Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,973,216	C→T	A153A (GCC→GCT)	wecE →	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,973,216	0	C	T	100.0%	878.5 / NA	234	A153A (GCC→GCT)	wecE	putative regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (123/111); total (123/111)

Only 100 of 247 total aligned reads displayed.

CGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTA  >  NC_000913/3973126‑3973305

cGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGAtgctgc                                                                                         <  2:2012210/93‑1 (MQ=255)

   gCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCa                                                                                      <  1:911472/93‑1 (MQ=255)

     cGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAgg                                                                                    <  1:437561/93‑1 (MQ=255)

     cGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAgg                                                                                    <  2:1350889/93‑1 (MQ=255)

     cGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAgg                                                                                    <  2:5959535/93‑1 (MQ=255)

      ggTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAggg                                                                                   >  2:3822009/1‑93 (MQ=255)

      ggTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAggg                                                                                   >  1:4081435/1‑93 (MQ=255)

         ccATTACGCGGGTTTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGt                                                                                <  1:177257/93‑1 (MQ=255)

         ccATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGt                                                                                <  2:2036273/93‑1 (MQ=255)

         ccATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGt                                                                                <  2:1325310/93‑1 (MQ=255)

          cATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTg                                                                               <  2:4622306/93‑1 (MQ=255)

           aTTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGCAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCCGGGCGGGa                                                                              >  1:863039/1‑93 (MQ=255)

           aTTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGTTAGAAGATGCTGCTCAGGGCGTGa                                                                              <  1:2359564/93‑1 (MQ=255)

           aTTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCTAGGCGTGa                                                                              >  1:6030257/1‑93 (MQ=255)

           aTTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGa                                                                              >  1:2448721/1‑93 (MQ=255)

            ttACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGAt                                                                             <  2:581867/93‑1 (MQ=255)

               cgcgGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTc                                                                          <  1:1859037/93‑1 (MQ=255)

                  gggTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCAc                                                                       >  1:1710002/1‑93 (MQ=255)

                   ggTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACt                                                                      <  1:1920481/93‑1 (MQ=255)

                     tgtgGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTa                                                                    >  1:3741438/1‑93 (MQ=255)

                      gtgGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTAc                                                                   <  2:564896/93‑1 (MQ=255)

                          ccTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAg                                                               <  2:358391/93‑1 (MQ=255)

                           cTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAgg                                                              >  1:750829/1‑93 (MQ=255)

                           cTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAgg                                                              <  2:1707080/93‑1 (MQ=255)

                            tGCGAAATGGACAGCATTATGGCGTTGTCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAggg                                                             <  2:1488536/93‑1 (MQ=255)

                            tGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAggg                                                             >  1:5001700/1‑93 (MQ=255)

                               gAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGt                                                          <  1:453321/93‑1 (MQ=255)

                               gAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGt                                                          >  2:2404848/1‑93 (MQ=255)

                                 aaTGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGc                                                        <  1:5776455/93‑1 (MQ=255)

                                 aaTGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGc                                                        >  2:191555/1‑93 (MQ=255)

                                   tGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCAc                                                      <  2:2166037/93‑1 (MQ=255)

                                    ggACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACt                                                     >  1:1401883/1‑93 (MQ=255)

                                    ggACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACt                                                     >  2:5035090/1‑93 (MQ=255)

                                    ggACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACt                                                     >  1:3476137/1‑93 (MQ=255)

                                     gACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTg                                                    <  1:5332270/93‑1 (MQ=255)

                                       cacCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTggg                                                  >  1:3522659/1‑93 (MQ=255)

                                       cacCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTggg                                                  <  1:2973381/93‑1 (MQ=255)

                                         ccATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGaa                                                <  1:4900389/93‑1 (MQ=255)

                                         ccATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGaa                                                >  1:567864/1‑93 (MQ=255)

                                         ccATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGaa                                                <  2:1493496/93‑1 (MQ=255)

                                          cATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACACAGGGCGTGCACTGGGAAc                                               >  1:781551/1‑93 (MQ=255)

                                            ttATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCa                                             >  1:5180635/1‑93 (MQ=255)

                                            ttATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCa                                             >  1:3263232/1‑93 (MQ=255)

                                            ttATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCa                                             >  2:1650218/1‑93 (MQ=255)

                                            ttATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCa                                             >  2:4490406/1‑93 (MQ=255)

                                            ttATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCa                                             <  2:5293738/93‑1 (MQ=255)

                                               tGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCAGTTACAAAGGGCGTGCACTGGGAACCATTg                                          >  2:6191944/1‑93 (MQ=255)

