Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,149,444	A→G	Y412H (TAC→CAC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,149,444	0	A	G	99.6%	845.4 / NA	269	Y412H (TAC→CAC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (134/133); minor base T (0/1); total (134/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

  aGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCgg                                                                                       >  1:5905250/1‑93 (MQ=255)

   gAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCggg                                                                                      >  1:156499/1‑93 (MQ=255)

   gAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCggg                                                                                      >  2:1277965/1‑93 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    >  1:4462698/1‑93 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  1:4135971/93‑1 (MQ=255)

      gCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCa                                                                                   <  2:740776/93‑1 (MQ=255)

        ggACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGc                                                                                 >  2:4568059/1‑93 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                >  1:3989945/1‑93 (MQ=255)

           cGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCg                                                                              >  2:560740/1‑93 (MQ=255)

            gTTTGGAGTTAAGTTCGCGGATCAGGAACGCCTGTTTGTCAGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGc                                                                             >  2:2298462/1‑93 (MQ=255)

             tttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGcc                                                                            <  1:931026/93‑1 (MQ=255)

             tttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGcc                                                                            >  2:4482965/1‑93 (MQ=255)

             tttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGcc                                                                            >  1:4616005/1‑93 (MQ=255)

               tGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCa                                                                          >  1:3316077/1‑93 (MQ=255)

               tGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCa                                                                          <  1:1900718/93‑1 (MQ=255)

                ggAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCCGCTCGCCCAg                                                                         >  2:3166363/1‑93 (MQ=255)

                ggAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGAGGCGGGCCAGGTCGCCCAg                                                                         >  2:1938727/1‑93 (MQ=255)

                 gAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgc                                                                        <  2:5467279/93‑1 (MQ=255)

                 gAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgc                                                                        >  2:2651843/1‑93 (MQ=255)

                   gttcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgcgc                                                                      >  1:3144261/1‑93 (MQ=255)

                     tcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCCGCGCtt                                                                    >  2:2192165/1‑93 (MQ=255)

                     tcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCtt                                                                    <  1:529366/93‑1 (MQ=255)

                       agttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCg                                                                  <  1:4839513/93‑1 (MQ=255)

                         ttcGCGTATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGt                                                                >  1:3016126/1‑93 (MQ=255)

                         ttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGt                                                                <  1:3494133/93‑1 (MQ=255)

                         ttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGt                                                                <  2:3823417/93‑1 (MQ=255)

                          tcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTa                                                               <  2:1359331/93‑1 (MQ=255)

                            gcgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATg                                                             >  1:2843288/1‑93 (MQ=255)

                            gcgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATg                                                             <  1:2072381/93‑1 (MQ=255)

                             gggATCAGGAACGCCTGTTTGTCGGCCTCTGAACAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:4699925/92‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  1:1046647/93‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:1881382/93‑1 (MQ=255)

                              ggATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAc                                                           <  1:4498873/93‑1 (MQ=255)

                              ggATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAc                                                           <  2:6053972/93‑1 (MQ=255)

                               gATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcg                                                          <  2:2900334/93‑1 (MQ=255)

                               gATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcg                                                          >  1:2445428/1‑93 (MQ=255)

                                 tCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgcg                                                        >  1:3080806/1‑93 (MQ=255)

                                  cAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGt                                                       <  2:5170030/93‑1 (MQ=255)

                                     gAATGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGct                                                    <  2:3765682/93‑1 (MQ=255)

                                     gAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGct                                                    <  1:6202143/93‑1 (MQ=255)

                                      aaCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   >  1:3216881/1‑93 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTTGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               >  2:1446864/1‑93 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               >  2:5523309/1‑93 (MQ=255)

                                           cTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  2:596232/1‑93 (MQ=255)

                                           cTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  2:1139399/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCTCTTCGGTATGACGCCTGCTTTTCTGa                                             >  2:486632/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             >  1:558762/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             >  1:5544750/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             >  1:745653/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             <  2:3409455/93‑1 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            >  1:1123668/1‑93 (MQ=255)

                                               ttGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACgg                                          <  1:1161418/93‑1 (MQ=255)

