Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,049,140	A→G	I72V (ATT→GTT)	yihI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,049,140	0	A	G	100.0%	796.8 / NA	241	I72V (ATT→GTT)	yihI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (116/125); total (116/125)

Only 100 of 246 total aligned reads displayed.

CCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA  >  NC_000913/4049050‑4049229

 cGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTc                                                                                        <  2:3455522/93‑1 (MQ=255)

 cGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTc                                                                                        <  2:2976247/93‑1 (MQ=255)

  gggCAGCCGCGCAGCGGGCGGAAGCACCACGTCAGGCAGCAAAGGCCCGAACGCACCAAAAGATCCACGTATTGGAAGTAAAACCCCTGTTcc                                                                                       >  2:1181851/1‑93 (MQ=255)

  gggCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTcc                                                                                       >  2:6280900/1‑93 (MQ=255)

   ggCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCa                                                                                      <  2:3887471/93‑1 (MQ=255)

   ggCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCa                                                                                      >  1:1474717/1‑93 (MQ=255)

   ggCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCa                                                                                      >  1:2547195/1‑93 (MQ=255)

     cAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCAtt                                                                                    <  1:6239898/93‑1 (MQ=255)

     cAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCAtt                                                                                    <  2:1097307/93‑1 (MQ=255)

      aGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTg                                                                                   <  1:4392508/93‑1 (MQ=255)

      aGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTg                                                                                   <  2:2209742/93‑1 (MQ=255)

       gCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTga                                                                                  >  1:5460855/1‑92 (MQ=255)

       gCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTga                                                                                  >  1:2299202/1‑92 (MQ=255)

       gCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTga                                                                                  >  2:3607772/1‑92 (MQ=255)

        ccGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTgag                                                                                 <  1:5649495/93‑3 (MQ=255)

         cgcgCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGc                                                                                <  1:5247099/93‑1 (MQ=255)

           cgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGt                                                                              <  1:2603540/93‑1 (MQ=255)

            gcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTg                                                                             <  2:1954733/93‑1 (MQ=255)

            gcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTg                                                                             >  2:3106506/1‑93 (MQ=255)

            gcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTg                                                                             >  2:597362/1‑93 (MQ=255)

              aGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                           >  2:2802215/1‑93 (MQ=255)

               gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                          >  1:1111510/1‑93 (MQ=255)

               gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                          >  1:3488727/1‑93 (MQ=255)

                 gggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                        >  1:4840147/1‑93 (MQ=255)

                   gcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaa                                                                      <  1:3686890/93‑1 (MQ=255)

                    cggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGTCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaa                                                                     >  1:6137992/1‑93 (MQ=255)

                      gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGCTCCACGTATTGGCAGTAAAACACCTGTTCCATTGAGCGTGACTGAAAAAg                                                                   >  2:1609992/1‑93 (MQ=255)

                      gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAg                                                                   >  1:2020818/1‑93 (MQ=255)

                      gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAg                                                                   >  1:5687817/1‑93 (MQ=255)

                        aaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTc                                                                 <  1:2117780/93‑1 (MQ=255)

                        aaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTc                                                                 >  2:5843630/1‑93 (MQ=255)

                         acacCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCa                                                                >  1:310659/1‑93 (MQ=255)

                           accacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAcc                                                              <  1:185704/93‑1 (MQ=255)

                            ccacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCa                                                             <  1:3919177/93‑1 (MQ=255)

                              acGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                           >  1:226472/1‑93 (MQ=255)

                               cGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAc                                                          <  1:4651727/93‑1 (MQ=255)

                                gTCAGGCAGCAAAGGCCAGAACGCGCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                         <  2:4626690/93‑1 (MQ=255)

                                gTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                         <  1:4018713/93‑1 (MQ=255)

                                gTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                         >  2:4869217/1‑93 (MQ=255)

                                 tCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAg                                                        <  2:773510/93‑1 (MQ=255)

                                  cAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGc                                                       >  1:1359512/1‑93 (MQ=255)

                                  cAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGc                                                       <  1:2199750/93‑1 (MQ=255)

                                  cAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGc                                                       >  1:5506902/1‑93 (MQ=255)

                                    ggCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGcac                                                     <  2:4549194/93‑1 (MQ=255)

