Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,004,812	T→C	R448R (CGT→CGC)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,004,812	0	T	C	99.6%	752.8 / ‑4.6	234	R448R (CGT→CGC)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (116/117); minor base A (0/1); total (116/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 242 total aligned reads displayed.

TCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCT  >  NC_000913/4004722‑4004901

tCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCAt                                                                                         >  2:2528971/1‑93 (MQ=255)

tCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGCGGTGCTAATAACCAGCGCAt                                                                                         >  2:859023/1‑93 (MQ=255)

tCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGGTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCAt                                                                                         >  1:1825213/1‑93 (MQ=255)

 cAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATc                                                                                        <  1:74630/93‑1 (MQ=255)

 cAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATc                                                                                        <  2:4557533/93‑1 (MQ=255)

   gATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCcg                                                                                      >  2:2259380/1‑93 (MQ=255)

     ttGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCcgcg                                                                                    >  1:1134889/1‑93 (MQ=255)

     ttGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCcgcg                                                                                    >  1:5678084/1‑93 (MQ=255)

     ttGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCACATCcgcg                                                                                    <  2:6155956/93‑1 (MQ=255)

      tGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGa                                                                                   <  2:584043/93‑1 (MQ=255)

      tGCCCTTTCCACCATTGGCCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGa                                                                                   <  2:1827929/93‑1 (MQ=255)

       gCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGAc                                                                                  <  1:61342/93‑1 (MQ=255)

        cccTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACt                                                                                 <  1:3222706/93‑1 (MQ=255)

         ccTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTa                                                                                >  1:519018/1‑93 (MQ=255)

         ccTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTa                                                                                <  2:5291436/93‑1 (MQ=255)

          cTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTAt                                                                               <  2:5644715/93‑1 (MQ=255)

          cTTTCCACCATTGGTCGTGTGAATCAGCGGCTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTAt                                                                               >  1:5782918/1‑93 (MQ=255)

           tttCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATACGCGACTATg                                                                              >  2:3073812/1‑93 (MQ=255)

            ttCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATgg                                                                             >  1:6215690/1‑93 (MQ=255)

             tCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGt                                                                            <  2:520977/93‑1 (MQ=255)

              ccaccaTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTc                                                                           >  1:3673566/1‑93 (MQ=255)

              ccaccaTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTc                                                                           >  1:2352357/1‑93 (MQ=255)

              ccaccaTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTc                                                                           <  1:1492850/93‑1 (MQ=255)

              ccaccaTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTc                                                                           <  2:3384712/93‑1 (MQ=255)

               caccaTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCa                                                                          >  2:1572688/1‑93 (MQ=255)

                accaTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCAt                                                                         <  1:726790/93‑1 (MQ=255)

                accaTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCAt                                                                         >  2:1781216/1‑93 (MQ=255)

                  caTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGa                                                                       <  1:1975440/93‑1 (MQ=255)

                   aTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGAc                                                                      >  1:5495568/1‑93 (MQ=255)

                   aTTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGAc                                                                      <  1:5069442/93‑1 (MQ=255)

                     tGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACaa                                                                    <  1:3998508/93‑1 (MQ=255)

                      ggTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACaaa                                                                   <  2:4022612/93‑1 (MQ=255)

                        tCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACt                                                                 <  2:1732574/93‑1 (MQ=255)

                          gtgtGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGa                                                               <  2:2623784/93‑1 (MQ=255)

                          gtgtGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGa                                                               <  1:853790/93‑1 (MQ=255)

                            gtgAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGaaa                                                             <  1:2550057/93‑1 (MQ=255)

                             tgAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAg                                                            <  2:3719841/93‑1 (MQ=255)

                              gAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGt                                                           >  2:3540808/1‑93 (MQ=255)

                              gAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGt                                                           >  2:2114309/1‑93 (MQ=255)

                                aTCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCt                                                         <  2:2373372/93‑1 (MQ=255)

                                 tCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTa                                                        <  1:3536929/93‑1 (MQ=255)

                                 tCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTa                                                        >  1:1415964/1‑93 (MQ=255)

                                 tCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTa                                                        <  1:5556812/93‑1 (MQ=255)

                                 tCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTa                                                        <  2:1676305/93‑1 (MQ=255)

                                    gCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATgg                                                     <  1:3464718/93‑1 (MQ=255)

