Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,049,149 G→A G75S (GGC→AGC)  yihI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,049,1490GA99.6% 956.4 / ‑5.6 254G75S (GGC→AGC) yihIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (120/132);  total (122/132)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 257 total aligned reads displayed.

CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238
                                                                                          |                                                                                         
cgcgCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGc                                                                                         <  2:4052515/93‑1 (MQ=255)
cgcgCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGc                                                                                         <  1:5247099/93‑1 (MQ=255)
  cgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGt                                                                                       <  1:2603540/93‑1 (MQ=255)
   gcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTg                                                                                      >  2:3106506/1‑93 (MQ=255)
   gcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTg                                                                                      >  2:597362/1‑93 (MQ=255)
     aGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                                    <  2:1716882/93‑1 (MQ=255)
     aGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                                    <  1:5504257/93‑1 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                                   >  1:1111510/1‑93 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                                   >  2:3523259/1‑93 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                                   >  1:3488727/1‑93 (MQ=255)
        gggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 >  1:4840147/1‑93 (MQ=255)
        gggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 <  1:1916772/93‑1 (MQ=255)
           cggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaa                                                                              <  1:4095490/93‑1 (MQ=255)
            ggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaaa                                                                             <  1:3905369/93‑1 (MQ=255)
            ggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaaa                                                                             <  1:1349293/93‑1 (MQ=255)
             gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGCTCCACGTATTGGCAGTAAAACACCTGTTCCATTGAGCGTGACTGAAAAAg                                                                            >  2:1609992/1‑93 (MQ=255)
             gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAg                                                                            >  1:2020818/1‑93 (MQ=255)
             gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAg                                                                            <  1:5788809/93‑1 (MQ=255)
                acacCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCa                                                                         >  2:424369/1‑93 (MQ=255)
                acacCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCa                                                                         >  1:3951001/1‑93 (MQ=255)
                acacCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCa                                                                         >  1:310659/1‑93 (MQ=255)
                acacCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCa                                                                         <  1:6141702/93‑1 (MQ=255)
                  accacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAcc                                                                       <  1:2396376/93‑1 (MQ=255)
                   ccacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCa                                                                      >  1:6016942/1‑93 (MQ=255)
                     acGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                                    >  1:439834/1‑93 (MQ=255)
                     acGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                                    >  1:226472/1‑93 (MQ=255)
                      cGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAc                                                                   <  1:4651727/93‑1 (MQ=255)
                      cGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAc                                                                   <  1:6104493/93‑1 (MQ=255)
                       gTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                                  >  1:2233691/1‑93 (MQ=255)
                        tCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAg                                                                 <  2:773510/93‑1 (MQ=255)
                         cAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGc                                                                <  1:2311736/93‑1 (MQ=255)
                          aGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGca                                                               <  2:1010232/93‑1 (MQ=255)
                           ggCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGcac                                                              <  2:2331436/93‑1 (MQ=255)
                             cagcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaa                                                            <  2:4314947/93‑1 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          >  2:2299995/1‑93 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          >  1:2871996/1‑93 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          >  1:6194798/1‑93 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCCGTTCCATTGAGCGTGAATGAAAAAGTCACCAAACAGCACAAAc                                                          >  1:5927333/1‑93 (MQ=255)
                                 aaaGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCg                                                        >  2:5822223/1‑93 (MQ=255)
                                  aaGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGa                                                       >  1:3759337/1‑93 (MQ=255)
                                   aGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                                                      >  2:3789257/1‑93 (MQ=255)
                                   aGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                                                      <  2:1437579/93‑1 (MQ=255)
                                   aGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                                                      <  1:2650295/93‑1 (MQ=255)
                                    ggCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAag                                                     <  2:4872789/93‑1 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    <  2:4381403/93‑1 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    >  2:5360945/1‑93 (MQ=255)
                                      ccAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagag                                                   >  1:1736730/1‑93 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  >  2:694469/1‑93 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  >  1:3604846/1‑93 (MQ=255)
                                        aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTg                                                 >  1:5238383/1‑93 (MQ=255)
                                          aaCGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgag                                               <  2:4502859/93‑1 (MQ=255)
                                             gCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGaaa                                            <  2:332697/93‑1 (MQ=255)
                                             gCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGaaa                                            >  2:2419759/1‑93 (MQ=255)
