Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,954,264	A→T	D881E (GAT→GAA)	ptr ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,954,264	0	A	T	99.6%	898.6 / NA	244	D881E (GAT→GAA)	ptr	protease III
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (125/118); minor base G (1/0); total (126/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGATCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCG  >  NC_000913/2954174‑2954352

cTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTc                                                                                        >  1:1696001/1‑93 (MQ=255)

cTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTc                                                                                        <  1:5968282/93‑1 (MQ=255)

 tGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCg                                                                                       >  2:681716/1‑93 (MQ=255)

    tGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGaaa                                                                                    <  1:4478498/93‑1 (MQ=255)

     ggAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAAt                                                                                   >  1:3119058/1‑93 (MQ=255)

     ggAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAAt                                                                                   <  1:800181/93‑1 (MQ=255)

       aagaaATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCt                                                                                 <  2:1906013/93‑1 (MQ=255)

        agaaATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCtt                                                                                <  2:6020988/93‑1 (MQ=255)

         gaaATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCttt                                                                               >  1:4652903/1‑93 (MQ=255)

         gaaATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCttt                                                                               >  1:2302732/1‑93 (MQ=255)

         gaaATCAGCAAGTTTTTGCGGCGTCAGCAGGTTTATCTGGGCCACGATTTTATCACGCGCATCGAAGCGCATATTGCCGCGTTCGCAATCttt                                                                               >  1:5395612/1‑93 (MQ=255)

           aaTCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTAc                                                                             <  2:4261994/93‑1 (MQ=255)

           aaTCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTAc                                                                             <  2:6294507/93‑1 (MQ=255)

            aTCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACt                                                                            <  2:2651335/93‑1 (MQ=255)

              cAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTa                                                                          >  2:116220/1‑93 (MQ=255)

               aGCAAGTTTTTGCGGCGTCAGCAGGTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTaa                                                                         >  2:3564354/1‑93 (MQ=255)

                gCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTagc                                                                        <  2:4321087/93‑3 (MQ=255)

                gCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAAc                                                                        <  2:3675961/93‑1 (MQ=255)

                  aaGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACtt                                                                      >  1:4999705/1‑93 (MQ=255)

                  aaGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACtt                                                                      >  2:399771/1‑93 (MQ=255)

                  aaGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACtt                                                                      <  1:5140785/93‑1 (MQ=255)

                   aGTTTTTGCGTCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTc                                                                     <  2:2527959/93‑1 (MQ=255)

                   aGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTc                                                                     <  2:4968616/93‑1 (MQ=255)

                   aGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTc                                                                     >  2:1252172/1‑93 (MQ=255)

                   aGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTc                                                                     <  2:1507635/93‑1 (MQ=255)

                     tttttGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGa                                                                   <  2:5154970/93‑1 (MQ=255)

                     tttttGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGa                                                                   >  1:4298518/1‑93 (MQ=255)

                     tttttGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGa                                                                   <  2:909367/93‑1 (MQ=255)

                      ttttGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGAt                                                                  <  2:3092516/93‑1 (MQ=255)

                       tttGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATg                                                                 <  2:1738768/93‑1 (MQ=255)

                          gcggcgTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGctt                                                              >  2:3338504/1‑93 (MQ=255)

                            ggcgTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGcttct                                                            >  1:3418512/1‑93 (MQ=255)

                            ggcgTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGcttct                                                            >  1:2512290/1‑93 (MQ=255)

                            ggcgTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGcttct                                                            >  2:6111346/1‑93 (MQ=255)

                             gcgTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGcttctt                                                           <  1:5184864/93‑1 (MQ=255)

                               gTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCg                                                         <  2:4657706/93‑1 (MQ=255)

                               gTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCg                                                         >  2:2220803/1‑93 (MQ=255)

                                tCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGc                                                        >  1:1098178/1‑93 (MQ=255)

                                  agcagTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCg                                                      <  2:3757183/93‑1 (MQ=255)

                                  agcagTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCg                                                      <  2:5830866/93‑1 (MQ=255)

                                   gcagTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGa                                                     >  2:1604086/1‑93 (MQ=255)

                                   gcagTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGa                                                     <  2:403774/93‑1 (MQ=255)

                                    cagTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAg                                                    <  2:4843344/93‑1 (MQ=255)

                                     agTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGc                                                   >  1:3575101/1‑93 (MQ=255)

                                     agTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGc                                                   <  1:6240489/93‑1 (MQ=255)

                                     agTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGc                                                   >  1:488545/1‑93 (MQ=255)

