Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,717	+G	coding (3/999 nt)	ysgA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,717	1	.	G	100.0%	772.9 / NA	241	M1M (ATG→ATC) †	ysgA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (136/105); total (136/105)

Only 100 of 252 total aligned reads displayed.

TAAGCAGGCATGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

 aaGCAGGCATGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcg                                                                                         >  1:1053188/1‑92 (MQ=255)

   gCAGGCATGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcgat                                                                                       >  2:4698324/1‑90 (MQ=255)

      ggCATGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                    >  2:1716103/1‑93 (MQ=255)

       gCATGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcag                                                                                   >  2:4307555/1‑93 (MQ=255)

       gCATGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcag                                                                                   >  1:4563723/1‑93 (MQ=255)

         aggTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcagca                                                                                 >  2:6208759/3‑93 (MQ=255)

         aTGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcagca                                                                                 >  2:3363761/1‑93 (MQ=255)

          tGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                                >  1:6005490/1‑93 (MQ=255)

          tGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                                >  2:3987379/1‑93 (MQ=255)

          tGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                                >  2:5691269/1‑93 (MQ=255)

           tttttCCCCTTAGTGAAGGGATAGGGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                                <  1:5105372/89‑1 (MQ=255)

           gTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGATGCGATGCAGCAgg                                                                               >  1:4605660/1‑93 (MQ=255)

           gTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAgg                                                                               >  2:5175171/1‑93 (MQ=255)

            ttATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAggg                                                                              >  2:1764697/1‑92 (MQ=255)

            ttATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGt                                                                              >  2:2257860/1‑93 (MQ=255)

            ttATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCACGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAggg                                                                              >  1:3388707/1‑92 (MQ=255)

              aTCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGc                                                                            >  1:3922734/1‑93 (MQ=255)

               tCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCa                                                                           >  1:4738005/1‑93 (MQ=255)

               cccccTT‑GGGAAGGGATAGATGTGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCa                                                                           <  2:3109795/92‑1 (MQ=255)

                 cccTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCaaa                                                                         >  1:2892877/1‑93 (MQ=255)

                 cccTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGCGCaaa                                                                         >  1:467495/1‑93 (MQ=255)

                   cTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                       >  2:4480194/1‑93 (MQ=255)

                    tt‑GTGAAGGGGTAGAGGGGAAGCCCGCCACAATCCCGTCGTCCCGGGGCTGAACGATGGGCGGAGCGAGAGGCGAGGCAGCAGGGGGaaaatc                                                                      >  2:1385034/1‑90 (MQ=255)

                    tt‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATcc                                                                      >  1:1546752/1‑93 (MQ=255)

                    tt‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATcc                                                                      >  2:1244758/1‑93 (MQ=255)

                     t‑gtgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCa                                                                     >  2:2721034/1‑93 (MQ=255)

                     t‑gtgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCa                                                                     <  1:1012908/93‑1 (MQ=255)

                       gtgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAg                                                                    >  2:3659370/1‑93 (MQ=255)

                       gtgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAg                                                                    >  2:1507529/1‑93 (MQ=255)

                        tgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGa                                                                   >  1:1268887/1‑93 (MQ=255)

                         gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                  >  2:1194267/1‑93 (MQ=255)

                         gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                  >  1:2663148/1‑93 (MQ=255)

                         gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                  >  1:6211926/1‑93 (MQ=255)

                         gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                  >  1:5351288/1‑93 (MQ=255)

                          gaGGGAGAAAGATGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAtt                                                                 <  2:2959219/92‑1 (MQ=255)

                          aaGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAtt                                                                 >  2:4796829/1‑93 (MQ=255)

                          aaGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAtt                                                                 >  1:552874/1‑93 (MQ=255)

                          aaGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAtt                                                                 >  1:520819/1‑93 (MQ=255)

                           aGGGATAGAGTGAAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttg                                                                <  2:2568088/93‑1 (MQ=255)

                             gaaTAGGGGTGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgtt                                                              <  2:2610590/91‑1 (MQ=255)

                               aTAGGGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgt                                                            <  2:2900737/93‑1 (MQ=255)

                               aTAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgt                                                            <  1:2009958/93‑1 (MQ=255)

                               aTAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgt                                                            >  1:2271270/1‑93 (MQ=255)

                               aTAGAGGGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgt                                                            <  1:5916554/93‑1 (MQ=255)

                                  gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgtt                                                         >  2:2084642/1‑93 (MQ=255)

                                   gggTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgttg                                                        <  1:3192432/92‑1 (MQ=255)

                                      ggAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGttgttg                                                     <  1:5491758/92‑1 (MQ=255)

