Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,203,421	A→G	V30A (GTT→GCT)	purD ←	phosphoribosylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,203,421	0	A	G	99.5%	666.5 / ‑5.6	207	V30A (GTT→GCT)	purD	phosphoribosylglycinamide synthetase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (107/99); total (107/100)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 225 total aligned reads displayed.

GCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAAC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATC  >  NC_000913/4203331‑4203510

   aaaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑aaaa                                                                                      >  2:5021541/1‑93 (MQ=255)

    aaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑aaaaa                                                                                     >  1:1315774/1‑93 (MQ=255)

     aTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAAc                                                                                    <  1:3536061/93‑1 (MQ=255)

       ccAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAg                                                                                  <  2:3454039/93‑1 (MQ=255)

        cAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGt                                                                                 <  1:1426638/93‑1 (MQ=255)

        cAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGt                                                                                 >  1:5200056/1‑93 (MQ=255)

        cAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGt                                                                                 <  1:3880644/93‑1 (MQ=255)

         aaCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGtc                                                                                <  1:409542/93‑1 (MQ=255)

         aaCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGtc                                                                                >  1:3087175/1‑93 (MQ=255)

          aCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGtct                                                                               >  1:2049191/1‑93 (MQ=255)

          aCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGtct                                                                               <  2:3892355/93‑1 (MQ=255)

           cAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGtctc                                                                              <  1:433638/93‑1 (MQ=255)

           cAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGtctc                                                                              >  2:4188824/1‑93 (MQ=255)

            aGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCa                                                                             <  1:5258325/93‑1 (MQ=255)

             gcgcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCaa                                                                            >  1:4514508/1‑93 (MQ=255)

              cgcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAAc                                                                           <  1:4665514/93‑1 (MQ=255)

              cgcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAAc                                                                           >  2:1545977/1‑93 (MQ=255)

               gcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAAcc                                                                          >  1:5946257/1‑93 (MQ=255)

               gcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAAcc                                                                          <  2:3925115/93‑1 (MQ=255)

                ccGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCa                                                                         <  1:2960848/93‑1 (MQ=255)

                 cGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAg                                                                        <  2:2816453/93‑1 (MQ=255)

                  gggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAgc                                                                       >  1:3669440/1‑93 (MQ=255)

                  gggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAgc                                                                       >  2:1351490/1‑93 (MQ=255)

                   ggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAgcg                                                                      <  2:464697/93‑1 (MQ=255)

                   ggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAgcg                                                                      >  2:5249231/1‑93 (MQ=255)

                   ggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAgcg                                                                      >  1:4582894/1‑93 (MQ=255)

                     atatCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAgcggc                                                                    >  2:383020/1‑93 (MQ=255)

                     atatCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAgcggc                                                                    >  1:6039577/1‑93 (MQ=255)

                        tCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGAc                                                                 >  2:3003540/1‑93 (MQ=255)

                        tCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGAc                                                                 <  1:1872997/93‑1 (MQ=255)

                          ggTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTg                                                               <  1:629133/93‑1 (MQ=255)

                          ggTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTACCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTg                                                               >  2:3263140/1‑93 (MQ=255)

                           gTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTgg                                                              >  1:3448386/1‑93 (MQ=255)

                              aCGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGcc                                                           <  2:2762246/93‑1 (MQ=255)

                              aCGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGcc                                                           >  1:1784754/1‑93 (MQ=255)

                               cGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCg                                                          <  2:4895358/93‑1 (MQ=255)

                                 ccAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCt                                                        >  1:1162255/1‑93 (MQ=255)

                                  cAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCtt                                                       >  2:573361/1‑93 (MQ=255)

                                   aaTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCttt                                                      >  2:3113161/1‑93 (MQ=255)

                                   aaTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCttt                                                      >  2:5955177/1‑93 (MQ=255)

                                    aTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTc                                                     <  2:5110312/93‑1 (MQ=255)

                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    >  2:6154325/1‑93 (MQ=255)

                                      aGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCa                                                   <  1:5882311/93‑1 (MQ=255)

                                      aGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCa                                                   <  2:5699865/93‑1 (MQ=255)

                                      aGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCa                                                   <  1:636000/93‑1 (MQ=255)

                                       gCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAg                                                  >  1:4713118/1‑93 (MQ=255)

                                        cAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAgg                                                 >  2:5502345/1‑93 (MQ=255)

                                         aaCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTGCCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGc                                                <  1:2756434/93‑1 (MQ=255)

                                         aaCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGc                                                <  1:5589567/93‑1 (MQ=255)