                                      gTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCg                                                  >  2:6215186/1‑93 (MQ=255)

                                       ttttATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGt                                                 >  2:3217999/1‑93 (MQ=255)

                                       ttttATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGt                                                 >  2:2899179/1‑93 (MQ=255)

                                       ttttATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGt                                                 >  1:3968942/1‑93 (MQ=255)

                                       ttttATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGt                                                 >  1:1894337/1‑93 (MQ=255)

                                        tttATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGtt                                                <  2:5345588/93‑1 (MQ=255)

                                          tATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTg                                              <  1:5464797/93‑1 (MQ=255)

                                           aTCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGc                                             <  1:1413256/93‑1 (MQ=255)

                                             cTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCgg                                           <  2:595069/93‑1 (MQ=255)

                                             cTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCgg                                           <  1:1273565/93‑1 (MQ=255)

                                             cTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCgg                                           <  1:2413490/93‑1 (MQ=255)

                                             cTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCgg                                           <  1:5247160/93‑1 (MQ=255)

                                               gggCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTg                                         >  1:5591998/1‑93 (MQ=255)

                                                 gCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGcc                                       <  1:3759407/93‑1 (MQ=255)

                                                 gCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGcc                                       >  1:4923600/1‑93 (MQ=255)

                                                  ccACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCt                                      >  1:4100178/1‑93 (MQ=255)

                                                   cACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTg                                     <  2:4545267/93‑1 (MQ=255)

                                                    aCGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTgc                                    >  2:5550777/1‑93 (MQ=255)

                                                     cGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTgca                                   <  1:5647075/93‑1 (MQ=255)

                                                       aTTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTgcagc                                 >  1:5026204/1‑93 (MQ=255)

                                                        ttttATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTgcagca                                <  2:3827020/93‑1 (MQ=255)

                                                        ttttATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTgcagca                                <  1:2696182/93‑1 (MQ=255)

                                                         tttATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCAt                               >  2:6050853/1‑93 (MQ=255)

                                                          ttATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATc                              <  1:21613/93‑1 (MQ=255)

                                                           tATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCt                             >  2:2079754/1‑93 (MQ=255)

                                                            accACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTg                            <  2:3283148/91‑1 (MQ=255)

                                                             tCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTgg                           >  1:6070760/1‑93 (MQ=255)

                                                              cACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTggg                          <  2:2260025/93‑1 (MQ=255)

                                                              cACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTggg                          <  2:562363/93‑1 (MQ=255)

                                                               aCGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGt                         >  2:2143403/1‑93 (MQ=255)

                                                               aCGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGt                         >  1:1568042/1‑93 (MQ=255)

                                                                cgcgAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTa                        <  1:2864837/93‑1 (MQ=255)

                                                                   gAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAAtt                     >  1:2664363/1‑93 (MQ=255)

                                                                   gAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAAtt                     <  1:720730/93‑1 (MQ=255)

                                                                    aaTCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTa                    >  2:52517/1‑93 (MQ=255)

                                                                      tCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTAcc                  <  2:4098044/93‑1 (MQ=255)

                                                                      tCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGGTTGCGGTGCCTGCAGCATCTGGGTAATTAcc                  >  1:3231850/1‑93 (MQ=255)

                                                                       cGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCg                 <  1:4189667/93‑1 (MQ=255)

                                                                        gAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGc                >  1:4363350/1‑93 (MQ=255)

                                                                         aaGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGcc               <  2:112932/93‑1 (MQ=255)

                                                                         aaGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGcc               >  1:6198398/1‑93 (MQ=255)

                                                                           gcgcATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCctg             <  2:3273416/93‑1 (MQ=255)

                                                                            cgcATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCctgc            <  2:814452/93‑1 (MQ=255)

                                                                              gatatTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCGGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCctgctg          <  2:3203665/92‑1 (MQ=255)

                                                                              cATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCctgctg          >  1:4657664/1‑93 (MQ=255)

                                                                               atatTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTgg         >  1:3392714/1‑93 (MQ=255)

                                                                                tatTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGa        >  1:3179365/1‑93 (MQ=255)

                                                                                tatTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGa        <  1:5731103/93‑1 (MQ=255)

                                                                                tatTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGa        >  1:198124/1‑93 (MQ=255)

                                                                                  ttGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGTAGCATCTGGGTAATTACCGCCTGCTGGAtt      >  2:1373158/1‑93 (MQ=255)

                                                                                   tGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGAttt     <  1:1376667/93‑1 (MQ=255)