                                        aaGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGcc                                                   >  2:2340518/1‑93 (MQ=255)

                                         agaccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCa                                                  <  2:4016829/90‑1 (MQ=255)

                                         accccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCa                                                  <  1:2807630/91‑1 (MQ=255)

                                         aGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCa                                                  <  2:4388302/93‑1 (MQ=255)

                                            ccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAACCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATgg                                               >  2:6268990/1‑93 (MQ=255)

                                             cGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGt                                              <  1:2363073/93‑1 (MQ=255)

                                             cGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGt                                              <  1:3741350/93‑1 (MQ=255)

                                              gCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGGGCAAATCCAGCTTGTTGTGTTGTTGCCATGGTa                                             >  2:2835006/1‑93 (MQ=255)

                                               ccACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAt                                            <  1:5639904/93‑1 (MQ=255)

                                                cacaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAtt                                           >  1:4032322/1‑93 (MQ=255)

                                                cacaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAtt                                           <  2:5631175/93‑1 (MQ=255)

                                                  caATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtct                                         <  1:4358166/93‑1 (MQ=255)

                                                   aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                        >  1:2778438/1‑93 (MQ=255)

                                                    aTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTcc                                       <  2:247181/93‑1 (MQ=255)

                                                      cgcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGt                                     >  1:5227648/1‑93 (MQ=255)

                                                      cgcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGt                                     <  2:3775272/93‑1 (MQ=255)

                                                       gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑a                                   >  2:5336849/1‑93 (MQ=255)

                                                       gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATACTCCGT‑a                                   >  2:440645/1‑93 (MQ=255)

                                                         gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑cc                                >  2:5755551/1‑93 (MQ=255)

                                                           cgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑ccct                              <  1:851256/93‑3 (MQ=255)

                                                             tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑ccctta                            <  2:5499826/93‑5 (MQ=255)

                                                               cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTtata                           >  1:684476/1‑93 (MQ=255)

                                                               cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTtata                           >  2:4959555/1‑93 (MQ=255)

                                                               cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTtata                           >  2:654495/1‑93 (MQ=255)

                                                                ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATaa                          >  2:280224/1‑93 (MQ=255)

                                                                  ggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATaaaa                        <  1:4170853/93‑1 (MQ=255)

                                                                   gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATaaaaa                       <  1:5328840/93‑1 (MQ=255)

                                                                   gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATaaaaa                       >  2:5829914/1‑93 (MQ=255)

                                                                     cTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATg                     >  1:4407772/1‑93 (MQ=255)

                                                                      tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGt                    >  1:964059/1‑93 (MQ=255)

                                                                      tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGt                    <  1:4324442/93‑1 (MQ=255)

                                                                        aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTg                  <  1:1234062/93‑1 (MQ=255)

                                                                        aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTg                  >  2:4877010/1‑93 (MQ=255)

                                                                         aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTgc                 <  2:3213555/93‑1 (MQ=255)

                                                                         aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTgc                 >  1:2414814/1‑93 (MQ=255)

                                                                         aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTgc                 <  1:5755426/93‑1 (MQ=255)

                                                                         aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTgc                 <  2:598285/93‑1 (MQ=255)

                                                                          cGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTgcg                >  2:3456413/1‑93 (MQ=255)

                                                                             tGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCaa             <  1:4514309/93‑1 (MQ=255)

                                                                             tGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCaa             >  1:3165076/1‑93 (MQ=255)

                                                                             tGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCaa             <  1:2516158/93‑1 (MQ=255)

                                                                                tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGt          <  2:1097037/93‑1 (MQ=255)

                                                                                tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGt          <  2:1568641/93‑1 (MQ=255)

                                                                                tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGt          <  1:6174032/93‑1 (MQ=255)

                                                                                 cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGtt         <  2:5169311/93‑1 (MQ=255)

                                                                                 cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGtt         <  1:5016819/93‑1 (MQ=255)

                                                                                 cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGtt         <  1:1771338/93‑1 (MQ=255)

                                                                                  gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTGC‑CCTTATAAAAATGTTGCGCAATGTTa        <  2:2028770/93‑1 (MQ=255)

                                                                                  gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTa        >  1:3548567/1‑93 (MQ=255)

                                                                                  gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTa        >  2:896223/1‑93 (MQ=255)

                                                                                       gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACtat   >  2:4536217/1‑93 (MQ=255)

                                                                                        agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACtata  <  1:3327054/93‑1 (MQ=255)

                                                                                        agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACtata  >  1:1678927/1‑93 (MQ=255)

