Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,251,643 A→G S801S (AGT→AGC)  plsB ← glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,251,6430AG100.0% 685.0 / NA 214S801S (AGT→AGC) plsBglycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (126/88);  total (126/88)

Only 100 of 224 total aligned reads displayed.

ATTTATTGAATTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCT  >  NC_000913/4251553‑4251732
                                                                                           |                                                                                         
ttttATGAAATTTGCGT‑ATTAGGGAAGGCCGGATAAGGCGTTTCCGCCGCAGCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGct                                                                                         <  1:394218/92‑1 (MQ=25)
aTTTATTAAATTGGCAG‑TTTATGGAAGGCCGAATAAGCCGTTTTCCCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGct                                                                                         <  1:2667265/93‑1 (MQ=37)
 tttATTGAATTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGctc                                                                                        >  1:5378022/1‑93 (MQ=255)
  ttattaATTTGGCAG‑ATTATGGAGGGCGGGATAGGGCGTTTCCGCCGCACCCGCCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGctct                                                                                       <  2:1469778/93‑1 (MQ=255)
  ttATTGAATTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGctct                                                                                       >  2:5746626/1‑93 (MQ=255)
    tatGAATTTGGAGAATTAGGGAGGGCGGAATAGGGGTTTTCCCCCCCACCGGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCtctc                                                                                      <  1:1175689/91‑1 (MQ=255)
    aTTGAATTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCa                                                                                     >  1:1751143/1‑93 (MQ=255)
    aTTGAATTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCa                                                                                     >  2:5622496/1‑93 (MQ=255)
     ttGAATTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCaa                                                                                    >  2:2724983/1‑93 (MQ=255)
        aaTTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCg                                                                                 >  2:3490354/1‑93 (MQ=255)
          tttGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTc                                                                               >  2:675730/1‑93 (MQ=255)
          tttGCAG‑ATTAGGGAGGGCGGAATAAGGCGTTTTCCCCCCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTc                                                                               <  2:774945/93‑1 (MQ=37)
             gCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCaaa                                                                            >  2:4401096/1‑93 (MQ=255)
               gataTTAGGGAAGGCGGGATAAGGCGTTTCCGCCGCATCCGCCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAAc                                                                           <  2:106725/90‑1 (MQ=37)
                g‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGc                                                                         >  1:2031476/1‑93 (MQ=255)
                ataTTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACg                                                                          <  2:1614270/91‑1 (MQ=255)
                  aTTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCa                                                                        <  1:852567/93‑1 (MQ=255)
                   ttATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCAc                                                                       >  2:1279448/1‑93 (MQ=255)
                   ttATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCAc                                                                       >  2:3779111/1‑93 (MQ=255)
                    tATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACg                                                                      >  1:2345425/1‑93 (MQ=255)
                      tgaaaGGCCGGATAAGGCGTTTTCGCCGCATCGGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTc                                                                    <  2:1865298/90‑1 (MQ=38)
                      tGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTc                                                                    >  2:5463105/1‑93 (MQ=255)
                        gAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTg                                                                  >  2:841931/1‑93 (MQ=255)
                            gCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGt                                                              >  1:4303303/1‑93 (MQ=255)
                             ccGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTa                                                             >  1:4962091/1‑93 (MQ=255)
                                gATAGGGCTTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATc                                                          <  1:327941/93‑1 (MQ=39)
                                gATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATc                                                          <  1:1952256/93‑1 (MQ=255)
                                gATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATc                                                          >  1:5199542/1‑93 (MQ=255)
                                gATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATc                                                          >  2:1053339/1‑93 (MQ=255)
                                gATAAGGCGTTTTCGCCCCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATc                                                          <  1:976980/93‑1 (MQ=255)
                                gATAAGACGTATTCGCGGCCTCCGACAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATc                                                          <  1:615969/93‑1 (MQ=255)
                                  tagggCTTTTTCGCCGCACCGGCCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCaa                                                        <  1:1317470/90‑1 (MQ=255)
                                    aGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACt                                                      >  2:6203046/1‑93 (MQ=255)
                                    aGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACt                                                      >  1:5024235/1‑93 (MQ=255)
                                    aGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACt                                                      >  2:4810611/1‑93 (MQ=255)
                                      gctttttCCCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTcc                                                    <  2:4976882/90‑1 (MQ=255)