                                                                                   tGCCGCGGTCGAAATCTTTTTTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGAttt     <  2:4560191/93‑1 (MQ=255)

                                                                                    gCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTg    <  1:3501702/93‑1 (MQ=255)

                                                                                     ccGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGGAATTACCGCCTGCTGGATTTgc   <  1:4130930/93‑1 (MQ=255)

CTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGATCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCG  >  NC_000913/2954174‑2954352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGATCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCGCAAAC  >  NC_000913/2954171‑2954357

CGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGT                                                                                               <  SRR4302533.1191765/94‑1 (MQ=60)

 GCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTT                                                                                              <  SRR4302533.4284955/94‑1 (MQ=60)

  CCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTC                                                                                             <  SRR4302533.1117681/94‑1 (MQ=60)

  CCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTC                                                                                             >  SRR4302533.1706273/1‑94 (MQ=60)

  CCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTC                                                                                             >  SRR4302533.1342990/1‑94 (MQ=60)

  CCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTC                                                                                             >  SRR4302533.1628977/1‑94 (MQ=60)

   CTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGCTTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCG                                                                                            >  SRR4302533.4991563/1‑94 (MQ=60)

   CTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCG                                                                                            <  SRR4302533.2615847/94‑1 (MQ=60)

   CTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCG                                                                                            >  SRR4302533.478534/1‑94 (MQ=60)

   CTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCG                                                                                            <  SRR4302533.6004414/94‑1 (MQ=60)

      ATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCAGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAA                                                                                         >  SRR4302533.3400999/1‑94 (MQ=60)

       TGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAAT                                                                                        <  SRR4302533.3114319/94‑1 (MQ=60)

       TGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAAT                                                                                        >  SRR4302533.3138239/1‑94 (MQ=60)

       TGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAAT                                                                                        <  SRR4302533.4505594/94‑1 (MQ=60)

        GGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATC                                                                                       <  SRR4302533.804887/94‑1 (MQ=60)

          AAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTT                                                                                     <  SRR4302533.5315439/94‑1 (MQ=60)

           AGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTT                                                                                    >  SRR4302533.1216152/1‑94 (MQ=60)

           AGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTT                                                                                    >  SRR4302533.4681098/1‑94 (MQ=60)

           AGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTT                                                                                    <  SRR4302533.6192179/94‑1 (MQ=60)

           AGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTT                                                                                    >  SRR4302533.2316998/1‑94 (MQ=60)

           AGAAATCAGCAAGTTTTTGCGGCGTCAGCAGGTTTATCTGGGCCACGATTTTATCACGCGCATCGAAGCGCATATTGCCGCGTTCGCAATCTTT                                                                                    >  SRR4302533.5428033/1‑94 (MQ=60)

            GAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTA                                                                                   >  SRR4302533.4066758/1‑94 (MQ=60)

            GAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTA                                                                                   <  SRR4302533.2425833/94‑1 (MQ=60)

                TCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTA                                                                               <  SRR4302533.269267/94‑1 (MQ=60)

                 CAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAA                                                                              >  SRR4302533.1381483/1‑94 (MQ=60)

                  AGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAAC                                                                             >  SRR4302533.4564407/1‑94 (MQ=60)

                   GCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACT                                                                            <  SRR4302533.5276539/94‑1 (MQ=60)

                   GCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACT                                                                            >  SRR4302533.1482086/1‑94 (MQ=60)

                   GCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACT                                                                            <  SRR4302533.4044678/94‑1 (MQ=60)

                    CAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTT                                                                           >  SRR4302533.5030009/1‑94 (MQ=60)

                     AAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTC                                                                          <  SRR4302533.716731/94‑1 (MQ=60)

                     AAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTC                                                                          <  SRR4302533.5171855/94‑1 (MQ=60)

                       GTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGA                                                                        >  SRR4302533.4324369/1‑94 (MQ=60)

                        TTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGAT                                                                       <  SRR4302533.3582175/94‑1 (MQ=60)

                          TTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGC                                                                     <  SRR4302533.5215022/94‑1 (MQ=60)

                          TTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGC                                                                     >  SRR4302533.314792/1‑94 (MQ=60)

                           TTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCT                                                                    <  SRR4302533.4326546/94‑1 (MQ=60)

                            TGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTT                                                                   >  SRR4302533.455843/1‑94 (MQ=60)

                            TGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTT                                                                   <  SRR4302533.2237861/94‑1 (MQ=60)

                             GCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTC                                                                  <  SRR4302533.5637044/94‑1 (MQ=60)

                             GCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTC                                                                  <  SRR4302533.5033592/94‑1 (MQ=60)

