Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,519 +T intergenic (‑268/‑530) cpxR ← / → yiiP transcriptional regulator in 2‑component system/putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5191.T99.5% 786.1 / ‑3.8 211intergenic (‑268/‑530)cpxR/yiiPtranscriptional regulator in 2‑component system/putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (93/117);  total (93/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 261 total aligned reads displayed.

GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605
                                                                                             |                                                                                      
ggCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcag                                                                                         <  1:5985682/93‑1 (MQ=255)
ggCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcag                                                                                         >  1:3740499/1‑93 (MQ=255)
ggCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcag                                                                                         <  2:1121340/93‑1 (MQ=255)
 gCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagt                                                                                        <  1:124370/93‑2 (MQ=255)
 gCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagt                                                                                        <  2:2989983/93‑2 (MQ=255)
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagta                                                                                       <  1:1534167/93‑3 (MQ=255)
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagta                                                                                       >  1:4414599/1‑91 (MQ=255)
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagta                                                                                       >  1:3716484/1‑91 (MQ=255)
   cTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtac                                                                                      >  2:5465993/1‑90 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  1:1608115/1‑89 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  1:5689845/1‑89 (MQ=255)
      aaCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgca                                                                                   >  2:437028/1‑93 (MQ=255)
      aaCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgca                                                                                   >  2:4988828/1‑93 (MQ=255)
       aCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcag                                                                                  <  2:4076298/93‑1 (MQ=255)
          cTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGc                                                                               <  2:3714331/93‑1 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              >  1:4045615/1‑93 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              >  1:2786487/1‑93 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCata                                                                           <  1:5228759/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCata                                                                           <  2:2410636/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCata                                                                           <  1:6203400/93‑1 (MQ=255)
                gtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATg                                                                         <  1:853573/93‑1 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGt                                                                        <  2:1557199/93‑1 (MQ=255)
                  cagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGtt                                                                       <  2:4273596/93‑1 (MQ=255)
                  cagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGtt                                                                       <  1:3000346/93‑1 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      >  1:3479084/1‑93 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      <  2:491056/93‑1 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      >  1:6036843/1‑93 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGa                                                                    <  1:3177448/93‑1 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGa                                                                    >  1:2497822/1‑93 (MQ=255)
                      tCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAc                                                                   >  2:1828453/1‑93 (MQ=255)
                      tCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAc                                                                   <  2:302004/93‑1 (MQ=255)
                          tAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCa                                                               <  1:2141818/93‑1 (MQ=255)
                            aGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATa                                                             <  2:1129324/93‑1 (MQ=255)
                            aGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATa                                                             <  2:2177307/93‑1 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATaa                                                            <  1:5088720/93‑1 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATaa                                                            <  1:4923872/93‑1 (MQ=255)
                               cACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGt                                                          >  1:3415399/1‑93 (MQ=255)
                                aCGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGtt                                                         >  2:241619/1‑93 (MQ=255)
                                aCGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGtt                                                         >  1:4733216/1‑93 (MQ=255)
                                aCGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGtt                                                         >  2:4774849/1‑93 (MQ=255)
                                  gctgctGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTa                                                       >  1:5277780/1‑93 (MQ=255)
                                  gctgctGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTa                                                       >  2:4535596/1‑93 (MQ=255)
                                   ctgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTaa                                                      <  1:2637132/93‑1 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     <  1:2280707/93‑1 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     <  2:5882835/93‑1 (MQ=255)
                                     gctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAcc                                                    <  1:4802880/93‑1 (MQ=255)
                                      ctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCg                                                   >  2:3964263/1‑93 (MQ=255)
                                        gAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGaa                                                 >  2:306819/1‑93 (MQ=255)
                                          aGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCTAACa                                               <  2:1791396/93‑1 (MQ=255)
                                          aGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACa                                               <  1:3155982/93‑1 (MQ=255)
                                          aGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACa                                               <  2:4057716/93‑1 (MQ=255)
                                             cGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCa                                            <  1:4581734/93‑1 (MQ=255)
                                              ggTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAg                                           <  1:5878525/93‑1 (MQ=255)
                                              ggTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAg                                           <  2:2348652/93‑1 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGc                                          >  2:699650/1‑93 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGc                                          >  2:2790328/1‑93 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGc                                          <  2:4470082/93‑1 (MQ=255)
                                                  cAGGCGATAACTGGCATCCGCGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTc                                       <  1:6128921/93‑1 (MQ=255)
                                                       gATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagcag                                  <  2:2061090/93‑1 (MQ=255)
