Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,052,844 T→C I5M (ATA→ATG)  glnG ← GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,052,8440TC99.6% 779.3 / ‑4.8 244I5M (ATA→ATG) glnGGlnG
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (114/128);  total (115/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

Only 100 of 244 total aligned reads displayed.

GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT  >  NC_000913/4052754‑4052933
                                                                                          |                                                                                         
gctctcAAACGTCGTACAGGTTAAACCTCCCCTAGCGAGCGCACGTTCAAGCTCCCAACGGATGGAACTATCGTCATCGACTTCCCAGACCAt                                                                                         <  2:1970909/91‑1 (MQ=255)
gTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAt                                                                                         >  1:1667183/1‑93 (MQ=255)
 ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATc                                                                                        >  2:3814863/1‑93 (MQ=255)
 ttCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATc                                                                                        >  2:3199776/1‑93 (MQ=255)
 ttCTCAAACGTCGTACAGGATAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATc                                                                                        >  2:6128914/1‑93 (MQ=255)
   ctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATccc                                                                                      >  1:3666940/1‑93 (MQ=255)
   ctcAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATccc                                                                                      >  1:1919639/1‑93 (MQ=255)
     cAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTc                                                                                    >  2:3518322/1‑93 (MQ=255)
     cAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTc                                                                                    >  2:5531975/1‑93 (MQ=255)
     cAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTc                                                                                    <  2:2978228/93‑1 (MQ=255)
     cAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTc                                                                                    <  1:2035475/93‑1 (MQ=255)
      aaaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCg                                                                                   <  2:2665809/93‑1 (MQ=255)
      aaaCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCg                                                                                   >  2:1056830/1‑93 (MQ=255)
        aCGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGtt                                                                                 >  2:2759312/1‑93 (MQ=255)
         cgtcgtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTg                                                                                <  2:1653769/93‑1 (MQ=255)
             gtACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATa                                                                            <  1:2235943/93‑1 (MQ=255)
              tACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCAtga                                                                           >  2:2263319/1‑91 (MQ=255)
               aCAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATaaa                                                                          <  2:639443/93‑1 (MQ=255)
                cAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAc                                                                         <  2:24137/93‑1 (MQ=255)
                cAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAc                                                                         >  2:4116780/1‑93 (MQ=255)
                 aGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACg                                                                        <  1:3258645/93‑1 (MQ=255)
                  ggTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACACAACGGATGGAACTAACGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGt                                                                       <  1:6298552/93‑1 (MQ=255)
                   gTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTc                                                                      <  2:2556467/93‑1 (MQ=255)
                    ttAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCa                                                                     <  2:3213573/93‑1 (MQ=255)
                      aaaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCAcc                                                                   <  1:46267/93‑1 (MQ=255)
                       aaCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATACACGTCACCt                                                                  >  1:3282748/1‑93 (MQ=255)
                        aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCtt                                                                 <  1:6143512/93‑1 (MQ=255)
                        aCCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCtt                                                                 >  1:5302563/1‑93 (MQ=255)
                          cTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTa                                                               <  1:2657007/93‑1 (MQ=255)
                          cTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTa                                                               >  2:5132873/1‑93 (MQ=255)
                            gCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAtt                                                             <  2:2205095/93‑1 (MQ=255)
                            gCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAtt                                                             >  1:247753/1‑93 (MQ=255)
                              cccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTc                                                           >  1:3960216/1‑93 (MQ=255)
                              cccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTc                                                           >  2:695682/1‑93 (MQ=255)
                               ccAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTct                                                          >  2:604796/1‑92 (MQ=255)
                                cAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCt                                                         >  1:2403874/1‑93 (MQ=255)
                                 agcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTg                                                        >  2:1853737/1‑93 (MQ=255)
                                 agcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTg                                                        <  1:3905616/93‑1 (MQ=255)
                                  gcgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGa                                                       >  2:5628761/1‑93 (MQ=255)
                                   cgagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGAt                                                      <  1:3083532/93‑1 (MQ=255)
                                    gagcgCACGTTCAAGCACCCAACGGATGGAAGTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATa                                                     <  2:1266406/93‑1 (MQ=255)
                                    gagcgCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATa                                                     <  2:2445168/93‑1 (MQ=255)
                                      gcgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAgg                                                   <  2:1452260/93‑1 (MQ=255)
