Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,023,531	T→C	H318H (CAT→CAC)	yigC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,023,531	0	T	C	99.6%	850.7 / NA	263	H318H (CAT→CAC)	yigC	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (152/110); minor base A (1/0); total (153/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

TGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT  >  NC_000913/4023441‑4023620

tGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTc                                                                                         >  1:2503536/1‑93 (MQ=255)

 ggCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTcc                                                                                        <  1:3352389/93‑1 (MQ=255)

 ggCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTcc                                                                                        >  1:4533186/1‑93 (MQ=255)

   cGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCAc                                                                                      >  2:3476389/1‑93 (MQ=255)

   cGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCAc                                                                                      <  2:3714532/93‑1 (MQ=255)

    gACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCAcc                                                                                     <  1:4226350/93‑1 (MQ=255)

         cacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAc                                                                                >  2:3961260/1‑93 (MQ=255)

         cacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAc                                                                                >  1:6269169/1‑93 (MQ=255)

          acCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAcc                                                                               >  2:3054533/1‑93 (MQ=255)

           ccGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCg                                                                              >  1:3239455/1‑93 (MQ=255)

            cGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCgg                                                                             <  2:3682055/93‑1 (MQ=255)

            cGGTTACTATAATGAAGTAGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCgg                                                                             <  2:5063123/93‑1 (MQ=255)

             ggTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCggg                                                                            <  1:3780354/93‑1 (MQ=255)

             ggTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCggg                                                                            >  1:5995365/1‑93 (MQ=255)

             ggTTACTATAATGAAGTCGATAGTTGCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCTCCTATACCggg                                                                            <  2:1547723/93‑1 (MQ=255)

              gTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGc                                                                           >  2:5779146/1‑93 (MQ=255)

              gTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGc                                                                           >  2:405167/1‑93 (MQ=255)

                tACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGt                                                                         >  1:988407/1‑93 (MQ=255)

                  cTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTcc                                                                       >  1:5918687/1‑93 (MQ=255)

                   tataATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCg                                                                      >  1:2557629/1‑93 (MQ=255)

                   tataATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCg                                                                      >  1:682553/1‑93 (MQ=255)

                    ataATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGc                                                                     >  1:3722602/1‑93 (MQ=255)

                     taATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGcc                                                                    <  2:3820976/93‑1 (MQ=255)

                      aaTGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCa                                                                   <  1:2744546/93‑1 (MQ=255)

                      aaTGAAGTCCATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCa                                                                   <  2:2129664/93‑1 (MQ=255)

                       aTGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAg                                                                  >  1:6150527/1‑93 (MQ=255)

                        tGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGa                                                                 >  1:3150795/1‑93 (MQ=255)

                         gAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGAt                                                                <  2:1457959/93‑1 (MQ=255)

                         gAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGAt                                                                >  2:2953015/1‑93 (MQ=255)

                          aaGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTCCCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATg                                                               <  2:3388145/93‑1 (MQ=255)

                          aaGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATg                                                               >  2:2251973/1‑93 (MQ=255)

                          aaGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATg                                                               >  1:3550777/1‑93 (MQ=255)

                          aaGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATg                                                               >  1:5723399/1‑93 (MQ=255)

                          aaGTCGATAGTTTCCCGGGATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACTACTCCACCTATACCGGTCGTCCGCCAGATg                                                               >  2:2413815/1‑93 (MQ=255)

                           aGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGa                                                              <  2:5154045/93‑1 (MQ=255)

                           aGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGa                                                              >  1:2493641/1‑93 (MQ=255)

                            gTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAg                                                             <  1:4411849/93‑1 (MQ=255)

                            gTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAg                                                             >  2:2191044/1‑93 (MQ=255)

                            gTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAg                                                             >  1:4061720/1‑93 (MQ=255)

                              cGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAggc                                                           >  2:889363/1‑91 (MQ=255)

