Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,201,958 G→A W354* (TGG→TAG)  hydG → HydG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,201,9580GA100.0% 918.6 / NA 245W354* (TGG→TAG) hydGHydG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (128/117);  total (128/117)

Only 100 of 249 total aligned reads displayed.

GCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGC  >  NC_000913/4201868‑4202047
                                                                                          |                                                                                         
gcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGc                                                                                         >  1:2689030/1‑93 (MQ=255)
gcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGc                                                                                         <  1:1893112/93‑1 (MQ=255)
 ccATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGcc                                                                                        <  2:2924060/93‑1 (MQ=255)
  cATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCg                                                                                       >  2:2698236/1‑93 (MQ=255)
  cATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCg                                                                                       <  1:6136772/93‑1 (MQ=255)
     ttgcTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGa                                                                                    <  2:3223476/90‑1 (MQ=255)
      ttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGaa                                                                                   >  1:917920/1‑93 (MQ=255)
       tCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGaaa                                                                                  <  2:5203117/93‑1 (MQ=255)
       tCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGaaa                                                                                  >  2:5155978/1‑93 (MQ=255)
       tCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGaaa                                                                                  >  1:4954099/1‑93 (MQ=255)
         tGCAGCGCTCTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAata                                                                                <  1:1107550/93‑1 (MQ=255)
           cAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATAtt                                                                              <  1:597650/93‑1 (MQ=255)
            aGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTc                                                                             <  2:2488707/93‑1 (MQ=255)
             gcgcTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCg                                                                            <  2:6235252/93‑1 (MQ=255)
               gcTTTGCCGGGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTg                                                                          <  2:1955585/93‑1 (MQ=255)
                 tttGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAg                                                                        >  2:685711/1‑93 (MQ=255)
                 tttGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAg                                                                        <  2:1424018/93‑1 (MQ=255)
                   tGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCt                                                                      <  1:3505015/93‑1 (MQ=255)
                   tGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCt                                                                      <  2:1672619/93‑1 (MQ=255)
                    gCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTg                                                                     <  2:924635/93‑1 (MQ=255)
                    gCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTg                                                                     >  1:2638325/1‑93 (MQ=255)
                      cGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGa                                                                   >  2:1738168/1‑93 (MQ=255)
                      cGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGa                                                                   >  2:1577517/1‑93 (MQ=255)
                       gAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGaa                                                                  >  2:612156/1‑93 (MQ=255)
                        aGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGaaa                                                                 >  1:3728164/1‑93 (MQ=255)
                           gTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAcg                                                              <  1:4853763/93‑1 (MQ=255)
                             aaTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAcgcg                                                            <  1:4024709/93‑1 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           <  2:2073758/93‑1 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           <  1:1355357/93‑1 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           >  2:2132584/1‑93 (MQ=255)
                              aTCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCgg                                                           >  2:1357355/1‑93 (MQ=255)
                               tCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGt                                                          <  2:411038/93‑1 (MQ=255)
                                 gCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTgg                                                        <  2:3741825/93‑1 (MQ=255)
                                  cAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGa                                                       <  1:1512925/93‑1 (MQ=255)
                                   aaGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGaa                                                      >  2:600064/1‑93 (MQ=255)
                                   aaGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGaa                                                      >  1:5253773/1‑93 (MQ=255)
                                     ggCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACg                                                    <  2:4969846/93‑1 (MQ=255)
                                     ggCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACg                                                    <  1:1938545/93‑1 (MQ=255)
                                      gCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACgg                                                   >  2:2032154/1‑93 (MQ=255)
                                        ggTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGc                                                 >  1:2778270/1‑93 (MQ=255)
                                         gTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCa                                                <  2:2770828/93‑1 (MQ=255)
                                          tAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAg                                               <  1:1011253/93‑1 (MQ=255)
                                          tAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAg                                               >  1:2149045/1‑93 (MQ=255)
                                           aaaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgt                                              <  2:3402161/93‑1 (MQ=255)
                                           aaaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgt                                              >  2:5541248/1‑93 (MQ=255)
                                           aaaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgt                                              >  2:5973052/1‑93 (MQ=255)