                                        tttGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATg                                                 >  1:1190907/1‑93 (MQ=255)

                                           gggATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGc                                              >  2:5788524/1‑93 (MQ=255)

                                           gggATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGc                                              <  2:3361650/93‑1 (MQ=255)

                                            ggATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGcc                                             >  1:2880954/1‑93 (MQ=255)

                                            ggATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGcc                                             <  2:4883193/93‑1 (MQ=255)

                                                  gTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATa                                       >  1:3395212/1‑93 (MQ=255)

                                                  gTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATa                                       >  2:4450130/1‑93 (MQ=255)

                                                   ttATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATaa                                      >  2:5469532/1‑93 (MQ=255)

                                                    tATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATaaa                                     >  1:1667334/1‑93 (MQ=255)

                                                      tgtgGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAg                                   <  1:1767291/93‑1 (MQ=255)

                                                       gtggtgGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGc                                  <  2:2633775/93‑1 (MQ=255)

                                                       gtggtgGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGc                                  <  1:4868794/93‑1 (MQ=255)

                                                          gtggAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCAt                               <  1:1040223/93‑1 (MQ=255)

                                                           tggAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATg                              <  2:3247104/93‑1 (MQ=255)

                                                           tggAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATg                              >  2:2327904/1‑93 (MQ=255)

                                                             gAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGaa                            >  1:3631226/1‑93 (MQ=255)

                                                             gAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGaa                            <  2:2481855/93‑1 (MQ=255)

                                                              aaGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAAc                           >  1:1365114/1‑93 (MQ=255)

                                                                gTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACAt                         >  2:3833821/1‑93 (MQ=255)

                                                                gTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACAt                         >  1:121320/1‑93 (MQ=255)

                                                                  gATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTg                       >  2:4622974/1‑93 (MQ=255)

                                                                  gATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTg                       <  2:2076310/93‑1 (MQ=255)

                                                                  gATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTg                       >  1:5133370/1‑93 (MQ=255)

                                                                  gATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTg                       >  2:4835530/1‑93 (MQ=255)

                                                                  gATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTg                       <  2:4810074/93‑1 (MQ=255)

                                                                     tCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGt                    >  1:2910997/1‑93 (MQ=255)

                                                                      cGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTg                   <  2:1450895/93‑1 (MQ=255)

                                                                      cGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTg                   >  1:3750858/1‑93 (MQ=255)

                                                                       gtggtgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGa                  <  2:4215022/93‑1 (MQ=255)

                                                                       gtggtgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGa                  >  2:4258319/1‑93 (MQ=255)

                                                                       gtggtgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGa                  >  1:2654682/1‑93 (MQ=255)

                                                                       gtggtgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGa                  <  1:426018/93‑1 (MQ=255)

                                                                        tggtgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAg                 >  1:4315170/1‑93 (MQ=255)

                                                                        tggtgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAg                 >  2:2416106/1‑93 (MQ=255)

                                                                          gtgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCg               >  1:1058371/1‑93 (MQ=255)

                                                                           tgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGt              >  1:4593378/1‑93 (MQ=255)

                                                                           tgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGt              <  2:8888/93‑1 (MQ=255)

                                                                           tgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGt              <  2:1590839/93‑1 (MQ=255)

                                                                           tgCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGt              >  1:5323921/1‑93 (MQ=255)

                                                                            gCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTg             >  1:2702180/1‑93 (MQ=255)

                                                                             cTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGa            <  1:2259380/93‑1 (MQ=255)

                                                                             cTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGa            <  2:649910/93‑1 (MQ=255)

                                                                              taataaCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGAt           >  1:896443/1‑93 (MQ=255)

                                                                               aataaCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATc          >  2:4525320/1‑93 (MQ=255)

                                                                               aataaCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATc          >  2:507866/1‑93 (MQ=255)

                                                                               aataaCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATc          >  1:190442/1‑93 (MQ=255)

                                                                                ataaCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATcc         >  1:5930776/1‑93 (MQ=255)

                                                                                ataaCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATcc         >  1:4172560/1‑93 (MQ=255)

                                                                                  aaCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGc       <  1:296157/93‑1 (MQ=255)

                                                                                   aCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGcc      >  2:1153896/1‑93 (MQ=255)

                                                                                   aCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGcc      >  1:6041194/1‑93 (MQ=255)

