Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,158,977	T→C	V111A (GTC→GCC)	yijC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,158,977	0	T	C	99.6%	820.5 / NA	259	V111A (GTC→GCC)	yijC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (123/135); minor base G (1/0); total (124/135)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

TGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTG  >  NC_000913/4158887‑4159066

tGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGcct                                                                                         >  2:2520727/1‑93 (MQ=255)

  gTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTcc                                                                                       >  1:371499/1‑93 (MQ=255)

  gTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTcc                                                                                       >  2:421035/1‑93 (MQ=255)

   ttGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCca                                                                                      <  2:137645/93‑1 (MQ=255)

   ttGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCca                                                                                      <  1:319043/93‑1 (MQ=255)

    tgatgaGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCcac                                                                                     <  1:5621429/93‑1 (MQ=255)

      atgaGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACAt                                                                                   <  2:5845993/93‑1 (MQ=255)

       tgaGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACAtt                                                                                  >  1:5945567/1‑93 (MQ=255)

       tgaGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACAtt                                                                                  <  2:5933434/93‑1 (MQ=255)

       tgaGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACAtt                                                                                  <  1:2830784/93‑1 (MQ=255)

       tgaGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCGCACCTCGGCCTCCATAtt                                                                                  >  2:2175043/1‑93 (MQ=255)

        gagaGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACAttt                                                                                 <  2:5362066/93‑1 (MQ=255)

        gagaGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACAttt                                                                                 >  1:3032507/1‑93 (MQ=255)

         agagCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTa                                                                                <  1:5875860/93‑1 (MQ=255)

         agagCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTa                                                                                <  1:6128865/93‑1 (MQ=255)

         agagCAGTTGAATGCAACGCCAACTCATGCGCCAGGCGCGTCAGCTTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTa                                                                                <  2:564845/93‑1 (MQ=255)

            gCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATgg                                                                             <  1:1886903/93‑1 (MQ=255)

            gCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATgg                                                                             >  1:3828117/1‑93 (MQ=255)

             cGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGa                                                                            <  2:2374663/93‑1 (MQ=255)

             cGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGa                                                                            <  2:1691657/93‑1 (MQ=255)

             aggTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGa                                                                            <  1:3798914/92‑1 (MQ=255)

               gTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGt                                                                          >  1:4307078/1‑93 (MQ=255)

               gTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGt                                                                          >  2:4051856/1‑93 (MQ=255)

               gTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGt                                                                          >  1:3646494/1‑93 (MQ=255)

                tttAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGtt                                                                         >  1:1568638/1‑93 (MQ=255)

                tttAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGtt                                                                         >  2:6060413/1‑93 (MQ=255)

                  tAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCa                                                                       <  2:3080334/93‑1 (MQ=255)

                  tAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCa                                                                       <  1:6239525/93‑1 (MQ=255)

                  tAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCa                                                                       >  2:294270/1‑93 (MQ=255)

                    aTGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATc                                                                     <  2:3819788/93‑1 (MQ=255)

                    aTGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATc                                                                     <  2:4836718/93‑1 (MQ=255)

                    aTGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATc                                                                     >  1:2133568/1‑93 (MQ=255)

                     tGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCTGCACCTCGGCCTCCACATTTATGGAGTTCATCg                                                                    <  1:687565/93‑1 (MQ=255)

                      gCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCgg                                                                   >  1:2626521/1‑93 (MQ=255)

                       cTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGt                                                                  <  1:4392971/93‑1 (MQ=255)

                         aCGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGtaa                                                                <  2:97527/93‑1 (MQ=255)

                         aCGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGtaa                                                                >  1:2290222/1‑93 (MQ=255)

                         aCGCCAACTCAGGCGCCAGGCGCGTCAGCGTAGCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGtaa                                                                <  2:2854894/93‑1 (MQ=255)

                            ccAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGtaataa                                                             <  1:2319422/93‑1 (MQ=255)

