533.816182/94‑1 (MQ=60)

                 GCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGG                                                                              <  SRR4302533.298722/94‑1 (MQ=60)

                  CTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGT                                                                             <  SRR4302533.4363457/94‑1 (MQ=60)

                  CTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGT                                                                             <  SRR4302533.4329433/94‑1 (MQ=60)

                  CTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGT                                                                             >  SRR4302533.661043/1‑94 (MQ=60)

                   TTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTT                                                                            >  SRR4302533.4370979/1‑94 (MQ=60)

                    TGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTA                                                                           <  SRR4302533.2662292/94‑1 (MQ=60)

                     GTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTAT                                                                          <  SRR4302533.4648478/94‑1 (MQ=60)

                     GTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTAT                                                                          <  SRR4302533.5131155/94‑1 (MQ=60)

                       AATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCA                                                                        >  SRR4302533.5171091/1‑94 (MQ=60)

                        ATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCAT                                                                       <  SRR4302533.5096138/94‑1 (MQ=60)

                        ATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCAT                                                                       >  SRR4302533.4251661/1‑94 (MQ=60)

                         TTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATC                                                                      <  SRR4302533.3128749/94‑1 (MQ=60)

                         TTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATC                                                                      >  SRR4302533.230526/1‑94 (MQ=60)

                         TTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATC                                                                      >  SRR4302533.5153535/1‑94 (MQ=60)

                         TTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATC                                                                      >  SRR4302533.618247/1‑94 (MQ=60)

                         TTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATC                                                                      <  SRR4302533.5002315/94‑1 (MQ=60)

                          TCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCA                                                                     >  SRR4302533.4910139/1‑94 (MQ=60)

                           CAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAA                                                                    >  SRR4302533.245594/1‑94 (MQ=60)

                           CAGTTTCTGCGGCGACAGCTGCTTGCTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATTAA                                                                    <  SRR4302533.6126189/94‑1 (MQ=60)

                            AGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAAC                                                                   <  SRR4302533.2871056/94‑1 (MQ=60)

                            AGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAAC                                                                   >  SRR4302533.569850/1‑94 (MQ=60)

                             GTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACT                                                                  <  SRR4302533.3880780/94‑1 (MQ=60)

                             GTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACT                                                                  <  SRR4302533.31029/94‑1 (MQ=60)

                              TTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTC                                                                 <  SRR4302533.5557246/94‑1 (MQ=60)

                               TTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCT                                                                >  SRR4302533.2094143/1‑94 (MQ=60)

                               TTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCT                                                                >  SRR4302533.4022487/1‑94 (MQ=60)

                               TTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCT                                                                <  SRR4302533.4427617/94‑1 (MQ=60)

                                TCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTT                                                               <  SRR4302533.4313250/94‑1 (MQ=60)

                                 CTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTT                                                              <  SRR4302533.5491067/94‑1 (MQ=60)

                                  TGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTA                                                             >  SRR4302533.4838495/1‑94 (MQ=60)

                                   GCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTAT                                                            <  SRR4302533.209363/94‑1 (MQ=60)

                                   GCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTAT                                                            <  SRR4302533.2463320/94‑1 (MQ=60)

                                   GCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTAT                                                            <  SRR4302533.546382/94‑1 (MQ=60)

                                   GCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTAT                                                            <  SRR4302533.2624914/94‑1 (MQ=60)

                                    CGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATC                                                           <  SRR4302533.5764103/94‑1 (MQ=60)

                                     GGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCG                                                          >  SRR4302533.3675787/1‑94 (MQ=60)

                                     GGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCG                                                          <  SRR4302533.5541789/94‑1 (MQ=60)

                                      GCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGT                                                         <  SRR4302533.3540629/94‑1 (MQ=60)

                                      GCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGT                                                         >  SRR4302533.4186897/1‑94 (MQ=60)

                                      GCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCCTTATCGT                                                         <  SRR4302533.405330/94‑1 (MQ=60)

                                       CGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTT                                                        <  SRR4302533.1022517/94‑1 (MQ=60)

                                       CGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTT                                                        <  SRR4302533.186684/94‑1 (MQ=60)

                                       CGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTT                                                        <  SRR4302533.2932741/94‑1 (MQ=60)

                                       CGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTT                                                        <  SRR4302533.1798250/94‑1 (MQ=60)

                                       CGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTT                                                        >  SRR4302533.5136797/1‑94 (MQ=60)

                                       CGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTT                                                        >  SRR4302533.1640678/1‑94 (MQ=60)

                                       CGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTT                                                        >  SRR4302533.930539/1‑94 (MQ=60)

                                          CAGCTGCTTGTTCGAATGCCAGCTCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGAC                                                     <  SRR4302533.3515789/94‑1 (MQ=60)

                                          CAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGAC                                                     >  SRR4302533.5760278/1‑94 (MQ=60)

                                          CAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGAC                                                     >  SRR4302533.1107108/1‑94 (MQ=60)

                                          CAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGAC                                                     <  SRR4302533.3768506/94‑1 (MQ=60)

                                           AGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACG                                                    >  SRR4302533.2084818/1‑94 (MQ=60)

                                             CTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCG                                                  >  SRR4302533.4932373/1‑94 (MQ=60)

                                               GCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGAC                                                <  SRR4302533.1763223/94‑1 (MQ=60)

                                               GCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGCTATCATCAACTCTTTATCGTTGACGCGAC                                                >  SRR4302533.5955190/1‑94 (MQ=60)

                                                CTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACG                                               >  SRR4302533.5856527/1‑94 (MQ=60)