                                     gcagcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGcaca                                                    <  2:4380912/93‑1 (MQ=255)

                                      cagcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaa                                                   <  2:4477049/93‑1 (MQ=255)

                                      cagcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaa                                                   <  2:5096217/93‑1 (MQ=255)

                                      cagcaAAGCCCAGAACGCACCAAAAGACCCTCGTATTGACAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaa                                                   <  1:5254392/93‑1 (MQ=255)

                                        gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                 <  2:4134318/93‑1 (MQ=255)

                                        gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                 >  1:237335/1‑93 (MQ=255)

                                        gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                 >  1:2871996/1‑93 (MQ=255)

                                        gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCCGTTCCATTGAGCGTGAATGAAAAAGTCACCAAACAGCACAAAc                                                 >  1:5927333/1‑93 (MQ=255)

                                            aGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                                             <  1:2650295/93‑1 (MQ=255)

                                               ccAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagag                                          <  2:5219592/93‑1 (MQ=255)

                                               ccAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagag                                          >  1:1736730/1‑93 (MQ=255)

                                               ccAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagag                                          <  2:5706120/93‑1 (MQ=255)

                                                cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                         <  1:3760806/93‑1 (MQ=255)

                                                 aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTg                                        >  1:5238383/1‑93 (MQ=255)

                                                 aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACAGAAGAGTg                                        >  2:4520068/1‑93 (MQ=255)

                                                  gAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTga                                       >  2:4932116/1‑93 (MQ=255)

                                                   aaCGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgag                                      >  1:4092321/1‑93 (MQ=255)

                                                    aCGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgaga                                     >  1:3989499/1‑93 (MQ=255)

                                                      gCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGaaa                                   >  2:2419759/1‑93 (MQ=255)

                                                      gCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGaaa                                   <  2:332697/93‑1 (MQ=255)

                                                          cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                               <  1:1791345/93‑1 (MQ=255)

                                                           aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                              >  2:4792144/1‑93 (MQ=255)

                                                           aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                              <  1:143988/93‑1 (MQ=255)

                                                            aaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATg                             <  1:907610/93‑1 (MQ=255)

                                                            aaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATg                             <  1:1675461/93‑1 (MQ=255)

                                                             aaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGc                            <  1:3813652/93‑1 (MQ=255)

                                                             aaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGc                            <  2:5003951/93‑1 (MQ=255)

                                                               gATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCtt                          >  1:6020858/1‑93 (MQ=255)

                                                                aTCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCttt                         <  1:4234706/93‑1 (MQ=255)

                                                                aTCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCttt                         >  2:154000/1‑93 (MQ=255)

                                                                 tCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTc                        >  1:1558466/1‑93 (MQ=255)

                                                                  ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                       >  1:5839028/1‑93 (MQ=255)

                                                                   cACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAc                      <  2:5374601/93‑1 (MQ=255)

                                                                   cACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAc                      >  2:3032301/1‑93 (MQ=255)

                                                                     cGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCg                    >  1:2440679/1‑93 (MQ=255)

                                                                       tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                  >  2:2526028/1‑93 (MQ=255)

                                                                         ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                <  2:4291859/93‑1 (MQ=255)

                                                                         ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                <  1:6075281/93‑1 (MQ=255)

                                                                            gCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCgg             >  2:2312448/1‑93 (MQ=255)

                                                                            gCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCgg             <  2:5762677/93‑1 (MQ=255)

                                                                              aGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAg           <  2:833312/93‑1 (MQ=255)

                                                                                tAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGtt         >  2:4107834/1‑93 (MQ=255)

                                                                                 aaaaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTg        <  2:370773/93‑1 (MQ=255)

                                                                                 aaaaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTg        <  2:5291919/93‑1 (MQ=255)

                                                                                    aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCTAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa     <  1:3389559/93‑1 (MQ=255)

                                                                                    aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa     >  2:4124651/1‑93 (MQ=255)

                                                                                    aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa     >  2:2401663/1‑93 (MQ=255)

                                                                                    aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa     <  2:6199983/93‑1 (MQ=255)

                                                                                    aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa     <  1:105696/93‑1 (MQ=255)

                                                                                    aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa     <  1:6167053/93‑1 (MQ=255)

                                                                                    aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa     <  1:2908340/93‑1 (MQ=255)