                            ccAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGtaataa                                                             >  2:5184042/1‑93 (MQ=255)

                                cTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCt                                                         <  1:2849237/93‑1 (MQ=255)

                                cTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCt                                                         >  1:1534401/1‑93 (MQ=255)

                                 tCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTa                                                        <  1:2936661/93‑1 (MQ=255)

                                  cATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTaa                                                       <  2:1792649/93‑1 (MQ=255)

                                  cATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTaa                                                       <  2:2803676/93‑1 (MQ=255)

                                  cATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTaa                                                       >  1:5805023/1‑93 (MQ=255)

                                  cATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTaa                                                       <  1:2867799/93‑1 (MQ=255)

                                   aggcgcGAGGCGCGTCATCGTATCGCGAAAGGCGGGAGGGTGATCCGCACCGCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAc                                                      <  2:2636550/91‑1 (MQ=255)

                                   aTGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAc                                                      <  2:4973255/93‑1 (MQ=255)

                                   aTGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGCCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAc                                                      <  1:3752429/93‑1 (MQ=255)

                                    tGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACg                                                     >  1:6031510/1‑93 (MQ=255)

                                       gcCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCt                                                  >  1:2185528/1‑93 (MQ=255)

                                        ccAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCtt                                                 >  1:6273541/1‑93 (MQ=255)

                                        ccAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCtt                                                 <  2:446295/93‑1 (MQ=255)

                                         cAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTc                                                <  1:3020242/93‑1 (MQ=255)

                                          aGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTcc                                               <  1:6105872/93‑1 (MQ=255)

                                          aGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTcc                                               <  2:4244395/93‑1 (MQ=255)

                                           ggCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCAGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAAGACTTCCg                                              >  2:921630/1‑93 (MQ=255)

                                            gcgcGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCgg                                             <  2:2114564/93‑1 (MQ=255)

                                            gcgcGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCgg                                             <  1:3457509/93‑1 (MQ=255)

                                              gcgTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGtt                                           >  2:2001597/1‑93 (MQ=255)

                                              gcgTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGtt                                           >  2:114810/1‑93 (MQ=255)

                                                 tCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGttatt                                        <  2:3849944/93‑1 (MQ=255)

                                                 tCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGttatt                                        >  1:5487713/1‑93 (MQ=255)

                                                  cAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGttatta                                       <  1:5291523/93‑1 (MQ=255)

                                                  cAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGttatta                                       >  1:4932275/1‑93 (MQ=255)

                                                  cAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGttatta                                       <  1:4909929/93‑1 (MQ=255)

                                                   aGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTtattat                                      <  1:4004161/93‑1 (MQ=255)

                                                   aGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTtattat                                      >  1:3953425/1‑93 (MQ=255)

                                                    gCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTattatt                                     >  2:191108/1‑93 (MQ=255)

                                                    gCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTattatt                                     >  2:4515663/1‑93 (MQ=255)

                                                    gCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTattatt                                     >  2:4710283/1‑93 (MQ=255)

                                                      gTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGc                                   <  2:3034902/93‑1 (MQ=255)

                                                       tATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCg                                  <  2:4910245/93‑1 (MQ=255)

                                                         tCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCggg                                >  2:5495897/1‑93 (MQ=255)

                                                           gCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGaa                              <  1:5114200/93‑1 (MQ=255)

                                                           gCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGaa                              <  2:3309902/93‑1 (MQ=255)

                                                             cAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACg                            >  2:1022320/1‑93 (MQ=255)

                                                              aaaGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGc                           <  2:1351962/93‑1 (MQ=255)

                                                               aaGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCt                          >  2:732416/1‑93 (MQ=255)

                                                               aaGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCt                          >  1:3086263/1‑93 (MQ=255)

                                                                aGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTc                         >  1:4791307/1‑93 (MQ=255)

                                                                aGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTc                         <  1:3402139/93‑1 (MQ=255)

                                                                aGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTc                         <  2:25521/93‑1 (MQ=255)