                                      gCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTcc                                                    >  2:6098852/1‑93 (MQ=255)
                                         ttttCGCTGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGcc                                                 >  1:4684754/1‑93 (MQ=255)
                                         ttttCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGcc                                                 >  1:668203/1‑93 (MQ=255)
                                          tttCGCCGCACCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCa                                                <  1:5638739/93‑1 (MQ=255)
                                              gccgcATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCaa                                            >  1:4056104/1‑93 (MQ=255)
                                               ccccATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAAc                                           <  1:3457526/90‑1 (MQ=255)
                                                cgcATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACt                                          >  1:791819/1‑93 (MQ=255)
                                                 gcATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTg                                         >  2:1539479/1‑93 (MQ=255)
                                                   aTCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGAt                                       >  2:2042573/1‑93 (MQ=255)
                                                       ggCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAAc                                   >  2:312303/1‑93 (MQ=255)
                                                        gCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAAcc                                  <  2:416746/93‑1 (MQ=255)
                                                        gCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAAcc                                  >  1:5291827/1‑93 (MQ=255)
                                                        gCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAAcc                                  >  2:3087121/1‑93 (MQ=255)
                                                         cAATTCTCTCTGATTCCCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAACCGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCt                                 <  2:5665835/93‑1 (MQ=255)
                                                         cAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCt                                 <  1:574495/93‑1 (MQ=255)
                                                          aaTTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCtt                                >  2:2161488/1‑93 (MQ=255)
                                                           aTTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTc                               >  1:2177231/1‑93 (MQ=255)
                                                            ttCTCTCTTATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAGCGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAATCCTTCa                              <  2:261307/93‑1 (MQ=255)
                                                            ttCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCa                              >  1:1886539/1‑93 (MQ=255)
                                                            ttCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCa                              <  1:5541389/93‑1 (MQ=255)
                                                             tctctcTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAt                             >  1:1741659/1‑93 (MQ=255)
                                                             tctctcTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAt                             >  1:6057119/1‑93 (MQ=255)
                                                             tctctcTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAt                             >  1:3018082/1‑93 (MQ=255)
                                                              ctctctGATTCCCCTTCGCCCTGCGTCGCGCGCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATc                            <  1:4740174/93‑1 (MQ=255)
                                                              ctctctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATc                            <  2:4837820/93‑1 (MQ=255)
                                                              ctctctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATc                            <  1:2807019/93‑1 (MQ=255)
                                                               tctctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCg                           >  1:2448726/1‑93 (MQ=255)
                                                               tctctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCg                           >  2:5124169/1‑93 (MQ=255)
                                                                 tctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGtt                         >  2:1516245/1‑93 (MQ=255)
                                                                 tctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGtt                         >  1:3732934/1‑93 (MQ=255)
                                                                  ctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGttt                        <  2:5260179/93‑1 (MQ=255)
                                                                  ctGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGttt                        <  2:1924406/93‑1 (MQ=255)
                                                                   tGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTc                       >  2:1203298/1‑93 (MQ=255)
                                                                   tGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTc                       >  1:1647455/1‑93 (MQ=255)
                                                                   tGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTc                       >  2:4762190/1‑93 (MQ=255)
                                                                    gATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCt                      <  1:6169033/93‑1 (MQ=255)
                                                                        tcccTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCg                  <  2:1755759/92‑1 (MQ=255)
                                                                        aCCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCg                  >  1:136117/1‑93 (MQ=255)
                                                                           ctgcGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGtt               <  1:2644152/90‑1 (MQ=255)
                                                                           cTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGtt               >  1:1022176/1‑93 (MQ=255)
