Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,249,909	+C	intergenic (+47/‑176)	yjbI → / → ubiC	hypothetical protein/chorismate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,249,905	1	.	C	99.6%	753.8 / ‑4.2	235	intergenic (+43/‑180)	yjbI/ubiC	hypothetical protein/chorismate lyase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (121/113); total (122/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 253 total aligned reads displayed.

TTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑CCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTT  >  NC_000913/4249813‑4249991

tttttACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATg                                                                                         >  1:239999/1‑93 (MQ=255)

 ttttACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑c                                                                                       >  1:49193/1‑93 (MQ=255)

  tttACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑cc                                                                                      >  2:5855598/1‑93 (MQ=255)

     aCTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑ccccc                                                                                   <  2:1969559/93‑2 (MQ=255)

     aCTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑ccccc                                                                                   >  2:170523/1‑92 (MQ=255)

          gAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACgg                                                                               <  2:2271303/93‑1 (MQ=255)

           aGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGt                                                                              >  1:6270445/1‑93 (MQ=255)

              tGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGc                                                                           <  1:3807327/93‑1 (MQ=255)

              tGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCAGCCTGGACGGTGATGCCCCCTACGGTTGc                                                                           <  1:3868686/93‑1 (MQ=255)

                  gAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTc                                                                       <  1:742130/93‑1 (MQ=255)

                  gAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTc                                                                       >  1:3487891/1‑93 (MQ=255)

                   aGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCg                                                                      >  1:3192697/1‑93 (MQ=255)

                    gCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGc                                                                     <  2:2474637/93‑1 (MQ=255)

                    gCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGc                                                                     >  1:5525578/1‑93 (MQ=255)

                     cTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGcc                                                                    >  2:5755704/1‑93 (MQ=255)

                       cATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAg                                                                  >  2:296103/1‑93 (MQ=255)

                       cATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGACGCCCCCTACGGTTGCCCTCGCCAg                                                                  <  2:6074265/93‑1 (MQ=255)

                        aTCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCCCCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGc                                                                 <  1:4488800/93‑1 (MQ=255)

                         tCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCa                                                                <  2:1039190/93‑1 (MQ=255)

                         tCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCa                                                                >  2:218147/1‑93 (MQ=255)

                         tCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCa                                                                <  1:3022632/93‑1 (MQ=255)

                         tCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTAGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCACGCCAGCa                                                                <  1:4064536/93‑1 (MQ=255)

                           gTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACg                                                              >  1:1935559/1‑93 (MQ=255)

                            tataATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACgg                                                             >  1:1988103/1‑93 (MQ=255)

                            tataATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACgg                                                             <  2:5623862/93‑1 (MQ=255)

                             ataATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACggg                                                            <  2:3351256/93‑1 (MQ=255)

                             ataATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACggg                                                            <  2:4548882/93‑1 (MQ=255)

                              taATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGc                                                           >  2:4218452/1‑93 (MQ=255)

                                 tGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATc                                                        >  2:4965150/1‑93 (MQ=255)

                                  gAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCg                                                       <  2:4635379/93‑1 (MQ=255)

                                    ttAAGGGTATTTTAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTCCGGTTGCCCTCGCCAGCACGGGCATCGGt                                                     <  2:6300124/92‑1 (MQ=255)

                                     tAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTa                                                    >  1:5901960/1‑93 (MQ=255)

                                        gggTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAg                                                 <  1:131299/93‑1 (MQ=255)

                                        gggTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAg                                                 >  1:5371728/1‑93 (MQ=255)

                                        gggTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAg                                                 >  1:1787591/1‑93 (MQ=255)

                                        gggTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAg                                                 <  2:5990155/93‑1 (MQ=255)

                                        gggTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAg                                                 >  1:3263093/1‑93 (MQ=255)

                                            gtTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTa                                             >  2:5633064/1‑93 (MQ=255)

                                             tttAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTaa                                            >  1:5057067/1‑93 (MQ=255)