                                           aaaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgg                                              >  2:570212/1‑92 (MQ=255)
                                            aaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtg                                             <  2:5636728/93‑1 (MQ=255)
                                            aaaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtg                                             >  1:2541971/1‑93 (MQ=255)
                                             aaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtgg                                            >  1:5451154/1‑93 (MQ=255)
                                             aaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtgg                                            >  2:4292678/1‑93 (MQ=255)
                                               ggTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtggtg                                          >  1:2023339/1‑93 (MQ=255)
                                                gTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgc                                         <  2:1251848/93‑1 (MQ=255)
                                                 ttttACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgct                                        >  2:3861471/1‑93 (MQ=255)
                                                      cGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCGGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGa                                   <  1:2942398/93‑1 (MQ=255)
                                                       gCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAc                                  <  2:5388026/93‑1 (MQ=255)
                                                       gCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAc                                  <  2:511839/93‑1 (MQ=255)
                                                          ccAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCgg                               >  2:3803972/1‑93 (MQ=255)
                                                          ccAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCgg                               >  1:5156663/1‑93 (MQ=255)
                                                           cAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCggg                              >  1:1264798/1‑93 (MQ=255)
                                                            aGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCgggg                             >  2:829775/1‑93 (MQ=255)
                                                              gCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGaa                           >  1:2988438/1‑93 (MQ=255)
                                                                  tGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatata                       >  1:5912494/1‑93 (MQ=255)
                                                                  tGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatata                       >  1:1195077/1‑93 (MQ=255)
                                                                  tGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatata                       >  2:3278830/1‑93 (MQ=255)
                                                                  tGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatata                       >  2:2658392/1‑93 (MQ=255)
                                                                   ggATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAatatat                      <  1:381683/93‑1 (MQ=255)
                                                                    gATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAtt                     >  2:1474068/1‑93 (MQ=255)
                                                                    gATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCAGCTGACCGGGGAATATAtt                     <  2:6135046/93‑1 (MQ=255)
                                                                     aTCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAttt                    >  1:5670415/1‑93 (MQ=255)
                                                                     aTCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAttt                    >  2:4567854/1‑93 (MQ=255)
                                                                     aTCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAttt                    <  1:6202157/93‑1 (MQ=255)
                                                                     aTCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAttt                    >  1:3307039/1‑93 (MQ=255)
                                                                     aTCTGTTGATTCATTACGACTAGCCGGGAAACATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAttt                    <  1:631670/93‑1 (MQ=255)
                                                                      tCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTc                   >  2:5822716/1‑93 (MQ=255)
                                                                      tCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTc                   >  1:4545727/1‑93 (MQ=255)
                                                                      tCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTc                   >  1:4464640/1‑93 (MQ=255)
                                                                      tCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTc                   >  1:4089572/1‑93 (MQ=255)
                                                                        tGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCg                 >  1:2262546/1‑93 (MQ=255)
                                                                         gTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGa                >  2:3471197/1‑93 (MQ=255)
                                                                         gTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGa                >  1:5745434/1‑93 (MQ=255)
                                                                         gTTGAATCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGa                >  2:3988987/1‑93 (MQ=255)
                                                                          ttGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGaa               <  2:2393344/93‑1 (MQ=255)
                                                                          ttGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGaa               <  2:2278965/93‑1 (MQ=255)
                                                                           tGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAc              <  2:363226/93‑1 (MQ=255)
                                                                            gagtCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcg             <  1:3790944/90‑1 (MQ=255)
                                                                             aTTCATTACGACTAGCGGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcgc            <  1:2234192/93‑1 (MQ=255)
                                                                             aTTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcgc            <  1:4692752/93‑1 (MQ=255)
                                                                              ttCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcgcg           >  1:2502172/1‑93 (MQ=255)
                                                                              ttCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcgcg           <  1:1530368/93‑1 (MQ=255)
                                                                               tCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGa          <  2:2825269/93‑1 (MQ=255)
                                                                                 aTTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGc        >  2:1798152/1‑93 (MQ=255)