                                          aCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGcc                                               >  2:439781/1‑93 (MQ=255)

                                          aCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGcc                                               >  1:5705612/1‑93 (MQ=255)

                                          aCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGcc                                               >  1:5652160/1‑93 (MQ=255)

                                             ttttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgc                                            <  1:2569664/93‑1 (MQ=255)

                                             ttttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgc                                            >  1:200297/1‑93 (MQ=255)

                                             ttttGCAGCGCGGGCTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCTACTGGGCCGCTTTCCAGGCCAgc                                            >  2:6307042/1‑93 (MQ=255)

                                              tttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgcg                                           <  1:778901/93‑1 (MQ=255)

                                                 gCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGt                                        <  2:2720294/93‑1 (MQ=255)

                                                 gCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGt                                        <  2:2173753/93‑1 (MQ=255)

                                                  cAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTg                                       <  1:4466688/93‑1 (MQ=255)

                                                    gcgcGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCt                                     <  1:3188091/93‑1 (MQ=255)

                                                       cgGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTcgc                                  >  2:1898278/1‑93 (MQ=255)

                                                       cgGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTcgc                                  >  1:5734559/1‑93 (MQ=255)

                                                         ggTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCgcgc                                >  2:718017/1‑93 (MQ=255)

                                                          gTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcc                               <  2:166689/93‑1 (MQ=255)

                                                           ttCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgccc                              <  2:579180/93‑1 (MQ=255)

                                                            tCCAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccg                             >  2:3541897/1‑93 (MQ=255)

                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            <  2:2162823/93‑1 (MQ=255)

                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            >  1:5895801/1‑93 (MQ=255)

                                                              cagtgcagtgCCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgcc                           >  1:1061027/1‑93 (MQ=255)

                                                              cagtgcagtcCCTGCATTCCCCGGAGCAGC‑AAAAACAGTCTCACCCAGCGGCGCCTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgcc                           <  2:84316/93‑1 (MQ=255)

                                                               agtgcagtgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCccgccg                          >  1:4046712/1‑93 (MQ=255)

                                                                gtgcagtgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         <  1:4207445/93‑1 (MQ=255)

                                                                gtgcagtgcCTGCATTACCCGGAGCAAC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         <  1:4954716/93‑1 (MQ=255)

                                                                 tgcagtgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGtt                        <  2:4093095/93‑1 (MQ=255)

                                                                  gcagtgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTa                       >  2:3723921/1‑93 (MQ=255)

                                                                   cagtgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTAc                      >  2:2366622/1‑93 (MQ=255)

                                                                    agtccCTGCATTCCCCGGAGCAGA‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTAcc                     <  2:1097021/93‑1 (MQ=255)

                                                                     gtgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCa                    >  2:3949909/1‑93 (MQ=255)

                                                                      tgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCaa                   <  1:977358/93‑1 (MQ=255)

                                                                      tgcCTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGCTACCaa                   >  1:2349771/1‑93 (MQ=255)

                                                                       gcCTGCATTACCCGGAGCAGA‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAAt                  <  2:6207051/93‑1 (MQ=255)

                                                                         cTGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCa                <  1:925804/93‑1 (MQ=255)

                                                                          tGCATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAc               >  1:1257105/1‑93 (MQ=255)

                                                                          tGCATTACCCGGAGCAGC‑AAAAACAGTCCCAACCAGCGGCGACTGGGCCGCTTTCCAGCCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAc               <  1:690733/93‑1 (MQ=255)

                                                                            cATTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTa             >  1:3251867/1‑93 (MQ=255)

                                                                             aTTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGTCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTaa            <  1:500806/93‑1 (MQ=255)

                                                                             aTTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACtca            >  2:5421755/1‑91 (MQ=255)

                                                                             aTTACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTaa            <  1:2322338/93‑1 (MQ=255)

                                                                              ttACCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGCCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAAt           <  1:1768780/93‑1 (MQ=255)

                                                                               ttcccGGAGGAAGAAAAAACAGTCTCAACCGGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAAt           <  2:5306389/91‑1 (MQ=255)

                                                                                aCCCGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATAc         >  1:2114674/1‑93 (MQ=255)

                                                                                 cccGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACt        <  1:3835270/93‑1 (MQ=255)

                                                                                  ccGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACtt       >  1:4329461/1‑93 (MQ=255)

                                                                                   cGGAGCAGC‑AAAAACAGTCTCACCCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACttt      <  2:2481129/93‑1 (MQ=255)

                                                                                   cGGAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACttt      >  2:5853778/1‑93 (MQ=255)