                                              ttAAGTAAAGGAAAACATCCCCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAg                                           <  1:1526050/93‑1 (MQ=255)

                                              ttAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAg                                           <  2:3778449/93‑1 (MQ=255)

                                              ttAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAg                                           >  2:3253388/1‑93 (MQ=255)

                                                 aGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGtt                                        >  1:4328485/1‑93 (MQ=255)

                                                 aGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGtt                                        <  2:3313909/93‑1 (MQ=255)

                                                 aGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGtt                                        <  1:776521/93‑1 (MQ=255)

                                                    aaaGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTCCGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCaa                                     <  1:4087810/93‑1 (MQ=255)

                                                    aaaGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCaa                                     <  1:5623706/93‑1 (MQ=255)

                                                    aaaGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCaa                                     >  2:4264795/1‑93 (MQ=255)

                                                    aaaGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCaa                                     >  1:4681762/1‑93 (MQ=255)

                                                      aGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACa                                   <  1:419148/93‑1 (MQ=255)

                                                       ggAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACAt                                  <  1:1023694/93‑1 (MQ=255)

                                                        gAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCaagctc                                 >  2:2105643/1‑89 (MQ=255)

                                                        gAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATc                                 <  1:3974349/93‑1 (MQ=255)

                                                        gAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATc                                 <  2:260280/93‑1 (MQ=255)

                                                         aaaaCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCg                                >  2:4902787/1‑93 (MQ=255)

                                                         aaaaCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCg                                >  2:5440776/1‑93 (MQ=255)

                                                          aaaCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGt                               >  2:1444784/1‑93 (MQ=255)

                                                          aaaCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGt                               <  2:1838063/93‑1 (MQ=255)

                                                          aaaCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGt                               >  2:3914775/1‑93 (MQ=255)

                                                           aaCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGtt                              >  2:3296386/1‑93 (MQ=255)

                                                           aaCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGtt                              >  1:2886828/1‑93 (MQ=255)

                                                            aCATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGttt                             >  1:1196821/1‑93 (MQ=255)

                                                             cATCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTCCAACATCGtgtt                            >  1:1872621/1‑93 (MQ=255)

                                                               tCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTAc                          >  2:3507557/1‑93 (MQ=255)

                                                               tCACCGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTAc                          >  2:3305627/1‑93 (MQ=255)

                                                                  ccGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCAc                       >  2:3166665/1‑93 (MQ=255)

                                                                   cGTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGCTCAACATCGTTTTACCACt                      >  2:1074598/1‑93 (MQ=255)

                                                                   cGTTCCTGGCATCCTGGACGGAGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGGTCGACATCGTTTTAcgagt                      >  1:1306981/1‑89 (MQ=255)

                                                                    gTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACtt                     >  1:5273880/1‑93 (MQ=255)

                                                                    gTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACtt                     >  1:3700036/1‑93 (MQ=255)

                                                                    gTTCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACtt                     <  1:3465627/93‑1 (MQ=255)

                                                                     ttCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTc                    >  2:3025546/1‑93 (MQ=255)

                                                                     ttCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTc                    >  1:559570/1‑93 (MQ=255)

                                                                      tCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCa                   <  1:66872/93‑1 (MQ=255)

                                                                      tCCTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCa                   <  2:5372002/93‑1 (MQ=255)

                                                                        cTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATg                 >  2:5251714/1‑93 (MQ=255)

                                                                        cTGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGCCATCGGTAAAGCGTAAGGTTCAACATCGGTTTACCACTTCAtc                 >  1:3039612/1‑92 (MQ=255)

                                                                         tGGCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGc                >  2:5144962/1‑93 (MQ=255)

                                                                         tGGCAACCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGc                >  2:4107342/1‑93 (MQ=255)

                                                                         cggCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGc                <  1:5856945/92‑1 (MQ=255)

                                                                          ggCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCg               <  2:3089815/93‑1 (MQ=255)