                                                                                 aTTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGc        >  1:78636/1‑93 (MQ=255)
                                                                                  ttACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCt       <  2:1270171/93‑1 (MQ=255)
                                                                                  ttACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCt       >  1:5011621/1‑93 (MQ=255)
                                                                                  ttACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCt       <  2:728880/93‑1 (MQ=255)
                                                                                  ttACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGGGGAACGGGCAGTGGTGGTGCTTGCCGGGGGATATATTTTTCAACGCCAGcg       >  1:2803465/1‑92 (MQ=25)
                                                                                    aCGACTAGCCGGGAAATATTCGTGAGCTGGAAAGCGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGc     <  2:905944/93‑1 (MQ=255)
                                                                                    aCGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGc     >  1:3581607/1‑93 (MQ=255)
                                                                                       aCTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAGCGCGAGCTGCCGc  >  2:4359617/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGC  >  NC_000913/4201868‑4202047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGC  >  NC_000913/4201865‑4202051
                                                                                             |                                                                                             
CTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTA                                                                                               >  SRR4302533.107038/1‑94 (MQ=60)
  GGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGC                                                                                             <  SRR4302533.4677601/94‑1 (MQ=60)
  GGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGC                                                                                             >  SRR4302533.2705515/1‑94 (MQ=60)
   GCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCC                                                                                            <  SRR4302533.1904671/94‑1 (MQ=60)
    CCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCG                                                                                           >  SRR4302533.4609612/1‑94 (MQ=60)
     CATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGG                                                                                          <  SRR4302533.6173743/94‑1 (MQ=60)
     CATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGG                                                                                          <  SRR4302533.5116734/94‑1 (MQ=60)
      ATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGG                                                                                         <  SRR4302533.4398884/94‑1 (MQ=60)
      ATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGG                                                                                         >  SRR4302533.4124029/1‑94 (MQ=60)
        TTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCTGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAA                                                                                       <  SRR4302533.5558381/94‑1 (MQ=60)
        TTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAA                                                                                       >  SRR4302533.923313/1‑94 (MQ=60)
         TTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAA                                                                                      >  SRR4302533.4984109/1‑94 (MQ=60)
            TGCAGCGCTCTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATAT                                                                                   <  SRR4302533.1114116/94‑1 (MQ=60)
             GCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATT                                                                                  <  SRR4302533.675813/94‑1 (MQ=60)
             GCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATT                                                                                  <  SRR4302533.2018101/94‑1 (MQ=60)
              CAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTC                                                                                 <  SRR4302533.601173/94‑1 (MQ=60)
                 GGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGGCTAGCCGGGAAATATTCGTG                                                                              <  SRR4302533.138732/94‑1 (MQ=60)
                  GCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGA                                                                             >  SRR4302533.5733000/1‑94 (MQ=60)
                   CTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAG                                                                            >  SRR4302533.4952214/1‑94 (MQ=60)
                   CTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAG                                                                            >  SRR4302533.2280820/1‑94 (MQ=60)
                   CTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAG                                                                            <  SRR4302533.5977636/94‑1 (MQ=60)
                     TTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCT                                                                          <  SRR4302533.2812202/94‑1 (MQ=60)
                     TTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCCGTTGATTCATTACGACTAGCCGGGAAATATTCGTGGGCT                                                                          <  SRR4302533.3164768/94‑1 (MQ=60)
                      TGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTG                                                                         >  SRR4302533.2654541/1‑94 (MQ=60)
                      TGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTG                                                                         <  SRR4302533.69958/94‑1 (MQ=60)
                      TGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTG                                                                         <  SRR4302533.3526623/94‑1 (MQ=60)
                        CCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGA                                                                       >  SRR4302533.1206274/1‑94 (MQ=60)
                          GAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAA                                                                     >  SRR4302533.3751034/1‑94 (MQ=60)
                            GCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAAC                                                                   <  SRR4302533.4037978/94‑1 (MQ=60)
                              GTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGC                                                                 >  SRR4302533.6218052/1‑94 (MQ=60)
                              GTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGC                                                                 <  SRR4302533.4883177/94‑1 (MQ=60)
                                AATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGG                                                               <  SRR4302533.153003/94‑1 (MQ=60)
                                AATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGG                                                               <  SRR4302533.4049211/94‑1 (MQ=60)
                                AATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGG                                                               <  SRR4302533.2573510/94‑1 (MQ=60)