                                                                                   aCCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGcc      <  1:1277214/93‑1 (MQ=255)

                                                                                      aGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGc   <  2:1339146/93‑1 (MQ=255)

                                                                                       gcgcATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCt  >  2:120518/1‑93 (MQ=255)

                                                                                       gcgcATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCt  <  1:2066960/93‑1 (MQ=255)

TCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCT  >  NC_000913/4004722‑4004901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATT  >  NC_000913/4004719‑4004905

TGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGC                                                                                               <  SRR4302533.878141/94‑1 (MQ=60)

  CTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGGTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCAT                                                                                             >  SRR4302533.1836363/1‑94 (MQ=60)

   TCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATC                                                                                            >  SRR4302533.3255912/1‑94 (MQ=60)

    CAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCC                                                                                           <  SRR4302533.4262411/94‑1 (MQ=60)

    CAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCC                                                                                           <  SRR4302533.75115/94‑1 (MQ=60)

     AGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCG                                                                                          >  SRR4302533.653746/1‑94 (MQ=60)

      GATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGC                                                                                         <  SRR4302533.2745945/94‑1 (MQ=60)

       ATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCG                                                                                        >  SRR4302533.1141638/1‑94 (MQ=60)

       ATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCG                                                                                        >  SRR4302533.5056982/1‑94 (MQ=60)

       ATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCG                                                                                        >  SRR4302533.5712377/1‑94 (MQ=60)

         TGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGAC                                                                                      >  SRR4302533.3666576/1‑94 (MQ=60)

          GCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACT                                                                                     <  SRR4302533.61745/94‑1 (MQ=60)

           CCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTA                                                                                    <  SRR4302533.3242581/94‑1 (MQ=60)

           CCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTA                                                                                    >  SRR4302533.522102/1‑94 (MQ=60)

           CCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTA                                                                                    >  SRR4302533.807186/1‑94 (MQ=60)

           CCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTA                                                                                    >  SRR4302533.117301/1‑94 (MQ=60)

            CCTTTCCACCATTGGTCGTGTGAATCAGCGGCTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTAT                                                                                   >  SRR4302533.5817845/1‑94 (MQ=60)

             CTTTCCGCCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATG                                                                                  <  SRR4302533.1490564/94‑1 (MQ=60)

             CTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATG                                                                                  <  SRR4302533.5121615/94‑1 (MQ=60)

             CTTTCCACCATTGGTCGTGTGAATCAGCGGCTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATG                                                                                  >  SRR4302533.5965534/1‑94 (MQ=60)

              TTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGG                                                                                 >  SRR4302533.6252960/1‑94 (MQ=60)

                TCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTC                                                                               >  SRR4302533.2366909/1‑94 (MQ=60)

                TCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTC                                                                               >  SRR4302533.3696141/1‑94 (MQ=60)

                 CCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCA                                                                              <  SRR4302533.1501853/94‑1 (MQ=60)

                  CACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCAT                                                                             <  SRR4302533.1183035/94‑1 (MQ=60)

                   ACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATG                                                                            <  SRR4302533.731083/94‑1 (MQ=60)

                   ACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATG                                                                            >  SRR4302533.2464456/1‑94 (MQ=60)

                    CCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGA                                                                           >  SRR4302533.5841586/1‑94 (MQ=60)

                    CCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGA                                                                           <  SRR4302533.3726551/94‑1 (MQ=60)

                    CCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGA                                                                           <  SRR4302533.4819550/94‑1 (MQ=60)

                     CATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGAC                                                                          >  SRR4302533.724572/1‑94 (MQ=60)

                     CATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGAC                                                                          <  SRR4302533.1367282/94‑1 (MQ=60)

                     CATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGAC                                                                          <  SRR4302533.1987537/94‑1 (MQ=60)

                     CATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGAC                                                                          >  SRR4302533.5528715/1‑94 (MQ=60)

                      ATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACA                                                                         <  SRR4302533.5100052/94‑1 (MQ=60)

                        TGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAA                                                                       <  SRR4302533.4022851/94‑1 (MQ=60)

                          GTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACT                                                                     >  SRR4302533.5694208/1‑94 (MQ=60)

                            CGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGA                                                                   >  SRR4302533.139140/1‑94 (MQ=60)

                            CGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGA                                                                   >  SRR4302533.1797876/1‑94 (MQ=60)

