Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,049,149 G→A G75S (GGC→AGC)  yihI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,049,1490GA99.8% 1997.4 / ‑5.9 540G75S (GGC→AGC) yihIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (253/285);  total (254/286)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 548 total aligned reads displayed.

CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238
                                                                                          |                                                                                         
 gcgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCg                                                                                        <  1:6160382/93‑1 (MQ=255)
 gcgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCg                                                                                        <  1:6312964/93‑1 (MQ=255)
 gcgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCg                                                                                        <  1:2507558/93‑1 (MQ=255)
  cgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGt                                                                                       <  2:6556853/93‑1 (MQ=255)
    cAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGa                                                                                     <  1:4359190/93‑1 (MQ=255)
    cAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGa                                                                                     <  1:4069561/93‑1 (MQ=255)
    cAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGa                                                                                     >  1:8574368/1‑93 (MQ=255)
     aGCGGGCGGAAACACCACGTTAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                                    <  1:7772999/93‑1 (MQ=255)
     aGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                                    <  1:1753731/93‑1 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                                   >  1:1150013/1‑93 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                                   >  2:6043687/1‑93 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                                   <  1:9584378/93‑1 (MQ=255)
        gggcggAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 <  1:3816701/93‑1 (MQ=255)
        gggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 <  2:3485467/93‑1 (MQ=255)
        gggcggAAACACCACGGCAGGCAGCAAAGGCCAGAACGCCCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 <  1:3064001/93‑1 (MQ=255)
         ggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGATTGaa                                                                                >  1:3427979/1‑93 (MQ=255)
         ggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaa                                                                                <  2:9468815/93‑1 (MQ=255)
          gcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaa                                                                               >  1:1984099/1‑93 (MQ=255)
           cggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaa                                                                              <  1:10017960/93‑1 (MQ=255)
              caaCAGCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTGCCATTGAGCGTGACTGAAAAAGt                                                                           <  1:9906844/92‑1 (MQ=255)
              aaaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGt                                                                           >  2:3029769/1‑93 (MQ=255)
               aaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGta                                                                          >  1:9768786/1‑92 (MQ=255)
               aaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTc                                                                          <  2:4334893/93‑1 (MQ=255)
                 caccacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAc                                                                        <  1:9081725/93‑1 (MQ=255)
                   ccacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCa                                                                      >  2:492497/1‑93 (MQ=255)
                     ccGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                                    <  2:1251125/92‑1 (MQ=255)
                     acGTCAGGCAGCAAAGGCCAGAACGCTCCAAAAGATCCACGTATTGGCAGTAAAACTCCTGTTCCATTGAGCGTGACGGAAAAAGTCACCaaa                                                                    >  2:8973555/1‑93 (MQ=255)
                     acGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                                    >  1:3967733/1‑93 (MQ=255)
                      cGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAc                                                                   <  2:1721362/93‑1 (MQ=255)
                      cGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAc                                                                   <  1:8914928/93‑1 (MQ=255)
                        tCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAg                                                                 >  2:793204/1‑93 (MQ=255)
                        tCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAg                                                                 <  1:7330879/93‑1 (MQ=255)
                             cagcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaa                                                            >  2:4703784/1‑93 (MQ=255)
                              agcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaaa                                                           <  1:5915452/93‑1 (MQ=255)
                              agcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaaa                                                           <  1:2368392/93‑1 (MQ=255)
                              agcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaaa                                                           >  2:2678469/1‑93 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          <  2:2210609/93‑1 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          <  2:2319402/93‑1 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          >  1:4827045/1‑93 (MQ=255)
                                caAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAcc                                                         >  1:3386945/1‑93 (MQ=255)
                                caAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAcc                                                         <  1:5103288/93‑1 (MQ=255)
                                caAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAcc                                                         <  2:3592710/93‑1 (MQ=255)
                                caAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAcc                                                         >  2:9679050/1‑93 (MQ=255)
                                 aaaGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCg                                                        >  2:4200533/1‑93 (MQ=255)
                                  aaGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGa                                                       <  2:372625/93‑1 (MQ=255)
                                   agtccAAAACGCACCAAAAGATCCACGTATTGGCAGTAAACCCCCTGTTCCATTGAGCTTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                                                      <  2:3251930/90‑1 (MQ=255)
