Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,208	A→G	T27T (ACT→ACC)	talC ←	putative transaldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,208	0	A	G	100.0%	804.0 / NA	249	T27T (ACT→ACC)	talC	putative transaldolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (114/135); total (114/135)

Only 100 of 268 total aligned reads displayed.

AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC  >  NC_000913/4137118‑4137297

 aaCAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTAGggttggtt                                                                                        >  2:1906350/1‑93 (MQ=255)

    aGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTc                                                                                     >  2:4970255/1‑93 (MQ=255)

     gAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTcc                                                                                    >  1:553274/1‑92 (MQ=255)

     gAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCa                                                                                    <  2:769942/93‑1 (MQ=255)

     gAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCa                                                                                    <  1:4632879/93‑1 (MQ=255)

     gAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCACCGATAATGCTCGGGTTGGTTGTCa                                                                                    >  1:1001016/1‑93 (MQ=255)

       aTGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAcc                                                                                  <  1:5988607/93‑1 (MQ=255)

        tGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAccc                                                                                 >  2:3801682/1‑93 (MQ=255)

        tGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAccc                                                                                 <  2:1675573/93‑1 (MQ=255)

         gCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAccct                                                                                <  1:289185/93‑2 (MQ=255)

            cTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGc                                                                             >  1:5254422/1‑93 (MQ=255)

            cTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGc                                                                             <  2:5185993/93‑1 (MQ=255)

              catcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCaa                                                                           <  2:5866018/93‑1 (MQ=255)

                tcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACGCTGGCAATg                                                                         >  2:5215154/1‑93 (MQ=255)

                tcaCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATg                                                                         >  2:4120249/1‑93 (MQ=255)

                  aCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATggg                                                                       <  1:4428893/93‑1 (MQ=255)

                  aCCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATggg                                                                       <  1:2926732/93‑1 (MQ=255)

                   ccAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATgggg                                                                      >  2:304051/1‑93 (MQ=255)

                    cAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGa                                                                     >  1:623628/1‑93 (MQ=255)

                    cAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGa                                                                     <  1:2392430/93‑1 (MQ=255)

                    cAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGa                                                                     <  1:1800139/93‑1 (MQ=255)

                    cAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGa                                                                     <  1:3185395/93‑1 (MQ=255)

                     aaTCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGaa                                                                    <  2:4516978/93‑1 (MQ=255)

                     aaTCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGaa                                                                    >  1:1256927/1‑93 (MQ=255)

                       tCGCTTTTTGCAGACGCGGCAGCACTTCCCCTATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTCGCAATGGAGAata                                                                  >  2:2962884/1‑93 (MQ=255)

                       tCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAata                                                                  >  2:2463496/1‑93 (MQ=255)

                         gCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAAtgtg                                                                >  2:4633203/1‑93 (MQ=255)

                          cTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGc                                                               <  2:6072220/93‑1 (MQ=255)

                          cTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGc                                                               <  1:3006976/93‑1 (MQ=255)

                           tttttGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCg                                                              <  2:460429/93‑1 (MQ=255)

                                gCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCa                                                         <  1:1376763/93‑1 (MQ=255)

                                gCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCa                                                         <  1:3147047/93‑1 (MQ=255)

                                  aGACGCGGCAGCATTTCCCATATGGACTCCTTGCTCGCAGCGATAATGCTCAGGATCGTTGTCACCCTGGCAATGGGGTATGTGCGTGCCAGa                                                       >  2:5658217/1‑93 (MQ=255)

                                   gACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGAc                                                      <  2:2010818/93‑1 (MQ=255)

                                   gACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGAc                                                      <  1:2585552/93‑1 (MQ=255)

                                     cgcgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCCCCCTGGCAATGGGGAATATGCGTGCCAGACGt                                                    <  2:4198499/93‑1 (MQ=255)

                                      gcgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGtt                                                   <  1:4832278/93‑1 (MQ=255)

                                       cgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTc                                                  <  1:2961372/93‑1 (MQ=255)

                                       cgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTc                                                  <  2:367794/93‑1 (MQ=255)

                                         gcagcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGa                                                >  2:2655831/1‑93 (MQ=255)

                                          cagcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGAc                                               <  1:3750614/93‑1 (MQ=255)

                                          cagcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGAc                                               <  1:6032262/93‑1 (MQ=255)

                                           agcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACt                                              >  1:6156663/1‑93 (MQ=255)

                                              aCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCt                                           >  2:1513805/1‑93 (MQ=255)

                                                ttCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGc                                         >  2:6257099/1‑93 (MQ=255)

