Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,173,376 A→G noncoding (44/76 nt) thrT → tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,173,3760AG100.0% 791.1 / NA 247noncoding (44/76 nt)thrTtRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (117/130);  total (117/130)

Only 100 of 254 total aligned reads displayed.

CCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCA  >  NC_000913/4173286‑4173464
                                                                                          |                                                                                        
ccAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTgggg                                                                                        >  1:2972789/1‑93 (MQ=255)
ccAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTgggg                                                                                        <  1:5246866/93‑1 (MQ=255)
  aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                      >  1:581206/1‑93 (MQ=255)
  aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                      <  1:483937/93‑1 (MQ=255)
  aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                      <  1:2587442/93‑1 (MQ=255)
  aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                      <  1:909859/93‑1 (MQ=255)
  aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                      <  2:1172573/93‑1 (MQ=255)
   aGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCg                                                                                     >  1:4270307/1‑93 (MQ=255)
       gatgatGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAg                                                                                 >  1:1545729/1‑93 (MQ=255)
       gatgatGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAg                                                                                 >  1:2036983/1‑93 (MQ=255)
         tgatgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGtt                                                                               <  1:1675213/93‑1 (MQ=255)
           atgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCg                                                                             >  1:500217/1‑93 (MQ=255)
             gCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGaa                                                                           <  1:5389842/93‑1 (MQ=255)
             gCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGAGTGGGGTCGGCAGTTCGaa                                                                           <  2:6129012/93‑1 (MQ=255)
               gggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATc                                                                         <  2:5717570/93‑1 (MQ=255)
                 gTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTg                                                                       <  2:4817702/93‑1 (MQ=255)
                  ttCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGc                                                                      <  1:4686162/93‑1 (MQ=255)
                   tCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGcc                                                                     <  2:2287967/93‑1 (MQ=255)
                    cGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCt                                                                    >  2:2638433/1‑93 (MQ=255)
                    cGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCt                                                                    <  1:332266/93‑1 (MQ=255)
                     gATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTa                                                                   <  2:4655572/93‑1 (MQ=255)
                      aTTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAt                                                                  >  2:1453130/1‑93 (MQ=255)
                      aTTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAt                                                                  <  2:938542/93‑1 (MQ=255)
                       ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                 <  1:5949969/93‑1 (MQ=255)
                       ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                 >  2:1945703/1‑93 (MQ=255)
                           cGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGca                                                             <  2:3720889/93‑1 (MQ=255)
                             cTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcacc                                                           >  2:2472509/1‑93 (MQ=255)
                             cTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcacc                                                           <  1:1015433/93‑1 (MQ=255)
                              tGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcacca                                                          >  1:4577664/1‑93 (MQ=255)
                              tGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGGAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcgcca                                                          >  1:2741349/1‑93 (MQ=255)
                                  cGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtc                                                      <  2:1631284/93‑1 (MQ=255)
                                   gCTCCAAGATGTGGTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtct                                                     <  2:4085453/93‑1 (MQ=255)
                                    cTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtctt                                                    <  2:2739122/93‑1 (MQ=255)
                                    cTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtctt                                                    >  1:1860516/1‑93 (MQ=255)
                                     tCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCttt                                                   <  1:5930374/93‑1 (MQ=255)
                                      ccAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCtttt                                                  <  1:5866905/93‑1 (MQ=255)
                                      ccAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCtttt                                                  >  2:3454035/1‑93 (MQ=255)
                                       cAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtc                                                 <  1:2645392/93‑1 (MQ=255)
                                       cAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtc                                                 <  1:6052596/93‑1 (MQ=255)
                                       cAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtc                                                 >  1:3630695/1‑93 (MQ=255)
                                        aaGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtct                                                <  2:2868224/93‑1 (MQ=255)
                                          gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                              <  2:2447310/93‑1 (MQ=255)
                                          gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                              >  2:5941276/1‑93 (MQ=255)
                                          gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                              >  1:4429583/1‑93 (MQ=255)
                                             gtggtgATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCtcctcc                                           >  2:5996675/1‑93 (MQ=255)
                                              tgCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTccc                                          <  1:5621420/93‑1 (MQ=255)
                                                cTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTg                                        <  2:427707/93‑1 (MQ=255)
                                                 tGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGt                                       <  1:1271956/93‑1 (MQ=255)
                                                 tGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGt                                       >  1:3594891/1‑93 (MQ=255)
                                                 tGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGt                                       <  2:5780193/93‑1 (MQ=255)
                                                  gATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtt                                      >  1:3919197/1‑93 (MQ=255)
                                                  gATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtt                                      >  2:1226722/1‑93 (MQ=255)