                                                         tAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAt                                <  2:6045054/93‑1 (MQ=255)
                                                         tAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAt                                <  1:1065110/93‑1 (MQ=255)
                                                          aaCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATg                               >  2:6272915/1‑93 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              >  1:1859925/1‑93 (MQ=255)
                                                            cTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCg                             >  1:1988653/1‑93 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            <  2:4560049/93‑1 (MQ=255)
                                                              ggCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgag                           <  2:135739/93‑1 (MQ=255)
                                                              ggCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgag                           >  1:24051/1‑93 (MQ=255)
                                                              ggCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgag                           <  1:164592/93‑1 (MQ=255)
                                                               gCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgaga                          <  1:4543763/93‑1 (MQ=255)
                                                                cATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAt                         <  2:3583041/93‑1 (MQ=255)
                                                                cATCCGGGGGAAGAACTTACGCAGCGCAGTACGCATAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAt                         <  2:88405/93‑1 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        >  1:1915245/1‑93 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        <  1:64277/93‑1 (MQ=255)
                                                                  tCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCt                       >  2:5505677/1‑93 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     <  2:5488799/93‑1 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     >  2:102607/1‑93 (MQ=255)
                                                                     gggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAt                    <  1:5301113/93‑1 (MQ=255)
                                                                     gggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAt                    >  1:631781/1‑93 (MQ=255)
                                                                      ggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATg                   >  1:3829835/1‑93 (MQ=255)
                                                                        tGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCa                 <  2:2282533/93‑1 (MQ=255)
                                                                         gaagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCaa                <  1:3299753/93‑1 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               >  2:5295274/1‑93 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               >  1:1299829/1‑93 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              <  1:4197185/93‑1 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              >  2:1398851/1‑93 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             >  1:5733207/1‑93 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             >  2:3207494/1‑93 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  2:2620514/93‑1 (MQ=255)
                                                                             aaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAgg            >  2:5235113/1‑93 (MQ=255)
                                                                             aaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAgg            <  1:2070367/93‑1 (MQ=255)
                                                                               cTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGcc          <  2:2095886/93‑1 (MQ=255)
                                                                                ttACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         >  2:1897241/1‑93 (MQ=255)
                                                                                ttACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         <  2:3334657/93‑1 (MQ=255)
                                                                                  aCGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCgg       <  2:1228995/93‑1 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     <  1:725101/93‑1 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     >  2:5741677/1‑93 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     >  1:2652301/1‑93 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     <  1:2597693/93‑1 (MQ=255)
                                                                                     cagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAc    <  1:2752799/93‑1 (MQ=255)
                                                                                      agcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACg   <  1:5465993/93‑1 (MQ=255)
                                                                                             |                                                                                      
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  >  NC_000913/4103426‑4103612
                                                                                             |                                                                                              
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                <  SRR4302533.1578039/94‑1 (MQ=60)
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                <  SRR4302533.1973265/94‑1 (MQ=60)
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                <  SRR4302533.1842903/94‑1 (MQ=60)
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                <  SRR4302533.5552774/94‑1 (MQ=60)
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                >  SRR4302533.3763439/1‑94 (MQ=60)
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                <  SRR4302533.6176499/94‑1 (MQ=60)
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTAGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                <  SRR4302533.1316212/94‑1 (MQ=60)
 GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑T                                                                                              <  SRR4302533.6021931/94‑1 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              <  SRR4302533.125151/94‑3 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              >  SRR4302533.3739296/1‑92 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              >  SRR4302533.4441256/1‑92 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              <  SRR4302533.4497106/94‑3 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              <  SRR4302533.53690/94‑3 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              >  SRR4302533.1604829/1‑92 (MQ=60)
   CCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGtac                                                                                             <  SRR4302533.1543383/94‑4 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCATGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                            >  SRR4302533.4264446/1‑94 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                            >  SRR4302533.1617872/1‑94 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                            >  SRR4302533.5724211/1‑94 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                            >  SRR4302533.3890596/1‑94 (MQ=60)
     TCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGC                                                                                           >  SRR4302533.470607/1‑94 (MQ=60)
      CAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCA                                                                                          >  SRR4302533.3385773/1‑94 (MQ=60)
      CAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCA                                                                                          >  SRR4302533.637952/1‑94 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                         >  SRR4302533.5318613/1‑94 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGACGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGCACGCAG                                                                                         >  SRR4302533.279991/1‑94 (MQ=60)
         CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                       <  SRR4302533.3474471/94‑1 (MQ=60)
           CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                     >  SRR4302533.4070235/1‑94 (MQ=60)
           CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                     >  SRR4302533.2803610/1‑94 (MQ=60)
             GGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCAT                                                                                   >  SRR4302533.606003/1‑94 (MQ=60)
              GCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATA                                                                                  <  SRR4302533.2361989/94‑1 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                                 <  SRR4302533.5260214/94‑1 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                                 <  SRR4302533.1458326/94‑1 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                                 <  SRR4302533.6240603/94‑1 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                                <  SRR4302533.2663400/94‑1 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                                >  SRR4302533.770045/1‑94 (MQ=60)
                AGTCAGTTCATGAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGCTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                                <  SRR4302533.2591773/94‑1 (MQ=60)
                 GTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGT                                                                               <  SRR4302533.858572/94‑1 (MQ=60)
                  TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                              >  SRR4302533.2049653/1‑94 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             <  SRR4302533.4710472/94‑1 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             <  SRR4302533.3018695/94‑1 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             >  SRR4302533.6073425/1‑94 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             >  SRR4302533.3500550/1‑94 (MQ=60)
                     GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                           >  SRR4302533.2513231/1‑94 (MQ=60)
                      TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302533.3196991/94‑1 (MQ=60)
                      TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302533.311695/94‑1 (MQ=60)
                      TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302533.2132511/94‑1 (MQ=60)
                       TCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACG                                                                         >  SRR4302533.6127854/1‑94 (MQ=60)
                       TCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACG                                                                         >  SRR4302533.2125694/1‑94 (MQ=60)
                          TTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCA                                                                      >  SRR4302533.4763337/1‑94 (MQ=60)
                           TAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAT                                                                     <  SRR4302533.2155016/94‑1 (MQ=60)
                           TAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAT                                                                     <  SRR4302533.2742854/94‑1 (MQ=60)
                             AGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAA                                                                   <  SRR4302533.3771356/94‑1 (MQ=60)
                              GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                                  <  SRR4302533.4953701/94‑1 (MQ=60)
                              GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                                  <  SRR4302533.4953700/94‑1 (MQ=60)
                              GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                                  <  SRR4302533.5119466/94‑1 (MQ=60)
                               CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 >  SRR4302533.3193553/1‑94 (MQ=60)
                               CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 >  SRR4302533.3436503/1‑94 (MQ=60)
                                CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTT                                                                >  SRR4302533.4761911/1‑94 (MQ=60)
                                CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTT                                                                >  SRR4302533.1274277/1‑94 (MQ=60)
                                  CGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTA                                                              >  SRR4302533.5309416/1‑94 (MQ=60)
                                   GCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAA                                                             >  SRR4302533.4898243/1‑94 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            <  SRR4302533.2653341/94‑1 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            <  SRR4302533.5129410/94‑1 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            <  SRR4302533.3201959/94‑1 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            >  SRR4302533.6317112/1‑94 (MQ=60)
                                     TGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACC                                                           <  SRR4302533.2294828/94‑1 (MQ=60)
                                      GCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCG                                                          <  SRR4302533.4831969/94‑1 (MQ=60)
                                      GCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCG                                                          >  SRR4302533.3325144/1‑94 (MQ=60)
                                        TGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAA                                                        >  SRR4302533.6033617/1‑94 (MQ=60)
                                         GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                       >  SRR4302533.4594678/1‑94 (MQ=60)
                                           AGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                     <  SRR4302533.3175384/94‑1 (MQ=60)
                                            GTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATC                                                    <  SRR4302533.2185241/94‑1 (MQ=60)
                                             TCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCA                                                   <  SRR4302533.5050013/94‑1 (MQ=60)
                                             TCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCA                                                   <  SRR4302533.1004023/94‑1 (MQ=60)
                                             TCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCA                                                   <  SRR4302533.202936/94‑1 (MQ=60)
                                              CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                                  <  SRR4302533.4609495/94‑1 (MQ=60)
                                              CGGTTCAGGCGATAACTGCCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                                  <  SRR4302533.3835680/94‑1 (MQ=60)
                                               GGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGC                                                 <  SRR4302533.5914057/94‑1 (MQ=60)
                                               GGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGC                                                 >  SRR4302533.2616784/1‑94 (MQ=60)
                                                GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                                >  SRR4302533.4427953/1‑94 (MQ=60)
                                                GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                                <  SRR4302533.770902/94‑1 (MQ=60)
                                                  TCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTC                                              <  SRR4302533.3739583/94‑1 (MQ=60)
                                                   CAGGCGATAACTGGCATCCGCGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCA                                             <  SRR4302533.6165855/94‑1 (MQ=60)
                                                    AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                            <  SRR4302533.4703957/94‑1 (MQ=60)
                                                    AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                            >  SRR4302533.4593158/1‑94 (MQ=60)
                                                       CGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAG                                         <  SRR4302533.2768720/94‑1 (MQ=60)
                                                       CGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAG                                         >  SRR4302533.1629431/1‑94 (MQ=60)
                                                       CGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAG                                         >  SRR4302533.5890730/1‑94 (MQ=60)
                                                       CGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAG                                         <  SRR4302533.5675958/94‑1 (MQ=60)
                                                        GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                        >  SRR4302533.2924413/1‑94 (MQ=60)
                                                        GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                        <  SRR4302533.6253498/94‑1 (MQ=60)