                                      gcgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAgg                                                   >  1:4052938/1‑93 (MQ=255)
                                       cgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGc                                                  <  1:3745776/93‑1 (MQ=255)
                                       cgcACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGc                                                  >  2:4541159/1‑93 (MQ=255)
                                          aCGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAgg                                               >  2:3638707/1‑93 (MQ=255)
                                           cGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGt                                              >  1:148580/1‑93 (MQ=255)
                                            gTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTa                                             >  2:4822406/1‑93 (MQ=255)
                                             ttCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTaa                                            >  2:1006820/1‑93 (MQ=255)
                                               cAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                          <  2:3894048/93‑1 (MQ=255)
                                               cAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAc                                          <  2:622066/93‑1 (MQ=255)
                                                aaGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAcc                                         <  1:4760622/93‑1 (MQ=255)
                                                   cACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCgag                                      <  1:2608945/93‑1 (MQ=255)
                                                    aCCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCCGATAGGCAGGTAAACCgaga                                     >  2:4195051/1‑93 (MQ=255)
                                                     cccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGaa                                    <  1:717381/93‑1 (MQ=255)
                                                     cccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGaa                                    >  2:5040672/1‑93 (MQ=255)
                                                      ccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAc                                   >  1:1071633/1‑93 (MQ=255)
                                                      ccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAc                                   <  1:6074516/93‑1 (MQ=255)
                                                      ccAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAc                                   >  1:5939571/1‑93 (MQ=255)
                                                       cAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACt                                  <  1:4588029/93‑1 (MQ=255)
                                                        aaCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTc                                 <  2:2925335/93‑1 (MQ=255)
                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                <  2:162866/93‑1 (MQ=255)
                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                <  2:4911528/93‑1 (MQ=255)
                                                         aCGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCg                                >  1:5406545/1‑93 (MQ=255)
                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             >  2:19111/1‑93 (MQ=255)
                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             >  1:4277660/1‑93 (MQ=255)
                                                            gATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTa                             <  1:421245/93‑1 (MQ=255)
                                                             aTGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTAt                            <  1:135036/93‑1 (MQ=255)
                                                               ggAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGc                          >  1:5187732/1‑93 (MQ=255)
                                                               ggAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGc                          >  1:2158871/1‑93 (MQ=255)
                                                                gAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGcc                         <  1:1225579/93‑1 (MQ=255)
                                                                gAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGcc                         <  2:1727277/93‑1 (MQ=255)
                                                                 aaCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGccc                        <  2:3302443/93‑1 (MQ=255)
                                                                  aCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCt                       <  1:2676710/93‑1 (MQ=255)
                                                                  aCTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCt                       <  1:316463/93‑1 (MQ=255)
                                                                      tCGTCATCGACTACCCAGATCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGcc                   >  2:6107004/1‑93 (MQ=255)
                                                                        gTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCaa                 >  1:6019603/1‑93 (MQ=255)
                                                                        gTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCaa                 <  2:5206783/93‑1 (MQ=255)
                                                                          cATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACt               <  2:1338322/93‑1 (MQ=255)
                                                                           aTCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTg              >  2:172254/1‑93 (MQ=255)
                                                                           aTCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTg              >  1:4898363/1‑93 (MQ=255)
                                                                            tCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTgg             <  2:761959/93‑1 (MQ=255)
                                                                            tCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTgg             <  1:2869669/93‑1 (MQ=255)
                                                                              gACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATTCCCTGGCCAACTggca           >  2:5283022/1‑91 (MQ=255)
                                                                              gACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTa           <  1:766337/93‑1 (MQ=255)
                                                                               aCTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaa          >  2:6230149/1‑93 (MQ=255)
                                                                               aCTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTaa          >  1:1446704/1‑93 (MQ=255)
                                                                                 tACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt        >  2:5014433/1‑93 (MQ=255)
                                                                                 tACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt        >  1:6128303/1‑93 (MQ=255)
                                                                                 tACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAt        >  2:407222/1‑93 (MQ=255)
                                                                                  aCCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAtt       >  1:5618815/1‑93 (MQ=255)