                                              cACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAc                                           >  2:4819239/1‑93 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          <  2:410912/93‑1 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          <  2:499981/93‑1 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          >  2:5033788/1‑93 (MQ=255)
                                                ccAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCt                                         >  2:5742483/1‑93 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        <  1:4002103/93‑1 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        <  1:1791345/93‑1 (MQ=255)
                                                  aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                                       <  1:143988/93‑1 (MQ=255)
                                                   aaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATg                                      <  1:907610/93‑1 (MQ=255)
                                                    aaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGc                                     <  1:5664821/93‑1 (MQ=255)
                                                     aGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCt                                    <  1:1579946/93‑1 (MQ=255)
                                                       aTCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCttt                                  <  1:4234706/93‑1 (MQ=255)
                                                          cACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAc                               >  2:3032301/1‑93 (MQ=255)
                                                            cGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCg                             <  1:3788913/93‑1 (MQ=255)
                                                              tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                           <  2:618593/93‑1 (MQ=255)
                                                              tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                           >  2:2526028/1‑93 (MQ=255)
                                                               aTTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAg                          >  2:4128903/1‑93 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         <  1:6075281/93‑1 (MQ=255)
                                                                  ggCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCg                       <  2:4538551/93‑1 (MQ=255)
                                                                    cAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGa                     >  1:2152415/1‑93 (MQ=255)
                                                                    cAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGa                     >  2:3607415/1‑93 (MQ=255)
                                                                        aaaaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTg                 <  2:5291919/93‑1 (MQ=255)
                                                                         aaaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTgg                <  1:1513197/93‑1 (MQ=255)
                                                                         aaaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTgg                <  1:2532127/93‑1 (MQ=255)
                                                                          aaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGa               >  2:4611536/1‑93 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCTAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  1:3389559/93‑1 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  1:105696/93‑1 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  1:5619119/93‑1 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  1:2908340/93‑1 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  2:2806173/93‑1 (MQ=255)
                                                                              ccTGTTCCATTGCGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           >  2:1925764/1‑93 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           >  2:5511144/1‑93 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           >  2:4767634/1‑93 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           >  2:5192235/1‑93 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          <  1:5042462/90‑1 (MQ=255)
                                                                                tgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACt         <  2:2691503/91‑1 (MQ=255)
                                                                                 gttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTg        >  1:5302497/2‑93 (MQ=255)
                                                                                 gttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTg        >  2:5571527/2‑93 (MQ=255)
                                                                                  ttCCATCGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTgg       <  2:1249460/93‑1 (MQ=255)
                                                                                   tCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGa      <  2:4652939/93‑1 (MQ=255)
                                                                                     cATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaaa    >  2:271351/1‑93 (MQ=255)
                                                                                      aTTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAc   >  2:1059578/1‑93 (MQ=255)
                                                                                      aTTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAc   <  2:872190/93‑1 (MQ=255)
                                                                                       ttGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACg  >  2:5906794/1‑93 (MQ=255)
                                                                                       ttGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACg  >  1:1711648/1‑93 (MQ=255)
                                                                                       ttGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACg  >  1:4081895/1‑93 (MQ=255)
                                                                                       ttGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACg  <  2:3666987/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  >  NC_000913/4049056‑4049242
                                                                                             |                                                                                             
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                               <  SRR4302533.4419024/94‑1 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                               <  SRR4302533.1167727/94‑1 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                               >  SRR4302533.2313457/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                               >  SRR4302533.5493755/1‑94 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                              <  SRR4302533.5417995/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                              <  SRR4302533.1418228/94‑1 (MQ=60)
  CCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGC                                                                                             <  SRR4302533.5683646/94‑1 (MQ=60)
   CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCG                                                                                            <  SRR4302533.5278647/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                          <  SRR4302533.2619534/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                          <  SRR4302533.1835696/94‑1 (MQ=60)
      GCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGA                                                                                         <  SRR4302533.4326114/94‑1 (MQ=60)
       CAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAC                                                                                        >  SRR4302533.231343/1‑94 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                       <  SRR4302533.5537467/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                       >  SRR4302533.1118106/1‑94 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                       >  SRR4302533.3510242/1‑94 (MQ=60)
          CGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGGGCGTGACTGA                                                                                     >  SRR4302533.4704310/1‑94 (MQ=60)
          CGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGA                                                                                     >  SRR4302533.4869482/1‑94 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                    <  SRR4302533.1928473/94‑1 (MQ=60)
             GCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGTCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAA                                                                                  >  SRR4302533.6174967/1‑94 (MQ=60)
             GCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAA                                                                                  <  SRR4302533.3709551/94‑1 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                                 >  SRR4302533.423650/1‑94 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                                 <  SRR4302533.4120422/94‑1 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                                <  SRR4302533.3929220/94‑1 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                                <  SRR4302533.1357410/94‑1 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                                >  SRR4302533.2033263/1‑94 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                                >  SRR4302533.5722171/1‑94 (MQ=60)
                GAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGT                                                                               <  SRR4302533.5823786/94‑1 (MQ=60)
                 AAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTC                                                                              <  SRR4302533.3201568/94‑1 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                             >  SRR4302533.312561/1‑94 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                             >  SRR4302533.3975087/1‑94 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                             <  SRR4302533.2130842/94‑1 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                             >  SRR4302533.5182543/1‑94 (MQ=60)
                   ACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAC                                                                            <  SRR4302533.6178690/94‑1 (MQ=60)
                    CACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTCGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAGAGTCACC                                                                           >  SRR4302533.527580/1‑94 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                          <  SRR4302533.2411169/94‑1 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                          >  SRR4302533.6053399/1‑94 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                          >  SRR4302533.2270468/1‑94 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                          <  SRR4302533.186860/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                         <  SRR4302533.3943111/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                         <  SRR4302533.3465423/94‑1 (MQ=60)
                       CACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAA                                                                        >  SRR4302533.442458/1‑94 (MQ=60)
                       CACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAA                                                                        >  SRR4302533.227883/1‑94 (MQ=60)
                        ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                                       <  SRR4302533.2490349/94‑1 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      <  SRR4302533.6141339/94‑1 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      >  SRR4302533.2247474/1‑94 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      >  SRR4302533.2083177/1‑94 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      <  SRR4302533.2623881/94‑1 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      <  SRR4302533.4679914/94‑1 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                     <  SRR4302533.6261347/94‑1 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                     <  SRR4302533.4043184/94‑1 (MQ=60)
                           TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                                    >  SRR4302533.1367683/1‑94 (MQ=60)
                           TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                                    >  SRR4302533.5540133/1‑94 (MQ=60)
                            CAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCA                                                                   <  SRR4302533.2326057/94‑1 (MQ=60)
                            CAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCA                                                                   <  SRR4302533.2213301/94‑1 (MQ=60)
                                CAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAA                                                               <  SRR4302533.5285957/94‑1 (MQ=60)
                                CAGCAAAGCCCAGAACGCACCAAAAGACCCTCGTATTGACAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAA                                                               <  SRR4302533.5285958/94‑1 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              >  SRR4302533.6102772/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              >  SRR4302533.238805/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              <  SRR4302533.305580/94‑1 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              >  SRR4302533.2889667/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              >  SRR4302533.6231967/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCCGTTCCATTGAGCGTGAATGAAAAAGTCACCAAACAGCACAAAC                                                              >  SRR4302533.5963166/1‑94 (MQ=60)
                                  GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                             >  SRR4302533.5744455/1‑94 (MQ=60)
                                  GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                             <  SRR4302533.940161/94‑1 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                            <  SRR4302533.5267909/94‑1 (MQ=60)
                                    AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                           >  SRR4302533.2663272/1‑94 (MQ=60)
                                    AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                           >  SRR4302533.3782382/1‑94 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                                         <  SRR4302533.2666575/94‑1 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                                         <  SRR4302533.728715/94‑1 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                        <  SRR4302533.5413445/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                       >  SRR4302533.2700290/1‑94 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                       >  SRR4302533.1747269/1‑94 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                                      >  SRR4302533.3627034/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                     >  SRR4302533.5269879/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                     <  SRR4302533.3783856/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                    <  SRR4302533.1606870/94‑1 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                   >  SRR4302533.4117231/1‑94 (MQ=60)
                                             AACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGA                                                  >  SRR4302533.4013798/1‑94 (MQ=60)
                                              ACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAA                                                 >  SRR4302533.2906201/1‑94 (MQ=60)