                                 ATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGT                                                              <  SRR4302533.1363503/94‑1 (MQ=60)
                                 ATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGT                                                              >  SRR4302533.1277770/1‑94 (MQ=60)
                                  TCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTG                                                             <  SRR4302533.3539852/94‑1 (MQ=60)
                                    GCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGA                                                           >  SRR4302533.4023659/1‑94 (MQ=60)
                                     CAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAA                                                          >  SRR4302533.5285338/1‑94 (MQ=60)
                                     CAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAA                                                          <  SRR4302533.1522039/94‑1 (MQ=60)
                                      AAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAAC                                                         <  SRR4302533.1906952/94‑1 (MQ=60)
                                      AAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAAC                                                         <  SRR4302533.2283704/94‑1 (MQ=60)
                                        GGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGG                                                       >  SRR4302533.1121679/1‑94 (MQ=60)
                                        GGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGG                                                       <  SRR4302533.1950365/94‑1 (MQ=60)
                                         GCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGG                                                      <  SRR4302533.5347686/94‑1 (MQ=60)
                                          CGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGC                                                     <  SRR4302533.273980/94‑1 (MQ=60)
                                          CGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGC                                                     >  SRR4302533.2795336/1‑94 (MQ=60)
                                           GGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCA                                                    >  SRR4302533.6073672/1‑94 (MQ=60)
                                            GTACAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAG                                                   <  SRR4302533.5045456/94‑1 (MQ=60)
                                            GTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAG                                                   >  SRR4302533.5472054/1‑94 (MQ=60)
                                            GTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAG                                                   >  SRR4302533.2162279/1‑94 (MQ=60)
                                             TAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGT                                                  <  SRR4302533.1017170/94‑1 (MQ=60)
                                              AAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTG                                                 <  SRR4302533.34964/94‑1 (MQ=60)
                                              AAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTG                                                 >  SRR4302533.2557618/1‑94 (MQ=60)
                                              AAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTG                                                 <  SRR4302533.2782919/94‑1 (MQ=60)
                                               AAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGG                                                >  SRR4302533.2190624/1‑94 (MQ=60)
                                               AAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGG                                                >  SRR4302533.5483993/1‑94 (MQ=60)
                                               AAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGG                                                <  SRR4302533.198497/94‑1 (MQ=60)
                                                 AGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTG                                              <  SRR4302533.3667073/94‑1 (MQ=60)
                                                 AGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTG                                              >  SRR4302533.2035834/1‑94 (MQ=60)
                                                       TACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTG                                        <  SRR4302533.3577993/94‑1 (MQ=60)
                                                         CGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCGGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAC                                      <  SRR4302533.2960364/94‑1 (MQ=60)
                                                         CGCCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAC                                      <  SRR4302533.5034866/94‑1 (MQ=60)
                                                           CCCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCG                                    >  SRR4302533.5193771/1‑94 (MQ=60)
                                                            CCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGG                                   >  SRR4302533.5187798/1‑94 (MQ=60)
                                                            CCCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGGGGGACGTGCAGTGGTGCTGCTGACCGG                                   >  SRR4302533.2702467/1‑94 (MQ=60)
                                                             CCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGG                                  >  SRR4302533.2113694/1‑94 (MQ=60)
                                                             CCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGG                                  >  SRR4302533.1272366/1‑94 (MQ=60)
                                                             CCAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGG                                  >  SRR4302533.4079268/1‑94 (MQ=60)
                                                             CCAGGAGATGGATCTGTTGATTCATTACGACTAGACGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGG                                  >  SRR4302533.3815055/1‑94 (MQ=60)
                                                              CAGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGG                                 >  SRR4302533.3762403/1‑94 (MQ=60)
                                                               AGGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGA                                >  SRR4302533.1227481/1‑94 (MQ=60)
                                                                GGCGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAA                               >  SRR4302533.3006721/1‑94 (MQ=60)
                                                                 ggggttgttgttttatgTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAAT                              <  SRR4302533.3384828/77‑1 (MQ=60)
                                                                  CGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATA                             <  SRR4302533.4939021/94‑1 (MQ=60)
                                                                  CGATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATA                             >  SRR4302533.2176231/1‑94 (MQ=60)
                                                                    ATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATA                           >  SRR4302533.5948220/1‑94 (MQ=60)
                                                                    ATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATA                           >  SRR4302533.5948221/1‑94 (MQ=60)