                                               tGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTg                                          >  2:66591/1‑93 (MQ=255)

                                               tGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTg                                          <  2:1001684/93‑1 (MQ=255)

                                                ggCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTgg                                         >  2:3318868/1‑93 (MQ=255)

                                                ggCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTgg                                         >  2:8220/1‑93 (MQ=255)

                                                ggCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTgg                                         >  1:1318174/1‑93 (MQ=255)

                                                 gCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGt                                        <  1:1006213/93‑1 (MQ=255)

                                                  cGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTc                                       >  2:289333/1‑93 (MQ=255)

                                                   gTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCa                                      >  1:2595586/1‑93 (MQ=255)

                                                    ttGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCat                                     >  2:487977/1‑93 (MQ=255)

                                                    ttGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCat                                     <  1:2828392/93‑1 (MQ=255)

                                                    ttGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCat                                     >  2:4345329/1‑93 (MQ=255)

                                                         gAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGTGCGTGCACTGGGAACCATTGGTCATATTgg                                >  1:6257385/1‑93 (MQ=255)

                                                         gAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTgg                                <  2:1109532/93‑1 (MQ=255)

                                                          aaaaaGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGgc                               >  1:683063/1‑93 (MQ=255)

                                                          aaaaaGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGgc                               >  2:2307693/1‑93 (MQ=255)

                                                          aaaaaGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGgc                               >  1:3976555/1‑93 (MQ=255)

                                                          aaaaaGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGgc                               >  1:1246038/1‑93 (MQ=255)

                                                          aaaaaGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGgc                               >  1:2102991/1‑93 (MQ=255)

                                                            aaaGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGgctg                             >  1:478425/1‑93 (MQ=255)

                                                            aaaGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGgctg                             <  2:1762652/93‑1 (MQ=255)

                                                               gCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCtt                          >  1:1127295/1‑93 (MQ=255)

                                                               gCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCtt                          >  1:5887302/1‑93 (MQ=255)

                                                               gCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCtt                          >  2:61132/1‑93 (MQ=255)

                                                                cATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCttt                         <  2:2185811/93‑1 (MQ=255)

                                                                cATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCttt                         <  2:5998248/93‑1 (MQ=255)

                                                                cATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCttt                         <  1:1298895/93‑1 (MQ=255)

                                                                 aTAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTa                        >  1:3626895/1‑93 (MQ=255)

                                                                 aTAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTa                        <  1:5983984/93‑1 (MQ=255)

                                                                  tAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAg                       <  2:3432090/93‑1 (MQ=255)

                                                                   aaTTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGc                      <  2:1188163/93‑1 (MQ=255)

                                                                   aaTTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGc                      <  1:2308434/93‑1 (MQ=255)

                                                                    aTTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCt                     >  1:4790083/1‑93 (MQ=255)

                                                                    aTTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCt                     >  1:6014769/1‑93 (MQ=255)

                                                                       tgtttgtGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTcc                  <  1:3159895/93‑1 (MQ=255)

                                                                       tgtttgtGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTcc                  >  2:4905420/1‑93 (MQ=255)

                                                                        gtttgtGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCa                 >  1:3052720/1‑93 (MQ=255)

                                                                        gtttgtGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCa                 <  1:59270/93‑1 (MQ=255)

                                                                         tttgtGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCAt                >  2:5226192/1‑93 (MQ=255)

                                                                         tttgtGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCAt                <  2:2374502/93‑1 (MQ=255)

                                                                            gtgGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGaa             >  2:3330679/1‑93 (MQ=255)

                                                                               gTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAAcc          <  2:4000248/93‑1 (MQ=255)

                                                                               gTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAAcc          >  2:4244350/1‑93 (MQ=255)

                                                                                  gaagaTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCaaa       >  2:3918738/1‑93 (MQ=255)

                                                                                  gaagaTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAAACaaa       >  1:5106407/1‑93 (MQ=255)

                                                                                    agaTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCaaaaa     <  1:1565522/93‑1 (MQ=255)

                                                                                    agaTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCaaaaa     <  2:3148052/93‑1 (MQ=255)

                                                                                     gaTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAAc    >  2:4992625/1‑93 (MQ=255)

                                                                                     gaTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAAc    <  2:4664063/93‑1 (MQ=255)

                                                                                     gaTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAAc    >  2:308907/1‑93 (MQ=255)

                                                                                      aTGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACt   >  1:5252368/1‑93 (MQ=255)

                                                                                       tgctgcTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTa  >  2:2810992/1‑93 (MQ=255)

