Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,429 +TG coding (512/516 nt) b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4291.T100.0% 412.6 / NA 110L171? (TTG→TNG) b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (52/58);  total (52/58)
*NC_0009134,008,4292.G100.0% 355.1 / NA 110L171W (TTG→TGG) b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (52/58);  total (52/58)

Only 100 of 123 total aligned reads displayed.

TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AA‑‑‑‑‑CT‑GGCTACAG‑CTGAAGGATTG‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCT  >  NC_000913/4008346‑4008503
                                                                                                    ||                                                                              
 cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑ttt                                                                                 >  2:2607369/1‑92 (MQ=255)
 cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑ttt                                                                                 <  1:3423220/93‑2 (MQ=255)
  tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑tttg                                                                                <  1:1051706/93‑3 (MQ=255)
  tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑tttg                                                                                >  1:5951006/1‑91 (MQ=255)
  tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑tttg                                                                                >  2:1156237/1‑91 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑tttgg                                                                               >  2:630739/1‑90 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGa                                                                       <  2:5970655/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGa                                                                       <  1:100493/93‑1 (MQ=255)
            ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑AAGAAACCG‑GGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGAt                                                                      <  2:1686766/93‑1 (MQ=255)
             gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATg                                                                     <  2:2236559/93‑1 (MQ=255)
             gcgggcggCGTGTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATg                                                                     <  2:790947/93‑1 (MQ=255)
              cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGa                                                                    <  1:5478423/93‑1 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGaa                                                                   <  1:2635131/93‑1 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGaa                                                                   <  2:3445771/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAac                                                                  <  1:4957056/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAac                                                                  >  1:3993000/1‑93 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAac                                                                  <  2:2772791/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAac                                                                  <  2:331868/93‑1 (MQ=255)
                 gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacg                                                                 <  1:2957114/93‑1 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacga                                                                <  2:2166732/93‑1 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacga                                                                <  1:2546223/93‑1 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTT‑‑GTGACGGGATGAacgac                                                               >  2:5773184/1‑93 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacgac                                                               >  2:3119511/1‑93 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacgac                                                               <  2:4279944/93‑1 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacgac                                                               <  2:4498313/93‑1 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacgac                                                               >  2:5281397/1‑93 (MQ=255)
                    gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAacgacg                                                              >  2:4300379/1‑93 (MQ=255)
                     cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGc                                                             >  2:5938791/1‑93 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGcc                                                            <  2:4808827/93‑1 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGcc                                                            <  1:4418546/93‑1 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGa                                                          >  2:5932837/5‑93 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGa                                                          <  2:2580522/89‑1 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGa                                                          >  1:6270373/5‑93 (MQ=255)
                         tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGaa                                                         >  2:476989/4‑93 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGaaa                                                        <  2:723500/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGaaa                                                        <  1:995345/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGaaa                                                        <  1:3434171/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGaaa                                                        >  1:4825533/3‑93 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGaaa                                                        >  1:4825534/3‑93 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAt                                                       >  2:3678743/2‑93 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAt                                                       <  2:2946514/92‑1 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAt                                                       >  1:5825289/2‑93 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAt                                                       >  2:4130459/2‑93 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGCAATg                                                      >  2:2085646/1‑93 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATg                                                      >  1:290367/1‑93 (MQ=255)
                             aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGc                                                     <  1:6192030/93‑1 (MQ=255)
                             aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGc                                                     >  2:3848605/1‑93 (MQ=255)