                                                  gATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtt                                      <  1:5841693/93‑1 (MQ=255)
                                                   atatAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttt                                     >  2:127213/1‑93 (MQ=255)
                                                    tataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                    <  2:5344932/93‑1 (MQ=255)
                                                    tataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                    <  2:3032323/93‑1 (MQ=255)
                                                     ataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttttt                                   >  2:2806292/1‑93 (MQ=255)
                                                       aGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTc                                 <  2:5475118/93‑1 (MQ=255)
                                                       aGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTc                                 >  1:3041322/1‑93 (MQ=255)
                                                        gCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTcc                                <  1:99380/93‑1 (MQ=255)
                                                        gCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTcc                                >  1:3207772/1‑93 (MQ=255)
                                                         cTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCt                               >  2:698259/1‑93 (MQ=255)
                                                         cTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGAGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCt                               <  1:1767723/93‑1 (MQ=255)
                                                          tCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCtt                              >  1:3847274/1‑93 (MQ=255)
                                                           cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                             <  1:2434480/93‑1 (MQ=255)
                                                           cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                             <  2:3886132/93‑1 (MQ=255)
                                                             gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                           >  1:6128406/1‑93 (MQ=255)
                                                             gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                           >  2:1830265/1‑93 (MQ=255)
                                                              ttGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGt                          <  1:3287189/93‑1 (MQ=255)
                                                              ttGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGt                          <  1:5700171/93‑1 (MQ=255)
                                                               tGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGtt                         >  1:4120069/1‑93 (MQ=255)
                                                                 gTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTa                       >  1:1097737/1‑93 (MQ=255)
                                                                    gagCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTg                    >  2:5146585/1‑93 (MQ=255)
                                                                     agCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGc                   <  1:5572290/93‑1 (MQ=255)
                                                                      gcgcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCa                  >  1:5484296/1‑93 (MQ=255)
                                                                      gcgcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCa                  >  2:836380/1‑93 (MQ=255)
                                                                      gcgcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCa                  <  2:3970989/93‑1 (MQ=255)
                                                                        gcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAtt                >  1:3478507/1‑93 (MQ=255)
                                                                         cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc               <  2:5215296/93‑1 (MQ=255)
                                                                         cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc               <  1:3098807/93‑1 (MQ=255)
                                                                         cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc               <  2:4924332/93‑1 (MQ=255)
                                                                          aCCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTca              <  2:1862465/93‑1 (MQ=255)
                                                                          aCCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTca              >  1:1690757/1‑93 (MQ=255)
                                                                           cccTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaa             >  1:2139751/1‑93 (MQ=255)
                                                                           cccTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaa             >  2:555536/1‑93 (MQ=255)
                                                                           cccTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaa             >  2:844870/1‑93 (MQ=255)
                                                                           cccTTGGTAAGGGTGGGGACGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaa             >  1:4074794/1‑93 (MQ=255)
                                                                             cTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaaca           >  1:1330873/1‑93 (MQ=255)
                                                                             cTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaaca           >  2:3064184/1‑93 (MQ=255)
                                                                              ttGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaacaa          <  2:2114308/93‑1 (MQ=255)
                                                                              ttGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaacaa          <  2:4189940/93‑1 (MQ=255)
                                                                               tGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAg         <  1:4777690/93‑1 (MQ=255)
                                                                                ggTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGt        <  1:1526037/93‑1 (MQ=255)
                                                                                 gTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTc       >  2:3918456/1‑93 (MQ=255)
                                                                                  tAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg      <  1:2255812/93‑1 (MQ=255)
                                                                                  tAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg      >  1:49801/1‑93 (MQ=255)
                                                                                   aaGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCgg     <  2:4579382/93‑1 (MQ=255)
                                                                                    aGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCggg    >  2:3196426/1‑93 (MQ=255)
                                                                                     gggTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGc   <  1:783257/93‑1 (MQ=255)
                                                                                      ggTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCa  <  1:6301483/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCA  >  NC_000913/4173286‑4173464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTG  >  NC_000913/4173283‑4173469
                                                                                             |                                                                                             
CTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGG                                                                                               >  SRR4302533.3586051/1‑94 (MQ=60)
 TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGG                                                                                              >  SRR4302533.1823186/1‑94 (MQ=60)
 TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGG                                                                                              <  SRR4302533.2732679/94‑1 (MQ=60)
  TCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGG                                                                                             >  SRR4302533.2990980/1‑94 (MQ=60)
  TCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGG                                                                                             >  SRR4302533.3479880/1‑94 (MQ=60)
   CCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGT                                                                                            <  SRR4302533.5278413/94‑1 (MQ=60)
    CAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTC                                                                                           <  SRR4302533.1435996/94‑1 (MQ=60)
    CAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTC                                                                                           >  SRR4302533.584625/1‑94 (MQ=60)
    CAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTC                                                                                           >  SRR4302533.584626/1‑94 (MQ=60)
    CAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTC                                                                                           >  SRR4302533.1685964/1‑94 (MQ=60)
     AAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCG                                                                                          <  SRR4302533.2603349/94‑1 (MQ=60)
     AAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCG                                                                                          >  SRR4302533.4296012/1‑94 (MQ=60)
     AAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCG                                                                                          <  SRR4302533.486830/94‑1 (MQ=60)
     AAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCG                                                                                          <  SRR4302533.915194/94‑1 (MQ=60)
      AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGG                                                                                         >  SRR4302533.2720580/1‑94 (MQ=60)
         TGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAG                                                                                      >  SRR4302533.2462443/1‑94 (MQ=60)
         TGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAG                                                                                      >  SRR4302533.1555045/1‑94 (MQ=60)
         TGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAG                                                                                      >  SRR4302533.2049544/1‑94 (MQ=60)
         TGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAG                                                                                      <  SRR4302533.5933720/94‑1 (MQ=60)
          GATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGT                                                                                     >  SRR4302533.2164648/1‑94 (MQ=60)
            TGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTC                                                                                   >  SRR4302533.3111252/1‑94 (MQ=60)
            TGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTC                                                                                   <  SRR4302533.1685357/94‑1 (MQ=60)
             GATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCG                                                                                  >  SRR4302533.503192/1‑94 (MQ=60)
              ATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGA                                                                                 >  SRR4302533.1717495/1‑94 (MQ=60)
              ATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGA                                                                                 >  SRR4302533.3952648/1‑94 (MQ=60)
               TGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAA                                                                                >  SRR4302533.3037287/1‑94 (MQ=60)
                GCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAAT                                                                               <  SRR4302533.3355246/94‑1 (MQ=60)
                GCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAAT                                                                               <  SRR4302533.5422230/94‑1 (MQ=60)
                 CGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATC                                                                              >  SRR4302533.968895/1‑94 (MQ=60)
                 CGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATC                                                                              >  SRR4302533.5337007/1‑94 (MQ=60)
                  GTGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCT                                                                             <  SRR4302533.6161359/94‑1 (MQ=60)
                   GGTTCGATTCCCGCTGCCCGCTCCTAGATGTGCTGATATAGCTCAGTTGGTAGTGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTG                                                                            >  SRR4302533.6343036/1‑94 (MQ=60)
                   GGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTG                                                                            >  SRR4302533.4517899/1‑94 (MQ=60)
                    GTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGC                                                                           >  SRR4302533.3635206/1‑94 (MQ=60)
                     TTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCC                                                                          <  SRR4302533.4714550/94‑1 (MQ=60)
                     TTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCC                                                                          <  SRR4302533.5537687/94‑1 (MQ=60)
                      TCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCT                                                                         <  SRR4302533.1060975/94‑1 (MQ=60)
                       CGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTA                                                                        <  SRR4302533.334299/94‑1 (MQ=60)
                       CGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTA                                                                        <  SRR4302533.2981681/94‑1 (MQ=60)
                        GATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAT                                                                       >  SRR4302533.4568736/1‑94 (MQ=60)
                        GATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAT                                                                       <  SRR4302533.4293753/94‑1 (MQ=60)
                        GATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAT                                                                       >  SRR4302533.5901991/1‑94 (MQ=60)
                        GATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAT                                                                       <  SRR4302533.171108/94‑1 (MQ=60)
                         ATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATC                                                                      <  SRR4302533.5098909/94‑1 (MQ=60)
                          TTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCA                                                                     >  SRR4302533.888984/1‑94 (MQ=60)
                          TTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCA                                                                     <  SRR4302533.1165844/94‑1 (MQ=60)
                          TTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCA                                                                     <  SRR4302533.3323001/94‑1 (MQ=60)
                          TTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCA                                                                     <  SRR4302533.5985954/94‑1 (MQ=60)
                            CCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGC                                                                   >  SRR4302533.389987/1‑94 (MQ=60)
                            CCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGC                                                                   <  SRR4302533.4177381/94‑1 (MQ=60)
                             CCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCA                                                                  >  SRR4302533.1404624/1‑94 (MQ=60)
                             CCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCA                                                                  >  SRR4302533.5773939/1‑94 (MQ=60)