                                                                           cTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGtt               <  2:3267529/93‑1 (MQ=255)
                                                                            ttCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTc              <  2:4157536/93‑1 (MQ=255)
                                                                            ttCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTc              >  1:1464313/1‑93 (MQ=255)
                                                                            ttCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTc              <  1:2170150/93‑1 (MQ=255)
                                                                            ttCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTc              >  2:711454/1‑93 (MQ=255)
                                                                            ttCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTc              >  1:3848760/1‑93 (MQ=255)
                                                                             tCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCACCTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCg             <  2:6168280/93‑1 (MQ=255)
                                                                             tCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCg             <  2:4633708/93‑1 (MQ=255)
                                                                               gCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGc           <  1:6283243/93‑1 (MQ=255)
                                                                                cccTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCa          >  2:5070409/1‑93 (MQ=255)
                                                                                cccTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCa          <  2:4801101/93‑1 (MQ=255)
                                                                                cccTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCa          >  2:2337320/1‑93 (MQ=255)
                                                                                cccTCCGCCGCGCTCTCAATCGTCAAACGCACGTATAATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCa          <  2:2278951/93‑1 (MQ=255)
                                                                                 ccTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCAt         <  1:1557197/93‑1 (MQ=255)
                                                                                  cTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATc        <  1:2526090/93‑1 (MQ=255)
                                                                                  cTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATc        >  1:3214647/1‑93 (MQ=255)
                                                                                   tGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCCTTTCTGCCGGTTCGGCATcg       >  2:3873798/1‑93 (MQ=255)
                                                                                      gTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATcgccg    >  1:581847/1‑93 (MQ=255)
                                                                                      ctCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATcgccg    <  1:5461647/92‑1 (MQ=255)
                                                                                       tCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATcgccgc   <  1:6027417/93‑1 (MQ=255)
                                                                                       tCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATcgccgc   >  2:3966508/1‑93 (MQ=255)
                                                                                       tCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATcgccgc   >  2:195654/1‑93 (MQ=255)
                                                                                        cgcgCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCt  <  1:2098198/93‑1 (MQ=255)
                                                                                        cgcgCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCt  >  2:5386639/1‑93 (MQ=255)
                                                                                           |                                                                                         
ATTTATTGAATTTGCAG‑ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCT  >  NC_000913/4251553‑4251732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  >  NC_000913/4251550‑4251736
                                                                                             |                                                                                             
GCATTTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCG                                                                                               <  SRR4302533.2130620/94‑1 (MQ=60)
  AATTTATTGAATTTGCAGATTATGGAAGCCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCT                                                                                             <  SRR4302533.5661666/94‑1 (MQ=60)
   ttttatgaaatttgcgtattagGGAAGGCCGGATAAGGCGTTTCCGCCGCAGCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTC                                                                                            <  SRR4302533.396609/72‑1 (MQ=60)
   ATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTC                                                                                            >  SRR4302533.5056865/1‑94 (MQ=60)
   ATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTC                                                                                            >  SRR4302533.5410338/1‑94 (MQ=60)
   ATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTC                                                                                            >  SRR4302533.5516380/1‑94 (MQ=60)
   ATTTATTAAATTGGCAGTTTATGGAAGGCCGAATAAGCCGTTTTCCCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTC                                                                                            <  SRR4302533.2683630/94‑1 (MQ=60)
    TTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGNATCCGGCAATTCTCTCTGATTNNCCNTCGCCCTGCGNCNCGCNCT                                                                                           >  SRR4302533.4542989/1‑94 (MQ=60)
    TTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCT                                                                                           >  SRR4302533.6228829/1‑94 (MQ=60)
    TTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCT                                                                                           >  SRR4302533.5848487/1‑94 (MQ=60)
     TTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTC                                                                                          >  SRR4302533.6218258/1‑94 (MQ=60)
      tatgaatttggagaattagggagggcggaataggggttttcccccccaccgGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCA                                                                                         <  SRR4302533.1182743/43‑1 (MQ=60)
      TATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCA                                                                                         >  SRR4302533.1761805/1‑94 (MQ=60)
       ttattttttggaatggagggaggggggaaacgtgtttttcccccgcctccgcCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAA                                                                                        <  SRR4302533.4324904/42‑1 (MQ=60)
         TGATTTTGCAGATTATGGAAGCCCGTATAAGGCTTTTTCCCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATC                                                                                      <  SRR4302533.1590135/94‑1 (MQ=60)
         TGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATC                                                                                      <  SRR4302533.1925552/94‑1 (MQ=60)
                 CAGATTATGGAAGGCCGGATAAGGCTTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACG                                                                              <  SRR4302533.5621409/94‑1 (MQ=60)
                 CAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACG                                                                              >  SRR4302533.5366862/1‑94 (MQ=60)
                  AGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGC                                                                             >  SRR4302533.2044013/1‑94 (MQ=60)
                  AGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGC                                                                             >  SRR4302533.5446316/1‑94 (MQ=60)
                  AGATTAGGAAAGGCGGGATAGGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGC                                                                             <  SRR4302533.4636705/94‑1 (MQ=60)
                    ATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCAC                                                                           <  SRR4302533.857558/94‑1 (MQ=60)
                     TTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACG                                                                          >  SRR4302533.2359941/1‑94 (MQ=60)
                     TTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACG                                                                          >  SRR4302533.2882501/1‑94 (MQ=60)
                      tggggagggcggaatagggtttttccgccccacccgtCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGT                                                                         <  SRR4302533.2247315/57‑1 (MQ=60)
                        TGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCT                                                                       >  SRR4302533.724535/1‑94 (MQ=60)
                          GAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGA                                                                     >  SRR4302533.1484953/1‑94 (MQ=60)
                          GAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGA                                                                     >  SRR4302533.5762243/1‑94 (MQ=60)
                           AAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGAT                                                                    >  SRR4302533.4056627/1‑94 (MQ=60)
                            AGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATG                                                                   >  SRR4302533.2127306/1‑94 (MQ=60)
                             GGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGT                                                                  >  SRR4302533.2350048/1‑94 (MQ=60)
                             GGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGT                                                                  >  SRR4302533.4329193/1‑94 (MQ=60)
                              GCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTA                                                                 >  SRR4302533.2004989/1‑94 (MQ=60)
                              GCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTA                                                                 >  SRR4302533.4992177/1‑94 (MQ=60)
                               CCGGATAAGGGGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAA                                                                <  SRR4302533.6132019/94‑1 (MQ=60)
                               CCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAA                                                                >  SRR4302533.2662433/1‑94 (MQ=60)
                                CGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAAT                                                               >  SRR4302533.5561341/1‑94 (MQ=60)
                                CGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAAT                                                               >  SRR4302533.2643201/1‑94 (MQ=60)
                                 GGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATC                                                              <  SRR4302533.2116663/94‑1 (MQ=60)
                                 GGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATC                                                              >  SRR4302533.1695271/1‑94 (MQ=60)
                                 GGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATC                                                              >  SRR4302533.5230890/1‑94 (MQ=60)
                                  GATAGGGCTTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCA                                                             <  SRR4302533.329937/94‑1 (MQ=60)
                                  GATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCACACGCACGTCTGATGTAATCA                                                             >  SRR4302533.2383684/1‑94 (MQ=60)
                                  GATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCA                                                             <  SRR4302533.1964156/94‑1 (MQ=60)
                                  GATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCA                                                             <  SRR4302533.619590/94‑1 (MQ=60)
                                  GATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCA                                                             <  SRR4302533.941630/94‑1 (MQ=60)
                                  GATAAGGCGTTTTCGCCCCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCA                                                             <  SRR4302533.982712/94‑1 (MQ=60)
                                  GATAAGACGTATTCGCGGCCTCCGACAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCA                                                             <  SRR4302533.619588/94‑1 (MQ=60)
                                    TAGGGCTTTTTCGCCGCACCGGCCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAAC                                                           <  SRR4302533.1325373/94‑1 (MQ=60)
                                     AAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACT                                                          >  SRR4302533.5054659/1‑94 (MQ=60)
