Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,107,129 A→G E239G (GAG→GGG)  sbp → periplasmic sulfate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,107,1290AG99.6% 815.4 / NA 258E239G (GAG→GGG) sbpperiplasmic sulfate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (143/114);  total (144/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 266 total aligned reads displayed.

TTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCA  >  NC_000913/4107039‑4107217
                                                                                          |                                                                                        
ttGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGc                                                                                        >  2:4587671/1‑93 (MQ=255)
   ccTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGCGAGTCTATCCTCGCAGGGCCaa                                                                                     >  2:3131364/1‑93 (MQ=255)
    cTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAAc                                                                                    <  1:2564657/93‑1 (MQ=255)
    cTGGGAAAACGAAGCTCTGCGGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAAc                                                                                    <  1:1390028/93‑1 (MQ=255)
     tGGGAAAACGAAGCTCTGCTGGCAGCGAATGGACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAAcc                                                                                   >  2:155902/1‑93 (MQ=255)
     tGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAAcc                                                                                   <  1:2822128/93‑1 (MQ=255)
     tGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAAcc                                                                                   >  2:3794884/1‑93 (MQ=255)
      gggAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCg                                                                                  >  1:2912446/1‑93 (MQ=255)
       ggAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCgt                                                                                 <  1:1183075/93‑1 (MQ=255)
       ggAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCgt                                                                                 >  1:3865216/1‑93 (MQ=255)
         aaaaCGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAAGCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCgtgt                                                                               <  1:1049863/93‑1 (MQ=255)
          aaaCGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTc                                                                              <  1:4223598/93‑1 (MQ=255)
             cGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCggt                                                                           <  1:1703887/93‑1 (MQ=255)
               aaGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCggtgg                                                                         <  2:3577662/93‑1 (MQ=255)
                aGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCggtggt                                                                        <  2:2070676/93‑1 (MQ=255)
                 gCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTc                                                                       <  2:797356/93‑1 (MQ=255)
                 gCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTc                                                                       <  1:339419/93‑1 (MQ=255)
                   tctGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGa                                                                     >  1:5071110/1‑93 (MQ=255)
                   tctGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGa                                                                     <  2:5887000/93‑1 (MQ=255)
                    ctgctgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGAt                                                                    <  1:5933852/93‑1 (MQ=255)
                       ctgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATaaa                                                                 >  1:1565662/1‑93 (MQ=255)
                       ctgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATaaa                                                                 <  2:6182181/93‑1 (MQ=255)
                       ctgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGGGTCGGTGGTCGATacg                                                                 >  2:3409165/1‑91 (MQ=255)
                        tgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAg                                                                >  2:2905746/1‑93 (MQ=255)
                        tgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAg                                                                <  1:5732868/93‑1 (MQ=255)
                         ggCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGt                                                               <  2:3346413/93‑1 (MQ=255)
                         ggCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGt                                                               >  1:2092472/1‑93 (MQ=255)
                          gCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTg                                                              <  1:568322/93‑1 (MQ=255)
                          gCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTg                                                              >  1:5268175/1‑93 (MQ=255)
                           cAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTgg                                                             <  2:3008360/93‑1 (MQ=255)
                            aGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGt                                                            <  1:6034361/93‑1 (MQ=255)
                            aGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGt                                                            <  2:4238553/93‑1 (MQ=255)
                             gCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTc                                                           >  1:2396927/1‑93 (MQ=255)
                             gCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTc                                                           <  1:4016830/93‑1 (MQ=255)
                               gAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCga                                                         >  2:887209/1‑93 (MQ=255)
                               gAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCga                                                         >  2:3844884/1‑93 (MQ=255)
                                aaTGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCgag                                                        >  1:3532378/1‑93 (MQ=255)
                                aaTGAACTGGGCAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCgag                                                        <  2:1648916/93‑1 (MQ=255)
                                 aTGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCgaga                                                       >  1:3113660/1‑93 (MQ=255)
                                 aTGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCgaga                                                       >  1:840113/1‑93 (MQ=255)
                                  tGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGaa                                                      <  2:4461157/93‑1 (MQ=255)
                                    aaCTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGaaaa                                                    >  1:5856348/1‑93 (MQ=255)
                                     aCTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGaaaaa                                                   >  2:4608122/1‑93 (MQ=255)
                                      cTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGaaaaaa                                                  <  1:502792/93‑1 (MQ=255)
                                       tGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAg                                                 >  2:4772602/1‑93 (MQ=255)
                                       tGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAg                                                 >  2:4933955/1‑93 (MQ=255)
