Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,216,494	A→G	Y107C (TAC→TGC)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,216,494	0	A	G	100.0%	763.8 / NA	238	Y107C (TAC→TGC)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (113/125); total (113/125)

Only 100 of 247 total aligned reads displayed.

CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  >  NC_000913/4216404‑4216583

cATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTTCGTGTGCt                                                                                         >  2:4990408/1‑93 (MQ=255)

  tttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGt                                                                                       >  2:4034037/1‑93 (MQ=255)

   ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTc                                                                                      <  1:1277452/93‑1 (MQ=255)

   ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTc                                                                                      >  1:2993763/1‑93 (MQ=255)

   ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTc                                                                                      <  1:3694742/93‑1 (MQ=255)

   ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTc                                                                                      <  2:1363698/93‑1 (MQ=255)

     ttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCgg                                                                                    >  1:675491/1‑93 (MQ=255)

     ttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCgg                                                                                    <  1:59981/93‑1 (MQ=255)

        ctacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTa                                                                                 <  2:1945206/93‑1 (MQ=255)

         tacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAt                                                                                <  2:5585506/93‑1 (MQ=255)

         tacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAt                                                                                <  1:3992932/93‑1 (MQ=255)

          acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAtt                                                                               <  1:4805598/93‑1 (MQ=255)

          acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAtt                                                                               >  1:78081/1‑93 (MQ=255)

          acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAtt                                                                               <  1:413043/93‑1 (MQ=255)

           cGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAttt                                                                              >  2:315480/1‑93 (MQ=255)

           cGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAttt                                                                              <  1:5003010/93‑1 (MQ=255)

            gtgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTg                                                                             >  1:4564310/1‑93 (MQ=255)

             tgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGa                                                                            >  1:1041712/1‑93 (MQ=255)

              gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGac                                                                           <  2:1012234/93‑1 (MQ=255)

               ttAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGacc                                                                          <  1:5464423/93‑1 (MQ=255)

                tAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTCTTTGacca                                                                         <  2:6157564/93‑1 (MQ=255)

                  aaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGaccacc                                                                       <  1:735681/93‑1 (MQ=255)

                  aaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGaccacc                                                                       >  1:423738/1‑93 (MQ=255)

                   agagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcg                                                                      >  1:5103820/1‑93 (MQ=255)

                    gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACcgc                                                                     >  2:3568550/1‑93 (MQ=255)

                          tACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCtt                                                               >  2:2888308/1‑93 (MQ=255)

                            cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAc                                                             <  2:1616323/93‑1 (MQ=255)

                            cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTGTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAc                                                             >  1:3613830/1‑93 (MQ=255)

                             ccccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACt                                                            <  2:100047/92‑1 (MQ=255)

                              cccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTc                                                           <  2:3478496/93‑1 (MQ=255)

                              cccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTc                                                           >  1:3329826/1‑93 (MQ=255)

                               ccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTcc                                                          <  1:11924/93‑1 (MQ=255)

                               ccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTcc                                                          <  2:5903544/93‑1 (MQ=255)

                                cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                         >  2:631458/1‑93 (MQ=255)

                                  gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                       >  1:2366280/1‑93 (MQ=255)

                                  gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                       >  2:2432702/1‑93 (MQ=255)

                                  gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                       >  1:3291471/1‑93 (MQ=255)

                                  gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                       >  2:5510705/1‑93 (MQ=255)

                                  gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                       >  1:4427457/1‑93 (MQ=255)

                                  gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                       <  2:3343570/93‑1 (MQ=255)

                                   cTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAg                                                      <  2:3601869/93‑1 (MQ=255)

                                    tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                     >  2:3849548/1‑93 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                    >  2:3741864/1‑93 (MQ=255)

                                     gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                    <  1:5884860/93‑1 (MQ=255)

                                       tGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGc                                                  <  2:2604459/93‑1 (MQ=255)

                                        gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCAGAGCGGCt                                                 <  1:2831476/93‑1 (MQ=255)

                                           ggATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtttt                                              >  2:1151007/1‑93 (MQ=255)

                                           ggATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtttt                                              >  2:2388743/1‑93 (MQ=255)

                                             aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                            <  2:5764513/93‑1 (MQ=255)

