Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,134,509 A→G E274E (GAA→GAG)  b3943 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,134,5090AG99.4% 566.5 / ‑4.9 176E274E (GAA→GAG) b3943hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (69/106);  total (70/106)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 190 total aligned reads displayed.

CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598
                                                                                          |                                                                                         
cAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAggg                                                                                         <  1:5542629/93‑1 (MQ=255)
 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        <  2:2530198/93‑1 (MQ=255)
  gTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTa                                                                                       <  1:5305727/93‑1 (MQ=255)
   tATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAg                                                                                      >  1:5127297/1‑93 (MQ=255)
   tATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAg                                                                                      <  2:3533057/93‑1 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     >  2:4200983/1‑93 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     <  1:4037329/93‑1 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     >  2:289559/1‑93 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     >  2:5941790/1‑93 (MQ=255)
      tGCCAGCTTAAGCACACTGTCCGTTCCCGTTTGCGGCGTCCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCgg                                                                                   <  1:803193/93‑1 (MQ=255)
      tGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCgg                                                                                   <  2:2265966/93‑1 (MQ=255)
        ccAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCggga                                                                                 >  2:2689023/1‑91 (MQ=255)
         cAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTat                                                                                >  1:2044719/1‑93 (MQ=255)
          aGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTCCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTatt                                                                               >  2:4312603/1‑93 (MQ=255)
           gCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattg                                                                              >  2:1172863/1‑93 (MQ=255)
            cTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgt                                                                             <  2:2091089/93‑1 (MQ=255)
              tAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgc                                                                           <  1:3378538/93‑1 (MQ=255)
              tAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgc                                                                           >  2:903956/1‑93 (MQ=255)
               aaGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgct                                                                          >  1:379427/1‑93 (MQ=255)
                 gCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctga                                                                        <  2:1610319/93‑1 (MQ=255)
                  cacaCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgat                                                                       <  1:3904359/93‑1 (MQ=255)
                       tGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgc                                                                  >  1:5761205/1‑93 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 >  1:2586456/1‑93 (MQ=255)
                          ccGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTgg                                                               <  2:4229298/93‑1 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              >  1:4404000/1‑93 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              >  2:3377746/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:3178827/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  1:2824915/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:4758740/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:73387/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:7846/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            <  1:1272218/93‑1 (MQ=255)
                               cccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTg                                                          <  2:1398131/93‑1 (MQ=255)
                               cccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTg                                                          >  1:5468159/1‑93 (MQ=255)
                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                                         <  2:28384/93‑1 (MQ=255)
                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACCgg                                                         <  1:2555717/93‑1 (MQ=255)
                                  gTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgcg                                                       >  1:1680129/1‑93 (MQ=255)
                                    ttGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCt                                                     <  1:2409776/93‑1 (MQ=255)
                                     tGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTg                                                    <  2:2310516/93‑1 (MQ=255)
                                      gcggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTgg                                                   <  1:4362966/93‑1 (MQ=255)
                                      gcggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTgg                                                   <  1:4552123/93‑1 (MQ=255)
                                       gggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGt                                                  <  1:3311965/92‑1 (MQ=255)
                                        ggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 >  1:6054405/1‑93 (MQ=255)
                                        cgcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 <  1:5937848/92‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:4686040/93‑1 (MQ=255)
                                           gTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGc                                              <  2:852352/93‑1 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  2:3169889/1‑93 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             <  2:2368144/93‑1 (MQ=255)
                                             aCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCg                                            >  1:457093/1‑93 (MQ=255)
                                             aCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCg                                            <  2:4353136/93‑1 (MQ=255)
                                              ctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGt                                           >  1:2119658/1‑93 (MQ=255)
                                              ctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGt                                           <  2:5333538/93‑1 (MQ=255)
                                               tctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTa                                          >  2:3843688/1‑93 (MQ=255)
                                                ctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTaa                                         <  2:2075229/93‑1 (MQ=255)