                                                GCACCAAAAGATCCACGTATTGTCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                               <  SRR4302533.1778601/94‑1 (MQ=60)
                                                GCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                               >  SRR4302533.2572281/1‑94 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                              >  SRR4302533.5983012/1‑94 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                             >  SRR4302533.4016312/1‑94 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                           <  SRR4302533.5878189/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                           <  SRR4302533.4026474/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                           <  SRR4302533.1802237/94‑1 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                          <  SRR4302533.2018388/94‑1 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                          <  SRR4302533.144911/94‑1 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                                         <  SRR4302533.1685607/94‑1 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                                         <  SRR4302533.912932/94‑1 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                                        <  SRR4302533.3836974/94‑1 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                                        <  SRR4302533.5699047/94‑1 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                       >  SRR4302533.3763687/1‑94 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                       <  SRR4302533.1589464/94‑1 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                       >  SRR4302533.6057343/1‑94 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                     >  SRR4302533.1733982/1‑94 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                     >  SRR4302533.1567851/1‑94 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                     <  SRR4302533.4260294/94‑1 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                    >  SRR4302533.5874329/1‑94 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   >  SRR4302533.3272894/1‑94 (MQ=60)
                                                             CACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACC                                  >  SRR4302533.6133861/1‑94 (MQ=60)
                                                              ACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCG                                 >  SRR4302533.2455748/1‑94 (MQ=60)
                                                               CGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGC                                >  SRR4302533.1283794/1‑94 (MQ=60)
                                                               CGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGC                                <  SRR4302533.3812115/94‑1 (MQ=60)
                                                                GTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCA                               >  SRR4302533.1459381/1‑94 (MQ=60)
                                                                   TTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGC                            <  SRR4302533.6112029/94‑1 (MQ=60)
                                                                     GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                          <  SRR4302533.2911222/94‑1 (MQ=60)
                                                                      GCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGA                         >  SRR4302533.2165674/1‑94 (MQ=60)
                                                                        AGTAAAGCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                       <  SRR4302533.4057796/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                       >  SRR4302533.1748181/1‑94 (MQ=60)
                                                                         GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT                      <  SRR4302533.235584/94‑1 (MQ=60)
                                                                         GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT                      >  SRR4302533.5669955/1‑94 (MQ=60)
                                                                          TAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTG                     >  SRR4302533.1449139/1‑94 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                    <  SRR4302533.3839386/94‑1 (MQ=60)
                                                                            AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                   <  SRR4302533.1522313/94‑1 (MQ=60)
                                                                            AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                   <  SRR4302533.2547715/94‑1 (MQ=60)
                                                                            AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                   >  SRR4302533.4562907/1‑94 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCTAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 <  SRR4302533.3410491/94‑1 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 <  SRR4302533.5653081/94‑1 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 <  SRR4302533.2926194/94‑1 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 <  SRR4302533.6204116/94‑1 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 <  SRR4302533.106355/94‑1 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTCTCACCGCAGGCGGAGTTGGAAT                 >  SRR4302533.965087/1‑94 (MQ=60)
                                                                              ACCCCTCTTCCATTGAGCGTGACTGAAAAAGCCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 <  SRR4302533.1110365/94‑1 (MQ=60)
                                                                               CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT                >  SRR4302533.1257529/1‑94 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT             <  SRR4302533.2252733/94‑1 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT             <  SRR4302533.5072980/94‑1 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT             <  SRR4302533.4896381/94‑1 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG            >  SRR4302533.5334265/1‑94 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG            >  SRR4302533.4530823/1‑94 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG            <  SRR4302533.5580500/94‑1 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA         <  SRR4302533.5055617/94‑1 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA         <  SRR4302533.3603859/94‑1 (MQ=60)
                                                                                        CATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAC       <  SRR4302533.6178685/94‑1 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG      >  SRR4302533.4106744/1‑94 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG      >  SRR4302533.341189/1‑94 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG      >  SRR4302533.1722022/1‑94 (MQ=60)
                                                                                          TTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGG     <  SRR4302533.4207257/94‑1 (MQ=60)
                                                                                           TGAGCGTGACTGAAAAAGTCACCAAGCAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAACCGGA    <  SRR4302533.6033155/94‑1 (MQ=60)
                                                                                           TGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGA    >  SRR4302533.3489507/1‑94 (MQ=60)
                                                                                           TGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGA    >  SRR4302533.126381/1‑94 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT   >  SRR4302533.6031998/1‑94 (MQ=60)
                                                                                             AGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  >  SRR4302533.5035490/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  >  NC_000913/4049056‑4049242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