Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,007,907 +GG intergenic (+109/‑11) pldB → / → b4555 lysophospholipase L(2)/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,007,9071.G93.1% 706.8 / 44.3 233intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (11/5);  new base G (127/90);  total (138/95)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
*NC_0009134,007,9072.G93.1% 700.2 / 44.3 233intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (11/5);  new base G (127/90);  total (138/95)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 262 total aligned reads displayed.

GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996
                                                                                             ||                                                                                         
  tgttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgcgg                                                                                           <  1:4058073/93‑3 (MQ=255)
   gttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgcggc                                                                                          >  2:3054107/1‑90 (MQ=255)
    ttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgcggca                                                                                         >  1:5032559/1‑89 (MQ=255)
    ttgCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCgcgcgcggca                                                                                         >  1:482936/1‑89 (MQ=255)
           tGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACtt                                                                                  >  1:4632556/1‑93 (MQ=255)
              tttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTtgt                                                                               >  1:2688208/1‑93 (MQ=255)
               ttAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTtgtg                                                                              <  2:4430055/93‑1 (MQ=255)
                tAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTgtgt                                                                             <  2:6294372/93‑1 (MQ=255)
                 aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGtt                                                                            >  1:6198716/1‑93 (MQ=255)
                 aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGtt                                                                            >  1:4737029/1‑93 (MQ=255)
                  gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGttt                                                                           <  2:5763209/93‑1 (MQ=255)
                   aTGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTg                                                                          <  1:3413880/93‑1 (MQ=255)
                    tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGc                                                                         >  2:2784899/1‑93 (MQ=255)
                    tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGc                                                                         >  1:364682/1‑93 (MQ=255)
                      gCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGa                                                                       <  1:5712911/93‑1 (MQ=255)
                       cACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGAc                                                                      <  1:5410841/93‑1 (MQ=255)
                        aCGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAACCTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGAcc                                                                     <  1:635265/93‑1 (MQ=255)
                        aCGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGAcc                                                                     >  1:4535160/1‑93 (MQ=255)
                         cGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCg                                                                    >  2:677286/1‑93 (MQ=255)
                         cGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCg                                                                    >  2:3422515/1‑93 (MQ=255)
                          gTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                                   >  2:1104608/1‑93 (MQ=255)
                          gTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                                   >  1:2008611/1‑93 (MQ=255)
                          cttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                                   <  2:3130064/92‑1 (MQ=255)
                           ttACTTTCGCCAGACCATACGTTATCCCCTTACGCTAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                                  <  2:591711/93‑1 (MQ=255)
                            tACTTTCTCCCGACCATACGTTATCCCCTTACGCCCAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtc                                                                 >  1:5542905/1‑93 (MQ=255)
                            tACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtc                                                                 >  1:2857822/1‑93 (MQ=255)
                            tACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtc                                                                 <  1:20776/93‑1 (MQ=255)
                            tACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtc                                                                 <  2:5132671/93‑1 (MQ=255)
                            tACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtc                                                                 <  2:3666903/93‑1 (MQ=255)
                              cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                                               >  2:5024954/1‑93 (MQ=255)
                              cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                                               >  2:2562249/1‑93 (MQ=255)
                              cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                                               >  1:2635358/1‑93 (MQ=255)
                              cTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                                               >  2:4487462/1‑93 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              >  2:5645649/1‑93 (MQ=255)
                               tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                                              >  2:409704/1‑93 (MQ=255)
                                  ctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacc                                                           >  2:5437108/1‑93 (MQ=255)
                                  ctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacc                                                           >  1:778050/1‑93 (MQ=255)
                                   tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacca                                                          >  1:5796320/1‑93 (MQ=255)
                                   tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacca                                                          <  1:6077567/93‑1 (MQ=255)
                                     ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg                                                        >  1:5593641/1‑93 (MQ=255)
                                     ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg                                                        >  1:1733574/1‑93 (MQ=255)
                                      cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGt                                                       <  1:901793/93‑1 (MQ=255)
                                       gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGtt                                                      <  2:786277/93‑1 (MQ=255)