                                                                           gCATCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGa              >  2:2814485/1‑93 (MQ=255)

                                                                             aTCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAtt            >  2:2124536/1‑93 (MQ=255)

                                                                             aTCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAtt            >  2:1543875/1‑93 (MQ=255)

                                                                             aTCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAtt            >  2:3416646/1‑93 (MQ=255)

                                                                              tCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttg           >  2:5774231/1‑93 (MQ=255)

                                                                              tCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttg           >  1:1042600/1‑93 (MQ=255)

                                                                              tCCTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttg           >  1:217270/1‑93 (MQ=255)

                                                                               ccTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttgt          <  2:2917137/93‑1 (MQ=255)

                                                                               ccTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttgt          <  1:252684/93‑1 (MQ=255)

                                                                                cTGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttgtt         >  1:2105682/1‑93 (MQ=255)

                                                                                 tGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttgttg        >  2:3525464/1‑93 (MQ=255)

                                                                                 tGGACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAttgttg        <  2:2537987/93‑1 (MQ=255)

                                                                                  ggACGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGc       <  2:4478054/93‑1 (MQ=255)

                                                                                    aCGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGt     <  1:5123845/93‑1 (MQ=255)

                                                                                    aCGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGt     <  1:2722645/93‑1 (MQ=255)

                                                                                    aCGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGt     <  2:850767/93‑1 (MQ=255)

                                                                                     cGGTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGtt    <  1:1638238/93‑1 (MQ=255)

                                                                                       gTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGtttt  >  2:4061786/1‑93 (MQ=255)

                                                                                       gTGATGCCCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGtttt  <  1:4831419/93‑1 (MQ=255)

TTTTTACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑CCCCTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTT  >  NC_000913/4249813‑4249991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCGTATT  >  NC_000913/4249817‑4250002

TACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑CCC‑CC                                                                                              >  SRR4302533.1824257/1‑94 (MQ=60)

  CTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑CCC‑Ccta                                                                                            >  SRR4302533.1035454/1‑91 (MQ=60)

   TGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTAC                                                                                            <  SRR4302533.1318433/94‑1 (MQ=60)

    GCGAGTTGGCGAGCTCATCGTATAATGAGTAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACG                                                                                           <  SRR4302533.2916686/94‑1 (MQ=60)

      GAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGT                                                                                         >  SRR4302533.6307905/1‑94 (MQ=60)

       AGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGTCATCCTGGACGGTGATG‑CCC‑Ccttcggtt                                                                                       <  SRR4302533.3206562/94‑9 (MQ=60)

       AGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTT                                                                                        <  SRR4302533.283082/94‑1 (MQ=60)

         TTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGC                                                                                      >  SRR4302533.5350254/1‑94 (MQ=60)

          TGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCC                                                                                     >  SRR4302533.5550583/1‑94 (MQ=60)

          TGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCC                                                                                     <  SRR4302533.3830619/94‑1 (MQ=60)

          TGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCAGCCTGGACGGTGATGCCCC‑CTACGGTTGCC                                                                                     <  SRR4302533.3892305/94‑1 (MQ=60)

           GGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCC                                                                                    >  SRR4302533.5354673/1‑94 (MQ=60)

             CGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTC                                                                                  >  SRR4302533.3509396/1‑94 (MQ=60)

              GAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCG                                                                                 <  SRR4302533.746499/94‑1 (MQ=60)

              GAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCG                                                                                 >  SRR4302533.3212336/1‑94 (MQ=60)

               AGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGC                                                                                >  SRR4302533.5558936/1‑94 (MQ=60)

                GCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCC                                                                               >  SRR4302533.1052588/1‑94 (MQ=60)

                  TCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAG                                                                             >  SRR4302533.5579656/1‑94 (MQ=60)

                    ATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCCCCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCA                                                                           <  SRR4302533.4515972/94‑1 (MQ=60)

                     TCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCAC                                                                          <  SRR4302533.3041125/94‑1 (MQ=60)

