Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,469 +G intergenic (+36/‑197) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4661.G100.0% 531.7 / NA 164intergenic (+33/‑200)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (88/76);  total (88/76)

Only 100 of 172 total aligned reads displayed.

AACTGGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑A‑AA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGAC  >  NC_000913/4008399‑4008551
                                                                        |                                                                                           
actgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATggg                                                                     >  1:2688598/5‑93 (MQ=255)
actgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATggg                                                                     <  1:2656113/89‑1 (MQ=255)
actgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATggg                                                                     >  2:5517684/5‑93 (MQ=255)
actgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATggg                                                                     <  1:1993023/89‑1 (MQ=255)
actgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AA‑GTCTGCATTATggg                                                                     >  1:3118034/5‑93 (MQ=255)
 ctgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGc                                                                    <  2:1450881/90‑1 (MQ=255)
 ctgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGc                                                                    >  1:4618390/4‑93 (MQ=255)
 ctgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGc                                                                    <  1:4035461/90‑1 (MQ=255)
  tgggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCa                                                                   >  1:1750273/3‑93 (MQ=255)
   gggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAg                                                                  >  2:2095688/2‑93 (MQ=255)
    ggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGt                                                                 <  2:236929/93‑1 (MQ=255)
    ggCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGt                                                                 <  2:3176408/93‑1 (MQ=255)
      cTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTgc                                                               <  1:5539427/93‑2 (MQ=255)
      cTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTgc                                                               <  2:6200703/93‑2 (MQ=255)
      cTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTgc                                                               >  1:6162543/1‑92 (MQ=255)
      cTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTgc                                                               >  1:720482/1‑92 (MQ=255)
       tACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTgcg                                                              >  2:2417089/1‑91 (MQ=255)
       tACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTgcg                                                              >  1:5748970/1‑91 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCgcacc                                                          >  1:1019375/3‑93 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCgcacc                                                          >  2:3680671/3‑93 (MQ=255)
           gccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCgcacc                                                          >  2:398669/3‑93 (MQ=255)
             cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAg                                                        <  1:5209391/93‑1 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AATG‑CTGCATTATGGGCAGTGCGCACCAGc                                                       >  1:2632995/1‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGc                                                       >  2:351796/1‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGc                                                       <  2:3465136/93‑1 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGc                                                       >  2:2309666/1‑93 (MQ=255)
              tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGc                                                       >  1:1680921/1‑93 (MQ=255)
               gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGG‑A‑AG‑G‑CTGCATTATGGGCAGGGCGCACCACCg                                                      >  2:5814954/1‑93 (MQ=255)
               gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCg                                                      <  2:5172308/93‑1 (MQ=255)
               gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCg                                                      >  2:2994525/1‑93 (MQ=255)
               gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCg                                                      <  1:4711253/93‑1 (MQ=255)
                  ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt                                                   <  1:5903364/93‑1 (MQ=255)
                               tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                      >  2:4091185/5‑93 (MQ=255)
                                ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                     <  1:650055/90‑1 (MQ=255)
                                ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                     <  2:4996354/90‑1 (MQ=255)
                                 tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                    <  1:1010049/91‑1 (MQ=255)
                                  ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc                                   >  1:1444153/2‑93 (MQ=255)
                                     gACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                <  1:5351079/93‑1 (MQ=255)
                                       cGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGcg                              >  1:6236638/1‑92 (MQ=255)
                                        gggATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                            >  2:2186921/1‑93 (MQ=255)
                                         ggATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                           >  2:4893182/1‑92 (MQ=255)
                                           aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                         >  2:5920600/1‑90 (MQ=255)
                                           aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                         >  2:4369666/1‑90 (MQ=255)
                                           aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                         <  2:711517/93‑4 (MQ=255)
                                           aTGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                         <  2:5543605/93‑4 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                        >  1:2014182/1‑89 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                        >  1:3579576/1‑89 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                        >  1:6081650/1‑89 (MQ=255)
                                            tGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                        >  1:419879/1‑89 (MQ=255)
                                              aaCGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                       >  2:4270714/1‑93 (MQ=255)
                                              aaCGACGCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                       <  1:2064397/93‑1 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                      >  2:1913156/1‑93 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                      <  2:765178/93‑1 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                      <  1:3184550/93‑1 (MQ=255)