                            CGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGA                                                                   <  SRR4302533.1137704/94‑1 (MQ=60)

                             GTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAA                                                                  <  SRR4302533.858791/94‑1 (MQ=60)

                             GTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAA                                                                  <  SRR4302533.5540886/94‑1 (MQ=60)

                               GTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAG                                                                <  SRR4302533.2565753/94‑1 (MQ=60)

                                TGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGT                                                               <  SRR4302533.2800304/94‑1 (MQ=60)

                                 GAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTC                                                              >  SRR4302533.872872/1‑94 (MQ=60)

                                   ATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTA                                                            <  SRR4302533.3432826/94‑1 (MQ=60)

                                   ATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTA                                                            >  SRR4302533.1424531/1‑94 (MQ=60)

                                    TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTAT                                                           <  SRR4302533.3558728/94‑1 (MQ=60)

                                    TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTAT                                                           <  SRR4302533.5590394/94‑1 (MQ=60)

                                    TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTAT                                                           <  SRR4302533.1061624/94‑1 (MQ=60)

                                     CAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATG                                                          <  SRR4302533.355973/94‑1 (MQ=60)

                                     CAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATG                                                          <  SRR4302533.622761/94‑1 (MQ=60)

                                       GCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGC                                                        >  SRR4302533.4159064/1‑94 (MQ=60)

                                       GCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGC                                                        <  SRR4302533.1204648/94‑1 (MQ=60)

                                       GCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGC                                                        <  SRR4302533.3486104/94‑1 (MQ=60)

                                         GGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCAT                                                      >  SRR4302533.1643951/1‑94 (MQ=60)

                                          GTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATG                                                     <  SRR4302533.4161521/94‑1 (MQ=60)

                                          GTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATG                                                     <  SRR4302533.5759327/94‑1 (MQ=60)

                                          GTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATG                                                     >  SRR4302533.1198063/1‑94 (MQ=60)

                                           TTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGG                                                    <  SRR4302533.1842636/94‑1 (MQ=60)

                                           TTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGG                                                    <  SRR4302533.615621/94‑1 (MQ=60)

                                              GGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCC                                                 >  SRR4302533.2898679/1‑94 (MQ=60)

                                               GGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCG                                                <  SRR4302533.2686201/94‑1 (MQ=60)

                                               GGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCG                                                >  SRR4302533.4343481/1‑94 (MQ=60)

                                                 ATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTG                                              <  SRR4302533.19566/94‑1 (MQ=60)

                                                    GGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATA                                           >  SRR4302533.3416180/1‑94 (MQ=60)

                                                    GGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATA                                           >  SRR4302533.2261994/1‑94 (MQ=60)

                                                      TTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAA                                         >  SRR4302533.3598697/1‑94 (MQ=60)

                                                      TTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAA                                         >  SRR4302533.1677406/1‑94 (MQ=60)

                                                       TATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAA                                        >  SRR4302533.2918797/1‑94 (MQ=60)

                                                       TATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAA                                        >  SRR4302533.554558/1‑94 (MQ=60)

                                                       TATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAA                                        <  SRR4302533.2969048/94‑1 (MQ=60)

                                                        ATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAG                                       >  SRR4302533.1706283/1‑94 (MQ=60)

                                                        ATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAG                                       <  SRR4302533.5053183/94‑1 (MQ=60)

                                                         TGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGC                                      >  SRR4302533.5831/1‑94 (MQ=60)

                                                         TGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGC                                      <  SRR4302533.1778051/94‑1 (MQ=60)

                                                          GTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCC                                     <  SRR4302533.4898295/94‑1 (MQ=60)

                                                           TGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCA                                    <  SRR4302533.3767921/94‑1 (MQ=60)

                                                           TGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCA                                    <  SRR4302533.3065207/94‑1 (MQ=60)

                                                             GTGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATG                                  <  SRR4302533.1046339/94‑1 (MQ=60)

                                                              TGGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGA                                 >  SRR4302533.5172389/1‑94 (MQ=60)

                                                               GGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAA                                >  SRR4302533.6081874/1‑94 (MQ=60)

                                                               GGAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAA                                >  SRR4302533.3653566/1‑94 (MQ=60)

                                                                GAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAAC                               >  SRR4302533.1373321/1‑94 (MQ=60)

                                                                GAAGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAAC                               <  SRR4302533.3319205/94‑1 (MQ=60)

