Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,992	A→G	E117G (GAG→GGG)	wzzE →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,992	0	A	G	99.6%	808.9 / NA	251	E117G (GAG→GGG)	wzzE	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (139/111); minor base C (1/0); total (140/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA  >  NC_000913/3966902‑3967081

 aaaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGtt                                                                                        >  2:2672110/1‑93 (MQ=255)

 aaaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGtt                                                                                        >  1:5008354/1‑93 (MQ=255)

  aaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTc                                                                                       >  1:3310309/1‑93 (MQ=255)

  aaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTc                                                                                       >  1:3228755/1‑93 (MQ=255)

  aaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTc                                                                                       >  2:2201647/1‑93 (MQ=255)

   aCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCt                                                                                      >  1:1179715/1‑93 (MQ=255)

   aCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCt                                                                                      <  1:463718/93‑1 (MQ=255)

    cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTg                                                                                     <  2:5468427/93‑1 (MQ=255)

    cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTg                                                                                     >  2:1721987/1‑93 (MQ=255)

          ttCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCa                                                                               >  1:2394260/1‑93 (MQ=255)

           tCTGCCGACCAACCATCGGTCTTGGATGTTGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACTCGCAGAGGTTTCTGGCTGCaa                                                                              >  1:6309630/1‑93 (MQ=255)

           tCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCaa                                                                              <  2:3913295/93‑1 (MQ=255)

             tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCTACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAc                                                                            <  1:3391960/93‑1 (MQ=255)

             tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAc                                                                            <  2:2919067/93‑1 (MQ=255)

             tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAc                                                                            <  1:2343799/93‑1 (MQ=255)

              gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAcc                                                                           >  2:1038036/1‑93 (MQ=255)

              gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAcc                                                                           <  1:1127675/93‑1 (MQ=255)

                 gACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGAc                                                                        >  2:4604996/1‑93 (MQ=255)

                 gACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGAc                                                                        <  1:3663784/93‑1 (MQ=255)

                 gACCAACCATCGGTCATGGACGAAGCCTATAAAGAGATTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGATCTGGCTGCAAACCGAc                                                                        >  2:928402/1‑93 (MQ=255)

                    caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTAt                                                                     >  1:6221247/1‑93 (MQ=255)

                    caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTAt                                                                     <  2:4770241/93‑1 (MQ=255)

                      accaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTa                                                                   >  2:5076226/1‑93 (MQ=255)

                        caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACa                                                                 >  2:444014/1‑93 (MQ=255)

                         aTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACaa                                                                >  2:5544240/1‑93 (MQ=255)

                         aTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACaa                                                                >  2:4557153/1‑93 (MQ=255)

                          tCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACaaa                                                               <  1:477321/93‑1 (MQ=255)

                             gTCATGGACGAAGCCTATAAAGAGGTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAg                                                            >  2:5341108/1‑93 (MQ=255)

                              tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGc                                                           >  2:3763719/1‑93 (MQ=255)

                              tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGc                                                           >  2:2592616/1‑93 (MQ=255)

                              tCATGGACGAAGCCTATAAAGAGCTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGc                                                           >  2:4069100/1‑93 (MQ=255)

                               cATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCg                                                          >  1:2632991/1‑93 (MQ=255)

                               cATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCg                                                          >  1:1752402/1‑93 (MQ=255)

                                aTGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCgg                                                         >  2:654396/1‑93 (MQ=255)

                                aTGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCgg                                                         >  1:5352399/1‑93 (MQ=255)

                                aTGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCgg                                                         >  1:5695663/1‑93 (MQ=255)

                                 tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGa                                                        >  2:5910055/1‑93 (MQ=255)

                                 tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGa                                                        >  1:2933003/1‑93 (MQ=255)

                                 tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGa                                                        >  1:5512389/1‑93 (MQ=255)

                                  ggACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAt                                                       >  1:4435694/1‑93 (MQ=255)

                                   gACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtg                                                      >  2:3576009/1‑93 (MQ=255)

                                      gAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtggtg                                                   >  1:4390647/1‑93 (MQ=255)

                                      gAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtggtg                                                   >  1:4003180/1‑93 (MQ=255)

                                       aaGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtggtgg                                                  >  1:5100215/1‑93 (MQ=255)

                                       aaGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtggtgg                                                  <  2:3506154/93‑1 (MQ=255)

                                         gCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGc                                                >  1:757738/1‑93 (MQ=255)