                                               acgacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                      >  1:4576222/1‑93 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                    <  1:2743801/93‑1 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                    >  2:5321920/1‑93 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                    <  1:4825751/93‑1 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                    <  1:3473692/93‑1 (MQ=255)
                                                 gacgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                    >  1:3576486/1‑93 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGGTTg                   >  1:5588423/1‑93 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                   >  1:709619/1‑93 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                   >  2:435163/1‑93 (MQ=255)
                                                  acgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                   <  2:5714979/93‑1 (MQ=255)
                                                   cgCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                  <  1:3051728/93‑1 (MQ=255)
                                                    gCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                 <  1:2493861/93‑1 (MQ=255)
                                                    gCCGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                 <  1:3313571/93‑1 (MQ=255)
                                                     ccGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                >  2:5347387/1‑93 (MQ=255)
                                                      cGAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc               <  2:612060/93‑1 (MQ=255)
                                                       gAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt              >  2:1499851/1‑93 (MQ=255)
                                                       gAAATGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt              >  2:5116289/1‑93 (MQ=255)
                                                        aaaTGCTTTCAATGGC‑‑GGGTA‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTACATCCCGAGCTGGAAGTGATTGGTACTa             <  2:1426094/93‑1 (MQ=255)
                                                        aaaTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa             >  1:3810435/1‑93 (MQ=255)
                                                        aaaTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa             >  1:5002868/1‑93 (MQ=255)
                                                        aaaTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa             <  1:2326490/93‑1 (MQ=255)
                                                        aaaTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa             >  2:4902070/1‑93 (MQ=255)
                                                         aaTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa            <  1:1989626/93‑1 (MQ=255)
                                                         aaTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa            <  2:2978707/93‑1 (MQ=255)
                                                          aTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt           <  1:1385819/93‑1 (MQ=255)
                                                          aTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt           <  1:5931535/93‑1 (MQ=255)
                                                          aTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt           >  1:973721/1‑93 (MQ=255)
                                                          aTGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt           >  1:1236449/1‑93 (MQ=255)
                                                           tGCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg          >  1:4356384/1‑93 (MQ=255)
                                                            gCTGTCGATGGCG‑GGG‑A‑AATG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc         <  1:1074795/93‑1 (MQ=255)
                                                            gCTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc         <  2:3015091/93‑1 (MQ=255)
                                                             cTGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc        >  2:6037881/1‑93 (MQ=255)
                                                              tGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg       >  1:1575772/1‑93 (MQ=255)
                                                              tGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg       >  2:5867509/1‑93 (MQ=255)
                                                              tGTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg       <  1:866946/93‑1 (MQ=255)
                                                               gTCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga      <  1:3292984/93‑1 (MQ=255)
                                                                tCGGTGGCG‑GGG‑A‑AAGG‑CTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGGAAGTGATTGGTACTAATGCcgac     <  2:4782770/93‑1 (MQ=255)
                                                                tCGATGGCG‑GGG‑ATAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac     <  1:2163614/93‑1 (MQ=255)
                                                                tCGATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac     <  1:5511444/93‑1 (MQ=255)
                                                                  gATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga   >  2:447040/1‑93 (MQ=255)
                                                                  gATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga   <  2:38650/93‑1 (MQ=255)
                                                                  gATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga   <  2:3320626/93‑1 (MQ=255)
                                                                  gATGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga   <  1:1526869/93‑1 (MQ=255)
                                                                   aTGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac  >  1:3021932/1‑93 (MQ=255)
                                                                   aTGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac  <  1:3621465/93‑1 (MQ=255)
                                                                   aTGGCG‑GGG‑A‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac  >  1:6233158/1‑93 (MQ=255)
                                                                        |                                                                                           
AACTGGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑A‑AA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGAC  >  NC_000913/4008399‑4008551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGCTGGAAGGCTGGCGAACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG  >  NC_000913/4008381‑4008560
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tggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG                                                                                                  >  SRR4302533.4347199/24‑94 (MQ=57)
tggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG                                                                                                  <  SRR4302533.725472/71‑1 (MQ=57)
 ggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑G                                                                                               <  SRR4302533.1492162/72‑1 (MQ=56)
  gaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GA                                                                                              >  SRR4302533.570631/22‑94 (MQ=56)
  gaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GA                                                                                              <  SRR4302533.4639773/73‑1 (MQ=56)
  gaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GA                                                                                              <  SRR4302533.1488020/73‑1 (MQ=56)
   aagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑GAA                                                                                             >  SRR4302533.3519055/21‑94 (MQ=55)
     gcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAA‑G                                                                                           <  SRR4302533.2615802/76‑1 (MQ=53)
      cggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaagg                                                                                            <  SRR4302533.4427539/77‑7 (MQ=57)
       ggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggc                                                                                           <  SRR4302533.6042832/78‑8 (MQ=57)
       ggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggc                                                                                           >  SRR4302533.1248457/17‑87 (MQ=57)
        gtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggct                                                                                          >  SRR4302533.5714838/16‑86 (MQ=57)
        gtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggct                                                                                          <  SRR4302533.1951459/79‑9 (MQ=57)
         tggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggctg                                                                                         >  SRR4302533.43879/15‑85 (MQ=57)
         tggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGgaaaggctg                                                                                         <  SRR4302533.355138/80‑10 (MQ=57)
            cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCAT                                                                                    >  SRR4302533.4625322/12‑94 (MQ=49)
            cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCAT                                                                                    >  SRR4302533.2915384/12‑94 (MQ=49)
             gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATT                                                                                   >  SRR4302533.3589461/11‑94 (MQ=49)
               agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTAT                                                                                 >  SRR4302533.3330090/9‑94 (MQ=48)
               agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTAT                                                                                 <  SRR4302533.2666254/86‑1 (MQ=48)
                gaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATG                                                                                <  SRR4302533.1052833/87‑1 (MQ=49)
                 aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGG                                                                               <  SRR4302533.2348022/88‑1 (MQ=51)
                 aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGG                                                                               <  SRR4302533.4657144/88‑1 (MQ=51)
                  AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAA‑GTCTGCATTATGGG                                                                              >  SRR4302533.3137206/1‑94 (MQ=48)
                  AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGG                                                                              >  SRR4302533.2705082/1‑94 (MQ=50)
                  AACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGG                                                                              <  SRR4302533.1644244/94‑1 (MQ=50)
                   ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGC                                                                             <  SRR4302533.2672427/94‑1 (MQ=53)
                   ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGC                                                                             <  SRR4302533.2005247/94‑1 (MQ=53)
                   ACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGC                                                                             >  SRR4302533.4646378/1‑94 (MQ=53)
                    ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCA                                                                            >  SRR4302533.1760930/4‑94 (MQ=57)
                    ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCA                                                                            <  SRR4302533.4060026/91‑1 (MQ=57)
                    ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCA                                                                            <  SRR4302533.849893/91‑1 (MQ=57)
                     G‑GGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGT                                                                          >  SRR4302533.1535130/1‑94 (MQ=60)
                        GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGC                                                                        >  SRR4302533.724731/1‑94 (MQ=60)
                        GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGC                                                                        >  SRR4302533.6199597/1‑94 (MQ=60)
                         CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGcg                                                                       <  SRR4302533.5572877/94‑3 (MQ=60)
                         CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGcg                                                                       >  SRR4302533.5783651/1‑92 (MQ=60)
                           ACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGcgca                                                                     <  SRR4302533.6277876/94‑5 (MQ=60)
                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                   <  SRR4302533.5237275/91‑1 (MQ=60)
                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                   >  SRR4302533.5693748/4‑94 (MQ=60)
                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                   >  SRR4302533.1025366/4‑94 (MQ=60)
                             agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACC                                                                   >  SRR4302533.1562424/4‑94 (MQ=60)
                              gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCA                                                                  >  SRR4302533.97895/3‑94 (MQ=60)
                              gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCA                                                                  <  SRR4302533.3114813/92‑1 (MQ=60)
                              C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAG                                                                 >  SRR4302533.2061001/1‑94 (MQ=60)
                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAT‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                >  SRR4302533.2649175/1‑94 (MQ=60)
                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                <  SRR4302533.5240768/94‑1 (MQ=60)
                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                >  SRR4302533.5281497/1‑94 (MQ=60)
                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                >  SRR4302533.1691103/1‑94 (MQ=60)
                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                <  SRR4302533.5893172/94‑1 (MQ=60)
                                CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC                                                                >  SRR4302533.6140799/1‑94 (MQ=60)
                                 TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCG                                                               <  SRR4302533.1754882/94‑1 (MQ=57)
                                 TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCG                                                               >  SRR4302533.311955/1‑94 (MQ=57)
                                  GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGT                                                              <  SRR4302533.4739807/94‑1 (MQ=57)
                                   AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                             >  SRR4302533.4802662/1‑94 (MQ=57)