                                       gACCATACGTCATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGtt                                                      <  1:1059865/93‑1 (MQ=255)
                                        aCCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTg                                                     >  2:1924312/1‑93 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    <  2:3778312/93‑1 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    <  2:4244927/93‑1 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    >  2:1190578/1‑93 (MQ=255)
                                         ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGa                                                    <  2:1368532/93‑1 (MQ=255)
                                           aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg                                                  >  1:1471711/1‑93 (MQ=255)
                                           aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg                                                  <  2:2927569/93‑1 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 >  2:4731369/1‑93 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 <  2:3100449/93‑1 (MQ=255)
                                            tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 >  1:2170162/1‑93 (MQ=255)
                                            tACGTTATCCCCTCACGCCAAAGAAGCTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                                                 <  2:1215307/93‑1 (MQ=255)
                                             aCGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                                                <  1:1328890/93‑1 (MQ=255)
                                              cGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgg                                               <  2:5695459/93‑1 (MQ=255)
                                               gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                                              <  1:1167361/93‑1 (MQ=255)
                                                ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg                                             >  1:3283540/1‑93 (MQ=255)
                                                ttATCCCCTCACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgggg                                             <  1:4905686/93‑1 (MQ=255)
                                                 tATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGc                                            >  2:3918923/1‑93 (MQ=255)
                                                  aTCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCa                                           <  2:2488464/93‑1 (MQ=255)
                                                  aTCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCa                                           >  1:4327207/1‑93 (MQ=255)
                                                   tCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGTGGCaa                                          >  1:533247/1‑93 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         >  2:2444517/1‑93 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         <  2:2326040/93‑1 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         >  2:4938994/1‑93 (MQ=255)
                                                    ccccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         <  2:158269/93‑1 (MQ=255)
                                                    ccccTGACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaaa                                         <  1:3187617/93‑1 (MQ=255)
                                                     cccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCCTCACCACGTTGATGTGGGGCAAAt                                        <  2:6073239/93‑1 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       >  1:1618616/1‑93 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       >  1:60000/1‑93 (MQ=255)
                                                      ccTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAtt                                       <  2:323329/93‑1 (MQ=255)
                                                        ttACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcg                                     >  2:2055826/1‑93 (MQ=255)
                                                         tACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc                                    <  2:2714892/93‑1 (MQ=255)
                                                         tACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc                                    >  1:3725884/1‑93 (MQ=255)
                                                          aCGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgcg                                   <  1:5911993/93‑1 (MQ=255)
                                                          aCGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgcg                                   >  2:509635/1‑93 (MQ=255)
                                                            gCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt                                 >  2:327085/1‑93 (MQ=255)
                                                            gCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt                                 <  2:4134594/93‑1 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                >  1:1024277/1‑93 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                >  1:6148610/1‑93 (MQ=255)
                                                             ccAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa                                >  2:5375502/1‑93 (MQ=255)
                                                              cAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATaa                               >  1:3545063/1‑93 (MQ=255)
                                                                aaGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATc                             >  2:756784/1‑93 (MQ=255)
                                                                aaGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATc                             >  1:165971/1‑93 (MQ=255)
                                                                 aGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCt                            >  1:3927889/1‑93 (MQ=255)
                                                                 aGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCt                            <  1:2937751/93‑1 (MQ=255)
                                                                 aGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGCGGCATATTCGCGATAATCt                            >  1:5799501/1‑93 (MQ=255)
                                                                       tctGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt                      >  2:4610444/1‑93 (MQ=255)
                                                                        ctGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAtt                     <  2:6128656/93‑1 (MQ=255)
                                                                         tGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa                    >  2:169671/1‑93 (MQ=255)
                                                                           aaGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAg                  <  1:1786414/93‑1 (MQ=255)
                                                                            aGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGt                 >  2:5340361/1‑93 (MQ=255)
                                                                             gctgctCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTc                <  2:452118/93‑1 (MQ=255)
                                                                                gctCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTa             <  2:616159/93‑1 (MQ=255)
                                                                                  cccgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATg         <  2:4396369/89‑1 (MQ=255)