                                    ggCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAag                                                     <  1:2241011/93‑1 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    <  2:8378927/93‑1 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    <  2:1828552/93‑1 (MQ=255)
                                      ccAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagag                                                   <  2:7280351/93‑1 (MQ=255)
                                      ccAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagag                                                   <  2:7763261/93‑1 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  <  2:5689851/93‑1 (MQ=255)
                                        aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTg                                                 <  1:4863560/93‑1 (MQ=255)
                                        aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTg                                                 >  1:2990860/1‑93 (MQ=255)
                                         gAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTga                                                >  1:8733497/1‑93 (MQ=255)
                                          aaCGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgag                                               >  2:446839/1‑93 (MQ=255)
                                           aCGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgaga                                              <  2:9054984/93‑1 (MQ=255)
                                              cACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAc                                           >  2:9216885/1‑93 (MQ=255)
                                              cACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAc                                           <  1:9323595/93‑1 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          <  2:8198979/93‑1 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          <  2:9147145/93‑1 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          <  2:6307994/93‑1 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGGAAAAATGACCAAACAGCACACACCGAAGAGCGAGAAACCTa                                        >  2:1118474/1‑93 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        <  1:7493010/93‑1 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        <  1:6889776/93‑1 (MQ=255)
                                                  aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                                       <  2:8870178/93‑1 (MQ=255)
                                                   aaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATg                                      <  1:8026993/93‑1 (MQ=255)
                                                    aaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGAGAGAAACCTATGc                                     >  2:6894901/1‑93 (MQ=255)
                                                        tCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTc                                 <  1:8941860/93‑1 (MQ=255)
                                                           aCGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAcc                              <  2:9578172/93‑1 (MQ=255)
                                                            agTATTGGCAGTAAAACCCCTGTTCCATTGAGCGCGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCg                             <  2:7477392/92‑1 (MQ=255)
                                                             gTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGc                            <  2:3893032/93‑1 (MQ=255)
                                                              tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                           <  1:8263737/93‑1 (MQ=255)
                                                               aTTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAg                          >  1:1325371/1‑93 (MQ=255)
                                                               aTTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAg                          >  2:5888804/1‑93 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCCCCGCAgg                         >  2:8568196/1‑93 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         >  2:9769554/1‑93 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         >  2:1397512/1‑93 (MQ=255)
                                                                  ggCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCg                       <  1:5860691/93‑1 (MQ=255)
                                                                  ggCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCg                       >  1:5619478/1‑93 (MQ=255)
                                                                   gCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCgg                      <  1:5350567/93‑1 (MQ=255)
                                                                       tAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGtt                  >  1:6496923/1‑93 (MQ=255)
                                                                         aaaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTgg                >  1:3671953/1‑93 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  1:1873225/93‑1 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  1:45704/93‑1 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG‑AGAGTGAGAAACCTATGCTTTCACCGCAGGGGGGGTTGGAAt             >  2:2863118/1‑93 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          >  2:8159996/4‑93 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          <  1:9209146/90‑1 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          <  2:677617/90‑1 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          >  2:9321822/4‑93 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          >  2:1572841/4‑93 (MQ=255)
                                                                                tgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACt         >  1:5743011/3‑93 (MQ=255)
                                                                                tgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACt         <  2:5429238/91‑1 (MQ=255)
                                                                                tgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACt         <  1:7232647/91‑1 (MQ=255)
                                                                                  ttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTgg       <  1:701859/93‑1 (MQ=255)
                                                                                   tCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGa      >  1:3224460/1‑93 (MQ=255)
                                                                                     cATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaaa    <  2:5791565/93‑1 (MQ=255)
                                                                                      aTTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAc   <  1:280910/93‑1 (MQ=255)
                                                                                      aTTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAc   <  2:1984099/93‑1 (MQ=255)
                                                                                       ttGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACg  >  2:5220536/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 299 total aligned reads displayed.