                                         gCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGCGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGc                                                >  2:5297698/1‑93 (MQ=255)

                                           cTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCaa                                              >  1:3619447/1‑93 (MQ=255)

                                           cTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCaa                                              >  1:3491334/1‑93 (MQ=255)

                                            tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCaaa                                             >  1:1994107/1‑92 (MQ=255)

                                            tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAAc                                             <  1:2208280/93‑1 (MQ=255)

                                            tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAAc                                             >  2:134849/1‑93 (MQ=255)

                                              taAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAg                                           >  1:5947488/1‑93 (MQ=255)

                                              taAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAg                                           >  1:2465316/1‑93 (MQ=255)

                                                 agagTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCaa                                        <  2:1071356/93‑1 (MQ=255)

                                                  gagTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCaaa                                       >  2:4040514/1‑93 (MQ=255)

                                                  gagTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCaaa                                       <  2:3675852/93‑1 (MQ=255)

                                                   agTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAg                                      <  2:1621365/93‑1 (MQ=255)

                                                    gTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGc                                     <  1:2676592/93‑1 (MQ=255)

                                                    gTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGc                                     <  1:3884314/93‑1 (MQ=255)

                                                       tGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGTTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGa                                  <  2:4487243/93‑1 (MQ=255)

                                                       tGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTATAAACAGCGGATGGTGGGCAACAGCAAAGCCGa                                  <  1:5849759/93‑1 (MQ=255)

                                                        gTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAt                                 >  2:4970487/1‑93 (MQ=255)

                                                        gTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAt                                 <  1:894962/93‑1 (MQ=255)

                                                        gTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAt                                 <  2:3977561/93‑1 (MQ=255)

                                                        gTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAt                                 <  1:185638/93‑1 (MQ=255)

                                                         ttATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGGCGATg                                >  2:2559405/1‑93 (MQ=255)

                                                         ttATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATg                                >  1:3688309/1‑93 (MQ=255)

                                                          tATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgc                               >  1:4125994/1‑93 (MQ=255)

                                                          tATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgc                               <  2:55956/93‑1 (MQ=255)

                                                               aaCTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGt                          >  1:167890/1‑93 (MQ=255)

                                                                aCTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGtt                         >  2:414415/1‑93 (MQ=255)

                                                                  tGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGc                       <  1:1537155/93‑1 (MQ=255)

                                                                    gCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTg                     >  1:4324406/1‑93 (MQ=255)

                                                                    gCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTg                     <  1:4716065/93‑1 (MQ=255)

                                                                    gCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTg                     <  2:4263837/93‑1 (MQ=255)

                                                                     ccTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTgg                    <  2:6002863/93‑1 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCACCAGCAAAGCCGATGCGGCGTTGCTGGAt                  >  2:6214438/1‑93 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCt                      >  2:240746/1‑89 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  >  1:2040968/1‑93 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  >  1:6167654/1‑93 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  <  2:2616051/93‑1 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  >  2:2059119/1‑93 (MQ=255)

                                                                        cGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                 >  2:6119783/1‑93 (MQ=255)

                                                                         gTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGa                >  2:5755975/1‑93 (MQ=255)

                                                                         gTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGa                >  2:2153235/1‑93 (MQ=255)

                                                                          tGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa               >  2:771455/1‑93 (MQ=255)

                                                                          tGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa               >  1:1104978/1‑93 (MQ=255)

                                                                          tGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa               <  2:342671/93‑1 (MQ=255)

                                                                          tGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa               >  2:1226122/1‑93 (MQ=255)

                                                                            ggATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAt             >  1:4215617/1‑93 (MQ=255)

                                                                            ggATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAt             <  2:4382902/93‑1 (MQ=255)

                                                                            ggATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAt             <  2:6153110/93‑1 (MQ=255)

                                                                              aTACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa           <  1:5244906/93‑1 (MQ=255)

                                                                              aTACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa           >  2:1692190/1‑93 (MQ=255)

                                                                               tACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAt          <  2:863514/93‑1 (MQ=255)

                                                                                 cccGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa        >  1:4608919/1‑93 (MQ=255)

                                                                                   cGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaac      >  1:1033194/1‑93 (MQ=255)

                                                                                      agagggTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaacaac   >  1:3468450/1‑93 (MQ=255)

                                                                                      agagggTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaacaac   >  2:3017394/1‑93 (MQ=255)

CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA  >  NC_000913/3966902‑3967081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCA  >  NC_000913/3966899‑3967085

GTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGTCCTCGTGGGATACCCGCAGAGG                                                                                               >  SRR4302533.2865037/1‑94 (MQ=60)

 TTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGG                                                                                              <  SRR4302533.1737484/94‑1 (MQ=60)

   CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTT                                                                                            >  SRR4302533.5038701/1‑94 (MQ=60)

   CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTT                                                                                            >  SRR4302533.417926/1‑94 (MQ=60)

   CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTT                                                                                            <  SRR4302533.2641257/94‑1 (MQ=60)

   CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTT                                                                                            <  SRR4302533.1910198/94‑1 (MQ=60)

    AAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTC                                                                                           >  SRR4302533.3330749/1‑94 (MQ=60)

    AAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTC                                                                                           >  SRR4302533.3248659/1‑94 (MQ=60)

     AACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCT                                                                                          >  SRR4302533.1186803/1‑94 (MQ=60)

     AACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCT                                                                                          <  SRR4302533.3691150/94‑1 (MQ=60)

     AACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCT                                                                                          <  SRR4302533.3447656/94‑1 (MQ=60)

      ACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTG                                                                                         >  SRR4302533.946060/1‑94 (MQ=60)

      ACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTG                                                                                         <  SRR4302533.684671/94‑1 (MQ=60)

      ACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTG                                                                                         <  SRR4302533.466486/94‑1 (MQ=60)

      ACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTG                                                                                         <  SRR4302533.573626/94‑1 (MQ=60)

      ACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTG                                                                                         >  SRR4302533.1782194/1‑94 (MQ=60)

        ATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGC                                                                                       >  SRR4302533.6293839/1‑94 (MQ=60)

        ATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGC                                                                                       >  SRR4302533.6091357/1‑94 (MQ=60)

            CTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCA                                                                                   <  SRR4302533.3694052/94‑1 (MQ=60)

            CTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCA                                                                                   <  SRR4302533.4418210/94‑1 (MQ=60)

            CTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCA                                                                                   >  SRR4302533.2409039/1‑94 (MQ=60)

             TTCTGCCGACCAACCATCGGTCTTGGATGTTGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACTCGCAGAGGTTTCTGGCTGCAA                                                                                  >  SRR4302533.6347579/1‑94 (MQ=60)

               CTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAC                                                                                >  SRR4302533.1127679/1‑94 (MQ=60)

               CTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAC                                                                                <  SRR4302533.5644189/94‑1 (MQ=60)

                TGGCGACCAACCTTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATGCCCGCAGAGGGTTCTGGCTGCAAACC                                                                               <  SRR4302533.3529597/94‑1 (MQ=60)

                TGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCTACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACC                                                                               <  SRR4302533.3412908/94‑1 (MQ=60)

                TGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACC                                                                               >  SRR4302533.407292/1‑94 (MQ=60)

                TGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACC                                                                               <  SRR4302533.3157851/94‑1 (MQ=60)

                TGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACC                                                                               <  SRR4302533.2358307/94‑1 (MQ=60)

                 GCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCG                                                                              <  SRR4302533.1134375/94‑1 (MQ=60)

                 GCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCG                                                                              <  SRR4302533.3511687/94‑1 (MQ=60)

                  CCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGA                                                                             >  SRR4302533.5774133/1‑94 (MQ=60)

                    GACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACT                                                                           <  SRR4302533.3686305/94‑1 (MQ=60)

                      CCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTAT                                                                         >  SRR4302533.6258538/1‑94 (MQ=60)

                       CAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATT                                                                        <  SRR4302533.355159/94‑1 (MQ=60)

                       CAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATT                                                                        >  SRR4302533.285460/1‑94 (MQ=60)

                          CCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACA                                                                     >  SRR4302533.2387123/1‑94 (MQ=60)

                           CATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAA                                                                    >  SRR4302533.4372394/1‑94 (MQ=60)

                           CATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAA                                                                    >  SRR4302533.1880188/1‑94 (MQ=60)

                           CATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTCCAAACCGACTATTACAA                                                                    >  SRR4302533.4479627/1‑94 (MQ=60)

                             TCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAAC                                                                  <  SRR4302533.480170/94‑1 (MQ=60)

                              CGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACA                                                                 >  SRR4302533.5515602/1‑94 (MQ=60)

                               GGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTAAAAACAG                                                                >  SRR4302533.2028959/1‑94 (MQ=60)

                                 TCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCG                                                              >  SRR4302533.1763077/1‑94 (MQ=60)

                                 TCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCG                                                              <  SRR4302533.3354900/94‑1 (MQ=60)