                                CTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCA                                                               >  SRR4302533.5520476/1‑94 (MQ=60)
                                CTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCA                                                               <  SRR4302533.1021398/94‑1 (MQ=60)
                                CTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCA                                                               <  SRR4302533.5653119/94‑1 (MQ=60)
                                CTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCA                                                               >  SRR4302533.4605409/1‑94 (MQ=60)
                                CTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGGAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCGCCA                                                               >  SRR4302533.2758159/1‑94 (MQ=60)
                                  GCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACT                                                             <  SRR4302533.4662377/94‑1 (MQ=60)
                                     CGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCT                                                          <  SRR4302533.1623507/94‑1 (MQ=60)
                                     CGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCT                                                          >  SRR4302533.2610939/1‑94 (MQ=60)
                                      GCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTT                                                         <  SRR4302533.2985384/94‑1 (MQ=60)
                                      GCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTT                                                         >  SRR4302533.1871862/1‑94 (MQ=60)
                                       CTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTT                                                        <  SRR4302533.3668066/94‑1 (MQ=60)
                                        TCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTT                                                       >  SRR4302533.5916863/1‑94 (MQ=60)
                                        TCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTT                                                       <  SRR4302533.5966226/94‑1 (MQ=60)
                                         CCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTC                                                      <  SRR4302533.5902368/94‑1 (MQ=60)
                                         CCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTC                                                      >  SRR4302533.3653033/1‑94 (MQ=60)
                                          CAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCT                                                     <  SRR4302533.6089255/94‑1 (MQ=60)
                                          CAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCT                                                     <  SRR4302533.2661647/94‑1 (MQ=60)
                                           AAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTC                                                    >  SRR4302533.3660295/1‑94 (MQ=60)
                                           AAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTC                                                    <  SRR4302533.262455/94‑1 (MQ=60)
                                            AGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCC                                                   >  SRR4302533.4456361/1‑94 (MQ=60)
                                             GATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCT                                                  <  SRR4302533.6347210/94‑1 (MQ=60)
                                               TGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCC                                                <  SRR4302533.1635009/94‑1 (MQ=60)
                                               TGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCC                                                >  SRR4302533.3161261/1‑94 (MQ=60)
                                                 TGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCT                                              <  SRR4302533.5655398/94‑1 (MQ=60)
                                                 TGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCT                                              >  SRR4302533.571699/1‑94 (MQ=60)
                                                   CTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGT                                            >  SRR4302533.3617009/1‑94 (MQ=60)
                                                    TGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTT                                           >  SRR4302533.6290446/1‑94 (MQ=60)
                                                    TGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTT                                           >  SRR4302533.3943132/1‑94 (MQ=60)
                                                    TGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTT                                           <  SRR4302533.1279564/94‑1 (MQ=60)
                                                     GATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTT                                          <  SRR4302533.5877011/94‑1 (MQ=60)
                                                     GATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTT                                          <  SRR4302533.1936759/94‑1 (MQ=60)
                                                      ATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTT                                         <  SRR4302533.1963643/94‑1 (MQ=60)
                                                        ATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTT                                       >  SRR4302533.4814393/1‑94 (MQ=60)
                                                         TAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTC                                      >  SRR4302533.3059936/1‑94 (MQ=60)
                                                          AGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCT                                     >  SRR4302533.2359214/1‑94 (MQ=60)
                                                          AGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCC                                     >  SRR4302533.3227526/1‑94 (MQ=60)
                                                           GCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCT                                    <  SRR4302533.99996/94‑1 (MQ=60)
                                                            CTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTT                                   >  SRR4302533.3870767/1‑94 (MQ=60)
                                                            CTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGAGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTT                                   <  SRR4302533.1778487/94‑1 (MQ=60)
                                                             TCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTC                                  >  SRR4302533.4890487/1‑94 (MQ=60)
                                                             TCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTC                                  >  SRR4302533.4890486/1‑94 (MQ=60)
                                                              CAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCT                                 <  SRR4302533.6105022/94‑1 (MQ=60)
                                                              CAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCT                                 >  SRR4302533.5417839/1‑94 (MQ=60)
                                                              CAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCT                                 >  SRR4302533.970403/1‑94 (MQ=60)
                                                              CAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCT                                 <  SRR4302533.2449518/94‑1 (MQ=60)
                                                               AGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTG                                <  SRR4302533.6046467/94‑1 (MQ=60)
                                                               AGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTG                                >  SRR4302533.6165339/1‑94 (MQ=60)
                                                                 TTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTT                              <  SRR4302533.5975239/94‑1 (MQ=60)