                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        <  1:1577147/93‑1 (MQ=255)
                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        >  1:4868124/1‑93 (MQ=255)
                                                  tttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGa                                       <  1:2888876/93‑1 (MQ=255)
                                                  tttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGa                                       <  2:5003297/93‑1 (MQ=255)
                                                   ttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaa                                      <  1:929501/93‑1 (MQ=255)
                                                    tCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaa                                     <  1:1189493/93‑1 (MQ=255)
                                                    tCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaa                                     >  1:6174626/1‑93 (MQ=255)
                                                     ccTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaa                                    <  2:2118005/93‑1 (MQ=255)
                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   <  2:1109588/93‑1 (MQ=255)
                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   <  1:5371166/93‑1 (MQ=255)
                                                       tggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaaa                                  >  1:1110659/1‑93 (MQ=255)
                                                        ggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaag                                 <  1:4112168/93‑1 (MQ=255)
                                                          tggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa                               <  1:2263337/93‑1 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAACAAGAAGc                             >  1:179543/1‑93 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  1:1913296/93‑1 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  1:1721170/93‑1 (MQ=255)
                                                             ctgctCGGCGAGAGTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            <  1:127684/93‑1 (MQ=255)
                                                             ctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            <  2:6109192/93‑1 (MQ=255)
                                                             ctgctAGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            <  2:5763472/93‑1 (MQ=255)
                                                              tgctCGGCGAGAATCCGGGGGCCGTTGAGGTTAGCGGTATTGTGCTGATTGTGCTGGCACGGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCg                           <  1:3841969/93‑1 (MQ=255)
                                                               gctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt                          <  2:3241004/93‑1 (MQ=255)
                                                               gctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt                          <  1:2619075/93‑1 (MQ=255)
                                                                ctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc                         <  2:4171581/93‑1 (MQ=255)
                                                                ctCAGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc                         <  1:6300140/93‑1 (MQ=255)
                                                                 tgggCGAGAATCCGGGGGCTGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  2:1788810/91‑1 (MQ=255)
                                                                 tCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  2:1874848/93‑1 (MQ=255)
                                                                 gcGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  1:4982768/92‑1 (MQ=255)
                                                                        gaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaa                 <  1:852062/93‑1 (MQ=255)
                                                                          aTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaaa               >  1:5229891/1‑93 (MQ=255)
                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  1:3043353/93‑1 (MQ=255)
                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              >  1:2629813/1‑93 (MQ=255)
                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  2:286397/93‑1 (MQ=255)
                                                                            ccGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAgg             <  2:4858479/93‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  2:4159076/93‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            >  1:5603255/1‑93 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  1:5842155/93‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  1:2056877/93‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  2:28188/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          >  2:4096378/1‑93 (MQ=255)
                                                                                gggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCt         <  2:926867/93‑1 (MQ=255)
                                                                                 ggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTg        <  2:1825808/93‑1 (MQ=255)
                                                                                  gCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGa       >  1:6042067/1‑93 (MQ=255)
                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     <  2:2449549/93‑1 (MQ=255)
                                                                                     gTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAtt    <  1:2439103/93‑1 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  1:1964121/1‑93 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  2:454337/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  >  NC_000913/4134416‑4134602
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               <  SRR4302533.562214/94‑1 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             <  SRR4302533.1581443/94‑1 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             <  SRR4302533.2630843/94‑1 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             <  SRR4302533.4454153/94‑1 (MQ=60)
   CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGT                                                                                            <  SRR4302533.5576104/94‑1 (MQ=60)
   CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGT                                                                                            <  SRR4302533.2040809/94‑1 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          >  SRR4302533.5158272/1‑94 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          <  SRR4302533.5337523/94‑1 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        <  SRR4302533.4061899/94‑1 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        <  SRR4302533.1804195/94‑1 (MQ=60)
        TTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGG                                                                                       <  SRR4302533.392448/94‑1 (MQ=60)
         TGCCAGCTTAAGCACACTGTCCGTTCCCGTTTGCGGCGTCCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGT                                                                                      <  SRR4302533.807910/94‑1 (MQ=60)
           CCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTAT                                                                                    >  SRR4302533.2057324/1‑94 (MQ=60)
            CAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATT                                                                                   >  SRR4302533.5189114/1‑94 (MQ=60)