                                        ggggAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAgg                                                <  1:4464869/93‑1 (MQ=255)
                                        ggggAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAgg                                                >  1:1611543/1‑93 (MQ=255)
                                         gggAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGt                                               <  2:1563673/93‑1 (MQ=255)
                                          ggAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTa                                              >  1:615164/1‑93 (MQ=255)
                                          ggAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTa                                              >  2:3600493/1‑93 (MQ=255)
                                           gAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTAc                                             >  2:832941/1‑93 (MQ=255)
                                           gAAAGATAAATGCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTAc                                             <  1:887514/93‑1 (MQ=255)
                                               gATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTaaa                                         >  2:5771157/1‑93 (MQ=255)
                                                aTAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAag                                        <  1:1822664/93‑1 (MQ=255)
                                                 tAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAaga                                       >  2:2856882/1‑93 (MQ=255)
                                                 tAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAaga                                       <  1:3342179/93‑1 (MQ=255)
                                                 tAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAaga                                       >  2:387702/1‑93 (MQ=255)
                                                 tAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAaga                                       >  2:4825428/1‑93 (MQ=255)
                                                  aaaTTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAagag                                      >  1:2308929/1‑93 (MQ=255)
                                                   aaTTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAgg                                     <  2:5244847/93‑1 (MQ=255)
                                                   aaTTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAgg                                     <  1:3831381/93‑1 (MQ=255)
                                                     ttCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTg                                   <  1:2201260/93‑1 (MQ=255)
                                                     ttCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTg                                   >  2:177895/1‑93 (MQ=255)
                                                      tCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTgg                                  <  2:1535133/93‑1 (MQ=255)
                                                       cGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGc                                 <  1:1548036/93‑1 (MQ=255)
                                                        gAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCg                                <  2:2715104/93‑1 (MQ=255)
                                                        gAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCg                                <  2:1317474/93‑1 (MQ=255)
                                                        gAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCg                                >  1:2652816/1‑93 (MQ=255)
                                                           aTCGGCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGaa                             <  1:4949765/93‑1 (MQ=255)
                                                            tCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAg                            >  2:1273395/1‑93 (MQ=255)
                                                             cGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGc                           >  2:5428536/1‑93 (MQ=255)
                                                              gTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGcc                          >  1:4909949/1‑93 (MQ=255)
                                                              gTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGcc                          <  2:5746534/93‑1 (MQ=255)
                                                               tCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCt                         >  2:3679823/1‑93 (MQ=255)
                                                               tCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCt                         >  1:483603/1‑93 (MQ=255)
                                                               tCACGCCGAGTGAGTCAATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCt                         <  2:5025696/93‑1 (MQ=255)
                                                                 aCGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTAc                       >  2:4511752/1‑93 (MQ=255)
                                                                 aCGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTAc                       >  2:1967486/1‑93 (MQ=255)
                                                                  cGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTAcc                      <  2:4102956/93‑1 (MQ=255)
                                                                  cGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTAcc                      <  1:1260553/93‑1 (MQ=255)
                                                                  cGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTAcc                      >  2:1213064/1‑93 (MQ=255)
                                                                  cGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACAAAAGAGGTGGCGGAAGCCTAcc                      <  1:3997274/93‑1 (MQ=255)
                                                                     cGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAGGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGa                   <  2:5073582/93‑1 (MQ=255)
                                                                     cGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGa                   >  1:4059983/1‑93 (MQ=255)
                                                                      gagtgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGaa                  >  2:5599874/1‑93 (MQ=255)
                                                                      gagtgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGaa                  >  2:1025998/1‑93 (MQ=255)
                                                                      gagtgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGaa                  >  2:321645/1‑93 (MQ=255)
                                                                       agtgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGaaa                 <  1:3279392/93‑1 (MQ=255)
                                                                       agtgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGaaa                 >  2:5050198/1‑93 (MQ=255)
                                                                       agtgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGaaa                 >  1:3674166/1‑93 (MQ=255)
                                                                        gtgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAat                >  2:618535/1‑93 (MQ=255)
                                                                         tgagtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAata               <  2:3125199/93‑1 (MQ=255)
                                                                            gtCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATAtct            <  1:436609/93‑1 (MQ=255)
                                                                             tCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATAtctc           <  1:1016445/93‑1 (MQ=255)
                                                                               tATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTa         >  2:220920/1‑93 (MQ=255)
                                                                               tATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTa         >  2:67456/1‑93 (MQ=255)