                                             aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                            <  2:4715193/93‑1 (MQ=255)

                                              ttACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAt                                           >  1:6031170/1‑93 (MQ=255)

                                              ttACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAt                                           >  2:4483210/1‑93 (MQ=255)

                                               tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                          <  2:4315699/93‑1 (MQ=255)

                                               tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                          <  1:1115817/93‑1 (MQ=255)

                                               tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                          <  1:1513706/93‑1 (MQ=255)

                                                tcccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCt                                         <  1:1904816/92‑1 (MQ=255)

                                                 cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                        <  1:813218/93‑1 (MQ=255)

                                                  ccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCttt                                       <  2:18181/93‑1 (MQ=255)

                                                   cgcgCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTa                                      >  2:895199/1‑93 (MQ=255)

                                                     ggcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGc                                    <  1:4479867/92‑1 (MQ=255)

                                                     cgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGc                                    <  2:4470449/93‑1 (MQ=255)

                                                      gcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGct                                   >  1:4919895/1‑93 (MQ=255)

                                                        ttCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                                 <  1:5448918/93‑1 (MQ=255)

                                                           gagaTTGCGGAGAGCTTTTTTAACTCAGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAg                              >  1:2222457/1‑93 (MQ=255)

                                                            agaTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGc                             <  1:585089/93‑1 (MQ=255)

                                                              aTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                           <  2:4840175/93‑1 (MQ=255)

                                                              aTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                           >  1:3137081/1‑93 (MQ=255)

                                                              aTTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                           >  1:4381913/1‑93 (MQ=255)

                                                               ttGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCag                          >  2:622794/1‑93 (MQ=255)

                                                                 gCGGAGAGCTTTTTTAACTCCCTGTGCTGTCGGTTATTTGACCGCCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCagag                        <  2:1907445/93‑1 (MQ=255)

                                                                  cGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgc                       <  2:207612/93‑1 (MQ=255)

                                                                  cGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgc                       >  2:5320343/1‑93 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      <  2:5020420/93‑1 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      >  2:931198/1‑93 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      >  1:4698008/1‑93 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      >  1:4297986/1‑93 (MQ=255)

                                                                   ggAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                      >  2:2697019/1‑93 (MQ=255)

                                                                    gagaGCTTTTTTATCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcgc                     <  2:6281088/93‑1 (MQ=255)

                                                                    gagaGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcgc                     >  2:3262123/1‑93 (MQ=255)

                                                                    gagaGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcgc                     >  1:1663382/1‑93 (MQ=255)

                                                                      gagCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccg                   >  1:2908156/1‑93 (MQ=255)

                                                                      gagCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccg                   >  1:3502058/1‑93 (MQ=255)

                                                                        gCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCt                 <  2:3799218/93‑1 (MQ=255)

                                                                        gCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCt                 >  1:525575/1‑93 (MQ=255)

                                                                         cTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCtt                <  2:1723847/93‑1 (MQ=255)

                                                                          ttttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt               >  2:4370151/1‑93 (MQ=255)

                                                                          ttttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt               <  1:6127388/93‑1 (MQ=255)

                                                                          ttttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt               >  1:5567059/1‑93 (MQ=255)

                                                                           tttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTc              <  1:2719858/93‑1 (MQ=255)

                                                                             tttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGt            >  2:2766516/1‑93 (MQ=255)

                                                                                 aCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa        <  2:4869273/93‑1 (MQ=255)

                                                                                  cTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAt       <  2:2146672/93‑1 (MQ=255)

                                                                                  cTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAt       >  1:3461823/1‑93 (MQ=255)

                                                                                   tCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTCCTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt      <  1:1812269/93‑1 (MQ=255)

                                                                                   tCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt      >  1:654586/1‑93 (MQ=255)

                                                                                    ccGTGTGCTGTCGGTTATTTGACCACTGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTc     <  2:5089592/93‑1 (MQ=255)

                                                                                     cGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTcc    >  1:6103842/1‑93 (MQ=255)

                                                                                      gtgtGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTccc   >  2:2999232/1‑93 (MQ=255)

                                                                                      gtgtGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTccc   >  2:1113964/1‑93 (MQ=255)