                              cGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGcc                                                           <  2:5551947/93‑1 (MQ=255)

                                gtAGTTTCCCGGTATTTGCCGTGACGCATGTTCACCAGCGTAAAGATGCAATTTACCACTCCACCAATACCGAACGTCCGCCAGATAAGCCcg                                                         <  2:6305700/92‑1 (MQ=255)

                                aTAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCcg                                                         >  2:5209452/1‑93 (MQ=255)

                                     ttCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTg                                                    >  1:5613117/1‑93 (MQ=255)

                                         cGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTgg                                                >  1:159894/1‑93 (MQ=255)

                                          ggTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTggg                                               >  2:6229263/1‑93 (MQ=255)

                                          ggTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTggg                                               >  1:5323937/1‑93 (MQ=255)

                                          ggTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTggg                                               >  1:4882305/1‑93 (MQ=255)

                                           gTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              >  2:4444526/1‑93 (MQ=255)

                                            tATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtg                                             >  2:675612/1‑93 (MQ=255)

                                              tttACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTc                                           <  2:6110722/93‑1 (MQ=255)

                                                tACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGc                                         >  2:2785153/1‑93 (MQ=255)

                                                 aCCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCa                                        >  1:6024868/1‑93 (MQ=255)

                                                     tGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGa                                    >  1:101613/1‑93 (MQ=255)

                                                       aCGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAc                                  >  2:1254282/1‑93 (MQ=255)

                                                       aCGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAc                                  <  2:1925238/93‑1 (MQ=255)

                                                       aCGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAc                                  >  1:1135016/1‑93 (MQ=255)

                                                       aCGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAc                                  <  2:5025446/93‑1 (MQ=255)

                                                         gCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGa                                >  2:4239530/1‑93 (MQ=255)

                                                         gCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGa                                <  1:5040363/93‑1 (MQ=255)

                                                         gCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGa                                >  2:4860884/1‑93 (MQ=255)

                                                          cATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGaa                               <  2:4226045/93‑1 (MQ=255)

                                                          cATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGaa                               >  2:2741061/1‑93 (MQ=255)

                                                           atatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAg                              >  2:5286677/1‑93 (MQ=255)

                                                           atatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAg                              >  2:431893/1‑93 (MQ=255)

                                                           atatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAg                              <  1:1431471/93‑1 (MQ=255)

                                                            tatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGGGTCGCACTGAACGAAgt                             >  2:3200470/1‑93 (MQ=255)

                                                             atTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgtg                            <  2:3231025/93‑1 (MQ=255)

                                                              ttACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgtgt                           >  1:2941806/1‑93 (MQ=255)

                                                               tACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGtt                          <  1:2151024/93‑1 (MQ=255)

                                                                aCCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGttt                         <  1:5287635/93‑1 (MQ=255)

                                                                  ccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       >  2:473001/1‑93 (MQ=255)

                                                                   cAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgtg                      >  1:4293307/1‑93 (MQ=255)

                                                                   cAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgtg                      >  1:277245/1‑93 (MQ=255)

                                                                       gTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCgt                  >  1:5223529/1‑92 (MQ=255)

                                                                       gTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGa                  <  1:5198801/93‑1 (MQ=255)

                                                                        tGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 <  1:4394365/93‑1 (MQ=255)

                                                                        tGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 <  1:1988247/93‑1 (MQ=255)

                                                                        tGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 >  1:1639522/1‑93 (MQ=255)

                                                                        tGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 >  1:5526719/1‑93 (MQ=255)

                                                                        tGAAGATGAGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 >  1:4885621/1‑93 (MQ=255)

                                                                         gAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAtt                >  2:947253/1‑93 (MQ=255)

                                                                         gAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAtt                <  1:1093102/93‑1 (MQ=255)

                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               >  1:861965/1‑93 (MQ=255)

                                                                           aGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCt              <  2:1513238/93‑1 (MQ=255)