                                                                 ggCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTcc                        <  2:2889294/93‑1 (MQ=255)

                                                                  gCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCg                       <  1:2510387/93‑1 (MQ=255)

                                                                   cGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCgg                      <  1:1111942/93‑1 (MQ=255)

                                                                         tgtgATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTc                <  2:353296/93‑1 (MQ=255)

                                                                          gtgATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTcc               <  1:5080420/93‑1 (MQ=255)

                                                                          gtgATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTcc               >  1:3266967/1‑93 (MQ=255)

                                                                          gtgATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTcc               <  2:3222502/93‑1 (MQ=255)

                                                                           tgATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCg              >  1:1053311/1‑93 (MQ=255)

                                                                            gATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGc             >  2:3388804/1‑93 (MQ=255)

                                                                               ccGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGc          >  2:2769429/1‑93 (MQ=255)

                                                                                   aCCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGttt      >  1:1412029/1‑93 (MQ=255)

                                                                                    ccTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTc     <  1:6139684/93‑1 (MQ=255)

                                                                                    ccTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTc     <  2:4785476/93‑1 (MQ=255)

                                                                                     cTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCg    >  1:4500887/1‑93 (MQ=255)

                                                                                      tCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGt   <  2:1505292/93‑1 (MQ=255)

                                                                                       cGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTg  >  2:660530/1‑93 (MQ=255)

TGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTG  >  NC_000913/4158887‑4159066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGCCG  >  NC_000913/4158884‑4159069

CCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGC                                                                                              >  SRR4302533.4098266/1‑94 (MQ=60)

 CATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCC                                                                                             >  SRR4302533.3811273/1‑94 (MQ=60)

 CATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCC                                                                                             <  SRR4302533.193436/94‑1 (MQ=60)

  ATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCT                                                                                            >  SRR4302533.6018058/1‑94 (MQ=60)

  ATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCT                                                                                            <  SRR4302533.5999724/94‑1 (MQ=60)

    GGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCC                                                                                          >  SRR4302533.373740/1‑94 (MQ=60)

    GGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCC                                                                                          >  SRR4302533.3762613/1‑94 (MQ=60)

      TTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCAC                                                                                        <  SRR4302533.320998/94‑1 (MQ=60)

       TGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACA                                                                                       <  SRR4302533.3053841/94‑1 (MQ=60)

       TGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACA                                                                                       <  SRR4302533.5655407/94‑1 (MQ=60)

         ATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATT                                                                                     >  SRR4302533.5981522/1‑94 (MQ=60)

         ATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATT                                                                                     <  SRR4302533.4768268/94‑1 (MQ=60)

          TGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTT                                                                                    <  SRR4302533.2848205/94‑1 (MQ=60)

          TGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTT                                                                                    >  SRR4302533.3051062/1‑94 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTAT                                                                                  <  SRR4302533.2865113/94‑1 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTAT                                                                                  >  SRR4302533.2470105/1‑94 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTAT                                                                                  <  SRR4302533.6165798/94‑1 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTAT                                                                                  <  SRR4302533.630694/94‑1 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTAT                                                                                  >  SRR4302533.1405177/1‑94 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTAT                                                                                  <  SRR4302533.5911380/94‑1 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTAT                                                                                  >  SRR4302533.6298796/1‑94 (MQ=60)

            AGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCACGGCCTCCACATTTAT                                                                                  >  SRR4302533.117784/1‑94 (MQ=60)

             GAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATG                                                                                 >  SRR4302533.3190368/1‑94 (MQ=60)

             GAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATG                                                                                 >  SRR4302533.62574/1‑94 (MQ=60)

             GAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATG                                                                                 <  SRR4302533.5594439/94‑1 (MQ=60)

              AGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGG                                                                                <  SRR4302533.6221600/94‑1 (MQ=60)

              AGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGG                                                                                >  SRR4302533.3851524/1‑94 (MQ=60)

              AGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGG                                                                                <  SRR4302533.5931299/94‑1 (MQ=60)

               GCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGA                                                                               <  SRR4302533.3195626/94‑1 (MQ=60)

               GCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGA                                                                               <  SRR4302533.1898427/94‑1 (MQ=60)

                CGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAG                                                                              <  SRR4302533.3237204/94‑1 (MQ=60)

                AGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAG                                                                              <  SRR4302533.3822173/94‑1 (MQ=60)

                 GGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGT                                                                             >  SRR4302533.3668915/1‑94 (MQ=60)

                 GGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGT                                                                             >  SRR4302533.2775882/1‑94 (MQ=60)

                 GGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGT                                                                             <  SRR4302533.3441357/94‑1 (MQ=60)

                 GGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGT                                                                             >  SRR4302533.4332999/1‑94 (MQ=60)

                  GTTTAATGCTACGCCAACTCATGCGCCATGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTT                                                                            >  SRR4302533.5102883/1‑94 (MQ=60)

                  GTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTT                                                                            >  SRR4302533.4020588/1‑94 (MQ=60)

                  GTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTT                                                                            >  SRR4302533.1578084/1‑94 (MQ=60)

                     TAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCAT                                                                         <  SRR4302533.3526574/94‑1 (MQ=60)

                     TAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCAT                                                                         <  SRR4302533.6276889/94‑1 (MQ=60)

                      AATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCCCATTTATGGAGTTCATG                                                                        >  SRR4302533.3753715/1‑94 (MQ=60)

                      AATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATC                                                                        >  SRR4302533.5263307/1‑94 (MQ=60)

                      AATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATC                                                                        >  SRR4302533.2146726/1‑94 (MQ=60)

                       ATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCG                                                                       >  SRR4302533.3084944/1‑94 (MQ=60)

                        TGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCTGCACCTCGGCCTCCACATTTATGGAGTTCATCGG                                                                      <  SRR4302533.691631/94‑1 (MQ=60)

                        TGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGG                                                                      >  SRR4302533.2642636/1‑94 (MQ=60)

                        TGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGG                                                                      <  SRR4302533.1560148/94‑1 (MQ=60)

                         GCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGT                                                                     >  SRR4302533.4149432/1‑94 (MQ=60)

                          CTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTA                                                                    >  SRR4302533.5849109/1‑94 (MQ=60)

                          CTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTA                                                                    <  SRR4302533.4419490/94‑1 (MQ=60)

                          CTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTA                                                                    >  SRR4302533.4490265/1‑94 (MQ=60)

                           TACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAA                                                                   >  SRR4302533.4899169/1‑94 (MQ=60)

                           TACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAA                                                                   >  SRR4302533.2304404/1‑94 (MQ=60)

                            ACTCCAACTCAGGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAAT                                                                  <  SRR4302533.3628324/94‑1 (MQ=60)

                            ACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAAT                                                                  >  SRR4302533.2318545/1‑94 (MQ=60)

                            ACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAAT                                                                  >  SRR4302533.2318705/1‑94 (MQ=60)

                             CGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATA                                                                 <  SRR4302533.3969539/94‑1 (MQ=60)

                              GCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAA                                                                >  SRR4302533.6164494/1‑94 (MQ=60)

                              GCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAA                                                                >  SRR4302533.2936595/1‑94 (MQ=60)

                               CCAGCTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAAT                                                               <  SRR4302533.2230763/94‑1 (MQ=60)

                               CCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAAT                                                               <  SRR4302533.2333786/94‑1 (MQ=60)

                               CCAACTCATGCGCCAGGCGCGTCAGCGTATCACCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAAT                                                               <  SRR4302533.4962329/94‑1 (MQ=60)

                                CAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATC                                                              >  SRR4302533.1538582/1‑94 (MQ=60)

                                CAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATC                                                              <  SRR4302533.1425296/94‑1 (MQ=60)

                                CAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGACTCCACATTTATGGAGTTCATCGGTAATAATC                                                              >  SRR4302533.4372976/1‑94 (MQ=60)