                                                                                       tgtgCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTcccc  >  1:1011493/1‑93 (MQ=255)

CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  >  NC_000913/4216404‑4216583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCC  >  NC_000913/4216401‑4216587

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTG                                                                                               <  SRR4302533.2592856/94‑1 (MQ=60)

 GGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGC                                                                                              >  SRR4302533.3917290/1‑94 (MQ=60)

 GGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGC                                                                                              >  SRR4302533.6195483/1‑94 (MQ=60)

   CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTG                                                                                            <  SRR4302533.5688424/94‑1 (MQ=60)

   CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTG                                                                                            >  SRR4302533.554881/1‑94 (MQ=60)

     TTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTC                                                                                          >  SRR4302533.3012067/1‑94 (MQ=60)

      TTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCG                                                                                         <  SRR4302533.3717435/94‑1 (MQ=60)

      TTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCG                                                                                         >  SRR4302533.444029/1‑94 (MQ=60)

      TTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCG                                                                                         <  SRR4302533.1285130/94‑1 (MQ=60)

       TTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGG                                                                                        <  SRR4302533.3328670/94‑1 (MQ=60)

       TTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGG                                                                                        >  SRR4302533.679482/1‑94 (MQ=60)

        TTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGT                                                                                       <  SRR4302533.60372/94‑1 (MQ=60)

        TTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGT                                                                                       <  SRR4302533.2242537/94‑1 (MQ=60)

          ACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTA                                                                                     >  SRR4302533.797441/1‑94 (MQ=60)

           CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTAT                                                                                    >  SRR4302533.2559699/1‑94 (MQ=60)

            TACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATT                                                                                   >  SRR4302533.78583/1‑94 (MQ=60)

            TACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATT                                                                                   <  SRR4302533.3859727/94‑1 (MQ=60)

            TACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATT                                                                                   <  SRR4302533.4017244/94‑1 (MQ=60)

             ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTT                                                                                  <  SRR4302533.4834695/94‑1 (MQ=60)

             ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTT                                                                                  <  SRR4302533.415524/94‑1 (MQ=60)

              CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTG                                                                                 <  SRR4302533.3517893/94‑1 (MQ=60)

              CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTG                                                                                 <  SRR4302533.5033333/94‑1 (MQ=60)

              CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTG                                                                                 >  SRR4302533.4591984/1‑94 (MQ=60)

               GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGA                                                                                >  SRR4302533.1553371/1‑94 (MQ=60)

               GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGA                                                                                >  SRR4302533.1047836/1‑94 (MQ=60)

               GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGA                                                                                <  SRR4302533.5644673/94‑1 (MQ=60)

               GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGA                                                                                >  SRR4302533.6283356/1‑94 (MQ=60)

                TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGAC                                                                               >  SRR4302533.446111/1‑94 (MQ=60)

                  TTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCA                                                                             <  SRR4302533.5497352/94‑1 (MQ=60)

                    AAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACC                                                                           >  SRR4302533.426273/1‑94 (MQ=60)

                    AAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACC                                                                           >  SRR4302533.5025742/1‑94 (MQ=60)

                     AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCG                                                                          <  SRR4302533.1408524/94‑1 (MQ=60)

                     AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCG                                                                          <  SRR4302533.1136868/94‑1 (MQ=60)

                     AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCG                                                                          <  SRR4302533.740008/94‑1 (MQ=60)

                     AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCG                                                                          >  SRR4302533.5134649/1‑94 (MQ=60)

                      AGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGC                                                                         >  SRR4302533.4943001/1‑94 (MQ=60)

                      AGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCATTTTTAACTCCTTGTGCTGTCGGTTATTTGACCACCGC                                                                         >  SRR4302533.2773650/1‑94 (MQ=60)

                       GAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCT                                                                        >  SRR4302533.3434575/1‑94 (MQ=60)

                           ATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCT                                                                    >  SRR4302533.264906/1‑94 (MQ=60)

                            TTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTT                                                                   <  SRR4302533.4376357/94‑1 (MQ=60)

                              ACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAC                                                                 <  SRR4302533.910164/94‑1 (MQ=60)

                              ACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTGTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTAC                                                                 >  SRR4302533.3636067/1‑94 (MQ=60)

                               CACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACT                                                                >  SRR4302533.6234379/1‑94 (MQ=60)