                                                                                   ccgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGa        >  1:272869/4‑93 (MQ=255)
                                                                                   ccgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGa        >  1:539528/4‑93 (MQ=255)
                                                                                   ccgcgcgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGa        >  1:2404554/4‑93 (MQ=255)
                                                                                             ||                                                                                         
GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT  >  NC_000913/4007815‑4007996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000
                                                                                             |                                                                                               
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC                                                                                                 >  SRR4302533.5993037/1‑94 (MQ=60)
 GTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑G                                                                                              >  SRR4302533.4435814/1‑94 (MQ=60)
  TTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCgg                                                                                               <  SRR4302533.5077768/94‑3 (MQ=60)
   TGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggc                                                                                              <  SRR4302533.4082777/94‑4 (MQ=60)
    GTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggca                                                                                             >  SRR4302533.5063047/1‑90 (MQ=60)
    GTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggca                                                                                             <  SRR4302533.3352717/94‑5 (MQ=60)
    GTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggca                                                                                             >  SRR4302533.485818/1‑90 (MQ=60)
    GTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggca                                                                                             <  SRR4302533.6019565/94‑5 (MQ=60)
     TTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCggcat                                                                                            >  SRR4302533.4109058/1‑89 (MQ=60)
        CGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAA                                                                                         >  SRR4302533.2772525/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAAC                                                                                        >  SRR4302533.2414277/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAAC                                                                                        >  SRR4302533.4299780/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAAC                                                                                        >  SRR4302533.2828886/1‑94 (MQ=60)
         GTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAAC                                                                                        <  SRR4302533.4145036/94‑1 (MQ=60)
           CTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTT                                                                                      >  SRR4302533.4660630/1‑94 (MQ=60)
           CTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTT                                                                                      >  SRR4302533.3725510/1‑94 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTT                                                                                     >  SRR4302533.4172084/1‑94 (MQ=60)
            TGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTT                                                                                     >  SRR4302533.2044219/1‑94 (MQ=60)
              ATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGT                                                                                   >  SRR4302533.2704689/1‑94 (MQ=60)
               TTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTG                                                                                  <  SRR4302533.6182415/94‑1 (MQ=60)
                 TAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTT                                                                                >  SRR4302533.6235901/1‑94 (MQ=60)
                 TAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTT                                                                                >  SRR4302533.4765752/1‑94 (MQ=60)
                 TAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTT                                                                                >  SRR4302533.5396089/1‑94 (MQ=60)
                   GATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTG                                                                              <  SRR4302533.1737068/94‑1 (MQ=60)
                   GATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTG                                                                              >  SRR4302533.5204546/1‑94 (MQ=60)
                   GATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTG                                                                              <  SRR4302533.5927849/94‑1 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGC                                                                             <  SRR4302533.3434973/94‑1 (MQ=60)
                    ATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGC                                                                             >  SRR4302533.366895/1‑94 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            <  SRR4302533.5814447/94‑1 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            <  SRR4302533.2023109/94‑1 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            >  SRR4302533.2837846/1‑94 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            <  SRR4302533.3223757/94‑1 (MQ=60)
                     TGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCG                                                                            <  SRR4302533.1435539/94‑1 (MQ=60)
                      GGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGA                                                                           <  SRR4302533.4635048/94‑1 (MQ=60)
                       GCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGAC                                                                          >  SRR4302533.4728875/1‑94 (MQ=60)
                       GCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGAC                                                                          <  SRR4302533.5747420/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         <  SRR4302533.1773508/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         <  SRR4302533.634684/94‑1 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302533.4562620/1‑94 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         >  SRR4302533.4655925/1‑94 (MQ=60)
                        CACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACC                                                                         <  SRR4302533.5443372/94‑1 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAACCTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        <  SRR4302533.639001/94‑1 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        >  SRR4302533.763822/1‑94 (MQ=60)
                         ACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCG                                                                        >  SRR4302533.2326000/1‑94 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       >  SRR4302533.2020981/1‑94 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       >  SRR4302533.5705948/1‑94 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       <  SRR4302533.6056800/94‑1 (MQ=60)