                                                                                       tgctgcTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTa  <  2:2716949/93‑1 (MQ=255)

                                                                                       tgctgcTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTa  <  1:4214215/93‑1 (MQ=255)

CGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTA  >  NC_000913/3973126‑3973305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACG  >  NC_000913/3973124‑3973309

ATCGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTG                                                                                              >  SRR4302533.4932669/1‑94 (MQ=60)

ATCGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTG                                                                                              >  SRR4302533.332681/1‑94 (MQ=60)

 TCGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGC                                                                                             <  SRR4302533.3325301/94‑1 (MQ=60)

 TCGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGC                                                                                             >  SRR4302533.3693823/1‑94 (MQ=60)

  CGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCT                                                                                            <  SRR4302533.4775686/94‑1 (MQ=60)

    TGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCA                                                                                          <  SRR4302533.2350379/94‑1 (MQ=60)

     GCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAG                                                                                         <  SRR4302533.916819/94‑1 (MQ=60)

      CCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGG                                                                                        <  SRR4302533.4340397/94‑1 (MQ=60)

       CGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGG                                                                                       >  SRR4302533.1920103/1‑94 (MQ=60)

       CGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGG                                                                                       <  SRR4302533.1464661/94‑1 (MQ=60)

       CGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGG                                                                                       >  SRR4302533.4106281/1‑94 (MQ=60)

       CGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGG                                                                                       <  SRR4302533.440168/94‑1 (MQ=60)

       CGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGG                                                                                       <  SRR4302533.5002398/94‑1 (MQ=60)

       CGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGG                                                                                       <  SRR4302533.98478/94‑1 (MQ=60)

        GGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGC                                                                                      >  SRR4302533.127943/1‑94 (MQ=60)

         GTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCG                                                                                     <  SRR4302533.5961296/94‑1 (MQ=60)

          TCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGT                                                                                    >  SRR4302533.3254785/1‑94 (MQ=60)

          TCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGT                                                                                    >  SRR4302533.4075041/1‑94 (MQ=60)

           CCATTACGCGGGTTTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTG                                                                                   <  SRR4302533.178367/94‑1 (MQ=60)

           CCATTACGCGGGTGTGGCCTGCGAAATGGACAGCATTATGGCGTTGGCGAAAAAGCATAATGTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTG                                                                                   <  SRR4302533.3241129/94‑1 (MQ=60)

            CATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGCAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCCGGGCGGGA                                                                                  >  SRR4302533.868097/1‑94 (MQ=60)

            CATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCTAGGCGTGA                                                                                  >  SRR4302533.6066803/1‑94 (MQ=60)

            CATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGA                                                                                  >  SRR4302533.2463836/1‑94 (MQ=60)

             ATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGTTAGAAGATGCTGCTCAGGGCGTGAT                                                                                 <  SRR4302533.2374147/94‑1 (MQ=60)

             ATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGAT                                                                                 >  SRR4302533.821275/1‑94 (MQ=60)

             ATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCCGCTCAGGGCGTGAT                                                                                 >  SRR4302533.5381962/1‑94 (MQ=60)

              TTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATG                                                                                <  SRR4302533.2395426/94‑1 (MQ=60)

                 CGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCC                                                                             <  SRR4302533.1870372/94‑1 (MQ=60)

                   CGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCAC                                                                           >  SRR4302533.1720365/1‑94 (MQ=60)

                   CGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCAC                                                                           <  SRR4302533.1392285/94‑1 (MQ=60)

                     GGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTT                                                                         <  SRR4302533.1932205/94‑1 (MQ=60)

                      GTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTA                                                                        >  SRR4302533.3764381/1‑94 (MQ=60)

                        GTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACA                                                                      >  SRR4302533.1981180/1‑94 (MQ=60)

                           GCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAG                                                                   <  SRR4302533.3755405/94‑1 (MQ=60)

                            CCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGG                                                                  >  SRR4302533.755250/1‑94 (MQ=60)

                            CCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGG                                                                  <  SRR4302533.5187869/94‑1 (MQ=60)

                             CTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGG                                                                 <  SRR4302533.6193814/94‑1 (MQ=60)

                             CTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGG                                                                 >  SRR4302533.5032020/1‑94 (MQ=60)

                              TGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGC                                                                <  SRR4302533.107595/94‑1 (MQ=60)

                              TGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGC                                                                <  SRR4302533.5210233/94‑1 (MQ=60)

                                CGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGT                                                              >  SRR4302533.5750898/1‑94 (MQ=60)

                                 GAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTG                                                             <  SRR4302533.456032/94‑1 (MQ=60)

                                   AATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCA                                                           <  SRR4302533.4815465/94‑1 (MQ=60)