                     TCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTAGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCACGCCAGCAC                                                                          <  SRR4302533.4089276/94‑1 (MQ=60)

                      CGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACG                                                                         >  SRR4302533.4089861/1‑94 (MQ=60)

                      CGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACG                                                                         <  SRR4302533.482314/94‑1 (MQ=60)

                      CGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACG                                                                         >  SRR4302533.1947366/1‑94 (MQ=60)

                       GTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGG                                                                        >  SRR4302533.4053512/1‑94 (MQ=60)

                       GTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGG                                                                        >  SRR4302533.3628099/1‑94 (MQ=60)

                       GTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGG                                                                        >  SRR4302533.2000264/1‑94 (MQ=60)

                        TATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑CCCACTACGGTTGCCCTCGCCAGCACGGG                                                                       >  SRR4302533.1551297/1‑94 (MQ=60)

                         ATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGC                                                                      >  SRR4302533.2768568/1‑94 (MQ=60)

                         ATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGC                                                                      >  SRR4302533.4207485/1‑94 (MQ=60)

                          TAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCA                                                                     <  SRR4302533.3506461/94‑1 (MQ=60)

                           AATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCAT                                                                    <  SRR4302533.200884/94‑1 (MQ=60)

                            ATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATC                                                                   <  SRR4302533.2337227/94‑1 (MQ=60)

                             TGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCG                                                                  <  SRR4302533.267918/94‑1 (MQ=60)

                             TGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCG                                                                  <  SRR4302533.5993880/94‑1 (MQ=60)

                              TAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGG                                                                 <  SRR4302533.5070142/94‑1 (MQ=60)

                                ATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTA                                                               >  SRR4302533.643857/1‑94 (MQ=60)

                                ATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTA                                                               >  SRR4302533.5937601/1‑94 (MQ=60)

                                   AGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAG                                                            >  SRR4302533.5404002/1‑94 (MQ=60)

                                   AGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAG                                                            <  SRR4302533.5708158/94‑1 (MQ=60)

                                   AGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAG                                                            >  SRR4302533.1798454/1‑94 (MQ=60)

                                   AGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAG                                                            >  SRR4302533.3283223/1‑94 (MQ=60)

                                   AGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTG‑CCTCGCCAGCACGGGCATCGGTAAAGC                                                           <  SRR4302533.1972065/94‑1 (MQ=60)

                                    GGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGC                                                           <  SRR4302533.132123/94‑1 (MQ=60)

                                    GGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGC                                                           <  SRR4302533.3673921/94‑1 (MQ=60)

                                        GTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAA                                                       >  SRR4302533.5087631/1‑94 (MQ=60)

                                         TTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAG                                                      <  SRR4302533.1591535/94‑1 (MQ=60)

                                         TTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAG                                                      <  SRR4302533.3470708/94‑1 (MQ=60)

                                          TTAAGTAAAGGAAAACATCCCCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGG                                                     <  SRR4302533.1535234/94‑1 (MQ=60)

                                          TTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGG                                                     >  SRR4302533.1837420/1‑94 (MQ=60)

                                           TAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGT                                                    >  SRR4302533.5092139/1‑94 (MQ=60)

                                           TAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGT                                                    <  SRR4302533.735929/94‑1 (MQ=60)

                                            AAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTT                                                   >  SRR4302533.4354563/1‑94 (MQ=60)

                                            AAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTT                                                   <  SRR4302533.4075398/94‑1 (MQ=60)

                                             AGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTC                                                  >  SRR4302533.2472325/1‑94 (MQ=60)

                                             AGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTC                                                  <  SRR4302533.781085/94‑1 (MQ=60)

                                               TAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAA                                                <  SRR4302533.3884206/94‑1 (MQ=60)

                                               TAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAA                                                >  SRR4302533.4710131/1‑94 (MQ=60)

                                                AAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTCCGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAAC                                               <  SRR4302533.4112695/94‑1 (MQ=60)

                                                AAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAAC                                               <  SRR4302533.5657698/94‑1 (MQ=60)