                                                                                   cGGAGCAGC‑AAAAACAGTCCAACCCAGCGGCGACGGGGCCGCTTCCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACttt      <  2:30118/93‑1 (MQ=255)

                                                                                    ggAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTc     <  1:3703237/93‑1 (MQ=255)

                                                                                    ggAGCAGC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTc     >  2:1069995/1‑93 (MQ=255)

                                                                                      agcagc‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCAt   >  2:2226558/1‑93 (MQ=255)

                                                                                       gcagc‑aAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATc  >  1:156650/1‑93 (MQ=255)

GCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAAC‑AAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATC  >  NC_000913/4203331‑4203510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTGCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAACAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATCTAT  >  NC_000913/4203328‑4203513

TGTGCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAG                                                                                              >  SRR4302533.1764503/1‑94 (MQ=60)

TGTGCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAG                                                                                              <  SRR4302533.1693206/94‑1 (MQ=60)

 GTGCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGC                                                                                             <  SRR4302533.2675646/94‑1 (MQ=60)

  TGCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCA                                                                                            <  SRR4302533.1333520/94‑1 (MQ=60)

  TGCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGAAGTGCCTGCATTACCCGGAGCAGCA                                                                                            >  SRR4302533.70193/1‑94 (MQ=60)

      AAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAA                                                                                        >  SRR4302533.1323667/1‑94 (MQ=60)

        ATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACA                                                                                      <  SRR4302533.3557854/94‑1 (MQ=60)

         TCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAG                                                                                     >  SRR4302533.372377/1‑94 (MQ=60)

          CCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGT                                                                                    >  SRR4302533.5231405/1‑94 (MQ=60)

          CCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGT                                                                                    >  SRR4302533.1858374/1‑94 (MQ=60)

           CAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTC                                                                                   <  SRR4302533.3904325/94‑1 (MQ=60)

           CAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTC                                                                                   >  SRR4302533.590236/1‑94 (MQ=60)

           CAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTC                                                                                   <  SRR4302533.1435261/94‑1 (MQ=60)

           CAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTC                                                                                   <  SRR4302533.2774069/94‑1 (MQ=60)

           CAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTC                                                                                   <  SRR4302533.5943254/94‑1 (MQ=60)

           CAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTC                                                                                   >  SRR4302533.3106156/1‑94 (MQ=60)

            AACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCT                                                                                  <  SRR4302533.4652895/94‑1 (MQ=60)

            AACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCT                                                                                  <  SRR4302533.411999/94‑1 (MQ=60)

            AACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCT                                                                                  >  SRR4302533.2061831/1‑94 (MQ=60)

              CAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCA                                                                                <  SRR4302533.436224/94‑1 (MQ=60)

               AGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAA                                                                               >  SRR4302533.2227932/1‑94 (MQ=60)

               AGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAA                                                                               <  SRR4302533.5289898/94‑1 (MQ=60)

               AGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAA                                                                               >  SRR4302533.4541845/1‑94 (MQ=60)

                GCTCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAAC                                                                              <  SRR4302533.1837661/94‑1 (MQ=60)

                 CGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACC                                                                             >  SRR4302533.5982218/1‑94 (MQ=60)

                 CGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACC                                                                             <  SRR4302533.4693779/94‑1 (MQ=60)

                 CGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAACGCGGGTTCCAGTGCAGTGCCTGCATTCCCCGGAGCAGCAAAAACAGTCTAAACC                                                                             >  SRR4302533.556420/1‑94 (MQ=60)

                   CCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAG                                                                           <  SRR4302533.2978947/94‑1 (MQ=60)

                    CGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGC                                                                          >  SRR4302533.3691997/1‑94 (MQ=60)

                    CGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGC                                                                          >  SRR4302533.5112656/1‑94 (MQ=60)

                     GGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCG                                                                         >  SRR4302533.1922226/1‑94 (MQ=60)

                     GGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCG                                                                         >  SRR4302533.4610661/1‑94 (MQ=60)

                     GGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCG                                                                         <  SRR4302533.1352339/94‑1 (MQ=60)

                     GGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCG                                                                         >  SRR4302533.2345710/1‑94 (MQ=60)

                     GGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCG                                                                         >  SRR4302533.1127521/1‑94 (MQ=60)

                      GGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGG                                                                        <  SRR4302533.802095/94‑1 (MQ=60)

                       GATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGC                                                                       <  SRR4302533.2093248/94‑1 (MQ=60)

                       GATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGC                                                                       >  SRR4302533.6076175/1‑94 (MQ=60)

                        ATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCG                                                                      >  SRR4302533.1002086/1‑94 (MQ=60)

                        ATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCG                                                                      >  SRR4302533.1622589/1‑94 (MQ=60)