                                   AATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCA                                                           >  SRR4302533.1373212/1‑94 (MQ=60)

                                   AATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCA                                                           <  SRR4302533.4549971/94‑1 (MQ=60)

                                   AATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCA                                                           <  SRR4302533.5896760/94‑1 (MQ=60)

                                   AATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCA                                                           <  SRR4302533.5811342/94‑1 (MQ=60)

                                     TGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACT                                                         >  SRR4302533.1410337/1‑94 (MQ=60)

                                     TGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACT                                                         >  SRR4302533.3497587/1‑94 (MQ=60)

                                      GGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTG                                                        <  SRR4302533.6052055/94‑1 (MQ=60)

                                       GACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGG                                                       <  SRR4302533.5364227/94‑1 (MQ=60)

                                        ACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGG                                                      >  SRR4302533.3544372/1‑94 (MQ=60)

                                         CACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGA                                                     <  SRR4302533.2991575/94‑1 (MQ=60)

                                         CACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGA                                                     <  SRR4302533.6046308/94‑1 (MQ=60)

                                         CACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGA                                                     <  SRR4302533.386704/94‑1 (MQ=60)

                                         CACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGA                                                     >  SRR4302533.5351107/1‑94 (MQ=60)

                                         CACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGA                                                     >  SRR4302533.2498847/1‑94 (MQ=60)

                                         CACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGA                                                     >  SRR4302533.6116210/1‑94 (MQ=60)

                                          ACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAA                                                    <  SRR4302533.400103/94‑1 (MQ=60)

                                          ACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAA                                                    >  SRR4302533.571224/1‑94 (MQ=60)

                                          ACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAA                                                    >  SRR4302533.896772/1‑94 (MQ=60)

                                           CCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACACAGGGCGTGCACTGGGAAC                                                   >  SRR4302533.786153/1‑94 (MQ=60)

                                           CCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAAC                                                   >  SRR4302533.4911795/1‑94 (MQ=60)

                                           CCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAAC                                                   <  SRR4302533.4930082/94‑1 (MQ=60)

                                           CCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAAC                                                   >  SRR4302533.746492/1‑94 (MQ=60)

                                            CATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACC                                                  <  SRR4302533.4141470/94‑1 (MQ=60)

                                             ATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCA                                                 >  SRR4302533.3283362/1‑94 (MQ=60)

                                             ATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCA                                                 >  SRR4302533.5211920/1‑94 (MQ=60)

                                              TTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCAT                                                <  SRR4302533.71031/94‑1 (MQ=60)

                                               TATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATT                                               <  SRR4302533.3508040/94‑1 (MQ=60)

                                               TATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATT                                               >  SRR4302533.3039261/1‑94 (MQ=60)

                                               TATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATT                                               >  SRR4302533.3018038/1‑94 (MQ=60)

                                               TATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATT                                               <  SRR4302533.5788578/94‑1 (MQ=60)

                                               TATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATT                                               <  SRR4302533.3506552/94‑1 (MQ=60)

                                                 TGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGG                                             >  SRR4302533.1326081/1‑94 (MQ=60)

                                                 TGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGG                                             >  SRR4302533.3470727/1‑94 (MQ=60)

                                                 TGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGG                                             <  SRR4302533.4823684/94‑1 (MQ=60)

                                                  GGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGT                                            >  SRR4302533.2557995/1‑94 (MQ=60)

                                                   GCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTC                                           <  SRR4302533.1012099/94‑1 (MQ=60)

                                                    CGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCA                                          >  SRR4302533.2611538/1‑94 (MQ=60)

                                                    CGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCA                                          >  SRR4302533.127536/1‑94 (MQ=60)

                                                    CGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCA                                          >  SRR4302533.5938285/1‑94 (MQ=60)

                                                    CGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCA                                          >  SRR4302533.4329591/1‑94 (MQ=60)

                                                      TTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATA                                        <  SRR4302533.2845792/94‑1 (MQ=60)

                                                        GGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATT                                      <  SRR4302533.3164162/94‑1 (MQ=60)

                                                          CGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGTGCGTGCACTGGGAACCATTGGTCATATTGG                                    >  SRR4302533.6294807/1‑94 (MQ=60)

                                                           GAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGC                                   >  SRR4302533.2115958/1‑94 (MQ=60)

                                                           GAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGC                                   >  SRR4302533.4000765/1‑94 (MQ=60)

                                                           GAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGC                                   >  SRR4302533.1253478/1‑94 (MQ=60)

                                                           GAAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGC                                   >  SRR4302533.687102/1‑94 (MQ=60)

                                                            AAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCT                                  <  SRR4302533.4810839/94‑1 (MQ=60)