                                                CTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACG                                               >  SRR4302533.3152767/1‑94 (MQ=60)

                                                     TCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTA                                          <  SRR4302533.3581659/94‑1 (MQ=60)

                                                      CGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAA                                         >  SRR4302533.2381517/1‑94 (MQ=60)

                                                      CGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAA                                         <  SRR4302533.5706782/94‑1 (MQ=60)

                                                       GAATGCGAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAAT                                        <  SRR4302533.2718154/94‑1 (MQ=60)

                                                       GAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAAT                                        >  SRR4302533.935678/1‑94 (MQ=60)

                                                       GAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAAT                                        <  SRR4302533.4577521/94‑1 (MQ=60)

                                                        AATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATT                                       >  SRR4302533.6297127/1‑94 (MQ=60)

                                                        AATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATT                                       <  SRR4302533.6238288/94‑1 (MQ=60)

                                                        AATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATT                                       <  SRR4302533.2953905/94‑1 (MQ=60)

                                                         ATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTC                                      >  SRR4302533.1971887/1‑94 (MQ=60)

                                                          TGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCC                                     <  SRR4302533.6293974/94‑1 (MQ=60)

                                                           GCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCG                                    >  SRR4302533.4438718/1‑94 (MQ=60)

                                                            CCAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGC                                   >  SRR4302533.4234023/1‑94 (MQ=60)

                                                             CAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCC                                  >  SRR4302533.2735450/1‑94 (MQ=60)

                                                             CAGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCC                                  >  SRR4302533.890491/1‑94 (MQ=60)

                                                              AGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCA                                 >  SRR4302533.4866895/1‑94 (MQ=60)

                                                              AGCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCA                                 >  SRR4302533.1283632/1‑94 (MQ=60)

                                                               GCGCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAG                                <  SRR4302533.5554089/94‑1 (MQ=60)

                                                                 GCTTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTT                              <  SRR4302533.4295860/94‑1 (MQ=60)

                                                                   TTCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCT                            >  SRR4302533.1514370/1‑94 (MQ=60)

                                                                    TCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTT                           <  SRR4302533.2598582/94‑1 (MQ=60)

                                                                    TCACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTT                           <  SRR4302533.5542233/94‑1 (MQ=60)

                                                                     CACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTC                          <  SRR4302533.5417168/94‑1 (MQ=60)

                                                                     CACGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTC                          <  SRR4302533.2188042/94‑1 (MQ=60)

                                                                       CGCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGG                        >  SRR4302533.2687400/1‑94 (MQ=60)

                                                                        GCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGT                       >  SRR4302533.2222514/1‑94 (MQ=60)

                                                                        GCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGT                       >  SRR4302533.727490/1‑94 (MQ=60)

                                                                        GCTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGT                       >  SRR4302533.1924074/1‑94 (MQ=60)

                                                                         CTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTG                      <  SRR4302533.3677850/94‑1 (MQ=60)

                                                                         CTGAGTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTG                      <  SRR4302533.369159/94‑1 (MQ=60)

                                                                             GTTCCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTG                  <  SRR4302533.5095370/94‑1 (MQ=60)

                                                                                CCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGA               >  SRR4302533.448654/1‑94 (MQ=60)

                                                                                CCCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGA               <  SRR4302533.22256/94‑1 (MQ=60)

                                                                                 CCCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGAC              >  SRR4302533.4503501/1‑94 (MQ=60)

                                                                                  CCATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACA             >  SRR4302533.6089566/1‑94 (MQ=60)

                                                                                   CATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAA            >  SRR4302533.6049684/1‑94 (MQ=60)

                                                                                    ATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAA           <  SRR4302533.5782928/94‑1 (MQ=60)

                                                                                    ATTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAA           <  SRR4302533.5196197/94‑1 (MQ=60)

                                                                                     TTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAAC          <  SRR4302533.2543802/94‑1 (MQ=60)

                                                                                     TTCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAAC          >  SRR4302533.4403449/1‑94 (MQ=60)

                                                                                      TCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACC         <  SRR4302533.2010825/94‑1 (MQ=60)

                                                                                      TCGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACC         <  SRR4302533.3797370/94‑1 (MQ=60)

                                                                                       CGCCGCCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCA        <  SRR4302533.6074934/94‑1 (MQ=60)

                                                                                       CGCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCA        <  SRR4302533.4436879/94‑1 (MQ=60)

                                                                                        GCGACCGCCTTGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAG       <  SRR4302533.3017915/94‑1 (MQ=60)

                                                                                        GCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAG       <  SRR4302533.1040136/94‑1 (MQ=60)

                                                                                        GCCACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAG       <  SRR4302533.2590650/94‑1 (MQ=60)

                                                                                          CACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCT     >  SRR4302533.762636/1‑94 (MQ=60)

                                                                                          CACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCT     >  SRR4302533.1240954/1‑94 (MQ=60)

                                                                                           ACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTA    <  SRR4302533.4340253/94‑1 (MQ=60)

                                                                                           ACCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTA    >  SRR4302533.4812096/1‑94 (MQ=60)

                                                                                            CCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTAC   <  SRR4302533.6002900/94‑1 (MQ=60)

                                                                                            CCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTAC   <  SRR4302533.6002899/94‑1 (MQ=60)

                                                                                            CCGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTAC   <  SRR4302533.1450172/94‑1 (MQ=60)

                                                                                             CGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTACC  <  SRR4302533.3026542/94‑1 (MQ=60)

TTTCGCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTCGGTGGTTGCGACAAACCAGCTACC  >  NC_000913/2868451‑2868637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`