TAAGCAGGCATGTTATCCCCTT‑GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAGCAGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013625‑4013809

TAAGCAGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑G                                                                                               >  SRR4302533.1059391/1‑94 (MQ=60)

 AAGCAGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCga                                                                                               >  SRR4302533.4431614/1‑92 (MQ=60)

    CAGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                            >  SRR4302533.4209922/1‑94 (MQ=60)

    CAGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                            >  SRR4302533.5408979/1‑94 (MQ=60)

     AGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                           >  SRR4302533.3049475/1‑94 (MQ=60)

     AGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                           >  SRR4302533.1375612/1‑94 (MQ=60)

      GGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                          >  SRR4302533.4591397/1‑94 (MQ=60)

        CTTGTTATCCCT‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                        <  SRR4302533.4353312/94‑1 (MQ=60)

         cagtttctcccgtggtgagGGGATAGAGGTGACCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                        <  SRR4302533.679121/75‑1 (MQ=60)

         ATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                       >  SRR4302533.6041878/1‑94 (MQ=60)

         ATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                       <  SRR4302533.3196187/94‑1 (MQ=60)

          TTTTACCCCC‑TTGTGAGGGAATAGAGGTGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                      <  SRR4302533.3246227/94‑1 (MQ=60)

          TTTTACCCCC‑TTGTGAAGGGATAGAGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                      <  SRR4302533.24932/94‑1 (MQ=60)

          TGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGATGCGATGCAGCAGG                                                                                      >  SRR4302533.4633560/1‑94 (MQ=60)

           tttttccccttaGTGAAGGGATAGGGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                      <  SRR4302533.5136211/82‑1 (MQ=60)

           ttttctcccttggtgaggggataggggtgagcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                      <  SRR4302533.1100413/62‑1 (MQ=60)

           GTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCACGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGG                                                                                     >  SRR4302533.3409632/1‑94 (MQ=60)

            tgtctccccgggtgaaggaaaaatggggcgcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                     <  SRR4302533.4106085/63‑1 (MQ=60)

            TTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTG                                                                                    >  SRR4302533.3792342/1‑94 (MQ=60)

             TATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGC                                                                                   >  SRR4302533.3946686/1‑94 (MQ=60)

              ATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCA                                                                                  >  SRR4302533.6308065/1‑94 (MQ=60)

              ATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCA                                                                                  >  SRR4302533.4766734/1‑94 (MQ=60)

               accgcctggtggAGGGATAGAGGAGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCA                                                                                  <  SRR4302533.3991775/82‑1 (MQ=60)

               TCCCCTTTGTGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCA                                                                                  <  SRR4302533.5557974/94‑1 (MQ=60)

                CCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAA                                                                                >  SRR4302533.2910657/1‑94 (MQ=60)

                CCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGCGCAAA                                                                                >  SRR4302533.470287/1‑94 (MQ=60)

                  CC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATC                                                                              >  SRR4302533.2777854/1‑94 (MQ=60)

                  CC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATC                                                                              >  SRR4302533.1181727/1‑94 (MQ=60)

                   C‑TTGTGAGGGGATAGAGGGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCC                                                                             <  SRR4302533.4704099/94‑1 (MQ=60)

                   C‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCC                                                                             >  SRR4302533.1556073/1‑94 (MQ=60)

                    ctggggagggaaaaggGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCC                                                                             <  SRR4302533.4321173/78‑1 (MQ=60)

                     tggtaagggtatggaggagacGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCA                                                                            <  SRR4302533.303535/73‑1 (MQ=60)

                     TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCA                                                                            >  SRR4302533.332164/1‑94 (MQ=60)

                      TGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                           >  SRR4302533.4345904/1‑94 (MQ=60)

                      TGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                           >  SRR4302533.523258/1‑94 (MQ=60)

                      TGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                           <  SRR4302533.1018838/94‑1 (MQ=60)

                       GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGA                                                                          >  SRR4302533.1276483/1‑94 (MQ=60)

                       GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGA                                                                          >  SRR4302533.3435196/1‑94 (MQ=60)

                        TGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT                                                                         >  SRR4302533.2679498/1‑94 (MQ=60)

                        TGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT                                                                         >  SRR4302533.5383398/1‑94 (MQ=60)

                        TGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT                                                                         >  SRR4302533.6249177/1‑94 (MQ=60)

                        TGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT                                                                         >  SRR4302533.6130168/1‑94 (MQ=60)

                        TGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT                                                                         >  SRR4302533.6029806/1‑94 (MQ=60)

                         GAGGGGAGAGGGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                        <  SRR4302533.462186/94‑1 (MQ=60)