                                   AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                             >  SRR4302533.1798484/1‑94 (MQ=57)
                                   AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC                                                             <  SRR4302533.4651037/94‑1 (MQ=57)
                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                           >  SRR4302533.5093451/1‑94 (MQ=60)
                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                           >  SRR4302533.5056168/1‑94 (MQ=60)
                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                           <  SRR4302533.5939014/94‑1 (MQ=60)
                                     GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                                                           <  SRR4302533.3707720/94‑1 (MQ=60)
                                      GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGA                                                          <  SRR4302533.5872538/94‑1 (MQ=60)
                                         tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                       >  SRR4302533.1405984/14‑94 (MQ=60)
                                         tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                       <  SRR4302533.6149351/81‑1 (MQ=60)
                                         tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAG                                                       >  SRR4302533.3367185/14‑94 (MQ=60)
                                          gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                      <  SRR4302533.4321293/82‑1 (MQ=60)
                                          gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGA                                                      <  SRR4302533.251519/82‑1 (MQ=60)
                                          T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                     >  SRR4302533.331590/1‑94 (MQ=60)
                                          T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC                                                     >  SRR4302533.3030052/1‑94 (MQ=60)
                                            ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                    >  SRR4302533.3945787/1‑94 (MQ=60)
                                            ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC                                                    <  SRR4302533.4518248/94‑1 (MQ=60)
                                               GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTC                                                 <  SRR4302533.3420724/94‑1 (MQ=60)
                                                CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA                                                >  SRR4302533.4302974/1‑94 (MQ=60)
                                                 gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAT                                               <  SRR4302533.4771578/89‑1 (MQ=60)
                                                  TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                            <  SRR4302533.2748331/94‑1 (MQ=60)
                                                  TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                            <  SRR4302533.1015955/94‑1 (MQ=60)
                                                  TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC                                            >  SRR4302533.1452865/1‑94 (MQ=60)
                                                   ttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCC                                             <  SRR4302533.653891/91‑1 (MQ=60)
                                                      GTGACGGGATGAACGGCGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGA                                          <  SRR4302533.638927/94‑1 (MQ=60)
                                                        GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                        <  SRR4302533.5383189/94‑1 (MQ=60)
                                                        GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                        <  SRR4302533.4875153/94‑1 (MQ=60)
                                                         ACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCG                                       >  SRR4302533.6273983/1‑94 (MQ=60)
                                                          CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                     <  SRR4302533.654173/94‑1 (MQ=60)
                                                            GGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                   >  SRR4302533.2700209/1‑94 (MQ=60)
                                                             GATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                  <  SRR4302533.1163654/94‑4 (MQ=60)
                                                              ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                  >  SRR4302533.422394/1‑94 (MQ=60)
                                                              ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                  >  SRR4302533.3601632/1‑94 (MQ=60)
                                                              ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                  >  SRR4302533.2026590/1‑94 (MQ=60)
                                                              ATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                  >  SRR4302533.6118427/1‑94 (MQ=60)
                                                               TGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                 <  SRR4302533.2016301/94‑1 (MQ=60)
                                                               TGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                 <  SRR4302533.2112503/94‑1 (MQ=60)
                                                               TGAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Gggagt                                >  SRR4302533.2259585/1‑89 (MQ=60)
                                                                GAACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                <  SRR4302533.404968/94‑1 (MQ=60)
                                                                 AACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                               <  SRR4302533.2077121/94‑1 (MQ=60)
                                                                 AACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                               >  SRR4302533.4603957/1‑94 (MQ=60)
                                                                  ACGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                              <  SRR4302533.3204137/94‑1 (MQ=60)
                                                                   CGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                             >  SRR4302533.3598527/1‑94 (MQ=60)
                                                                   CGACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                             >  SRR4302533.296066/1‑94 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGGTTG                            >  SRR4302533.5622187/1‑94 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                            >  SRR4302533.713810/1‑94 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                            <  SRR4302533.3495127/94‑1 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                            <  SRR4302533.237587/94‑1 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                            >  SRR4302533.315784/1‑94 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                            <  SRR4302533.2760624/94‑1 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                            <  SRR4302533.4854994/94‑1 (MQ=60)
                                                                    GACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                            <  SRR4302533.6030519/94‑1 (MQ=60)
                                                                     ACGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                           <  SRR4302533.560469/94‑1 (MQ=60)