                                                                                aTCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTAc        >  2:4655742/1‑93 (MQ=255)
                                                                                    tCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGc    <  2:1848528/93‑1 (MQ=255)
                                                                                      gCAGGGCAAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCa  <  2:1519079/93‑1 (MQ=255)
                                                                                          |                                                                                        
TTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCA  >  NC_000913/4107039‑4107217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGG  >  NC_000913/4107036‑4107222
                                                                                             |                                                                                             
TGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGG                                                                                               >  SRR4302533.2243370/1‑94 (MQ=60)
TGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGG                                                                                               >  SRR4302533.2932001/1‑94 (MQ=60)
TGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGG                                                                                               >  SRR4302533.1605635/1‑94 (MQ=60)
TGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGG                                                                                               >  SRR4302533.4517857/1‑94 (MQ=60)
  ATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGC                                                                                             <  SRR4302533.2284926/94‑1 (MQ=60)
  ATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGC                                                                                             >  SRR4302533.1338314/1‑94 (MQ=60)
  ATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGC                                                                                             >  SRR4302533.3997244/1‑94 (MQ=60)
       CTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACC                                                                                        >  SRR4302533.5490806/1‑94 (MQ=60)
       CTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACC                                                                                        <  SRR4302533.2580439/94‑1 (MQ=60)
       CTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACC                                                                                        >  SRR4302533.2889377/1‑94 (MQ=60)
       CTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACC                                                                                        <  SRR4302533.4055578/94‑1 (MQ=60)
       CTGGGAAAACGAAGCTCTGCGGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACC                                                                                        <  SRR4302533.1398400/94‑1 (MQ=60)
        TGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCG                                                                                       <  SRR4302533.2839497/94‑1 (MQ=60)
        TGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCG                                                                                       >  SRR4302533.2930321/1‑94 (MQ=60)
         GGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGT                                                                                      >  SRR4302533.3888812/1‑94 (MQ=60)
          GGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTG                                                                                     <  SRR4302533.1190181/94‑1 (MQ=60)
            AAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTC                                                                                   >  SRR4302533.1084445/1‑94 (MQ=60)
            AAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTC                                                                                   >  SRR4302533.2150594/1‑94 (MQ=60)
            AAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAAGCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTC                                                                                   <  SRR4302533.1056035/94‑1 (MQ=60)
             AAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCG                                                                                  <  SRR4302533.4249133/94‑1 (MQ=60)
             AAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCG                                                                                  >  SRR4302533.5189968/1‑94 (MQ=60)
             AAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCG                                                                                  <  SRR4302533.2893364/94‑1 (MQ=60)
             AAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCG                                                                                  >  SRR4302533.331741/1‑94 (MQ=60)
                CGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTG                                                                               >  SRR4302533.3329171/1‑94 (MQ=60)
                CGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTG                                                                               >  SRR4302533.2935414/1‑94 (MQ=60)
                CGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTG                                                                               <  SRR4302533.1714208/94‑1 (MQ=60)
                CGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTG                                                                               >  SRR4302533.3785891/1‑94 (MQ=60)
                CGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTG                                                                               <  SRR4302533.4074292/94‑1 (MQ=60)
                CGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACCACGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTG                                                                               >  SRR4302533.2696810/1‑94 (MQ=60)
                 GAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGG                                                                              <  SRR4302533.1685825/94‑1 (MQ=60)
                   AGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTC                                                                            <  SRR4302533.1838070/94‑1 (MQ=60)
                   AGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTC                                                                            >  SRR4302533.4024149/1‑94 (MQ=60)
                    GCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCG                                                                           <  SRR4302533.341489/94‑1 (MQ=60)
                    GCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCG                                                                           <  SRR4302533.4986110/94‑1 (MQ=60)
                    GCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCG                                                                           <  SRR4302533.1572170/94‑1 (MQ=60)
                     CTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGA                                                                          >  SRR4302533.5101763/1‑94 (MQ=60)
                      TCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGAT                                                                         >  SRR4302533.2408703/1‑94 (MQ=60)
                       CTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATA                                                                        <  SRR4302533.5969725/94‑1 (MQ=60)
                         GCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAA                                                                      >  SRR4302533.5987679/1‑94 (MQ=60)
                         GCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAA                                                                      >  SRR4302533.6323946/1‑94 (MQ=60)
                         GCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAA                                                                      >  SRR4302533.1575090/1‑94 (MQ=60)