                                         CGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGC                                                      >  SRR4302533.3685414/1‑94 (MQ=60)
                                          GTTTTCGCTGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCC                                                     >  SRR4302533.4713136/1‑94 (MQ=60)
                                          GTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCC                                                     >  SRR4302533.6071667/1‑94 (MQ=60)
                                          GTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCC                                                     >  SRR4302533.672152/1‑94 (MQ=60)
                                          GTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCC                                                     >  SRR4302533.5025811/1‑94 (MQ=60)
                                           tgttcccccCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACTCACGTCTGATGTAATCAACTCCGCCA                                                    <  SRR4302533.219060/85‑1 (MQ=60)
                                            TTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAG                                                   <  SRR4302533.5099333/94‑1 (MQ=60)
                                            TTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAG                                                   <  SRR4302533.4718798/94‑1 (MQ=60)
                                            TTTCGCCGCACCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAG                                                   <  SRR4302533.5672829/94‑1 (MQ=60)
                                             TTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGC                                                  >  SRR4302533.326349/1‑94 (MQ=60)
                                               CGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAA                                                >  SRR4302533.4080797/1‑94 (MQ=60)
                                               CGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAA                                                >  SRR4302533.5257340/1‑94 (MQ=60)
                                                 CCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACT                                              >  SRR4302533.796479/1‑94 (MQ=60)
                                                 CCCCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACT                                              <  SRR4302533.3478872/94‑1 (MQ=60)
                                                  CGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTG                                             >  SRR4302533.1106596/1‑94 (MQ=60)
                                                  CGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTG                                             >  SRR4302533.2477954/1‑94 (MQ=60)
                                                    CATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGAT                                           >  SRR4302533.4296720/1‑94 (MQ=60)
                                                    CATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGAT                                           >  SRR4302533.1864454/1‑94 (MQ=60)
                                                         GGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACC                                      >  SRR4302533.5323509/1‑94 (MQ=60)
                                                           CAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTT                                    <  SRR4302533.577886/94‑1 (MQ=60)
                                                           CAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTT                                    >  SRR4302533.1761099/1‑94 (MQ=60)
                                                            AATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTC                                   >  SRR4302533.2190651/1‑94 (MQ=60)
                                                            AATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTC                                   <  SRR4302533.4634862/94‑1 (MQ=60)
                                                             ATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCA                                  <  SRR4302533.3834826/94‑1 (MQ=60)
                                                             ATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCA                                  >  SRR4302533.1898060/1‑94 (MQ=60)
                                                             ATTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCA                                  <  SRR4302533.6037232/94‑1 (MQ=60)
                                                              TTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAT                                 >  SRR4302533.1752221/1‑94 (MQ=60)
                                                              TTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAT                                 <  SRR4302533.2061603/94‑1 (MQ=60)
                                                              TTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAT                                 <  SRR4302533.5574852/94‑1 (MQ=60)
                                                              TTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAT                                 >  SRR4302533.6093795/1‑94 (MQ=60)
                                                              TTCTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAT                                 >  SRR4302533.3036541/1‑94 (MQ=60)
                                                                CTCTCTGATTCCCCTTCGCCCTGCGTCGCGCGCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCG                               <  SRR4302533.4768915/94‑1 (MQ=60)
                                                                CTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCG                               <  SRR4302533.2824293/94‑1 (MQ=60)
                                                                CTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCG                               >  SRR4302533.27130/1‑94 (MQ=60)
                                                                CTCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCG                               >  SRR4302533.2463841/1‑94 (MQ=60)
                                                                 TCTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGT                              >  SRR4302533.5910363/1‑94 (MQ=60)
                                                                  CTCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTT                             >  SRR4302533.3755835/1‑94 (MQ=60)
                                                                   TCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTT                            >  SRR4302533.2897350/1‑94 (MQ=60)