                                  ACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCT                                                            >  SRR4302533.1543619/1‑94 (MQ=60)

                                   CTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTA                                                           <  SRR4302533.2866768/94‑1 (MQ=60)

                                   CTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTA                                                           >  SRR4302533.4947353/1‑94 (MQ=60)

                                   CTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTA                                                           <  SRR4302533.2549173/94‑1 (MQ=60)

                                    TCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAA                                                          >  SRR4302533.5840078/1‑94 (MQ=60)

                                    TCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAA                                                          <  SRR4302533.2954613/94‑1 (MQ=60)

                                    TCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAA                                                          >  SRR4302533.2150704/1‑94 (MQ=60)

                                     CATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAC                                                         <  SRR4302533.2444098/94‑1 (MQ=60)

                                     CATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAC                                                         <  SRR4302533.2885442/94‑1 (MQ=60)

                                     CATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAC                                                         <  SRR4302533.2649875/94‑1 (MQ=60)

                                     CATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAAC                                                         <  SRR4302533.2852046/94‑1 (MQ=60)

                                      ATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACG                                                        >  SRR4302533.6068065/1‑94 (MQ=60)

                                      ATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGCCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACG                                                        <  SRR4302533.3775432/94‑1 (MQ=60)

                                       TGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTTGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGC                                                       <  SRR4302533.1966438/94‑1 (MQ=60)

                                         CGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCT                                                     >  SRR4302533.2199000/1‑94 (MQ=60)

                                          GCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTT                                                    >  SRR4302533.2971548/1‑94 (MQ=60)

                                          GCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTT                                                    >  SRR4302533.6311011/1‑94 (MQ=60)

                                          GCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTT                                                    <  SRR4302533.3998904/94‑1 (MQ=60)

                                           CCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTC                                                   >  SRR4302533.4055169/1‑94 (MQ=60)

                                           CCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGACCTCCTCATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTC                                                   <  SRR4302533.2698096/94‑1 (MQ=60)

                                            CAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATTGAGCTCATCGGTAATAATCCTAACGCCTTCC                                                  >  SRR4302533.5112225/1‑94 (MQ=60)

                                            CAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCC                                                  <  SRR4302533.3038729/94‑1 (MQ=60)

                                            CAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGAGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCC                                                  >  SRR4302533.6169259/1‑94 (MQ=60)

                                             AGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCG                                                 >  SRR4302533.5528696/1‑94 (MQ=60)

                                             AGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCG                                                 >  SRR4302533.5899559/1‑94 (MQ=60)

                                             AGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCG                                                 <  SRR4302533.6142742/94‑1 (MQ=60)

                                             AGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCG                                                 <  SRR4302533.832084/94‑1 (MQ=60)

                                             AGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCG                                                 >  SRR4302533.3398243/1‑94 (MQ=60)

                                             AGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCG                                                 <  SRR4302533.3829527/94‑1 (MQ=60)

                                               GCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGT                                               <  SRR4302533.3478855/94‑1 (MQ=60)

                                                 GCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTA                                             <  SRR4302533.1100738/94‑1 (MQ=60)

                                                  CGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTAT                                            <  SRR4302533.5728446/94‑1 (MQ=60)

                                                  CGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTAT                                            <  SRR4302533.688892/94‑1 (MQ=60)

                                                   GTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCCCCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATT                                           <  SRR4302533.1830905/94‑1 (MQ=60)

                                                   GTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATT                                           >  SRR4302533.5520794/1‑94 (MQ=60)

                                                   GTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATT                                           >  SRR4302533.3467448/1‑94 (MQ=60)

                                                   GTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATT                                           <  SRR4302533.5376128/94‑1 (MQ=60)

                                                    TCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTA                                          <  SRR4302533.3920876/94‑1 (MQ=60)

                                                    TCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTA                                          >  SRR4302533.4962143/1‑94 (MQ=60)

                                                     CAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTAT                                         <  SRR4302533.4939672/94‑1 (MQ=60)