                          CTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAG                                                                     >  SRR4302533.905515/1‑94 (MQ=60)
                           TGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGT                                                                    <  SRR4302533.5767480/94‑1 (MQ=60)
                           TGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGT                                                                    <  SRR4302533.4919162/94‑1 (MQ=60)
                           TGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGT                                                                    >  SRR4302533.2105358/1‑94 (MQ=60)
                            GGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTG                                                                   >  SRR4302533.5299778/1‑94 (MQ=60)
                             GCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGG                                                                  <  SRR4302533.571682/94‑1 (MQ=60)
                             GCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGG                                                                  <  SRR4302533.4009200/94‑1 (MQ=60)
                              CAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGT                                                                 <  SRR4302533.1229005/94‑1 (MQ=60)
                               AGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTC                                                                >  SRR4302533.2411723/1‑94 (MQ=60)
                               AGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTC                                                                <  SRR4302533.6070929/94‑1 (MQ=60)
                                GCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCG                                                               <  SRR4302533.4041288/94‑1 (MQ=60)
                                 CGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGA                                                              >  SRR4302533.2613334/1‑94 (MQ=60)
                                  GAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAG                                                             >  SRR4302533.3554145/1‑94 (MQ=60)
                                  GAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAG                                                             >  SRR4302533.3860003/1‑94 (MQ=60)
                                  GAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAG                                                             <  SRR4302533.1603886/94‑1 (MQ=60)
                                  GAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAG                                                             >  SRR4302533.2516136/1‑94 (MQ=60)
                                   AATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGA                                                            >  SRR4302533.3132809/1‑94 (MQ=60)
                                   AATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGA                                                            >  SRR4302533.845027/1‑94 (MQ=60)
                                   AATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGA                                                            >  SRR4302533.5175844/1‑94 (MQ=60)
                                    ATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAA                                                           <  SRR4302533.856701/94‑1 (MQ=60)
                                      GAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAA                                                         >  SRR4302533.5899872/1‑94 (MQ=60)
                                      GAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAA                                                         >  SRR4302533.5891752/1‑94 (MQ=60)
                                       AACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAA                                                        <  SRR4302533.1455344/94‑1 (MQ=60)
                                        ACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAA                                                       >  SRR4302533.5095395/1‑94 (MQ=60)
                                         CTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAG                                                      <  SRR4302533.505781/94‑1 (MQ=60)
                                         CTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGCGAGTCTAGCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAG                                                      >  SRR4302533.3411832/1‑94 (MQ=60)
                                          TGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATTAAGTGGTCGAGAAAAAAGG                                                     >  SRR4302533.5828301/1‑94 (MQ=60)
                                          TGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGG                                                     >  SRR4302533.4122924/1‑94 (MQ=60)
                                          TGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGG                                                     >  SRR4302533.3199538/1‑94 (MQ=60)
                                          TGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGG                                                     >  SRR4302533.1621314/1‑94 (MQ=60)
                                          TGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGG                                                     <  SRR4302533.3551276/94‑1 (MQ=60)
                                           GGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGT                                                    <  SRR4302533.4491874/94‑1 (MQ=60)
                                            GGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTA                                                   >  SRR4302533.618778/1‑94 (MQ=60)
                                             GGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTAC                                                  <  SRR4302533.3637387/94‑1 (MQ=60)
                                              GAAAGATAAATGCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACT                                                 <  SRR4302533.892723/94‑1 (MQ=60)
                                               AAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTA                                                >  SRR4302533.1179174/1‑94 (MQ=60)
                                               AAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTA                                                >  SRR4302533.3212882/1‑94 (MQ=60)
                                                 AGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAA                                              >  SRR4302533.5745851/1‑94 (MQ=60)
                                                  GATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAG                                             <  SRR4302533.814421/94‑1 (MQ=60)
                                                  GATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAG                                             >  SRR4302533.447017/1‑94 (MQ=60)
                                                  GATAAATTCGAAATCGTCACGCCGAGTCAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAG                                             >  SRR4302533.5049995/1‑94 (MQ=60)
                                                   ATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGA                                            <  SRR4302533.1833797/94‑1 (MQ=60)
                                                    TAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAG                                           <  SRR4302533.3362837/94‑1 (MQ=60)
                                                    TAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAG                                           >  SRR4302533.2323233/1‑94 (MQ=60)
                                                     AAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGG                                          >  SRR4302533.4407102/1‑94 (MQ=60)
                                                      AATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGT                                         <  SRR4302533.3854803/94‑1 (MQ=60)
                                                      AATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGG                                         >  SRR4302533.385611/1‑94 (MQ=60)