                                                            AAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCT                                  <  SRR4302533.5133016/94‑1 (MQ=60)

                                                            AAAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGAGTGCACTGGGAACCATTGGTCATATTGGCT                                  <  SRR4302533.5346861/94‑1 (MQ=60)

                                                             AAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTG                                 >  SRR4302533.1136047/1‑94 (MQ=60)

                                                             AAAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTG                                 >  SRR4302533.481282/1‑94 (MQ=60)

                                                              AAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGC                                >  SRR4302533.6025093/1‑94 (MQ=60)

                                                              AAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGC                                <  SRR4302533.5238068/94‑1 (MQ=60)

                                                              AAAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGC                                >  SRR4302533.5986436/1‑94 (MQ=60)

                                                               AAGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCT                               <  SRR4302533.85954/94‑1 (MQ=60)

                                                               AAGCATAAGTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCT                               <  SRR4302533.1944863/94‑1 (MQ=60)

                                                                AGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTT                              >  SRR4302533.5922872/1‑94 (MQ=60)

                                                                AGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTT                              >  SRR4302533.1730586/1‑94 (MQ=60)

                                                                AGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTT                              >  SRR4302533.1133990/1‑94 (MQ=60)

                                                                AGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTT                              >  SRR4302533.2972543/1‑94 (MQ=60)

                                                                AGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTT                              <  SRR4302533.1313117/94‑1 (MQ=60)

                                                                AGCATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGGGATGTCCCCTTCAAAAGGGCGTGCACTGGaaacccttggcctaattggttcttt                              >  SRR4302533.6226155/1‑69 (MQ=56)

                                                                  CATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTA                            <  SRR4302533.1306711/94‑1 (MQ=60)

                                                                  CATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTA                            <  SRR4302533.23737/94‑1 (MQ=60)

                                                                  CATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTA                            >  SRR4302533.3649212/1‑94 (MQ=60)

                                                                   ATAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAG                           <  SRR4302533.6020223/94‑1 (MQ=60)

                                                                    TAATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGC                          <  SRR4302533.766693/94‑1 (MQ=60)

                                                                     AATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCT                         >  SRR4302533.4819101/1‑94 (MQ=60)

                                                                     AATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCT                         <  SRR4302533.2322736/94‑1 (MQ=60)

                                                                     AATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCT                         >  SRR4302533.6051219/1‑94 (MQ=60)

                                                                     AATTTGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCT                         >  SRR4302533.3566945/1‑94 (MQ=60)

                                                                         TGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCA                     >  SRR4302533.3071418/1‑94 (MQ=60)

                                                                         TGTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCA                     <  SRR4302533.3179342/94‑1 (MQ=60)

                                                                          GTTTGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCAT                    <  SRR4302533.59660/94‑1 (MQ=60)

                                                                             TGTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAA                 <  SRR4302533.2658348/94‑1 (MQ=60)

                                                                              GTGGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAA                >  SRR4302533.672968/1‑94 (MQ=60)

                                                                                GGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACC              <  SRR4302533.17196/94‑1 (MQ=60)

                                                                                GGTAGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACC              <  SRR4302533.1797682/94‑1 (MQ=60)

                                                                                   AGAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAAACAAA           >  SRR4302533.5137256/1‑94 (MQ=60)

                                                                                    GAAGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAA          >  SRR4302533.1596025/1‑94 (MQ=60)

                                                                                      AGATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAAC        <  SRR4302533.1574950/94‑1 (MQ=60)

                                                                                       GATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACT       >  SRR4302533.2188584/1‑94 (MQ=60)

                                                                                       GATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACT       <  SRR4302533.1838092/94‑1 (MQ=60)

                                                                                       GATGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACT       >  SRR4302533.5283926/1‑94 (MQ=60)

                                                                                         TGCTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTAC     <  SRR4302533.4239710/94‑1 (MQ=60)

                                                                                           CTGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACAC   >  SRR4302533.5622975/1‑94 (MQ=60)

                                                                                            TGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACG  <  SRR4302533.2755088/94‑1 (MQ=60)

                                                                                            TGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACG  <  SRR4302533.1331393/94‑1 (MQ=60)

                                                                                            TGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACG  <  SRR4302533.4589837/94‑1 (MQ=60)

                                                                                            TGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACG  <  SRR4302533.5138155/94‑1 (MQ=60)

ATCGTGCCGGTCCATTACGCGGGTGTGGCCTGCGAAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATTTGTTTGTGGTAGAAGATGCCGCTCAGGGCGTGATGTCCACTTACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACG  >  NC_000913/3973124‑3973309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`