                         GAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                        >  SRR4302533.523909/1‑94 (MQ=60)

                         GAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                        >  SRR4302533.556150/1‑94 (MQ=60)

                          aggggagagagggaacGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTG                                                                       <  SRR4302533.5329015/78‑1 (MQ=60)

                           AGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGT                                                                      >  SRR4302533.5623286/1‑94 (MQ=60)

                           AGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGT                                                                      >  SRR4302533.238345/1‑94 (MQ=60)

                             gaaagaAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTG                                                                    <  SRR4302533.4232125/88‑1 (MQ=60)

                              GATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                   >  SRR4302533.2285325/1‑94 (MQ=60)

                              GATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                   >  SRR4302533.2172768/1‑94 (MQ=60)

                               ATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                  <  SRR4302533.2022340/94‑1 (MQ=60)

                               ATAGAGGGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                  <  SRR4302533.5952311/94‑1 (MQ=60)

                               ATAGAGGGGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                  <  SRR4302533.409671/94‑1 (MQ=60)

                                 AGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGCGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                                >  SRR4302533.3001605/1‑94 (MQ=60)

                                 AGAGGGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                                <  SRR4302533.1761672/94‑1 (MQ=60)

                                  GAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTG                                                               <  SRR4302533.949991/94‑1 (MQ=60)

                                  GAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTG                                                               <  SRR4302533.6146461/94‑1 (MQ=60)

                                  GAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTG                                                               <  SRR4302533.3943197/94‑1 (MQ=60)

                                   aggagagcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                              <  SRR4302533.2646075/86‑1 (MQ=60)

                                   GGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                              <  SRR4302533.3212071/94‑1 (MQ=60)

                                   AGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                              >  SRR4302533.4403514/1‑94 (MQ=60)

                                   AGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                              >  SRR4302533.5011563/1‑94 (MQ=60)

                                      GGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGC                                                           <  SRR4302533.5524886/94‑1 (MQ=60)

                                       GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                          >  SRR4302533.2425202/1‑94 (MQ=60)

                                       GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                          >  SRR4302533.3572469/1‑94 (MQ=60)

                                         ACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                        <  SRR4302533.2824908/94‑1 (MQ=60)

                                             CGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTA                                                    <  SRR4302533.3764293/94‑1 (MQ=60)

                                             CGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTA                                                    <  SRR4302533.2377692/94‑1 (MQ=60)

                                              GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTTTTGTGTTGTTGCCATGGTAT                                                   >  SRR4302533.5743664/1‑94 (MQ=60)

                                              GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                   >  SRR4302533.2568806/1‑94 (MQ=60)

                                              GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                   >  SRR4302533.552232/1‑94 (MQ=60)

                                              GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                   <  SRR4302533.5895096/94‑1 (MQ=60)

                                              GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                   <  SRR4302533.586219/94‑1 (MQ=60)

                                               CCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATT                                                  >  SRR4302533.4056868/1‑94 (MQ=60)

                                               CCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATT                                                  <  SRR4302533.5673996/94‑1 (MQ=60)

                                                CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                 >  SRR4302533.2363052/1‑94 (MQ=60)

                                                CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                 >  SRR4302533.4447897/1‑94 (MQ=60)

                                                 ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                                <  SRR4302533.5239592/94‑1 (MQ=60)

                                                 ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                                <  SRR4302533.4553211/94‑1 (MQ=60)

                                                 ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                                >  SRR4302533.5308913/1‑94 (MQ=60)

                                                 ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                                >  SRR4302533.4342248/1‑94 (MQ=60)

                                                  CAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTC                                               <  SRR4302533.4384472/94‑1 (MQ=60)

                                                  CAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTC                                               >  SRR4302533.2795504/1‑94 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                              <  SRR4302533.1265018/94‑1 (MQ=60)

                                                     TCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT                                            >  SRR4302533.5259101/1‑94 (MQ=60)

                                                      CGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A                                          >  SRR4302533.4547620/1‑94 (MQ=60)

                                                           CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCctt                                    >  SRR4302533.5158267/1‑91 (MQ=60)

                                                           CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCctt                                    <  SRR4302533.856239/94‑4 (MQ=60)

                                                            GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTA                                    >  SRR4302533.920412/1‑94 (MQ=60)

                                                             TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                   <  SRR4302533.1502382/94‑1 (MQ=60)

                                                             TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                   <  SRR4302533.289915/94‑1 (MQ=60)

                                                             TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTAT                                   >  SRR4302533.2817580/1‑94 (MQ=60)

                                                              CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                  >  SRR4302533.688525/1‑94 (MQ=60)

                                                              CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                  <  SRR4302533.6166285/94‑1 (MQ=60)