                                                     CAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTAT                                         >  SRR4302533.3977530/1‑94 (MQ=60)

                                                     CAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTAT                                         <  SRR4302533.5323205/94‑1 (MQ=60)

                                                      AGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATT                                        >  SRR4302533.3811705/1‑94 (MQ=60)

                                                      AGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATT                                        <  SRR4302533.4028539/94‑1 (MQ=60)

                                                        CGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGC                                      >  SRR4302533.2126201/1‑94 (MQ=60)

                                                        CGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGC                                      >  SRR4302533.5935839/1‑94 (MQ=60)

                                                        CGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGACCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGC                                      <  SRR4302533.3685722/94‑1 (MQ=60)

                                                         GTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCG                                     <  SRR4302533.5185866/94‑1 (MQ=60)

                                                              GCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAAC                                >  SRR4302533.2257767/1‑94 (MQ=60)

                                                              GCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAAC                                <  SRR4302533.5145108/94‑1 (MQ=60)

                                                              GCCAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAAC                                <  SRR4302533.6102107/94‑1 (MQ=60)

                                                                CAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAAGGC                              <  SRR4302533.4941539/94‑1 (MQ=60)

                                                                CAAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGC                              >  SRR4302533.6122599/1‑94 (MQ=60)

                                                                 AAAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCT                             >  SRR4302533.3105236/1‑94 (MQ=60)

                                                                  AAGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTC                            >  SRR4302533.4820339/1‑94 (MQ=60)

                                                                   AGGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCC                           <  SRR4302533.3423147/94‑1 (MQ=60)

                                                                    GGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCG                          <  SRR4302533.641611/94‑1 (MQ=60)

                                                                    GGCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCG                          <  SRR4302533.3233977/94‑1 (MQ=60)

                                                                    GGCGGGAGTCTGATCCGCACCTCGGCCTCCACATTGATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCG                          <  SRR4302533.3044370/94‑1 (MQ=60)

                                                                     GCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGG                         <  SRR4302533.5469127/94‑1 (MQ=60)

                                                                     GCGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGG                         <  SRR4302533.2525857/94‑1 (MQ=60)

                                                                      CGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGC                        >  SRR4302533.4420270/1‑94 (MQ=60)

                                                                      CGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGC                        <  SRR4302533.1118541/94‑1 (MQ=60)

                                                                      CGGGAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGC                        >  SRR4302533.3818659/1‑94 (MQ=60)

                                                                        gtagaGTGATCCGCACCTCGCCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCAC                      <  SRR4302533.1210817/89‑1 (MQ=60)

                                                                         GAGTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACC                     >  SRR4302533.4637491/1‑94 (MQ=60)

                                                                           GTGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTC                   >  SRR4302533.1793868/1‑94 (MQ=60)

                                                                            TGTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCC                  >  SRR4302533.3287128/1‑94 (MQ=60)

                                                                             GTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCG                 <  SRR4302533.5111126/94‑1 (MQ=60)

                                                                             GTGATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCG                 >  SRR4302533.1059514/1‑94 (MQ=60)

                                                                               GATCCGCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCT               >  SRR4302533.2954955/1‑94 (MQ=60)

                                                                                    GCACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTT          <  SRR4302533.6155752/94‑1 (MQ=60)

                                                                                     CACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTT         >  SRR4302533.1420566/1‑94 (MQ=60)

                                                                                      ACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTC        >  SRR4302533.2877923/1‑94 (MQ=60)

                                                                                      ACCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTC        >  SRR4302533.2908311/1‑94 (MQ=60)

                                                                                       CCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCG       <  SRR4302533.6176664/94‑1 (MQ=60)

                                                                                       CCTCGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCG       >  SRR4302533.4528150/1‑94 (MQ=60)

                                                                                          CGGCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGC    >  SRR4302533.2146170/1‑94 (MQ=60)

                                                                                            GCCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGCCG  >  SRR4302533.3865506/1‑94 (MQ=60)

CCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGCCG  >  NC_000913/4158884‑4159069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`