                              CGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCT                                                                 >  SRR4302533.3439635/1‑94 (MQ=60)

                              CGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCT                                                                 <  SRR4302533.3783266/94‑1 (MQ=60)

                              CGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCT                                                                 <  SRR4302533.3175779/94‑1 (MQ=60)

                              CGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCT                                                                 >  SRR4302533.2527768/1‑94 (MQ=60)

                              CGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCT                                                                 <  SRR4302533.4454221/94‑1 (MQ=60)

                               GGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTT                                                                >  SRR4302533.5428300/1‑94 (MQ=60)

                                GCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTC                                                               <  SRR4302533.351212/94‑1 (MQ=60)

                                GCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTC                                                               <  SRR4302533.5216163/94‑1 (MQ=60)

                                GCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTC                                                               <  SRR4302533.2713418/94‑1 (MQ=60)

                                 CGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCG                                                              <  SRR4302533.2860015/94‑1 (MQ=60)

                                  GTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGC                                                             >  SRR4302533.2124173/1‑94 (MQ=60)

                                  GTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGC                                                             <  SRR4302533.4684009/94‑1 (MQ=60)

                                  GTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGC                                                             >  SRR4302533.1104682/1‑94 (MQ=60)

                                   TCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCC                                                            >  SRR4302533.421286/1‑94 (MQ=60)

                                       CAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGC                                                        >  SRR4302533.3597137/1‑94 (MQ=60)

                                       CAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGC                                                        >  SRR4302533.491468/1‑94 (MQ=60)

                                        AGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCG                                                       >  SRR4302533.754738/1‑94 (MQ=60)

                                        AGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCG                                                       >  SRR4302533.2041297/1‑94 (MQ=60)

                                        AGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCG                                                       <  SRR4302533.6277854/94‑1 (MQ=60)

                                         GTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTTAATTTCATGCTTCTTCGCCGAGCGT                                                      >  SRR4302533.367288/1‑94 (MQ=60)

                                         GTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGT                                                      >  SRR4302533.3993117/1‑94 (MQ=60)

                                         GTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGT                                                      >  SRR4302533.1905902/1‑94 (MQ=60)

                                            TTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTT                                                   >  SRR4302533.3424882/1‑94 (MQ=60)

                                            TTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTG                                                   >  SRR4302533.3487385/1‑94 (MQ=60)

                                            TTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTG                                                   <  SRR4302533.5741515/94‑1 (MQ=60)

                                             TATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGC                                                  <  SRR4302533.6238103/94‑1 (MQ=60)

                                             TATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGC                                                  <  SRR4302533.5497727/94‑1 (MQ=60)

                                             TATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAAATTCGATGCTTCTTCGCCGAGCGTTTGC                                                  >  SRR4302533.1768263/1‑94 (MQ=60)

                                              ATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCG                                                 <  SRR4302533.1421803/94‑1 (MQ=60)

                                                CTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGT                                               <  SRR4302533.1281188/94‑1 (MQ=60)

                                                CTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGT                                               >  SRR4302533.5889422/1‑94 (MQ=60)

                                                CTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGT                                               <  SRR4302533.2428401/94‑1 (MQ=60)

                                                CTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGT                                               <  SRR4302533.5278708/94‑1 (MQ=60)

                                                 TGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTG                                              >  SRR4302533.5625779/1‑94 (MQ=60)

                                                 TGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTG                                              <  SRR4302533.5477934/94‑1 (MQ=60)

                                                 TGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTG                                              <  SRR4302533.4941312/94‑1 (MQ=60)

                                                  GGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGC                                             <  SRR4302533.3631263/94‑1 (MQ=60)

                                                   GGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCC                                            >  SRR4302533.4953428/1‑94 (MQ=60)

                                                    GCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCT                                           <  SRR4302533.3782452/94‑1 (MQ=60)

                                                    GCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCT                                           >  SRR4302533.4125130/1‑94 (MQ=60)

                                                      CACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGC                                         >  SRR4302533.5156988/1‑94 (MQ=60)

                                                        CGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAG                                       <  SRR4302533.5681213/94‑1 (MQ=60)

                                                        CGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAG                                       <  SRR4302533.4136220/94‑1 (MQ=60)

                                                         GATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGC                                      >  SRR4302533.5056638/1‑94 (MQ=60)

                                                          ATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCA                                     >  SRR4302533.5234924/1‑94 (MQ=60)

                                                          ATTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCA                                     >  SRR4302533.4201552/1‑94 (MQ=60)

                                                           TTTTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCAT                                    <  SRR4302533.2712704/94‑1 (MQ=60)

                                                             TTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCT                                  <  SRR4302533.21756/94‑1 (MQ=60)