                                 TCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCG                                                              >  SRR4302533.2649171/1‑94 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                             >  SRR4302533.5384518/1‑94 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                             >  SRR4302533.5730072/1‑94 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                             >  SRR4302533.4769136/1‑94 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                             >  SRR4302533.4405611/1‑94 (MQ=60)

                                   ATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGA                                                            >  SRR4302533.2950939/1‑94 (MQ=60)

                                   ATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGA                                                            >  SRR4302533.5545666/1‑94 (MQ=60)

                                    TGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAT                                                           >  SRR4302533.6250801/1‑94 (MQ=60)

                                    TGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAT                                                           >  SRR4302533.4462509/1‑94 (MQ=60)

                                      GACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGG                                                         <  SRR4302533.3742555/94‑1 (MQ=60)

                                       ACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGT                                                        >  SRR4302533.5163106/1‑94 (MQ=60)

                                        CGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTG                                                       >  SRR4302533.4027556/1‑94 (MQ=60)

                                        CGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTG                                                       >  SRR4302533.4417155/1‑94 (MQ=60)

                                        CGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTG                                                       >  SRR4302533.5896431/1‑94 (MQ=60)

                                        CGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTG                                                       >  SRR4302533.4128540/1‑94 (MQ=60)

                                         GAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGG                                                      >  SRR4302533.5131025/1‑94 (MQ=60)

                                          AAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGG                                                     <  SRR4302533.4969799/94‑1 (MQ=60)

                                          AAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGG                                                     >  SRR4302533.3652568/1‑94 (MQ=60)

                                           AGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGC                                                    >  SRR4302533.762199/1‑94 (MQ=60)

                                             CCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAA                                                  >  SRR4302533.3512867/1‑94 (MQ=60)

                                             CCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAA                                                  >  SRR4302533.3966882/1‑94 (MQ=60)

                                             CCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAA                                                  >  SRR4302533.3641715/1‑94 (MQ=60)

                                              CTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAAA                                                 >  SRR4302533.2006341/1‑94 (MQ=60)

                                               TATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACA                                                <  SRR4302533.2221900/94‑1 (MQ=60)

                                               TATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACA                                                >  SRR4302533.465669/1‑94 (MQ=60)

                                               TATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACA                                                <  SRR4302533.836305/94‑1 (MQ=60)

                                                ATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAG                                               >  SRR4302533.2480523/1‑94 (MQ=60)

                                                ATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAG                                               >  SRR4302533.5983461/1‑94 (MQ=60)

                                                ATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAG                                               <  SRR4302533.213310/94‑1 (MQ=60)

                                                      AGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGC                                         <  SRR4302533.5292179/94‑1 (MQ=60)

                                                       GTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAGCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCC                                        <  SRR4302533.6250325/94‑1 (MQ=60)

                                                       GTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCC                                        <  SRR4302533.2291234/94‑1 (MQ=60)

                                                       GTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCC                                        <  SRR4302533.3908014/94‑1 (MQ=60)

                                                       GTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCC                                        <  SRR4302533.2693004/94‑1 (MQ=60)

                                                         TTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGA                                      >  SRR4302533.1065102/1‑94 (MQ=60)

                                                          TGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTATAAACAGCGGATGGTGGGCAACAGCAAAGCCGAT                                     <  SRR4302533.5885128/94‑1 (MQ=60)

                                                          TGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAT                                     <  SRR4302533.3313801/94‑1 (MQ=60)

                                                          TGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAT                                     >  SRR4302533.1505308/1‑94 (MQ=60)

                                                           GTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATG                                    <  SRR4302533.186793/94‑1 (MQ=60)

                                                           GTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATG                                    <  SRR4302533.900220/94‑1 (MQ=60)

                                                           GTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATG                                    >  SRR4302533.3710975/1‑94 (MQ=60)

                                                            TTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGC                                   >  SRR4302533.4151039/1‑94 (MQ=60)

                                                              ATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGG                                 >  SRR4302533.1118613/1‑94 (MQ=60)

                                                                GCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCG                               <  SRR4302533.1167517/94‑1 (MQ=60)

                                                                GCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCG                               >  SRR4302533.5339031/1‑94 (MQ=60)

                                                                 CAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGT                              >  SRR4302533.168951/1‑94 (MQ=60)

                                                                 CAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGT                              >  SRR4302533.2192005/1‑94 (MQ=60)

                                                                 CAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGT                              >  SRR4302533.4709052/1‑94 (MQ=60)

                                                                 CAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGT                              <  SRR4302533.4706899/94‑1 (MQ=60)