                               CACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACT                                                                <  SRR4302533.1116499/94‑1 (MQ=60)

                                ACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTC                                                               >  SRR4302533.3350408/1‑94 (MQ=60)

                                ACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTC                                                               <  SRR4302533.2859294/94‑1 (MQ=60)

                                ACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTC                                                               >  SRR4302533.4929312/1‑94 (MQ=60)

                                 CCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCC                                                              >  SRR4302533.5196442/1‑94 (MQ=60)

                                 CCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCC                                                              <  SRR4302533.3714244/94‑1 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             <  SRR4302533.12017/94‑1 (MQ=60)

                                  CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                             >  SRR4302533.673822/1‑94 (MQ=60)

                                   CGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCG                                                            >  SRR4302533.3087980/1‑94 (MQ=60)

                                   CGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCG                                                            >  SRR4302533.3476712/1‑94 (MQ=60)

                                   CGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCG                                                            >  SRR4302533.1142280/1‑94 (MQ=60)

                                    GGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGA                                                           >  SRR4302533.2380915/1‑94 (MQ=60)

                                    GGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGA                                                           >  SRR4302533.4454219/1‑94 (MQ=60)

                                    GGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGA                                                           >  SRR4302533.1841413/1‑94 (MQ=60)

                                    GGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGA                                                           >  SRR4302533.590641/1‑94 (MQ=60)

                                    GGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGA                                                           >  SRR4302533.3311787/1‑94 (MQ=60)

                                      CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGATCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                         <  SRR4302533.4051477/94‑1 (MQ=60)

                                      CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                         >  SRR4302533.2219121/1‑94 (MQ=60)

                                      CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTCACTCCCGAGC                                                         >  SRR4302533.4747218/1‑94 (MQ=60)

                                       TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                        >  SRR4302533.2159020/1‑94 (MQ=60)

                                        GTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGG                                                       <  SRR4302533.5920422/94‑1 (MQ=60)

                                         TTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGC                                                      <  SRR4302533.3097035/94‑1 (MQ=60)

                                          TGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCT                                                     <  SRR4302533.1752825/94‑1 (MQ=60)

                                           GCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCAGAGCGGCTT                                                    <  SRR4302533.2848898/94‑1 (MQ=60)

                                            CCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTT                                                   <  SRR4302533.4653762/94‑1 (MQ=60)

                                               GATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTA                                                <  SRR4302533.5200719/94‑1 (MQ=60)

                                                ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTGCTCCCGAGCGGCTTTTTAT                                               <  SRR4302533.2882765/94‑1 (MQ=60)

                                                ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAT                                               <  SRR4302533.6138089/94‑1 (MQ=60)

                                                ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAT                                               >  SRR4302533.6067720/1‑94 (MQ=60)

                                                  TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                             <  SRR4302533.1522828/94‑1 (MQ=60)

                                                  TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                             <  SRR4302533.3542491/94‑1 (MQ=60)

                                                  TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                             <  SRR4302533.1122438/94‑1 (MQ=60)

                                                   TCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTT                                            <  SRR4302533.1916438/94‑1 (MQ=60)

                                                    CCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                           <  SRR4302533.817978/94‑1 (MQ=60)

                                                    CCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                           <  SRR4302533.1689702/94‑1 (MQ=60)

                                                    CCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                           <  SRR4302533.4641276/94‑1 (MQ=60)

                                                     CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTA                                          >  SRR4302533.756134/1‑94 (MQ=60)

                                                     CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTA                                          <  SRR4302533.4565462/94‑1 (MQ=60)

                                                     CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTA                                          <  SRR4302533.3590801/94‑1 (MQ=60)

                                                      CGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAG                                         <  SRR4302533.1272603/94‑1 (MQ=60)

                                                        GGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                       <  SRR4302533.4506968/94‑1 (MQ=60)

                                                        CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                       >  SRR4302533.4949698/1‑94 (MQ=60)

                                                          CTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCT                                     <  SRR4302533.6019454/94‑1 (MQ=60)

                                                          CTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCT                                     >  SRR4302533.3181803/1‑94 (MQ=60)

                                                           TTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTC                                    <  SRR4302533.1024224/94‑1 (MQ=60)

