Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,251,782 A→G V755A (GTG→GCG)  plsB ← glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,251,7820AG99.8% 1317.5 / NA 413V755A (GTG→GCG) plsBglycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (187/224);  minor base C (1/0);  total (189/224)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

TGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCG  >  NC_000913/4251692‑4251871
                                                                                          |                                                                                         
tGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGcc                                                                                         <  2:5798635/93‑1 (MQ=255)
 gATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCg                                                                                        <  1:478076/93‑1 (MQ=255)
 gATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCg                                                                                        >  2:4612021/1‑93 (MQ=255)
 gATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCg                                                                                        >  2:940737/1‑93 (MQ=255)
  aTAAACCTTCATCGTTTCTGCCGGTTCGACATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAAcgccgc                                                                                       <  1:9881893/93‑1 (MQ=255)
    aaaCCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCt                                                                                     >  1:256756/1‑93 (MQ=255)
     acccTTCACCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCtt                                                                                    <  1:4408939/91‑1 (MQ=255)
        cTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTc                                                                                 <  1:6314998/93‑1 (MQ=255)
        cTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTc                                                                                 <  2:6514832/93‑1 (MQ=255)
          tCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCga                                                                               <  1:8529479/93‑1 (MQ=255)
           cATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaa                                                                              >  2:8330458/1‑93 (MQ=255)
            aTCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaag                                                                             >  1:7829593/1‑93 (MQ=255)
             tCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaaga                                                                            >  2:3654818/1‑93 (MQ=255)
             tCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaaga                                                                            <  1:1852679/93‑1 (MQ=255)
             tCGTGTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaaga                                                                            <  1:6582750/93‑1 (MQ=255)
             tCGGTTCTGTCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaaga                                                                            <  2:3633995/93‑1 (MQ=255)
              cGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaagaa                                                                           <  1:3067749/93‑1 (MQ=255)
              cGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaagaa                                                                           <  1:968941/93‑1 (MQ=255)
              cGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCgaagaa                                                                           <  1:6046749/93‑1 (MQ=255)
                tttCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACt                                                                         >  1:2901173/1‑93 (MQ=255)
                 ttCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTc                                                                        <  2:8743960/93‑1 (MQ=255)
                  tCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTcc                                                                       <  1:5833837/93‑1 (MQ=255)
                    tGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCgg                                                                     >  2:2434209/1‑93 (MQ=255)
                    tGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCgg                                                                     <  2:5000266/93‑1 (MQ=255)
                    tGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCgg                                                                     <  1:7166401/93‑1 (MQ=255)
                    tGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCgg                                                                     >  2:9059947/1‑93 (MQ=255)
                      ccGGTGCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGgcg                                                                   <  2:1576224/93‑1 (MQ=255)
                         gTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGt                                                                <  1:8080138/93‑1 (MQ=255)
                            cGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGGAGAACTCCGGCGCGTTGa                                                             <  1:3342961/93‑1 (MQ=255)
                            cGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGa                                                             <  1:6919799/93‑1 (MQ=255)
                            cGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGa                                                             <  2:5665120/93‑1 (MQ=255)
                             ggCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAt                                                            <  2:7874916/93‑1 (MQ=255)
                                aTCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGcc                                                         >  1:10006327/1‑93 (MQ=255)
                                 tCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCg                                                        >  2:6847904/1‑93 (MQ=255)
                                 tCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCg                                                        <  2:3716834/93‑1 (MQ=255)
                                 acgccgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCg                                                        <  2:7012209/92‑1 (MQ=255)
                                  cgccgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGt                                                       <  2:9297497/93‑1 (MQ=255)
                                    ccgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCGGCCTTGTCGAAGAACTCCGGCGCGGTGATGCCGTgc                                                     >  1:402145/1‑93 (MQ=255)
                                     cgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgca                                                    >  1:1143936/1‑93 (MQ=255)
                                      gcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcaa                                                   >  1:9305543/1‑92 (MQ=255)
                                       cTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcagc                                                  >  1:1570048/1‑93 (MQ=255)