                          CGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGG                                                                       >  SRR4302533.214678/1‑94 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCCAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     >  SRR4302533.5576380/1‑94 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     >  SRR4302533.2875404/1‑94 (MQ=60)
                            TTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTC                                                                     <  SRR4302533.1879996/94‑1 (MQ=60)
                             TACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCG                                                                    <  SRR4302533.20913/94‑1 (MQ=60)
                              ACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGT                                                                   >  SRR4302533.2732908/1‑94 (MQ=60)
                              ACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGT                                                                   >  SRR4302533.2651554/1‑94 (MQ=60)
                               CTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTC                                                                  >  SRR4302533.3003573/1‑94 (MQ=60)
                                  TCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACC                                                               >  SRR4302533.782629/1‑94 (MQ=60)
                                   CTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCA                                                              >  SRR4302533.5831341/1‑94 (MQ=60)
                                    TCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCAC                                                             <  SRR4302533.6267091/94‑1 (MQ=60)
                                    TCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCAC                                                             <  SRR4302533.6114325/94‑1 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            >  SRR4302533.1744092/1‑94 (MQ=60)
                                     CCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACG                                                            >  SRR4302533.5627435/1‑94 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          >  SRR4302533.4286314/1‑94 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          <  SRR4302533.907082/94‑1 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          <  SRR4302533.2945649/94‑1 (MQ=60)
                                       CGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTT                                                          >  SRR4302533.291481/1‑94 (MQ=60)
                                        GACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         >  SRR4302533.3952857/1‑94 (MQ=60)
                                        GACCATACGTCATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTG                                                         <  SRR4302533.1066096/94‑1 (MQ=60)
                                         ACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGA                                                        >  SRR4302533.1110150/1‑94 (MQ=60)
                                          CCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAT                                                       >  SRR4302533.2557906/1‑94 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      <  SRR4302533.5832757/94‑1 (MQ=60)
                                           CATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATG                                                      >  SRR4302533.1480606/1‑94 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     >  SRR4302533.5898400/1‑94 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     >  SRR4302533.2183508/1‑94 (MQ=60)
                                            ATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGT                                                     >  SRR4302533.2731427/1‑94 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302533.1336865/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   <  SRR4302533.6202463/94‑1 (MQ=60)
                                              ACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGG                                                   >  SRR4302533.6121488/1‑94 (MQ=60)
                                               CGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGG                                                  >  SRR4302533.1614771/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 <  SRR4302533.1174365/94‑1 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302533.5748656/1‑94 (MQ=60)
                                                GTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGG                                                 >  SRR4302533.3303810/1‑94 (MQ=60)
                                                 TTATCCCCTCACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGC                                                <  SRR4302533.4935410/94‑1 (MQ=60)
                                                  TATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCA                                               >  SRR4302533.4353274/1‑94 (MQ=60)
                                                  TATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCA                                               >  SRR4302533.686331/1‑94 (MQ=60)
                                                   ATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGTGGCAA                                              >  SRR4302533.536418/1‑94 (MQ=60)
                                                   ATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAA                                              >  SRR4302533.2659779/1‑94 (MQ=60)
                                                   ATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAA                                              >  SRR4302533.146229/1‑94 (MQ=60)
                                                    TCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAA                                             <  SRR4302533.2651092/94‑1 (MQ=60)
                                                     CCCCTGACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAAT                                            <  SRR4302533.3207226/94‑1 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           >  SRR4302533.1628421/1‑94 (MQ=60)
                                                      CCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATT                                           >  SRR4302533.60391/1‑94 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCACATTCG                                         >  SRR4302533.3910269/1‑94 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         <  SRR4302533.3980862/94‑1 (MQ=60)
                                                        CTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCG                                         >  SRR4302533.5479907/1‑94 (MQ=60)
                                                         TTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGC                                        >  SRR4302533.3748742/1‑94 (MQ=60)
                                                           ACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGA                                      <  SRR4302533.3667261/94‑1 (MQ=60)
                                                           ACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGA                                      >  SRR4302533.5558328/1‑94 (MQ=60)
                                                           ACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGA                                      <  SRR4302533.5947718/94‑1 (MQ=60)