                                                   ccATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGAc                                      >  1:5408661/1‑93 (MQ=255)

                                                   ccATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGACAGACGTTCGACTTCTGCGAc                                      >  1:5851673/1‑93 (MQ=255)

                                                     atatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGt                                    >  1:2740407/1‑93 (MQ=255)

                                                     atatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGCTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGt                                    >  2:5785537/1‑93 (MQ=255)

                                                      tatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGtt                                   <  2:5546523/93‑1 (MQ=255)

                                                      tatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGtt                                   <  2:2188657/93‑1 (MQ=255)

                                                       atGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTa                                  <  2:5375589/93‑1 (MQ=255)

                                                       atGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTa                                  <  1:632761/93‑1 (MQ=255)

                                                        tGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAg                                 <  2:362504/93‑1 (MQ=255)

                                                         ggACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGc                                >  1:4115048/1‑93 (MQ=255)

                                                         ggACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGc                                <  1:3924123/93‑1 (MQ=255)

                                                          gtcTCGTTGCTGGCTGAGATAATGCTCGGGTTGGTTGTCACCATGGCAATGGGGAATATGCGTGCCACACGTTCGACTTCTGCGACGTTAGCg                               <  2:1398272/91‑1 (MQ=255)

                                                          gACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCg                               <  1:4219172/93‑1 (MQ=255)

                                                          gACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCg                               <  1:4051948/93‑1 (MQ=255)

                                                          gACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCg                               <  1:5736442/93‑1 (MQ=255)

                                                           aCTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCgg                              <  1:5621923/93‑1 (MQ=255)

                                                            cTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGTGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgt                             <  1:3526431/93‑1 (MQ=255)

                                                            cTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgt                             <  1:2140334/93‑1 (MQ=255)

                                                            cTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgt                             >  2:4217564/1‑93 (MQ=255)

                                                            cTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgt                             >  1:457415/1‑93 (MQ=255)

                                                             tCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgtg                            <  2:1152722/93‑1 (MQ=255)

                                                             tCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgtg                            >  2:3921114/1‑93 (MQ=255)

                                                              ccTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgtgt                           >  2:2441985/1‑93 (MQ=255)

                                                              ccTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGgtgt                           <  2:3209875/93‑1 (MQ=255)

                                                               cTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTc                          >  2:3747643/1‑93 (MQ=255)

                                                                ttGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTcc                         <  2:4380926/93‑1 (MQ=255)

                                                                  gCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAg                       <  1:5146517/93‑1 (MQ=255)

                                                                  gCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAg                       <  1:4310112/93‑1 (MQ=255)

                                                                   cTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGa                      <  1:1732741/93‑1 (MQ=255)

                                                                   cTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGa                      <  2:2530807/93‑1 (MQ=255)

                                                                   cTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGa                      >  2:6011427/1‑93 (MQ=255)

                                                                    tGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAt                     >  1:2514292/1‑93 (MQ=255)

                                                                       cAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACa                  <  2:3331235/93‑1 (MQ=255)

                                                                       cAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACa                  <  2:4289118/93‑1 (MQ=255)

                                                                       cAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACa                  <  2:3274751/93‑1 (MQ=255)

                                                                        aGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGCCTTCTGCGACGTTAGCGGTGTCCAGATACAg                 >  1:2199937/1‑93 (MQ=255)

                                                                        aGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAg                 <  1:3106991/93‑1 (MQ=255)

                                                                        aGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAg                 <  2:2364389/93‑1 (MQ=255)

                                                                           gATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTc              >  1:4922385/1‑93 (MQ=255)

                                                                           gATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTc              <  2:2582373/93‑1 (MQ=255)

                                                                            aTAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTcc             >  1:165947/1‑93 (MQ=255)

                                                                            aTAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGGGTCCAGATACAGTTcc             >  2:1068848/1‑93 (MQ=255)

                                                                             tAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCa            >  2:87975/1‑93 (MQ=255)

                                                                              aaTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCat           <  1:3469603/93‑1 (MQ=255)

                                                                              aaTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCat           >  2:4821251/1‑93 (MQ=255)

                                                                              aaTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCat           >  2:3994835/1‑93 (MQ=255)

                                                                               aTGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCatg          <  1:167904/93‑1 (MQ=255)

                                                                                tGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCatga         >  2:596568/1‑93 (MQ=255)

                                                                                   tCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGt      >  2:4364002/1‑93 (MQ=255)

                                                                                     gggTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGttt    >  2:617966/1‑93 (MQ=255)

                                                                                      ggttggttGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTc   >  2:5261865/1‑93 (MQ=255)