                                                                   TCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTT                            <  SRR4302533.149276/94‑1 (MQ=60)
                                                                   TCTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTT                            >  SRR4302533.3499588/1‑94 (MQ=60)
                                                                    CTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTC                           >  SRR4302533.3879761/1‑94 (MQ=60)
                                                                    CTGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTC                           >  SRR4302533.1657433/1‑94 (MQ=60)
                                                                     TGATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCT                          <  SRR4302533.1338660/94‑1 (MQ=60)
                                                                      GATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTG                         <  SRR4302533.6206116/94‑1 (MQ=60)
                                                                       ATTACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGC                        <  SRR4302533.953811/94‑1 (MQ=60)
                                                                         TACCCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCG                      >  SRR4302533.136976/1‑94 (MQ=60)
                                                                            CCTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTT                   >  SRR4302533.1028184/1‑94 (MQ=60)
                                                                             CTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTC                  >  SRR4302533.3872262/1‑94 (MQ=60)
                                                                             CTTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTC                  >  SRR4302533.1473138/1‑94 (MQ=60)
                                                                             CTGCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTC                  <  SRR4302533.2660399/94‑1 (MQ=60)
                                                                              TTCGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCG                 <  SRR4302533.2183496/94‑1 (MQ=60)
                                                                                CGCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGC               >  SRR4302533.1449/1‑94 (MQ=60)
                                                                                 GCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCA              <  SRR4302533.6320738/94‑1 (MQ=60)
                                                                                 GCCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCA              >  SRR4302533.3347687/1‑94 (MQ=60)
                                                                                  CCCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCAT             <  SRR4302533.642996/94‑1 (MQ=60)
                                                                                   CCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATC            >  SRR4302533.179089/1‑94 (MQ=60)
                                                                                   CCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATC            <  SRR4302533.1566569/94‑1 (MQ=60)
                                                                                   CCTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATC            >  SRR4302533.3234466/1‑94 (MQ=60)
                                                                                    CTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCG           >  SRR4302533.81867/1‑94 (MQ=60)
                                                                                    CTGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCG           <  SRR4302533.2541642/94‑1 (MQ=60)
                                                                                     TGCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGC          >  SRR4302533.4951721/1‑94 (MQ=60)
                                                                                      GCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCC         <  SRR4302533.2518654/94‑1 (MQ=60)
                                                                                      GCGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCC         <  SRR4302533.3123553/94‑1 (MQ=60)
                                                                                       CGTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCG        >  SRR4302533.585275/1‑94 (MQ=60)
                                                                                        CTCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGC       <  SRR4302533.5494551/94‑1 (MQ=60)
                                                                                         TCGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCT      <  SRR4302533.6063943/94‑1 (MQ=60)
                                                                                         TCGCGCTCTCAATCGGCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAACCCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCT      <  SRR4302533.1252818/94‑1 (MQ=60)
                                                                                          CGCGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTA     <  SRR4302533.2111142/94‑1 (MQ=60)
                                                                                            CGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATC   <  SRR4302533.5991334/94‑1 (MQ=60)
                                                                                            CGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATC   <  SRR4302533.358170/94‑1 (MQ=60)
                                                                                            CGCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATC   >  SRR4302533.5307329/1‑94 (MQ=60)
                                                                                             GCTCTCAATCGTCAAACGCACGTCTGATGTAATaaatcccgcaaggacctataaaacttcctcggttcttccggtttcggagtcgcggtattgc  >  SRR4302533.1588680/1‑33 (MQ=60)
                                                                                             GCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  >  SRR4302533.5053814/1‑94 (MQ=60)
                                                                                             GCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  <  SRR4302533.2741703/94‑1 (MQ=60)
                                                                                             GCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  <  SRR4302533.5994453/94‑1 (MQ=60)
                                                                                             CCTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  <  SRR4302533.4728263/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  >  NC_000913/4251550‑4251736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