                                                             TTATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCG                                  >  SRR4302533.561224/1‑94 (MQ=60)

                                                              TATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTG                                 >  SRR4302533.1902110/1‑94 (MQ=60)

                                                               ATCACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGG                                >  SRR4302533.6107491/1‑94 (MQ=60)

                                                                 CACGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGT                              >  SRR4302533.1577484/1‑94 (MQ=60)

                                                                   CGCGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAA                            <  SRR4302533.2882463/94‑1 (MQ=60)

                                                                     CGAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATT                          >  SRR4302533.2680717/1‑94 (MQ=60)

                                                                      GAATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTA                         <  SRR4302533.724981/94‑1 (MQ=60)

                                                                       AATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTAC                        >  SRR4302533.1973665/1‑94 (MQ=60)

                                                                       AATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTAC                        >  SRR4302533.856376/1‑94 (MQ=60)

                                                                        ATCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGGTTGCGGTGCCTGCAGCATCTGGGTAATTACC                       >  SRR4302533.3251768/1‑94 (MQ=60)

                                                                         TCGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCG                      <  SRR4302533.5957193/94‑1 (MQ=60)

                                                                          CGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGC                     <  SRR4302533.4215030/94‑1 (MQ=60)

                                                                          CGAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGC                     >  SRR4302533.4389688/1‑94 (MQ=60)

                                                                           GAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCC                    >  SRR4302533.2654669/1‑94 (MQ=60)

                                                                           GAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCC                    >  SRR4302533.6235580/1‑94 (MQ=60)

                                                                           GAAGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCC                    <  SRR4302533.4753827/94‑1 (MQ=60)

                                                                             AGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTG                  >  SRR4302533.5085671/1‑94 (MQ=60)

                                                                             AGCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTG                  >  SRR4302533.3286667/1‑94 (MQ=60)

                                                                              GCGCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGC                 >  SRR4302533.2394752/1‑94 (MQ=60)

                                                                                GCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTG               >  SRR4302533.4685887/1‑94 (MQ=60)

                                                                                GCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTG               >  SRR4302533.4311151/1‑94 (MQ=60)

                                                                                GCATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTG               >  SRR4302533.6112154/1‑94 (MQ=60)

                                                                                 CATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGG              >  SRR4302533.6070699/1‑94 (MQ=60)

                                                                                 CATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGG              >  SRR4302533.1398353/1‑94 (MQ=60)

                                                                                 CATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGG              >  SRR4302533.3413665/1‑94 (MQ=60)

                                                                                 CATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGG              <  SRR4302533.562830/94‑1 (MQ=60)

                                                                                 CATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGG              >  SRR4302533.3209809/1‑94 (MQ=60)

                                                                                  ATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGA             >  SRR4302533.199373/1‑94 (MQ=60)

                                                                                  ATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGA             >  SRR4302533.3198924/1‑94 (MQ=60)

                                                                                  ATATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGA             <  SRR4302533.6316890/94‑1 (MQ=60)

                                                                                   TATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGAT            <  SRR4302533.5765705/94‑1 (MQ=60)

                                                                                   TATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGAT            >  SRR4302533.5024197/1‑94 (MQ=60)

                                                                                    ATTGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTAGATT           >  SRR4302533.1490760/1‑94 (MQ=60)

                                                                                      TGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTG         >  SRR4302533.2480605/1‑94 (MQ=60)

                                                                                      TGCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTG         <  SRR4302533.1384949/94‑1 (MQ=60)

                                                                                       GCCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGC        <  SRR4302533.3523296/94‑1 (MQ=60)

                                                                                        CCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCG       >  SRR4302533.4337174/1‑94 (MQ=60)

                                                                                        CCGCGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGGAATTACCGCCTGCTGGATTTGCG       <  SRR4302533.4156004/94‑1 (MQ=60)

                                                                                           CGTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCGCAA    <  SRR4302533.531032/94‑1 (MQ=60)

                                                                                            GTTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCGCAAA   >  SRR4302533.5769/1‑94 (MQ=60)

                                                                                             TTCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCGCAAAC  >  SRR4302533.5725957/1‑94 (MQ=60)

CGCCTGATGGAAGAAATCAGCAAGTTTTTGCGGCGTCAGCAGTTTTATCTGGGCCACGATTTTATCACGCGAATCGAAGCGCATATTGCCGCGATCGAAATCTTTACTTAACTTCGATGCTTCTTCGCCGAGCGTTTGCGGTGCCTGCAGCATCTGGGTAATTACCGCCTGCTGGATTTGCGCAAAC  >  NC_000913/2954171‑2954357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`