                                                                                    ccAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCa     >  1:585462/1‑93 (MQ=255)
                                                                                    ccAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCa     >  2:879267/1‑93 (MQ=255)
                                                                                     cAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCaa    <  1:4116780/93‑1 (MQ=255)
                                                                                     cAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCaa    >  2:5867157/1‑93 (MQ=255)
                                                                                      aGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAt   >  1:4452941/1‑93 (MQ=255)
                                                                                      aGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAt   >  1:6201103/1‑93 (MQ=255)
                                                                                       gACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt  <  2:5801740/93‑1 (MQ=255)
                                                                                       gACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAtt  >  1:1600282/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT  >  NC_000913/4052754‑4052933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGC  >  NC_000913/4052751‑4052937
                                                                                             |                                                                                             
GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACC                                                                                               <  SRR4302533.4488318/94‑1 (MQ=60)
GCCGTTCTCAAACGTCGTACAGGTTAAACCCGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACC                                                                                               >  SRR4302533.621493/1‑94 (MQ=60)
 CCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCA                                                                                              >  SRR4302533.5414034/1‑94 (MQ=60)
  CGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCAT                                                                                             >  SRR4302533.1677254/1‑94 (MQ=60)
   GTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATC                                                                                            >  SRR4302533.2082838/1‑94 (MQ=60)
    TTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCC                                                                                           <  SRR4302533.3944492/94‑1 (MQ=60)
    TTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCC                                                                                           <  SRR4302533.6228992/94‑1 (MQ=60)
     TCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCC                                                                                          >  SRR4302533.1931357/1‑94 (MQ=60)
     TCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCC                                                                                          >  SRR4302533.3689480/1‑94 (MQ=60)
      CTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCT                                                                                         <  SRR4302533.3285886/94‑1 (MQ=60)
        CAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCG                                                                                       <  SRR4302533.1592009/94‑1 (MQ=60)
        CAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCG                                                                                       <  SRR4302533.2303178/94‑1 (MQ=60)
        CAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCG                                                                                       <  SRR4302533.2048028/94‑1 (MQ=60)
         AAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGT                                                                                      >  SRR4302533.1375300/1‑94 (MQ=60)
          AACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTT                                                                                     <  SRR4302533.1510035/94‑1 (MQ=60)
          AACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTT                                                                                     <  SRR4302533.426691/94‑1 (MQ=60)
           ACGTCGTACTGTTTAAACCTACCCCAGCGAGCGCACGTTCAAGCACCCAATGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTG                                                                                    <  SRR4302533.3410029/94‑1 (MQ=60)
                GTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAA                                                                               <  SRR4302533.2249766/94‑1 (MQ=60)
                  ACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAC                                                                             >  SRR4302533.3114411/1‑94 (MQ=60)
                  ACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAC                                                                             <  SRR4302533.421860/94‑1 (MQ=60)
                  ACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAAC                                                                             <  SRR4302533.2043630/94‑1 (MQ=60)
                   CAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACG                                                                            <  SRR4302533.4362065/94‑1 (MQ=60)
                    AGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGT                                                                           <  SRR4302533.2208583/94‑1 (MQ=60)
                    AGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGT                                                                           <  SRR4302533.3278744/94‑1 (MQ=60)
                     GGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACACAACGGATGGAACTAACGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTC                                                                          <  SRR4302533.6336148/94‑1 (MQ=60)
                      GTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCA                                                                         <  SRR4302533.6293787/94‑1 (MQ=60)
                      GTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCA                                                                         <  SRR4302533.3043251/94‑1 (MQ=60)
                        TAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCNTCGACTACCCAGACCANCCCTCGTTGCATANANGNCNCC                                                                       >  SRR4302533.1798598/1‑94 (MQ=60)
                         AAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATACACGTCACCT                                                                      >  SRR4302533.3303013/1‑94 (MQ=60)
                         AAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCT                                                                      >  SRR4302533.3535075/1‑94 (MQ=60)
                         AAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCT                                                                      <  SRR4302533.46587/94‑1 (MQ=60)
                          AACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTT                                                                     >  SRR4302533.5334331/1‑94 (MQ=60)
                           ACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTT                                                                    <  SRR4302533.968241/94‑1 (MQ=60)
                           ACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTT                                                                    <  SRR4302533.6180505/94‑1 (MQ=60)