                        ATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCG                                                                      >  SRR4302533.5615798/1‑94 (MQ=60)

                           TCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACT                                                                   <  SRR4302533.5710517/94‑1 (MQ=60)

                           TCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACT                                                                   <  SRR4302533.1884414/94‑1 (MQ=60)

                            CGGTCACGCCAATAGCAACGTTTTGCCGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAACAACAGTCTCAACCAGCCGTGACTG                                                                  >  SRR4302533.1820139/1‑94 (MQ=60)

                             GGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGG                                                                 >  SRR4302533.4357590/1‑94 (MQ=60)

                             GGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGG                                                                 >  SRR4302533.4945922/1‑94 (MQ=60)

                             GGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGG                                                                 <  SRR4302533.591743/94‑1 (MQ=60)

                             GGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGG                                                                 <  SRR4302533.632834/94‑1 (MQ=60)

                             GGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGG                                                                 >  SRR4302533.3469678/1‑94 (MQ=60)

                             GGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGG                                                                 >  SRR4302533.3598246/1‑94 (MQ=60)

                               TCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGC                                                               <  SRR4302533.4997739/94‑1 (MQ=60)

                               TCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGC                                                               >  SRR4302533.3819527/1‑94 (MQ=60)

                                CACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCC                                                              >  SRR4302533.1795606/1‑94 (MQ=60)

                                 ACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCG                                                             <  SRR4302533.6224109/94‑1 (MQ=60)

                                  CGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGC                                                            <  SRR4302533.807031/94‑1 (MQ=60)

                                   GCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCT                                                           >  SRR4302533.1169229/1‑94 (MQ=60)

                                    CCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTT                                                          >  SRR4302533.2638622/1‑94 (MQ=60)

                                    CCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTT                                                          <  SRR4302533.4148279/94‑1 (MQ=60)

                                     CAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTT                                                         <  SRR4302533.3482970/94‑1 (MQ=60)

                                       ATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCC                                                       <  SRR4302533.6330549/94‑1 (MQ=60)

                                       ATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCC                                                       <  SRR4302533.2425738/94‑1 (MQ=60)

                                         AGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAG                                                     <  SRR4302533.639743/94‑1 (MQ=60)

                                         AGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAG                                                     <  SRR4302533.5917860/94‑1 (MQ=60)

                                         AGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAG                                                     >  SRR4302533.4741681/1‑94 (MQ=60)

                                         AGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAG                                                     <  SRR4302533.4048553/94‑1 (MQ=60)

                                          GCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGG                                                    <  SRR4302533.5534810/94‑1 (MQ=60)

                                           CAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGC                                                   <  SRR4302533.2149395/94‑1 (MQ=60)

                                            AACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTGCCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCC                                                  <  SRR4302533.2773339/94‑1 (MQ=60)

                                            AACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCC                                                  >  SRR4302533.5686323/1‑94 (MQ=60)

                                            AACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCC                                                  >  SRR4302533.4540993/1‑94 (MQ=60)

                                            AACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCC                                                  <  SRR4302533.5623335/94‑1 (MQ=60)

                                            AACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCC                                                  >  SRR4302533.5740074/1‑94 (MQ=60)

                                            AACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCC                                                  <  SRR4302533.341482/94‑1 (MQ=60)

                                               GTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGC                                               >  SRR4302533.201567/1‑94 (MQ=60)

                                                TTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCG                                              <  SRR4302533.2585477/94‑1 (MQ=60)

                                                 TTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGC                                             <  SRR4302533.783487/94‑1 (MQ=60)

                                                  TTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCG                                            <  SRR4302533.6039453/94‑1 (MQ=60)

                                                     CAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGC                                         <  SRR4302533.4493701/94‑1 (MQ=60)

                                                       GCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTC                                       <  SRR4302533.1449266/94‑1 (MQ=60)

                                                       GCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTC                                       <  SRR4302533.3207702/94‑1 (MQ=60)

                                                       GCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTC                                       <  SRR4302533.3431680/94‑1 (MQ=60)

                                                        CGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCG                                      >  SRR4302533.5759330/1‑94 (MQ=60)

                                                         GCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGC                                     <  SRR4302533.3205778/94‑1 (MQ=60)

                                                         GCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGC                                     >  SRR4302533.5769177/1‑94 (MQ=60)

                                                          CGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCG                                    >  SRR4302533.955574/1‑94 (MQ=60)

                                                            GGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCC                                  >  SRR4302533.4460268/1‑94 (MQ=60)

                                                            GGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCC                                  <  SRR4302533.2016696/94‑1 (MQ=60)