                                                        TTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGG                                       <  SRR4302533.2214823/94‑1 (MQ=60)
                                                          CGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCG                                     <  SRR4302533.1557364/94‑1 (MQ=60)
                                                          CGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCG                                     >  SRR4302533.5939942/1‑94 (MQ=60)
                                                          CGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCG                                     >  SRR4302533.2669114/1‑94 (MQ=60)
                                                             AATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAA                                  <  SRR4302533.2787271/94‑1 (MQ=60)
                                                              ATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAG                                 >  SRR4302533.893461/1‑94 (MQ=60)
                                                              ATCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAG                                 >  SRR4302533.1844237/1‑94 (MQ=60)
                                                              ATCGGCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAG                                 <  SRR4302533.4979745/94‑1 (MQ=60)
                                                               TCGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGC                                <  SRR4302533.613474/94‑1 (MQ=60)
                                                                CGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCC                               <  SRR4302533.5798796/94‑1 (MQ=60)
                                                                CGTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCC                               >  SRR4302533.4939692/1‑94 (MQ=60)
                                                                 GTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCT                              >  SRR4302533.486491/1‑94 (MQ=60)
                                                                 GTCACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCT                              >  SRR4302533.4331714/1‑94 (MQ=60)
                                                                   CACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTAC                            <  SRR4302533.1588369/94‑1 (MQ=60)
                                                                    ACGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACC                           <  SRR4302533.1505324/94‑1 (MQ=60)
                                                                     CGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCT                          <  SRR4302533.5611601/94‑1 (MQ=60)
                                                                     CGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCT                          <  SRR4302533.1268093/94‑1 (MQ=60)
                                                                     CGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCT                          >  SRR4302533.1355974/1‑94 (MQ=60)
                                                                     CGCCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACAAAAGAGGTGGCGGAAGCCTACCT                          <  SRR4302533.4021614/94‑1 (MQ=60)
                                                                       CCGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGA                        >  SRR4302533.4084695/1‑94 (MQ=60)
                                                                        CGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAA                       >  SRR4302533.3439073/1‑94 (MQ=60)
                                                                        CGAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAA                       >  SRR4302533.4647231/1‑94 (MQ=60)
                                                                         GAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAA                      >  SRR4302533.3696748/1‑94 (MQ=60)
                                                                         GAGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAA                      >  SRR4302533.6109902/1‑94 (MQ=60)
                                                                         GAGTGAGTCTATCCTCACAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAA                      >  SRR4302533.168200/1‑94 (MQ=60)
                                                                          AGTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAAT                     <  SRR4302533.3299638/94‑1 (MQ=60)
                                                                           GTGAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATA                    <  SRR4302533.173488/94‑1 (MQ=60)
                                                                             GAGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATC                  <  SRR4302533.2133877/94‑1 (MQ=60)
                                                                              AGTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCT                 >  SRR4302533.4963531/1‑94 (MQ=60)
                                                                               GTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTC                <  SRR4302533.3587735/94‑1 (MQ=60)
                                                                               GTCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTC                <  SRR4302533.439210/94‑1 (MQ=60)
                                                                                TCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCT               <  SRR4302533.1022419/94‑1 (MQ=60)
                                                                                TCTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCT               >  SRR4302533.2264686/1‑94 (MQ=60)
                                                                                 CTATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTA              >  SRR4302533.4942142/1‑94 (MQ=60)
                                                                                   ATCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAGAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACT            >  SRR4302533.4114171/1‑94 (MQ=60)
                                                                                    TCCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTC           <  SRR4302533.4615461/94‑1 (MQ=60)
                                                                                     CCTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCG          >  SRR4302533.2385339/1‑94 (MQ=60)
                                                                                      CTCGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGC         >  SRR4302533.2486341/1‑94 (MQ=60)
                                                                                        CGCGGGGCCACCCGTGTCGGTGGTAGATAAAGTGGTCGAGAAAAAAGTTGCTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCA       <  SRR4302533.2207440/94‑1 (MQ=60)
                                                                                        CGCAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCA       >  SRR4302533.1031278/1‑94 (MQ=60)
                                                                                          CAGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGA     >  SRR4302533.4424581/1‑94 (MQ=60)
                                                                                           AGGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAA    <  SRR4302533.1123801/94‑1 (MQ=60)
                                                                                            GGGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAG   <  SRR4302533.1670343/94‑1 (MQ=60)
                                                                                             GGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTCCTCGCCAGAAGG  >  SRR4302533.4974620/1‑94 (MQ=60)
                                                                                             GGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGG  >  SRR4302533.746106/1‑94 (MQ=60)
                                                                                             GGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGG  <  SRR4302533.1136147/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGG  >  NC_000913/4107036‑4107222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