                 TAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCT                                                                              >  SRR4302533.381713/1‑94 (MQ=60)
                 TAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCT                                                                              <  SRR4302533.3399396/94‑1 (MQ=60)
                     CACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATT                                                                          <  SRR4302533.3928204/94‑1 (MQ=60)
                       CACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGT                                                                        <  SRR4302533.3986588/94‑1 (MQ=60)
                         CTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGC                                                                      >  SRR4302533.2578121/1‑94 (MQ=60)
                         CTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGC                                                                      >  SRR4302533.5795967/1‑94 (MQ=60)
                          TGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCT                                                                     >  SRR4302533.2602357/1‑94 (MQ=60)
                          TGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCG                                                                     >  SRR4302533.6258379/1‑94 (MQ=60)
                            GCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGG                                                                   >  SRR4302533.4839062/1‑94 (MQ=60)
                             CCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGC                                                                  >  SRR4302533.4430593/1‑94 (MQ=60)
                              CGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCA                                                                 <  SRR4302533.139859/94‑1 (MQ=60)
                              CGTTCCCGTTTGCGGCGTAATCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGGCGTTGAGGGTAGCGGTGTTGTGATCATTGTGCTGGCA                                                                 >  SRR4302533.1173728/1‑94 (MQ=60)
                               GTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAC                                                                >  SRR4302533.2842299/1‑94 (MQ=60)
                                TTTCCGTTTGCGGCGTGCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACT                                                               <  SRR4302533.2468188/94‑1 (MQ=60)
                                TTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACT                                                               <  SRR4302533.1279828/94‑1 (MQ=60)
                                TTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACT                                                               >  SRR4302533.4582788/1‑94 (MQ=60)
                                 TCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTG                                                              >  SRR4302533.5501114/1‑94 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACCGGC                                                            <  SRR4302533.2571437/94‑1 (MQ=60)
                                    CGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCG                                                           >  SRR4302533.1690307/1‑94 (MQ=60)
                                     GTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGTACTGGCGC                                                          >  SRR4302533.5799836/1‑94 (MQ=60)
                                       TTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTG                                                        <  SRR4302533.2424659/94‑1 (MQ=60)
                                         GCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGT                                                      <  SRR4302533.4389304/94‑1 (MQ=60)
                                         GCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGT                                                      <  SRR4302533.1281421/94‑1 (MQ=60)
                                         GCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGT                                                      <  SRR4302533.4579730/94‑1 (MQ=60)
                                          GGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTG                                                     <  SRR4302533.3332418/94‑1 (MQ=60)
                                          CGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTG                                                     >  SRR4302533.6091070/1‑94 (MQ=60)
                                           GGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGA                                                    <  SRR4302533.2250876/94‑1 (MQ=60)
                                           CGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGA                                                    <  SRR4302533.5973748/94‑1 (MQ=60)
                                            GCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAG                                                   <  SRR4302533.744687/94‑1 (MQ=60)
                                               TACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCG                                                >  SRR4302533.459827/1‑94 (MQ=60)
                                               TACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCG                                                >  SRR4302533.598951/1‑94 (MQ=60)
                                                ACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGT                                               >  SRR4302533.2132732/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302533.16840/1‑94 (MQ=60)
                                                  TCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAA                                             >  SRR4302533.3067054/1‑94 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            >  SRR4302533.4897623/1‑94 (MQ=60)
                                                    TTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGA                                           >  SRR4302533.2380329/1‑94 (MQ=60)
                                                    TTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGA                                           <  SRR4302533.1586644/94‑1 (MQ=60)
                                                     TTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAA                                          <  SRR4302533.2906634/94‑1 (MQ=60)
                                                     TTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAA                                          >  SRR4302533.2638836/1‑94 (MQ=60)
                                                      TTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAA                                         >  SRR4302533.6211730/1‑94 (MQ=60)
                                                      TTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAA                                         <  SRR4302533.934956/94‑1 (MQ=60)
                                                       TCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAA                                        <  SRR4302533.1196633/94‑1 (MQ=60)
                                                         CTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAA                                      >  SRR4302533.1117248/1‑94 (MQ=60)
                                                         CTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAA                                      <  SRR4302533.5403437/94‑1 (MQ=60)
                                                          TGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAG                                     <  SRR4302533.5654034/94‑1 (MQ=60)