                                               ttGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGAGCTTCGGTATGACGCGTGCTCTCCTGCCgg                                          >  1:4401738/1‑93 (MQ=255)

                                                tGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGa                                         <  2:4381387/93‑1 (MQ=255)

                                                tGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGa                                         <  1:3027927/93‑1 (MQ=255)

                                                tGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGa                                         >  2:785743/1‑93 (MQ=255)

                                                 gTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGat                                        <  1:4913427/93‑1 (MQ=255)

                                                 gTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGat                                        >  1:3831206/1‑93 (MQ=255)

                                                  tCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGata                                       >  2:5749066/1‑93 (MQ=255)

                                                    ggCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGACATc                                     >  1:3699292/1‑93 (MQ=255)

                                                    ggCCTCTGACAAGCTTTCGTAGTCGCCGATACCGAGGT‑GCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    <  2:941845/93‑1 (MQ=255)

                                                     gCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    >  1:5090905/1‑93 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  <  1:631360/93‑1 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  2:5496644/1‑93 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  2:2852044/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  2:4186660/93‑1 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 >  1:3856062/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:2441473/93‑1 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:2211687/93‑1 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  2:2061707/93‑1 (MQ=255)

                                                         ctGACCAGCTTTCGTAGTCGCCGCTACCGAGGTGGCGGGTCAGCTCACCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAc                                >  2:5071651/1‑93 (MQ=255)

                                                         ctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAc                                <  1:4772862/93‑1 (MQ=255)

                                                         ctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAc                                <  1:6066581/93‑1 (MQ=255)

                                                          tGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACg                               <  2:5557558/93‑1 (MQ=255)

                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             <  1:5829179/93‑1 (MQ=255)

                                                             ccAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAc                            <  2:3037984/93‑1 (MQ=255)

                                                             ccAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGCATGACGCGTGCTCTCCTGACGGATATCAATACGGAc                            >  1:1952117/1‑93 (MQ=255)

                                                              cAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAcc                           >  2:5879406/1‑93 (MQ=255)

                                                                 cTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGc                        <  1:1421380/93‑1 (MQ=255)

                                                                  tttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCg                       <  2:5392370/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      >  1:1986142/1‑93 (MQ=255)

                                                                    tCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGt                     >  2:254232/1‑93 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  2:4314189/93‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  1:3111649/93‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  2:1576142/93‑1 (MQ=255)

                                                                       tAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACg                  >  2:1004873/1‑93 (MQ=255)

                                                                            gCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaa             <  2:3728635/93‑1 (MQ=255)

                                                                             ccGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaaa            >  2:335422/1‑93 (MQ=255)

                                                                             ccGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaaa            <  1:2905268/93‑1 (MQ=255)

                                                                             ccGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaaa            >  2:1855107/1‑93 (MQ=255)

                                                                               gATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACa          <  1:2685453/93‑1 (MQ=255)

                                                                                attccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         <  1:1790065/90‑1 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        >  2:3987781/1‑93 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  2:3639695/93‑1 (MQ=255)

                                                                                    cGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCa     <  2:2796901/93‑1 (MQ=255)

                                                                                     gAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAc    <  1:1673320/93‑1 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   >  1:804202/1‑93 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   >  1:5737723/1‑93 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   >  2:2351336/1‑93 (MQ=255)

                                                                                       ggTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcgc  >  2:237881/1‑93 (MQ=255)

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  >  NC_000913/4149351‑4149537

GCGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTG                                                                                               <  SRR4302533.462545/94‑1 (MQ=60)

 CGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGG                                                                                              <  SRR4302533.3250167/94‑1 (MQ=60)

 CGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGG                                                                                              <  SRR4302533.2374534/94‑1 (MQ=60)

  GGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGC                                                                                             >  SRR4302533.3254547/1‑94 (MQ=60)

  GGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGC                                                                                             >  SRR4302533.1863870/1‑94 (MQ=60)

    CAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGG                                                                                           >  SRR4302533.5940913/1‑94 (MQ=60)

     AGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGG                                                                                          >  SRR4302533.6238699/1‑94 (MQ=60)

     AGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGG                                                                                          >  SRR4302533.3309836/1‑94 (MQ=60)

     AGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGG                                                                                          >  SRR4302533.157496/1‑94 (MQ=60)

      GAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGT                                                                                         >  SRR4302533.3635598/1‑94 (MQ=60)

       AAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTC                                                                                        >  SRR4302533.4489691/1‑94 (MQ=60)

        AGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCA                                                                                       <  SRR4302533.4161073/94‑1 (MQ=60)

          CGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGC                                                                                     >  SRR4302533.657814/1‑94 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    >  SRR4302533.4729476/1‑94 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    >  SRR4302533.4014248/1‑94 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    >  SRR4302533.33523/1‑94 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    <  SRR4302533.2459931/94‑1 (MQ=60)

             GCGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCG                                                                                  <  SRR4302533.876684/94‑1 (MQ=60)

              CGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCCCGC                                                                                 >  SRR4302533.1414890/1‑94 (MQ=60)

               GTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCC                                                                                >  SRR4302533.2078711/1‑94 (MQ=60)

               GTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCC                                                                                >  SRR4302533.4643958/1‑94 (MQ=60)

               GTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCC                                                                                >  SRR4302533.683246/1‑94 (MQ=60)

                TTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCC                                                                               <  SRR4302533.936490/94‑1 (MQ=60)

                 TTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCA                                                                              >  SRR4302533.3336554/1‑94 (MQ=60)

                  TGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAG                                                                             <  SRR4302533.5715911/94‑1 (MQ=60)

                  TGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAG                                                                             <  SRR4302533.1912325/94‑1 (MQ=60)

                    GAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCG                                                                           <  SRR4302533.1536839/94‑1 (MQ=60)

                     AGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGC                                                                          >  SRR4302533.3163592/1‑94 (MQ=60)

                     AGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGC                                                                          >  SRR4302533.5735531/1‑94 (MQ=60)

                      GTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCGGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCT                                                                         >  SRR4302533.287980/1‑94 (MQ=60)

                       TTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTT                                                                        >  SRR4302533.284151/1‑94 (MQ=60)

                       TTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTT                                                                        <  SRR4302533.2012734/94‑1 (MQ=60)

                        TCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTC                                                                       <  SRR4302533.4934767/94‑1 (MQ=60)

                        TCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTC                                                                       <  SRR4302533.532513/94‑1 (MQ=60)

                         CAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCG                                                                      <  SRR4302533.5859382/94‑1 (MQ=60)

                          AGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGG                                                                     <  SRR4302533.1557108/94‑1 (MQ=60)

                          AGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGG                                                                     <  SRR4302533.2542073/94‑1 (MQ=60)

                          AGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGG                                                                     <  SRR4302533.4868846/94‑1 (MQ=60)

                           GTTCGCGTATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGT                                                                    >  SRR4302533.3034574/1‑94 (MQ=60)

                            TTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTA                                                                   <  SRR4302533.3515686/94‑1 (MQ=60)

                            TTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTA                                                                   <  SRR4302533.4832/94‑1 (MQ=60)

                             TCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTAT                                                                  >  SRR4302533.5172478/1‑94 (MQ=60)

                              CGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATG                                                                 <  SRR4302533.3045455/94‑1 (MQ=60)

                              CGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATG                                                                 >  SRR4302533.2860785/1‑94 (MQ=60)

                               GCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGA                                                                <  SRR4302533.2085146/94‑1 (MQ=60)

                                CGGATCAGTAACGCCTGTTTTTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAC                                                               <  SRR4302533.1783652/94‑1 (MQ=60)

                                CGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAC                                                               <  SRR4302533.1052796/94‑1 (MQ=60)

                                CGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAC                                                               >  SRR4302533.2460057/1‑94 (MQ=60)

                                 GGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACG                                                              <  SRR4302533.4526117/94‑1 (MQ=60)

                                 GGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACG                                                              <  SRR4302533.765901/94‑1 (MQ=60)

                                 GGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACG                                                              >  SRR4302533.2460524/1‑94 (MQ=60)

                                  GATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGC                                                             >  SRR4302533.4997663/1‑94 (MQ=60)

                                   ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                            <  SRR4302533.1164452/94‑1 (MQ=60)

                                   ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                            >  SRR4302533.1166988/1‑94 (MQ=60)

                                   ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                            >  SRR4302533.5281379/1‑94 (MQ=60)