                                                                           aGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCt              <  1:54238/93‑1 (MQ=255)

                                                                            gATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTg             >  2:3943681/1‑93 (MQ=255)

                                                                            gATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTg             <  1:3865418/93‑1 (MQ=255)

                                                                             aTGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGc            <  2:1159761/93‑1 (MQ=255)

                                                                             aTGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGc            <  1:2360444/93‑1 (MQ=255)

                                                                                 gATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaaaa        >  1:1935067/1‑93 (MQ=255)

                                                                                  aTTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGGCGATTCTGCaaaaa       >  1:1545348/1‑93 (MQ=255)

                                                                                   tttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAc      <  2:5641979/93‑1 (MQ=255)

                                                                                   tttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAc      <  2:6074222/93‑1 (MQ=255)

                                                                                    ttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACa     >  2:3416712/1‑93 (MQ=255)

                                                                                     tACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAg    <  2:3548154/93‑1 (MQ=255)

                                                                                      aCCACTCCACTTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGt   >  2:3858682/1‑93 (MQ=255)

                                                                                      aCCACTCCACCTATACCGGTCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAATACAGt   >  1:3811771/1‑93 (MQ=255)

                                                                                       ccACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGtt  <  1:3454866/93‑1 (MQ=255)

                                                                                       ccACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGtt  <  2:1005220/93‑1 (MQ=255)

TGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT  >  NC_000913/4023441‑4023620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  NC_000913/4023438‑4023624

GTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAC                                                                                               <  SRR4302533.3683416/94‑1 (MQ=60)

GTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAC                                                                                               >  SRR4302533.4557986/1‑94 (MQ=60)

 TATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACT                                                                                              <  SRR4302533.3277065/94‑1 (MQ=60)

  ATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTC                                                                                             >  SRR4302533.2518970/1‑94 (MQ=60)

   TGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCC                                                                                            >  SRR4302533.4560634/1‑94 (MQ=60)

    GGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCA                                                                                           <  SRR4302533.3373098/94‑1 (MQ=60)

     GCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCAC                                                                                          >  SRR4302533.1900039/1‑94 (MQ=60)

      CGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACC                                                                                         >  SRR4302533.348311/1‑94 (MQ=60)

       GACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCT                                                                                        <  SRR4302533.4251909/94‑1 (MQ=60)

       GACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCT                                                                                        >  SRR4302533.4436060/1‑94 (MQ=60)

        ACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTA                                                                                       >  SRR4302533.994077/1‑94 (MQ=60)

          CACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATA                                                                                     <  SRR4302533.5238504/94‑1 (MQ=60)

           ACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAC                                                                                    >  SRR4302533.6306627/1‑94 (MQ=60)

            CACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACC                                                                                   >  SRR4302533.2830865/1‑94 (MQ=60)

             ACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCG                                                                                  >  SRR4302533.3259418/1‑94 (MQ=60)

              CCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGG                                                                                 >  SRR4302533.823685/1‑94 (MQ=60)

               CGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGG                                                                                >  SRR4302533.6031677/1‑94 (MQ=60)

               CGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGG                                                                                <  SRR4302533.3649930/94‑1 (MQ=60)

                GGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGC                                                                               <  SRR4302533.3803514/94‑1 (MQ=60)

                GGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGC                                                                               >  SRR4302533.965093/1‑94 (MQ=60)

                  TTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGT                                                                             >  SRR4302533.994191/1‑94 (MQ=60)

                    ACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCC                                                                           >  SRR4302533.5954457/1‑94 (MQ=60)

                    ACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCC                                                                           <  SRR4302533.2570965/94‑1 (MQ=60)

                    ACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCC                                                                           <  SRR4302533.1983339/94‑1 (MQ=60)

                     CTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCG                                                                          <  SRR4302533.4465174/94‑1 (MQ=60)

                     CTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCG                                                                          >  SRR4302533.4257762/1‑94 (MQ=60)