                                                                  AACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTT                             >  SRR4302533.2429614/1‑94 (MQ=60)

                                                                  AACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTT                             <  SRR4302533.227977/94‑1 (MQ=60)

                                                                   ACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTG                            <  SRR4302533.5539171/94‑1 (MQ=60)

                                                                   ACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTG                            <  SRR4302533.3335010/94‑1 (MQ=60)

                                                                    CTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGC                           >  SRR4302533.3480766/1‑94 (MQ=60)

                                                                    CTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGC                           >  SRR4302533.4890622/1‑94 (MQ=60)

                                                                     TGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCT                          <  SRR4302533.1247933/94‑1 (MQ=60)

                                                                     TGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCT                          <  SRR4302533.1546389/94‑1 (MQ=60)

                                                                      tatCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTG                         <  SRR4302533.242240/91‑1 (MQ=60)

                                                                      GGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTG                         >  SRR4302533.1816855/1‑94 (MQ=60)

                                                                      GGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTG                         <  SRR4302533.1618834/94‑1 (MQ=60)

                                                                      GGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTG                         <  SRR4302533.1735473/94‑1 (MQ=60)

                                                                      GGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTG                         >  SRR4302533.2664989/1‑94 (MQ=60)

                                                                      GGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTG                         >  SRR4302533.4350448/1‑94 (MQ=60)

                                                                       GCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGG                        <  SRR4302533.4744652/94‑1 (MQ=60)

                                                                       GCCTCGTGGGATACCCGCAGAGGATTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGG                        <  SRR4302533.82291/94‑1 (MQ=60)

                                                                         CTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAT                      >  SRR4302533.3021052/1‑94 (MQ=60)

                                                                         CTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAT                      >  SRR4302533.6204731/1‑94 (MQ=60)

                                                                         CTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAT                      >  SRR4302533.2053546/1‑94 (MQ=60)

                                                                          TCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATG                     <  SRR4302533.4662373/94‑1 (MQ=60)

                                                                           CGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGA                    >  SRR4302533.4170706/1‑94 (MQ=60)

                                                                           CGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCAACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGA                    >  SRR4302533.4277035/1‑94 (MQ=60)

                                                                            GTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAA                   >  SRR4302533.277864/1‑94 (MQ=60)

                                                                            GTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAA                   <  SRR4302533.1646748/94‑1 (MQ=60)

                                                                            GTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAA                   >  SRR4302533.1111526/1‑94 (MQ=60)

                                                                             TGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAGACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAA                  >  SRR4302533.4459407/1‑94 (MQ=60)

                                                                              GGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAT                 <  SRR4302533.6126815/94‑1 (MQ=60)

                                                                              GGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAT                 >  SRR4302533.5092319/1‑94 (MQ=60)

                                                                              GGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAT                 >  SRR4302533.4241119/1‑94 (MQ=60)

                                                                                GATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGA               <  SRR4302533.3856232/94‑1 (MQ=60)

                                                                                 ATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAT              <  SRR4302533.5276429/94‑1 (MQ=60)

                                                                                 ATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAT              <  SRR4302533.511366/94‑1 (MQ=60)

                                                                                   ACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTA            <  SRR4302533.737036/94‑1 (MQ=60)

                                                                                   ACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTA            >  SRR4302533.4636833/1‑94 (MQ=60)

                                                                                     CCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAAC          >  SRR4302533.1039271/1‑94 (MQ=60)

                                                                                        CAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAAC       >  SRR4302533.3489860/1‑94 (MQ=60)

                                                                                         AGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA      >  SRR4302533.2568300/1‑94 (MQ=60)

                                                                                          GAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACAT     >  SRR4302533.1927742/1‑94 (MQ=60)

                                                                                           AGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATC    <  SRR4302533.2157381/94‑1 (MQ=60)

                                                                                           AGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATC    >  SRR4302533.2252638/1‑94 (MQ=60)

                                                                                           AGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATC    >  SRR4302533.481771/1‑94 (MQ=60)

                                                                                           AGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATC    <  SRR4302533.444093/94‑1 (MQ=60)

                                                                                            GGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCC   >  SRR4302533.5300507/1‑94 (MQ=60)

                                                                                             GGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCA  >  SRR4302533.1942536/1‑94 (MQ=60)

                                                                                             GGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCA  >  SRR4302533.5058887/1‑94 (MQ=60)

                                                                                             GGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCA  >  SRR4302533.330627/1‑94 (MQ=60)

GTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCA  >  NC_000913/3966899‑3967085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`