                             CTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAT                                                                  >  SRR4302533.2864599/1‑94 (MQ=60)
                             CTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTAT                                                                  <  SRR4302533.2673330/94‑1 (MQ=60)
                              TGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATT                                                                 >  SRR4302533.249282/1‑94 (MQ=60)
                                CCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTC                                                               >  SRR4302533.3984346/1‑94 (MQ=60)
                                  CCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCT                                                             >  SRR4302533.2418720/1‑94 (MQ=60)
                                   CAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTG                                                            <  SRR4302533.3722095/94‑1 (MQ=60)
                                    AGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGA                                                           <  SRR4302533.3929471/94‑1 (MQ=60)
                                      cgcggcCACGTCCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATA                                                         <  SRR4302533.1251711/88‑1 (MQ=60)
                                      CGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATA                                                         <  SRR4302533.717086/94‑1 (MQ=60)
                                      CGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATA                                                         <  SRR4302533.3102487/94‑1 (MQ=60)
                                       GAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAG                                                        >  SRR4302533.272861/1‑94 (MQ=60)
                                       GAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAG                                                        <  SRR4302533.5437875/94‑1 (MQ=60)
                                       GAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAG                                                        >  SRR4302533.2438600/1‑94 (MQ=60)
                                        AGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGG                                                       >  SRR4302533.4077619/1‑94 (MQ=60)
                                          GGCACGTTCAAGCACCCAACGGATGCAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                     <  SRR4302533.1158182/94‑1 (MQ=60)
                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                     >  SRR4302533.4679454/1‑94 (MQ=60)
                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                     >  SRR4302533.6030489/1‑94 (MQ=60)
                                          CGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCA                                                     <  SRR4302533.3768744/94‑1 (MQ=60)
                                           GCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAG                                                    <  SRR4302533.1446620/94‑1 (MQ=60)
                                            CACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGG                                                   >  SRR4302533.4574906/1‑94 (MQ=60)
                                             ACGTTCAAGCGCCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGT                                                  <  SRR4302533.5582724/94‑1 (MQ=60)
                                             ACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGT                                                  <  SRR4302533.4820726/94‑1 (MQ=60)
                                             ACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGT                                                  >  SRR4302533.149529/1‑94 (MQ=60)
                                                 TCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAC                                              >  SRR4302533.4298690/1‑94 (MQ=60)
                                                 TCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAAC                                              <  SRR4302533.6073155/94‑1 (MQ=60)
                                                  CAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACC                                             <  SRR4302533.6235698/94‑1 (MQ=60)
                                                  CAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACC                                             >  SRR4302533.1350527/1‑94 (MQ=60)
                                                   AAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCG                                            <  SRR4302533.4789504/94‑1 (MQ=60)
                                                    AGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGA                                           >  SRR4302533.6074519/1‑94 (MQ=60)
                                                      CACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGA                                         <  SRR4302533.2624964/94‑1 (MQ=60)
                                                      CACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGA                                         <  SRR4302533.1620574/94‑1 (MQ=60)
                                                       ACCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAA                                        <  SRR4302533.1793797/94‑1 (MQ=60)
                                                        CCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAC                                       >  SRR4302533.2435622/1‑94 (MQ=60)
                                                        CCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAC                                       >  SRR4302533.5975484/1‑94 (MQ=60)
                                                        CCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAC                                       >  SRR4302533.1077948/1‑94 (MQ=60)
                                                        CCCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAAC                                       <  SRR4302533.721612/94‑1 (MQ=60)
                                                         CCAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACT                                      <  SRR4302533.6111260/94‑1 (MQ=60)
                                                          CAACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTC                                     <  SRR4302533.4615819/94‑1 (MQ=60)
                                                           AACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCG                                    >  SRR4302533.5439033/1‑94 (MQ=60)
                                                            ACGGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGG                                   >  SRR4302533.4135465/1‑94 (MQ=60)
                                                              GGATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTA                                 >  SRR4302533.4303384/1‑94 (MQ=60)
                                                               GATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTAT                                <  SRR4302533.423765/94‑1 (MQ=60)
                                                               GATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTAT                                >  SRR4302533.5287948/1‑94 (MQ=60)
                                                                ATGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATG                               <  SRR4302533.135893/94‑1 (MQ=60)
                                                                 TGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGC                              >  SRR4302533.5219042/1‑94 (MQ=60)
                                                                 TGGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGC                              >  SRR4302533.2172164/1‑94 (MQ=60)