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  >  NC_000913/4049056‑4049241
                                                                                             |                                                                                            
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              >  SRR4302540.3238035/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              >  SRR4302540.2942755/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              >  SRR4302540.4898862/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              >  SRR4302540.4586329/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              >  SRR4302540.1902843/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              >  SRR4302540.4376464/1‑94 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302540.6558194/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302540.1817915/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             >  SRR4302540.1535524/1‑94 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302540.1478167/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302540.7146638/94‑1 (MQ=60)
  CCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGC                                                                                            <  SRR4302540.2559325/94‑1 (MQ=60)
   CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCG                                                                                           <  SRR4302540.5109082/94‑1 (MQ=60)
   CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCG                                                                                           <  SRR4302540.7256537/94‑1 (MQ=60)
   CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCG                                                                                           >  SRR4302540.8499569/1‑94 (MQ=60)
    GCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGT                                                                                          <  SRR4302540.6229017/94‑1 (MQ=60)
    GCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGT                                                                                          <  SRR4302540.6383265/94‑1 (MQ=60)
    GCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGT                                                                                          <  SRR4302540.8400020/94‑1 (MQ=60)
    GCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGT                                                                                          <  SRR4302540.6383266/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                         <  SRR4302540.6188287/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                         <  SRR4302540.9573008/94‑1 (MQ=60)
      GCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGA                                                                                        >  SRR4302540.7457391/1‑94 (MQ=60)
      GCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGA                                                                                        >  SRR4302540.2309078/1‑94 (MQ=60)
      GCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGA                                                                                        >  SRR4302540.8673994/1‑94 (MQ=60)
       CAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAC                                                                                       <  SRR4302540.4113429/94‑1 (MQ=60)
       CAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAC                                                                                       <  SRR4302540.5790265/94‑1 (MQ=60)
       CAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTAGTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAC                                                                                       >  SRR4302540.9830187/1‑94 (MQ=60)
        AGCGGGCGGAAACACCACGTTAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      <  SRR4302540.7863866/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      >  SRR4302540.8941363/1‑94 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      <  SRR4302540.1772735/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      <  SRR4302540.6790063/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      <  SRR4302540.5051423/94‑1 (MQ=60)
         GCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTG                                                                                     <  SRR4302540.43378/94‑1 (MQ=60)
         GCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTG                                                                                     >  SRR4302540.9245967/1‑94 (MQ=60)
         GCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTG                                                                                     <  SRR4302540.9697533/94‑1 (MQ=60)
           GGGCGGAAGCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   <  SRR4302540.3858255/94‑1 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGATTGAA                                                                                   >  SRR4302540.3465233/1‑94 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   >  SRR4302540.2060162/1‑94 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   <  SRR4302540.1708939/94‑1 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   <  SRR4302540.3097615/94‑1 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   >  SRR4302540.3465232/1‑94 (MQ=60)
           GGGCGGAAACACCACGGCAGGCAGCAAAGGCCAGAACGCCCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   <  SRR4302540.3097618/94‑1 (MQ=60)
            GGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAA                                                                                  >  SRR4302540.2005610/1‑94 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGCAAAA                                                                                >  SRR4302540.9195001/1‑94 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                                >  SRR4302540.6681831/1‑94 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                                <  SRR4302540.10134561/94‑1 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACACCTGTTCCATTGAGCGTGACTGAAAAA                                                                                >  SRR4302540.9194713/1‑94 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                               <  SRR4302540.2922085/94‑1 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                               <  SRR4302540.2518938/94‑1 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACACCTGTTCCATTGAGCGTGACTGAAAAAG                                                                               >  SRR4302540.5397944/1‑94 (MQ=60)
                GAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGT                                                                              <  SRR4302540.3906837/94‑1 (MQ=60)
                 CAACAGCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTGCCATTGAGCGTGACTGAAAAAGTC                                                                             <  SRR4302540.10022570/94‑1 (MQ=60)
                 AAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTC                                                                             <  SRR4302540.4017423/94‑1 (MQ=60)
                 AAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTA                                                                             >  SRR4302540.9883333/1‑94 (MQ=60)
                   ACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAC                                                                           <  SRR4302540.6346142/94‑1 (MQ=60)
                   ACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAC                                                                           >  SRR4302540.6643021/1‑94 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                         <  SRR4302540.7360270/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                        <  SRR4302540.7221056/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                        <  SRR4302540.6495171/94‑1 (MQ=60)
                       CACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAA                                                                       >  SRR4302540.1289780/1‑94 (MQ=60)
                       CACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAA                                                                       >  SRR4302540.4010507/1‑94 (MQ=60)
                        ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                                      <  SRR4302540.3384764/94‑1 (MQ=60)
                        ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                                      >  SRR4302540.7368842/1‑94 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                     <  SRR4302540.9018172/94‑1 (MQ=60)