                     CTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCG                                                                          >  SRR4302533.2573354/1‑94 (MQ=60)

                     CTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCG                                                                          <  SRR4302533.4384376/94‑1 (MQ=60)

                     CTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCG                                                                          >  SRR4302533.686590/1‑94 (MQ=60)

                     CTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCG                                                                          <  SRR4302533.3292514/94‑1 (MQ=60)

                      TATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGC                                                                         >  SRR4302533.6305652/1‑94 (MQ=60)

                      TATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGC                                                                         >  SRR4302533.3745444/1‑94 (MQ=60)

                      TATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGC                                                                         >  SRR4302533.5414733/1‑94 (MQ=60)

                        TAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCA                                                                       >  SRR4302533.6162270/1‑94 (MQ=60)

                         AATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAG                                                                      <  SRR4302533.2761370/94‑1 (MQ=60)

                         AATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAG                                                                      >  SRR4302533.6187548/1‑94 (MQ=60)

                          ATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGA                                                                     >  SRR4302533.3170169/1‑94 (MQ=60)

                            GAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATG                                                                   >  SRR4302533.3572651/1‑94 (MQ=60)

                            GAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATG                                                                   <  SRR4302533.1003307/94‑1 (MQ=60)

                            GAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATG                                                                   >  SRR4302533.5757952/1‑94 (MQ=60)

                             AAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGA                                                                  >  SRR4302533.2509022/1‑94 (MQ=60)

                              AGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAG                                                                 <  SRR4302533.3885650/94‑1 (MQ=60)

                              AGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAG                                                                 <  SRR4302533.3071967/94‑1 (MQ=60)

                              AGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAG                                                                 >  SRR4302533.4086445/1‑94 (MQ=60)

                               GTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGC                                                                <  SRR4302533.4438493/94‑1 (MQ=60)

                               GTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGC                                                                <  SRR4302533.6257151/94‑1 (MQ=60)

                                 CGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCC                                                              >  SRR4302533.4927608/1‑94 (MQ=60)

                                  GATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCG                                                             <  SRR4302533.5845601/94‑1 (MQ=60)

                                   ATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGC                                                            >  SRR4302533.5981490/1‑94 (MQ=60)

                                      GTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGT                                                         <  SRR4302533.453277/94‑1 (MQ=60)

                                       TTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTG                                                        <  SRR4302533.522678/94‑1 (MQ=60)

                                       TTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTG                                                        <  SRR4302533.435511/94‑1 (MQ=60)

                                       TTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTG                                                        >  SRR4302533.5647044/1‑94 (MQ=60)

                                       TTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTG                                                        <  SRR4302533.2420392/94‑1 (MQ=60)

                                          CCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTG                                                     >  SRR4302533.1552904/1‑94 (MQ=60)

                                          CCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTG                                                     >  SRR4302533.4929305/1‑94 (MQ=60)

                                           CCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGG                                                    >  SRR4302533.160916/1‑94 (MQ=60)

                                            CGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGG                                                   >  SRR4302533.404119/1‑94 (MQ=60)

                                            CGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGG                                                   <  SRR4302533.3960050/94‑1 (MQ=60)

                                            CGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGG                                                   >  SRR4302533.4911882/1‑94 (MQ=60)

                                            CGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGG                                                   >  SRR4302533.5355844/1‑94 (MQ=60)

                                             GGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGT                                                  >  SRR4302533.2304159/1‑94 (MQ=60)

                                             GGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGT                                                  >  SRR4302533.6056760/1‑94 (MQ=60)

                                              GTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTG                                                 <  SRR4302533.2876229/94‑1 (MQ=60)

                                              GTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTG                                                 <  SRR4302533.2518882/94‑1 (MQ=60)

                                              GTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTG                                                 >  SRR4302533.6321341/1‑94 (MQ=60)

                                                ATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTC                                               <  SRR4302533.3464562/94‑1 (MQ=60)