                                                                                      ggTTGGGTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTc   <  1:5791553/93‑1 (MQ=255)

                                                                                       gttggttgTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTcc  >  1:3856927/1‑93 (MQ=255)

                                                                                       gttggttgTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTcc  <  1:1943442/93‑1 (MQ=255)

                                                                                       gttggttgTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTcc  <  2:2855646/93‑1 (MQ=255)

AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC  >  NC_000913/4137118‑4137297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTT  >  NC_000913/4137115‑4137300

AGCAAACAGAATGCCCTTATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTG                                                                                              >  SRR4302533.2019581/1‑94 (MQ=60)

   AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTT                                                                                           <  SRR4302533.1620717/94‑1 (MQ=60)

    AACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTG                                                                                          <  SRR4302533.4659660/94‑1 (MQ=60)

     ACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGT                                                                                         >  SRR4302533.2915588/1‑94 (MQ=60)

      CAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTC                                                                                        >  SRR4302533.1475023/1‑94 (MQ=60)

       AGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCC                                                                                       >  SRR4302533.556554/1‑94 (MQ=60)

       AGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCA                                                                                       >  SRR4302533.6235358/1‑94 (MQ=60)

       AGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCACCGATAATGCTCGGGTTGGTTGTCA                                                                                       >  SRR4302533.1006860/1‑94 (MQ=60)

        GAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAC                                                                                      >  SRR4302533.4258507/1‑94 (MQ=60)

        GAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCAC                                                                                      <  SRR4302533.4660955/94‑1 (MQ=60)

          ATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCC                                                                                    <  SRR4302533.6024876/94‑1 (MQ=60)

            GCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTG                                                                                  <  SRR4302533.1759188/94‑1 (MQ=60)

            GCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTG                                                                                  <  SRR4302533.5342072/94‑1 (MQ=60)

            GCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTG                                                                                  <  SRR4302533.290949/94‑1 (MQ=60)

             CCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGG                                                                                 <  SRR4302533.2793719/94‑1 (MQ=60)

              CCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGC                                                                                >  SRR4302533.5285988/1‑94 (MQ=60)

              CCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGC                                                                                <  SRR4302533.1951897/94‑1 (MQ=60)

               CTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCA                                                                               <  SRR4302533.5888173/94‑1 (MQ=60)

                  ATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATG                                                                            >  SRR4302533.3851071/1‑94 (MQ=60)

                  ATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATG                                                                            >  SRR4302533.3054983/1‑94 (MQ=60)

                     ACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGG                                                                         >  SRR4302533.3792191/1‑94 (MQ=60)

                     ACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGG                                                                         <  SRR4302533.2944639/94‑1 (MQ=60)

                     ACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGG                                                                         <  SRR4302533.4455667/94‑1 (MQ=60)

                      CCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGA                                                                        <  SRR4302533.5701873/94‑1 (MQ=60)

                      CCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGA                                                                        >  SRR4302533.627290/1‑94 (MQ=60)

                       CAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAA                                                                       <  SRR4302533.3204986/94‑1 (MQ=60)

                       CAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAA                                                                       <  SRR4302533.1811092/94‑1 (MQ=60)

                       CAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAA                                                                       >  SRR4302533.1264441/1‑94 (MQ=60)

                       CAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAA                                                                       <  SRR4302533.2407198/94‑1 (MQ=60)

                          TCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATAT                                                                    <  SRR4302533.2497659/94‑1 (MQ=60)

                          TCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATAT                                                                    <  SRR4302533.1732737/94‑1 (MQ=60)

                           CGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATG                                                                   >  SRR4302533.317333/1‑94 (MQ=60)

                            GCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGC                                                                  <  SRR4302533.4354840/94‑1 (MQ=60)

                            GCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGC                                                                  <  SRR4302533.4233462/94‑1 (MQ=60)

                            GCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGC                                                                  >  SRR4302533.4221856/1‑94 (MQ=60)

                             CTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCG                                                                 <  SRR4302533.2193794/94‑1 (MQ=60)

                             CTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCG                                                                 <  SRR4302533.3536099/94‑1 (MQ=60)

                             CTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCG                                                                 <  SRR4302533.3025368/94‑1 (MQ=60)

                                   GCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAG                                                           <  SRR4302533.3166397/94‑1 (MQ=60)

                                   GCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAG                                                           <  SRR4302533.1385046/94‑1 (MQ=60)

                                   GCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAG                                                           <  SRR4302533.4859019/94‑1 (MQ=60)

                                    CAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGA                                                          >  SRR4302533.5138063/1‑94 (MQ=60)