                             aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGc                                                     <  1:2935716/93‑1 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCt                                                    <  1:1197056/93‑1 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCt                                                    <  1:3735094/93‑1 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCt                                                    <  2:193059/93‑1 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCt                                                    >  2:6045723/1‑93 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                   <  1:241840/93‑1 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                   <  2:2269624/93‑1 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                   <  1:5385678/93‑1 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                   <  1:4092752/93‑1 (MQ=255)
                                                                 actgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATggg                    >  2:5517684/5‑93 (MQ=255)
                                                                 actgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATggg                    >  1:2688598/5‑93 (MQ=255)
                                                                 actgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAA‑GTCTGCATTATggg                    >  1:3118034/5‑93 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGc                   <  2:1450881/90‑1 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGc                   <  1:844949/90‑1 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGc                   >  2:2214314/4‑93 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGc                   >  1:4618390/4‑93 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGc                   <  1:4035461/90‑1 (MQ=255)
                                                                   tgggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCa                  >  1:1750273/3‑93 (MQ=255)
                                                                    gggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAg                 >  2:2095688/2‑93 (MQ=255)
                                                                    gggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAg                 <  2:5597564/92‑1 (MQ=255)
                                                                     ggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGt                <  2:236929/93‑1 (MQ=255)
                                                                     ggCTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGt                >  1:1525946/1‑93 (MQ=255)
                                                                       cTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTgc              <  2:2350087/93‑2 (MQ=255)
                                                                       cTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTgc              >  1:6162543/1‑92 (MQ=255)
                                                                       cTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTgc              <  2:6200703/93‑2 (MQ=255)
                                                                       cTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTgc              <  2:3528898/93‑2 (MQ=255)
                                                                       cTACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTgc              <  1:5539427/93‑2 (MQ=255)
                                                                        tACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTgcg             >  2:2417089/1‑91 (MQ=255)
                                                                        tACAGCCTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTgcg             >  1:5748970/1‑91 (MQ=255)
                                                                            gccTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCgcacc         >  1:1019375/3‑93 (MQ=255)
                                                                            gccTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCgcacc         >  2:3680671/3‑93 (MQ=255)
                                                                            gccTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCgcacc         >  1:5659547/3‑93 (MQ=255)
                                                                             ccTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCgcacca        >  2:5429639/2‑93 (MQ=255)
                                                                             ccTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCgcacca        >  1:97290/2‑93 (MQ=255)
                                                                              cTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAg       <  1:5857761/93‑1 (MQ=255)
                                                                              cTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAg       <  1:5209391/93‑1 (MQ=255)
                                                                              cTGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAg       >  1:2048371/1‑93 (MQ=255)
                                                                               tGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAATG‑CTGCATTATGGGCAGTGCGCACCAGc      >  1:2632995/1‑93 (MQ=255)
                                                                               tGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc      >  2:351796/1‑93 (MQ=255)
                                                                               tGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc      >  1:6103955/1‑93 (MQ=255)
                                                                               tGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc      >  1:1680921/1‑93 (MQ=255)
                                                                               tGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc      <  1:1744278/93‑1 (MQ=255)
                                                                               tGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc      >  2:3305289/1‑93 (MQ=255)
                                                                               tGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc      <  2:3465136/93‑1 (MQ=255)
                                                                                gAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGGAAG‑G‑CTGCATTATGGGCAGGGCGCACCACCg     >  2:5814954/1‑93 (MQ=255)
                                                                                gAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg     <  1:4711253/93‑1 (MQ=255)
                                                                                gAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg     <  2:5172308/93‑1 (MQ=255)
                                                                                gAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg     >  2:2994525/1‑93 (MQ=255)
                                                                                gAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg     >  1:310058/1‑93 (MQ=255)
                                                                                 aaGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGt    <  1:4623016/93‑1 (MQ=255)
                                                                                  aGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc   >  1:4773736/1‑93 (MQ=255)
                                                                                  aGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTc   >  1:1787620/1‑93 (MQ=255)
                                                                                   ggATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt  <  1:5903364/93‑1 (MQ=255)
                                                                                                    ||                                                                              
TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AA‑‑‑‑‑CT‑GGCTACAG‑CTGAAGGATTG‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCT  >  NC_000913/4008346‑4008503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCC  >  NC_000913/4008370‑4008519
                                                                   |                                                                                                
tcaAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAA                                                                        >  SRR4302533.4854776/4‑94 (MQ=16)
tcaAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAA                                                                        >  SRR4302533.4854775/4‑94 (MQ=16)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                       >  SRR4302533.5860484/3‑94 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                       <  SRR4302533.5801257/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                       <  SRR4302533.3455385/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                       <  SRR4302533.4581160/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                       <  SRR4302533.1001159/92‑1 (MQ=18)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                                      >  SRR4302533.292135/1‑94 (MQ=19)
    AAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCT                                                                    <  SRR4302533.2953665/94‑1 (MQ=21)
    AAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCT                                                                    <  SRR4302533.6229189/94‑1 (MQ=21)
     aggccatgcgcgggaagcggttgcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                   <  SRR4302533.433927/60‑1 (MQ=34)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                   >  SRR4302533.2757655/1‑94 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                   <  SRR4302533.1204246/94‑1 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                   >  SRR4302533.49854/1‑94 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                   <  SRR4302533.3758008/94‑1 (MQ=23)
      GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                  <  SRR4302533.5418036/94‑1 (MQ=25)
      GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                  <  SRR4302533.4117665/94‑1 (MQ=25)
      GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                  <  SRR4302533.243338/94‑1 (MQ=25)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                 <  SRR4302533.20499/94‑1 (MQ=26)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                 <  SRR4302533.3654174/94‑1 (MQ=26)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                 <  SRR4302533.5623384/94‑1 (MQ=26)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                 >  SRR4302533.39867/1‑94 (MQ=26)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                 <  SRR4302533.1174613/94‑1 (MQ=26)
         catgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGA                                                               >  SRR4302533.538534/31‑94 (MQ=42)
         catgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGA                                                               >  SRR4302533.3672547/31‑94 (MQ=42)
          atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                              <  SRR4302533.159236/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                              >  SRR4302533.835372/30‑94 (MQ=45)
           tgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                             <  SRR4302533.227141/66‑1 (MQ=47)
            gcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                            <  SRR4302533.5769847/67‑1 (MQ=49)
             cgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                                           <  SRR4302533.3219274/68‑1 (MQ=51)
              gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                                         <  SRR4302533.1249754/69‑1 (MQ=53)
              gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                                         >  SRR4302533.2823135/26‑94 (MQ=53)
               ctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG                                                        >  SRR4302533.3414928/25‑94 (MQ=55)
                tggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG                                                       <  SRR4302533.725472/71‑1 (MQ=57)
                tggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG                                                       >  SRR4302533.4347199/24‑94 (MQ=57)
                 ggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑G                                                    <  SRR4302533.1492162/72‑1 (MQ=56)
                  gaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GA                                                   <  SRR4302533.4639773/73‑1 (MQ=56)
                  gaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GA                                                   <  SRR4302533.1488020/73‑1 (MQ=56)
                  gaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GA                                                   >  SRR4302533.570631/22‑94 (MQ=56)
                   aagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GAA                                                  >  SRR4302533.3519055/21‑94 (MQ=55)
                     gcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAG                                                 <  SRR4302533.2615802/76‑1 (MQ=53)
                      cggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaagg                                                 <  SRR4302533.4427539/77‑7 (MQ=57)
                       ggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggc                                                <  SRR4302533.6042832/78‑8 (MQ=57)
                       ggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggc                                                >  SRR4302533.1248457/17‑87 (MQ=57)
                        gtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggct                                               <  SRR4302533.1951459/79‑9 (MQ=57)
                        gtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggct                                               >  SRR4302533.5714838/16‑86 (MQ=57)
                         tggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggctg                                              >  SRR4302533.43879/15‑85 (MQ=57)
                         tggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggctg                                              <  SRR4302533.355138/80‑10 (MQ=57)
                            cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCAT                                          >  SRR4302533.4625322/12‑94 (MQ=49)
                            cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCAT                                          >  SRR4302533.2915384/12‑94 (MQ=49)
                             gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATT                                         >  SRR4302533.3589461/11‑94 (MQ=49)
                               agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTAT                                       >  SRR4302533.3330090/9‑94 (MQ=48)
                               agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTAT                                       <  SRR4302533.2666254/86‑1 (MQ=48)
                                gaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATG                                      <  SRR4302533.1052833/87‑1 (MQ=49)