                                                ATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTC                                               <  SRR4302533.2143732/94‑1 (MQ=60)

                                                ATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTC                                               >  SRR4302533.4213750/1‑94 (MQ=60)

                                                   TACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCA                                            >  SRR4302533.598587/1‑94 (MQ=60)

                                                   TACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCA                                            >  SRR4302533.6061377/1‑94 (MQ=60)

                                                     CCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACT                                          <  SRR4302533.2520219/94‑1 (MQ=60)

                                                     CCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACT                                          >  SRR4302533.5934241/1‑94 (MQ=60)

                                                       GTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGA                                        >  SRR4302533.102243/1‑94 (MQ=60)

                                                        TGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAA                                       >  SRR4302533.3830917/1‑94 (MQ=60)

                                                        TGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAA                                       >  SRR4302533.2768543/1‑94 (MQ=60)

                                                        TGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAA                                       >  SRR4302533.3414315/1‑94 (MQ=60)

                                                         GACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAC                                      >  SRR4302533.1141766/1‑94 (MQ=60)

                                                         GACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAC                                      <  SRR4302533.1572558/94‑1 (MQ=60)

                                                          ACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACG                                     >  SRR4302533.3182659/1‑94 (MQ=60)

                                                            GCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAA                                   >  SRR4302533.1039286/1‑94 (MQ=60)

                                                            GCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAA                                   <  SRR4302533.5070874/94‑1 (MQ=60)

                                                             CATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAG                                  >  SRR4302533.2677639/1‑94 (MQ=60)

                                                              ATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGT                                 <  SRR4302533.1440121/94‑1 (MQ=60)

                                                              ATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGT                                 >  SRR4302533.5297628/1‑94 (MQ=60)

                                                              ATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGT                                 >  SRR4302533.4784575/1‑94 (MQ=60)

                                                                ATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGTTGGGTGTCGCACTGAACGAAGTGT                               >  SRR4302533.5341752/1‑94 (MQ=60)

                                                                ATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGT                               >  SRR4302533.3751966/1‑94 (MQ=60)

                                                                ATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGT                               >  SRR4302533.2959771/1‑94 (MQ=60)

                                                                 TTACCCAGCGTGAAGATGCGATTTACCACCCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTT                              <  SRR4302533.5598756/94‑1 (MQ=60)

                                                                  TACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTT                             >  SRR4302533.5466627/1‑94 (MQ=60)

                                                                  TACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTT                             <  SRR4302533.2164270/94‑1 (MQ=60)

                                                                   ACCCAGCGTGAAGATGCGATTTACCACTCCACCTATCCCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTG                            <  SRR4302533.1215496/94‑1 (MQ=60)

                                                                   ACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTG                            <  SRR4302533.5319303/94‑1 (MQ=60)

                                                                    CCCAGCGTGAAGATGCTATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGT                           <  SRR4302533.1113173/94‑1 (MQ=60)

                                                                    CCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGT                           >  SRR4302533.4318427/1‑94 (MQ=60)

                                                                    CCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGT                           >  SRR4302533.3799938/1‑94 (MQ=60)

                                                                     CCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTG                          >  SRR4302533.278938/1‑94 (MQ=60)

                                                                     CCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTG                          >  SRR4302533.4319128/1‑94 (MQ=60)

                                                                       AGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCC                        >  SRR4302533.818656/1‑94 (MQ=60)

                                                                       AGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCC                        >  SRR4302533.1693001/1‑94 (MQ=60)

                                                                       AGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCC                        <  SRR4302533.5563456/94‑1 (MQ=60)

                                                                        GCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCG                       >  SRR4302533.6077028/1‑94 (MQ=60)

                                                                         CGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGT                      >  SRR4302533.5254955/1‑94 (MQ=60)

                                                                          GTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAT                     >  SRR4302533.4915217/1‑94 (MQ=60)

                                                                          GTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAT                     >  SRR4302533.5560084/1‑94 (MQ=60)