                          ctgcgggAGCAAAGTCCAGAACGCACCAAAAGATCCACGTATTGTCAGTAAAGCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                    <  SRR4302540.2792719/87‑1 (MQ=60)
                          aacaggcagcaaaagccagaacacaccaaccacaCCACGTATTGGAAATAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAAAG                                                                    <  SRR4302540.9056361/60‑1 (MQ=42)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                    >  SRR4302540.7854694/1‑94 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                    <  SRR4302540.8710659/94‑1 (MQ=60)
                           TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                                   <  SRR4302540.7412720/94‑1 (MQ=60)
                              GGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACA                                                                <  SRR4302540.2832022/94‑1 (MQ=60)
                              GGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACA                                                                <  SRR4302540.3183394/94‑1 (MQ=60)
                               GCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAA                                                               <  SRR4302540.6021362/94‑1 (MQ=60)
                                CAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAA                                                              >  SRR4302540.10035129/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                             >  SRR4302540.5960544/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                             >  SRR4302540.4878736/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                             <  SRR4302540.5980801/94‑1 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                             <  SRR4302540.2393893/94‑1 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                             >  SRR4302540.4195508/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                             <  SRR4302540.5980800/94‑1 (MQ=60)
                                  GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                            <  SRR4302540.9956867/94‑1 (MQ=60)
                                  GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                            <  SRR4302540.1617063/94‑1 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                           <  SRR4302540.5158527/94‑1 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                           >  SRR4302540.2777555/1‑94 (MQ=60)
                                    AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                          >  SRR4302540.4157898/1‑94 (MQ=60)
                                     AAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAA                                                         >  SRR4302540.2208050/1‑94 (MQ=60)
                                     AAGGCCAGAACCCCCCAAAAGACCCACGTATTGGCAGTAAACCCCCCGTTCCATTGAGCGTGACTGAAAAAGTCCCCAAACAGCACAAACCGAA                                                         <  SRR4302540.9119431/94‑1 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                                        >  SRR4302540.4034136/1‑94 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                                        <  SRR4302540.3429402/94‑1 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAATCAGCACAAACCGAAGA                                                       >  SRR4302540.9028316/1‑94 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                       <  SRR4302540.2265096/94‑1 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                       <  SRR4302540.7795243/94‑1 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                       <  SRR4302540.8098899/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                      <  SRR4302540.4799474/94‑1 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                                     <  SRR4302540.1967026/94‑1 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCATCAAACAGTACAAACCGAAGAGGG                                                    >  SRR4302540.9059984/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                    <  SRR4302540.9757569/94‑1 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                    >  SRR4302540.3838622/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                    >  SRR4302540.3023832/1‑94 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   >  SRR4302540.8834911/1‑94 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   <  SRR4302540.1952863/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   <  SRR4302540.4915721/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   >  SRR4302540.6476101/1‑94 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                  <  SRR4302540.946728/94‑1 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                  <  SRR4302540.5739393/94‑1 (MQ=60)
                                             AACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTTACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGA                                                 <  SRR4302540.6792012/94‑1 (MQ=60)
                                             AACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGA                                                 <  SRR4302540.5930057/94‑1 (MQ=60)
                                              ACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAA                                                <  SRR4302540.9341287/94‑1 (MQ=60)
                                                GCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                              >  SRR4302540.3289005/1‑94 (MQ=60)
                                                GCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                              <  SRR4302540.3641897/94‑1 (MQ=60)
                                                GCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                              <  SRR4302540.4677390/94‑1 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            <  SRR4302540.8832496/94‑1 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            >  SRR4302540.2320624/1‑94 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            >  SRR4302540.1875964/1‑94 (MQ=60)
                                                   CCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTA                                           <  SRR4302540.903159/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                          <  SRR4302540.7576685/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                          <  SRR4302540.5950828/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                          <  SRR4302540.949923/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                          <  SRR4302540.5427946/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                          <  SRR4302540.10162589/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                          <  SRR4302540.9153448/94‑1 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                         >  SRR4302540.4613947/1‑94 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                                        <  SRR4302540.8120337/94‑1 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                                        <  SRR4302540.7544726/94‑1 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAACCCGAAGAGTGAGAAACCTATGCT                                       <  SRR4302540.1838368/94‑1 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                      <  SRR4302540.3213938/94‑1 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                    <  SRR4302540.7458632/94‑1 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                    <  SRR4302540.875970/94‑1 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                    <  SRR4302540.9420836/94‑1 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                    <  SRR4302540.6991429/94‑1 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCC                                   >  SRR4302540.2205065/1‑94 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                   >  SRR4302540.551083/1‑94 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                   >  SRR4302540.3569415/1‑94 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                   >  SRR4302540.3007048/1‑94 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                  <  SRR4302540.8469713/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                  <  SRR4302540.3980856/94‑1 (MQ=60)
                                                             CACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACC                                 <  SRR4302540.9293864/94‑1 (MQ=60)
                                                             CACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTAAGAAACCTATGCTTTCACC                                 >  SRR4302540.2402838/1‑94 (MQ=60)