                                     AGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACTCTGGCAATGGGGAATATGCGTGCCAGAC                                                         >  SRR4302533.3192778/1‑94 (MQ=60)

                                     AGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGAC                                                         >  SRR4302533.842635/1‑94 (MQ=60)

                                      GACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACG                                                        <  SRR4302533.2601449/94‑1 (MQ=60)

                                        CGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTT                                                      <  SRR4302533.2611204/94‑1 (MQ=60)

                                         GCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTC                                                     <  SRR4302533.4861567/94‑1 (MQ=60)

                                         GCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTC                                                     <  SRR4302533.3598453/94‑1 (MQ=60)

                                         GCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTC                                                     <  SRR4302533.585950/94‑1 (MQ=60)

                                         GCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTC                                                     >  SRR4302533.560922/1‑94 (MQ=60)

                                         GCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTC                                                     <  SRR4302533.3113172/94‑1 (MQ=60)

                                          CGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCG                                                    <  SRR4302533.410962/94‑1 (MQ=60)

                                          CGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCG                                                    <  SRR4302533.2979475/94‑1 (MQ=60)

                                           GGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGA                                                   <  SRR4302533.2764490/94‑1 (MQ=60)

                                             CAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACT                                                 >  SRR4302533.6193701/1‑94 (MQ=60)

                                             CAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACT                                                 <  SRR4302533.6068820/94‑1 (MQ=60)

                                             CAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACT                                                 <  SRR4302533.3773610/94‑1 (MQ=60)

                                             CAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACT                                                 >  SRR4302533.1363788/1‑94 (MQ=60)

                                              AGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTT                                                <  SRR4302533.4940077/94‑1 (MQ=60)

                                                CACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCT                                              <  SRR4302533.525700/94‑1 (MQ=60)

                                                 ACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTG                                             >  SRR4302533.4339477/1‑94 (MQ=60)

                                                  CTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGC                                            >  SRR4302533.2994665/1‑94 (MQ=60)

                                                  CTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGC                                            >  SRR4302533.1695589/1‑94 (MQ=60)

                                                     CCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGAC                                         <  SRR4302533.2160051/94‑1 (MQ=60)

                                                     CCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGAC                                         >  SRR4302533.5441171/1‑94 (MQ=60)

                                                     CCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGACAGACGTTCGACTTCTGCGAC                                         >  SRR4302533.5887051/1‑94 (MQ=60)

                                                      CCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACG                                        >  SRR4302533.4092459/1‑94 (MQ=60)

                                                       CATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGT                                       >  SRR4302533.2757210/1‑94 (MQ=60)

                                                         TATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTA                                     <  SRR4302533.6003299/94‑1 (MQ=60)

                                                         TATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTA                                     <  SRR4302533.123429/94‑1 (MQ=60)

                                                          ATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAG                                    <  SRR4302533.636484/94‑1 (MQ=60)

                                                          ATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAG                                    <  SRR4302533.3255868/94‑1 (MQ=60)

                                                          ATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAG                                    >  SRR4302533.6207279/1‑94 (MQ=60)

                                                           TGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGC                                   <  SRR4302533.2560014/94‑1 (MQ=60)

                                                           TGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGC                                   <  SRR4302533.4493461/94‑1 (MQ=60)

                                                           TGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGC                                   >  SRR4302533.4140053/1‑94 (MQ=60)

                                                            GGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCG                                  <  SRR4302533.1689035/94‑1 (MQ=60)

                                                            GGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCG                                  <  SRR4302533.3948083/94‑1 (MQ=60)

                                                            GGACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGCTCGACTTCTGCGACGTTAGCG                                  >  SRR4302533.1312323/1‑94 (MQ=60)

                                                             GACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGG                                 <  SRR4302533.4244689/94‑1 (MQ=60)

                                                             GACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGG                                 <  SRR4302533.5771065/94‑1 (MQ=60)

                                                             GACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGG                                 >  SRR4302533.564071/1‑94 (MQ=60)

                                                             GACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGG                                 <  SRR4302533.5178953/94‑1 (MQ=60)

                                                             GACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGG                                 <  SRR4302533.4076625/94‑1 (MQ=60)

                                                              ACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGT                                <  SRR4302533.5655905/94‑1 (MQ=60)

                                                              ACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGT                                >  SRR4302533.460151/1‑94 (MQ=60)

                                                              ACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGT                                <  SRR4302533.6042454/94‑1 (MQ=60)

                                                              ACTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGT                                >  SRR4302533.3894863/1‑94 (MQ=60)

                                                               CTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGTGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTG                               <  SRR4302533.3548162/94‑1 (MQ=60)