                                       cTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcagc                                                  <  2:2820622/93‑1 (MQ=255)
                                       cTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcagc                                                  >  2:621503/1‑93 (MQ=255)
                                        tATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcagca                                                 <  1:7414106/93‑1 (MQ=255)
                                         aTCGCTGATATTCCCTTCATCACGCAGTGTCAGCAACAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCAc                                                <  2:4881123/93‑1 (MQ=255)
                                          tCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACg                                               <  2:7695209/93‑1 (MQ=255)
                                          tCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACg                                               >  2:7386383/1‑93 (MQ=255)
                                            gCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGga                                             <  2:2143385/93‑1 (MQ=255)
                                             cTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgag                                            <  1:6993020/93‑1 (MQ=255)
                                             cTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgag                                            >  2:192780/1‑93 (MQ=255)
                                             cTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgag                                            <  1:8782479/93‑1 (MQ=255)
                                              tGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgaga                                           <  1:9087873/93‑1 (MQ=255)
                                              tGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgaga                                           >  1:3773098/1‑93 (MQ=255)
                                              tGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgaga                                           <  1:2122702/93‑1 (MQ=255)
                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                          <  2:5267932/93‑1 (MQ=255)
                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                          >  1:5177195/1‑93 (MQ=255)
                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                          <  2:3881400/93‑1 (MQ=255)
                                               gATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagag                                          >  1:2073343/1‑93 (MQ=255)
                                                atatACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGgagaga                                         <  1:2287636/93‑1 (MQ=255)
                                                   tgcccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGt                                      <  2:10007102/91‑1 (MQ=255)
                                                   taCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGt                                      <  2:2723726/93‑1 (MQ=255)
                                                    aCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGtt                                     <  1:6406491/93‑1 (MQ=255)
                                                    aCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGtt                                     <  1:7240693/93‑1 (MQ=255)
                                                    aCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGtt                                     <  2:558132/93‑1 (MQ=255)
                                                    aCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGtt                                     >  1:1313649/1‑93 (MQ=255)
                                                     cccTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTg                                    <  2:3233652/93‑1 (MQ=255)
                                                        ttCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgcgc                                 >  1:7342743/1‑93 (MQ=255)
                                                         tcatcaCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGcgcg                                >  2:6350934/1‑93 (MQ=255)
                                                         ccatcaCGCAGTGTCAGCATCAGAGAACTGAATGCCGCCTTGTCGAAGAACTCCGGCGCTTTGAAGCCTTTCATCTCGGAGAGACGTTGcgcg                                >  1:8974678/2‑93 (MQ=255)
                                                           atcaCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGag                              >  1:7554767/1‑92 (MQ=255)
                                                           atcaCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGAc                              >  2:2564128/1‑93 (MQ=255)
                                                            tcaCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACg                             >  2:9702564/1‑93 (MQ=255)
                                                             caCGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACgg                            <  2:9761146/93‑1 (MQ=255)
                                                               cGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTg                          <  2:4013446/93‑1 (MQ=255)
                                                                gCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGACGAACTCCGGCGCGTTCATGCCGTGCCGCAACGAGAGACGTTGCTCGACgggcg                         >  2:1415936/1‑90 (MQ=255)
                                                                gCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGc                         <  1:1351692/93‑1 (MQ=255)
                                                                gCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGc                         <  2:6617334/93‑1 (MQ=255)
                                                                 cAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCg                        <  1:1687875/93‑1 (MQ=255)
                                                                  aGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCgg                       >  2:1025732/1‑93 (MQ=255)
                                                                   gtgtCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGc                      <  2:8915226/93‑1 (MQ=255)
                                                                    tgtCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCTCGACGGAGCGGcc                     >  2:4261128/1‑92 (MQ=255)
                                                                    tgtCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGct                     >  1:8320751/1‑93 (MQ=255)
                                                                    tgtCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGcg                     >  2:5484421/1‑92 (MQ=255)
                                                                      tCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctct                   >  2:4431796/1‑93 (MQ=255)
                                                                      tCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctct                   >  2:2030247/1‑93 (MQ=255)
                                                                        aGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGctctct                 <  1:5744816/93‑1 (MQ=255)