                                                 AAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACA                                              >  SRR4302533.3859205/1‑94 (MQ=60)

                                                 AAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACA                                              >  SRR4302533.2447678/1‑94 (MQ=60)

                                                 AAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTACCCTCGCCAGCACGGTCATCGGGAAAGCGTAAGGGTCAACA                                              >  SRR4302533.3039832/1‑94 (MQ=60)

                                                  AGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACAT                                             <  SRR4302533.421659/94‑1 (MQ=60)

                                                  AGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACAT                                             >  SRR4302533.1985719/1‑94 (MQ=60)

                                                  AGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACAT                                             <  SRR4302533.3128/94‑1 (MQ=60)

                                                   GGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATC                                            <  SRR4302533.1029711/94‑1 (MQ=60)

                                                   GGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATC                                            >  SRR4302533.3123559/1‑94 (MQ=60)

                                                    GAAAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCG                                           <  SRR4302533.3998548/94‑1 (MQ=60)

                                                      AAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTT                                         >  SRR4302533.5657474/1‑94 (MQ=60)

                                                      AAACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTT                                         >  SRR4302533.2904579/1‑94 (MQ=60)

                                                       AACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTT                                        <  SRR4302533.6222041/94‑1 (MQ=60)

                                                       AACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTT                                        <  SRR4302533.2156131/94‑1 (MQ=60)

                                                       AACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTT                                        >  SRR4302533.1204011/1‑94 (MQ=60)

                                                        ACATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTCCAACATCGTGTT                                       >  SRR4302533.1884037/1‑94 (MQ=60)

                                                          ATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTAC                                     >  SRR4302533.3949883/1‑94 (MQ=60)

                                                          ATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTAC                                     >  SRR4302533.3318341/1‑94 (MQ=60)

                                                          ATCACCGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTAC                                     >  SRR4302533.4330027/1‑94 (MQ=60)

                                                              CCGTTCCTGGCATCCTGGACGGAGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGGTCGACATCGTTTTACgagt                                 >  SRR4302533.1314830/1‑90 (MQ=60)

                                                               CGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTT                                >  SRR4302533.5305500/1‑94 (MQ=60)

                                                               CGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTT                                <  SRR4302533.5921284/94‑1 (MQ=60)

                                                               CGTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTT                                >  SRR4302533.3722762/1‑94 (MQ=60)

                                                                GTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTC                               >  SRR4302533.562893/1‑94 (MQ=60)

                                                                GTTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTC                               <  SRR4302533.3487017/94‑1 (MQ=60)

                                                                 TTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCA                              >  SRR4302533.4899050/1‑94 (MQ=60)

                                                                 TTCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGAACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCA                              <  SRR4302533.4453677/94‑1 (MQ=60)

                                                                  TCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCAT                             >  SRR4302533.4416185/1‑94 (MQ=60)

                                                                  TCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCAT                             <  SRR4302533.1318318/94‑1 (MQ=60)

                                                                  TCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCAT                             <  SRR4302533.5243906/94‑1 (MQ=60)

                                                                  TCCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCAT                             <  SRR4302533.67307/94‑1 (MQ=60)

                                                                   CCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATG                            <  SRR4302533.4613490/94‑1 (MQ=60)

                                                                   CCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATG                            >  SRR4302533.3764449/1‑94 (MQ=60)

                                                                   CCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATG                            >  SRR4302533.3058042/1‑94 (MQ=60)

                                                                   CCTGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGCCATCGGTAAAGCGTAAGGTTCAACATCGGTTTACCACTTCATC                            >  SRR4302533.3058214/1‑94 (MQ=60)

                                                                     TGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCG                          >  SRR4302533.1896700/1‑94 (MQ=60)

                                                                     TGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCG                          >  SRR4302533.2155297/1‑94 (MQ=60)

                                                                     CGGCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCG                          <  SRR4302533.5892351/94‑1 (MQ=60)

                                                                       GCATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGAT                        >  SRR4302533.3267072/1‑94 (MQ=60)