                                                           TTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTC                                    <  SRR4302533.5481742/94‑1 (MQ=60)

                                                             CGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                                  >  SRR4302533.699262/1‑94 (MQ=60)

                                                             CGAGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                                  <  SRR4302533.6096315/94‑1 (MQ=60)

                                                             CGAGATTGCGGAGAGCTTTTTTAACTCAGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                                  >  SRR4302533.2236186/1‑94 (MQ=60)

                                                               AGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                                >  SRR4302533.4222063/1‑94 (MQ=60)

                                                               AGATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                                <  SRR4302533.588539/94‑1 (MQ=60)

                                                                GATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCTA                               >  SRR4302533.60646/1‑94 (MQ=60)

                                                                GATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCA                               >  SRR4302533.3156368/1‑94 (MQ=60)

                                                                GATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCA                               >  SRR4302533.4295898/1‑94 (MQ=60)

                                                                GATTGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCA                               >  SRR4302533.4408370/1‑94 (MQ=60)

                                                                   TGCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAG                            <  SRR4302533.4381238/94‑1 (MQ=60)

                                                                    GCGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGC                           <  SRR4302533.3815823/94‑1 (MQ=60)

                                                                     CGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCG                          >  SRR4302533.4323833/1‑94 (MQ=60)

                                                                     CGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCG                          >  SRR4302533.4726485/1‑94 (MQ=60)

                                                                     CGGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCG                          <  SRR4302533.6007127/94‑1 (MQ=60)

                                                                      GGAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGC                         >  SRR4302533.1673437/1‑94 (MQ=60)

                                                                       GAGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCC                        <  SRR4302533.6163329/94‑1 (MQ=60)

                                                                        AGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                       >  SRR4302533.3523653/1‑94 (MQ=60)

                                                                        AGAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                       >  SRR4302533.2926009/1‑94 (MQ=60)

                                                                         GAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGC                      <  SRR4302533.14045/94‑1 (MQ=60)

                                                                         GAGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCACGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGC                      >  SRR4302533.4475562/1‑94 (MQ=60)

                                                                          AGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTTCTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     <  SRR4302533.5036819/94‑1 (MQ=60)

                                                                          AGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     >  SRR4302533.528689/1‑94 (MQ=60)

                                                                          AGCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                     <  SRR4302533.2983916/94‑1 (MQ=60)

                                                                           GCTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTNNNTCTCAGCCAGANCNCNGNTT                    >  SRR4302533.3153600/1‑94 (MQ=60)

                                                                            CTTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTT                   >  SRR4302533.5600708/1‑94 (MQ=60)

                                                                             TTTTTTACCTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTC                  <  SRR4302533.5421815/94‑1 (MQ=60)

                                                                             TTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTC                  <  SRR4302533.548662/94‑1 (MQ=60)

                                                                             TTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTC                  <  SRR4302533.6164317/94‑1 (MQ=60)

                                                                             TTTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTC                  <  SRR4302533.731635/94‑1 (MQ=60)

                                                                              TTTTTAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCG                 <  SRR4302533.2736553/94‑1 (MQ=60)

                                                                                   AACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCA            >  SRR4302533.801436/1‑94 (MQ=60)

                                                                                    ACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAT           >  SRR4302533.3483190/1‑94 (MQ=60)

                                                                                    ACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAT           <  SRR4302533.5483984/94‑1 (MQ=60)

                                                                                     CTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT          >  SRR4302533.658451/1‑94 (MQ=60)

                                                                                      TCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTCCTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC         <  SRR4302533.1823339/94‑1 (MQ=60)

                                                                                      TCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC         <  SRR4302533.4159333/94‑1 (MQ=60)

                                                                                       CCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCC        >  SRR4302533.6140685/1‑94 (MQ=60)

                                                                                         GTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC      >  SRR4302533.1017412/1‑94 (MQ=60)

                                                                                            TGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCC   >  SRR4302533.2959847/1‑94 (MQ=60)

                                                                                            TGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCC   >  SRR4302533.210136/1‑94 (MQ=60)

                                                                                            TGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCC   <  SRR4302533.5695515/94‑1 (MQ=60)

                                                                                             GCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCC  <  SRR4302533.2575916/94‑1 (MQ=60)

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCC  >  NC_000913/4216401‑4216587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`