                                                             GTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCC                                 >  SRR4302533.3810050/1‑94 (MQ=60)

                                                               TCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGC                               >  SRR4302533.3903522/1‑94 (MQ=60)

                                                               TCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGC                               >  SRR4302533.5931404/1‑94 (MQ=60)

                                                                CCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGTCCAGCGCGTGCTCGCGCCCGCC                              <  SRR4302533.3051966/94‑1 (MQ=60)

                                                                CCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCC                              <  SRR4302533.3259245/94‑1 (MQ=60)

                                                                CCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCC                              >  SRR4302533.1067262/1‑94 (MQ=60)

                                                                 CAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCG                             >  SRR4302533.4071344/1‑94 (MQ=60)

                                                                  AGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGT                            >  SRR4302533.1790563/1‑94 (MQ=60)

                                                                   GTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTT                           <  SRR4302533.419968/94‑1 (MQ=60)

                                                                   GTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTT                           <  SRR4302533.4232899/94‑1 (MQ=60)

                                                                   GTGCAGTGCCTGCATTACCCGGAGCAACAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTT                           <  SRR4302533.4984728/94‑1 (MQ=60)

                                                                    TGCAGTGCCTGCATTACCCGGAGCAGCAAAACCAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTA                          <  SRR4302533.6268402/94‑1 (MQ=60)

                                                                     GCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTAC                         <  SRR4302533.2780650/94‑1 (MQ=60)

                                                                       AGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCA                       <  SRR4302533.4738730/94‑1 (MQ=60)

                                                                        GTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGCTACCAA                      >  SRR4302533.2364306/1‑94 (MQ=60)

                                                                         TGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAAT                     <  SRR4302533.983090/94‑1 (MQ=60)

                                                                         TGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAAT                     <  SRR4302533.4092381/94‑1 (MQ=60)

                                                                           CCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCA                   >  SRR4302533.5269220/1‑94 (MQ=60)

                                                                            CTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAC                  <  SRR4302533.931238/94‑1 (MQ=60)

                                                                            CTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAC                  >  SRR4302533.1264621/1‑94 (MQ=60)

                                                                             TGCATTACCCGGAGCAGCAAAAACAGTCCCAACCAGCGGCGACTGGGCCGCTTTCCAGCCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACT                 <  SRR4302533.694818/94‑1 (MQ=60)

                                                                              GCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTA                <  SRR4302533.4495910/94‑1 (MQ=60)

                                                                              GCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTA                >  SRR4302533.3271918/1‑94 (MQ=60)

                                                                               CATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAA               <  SRR4302533.481519/94‑1 (MQ=60)

                                                                               CATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAA               >  SRR4302533.1228848/1‑94 (MQ=60)

                                                                                ATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGTCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAAT              <  SRR4302533.503786/94‑1 (MQ=60)

                                                                                ATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAAT              >  SRR4302533.430286/1‑94 (MQ=60)

                                                                                ATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAAT              <  SRR4302533.2336711/94‑1 (MQ=60)

                                                                                 TTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGCCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATA             <  SRR4302533.1779550/94‑1 (MQ=60)

                                                                                  TCCCCGGACCACCAAAAACAGCCTCAACCAGCGGCGACTGGGCCGCTTCCCAGTCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATAC            <  SRR4302533.5865722/94‑1 (MQ=60)

                                                                                  TACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCCCTAATAC            >  SRR4302533.5672006/1‑94 (MQ=60)

                                                                                  TACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATAC            >  SRR4302533.2127716/1‑94 (MQ=60)

                                                                                    CCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTT          <  SRR4302533.4609203/94‑1 (MQ=60)

                                                                                    CCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTT          <  SRR4302533.3858706/94‑1 (MQ=60)

                                                                                    CCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTT          >  SRR4302533.4355545/1‑94 (MQ=60)

                                                                                      CGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTC        >  SRR4302533.1394516/1‑94 (MQ=60)

                                                                                       GGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCA       <  SRR4302533.3725982/94‑1 (MQ=60)

                                                                                       GGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCA       <  SRR4302533.666996/94‑1 (MQ=60)

                                                                                       GGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCA       <  SRR4302533.129885/94‑1 (MQ=60)

                                                                                         AGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATC     >  SRR4302533.157647/1‑94 (MQ=60)

                                                                                           CAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATCTA   >  SRR4302533.1012076/1‑94 (MQ=60)

                                                                                            AGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATCTAT  >  SRR4302533.6250362/1‑94 (MQ=60)

                                                                                            AGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATCTAT  <  SRR4302533.6164560/94‑1 (MQ=60)

TGTGCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAACAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATCTAT  >  NC_000913/4203328‑4203513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`