                                                              ACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCG                                <  SRR4302540.4548601/94‑1 (MQ=60)
                                                                GTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCA                              >  SRR4302540.1091162/1‑94 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                             >  SRR4302540.1339195/1‑94 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                             <  SRR4302540.8359762/94‑1 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                            <  SRR4302540.495694/94‑1 (MQ=60)
                                                                   TTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGC                           >  SRR4302540.5916915/1‑94 (MQ=60)
                                                                   TTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGC                           <  SRR4302540.5329785/94‑1 (MQ=60)
                                                                    TGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCG                          >  SRR4302540.2428643/1‑94 (MQ=60)
                                                                    TGGCAGTAAAACCCCTGTTCAATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCG                          >  SRR4302540.5896920/1‑94 (MQ=60)
                                                                     GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                         <  SRR4302540.8165509/94‑1 (MQ=60)
                                                                     GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                         <  SRR4302540.8165510/94‑1 (MQ=60)
                                                                     GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                         <  SRR4302540.5925500/94‑1 (MQ=60)
                                                                      GCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGA                        <  SRR4302540.6261884/94‑1 (MQ=60)
                                                                       CAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAG                       <  SRR4302540.9402407/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGCGT                      >  SRR4302540.1370052/1‑94 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      <  SRR4302540.6972564/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      <  SRR4302540.1186881/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      >  SRR4302540.2571384/1‑94 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      >  SRR4302540.1062538/1‑94 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      >  SRR4302540.5174765/1‑94 (MQ=60)
                                                                         GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT                     <  SRR4302540.901561/94‑1 (MQ=60)
                                                                         GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT                     >  SRR4302540.3998516/1‑94 (MQ=60)
                                                                         GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT                     >  SRR4302540.3070193/1‑94 (MQ=60)
                                                                          TAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTG                    >  SRR4302540.5681939/1‑94 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAACGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                   >  SRR4302540.3712111/1‑94 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                   >  SRR4302540.3712112/1‑94 (MQ=60)
                                                                            AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                  >  SRR4302540.4752414/1‑94 (MQ=60)
                                                                             AACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAT                 >  SRR4302540.3336459/1‑94 (MQ=60)
                                                                             AACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAA                 >  SRR4302540.5436421/1‑94 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                <  SRR4302540.46171/94‑1 (MQ=60)
                                                                               CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT               <  SRR4302540.8862000/94‑1 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA              <  SRR4302540.3360745/94‑1 (MQ=60)
                                                                                 CCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAC             <  SRR4302540.3081499/94‑1 (MQ=60)
                                                                                 CCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAC             <  SRR4302540.4885836/94‑1 (MQ=60)
                                                                                 CCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGGTGGAATTAC             >  SRR4302540.1199305/1‑94 (MQ=60)
                                                                                 CCTGTGCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAC             <  SRR4302540.1293726/94‑1 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT            <  SRR4302540.1901524/94‑1 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT            >  SRR4302540.7976917/1‑94 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT            >  SRR4302540.5806323/1‑94 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT            >  SRR4302540.5616353/1‑94 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT            <  SRR4302540.4894378/94‑1 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG           <  SRR4302540.3824918/94‑1 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG           >  SRR4302540.3451966/1‑94 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG           >  SRR4302540.7171587/1‑94 (MQ=60)
                                                                                   TGTTCCATTGAGCGTAACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG           <  SRR4302540.7313313/94‑1 (MQ=60)
                                                                                     TTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGA         >  SRR4302540.3259511/1‑94 (MQ=60)
                                                                                     TTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGA         <  SRR4302540.709393/94‑1 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA        <  SRR4302540.2343805/94‑1 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA        >  SRR4302540.5032906/1‑94 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA        <  SRR4302540.9323822/94‑1 (MQ=60)
                                                                                       CCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAA       >  SRR4302540.9965545/1‑94 (MQ=60)
                                                                                        CATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAcaa      >  SRR4302540.6295648/1‑91 (MQ=60)
                                                                                        CATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAC      >  SRR4302540.8034397/1‑94 (MQ=60)
                                                                                        CATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAC      >  SRR4302540.7056292/1‑94 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGGGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG     <  SRR4302540.10034937/94‑1 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG     >  SRR4302540.8898690/1‑94 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG     <  SRR4302540.9788475/94‑1 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG     <  SRR4302540.283666/94‑1 (MQ=60)
                                                                                          TTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGG    >  SRR4302540.397698/1‑94 (MQ=60)
                                                                                           TGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGA   <  SRR4302540.4516074/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  <  SRR4302540.7715602/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  <  SRR4302540.6504086/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  <  SRR4302540.4201156/94‑1 (MQ=60)
                                                                                             |                                                                                            
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  >  NC_000913/4049056‑4049241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