                                                                          GTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAT                     >  SRR4302533.1649450/1‑94 (MQ=60)

                                                                          GTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAT                     <  SRR4302533.5230149/94‑1 (MQ=60)

                                                                          GTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAT                     >  SRR4302533.1649451/1‑94 (MQ=60)

                                                                          GTGAAGATGAGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAT                     >  SRR4302533.4915219/1‑94 (MQ=60)

                                                                           TGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATT                    <  SRR4302533.2000410/94‑1 (MQ=60)

                                                                           TGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATT                    <  SRR4302533.4420897/94‑1 (MQ=60)

                                                                            GAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTC                   <  SRR4302533.1099569/94‑1 (MQ=60)

                                                                            GAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTC                   >  SRR4302533.867016/1‑94 (MQ=60)

                                                                            GAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTC                   >  SRR4302533.5336691/1‑94 (MQ=60)

                                                                             AAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCT                  >  SRR4302533.3878942/1‑94 (MQ=60)

                                                                             AAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCT                  <  SRR4302533.3261349/94‑1 (MQ=60)

                                                                             AAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCT                  <  SRR4302533.5281143/94‑1 (MQ=60)

                                                                              AGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTG                 <  SRR4302533.54605/94‑1 (MQ=60)

                                                                               GATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGC                >  SRR4302533.2173372/1‑94 (MQ=60)

                                                                               GATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGC                <  SRR4302533.3889015/94‑1 (MQ=60)

                                                                                ATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCA               <  SRR4302533.2375032/94‑1 (MQ=60)

                                                                                ATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCA               <  SRR4302533.1579089/94‑1 (MQ=60)

                                                                                   CGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAA            >  SRR4302533.1946871/1‑94 (MQ=60)

                                                                                   CGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAA            <  SRR4302533.5091935/94‑1 (MQ=60)

                                                                                   CGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAA            >  SRR4302533.4777118/1‑94 (MQ=60)

                                                                                    GATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGGCGATTCTGCAAAAA           >  SRR4302533.1554658/1‑94 (MQ=60)

                                                                                     ATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCACAAAC          >  SRR4302533.2678022/1‑94 (MQ=60)

                                                                                     ATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAC          >  SRR4302533.5655181/1‑94 (MQ=60)

                                                                                     ATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAC          >  SRR4302533.3723739/1‑94 (MQ=60)

                                                                                      TTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACA         <  SRR4302533.1998800/94‑1 (MQ=60)

                                                                                       TTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAG        >  SRR4302533.5548336/1‑94 (MQ=60)

                                                                                        TACCACTCCACCTATACCGGTCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAATACAGT       >  SRR4302533.3835081/1‑94 (MQ=60)

                                                                                        TACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGT       <  SRR4302533.303581/94‑1 (MQ=60)

                                                                                         ACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT      >  SRR4302533.2477509/1‑94 (MQ=60)

                                                                                          CCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTC     <  SRR4302533.1486194/94‑1 (MQ=60)

                                                                                          CCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTC     <  SRR4302533.3476191/94‑1 (MQ=60)

                                                                                          CCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTC     <  SRR4302533.1106580/94‑1 (MQ=60)

                                                                                           CACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTTCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCC    >  SRR4302533.3755501/1‑94 (MQ=60)

                                                                                           CACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCC    >  SRR4302533.3185490/1‑94 (MQ=60)

                                                                                           CACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCC    >  SRR4302533.5072424/1‑94 (MQ=60)

                                                                                            ACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCC   >  SRR4302533.5389246/1‑94 (MQ=60)

                                                                                             CTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  <  SRR4302533.1229025/94‑1 (MQ=60)

                                                                                             CTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  SRR4302533.764097/1‑94 (MQ=60)

                                                                                             CTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  SRR4302533.3572032/1‑94 (MQ=60)

                                                                                             CTCCACCTATACCGGGCGTCCGCAAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  <  SRR4302533.655636/94‑1 (MQ=60)

GTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  NC_000913/4023438‑4023624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`