                                                                 TTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTT                              >  SRR4302533.4145089/1‑94 (MQ=60)
                                                                 TTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTT                              <  SRR4302533.5734594/94‑1 (MQ=60)
                                                                 TTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTT                              <  SRR4302533.3307481/94‑1 (MQ=60)
                                                                 TTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTATCTCCTCCCTGTTTTTTCCTTCTGTT                              >  SRR4302533.4145088/1‑94 (MQ=60)
                                                                   GGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTA                            >  SRR4302533.1104239/1‑94 (MQ=60)
                                                                    GTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAT                           <  SRR4302533.3254304/94‑1 (MQ=60)
                                                                    GTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAT                           <  SRR4302533.5493892/94‑1 (MQ=60)
                                                                      AGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTG                         >  SRR4302533.5260053/1‑94 (MQ=60)
                                                                       GAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGC                        <  SRR4302533.2415055/94‑1 (MQ=60)
                                                                        AGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCA                       >  SRR4302533.5517350/1‑94 (MQ=60)
                                                                        AGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCA                       >  SRR4302533.2117704/1‑94 (MQ=60)
                                                                        AGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCA                       <  SRR4302533.5605966/94‑1 (MQ=60)
                                                                         GCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAT                      <  SRR4302533.5866538/94‑1 (MQ=60)
                                                                          CGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATT                     >  SRR4302533.3499970/1‑94 (MQ=60)
                                                                          CGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATT                     >  SRR4302533.2199320/1‑94 (MQ=60)
                                                                          CGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATT                     >  SRR4302533.3619138/1‑94 (MQ=60)
                                                                           GCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTC                    <  SRR4302533.3577765/94‑1 (MQ=60)
                                                                            CACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCA                   <  SRR4302533.3117138/94‑1 (MQ=60)
                                                                            CACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCA                   <  SRR4302533.3117858/94‑1 (MQ=60)
                                                                            CACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCA                   >  SRR4302533.1701000/1‑94 (MQ=60)
                                                                            CACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCA                   >  SRR4302533.3306177/1‑94 (MQ=60)
                                                                             ACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAA                  >  SRR4302533.6043475/1‑94 (MQ=60)
                                                                             ACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAA                  >  SRR4302533.2152940/1‑94 (MQ=60)
                                                                             ACCCTTGGTAAGGGTGGGGACGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAA                  >  SRR4302533.4099601/1‑94 (MQ=60)
                                                                               CCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACA                >  SRR4302533.5675224/1‑94 (MQ=60)
                                                                               CCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACA                >  SRR4302533.1338857/1‑94 (MQ=60)
                                                                                CTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAA               >  SRR4302533.5863192/1‑94 (MQ=60)
                                                                                CTTGGTAAGGGGGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAA               >  SRR4302533.4094376/1‑94 (MQ=60)
                                                                                  TGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCCCCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGT             <  SRR4302533.186405/94‑1 (MQ=60)
                                                                                  TGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGT             <  SRR4302533.5029436/94‑1 (MQ=60)
                                                                                  TGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGT             <  SRR4302533.4806629/94‑1 (MQ=60)
                                                                                   GGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTC            <  SRR4302533.4348314/94‑1 (MQ=60)
                                                                                   GGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTC            <  SRR4302533.1535221/94‑1 (MQ=60)
                                                                                    GTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCG           >  SRR4302533.50143/1‑94 (MQ=60)
                                                                                    GTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCG           <  SRR4302533.4715382/94‑1 (MQ=60)
                                                                                    GTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCG           <  SRR4302533.111961/94‑1 (MQ=60)
                                                                                    GTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCACCGCCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCG           <  SRR4302533.1541272/94‑1 (MQ=60)
                                                                                     TAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGG          <  SRR4302533.2269766/94‑1 (MQ=60)
                                                                                      AAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGG         >  SRR4302533.479566/1‑94 (MQ=60)
                                                                                       AGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGC        >  SRR4302533.1287084/1‑94 (MQ=60)
                                                                                        GGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCA       <  SRR4302533.787863/94‑1 (MQ=60)
                                                                                         GGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAT      <  SRR4302533.6339122/94‑1 (MQ=60)
                                                                                           TGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGT    >  SRR4302533.5378933/1‑94 (MQ=60)
                                                                                            GGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT   >  SRR4302533.4348698/1‑94 (MQ=60)
                                                                                            GGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT   >  SRR4302533.5196371/1‑94 (MQ=60)
                                                                                             GGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTG  >  SRR4302533.3126509/1‑94 (MQ=60)
                                                                                             GGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTG  <  SRR4302533.3407626/94‑1 (MQ=60)
                                                                                             GGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTG  <  SRR4302533.1912523/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTG  >  NC_000913/4173283‑4173469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