                                                                  GGAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCC                             <  SRR4302533.217305/94‑1 (MQ=60)
                                                                   GAACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCC                            <  SRR4302533.1232923/94‑1 (MQ=60)
                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                           <  SRR4302533.4636162/94‑1 (MQ=60)
                                                                    AACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCT                           >  SRR4302533.2679937/1‑94 (MQ=60)
                                                                     ACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTG                          <  SRR4302533.318401/94‑1 (MQ=60)
                                                                     ACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTG                          <  SRR4302533.3856893/94‑1 (MQ=60)
                                                                     ACTATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTG                          <  SRR4302533.2693122/94‑1 (MQ=60)
                                                                       TATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGC                        >  SRR4302533.5234744/1‑94 (MQ=60)
                                                                        ATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCC                       >  SRR4302533.1466955/1‑94 (MQ=60)
                                                                        ATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCC                       <  SRR4302533.4028694/94‑1 (MQ=60)
                                                                        ATCGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCC                       >  SRR4302533.3206150/1‑94 (MQ=60)
                                                                          CGTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAA                     >  SRR4302533.6056080/1‑94 (MQ=60)
                                                                           GTCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAAC                    <  SRR4302533.5565375/94‑1 (MQ=60)
                                                                            TCATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACT                   <  SRR4302533.3516582/94‑1 (MQ=60)
                                                                             CATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTG                  >  SRR4302533.4928044/1‑94 (MQ=60)
                                                                             CATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTG                  <  SRR4302533.5140245/94‑1 (MQ=60)
                                                                             CATCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTG                  <  SRR4302533.3917048/94‑1 (MQ=60)
                                                                               TCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGT                <  SRR4302533.827358/94‑1 (MQ=60)
                                                                               TCGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGT                <  SRR4302533.2887327/94‑1 (MQ=60)
                                                                                CGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTA               <  SRR4302533.6131962/94‑1 (MQ=60)
                                                                                CGACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTA               >  SRR4302533.1843262/1‑94 (MQ=60)
                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA              >  SRR4302533.2641162/1‑94 (MQ=60)
                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA              >  SRR4302533.769277/1‑94 (MQ=60)
                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA              <  SRR4302533.770858/94‑1 (MQ=60)
                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA              >  SRR4302533.1455434/1‑94 (MQ=60)
                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA              <  SRR4302533.6165848/94‑1 (MQ=60)
                                                                                 GACTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAA              <  SRR4302533.4727240/94‑1 (MQ=60)
                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT            <  SRR4302533.634797/94‑1 (MQ=60)
                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT            >  SRR4302533.2584755/1‑94 (MQ=60)
                                                                                   CTACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAAT            >  SRR4302533.6165236/1‑94 (MQ=60)
                                                                                    TACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATT           >  SRR4302533.5921936/1‑94 (MQ=60)
                                                                                    TACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATT           >  SRR4302533.2665924/1‑94 (MQ=60)
                                                                                    TACCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATT           >  SRR4302533.5652776/1‑94 (MQ=60)
                                                                                      CCCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCA         >  SRR4302533.588913/1‑94 (MQ=60)
                                                                                       CCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAA        >  SRR4302533.5928645/1‑94 (MQ=60)
                                                                                       CCAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAA        <  SRR4302533.3248886/94‑1 (MQ=60)
                                                                                        CAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAT       <  SRR4302533.4141787/94‑1 (MQ=60)
                                                                                        CAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAT       <  SRR4302533.4038062/94‑1 (MQ=60)
                                                                                        CAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAT       >  SRR4302533.4479863/1‑94 (MQ=60)
                                                                                        CAGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAAT       >  SRR4302533.6238296/1‑94 (MQ=60)
                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT      >  SRR4302533.5693515/1‑94 (MQ=60)
                                                                                         AGACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATT      >  SRR4302533.1609969/1‑94 (MQ=60)
                                                                                          GACCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTT     <  SRR4302533.4591658/94‑1 (MQ=60)
                                                                                            CCATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTG   >  SRR4302533.2466732/1‑94 (MQ=60)
                                                                                             CATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCCATTTTGC  >  SRR4302533.2189590/1‑94 (MQ=60)
                                                                                             CATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGC  <  SRR4302533.6322130/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCACCCAACGGATGGAACTATCGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGC  >  NC_000913/4052751‑4052937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