                                                                    ATGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATA                           >  SRR4302533.1202262/1‑94 (MQ=60)
                                                                     TGGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAT                          <  SRR4302533.1890698/94‑1 (MQ=60)
                                                                      GGATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATT                         <  SRR4302533.383985/94‑1 (MQ=60)
                                                                       GATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTT                        >  SRR4302533.5704662/1‑94 (MQ=60)
                                                                       GATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTT                        <  SRR4302533.6329317/94‑1 (MQ=60)
                                                                       GATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTT                        >  SRR4302533.885751/1‑94 (MQ=60)
                                                                       GATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTT                        >  SRR4302533.3327461/1‑94 (MQ=60)
                                                                        ATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTC                       >  SRR4302533.4573258/1‑94 (MQ=60)
                                                                        ATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTC                       >  SRR4302533.4491644/1‑94 (MQ=60)
                                                                        ATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTC                       >  SRR4302533.4114470/1‑94 (MQ=60)
                                                                        ATCTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTC                       <  SRR4302533.6239355/94‑1 (MQ=60)
                                                                        ATCTGTTGATTCATTACGACTAGCCGGGAAACATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTC                       <  SRR4302533.635390/94‑1 (MQ=60)
                                                                          CTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCG                     >  SRR4302533.2829152/1‑94 (MQ=60)
                                                                          CTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCG                     >  SRR4302533.2270629/1‑94 (MQ=60)
                                                                          CTGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCG                     >  SRR4302533.2276541/1‑94 (MQ=60)
                                                                           TGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGA                    >  SRR4302533.4721477/1‑94 (MQ=60)
                                                                           TGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGA                    >  SRR4302533.5780104/1‑94 (MQ=60)
                                                                           TGTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGA                    >  SRR4302533.2984270/1‑94 (MQ=60)
                                                                            GTTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAA                   >  SRR4302533.3255621/1‑94 (MQ=60)
                                                                             TTGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAC                  <  SRR4302533.1429367/94‑1 (MQ=60)
                                                                              TGATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACG                 <  SRR4302533.5727599/94‑1 (MQ=60)
                                                                               GAGTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGC                <  SRR4302533.3814156/94‑1 (MQ=60)
                                                                                ATTCATTACGACTAGCGGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCG               <  SRR4302533.2247976/94‑1 (MQ=60)
                                                                                ATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCG               <  SRR4302533.4721192/94‑1 (MQ=60)
                                                                                ATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCG               >  SRR4302533.1637862/1‑94 (MQ=60)
                                                                                ATTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCG               >  SRR4302533.2517604/1‑94 (MQ=60)
                                                                                 TTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGA              <  SRR4302533.1539568/94‑1 (MQ=60)
                                                                                 TTCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGA              <  SRR4302533.2947098/94‑1 (MQ=60)
                                                                                  TCATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAG             <  SRR4302533.4652100/94‑1 (MQ=60)
                                                                                   CATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGC            >  SRR4302533.79140/1‑94 (MQ=60)
                                                                                    ATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCT           >  SRR4302533.6010695/1‑94 (MQ=60)
                                                                                    ATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCT           >  SRR4302533.3781600/1‑94 (MQ=60)
                                                                                    ATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCT           >  SRR4302533.5041986/1‑94 (MQ=60)
                                                                                    ATTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGGGGAACGGGCAGTGGTGGTGCTTGCCGGGGGATATATTTttcaacgccagcg           >  SRR4302533.2820717/1‑81 (MQ=60)
                                                                                     TTACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTG          >  SRR4302533.4570786/1‑94 (MQ=60)
                                                                                      TACGACTAGCCGGGAAATATTCGTGAGCTGGAAAGCGCGGTGTAACTGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGC         <  SRR4302533.2583419/94‑1 (MQ=60)
                                                                                      TACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGC         >  SRR4302533.3603670/1‑94 (MQ=60)
                                                                                       ACGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCC        <  SRR4302533.6189764/94‑1 (MQ=60)
                                                                                        CGACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCG       >  SRR4302533.1104579/1‑94 (MQ=60)
                                                                                         GACTAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGC      >  SRR4302533.251801/1‑94 (MQ=60)
                                                                                            TAGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGG   >  SRR4302533.2092257/1‑94 (MQ=60)
                                                                                             AGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGCCCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGC  <  SRR4302533.5829577/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGC  >  NC_000913/4201865‑4202051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