                                                               CTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTG                               <  SRR4302533.4713918/94‑1 (MQ=60)

                                                               CTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTG                               >  SRR4302533.2923269/1‑94 (MQ=60)

                                                               CTCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTG                               <  SRR4302533.2153528/94‑1 (MQ=60)

                                                                TCCTTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGT                              >  SRR4302533.2970437/1‑94 (MQ=60)

                                                                   TTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCA                           <  SRR4302533.2022343/94‑1 (MQ=60)

                                                                   TTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCA                           <  SRR4302533.4932198/94‑1 (MQ=60)

                                                                   TTGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCA                           <  SRR4302533.5869797/94‑1 (MQ=60)

                                                                    TGCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTCGCGGTGTCCAG                          >  SRR4302533.3939912/1‑94 (MQ=60)

                                                                     GCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGA                         <  SRR4302533.4336057/94‑1 (MQ=60)

                                                                     GCTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGA                         <  SRR4302533.5177619/94‑1 (MQ=60)

                                                                      CTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAT                        >  SRR4302533.5784283/1‑94 (MQ=60)

                                                                      CTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAT                        <  SRR4302533.1743245/94‑1 (MQ=60)

                                                                      CTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAT                        <  SRR4302533.5401474/94‑1 (MQ=60)

                                                                      CTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAT                        >  SRR4302533.2529784/1‑94 (MQ=60)

                                                                      CTGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGAT                        >  SRR4302533.329681/1‑94 (MQ=60)

                                                                       TGGCAGCGATAATGCTCGGGTTGGTTGTCACCCTAGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAACGGTGTCCAGATA                       >  SRR4302533.1447847/1‑94 (MQ=60)

                                                                          CAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGCCTTCTGCGACGTTAGCGGTGTCCAGATACAG                    >  SRR4302533.2213490/1‑94 (MQ=60)

                                                                          CAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAG                    <  SRR4302533.5739455/94‑1 (MQ=60)

                                                                          CAGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAG                    <  SRR4302533.6171324/94‑1 (MQ=60)

                                                                           AGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGT                   <  SRR4302533.3126087/94‑1 (MQ=60)

                                                                           AGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGT                   <  SRR4302533.4579751/94‑1 (MQ=60)

                                                                           AGCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGT                   <  SRR4302533.4024622/94‑1 (MQ=60)

                                                                            GCGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTT                  >  SRR4302533.3649627/1‑94 (MQ=60)

                                                                             CGATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTC                 >  SRR4302533.4952203/1‑94 (MQ=60)

                                                                              GATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCC                >  SRR4302533.2986404/1‑94 (MQ=60)

                                                                              GATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCC                >  SRR4302533.2574279/1‑94 (MQ=60)

                                                                              GATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCC                >  SRR4302533.167000/1‑94 (MQ=60)

                                                                              GAGAATGCTCGGGTTGGTTGTCGCCCTGGCAATGGGGAATATGCGTGCCATACGTTCGTCGTCTGCGACGTTAGCGGTGTCCAGATACAGTTCC                <  SRR4302533.3128739/94‑1 (MQ=60)

                                                                               ATAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGTTACAGTTCCA               <  SRR4302533.6338726/94‑1 (MQ=60)

                                                                                TAATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCAT              <  SRR4302533.961505/94‑1 (MQ=60)

                                                                                 AATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATG             <  SRR4302533.3491020/94‑1 (MQ=60)

                                                                                  ATGCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGA            <  SRR4302533.168965/94‑1 (MQ=60)

                                                                                    GCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATG          <  SRR4302533.179100/94‑1 (MQ=60)

                                                                                    GCTCGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATG          <  SRR4302533.6169882/94‑1 (MQ=60)

                                                                                       CGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTT       <  SRR4302533.2048691/94‑1 (MQ=60)

                                                                                       CGGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTT       <  SRR4302533.2371214/94‑1 (MQ=60)

                                                                                        GGGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTC      >  SRR4302533.2441582/1‑94 (MQ=60)

                                                                                         GGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC     >  SRR4302533.5548035/1‑94 (MQ=60)

                                                                                         GGTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC     >  SRR4302533.3880475/1‑94 (MQ=60)

                                                                                         GGTTGGGTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC     <  SRR4302533.5826542/94‑1 (MQ=60)

                                                                                          GTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCT    >  SRR4302533.1811788/1‑94 (MQ=60)

                                                                                          GTTGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCT    <  SRR4302533.1955297/94‑1 (MQ=60)

                                                                                            TGGTTGTCACCCTGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTT  >  SRR4302533.4552793/1‑94 (MQ=60)

AGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTT  >  NC_000913/4137115‑4137300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`