                                                                                     ccccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAt    >  2:1907361/1‑93 (MQ=255)

                                                                                     ccccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTCTCACCGCAGGCGGAGTTGGAAt    >  1:959447/1‑93 (MQ=255)

                                                                                      cccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAtt   >  1:1250063/1‑93 (MQ=255)

                                                                                       ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa  >  2:3572810/1‑93 (MQ=255)

                                                                                       ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa  >  2:5511144/1‑93 (MQ=255)

CCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA  >  NC_000913/4049050‑4049229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG  >  NC_000913/4049047‑4049232

GCGCCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTG                                                                                              <  SRR4302533.2355972/94‑1 (MQ=60)

GCGCCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTG                                                                                              <  SRR4302533.4124644/94‑1 (MQ=60)

GCGCCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTG                                                                                              <  SRR4302533.6329121/94‑1 (MQ=60)

     GGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCA                                                                                         >  SRR4302533.2562871/1‑94 (MQ=60)

     GGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCA                                                                                         >  SRR4302533.1483632/1‑94 (MQ=60)

        CAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTG                                                                                      <  SRR4302533.6277262/94‑1 (MQ=60)

         AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                     <  SRR4302533.1167727/94‑1 (MQ=60)

         AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                     >  SRR4302533.2313457/1‑94 (MQ=60)

         AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                     <  SRR4302533.4419024/94‑1 (MQ=60)

         AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                     >  SRR4302533.5493755/1‑94 (MQ=60)

          GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                    <  SRR4302533.1418228/94‑1 (MQ=60)

          GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                    <  SRR4302533.5417995/94‑1 (MQ=60)

           CCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGC                                                                                   <  SRR4302533.5683646/94‑1 (MQ=60)

            CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCG                                                                                  <  SRR4302533.5278647/94‑1 (MQ=60)

              CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                <  SRR4302533.1835696/94‑1 (MQ=60)

              CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                <  SRR4302533.2619534/94‑1 (MQ=60)

               GCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGA                                                                               <  SRR4302533.4326114/94‑1 (MQ=60)

                CAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAC                                                                              >  SRR4302533.231343/1‑94 (MQ=60)

                 AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                             >  SRR4302533.3510242/1‑94 (MQ=60)

                 AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                             <  SRR4302533.5537467/94‑1 (MQ=60)

                 AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                             >  SRR4302533.1118106/1‑94 (MQ=60)

                   CGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGGGCGTGACTGA                                                                           >  SRR4302533.4704310/1‑94 (MQ=60)

                   CGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGA                                                                           >  SRR4302533.4869482/1‑94 (MQ=60)

                    GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                          <  SRR4302533.1928473/94‑1 (MQ=60)

                      GCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGTCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAA                                                                        >  SRR4302533.6174967/1‑94 (MQ=60)

                      GCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAA                                                                        <  SRR4302533.3709551/94‑1 (MQ=60)

                       CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                       >  SRR4302533.423650/1‑94 (MQ=60)

                       CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                       <  SRR4302533.4120422/94‑1 (MQ=60)

                        GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                      >  SRR4302533.2033263/1‑94 (MQ=60)

                        GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                      <  SRR4302533.3929220/94‑1 (MQ=60)

                        GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                      <  SRR4302533.1357410/94‑1 (MQ=60)

                        GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                      >  SRR4302533.5722171/1‑94 (MQ=60)

                         GAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGT                                                                     <  SRR4302533.5823786/94‑1 (MQ=60)

                          AAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTC                                                                    <  SRR4302533.3201568/94‑1 (MQ=60)

                           AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                   <  SRR4302533.2130842/94‑1 (MQ=60)

                           AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                   >  SRR4302533.3975087/1‑94 (MQ=60)

                           AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                   >  SRR4302533.312561/1‑94 (MQ=60)

                           AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                   >  SRR4302533.5182543/1‑94 (MQ=60)

                            ACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAC                                                                  <  SRR4302533.6178690/94‑1 (MQ=60)

                             CACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTCGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAGAGTCACC                                                                 >  SRR4302533.527580/1‑94 (MQ=60)

                              ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                >  SRR4302533.2270468/1‑94 (MQ=60)

                              ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                >  SRR4302533.6053399/1‑94 (MQ=60)

                              ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                <  SRR4302533.2411169/94‑1 (MQ=60)