                                                              CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGGGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATA                                  >  SRR4302533.2003581/1‑94 (MQ=60)

                                                                GGNGNCNGNACGATGGTCGAAGNGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAA                                <  SRR4302533.4509231/94‑1 (MQ=60)

                                                                GGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAA                                >  SRR4302533.3409081/1‑94 (MQ=60)

                                                                 GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAA                               >  SRR4302533.6277442/1‑94 (MQ=60)

                                                                  GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAA                              <  SRR4302533.4196124/94‑1 (MQ=60)

                                                                  GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGCTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAA                              >  SRR4302533.5373267/1‑94 (MQ=60)

                                                                   GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAAT                             <  SRR4302533.5360775/94‑1 (MQ=60)

                                                                    TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATG                            >  SRR4302533.2649020/1‑94 (MQ=60)

                                                                    TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATG                            >  SRR4302533.4434389/1‑94 (MQ=60)

                                                                     CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGT                           >  SRR4302533.969721/1‑94 (MQ=60)

                                                                     CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGT                           >  SRR4302533.1644966/1‑94 (MQ=60)

                                                                      TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                          <  SRR4302533.6221205/94‑1 (MQ=60)

                                                                      TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                          <  SRR4302533.4669326/94‑1 (MQ=60)

                                                                      TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                          <  SRR4302533.4350484/94‑1 (MQ=60)

                                                                      TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTT                          >  SRR4302533.5524535/1‑94 (MQ=60)

                                                                       GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTG                         >  SRR4302533.4907600/1‑94 (MQ=60)

                                                                        AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTCC‑CCTTATAAAAATGTTGC                        <  SRR4302533.2818188/94‑1 (MQ=60)

                                                                        AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                        <  SRR4302533.1241442/94‑1 (MQ=60)

                                                                        AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGC                        >  SRR4302533.2429730/1‑94 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                       >  SRR4302533.4128225/1‑94 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                       <  SRR4302533.5790154/94‑1 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                       <  SRR4302533.2822073/94‑1 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCG                       >  SRR4302533.5610288/1‑94 (MQ=60)

                                                                           GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCA                     >  SRR4302533.1034889/1‑94 (MQ=60)

                                                                            ATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAA                    >  SRR4302533.3184554/1‑94 (MQ=60)

                                                                             TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAAT                   <  SRR4302533.4541646/94‑1 (MQ=60)

                                                                             TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAAT                   <  SRR4302533.2531663/94‑1 (MQ=60)

                                                                                TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTT                <  SRR4302533.6211135/94‑1 (MQ=60)

                                                                                TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTT                <  SRR4302533.4691819/94‑1 (MQ=60)

                                                                                 CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA               >  SRR4302533.3570435/1‑94 (MQ=60)

                                                                                 CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA               <  SRR4302533.5047211/94‑1 (MQ=60)

                                                                                 CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA               <  SRR4302533.1759407/94‑1 (MQ=60)

                                                                                 CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTA               <  SRR4302533.1782120/94‑1 (MQ=60)

                                                                                   AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAAC             >  SRR4302533.2236292/1‑94 (MQ=60)

                                                                                    AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACT            >  SRR4302533.123520/1‑94 (MQ=60)

                                                                                       GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302533.5099284/1‑94 (MQ=60)

                                                                                       GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302533.1689096/1‑94 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG        <  SRR4302533.3462660/94‑1 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG        <  SRR4302533.806535/94‑1 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG        >  SRR4302533.4981529/1‑94 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG        >  SRR4302533.3762779/1‑94 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAG        <  SRR4302533.3347612/94‑1 (MQ=60)

                                                                                         GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT       >  SRR4302533.5142117/1‑94 (MQ=60)

                                                                                         GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT       >  SRR4302533.2228510/1‑94 (MQ=60)

                                                                                         GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT       <  SRR4302533.1613249/94‑1 (MQ=60)

                                                                                          AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      >  SRR4302533.5557292/1‑94 (MQ=60)

                                                                                          AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      >  SRR4302533.2617645/1‑94 (MQ=60)

                                                                                          AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      >  SRR4302533.586114/1‑94 (MQ=60)

                                                                                          AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      <  SRR4302533.2675009/94‑1 (MQ=60)

                                                                                           GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     <  SRR4302533.1406103/94‑1 (MQ=60)

                                                                                             G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  SRR4302533.6091995/1‑94 (MQ=60)

                                                                                             G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGGATTCTCCGT‑ACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  SRR4302533.2395594/1‑94 (MQ=60)

TAAGCAGGCATGTTATCCCC‑TTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT‑A‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013625‑4013809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`