                                                           GGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGA                                    <  SRR4302533.4137162/94‑1 (MQ=60)
                                                           GGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGA                                    >  SRR4302533.2428307/1‑94 (MQ=60)
                                                           GGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGA                                    <  SRR4302533.6085682/94‑1 (MQ=60)
                                                            GTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAA                                   >  SRR4302533.926999/1‑94 (MQ=60)
                                                            GTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAA                                   <  SRR4302533.5628771/94‑1 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  >  SRR4302533.6123142/1‑94 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  <  SRR4302533.2277342/94‑1 (MQ=60)
                                                              GGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAACAAGAAGC                                 >  SRR4302533.180667/1‑94 (MQ=60)
                                                              GGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGC                                 <  SRR4302533.292062/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302533.5470863/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302533.1731608/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302533.1924971/94‑1 (MQ=60)
                                                                CTGCTCGGCGAGAGTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCG                               <  SRR4302533.128482/94‑1 (MQ=60)
                                                                 TGCTCGGCGAGAATCCGGGGGCCGTTGAGGTTAGCGGTATTGTGCTGATTGTGCTGGCACGGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGT                              <  SRR4302533.3865441/94‑1 (MQ=60)
                                                                  GCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC                             <  SRR4302533.2635153/94‑1 (MQ=60)
                                                                  GCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC                             <  SRR4302533.5893006/94‑1 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            >  SRR4302533.1836177/1‑94 (MQ=60)
                                                                   CTCAGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            <  SRR4302533.6337760/94‑1 (MQ=60)
                                                                    TCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAG                           <  SRR4302533.1702885/94‑1 (MQ=60)
                                                                    GCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAG                           <  SRR4302533.5012980/94‑1 (MQ=60)
                                                                       GCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT                        <  SRR4302533.3382516/94‑1 (MQ=60)
                                                                       GCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT                        <  SRR4302533.430406/94‑1 (MQ=60)
                                                                        CGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTA                       <  SRR4302533.5310200/94‑1 (MQ=60)
                                                                        CGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCGGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTA                       <  SRR4302533.4981359/94‑1 (MQ=60)
                                                                        CGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCGGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTA                       <  SRR4302533.5339931/94‑1 (MQ=60)
                                                                           GAATCCGGGGGCCGTTGAGGTTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAA                    <  SRR4302533.261790/94‑1 (MQ=60)
                                                                           GAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAA                    <  SRR4302533.857050/94‑1 (MQ=60)
                                                                           GAACCCGGGGTCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAA                    <  SRR4302533.3861148/94‑1 (MQ=60)
                                                                            AATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAA                   >  SRR4302533.5261351/1‑94 (MQ=60)
                                                                             ATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAG                  >  SRR4302533.2645975/1‑94 (MQ=60)
                                                                             ATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAG                  >  SRR4302533.4455251/1‑94 (MQ=60)
                                                                              TCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGG                 <  SRR4302533.3061983/94‑1 (MQ=60)
                                                                              TCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGG                 <  SRR4302533.1251943/94‑1 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGA                >  SRR4302533.5637111/1‑94 (MQ=60)
                                                                                CGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAT               <  SRR4302533.5877477/94‑1 (MQ=60)
                                                                                CGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAT               <  SRR4302533.2069563/94‑1 (MQ=60)
                                                                                  GTGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302533.2121791/94‑1 (MQ=60)
                                                                                    GGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGA           >  SRR4302533.6078677/1‑94 (MQ=60)
                                                                                     GCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAA          <  SRR4302533.511624/94‑1 (MQ=60)
                                                                                      CCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAT         <  SRR4302533.4099255/94‑1 (MQ=60)
                                                                                       CGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATT        >  SRR4302533.3498084/1‑94 (MQ=60)
                                                                                       CGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATT        <  SRR4302533.192863/94‑1 (MQ=60)
                                                                                        GTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTT       <  SRR4302533.2454167/94‑1 (MQ=60)
                                                                                        GTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTT       >  SRR4302533.1976113/1‑94 (MQ=60)
                                                                                          TGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCGGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTT     <  SRR4302533.2666058/94‑1 (MQ=60)
                                                                                             GGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  <  SRR4302533.5710403/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  >  NC_000913/4134416‑4134602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