                              ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                <  SRR4302533.186860/94‑1 (MQ=60)

                               CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                               <  SRR4302533.3943111/94‑1 (MQ=60)

                               CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                               <  SRR4302533.3465423/94‑1 (MQ=60)

                                CACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAA                                                              >  SRR4302533.227883/1‑94 (MQ=60)

                                CACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAA                                                              >  SRR4302533.442458/1‑94 (MQ=60)

                                 ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                             <  SRR4302533.2490349/94‑1 (MQ=60)

                                  CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                            <  SRR4302533.4679914/94‑1 (MQ=60)

                                  CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                            <  SRR4302533.2623881/94‑1 (MQ=60)

                                  CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                            >  SRR4302533.2083177/1‑94 (MQ=60)

                                  CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                            >  SRR4302533.2247474/1‑94 (MQ=60)

                                  CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                            <  SRR4302533.6141339/94‑1 (MQ=60)

                                   GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                           <  SRR4302533.4043184/94‑1 (MQ=60)

                                   GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                           <  SRR4302533.6261347/94‑1 (MQ=60)

                                    TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                          >  SRR4302533.5540133/1‑94 (MQ=60)

                                    TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                          >  SRR4302533.1367683/1‑94 (MQ=60)

                                     CAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCA                                                         <  SRR4302533.2326057/94‑1 (MQ=60)

                                     CAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCA                                                         <  SRR4302533.2213301/94‑1 (MQ=60)

                                         CAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAA                                                     <  SRR4302533.5285957/94‑1 (MQ=60)

                                         CAGCAAAGCCCAGAACGCACCAAAAGACCCTCGTATTGACAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAA                                                     <  SRR4302533.5285958/94‑1 (MQ=60)

                                          AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                    >  SRR4302533.6231967/1‑94 (MQ=60)

                                          AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                    >  SRR4302533.2889667/1‑94 (MQ=60)

                                          AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                    >  SRR4302533.6102772/1‑94 (MQ=60)

                                          AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                    <  SRR4302533.305580/94‑1 (MQ=60)

                                          AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                    >  SRR4302533.238805/1‑94 (MQ=60)

                                          AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCCGTTCCATTGAGCGTGAATGAAAAAGTCACCAAACAGCACAAAC                                                    >  SRR4302533.5963166/1‑94 (MQ=60)

                                           GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                   <  SRR4302533.940161/94‑1 (MQ=60)

                                           GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                   >  SRR4302533.5744455/1‑94 (MQ=60)

                                            CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                  <  SRR4302533.5267909/94‑1 (MQ=60)

                                             AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                 >  SRR4302533.3782382/1‑94 (MQ=60)

                                             AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                 >  SRR4302533.2663272/1‑94 (MQ=60)

                                               AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                               <  SRR4302533.2666575/94‑1 (MQ=60)

                                               AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                               <  SRR4302533.728715/94‑1 (MQ=60)

                                                GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                              <  SRR4302533.5413445/94‑1 (MQ=60)

                                                 GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                             >  SRR4302533.1747269/1‑94 (MQ=60)

                                                 GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                             >  SRR4302533.2700290/1‑94 (MQ=60)

                                                  CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                            >  SRR4302533.3627034/1‑94 (MQ=60)

                                                   CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                           <  SRR4302533.3783856/94‑1 (MQ=60)

                                                   CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                           >  SRR4302533.5269879/1‑94 (MQ=60)

                                                    AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                          <  SRR4302533.1606870/94‑1 (MQ=60)

                                                     GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                         >  SRR4302533.4117231/1‑94 (MQ=60)

                                                      AACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGA                                        >  SRR4302533.4013798/1‑94 (MQ=60)

                                                       ACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAA                                       >  SRR4302533.2906201/1‑94 (MQ=60)

                                                         GCACCAAAAGATCCACGTATTGTCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                     <  SRR4302533.1778601/94‑1 (MQ=60)

                                                         GCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                     >  SRR4302533.2572281/1‑94 (MQ=60)

                                                          CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                    >  SRR4302533.5983012/1‑94 (MQ=60)

                                                           ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                   >  SRR4302533.4016312/1‑94 (MQ=60)

                                                             CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                 <  SRR4302533.1802237/94‑1 (MQ=60)

                                                             CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                 <  SRR4302533.5878189/94‑1 (MQ=60)