                                   ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                            >  SRR4302533.3099707/1‑94 (MQ=60)

                                       GGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCT                                                        >  SRR4302533.10024/1‑94 (MQ=60)

                                        GAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTC                                                       <  SRR4302533.4569506/94‑1 (MQ=60)

                                        GAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTC                                                       <  SRR4302533.1929736/94‑1 (MQ=60)

                                        GAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTC                                                       <  SRR4302533.6239341/94‑1 (MQ=60)

                                        GAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTC                                                       >  SRR4302533.3236716/1‑94 (MQ=60)

                                        GAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTC                                                       >  SRR4302533.2935101/1‑94 (MQ=60)

                                         AACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCT                                                      <  SRR4302533.989623/94‑1 (MQ=60)

                                           CGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCC                                                    <  SRR4302533.450564/94‑1 (MQ=60)

                                            GCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCT                                                   <  SRR4302533.3106587/94‑1 (MQ=60)

                                             CGTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  <  SRR4302533.9250/94‑1 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  >  SRR4302533.4244371/1‑94 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  >  SRR4302533.4374594/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGC                                                 >  SRR4302533.1633720/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302533.750042/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302533.3151905/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302533.4898871/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302533.5892749/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302533.5578240/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302533.562083/1‑94 (MQ=60)

                                               TGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCCGCGCTTCGGTTTGACGggggttttcctgcg                                                >  SRR4302533.1181452/1‑80 (MQ=60)

                                               TGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAC                                                <  SRR4302533.1921417/94‑1 (MQ=60)

                                               TGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAC                                                >  SRR4302533.1130336/1‑94 (MQ=60)

                                               TGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAC                                                >  SRR4302533.4661212/1‑94 (MQ=60)

                                                GTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACG                                               <  SRR4302533.3872041/94‑1 (MQ=60)

                                                 TTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGG                                              >  SRR4302533.4132433/1‑94 (MQ=60)

                                                 TTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGAGCTTCGGTATGACGCGTGCTCTCCTGCCGG                                              >  SRR4302533.4428317/1‑94 (MQ=60)

                                                  TTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGA                                             <  SRR4302533.1989338/94‑1 (MQ=60)

                                                  TTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGA                                             <  SRR4302533.1168388/94‑1 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                            >  SRR4302533.3854627/1‑94 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                            <  SRR4302533.2872863/94‑1 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                            <  SRR4302533.3823021/94‑1 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                            <  SRR4302533.3046458/94‑1 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGAGTGCTCTCCTGACGGAT                                            <  SRR4302533.2356266/94‑1 (MQ=60)

                                                    GTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATA                                           <  SRR4302533.4943188/94‑1 (MQ=60)

                                                      CGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATC                                         <  SRR4302533.5843108/94‑1 (MQ=60)

                                                      CGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGACATC                                         >  SRR4302533.3722011/1‑94 (MQ=60)

                                                       GGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCA                                        >  SRR4302533.5121663/1‑94 (MQ=60)

                                                        GCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAA                                       >  SRR4302533.3571130/1‑94 (MQ=60)

                                                        GCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAA                                       >  SRR4302533.5578385/1‑94 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      <  SRR4302533.6198057/94‑1 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      >  SRR4302533.1775013/1‑94 (MQ=60)

                                                          CTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATA                                     <  SRR4302533.635075/94‑1 (MQ=60)

                                                          CTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATA                                     >  SRR4302533.3879607/1‑94 (MQ=60)

                                                          CTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATA                                     >  SRR4302533.3882167/1‑94 (MQ=60)

                                                           TCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAC                                    <  SRR4302533.2456547/94‑1 (MQ=60)

                                                           TCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAC                                    <  SRR4302533.2225327/94‑1 (MQ=60)

                                                            CTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACG                                   <  SRR4302533.4801785/94‑1 (MQ=60)

                                                            CTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACG                                   >  SRR4302533.3212769/1‑94 (MQ=60)

                                                            CTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACG                                   <  SRR4302533.4131236/94‑1 (MQ=60)

                                                            CTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACG                                   <  SRR4302533.6103296/94‑1 (MQ=60)