                                                        GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                        >  SRR4302533.5902057/1‑94 (MQ=60)
                                                        GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                        <  SRR4302533.3550338/94‑1 (MQ=60)
                                                        GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                        <  SRR4302533.1086169/94‑1 (MQ=60)
                                                        GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                        >  SRR4302533.6140797/1‑94 (MQ=60)
                                                         ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       >  SRR4302533.1876215/1‑94 (MQ=60)
                                                         ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       <  SRR4302533.1974778/94‑1 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                      >  SRR4302533.5936113/1‑94 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                      <  SRR4302533.1071365/94‑1 (MQ=60)
                                                           AACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGC                                     >  SRR4302533.1871268/1‑94 (MQ=60)
                                                           AACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGC                                     >  SRR4302533.684064/1‑94 (MQ=60)
                                                            ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                    >  SRR4302533.2000820/1‑94 (MQ=60)
                                                              TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  >  SRR4302533.24211/1‑94 (MQ=60)
                                                              TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  >  SRR4302533.3519607/1‑94 (MQ=60)
                                                               GGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGA                                 <  SRR4302533.165638/94‑1 (MQ=60)
                                                                GCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAT                                <  SRR4302533.4571280/94‑1 (MQ=60)
                                                                 CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                               <  SRR4302533.2520937/94‑1 (MQ=60)
                                                                 CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                               >  SRR4302533.1926937/1‑94 (MQ=60)
                                                                  ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              >  SRR4302533.1282347/1‑94 (MQ=60)
                                                                  ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              >  SRR4302533.5709933/1‑94 (MQ=60)
                                                                  ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              <  SRR4302533.64694/94‑1 (MQ=60)
                                                                   TCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTT                             <  SRR4302533.248419/94‑1 (MQ=60)
                                                                     CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                           >  SRR4302533.635501/1‑94 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                          <  SRR4302533.6189694/94‑1 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                          >  SRR4302533.3853248/1‑94 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                          <  SRR4302533.5332872/94‑1 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                          <  SRR4302533.2883651/94‑1 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                          <  SRR4302533.3050777/94‑1 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        <  SRR4302533.5470639/94‑1 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTATAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        <  SRR4302533.1571159/94‑1 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCAACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        >  SRR4302533.189567/1‑94 (MQ=60)
                                                                         TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                       >  SRR4302533.5280756/1‑94 (MQ=60)
                                                                         TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                       >  SRR4302533.714903/1‑94 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      <  SRR4302533.3320117/94‑1 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      >  SRR4302533.1307650/1‑94 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      <  SRR4302533.5747231/94‑1 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      <  SRR4302533.4093183/94‑1 (MQ=60)
                                                                            AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    <  SRR4302533.4222594/94‑1 (MQ=60)
                                                                            AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    <  SRR4302533.4034030/94‑1 (MQ=60)
                                                                            AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    >  SRR4302533.5767820/1‑94 (MQ=60)
                                                                            AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    <  SRR4302533.5260418/94‑1 (MQ=60)
                                                                             GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                   >  SRR4302533.1773566/1‑94 (MQ=60)
                                                                              AACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGC                  <  SRR4302533.325902/94‑1 (MQ=60)
                                                                              AACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGC                  <  SRR4302533.2083118/94‑1 (MQ=60)
                                                                                 TTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCG               <  SRR4302533.518869/94‑1 (MQ=60)
                                                                                 TTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCG               >  SRR4302533.2329771/1‑94 (MQ=60)
                                                                                  TACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGG              <  SRR4302533.2635406/94‑1 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA            >  SRR4302533.2668596/1‑94 (MQ=60)
                                                                                     GCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAC           <  SRR4302533.2613655/94‑1 (MQ=60)
                                                                                     GCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAC           <  SRR4302533.729389/94‑1 (MQ=60)
                                                                                      CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG          <  SRR4302533.2769679/94‑1 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA         <  SRR4302533.5498937/94‑1 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA         <  SRR4302533.1645810/94‑1 (MQ=60)
                                                                                        GCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAA        >  SRR4302533.34691/1‑94 (MQ=60)
                                                                                         CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC       <  SRR4302533.758765/94‑1 (MQ=60)
                                                                                         CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC       >  SRR4302533.2870855/1‑94 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      >  SRR4302533.2522407/1‑94 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      <  SRR4302533.4842836/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      <  SRR4302533.3618460/94‑1 (MQ=60)
                                                                                           CAGTACGCCGAGCCATATGTGCGACGGTATAAGTTTAACCAAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     <  SRR4302533.1724325/94‑1 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     <  SRR4302533.1092524/94‑1 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     >  SRR4302533.5611564/1‑94 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAC     >  SRR4302533.5119160/1‑94 (MQ=60)
                                                                                             T‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  <  SRR4302533.3806787/94‑1 (MQ=60)
                                                                                             |                                                                                              
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  >  NC_000913/4103426‑4103612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