                                                             CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                 <  SRR4302533.4026474/94‑1 (MQ=60)

                                                              AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                <  SRR4302533.2018388/94‑1 (MQ=60)

                                                              AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                <  SRR4302533.144911/94‑1 (MQ=60)

                                                               AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                               <  SRR4302533.1685607/94‑1 (MQ=60)

                                                               AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                               <  SRR4302533.912932/94‑1 (MQ=60)

                                                                AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                              <  SRR4302533.5699047/94‑1 (MQ=60)

                                                                AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                              <  SRR4302533.3836974/94‑1 (MQ=60)

                                                                 AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                             >  SRR4302533.6057343/1‑94 (MQ=60)

                                                                 AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                             <  SRR4302533.1589464/94‑1 (MQ=60)

                                                                 AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                             >  SRR4302533.3763687/1‑94 (MQ=60)

                                                                   ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                           >  SRR4302533.1733982/1‑94 (MQ=60)

                                                                   ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                           <  SRR4302533.4260294/94‑1 (MQ=60)

                                                                   ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                           >  SRR4302533.1567851/1‑94 (MQ=60)

                                                                    TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                          >  SRR4302533.5874329/1‑94 (MQ=60)

                                                                     CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                         >  SRR4302533.3272894/1‑94 (MQ=60)

                                                                      CACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACC                        >  SRR4302533.6133861/1‑94 (MQ=60)

                                                                       ACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCG                       >  SRR4302533.2455748/1‑94 (MQ=60)

                                                                        CGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGC                      <  SRR4302533.3812115/94‑1 (MQ=60)

                                                                        CGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGC                      >  SRR4302533.1283794/1‑94 (MQ=60)

                                                                         GTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCA                     >  SRR4302533.1459381/1‑94 (MQ=60)

                                                                            TTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGC                  <  SRR4302533.6112029/94‑1 (MQ=60)

                                                                              GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                <  SRR4302533.2911222/94‑1 (MQ=60)

                                                                               GCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGA               >  SRR4302533.2165674/1‑94 (MQ=60)

                                                                                 AGTAAAGCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT             <  SRR4302533.4057796/94‑1 (MQ=60)

                                                                                 AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT             >  SRR4302533.1748181/1‑94 (MQ=60)

                                                                                  GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT            <  SRR4302533.235584/94‑1 (MQ=60)

                                                                                  GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT            >  SRR4302533.5669955/1‑94 (MQ=60)

                                                                                   TAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTG           >  SRR4302533.1449139/1‑94 (MQ=60)

                                                                                    AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG          <  SRR4302533.3839386/94‑1 (MQ=60)

                                                                                     AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA         <  SRR4302533.2547715/94‑1 (MQ=60)

                                                                                     AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA         <  SRR4302533.1522313/94‑1 (MQ=60)

                                                                                     AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA         >  SRR4302533.4562907/1‑94 (MQ=60)

                                                                                       ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCTAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT       <  SRR4302533.3410491/94‑1 (MQ=60)

                                                                                       ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT       <  SRR4302533.6204116/94‑1 (MQ=60)

                                                                                       ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT       <  SRR4302533.5653081/94‑1 (MQ=60)

                                                                                       ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT       <  SRR4302533.2926194/94‑1 (MQ=60)

                                                                                       ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT       <  SRR4302533.106355/94‑1 (MQ=60)

                                                                                       ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTCTCACCGCAGGCGGAGTTGGAAT       >  SRR4302533.965087/1‑94 (MQ=60)

                                                                                       ACCCCTCTTCCATTGAGCGTGACTGAAAAAGCCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT       <  SRR4302533.1110365/94‑1 (MQ=60)

                                                                                        CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT      >  SRR4302533.1257529/1‑94 (MQ=60)

                                                                                           CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT   <  SRR4302533.5072980/94‑1 (MQ=60)

                                                                                           CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT   <  SRR4302533.4896381/94‑1 (MQ=60)

                                                                                           CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT   <  SRR4302533.2252733/94‑1 (MQ=60)

                                                                                            TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG  <  SRR4302533.5580500/94‑1 (MQ=60)

                                                                                            TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG  >  SRR4302533.5334265/1‑94 (MQ=60)

                                                                                            TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG  >  SRR4302533.4530823/1‑94 (MQ=60)

GCGCCGGGCAGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG  >  NC_000913/4049047‑4049232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`