                                                                      CGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                          <  SRR4302533.3070417/94‑1 (MQ=60)
                                                                      CGCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                          <  SRR4302533.3903849/94‑1 (MQ=60)
                                                                       GCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                         <  SRR4302533.2509245/94‑1 (MQ=60)
                                                                       GCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                         >  SRR4302533.3587940/1‑94 (MQ=60)
                                                                       GCCGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                         <  SRR4302533.3334030/94‑1 (MQ=60)
                                                                         CGAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTACGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                       >  SRR4302533.3409008/1‑94 (MQ=60)
                                                                          GAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                      >  SRR4302533.2578166/1‑94 (MQ=60)
                                                                          GAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                      >  SRR4302533.5033191/1‑94 (MQ=60)
                                                                          GAAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                      >  SRR4302533.3833740/1‑94 (MQ=60)
                                                                           AAATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                     <  SRR4302533.2340893/94‑1 (MQ=60)
                                                                            AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                    >  SRR4302533.979437/1‑94 (MQ=60)
                                                                            AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                    >  SRR4302533.1066069/1‑94 (MQ=60)
                                                                            AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                    <  SRR4302533.4515122/94‑1 (MQ=60)
                                                                            AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                    >  SRR4302533.1243837/1‑94 (MQ=60)
                                                                            AATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                    <  SRR4302533.2001805/94‑1 (MQ=60)
                                                                             ATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                   >  SRR4302533.4382678/1‑94 (MQ=60)
                                                                             ATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                   <  SRR4302533.1394158/94‑1 (MQ=60)
                                                                             ATGCTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                   <  SRR4302533.5967395/94‑1 (MQ=60)
                                                                               GCTGTCGATGGC‑GGGGAAAT‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                 <  SRR4302533.1081131/94‑1 (MQ=60)
                                                                                CTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                >  SRR4302533.1585259/1‑94 (MQ=60)
                                                                                CTGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                >  SRR4302533.3630606/1‑94 (MQ=60)
                                                                                 TGTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA               <  SRR4302533.872027/94‑1 (MQ=60)
                                                                                  GTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC              <  SRR4302533.5462091/94‑1 (MQ=60)
                                                                                  GTCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC              <  SRR4302533.3313310/94‑1 (MQ=60)
                                                                                   TCGATGGC‑GGGGATAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG             <  SRR4302533.2176926/94‑1 (MQ=60)
                                                                                   TCGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG             <  SRR4302533.5544718/94‑1 (MQ=60)
                                                                                    CGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA            >  SRR4302533.4281337/1‑94 (MQ=60)
                                                                                    CGATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA            >  SRR4302533.3244615/1‑94 (MQ=60)
                                                                                     GATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC           <  SRR4302533.1536061/94‑1 (MQ=60)
                                                                                     GATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC           >  SRR4302533.6270481/1‑94 (MQ=60)
                                                                                     GATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC           >  SRR4302533.3040421/1‑94 (MQ=60)
                                                                                     GATGGC‑GGGGAAAG‑G‑CTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC           <  SRR4302533.1434815/94‑1 (MQ=60)
                                                                                      ATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG          >  SRR4302533.4061225/1‑94 (MQ=60)
                                                                                      ATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG          <  SRR4302533.6248425/94‑1 (MQ=60)
                                                                                      ATGGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG          <  SRR4302533.3643750/94‑1 (MQ=60)
                                                                                        GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG        <  SRR4302533.3079970/94‑1 (MQ=60)
                                                                                        GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG        <  SRR4302533.5846030/94‑1 (MQ=60)
                                                                                        GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG        <  SRR4302533.5211778/94‑1 (MQ=60)
                                                                                        GGC‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG        <  SRR4302533.3415015/94‑1 (MQ=60)
                                                                                            GGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGC    >  SRR4302533.3640954/1‑94 (MQ=60)
                                                                                             GG‑‑AAAGG‑CTGCATTATGGGCAGTTCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACCACGCGGTGCC   >  SRR4302533.1525870/1‑94 (MQ=60)
                                                                                             GG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC   >  SRR4302533.512215/1‑94 (MQ=60)
                                                                                              G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG  >  SRR4302533.4097476/1‑94 (MQ=60)
                                                                                              |                                                                                                
TGCGCTGGAAGGCTGGCGAACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG  >  NC_000913/4008381‑4008560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