                                                            CGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGCGCAAATTCGCGAT                                     >  SRR4302533.3019253/1‑94 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATA                                    >  SRR4302533.6185624/1‑94 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATA                                    <  SRR4302533.1059460/94‑1 (MQ=60)
                                                             GCCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATA                                    >  SRR4302533.1030299/1‑94 (MQ=60)
                                                              CCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAA                                   >  SRR4302533.3566913/1‑94 (MQ=60)
                                                              CCAAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCCTCACCACGTTGATGTGGGGCAAATTCGCGCTAA                                   >  SRR4302533.1536333/1‑94 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 <  SRR4302533.4923023/94‑1 (MQ=60)
                                                                AAAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATC                                 >  SRR4302533.167024/1‑94 (MQ=60)
                                                                 AAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCT                                >  SRR4302533.3951869/1‑94 (MQ=60)
                                                                 AAGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGCGGCATATTCGCGATAATCT                                >  SRR4302533.5834539/1‑94 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTG                               >  SRR4302533.5368505/1‑94 (MQ=60)
                                                                  AGAAACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTG                               <  SRR4302533.2955705/94‑1 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            >  SRR4302533.4795759/1‑94 (MQ=60)
                                                                     AACTCTGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAG                            <  SRR4302533.369520/94‑1 (MQ=60)
                                                                          TGAAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAA                       >  SRR4302533.1104131/1‑94 (MQ=60)
                                                                            AAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     >  SRR4302533.5641868/1‑94 (MQ=60)
                                                                            AAGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGT                     <  SRR4302533.1797274/94‑1 (MQ=60)
                                                                             AGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302533.3519823/1‑94 (MQ=60)
                                                                             AGCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTC                    >  SRR4302533.1722946/1‑94 (MQ=60)
                                                                              GCTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCT                   <  SRR4302533.5580487/94‑1 (MQ=60)
                                                                               CTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCACATTCGCGATAATCTGGAGATTAAGTCTT                  >  SRR4302533.1830122/1‑94 (MQ=60)
                                                                               CTGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTT                  >  SRR4302533.818563/1‑94 (MQ=60)
                                                                                TGCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTA                 >  SRR4302533.5434198/1‑94 (MQ=60)
                                                                                 GCTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTAC                <  SRR4302533.350646/94‑1 (MQ=60)
                                                                                  CTCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACA               >  SRR4302533.4136247/1‑94 (MQ=60)
                                                                                   TCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              <  SRR4302533.4286872/94‑1 (MQ=60)
                                                                                   TCACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACAT              <  SRR4302533.933636/94‑1 (MQ=60)
                                                                                    CACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             >  SRR4302533.1266949/1‑94 (MQ=60)
                                                                                    CACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATG             >  SRR4302533.4923409/1‑94 (MQ=60)
                                                                                     ACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGA            >  SRR4302533.542735/1‑94 (MQ=60)
                                                                                     ACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGA            >  SRR4302533.2419404/1‑94 (MQ=60)
                                                                                     ACCGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGA            >  SRR4302533.274530/1‑94 (MQ=60)
                                                                                      GCGCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTA         <  SRR4302533.4852131/94‑1 (MQ=60)
                                                                                       CGCGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATT          >  SRR4302533.2220725/1‑94 (MQ=60)
                                                                                        GCGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACC       <  SRR4302533.914355/94‑1 (MQ=60)
                                                                                         CGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT      >  SRR4302533.3317268/1‑94 (MQ=60)
                                                                                         CGCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCT      <  SRR4302533.4941856/94‑1 (MQ=60)
                                                                                          GCGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTC     >  SRR4302533.5862585/1‑94 (MQ=60)
                                                                                           CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCC    >  SRR4302533.2570502/1‑94 (MQ=60)
                                                                                           CGG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCC    <  SRR4302533.933418/94‑1 (MQ=60)
                                                                                            GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   <  SRR4302533.3235173/94‑1 (MQ=60)
                                                                                            GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   <  SRR4302533.1635153/94‑1 (MQ=60)
                                                                                            GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   >  SRR4302533.592853/1‑94 (MQ=60)
                                                                                            GG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCA   >  SRR4302533.434857/1‑94 (MQ=60)
                                                                                             G‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  <  SRR4302533.920020/94‑1 (MQ=60)
                                                                                             |                                                                                               
GGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACCGCGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTAAGTCTTACATGATTACCTCCAA  >  NC_000913/4007814‑4008000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