                                                             TGTCCAGCTTTCGTAGTCGCCGATACCGAGGTTGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGG                                  <  SRR4302533.1903669/94‑1 (MQ=60)

                                                             TGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGG                                  <  SRR4302533.3099255/94‑1 (MQ=60)

                                                              GACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGA                                 >  SRR4302533.382749/1‑94 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                <  SRR4302533.5864403/94‑1 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                <  SRR4302533.6335058/94‑1 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGCATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                >  SRR4302533.1964015/1‑94 (MQ=60)

                                                                 CAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCA                              <  SRR4302533.2997516/94‑1 (MQ=60)

                                                                  AGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAG                             >  SRR4302533.2216261/1‑94 (MQ=60)

                                                                  AGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAG                             >  SRR4302533.5486661/1‑94 (MQ=60)

                                                                    CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCG                           <  SRR4302533.1429976/94‑1 (MQ=60)

                                                                    CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCG                           >  SRR4302533.4455410/1‑94 (MQ=60)

                                                                    CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCG                           >  SRR4302533.5936788/1‑94 (MQ=60)

                                                                     TTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGG                          >  SRR4302533.1998294/1‑94 (MQ=60)

                                                                       TCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTA                        <  SRR4302533.2191224/94‑1 (MQ=60)

                                                                       TCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTA                        <  SRR4302533.4514959/94‑1 (MQ=60)

                                                                        CGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAC                       <  SRR4302533.3130781/94‑1 (MQ=60)

                                                                         GTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACG                      <  SRR4302533.6255780/94‑1 (MQ=60)

                                                                          TAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGC                     >  SRR4302533.5986657/1‑94 (MQ=60)

                                                                           AGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCC                    <  SRR4302533.3151275/94‑1 (MQ=60)

                                                                           AGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCC                    >  SRR4302533.2860746/1‑94 (MQ=60)

                                                                              CGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAA                 >  SRR4302533.1053918/1‑94 (MQ=60)

                                                                                CCGCTACCGAGGTGGCGGTTCAGCTCGCCCAGCGCTTCGGTAGGACGCGTGCGCTCATGACGCATATTAATACGGACCAGCGGTACGCCGAAAC               <  SRR4302533.4544023/94‑1 (MQ=60)

                                                                                CCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAC               <  SRR4302533.2923111/94‑1 (MQ=60)

                                                                                CCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAC               >  SRR4302533.307849/1‑94 (MQ=60)

                                                                                  GATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAT             <  SRR4302533.2701908/94‑1 (MQ=60)

                                                                                   ATTCCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATT            <  SRR4302533.1800952/94‑1 (MQ=60)

                                                                                   ATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATT            >  SRR4302533.4960048/1‑94 (MQ=60)

                                                                                     ACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTC          <  SRR4302533.546343/94‑1 (MQ=60)

                                                                                     ACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTC          <  SRR4302533.4612907/94‑1 (MQ=60)

                                                                                      CCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCA         <  SRR4302533.389789/94‑1 (MQ=60)

                                                                                      CCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCA         >  SRR4302533.2339468/1‑94 (MQ=60)

                                                                                       CGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCCCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAC        >  SRR4302533.339478/1‑94 (MQ=60)

                                                                                        GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG       <  SRR4302533.1683433/94‑1 (MQ=60)

                                                                                        GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG       >  SRR4302533.5772354/1‑94 (MQ=60)

                                                                                        GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG       >  SRR4302533.808926/1‑94 (MQ=60)

                                                                                          GGTGGCGTGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCG     <  SRR4302533.3128170/94‑1 (MQ=60)

                                                                                          GGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCG     >  SRR4302533.2774183/1‑94 (MQ=60)

                                                                                           GTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGG    <  SRR4302533.4835553/94‑1 (MQ=60)

                                                                                           GTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGG    <  SRR4302533.6189922/94‑1 (MQ=60)

                                                                                           GTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGG    <  SRR4302533.6138997/94‑1 (MQ=60)

                                                                                            TGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGC   <  SRR4302533.3392292/94‑1 (MQ=60)

                                                                                             GGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  <  SRR4302533.4342148/94‑1 (MQ=60)

                                                                                             GGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  <  SRR4302533.1714922/94‑1 (MQ=60)

GCGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  >  NC_000913/4149351‑4149537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`