                                 aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGG                                     <  SRR4302533.2348022/88‑1 (MQ=51)
                                 aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGG                                     <  SRR4302533.4657144/88‑1 (MQ=51)
                                  AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGTCTGCATTATGGG                                    >  SRR4302533.3137206/1‑94 (MQ=48)
                                  AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGG                                    >  SRR4302533.2705082/1‑94 (MQ=50)
                                  AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGG                                    <  SRR4302533.1644244/94‑1 (MQ=50)
                                   ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGC                                   <  SRR4302533.2672427/94‑1 (MQ=53)
                                   ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGC                                   <  SRR4302533.2005247/94‑1 (MQ=53)
                                   ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGC                                   >  SRR4302533.4646378/1‑94 (MQ=53)
                                    ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCA                                  <  SRR4302533.4060026/91‑1 (MQ=57)
                                    ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCA                                  <  SRR4302533.849893/91‑1 (MQ=57)
                                    ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCA                                  >  SRR4302533.1760930/4‑94 (MQ=57)
                                     G‑GGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGT                                >  SRR4302533.1535130/1‑94 (MQ=60)
                                        GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                              >  SRR4302533.724731/1‑94 (MQ=60)
                                        GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                              >  SRR4302533.6199597/1‑94 (MQ=60)
                                         CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                             <  SRR4302533.5572877/94‑3 (MQ=60)
                                         CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                             >  SRR4302533.5783651/1‑92 (MQ=60)
                                           ACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgca                           <  SRR4302533.6277876/94‑5 (MQ=60)
                                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACC                         >  SRR4302533.1562424/4‑94 (MQ=60)
                                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACC                         <  SRR4302533.5237275/91‑1 (MQ=60)
                                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACC                         >  SRR4302533.5693748/4‑94 (MQ=60)
                                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACC                         >  SRR4302533.1025366/4‑94 (MQ=60)
                                              gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCA                        >  SRR4302533.97895/3‑94 (MQ=60)
                                              gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCA                        <  SRR4302533.3114813/92‑1 (MQ=60)
                                              C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAG                       >  SRR4302533.2061001/1‑94 (MQ=60)
                                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAATG‑CTGCATTATGGGCAGTGCGCACCAGC                      >  SRR4302533.2649175/1‑94 (MQ=60)
                                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC                      <  SRR4302533.5240768/94‑1 (MQ=60)
                                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC                      >  SRR4302533.5281497/1‑94 (MQ=60)
                                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC                      >  SRR4302533.6140799/1‑94 (MQ=60)
                                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC                      >  SRR4302533.1691103/1‑94 (MQ=60)
                                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC                      <  SRR4302533.5893172/94‑1 (MQ=60)
                                                 TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCG                     >  SRR4302533.311955/1‑94 (MQ=57)
                                                 TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCG                     <  SRR4302533.1754882/94‑1 (MQ=57)
                                                  GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGT                    <  SRR4302533.4739807/94‑1 (MQ=57)
                                                   AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTC                   >  SRR4302533.4802662/1‑94 (MQ=57)
                                                   AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTC                   <  SRR4302533.4651037/94‑1 (MQ=57)
                                                   AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTC                   >  SRR4302533.1798484/1‑94 (MQ=57)
                                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                 <  SRR4302533.5939014/94‑1 (MQ=60)
                                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                 <  SRR4302533.3707720/94‑1 (MQ=60)
                                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                 >  SRR4302533.5093451/1‑94 (MQ=60)
                                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                 >  SRR4302533.5056168/1‑94 (MQ=60)
                                                      GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGA                <  SRR4302533.5872538/94‑1 (MQ=60)
                                                          T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC           >  SRR4302533.331590/1‑94 (MQ=60)
                                                          T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC           >  SRR4302533.3030052/1‑94 (MQ=60)
                                                            ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC          <  SRR4302533.4518248/94‑1 (MQ=60)
                                                            ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC          >  SRR4302533.3945787/1‑94 (MQ=60)
                                                               GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC       <  SRR4302533.3420724/94‑1 (MQ=60)
                                                                CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA      >  SRR4302533.4302974/1‑94 (MQ=60)
                                                                  TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC  <  SRR4302533.2748331/94‑1 (MQ=60)
                                                                  TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC  >  SRR4302533.1452865/1‑94 (MQ=60)
                                                                  TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC  <  SRR4302533.1015955/94‑1 (MQ=60)
                                                                   |                                                                                                
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCC  >  NC_000913/4008370‑4008519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