                                                                        CATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATT                       <  SRR4302533.5712546/94‑1 (MQ=60)

                                                                        CATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATT                       >  SRR4302533.1776825/1‑94 (MQ=60)

                                                                        CATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATT                       >  SRR4302533.4121715/1‑94 (MQ=60)

                                                                         ATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTG                      >  SRR4302533.218632/1‑94 (MQ=60)

                                                                         ATCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTG                      >  SRR4302533.1048730/1‑94 (MQ=60)

                                                                          TCCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGT                     <  SRR4302533.1411991/94‑1 (MQ=60)

                                                                           CCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTT                    <  SRR4302533.254237/94‑1 (MQ=60)

                                                                           CCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTT                    >  SRR4302533.69135/1‑94 (MQ=60)

                                                                           CCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTT                    >  SRR4302533.2118667/1‑94 (MQ=60)

                                                                           CCTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTT                    <  SRR4302533.746560/94‑1 (MQ=60)

                                                                            CTGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTG                   >  SRR4302533.5089958/1‑94 (MQ=60)

                                                                             TGGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGC                  >  SRR4302533.5603650/1‑94 (MQ=60)

                                                                              GGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCG                 <  SRR4302533.785070/94‑1 (MQ=60)

                                                                              GGACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCG                 >  SRR4302533.488048/1‑94 (MQ=60)

                                                                                ACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTT               <  SRR4302533.2739354/94‑1 (MQ=60)

                                                                                ACGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTT               <  SRR4302533.5154800/94‑1 (MQ=60)

                                                                                 CGGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTT              <  SRR4302533.1648157/94‑1 (MQ=60)

                                                                                  GGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTT             >  SRR4302533.4795333/1‑94 (MQ=60)

                                                                                  GGTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTT             <  SRR4302533.1313920/94‑1 (MQ=60)

                                                                                   GTGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTT            <  SRR4302533.4860701/94‑1 (MQ=60)

                                                                                    TGATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTG           >  SRR4302533.1503152/1‑94 (MQ=60)

                                                                                      ATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTT         <  SRR4302533.5472486/94‑1 (MQ=60)

                                                                                      ATGCCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTT         <  SRR4302533.4513621/94‑1 (MQ=60)

                                                                                       tgcCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTG        >  SRR4302533.5626513/4‑94 (MQ=60)

                                                                                       tgcCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTG        <  SRR4302533.3527242/91‑1 (MQ=60)

                                                                                       tgcCCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTCTACCACTTCATGCGATTGTTGCGTTTTTGTTG        <  SRR4302533.1135665/91‑1 (MQ=60)

                                                                                        C‑CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCG      <  SRR4302533.5827181/94‑1 (MQ=60)

                                                                                        C‑CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCG      >  SRR4302533.5430457/1‑94 (MQ=60)

                                                                                        C‑CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCG      <  SRR4302533.668630/94‑1 (MQ=60)

                                                                                        C‑CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCG      >  SRR4302533.5043113/1‑94 (MQ=60)

                                                                                        C‑CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCG      >  SRR4302533.4930978/1‑94 (MQ=60)

                                                                                          CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCGT     <  SRR4302533.5977865/94‑1 (MQ=60)

                                                                                          CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCGT     >  SRR4302533.492463/1‑94 (MQ=60)

                                                                                          CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCGT     >  SRR4302533.3045382/1‑94 (MQ=60)

                                                                                              CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCGTATT  <  SRR4302533.2038632/94‑1 (MQ=60)

                                                                                              CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCGTATT  >  SRR4302533.920533/1‑94 (MQ=60)

TACTGCGAGTTGGCGAGCTCATCGTATAATGAATAAGGGTGTTTAAGTAAAGGAAAACATCACCGTTCCTGGCATCCTGGACGGTGATG‑CCC‑CTACGGTTGCCCTCGCCAGCACGGGCATCGGTAAAGCGTAAGGTTCAACATCGTTTTACCACTTCATGCGATTGTTGCGTTTTTGTTGCGTATT  >  NC_000913/4249817‑4250002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`