                                                                  AGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACAT                             <  SRR4302533.2206551/94‑1 (MQ=60)

                                                                  AGTGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACAT                             >  SRR4302533.122086/1‑94 (MQ=60)

                                                                    TGATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTG                           >  SRR4302533.5164380/1‑94 (MQ=60)

                                                                     GATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGG                          >  SRR4302533.1470111/1‑94 (MQ=60)

                                                                      ATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGG                         <  SRR4302533.5354706/94‑1 (MQ=60)

                                                                      ATTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGG                         <  SRR4302533.1766329/94‑1 (MQ=60)

                                                                       TTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGT                        >  SRR4302533.2572475/1‑94 (MQ=60)

                                                                       TTCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGT                        >  SRR4302533.2928867/1‑94 (MQ=60)

                                                                        TCGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTG                       >  SRR4302533.3773855/1‑94 (MQ=60)

                                                                         CGTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGA                      >  SRR4302533.2670988/1‑94 (MQ=60)

                                                                          GTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAG                     >  SRR4302533.4341152/1‑94 (MQ=60)

                                                                          GTGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAG                     <  SRR4302533.428560/94‑1 (MQ=60)

                                                                           TGGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGC                    >  SRR4302533.3788118/1‑94 (MQ=60)

                                                                            GGTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCG                   >  SRR4302533.1064596/1‑94 (MQ=60)

                                                                             GTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGT                  >  SRR4302533.4621203/1‑94 (MQ=60)

                                                                             GTGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGT                  >  SRR4302533.5355827/1‑94 (MQ=60)

                                                                              TGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTG                 >  SRR4302533.2718756/1‑94 (MQ=60)

                                                                              TGCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTG                 <  SRR4302533.1267943/94‑1 (MQ=60)

                                                                               GCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGA                >  SRR4302533.769466/1‑94 (MQ=60)

                                                                               GCTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGA                <  SRR4302533.2070560/94‑1 (MQ=60)

                                                                                CTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGAT               >  SRR4302533.35191/1‑94 (MQ=60)

                                                                                CTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGAT               <  SRR4302533.2273357/94‑1 (MQ=60)

                                                                                CTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGAT               <  SRR4302533.2282532/94‑1 (MQ=60)

                                                                                CTAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGAT               >  SRR4302533.901706/1‑94 (MQ=60)

                                                                                 TAATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATC              >  SRR4302533.191636/1‑94 (MQ=60)

                                                                                  AATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGTATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCC             <  SRR4302533.4458616/94‑1 (MQ=60)

                                                                                  AATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCC             >  SRR4302533.5966631/1‑94 (MQ=60)

                                                                                  AATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCC             >  SRR4302533.4197843/1‑94 (MQ=60)

                                                                                  AATAACCAGCGAATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCC             <  SRR4302533.5489216/94‑1 (MQ=60)

                                                                                   ATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCG            >  SRR4302533.2960509/1‑94 (MQ=60)

                                                                                   ATAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCG            >  SRR4302533.1266357/1‑94 (MQ=60)

                                                                                    TAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGC           >  SRR4302533.4330648/1‑94 (MQ=60)

                                                                                    TAACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGC           <  SRR4302533.1897499/94‑1 (MQ=60)

                                                                                     AACCAGCGCATCCGCGACTGTGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCC          >  SRR4302533.6077801/1‑94 (MQ=60)

                                                                                     AACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCC          >  SRR4302533.6077802/1‑94 (MQ=60)

                                                                                     AACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCC          <  SRR4302533.297969/94‑1 (MQ=60)

                                                                                     AACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCC          >  SRR4302533.741409/1‑94 (MQ=60)

                                                                                      ACCAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCA         <  SRR4302533.1284890/94‑1 (MQ=60)

                                                                                        CAGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGC       >  SRR4302533.5294376/1‑94 (MQ=60)

                                                                                         AGCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCT      <  SRR4302533.2525247/94‑1 (MQ=60)

                                                                                          GCGCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTG     <  SRR4302533.2079696/94‑1 (MQ=60)

                                                                                            GCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGAT   >  SRR4302533.1261377/1‑94 (MQ=60)

                                                                                            GCATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGAT   <  SRR4302533.4750184/94‑1 (MQ=60)

                                                                                             CATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATT  >  SRR4302533.1385706/1‑94 (MQ=60)

TGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATT  >  NC_000913/4004719‑4004905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`