                                                                           aCCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtc              <  1:498811/93‑1 (MQ=255)
                                                                            ccAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtct             >  1:3232331/1‑93 (MQ=255)
                                                                             cAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtctc            >  2:7247847/1‑93 (MQ=255)
                                                                              agagAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTcc           <  2:8202427/93‑1 (MQ=255)
                                                                               gagaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCa          <  1:4125090/93‑1 (MQ=255)
                                                                               gagaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCa          >  1:3641951/1‑93 (MQ=255)
                                                                                 gaACTGACCGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGc        <  2:51068/93‑1 (MQ=255)
                                                                                 gaACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGCGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGc        >  1:3562501/1‑93 (MQ=255)
                                                                                    cTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTa     <  2:1162028/93‑1 (MQ=255)
                                                                                    cTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTa     >  1:5269798/1‑93 (MQ=255)
                                                                                    cTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTa     <  2:9643032/93‑1 (MQ=255)
                                                                                     tGAACGCCGCCTTGTCGAAGACCTCCGGCGCGTTGATGCCGTGCAGCACGGATAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAc    <  2:6000597/93‑1 (MQ=255)
                                                                                     tGAACGCCGCCTTGTCGAAGAACTCCGGCGCGCTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGATCCCTTTCTCCACCGTAc    >  2:2279476/1‑93 (MQ=255)
                                                                                      gAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAcc   <  1:4543206/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCG  >  NC_000913/4251692‑4251871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 223 total aligned reads displayed.
AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGT  >  NC_000913/4251689‑4251875
                                                                                             |                                                                                             
AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATGCCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACG                                                                                               <  SRR4302540.7396089/94‑1 (MQ=60)
AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACG                                                                                               >  SRR4302540.1083218/1‑94 (MQ=60)
AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACC                                                                                               >  SRR4302540.2164065/1‑94 (MQ=60)
 ACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGC                                                                                              >  SRR4302540.10126152/1‑94 (MQ=60)
 ACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGC                                                                                              >  SRR4302540.4981249/1‑94 (MQ=60)
  CTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGTTATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCC                                                                                             <  SRR4302540.5223810/94‑1 (MQ=60)
   TGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCG                                                                                            >  SRR4302540.9969010/1‑94 (MQ=60)
    GATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGC                                                                                           >  SRR4302540.3109209/1‑94 (MQ=60)
    GATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGC                                                                                           <  SRR4302540.9133771/94‑1 (MQ=60)
    GATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGC                                                                                           <  SRR4302540.483195/94‑1 (MQ=60)
     ATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCC                                                                                          >  SRR4302540.7002597/1‑94 (MQ=60)
     ATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCC                                                                                          >  SRR4302540.7141417/1‑94 (MQ=60)
     ATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACCCCGCC                                                                                          >  SRR4302540.950556/1‑94 (MQ=60)
      TAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCT                                                                                         <  SRR4302540.2449226/94‑1 (MQ=60)
      TAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCT                                                                                         >  SRR4302540.259244/1‑94 (MQ=60)
      TAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCT                                                                                         >  SRR4302540.2127222/1‑94 (MQ=60)
      TAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCT                                                                                         <  SRR4302540.6441872/94‑1 (MQ=60)
      TAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCT                                                                                         <  SRR4302540.6106374/94‑1 (MQ=60)
       AAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTT                                                                                        <  SRR4302540.6180903/94‑1 (MQ=60)
        ACCCTTCACCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTG                                                                                       <  SRR4302540.4456734/94‑1 (MQ=60)
           CTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCG                                                                                    <  SRR4302540.7004592/94‑1 (MQ=60)
           CTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCG                                                                                    <  SRR4302540.8503915/94‑1 (MQ=60)
             TCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAA                                                                                  <  SRR4302540.8628585/94‑1 (MQ=60)
             TCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAA                                                                                  >  SRR4302540.8717627/1‑94 (MQ=60)
              CATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAG                                                                                 >  SRR4302540.8241216/1‑94 (MQ=60)
              CATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAG                                                                                 >  SRR4302540.7921080/1‑94 (MQ=60)
               ATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGA                                                                                <  SRR4302540.1692453/94‑1 (MQ=60)
               ATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGA                                                                                <  SRR4302540.4067174/94‑1 (MQ=60)
               ATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGA                                                                                <  SRR4302540.4476843/94‑1 (MQ=60)
               ATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGA                                                                                >  SRR4302540.471757/1‑94 (MQ=60)
                TCGTTTCTGCCGGTTCGTCATCGCCGCTATCGCTGATATACCCGTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAA                                                                               <  SRR4302540.6512271/94‑1 (MQ=60)
                TCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAA                                                                               <  SRR4302540.1872998/94‑1 (MQ=60)
                TCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAA                                                                               <  SRR4302540.886828/94‑1 (MQ=60)
                TCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAA                                                                               <  SRR4302540.5316514/94‑1 (MQ=60)
                TCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAA                                                                               <  SRR4302540.1591344/94‑1 (MQ=60)
                TCGTGTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAA                                                                               <  SRR4302540.6655665/94‑1 (MQ=60)
                 CGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAAC                                                                              <  SRR4302540.978922/94‑1 (MQ=60)
                 CGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAAC                                                                              <  SRR4302540.6113926/94‑1 (MQ=60)
                  GTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACT                                                                             <  SRR4302540.344711/94‑1 (MQ=60)
                  GTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACT                                                                             >  SRR4302540.2932836/1‑94 (MQ=60)
                    TTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCC                                                                           <  SRR4302540.3982787/94‑1 (MQ=60)
                     TCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCG                                                                          <  SRR4302540.5898345/94‑1 (MQ=60)
                     TCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCG                                                                          <  SRR4302540.3940830/94‑1 (MQ=60)
                      CTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTacgc                                                                         >  SRR4302540.100008/1‑90 (MQ=60)
                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCCTCGCGCAGTGGACGCACGAGAGAACTGAACGGCGCCTTGTCGAAcacctgcggc                                                                        >  SRR4302540.6807847/1‑84 (MQ=46)
                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGC                                                                        >  SRR4302540.10107925/1‑94 (MQ=60)
                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGC                                                                        >  SRR4302540.6807846/1‑94 (MQ=60)
                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGC                                                                        <  SRR4302540.7246297/94‑1 (MQ=60)
                       TGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGC                                                                        <  SRR4302540.305918/94‑1 (MQ=60)
                        GCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCG                                                                       <  SRR4302540.4027882/94‑1 (MQ=60)
                         CCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGC                                                                      <  SRR4302540.3660289/94‑1 (MQ=60)
                          CGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCG                                                                     <  SRR4302540.1720315/94‑1 (MQ=60)
                          CGGTGCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCG                                                                     <  SRR4302540.1720319/94‑1 (MQ=60)
                            GTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTT                                                                   >  SRR4302540.4490869/1‑94 (MQ=60)
                            GTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTT                                                                   <  SRR4302540.8173921/94‑1 (MQ=60)
                            GTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTT                                                                   >  SRR4302540.2892023/1‑94 (MQ=60)
                            GTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTT                                                                   <  SRR4302540.4437750/94‑1 (MQ=60)
                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGGAGAACTCCGGCGCGTTGAT                                                                <  SRR4302540.3379642/94‑1 (MQ=60)
                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                <  SRR4302540.6996797/94‑1 (MQ=60)
                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                <  SRR4302540.6067139/94‑1 (MQ=60)
                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                >  SRR4302540.4601555/1‑94 (MQ=60)
                               CGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGAT                                                                >  SRR4302540.9989577/1‑94 (MQ=60)
                                GGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATG                                                               <  SRR4302540.7974425/94‑1 (MQ=60)
                                GGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATG                                                               >  SRR4302540.6402765/1‑94 (MQ=60)
                                GGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATG                                                               >  SRR4302540.1672770/1‑94 (MQ=60)
                                 GCATCGCCGCTATCGCTTATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGC                                                              >  SRR4302540.9458235/1‑94 (MQ=60)
                                 GCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGC                                                              <  SRR4302540.1164742/94‑1 (MQ=60)
                                  CATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCC                                                             >  SRR4302540.4305021/1‑94 (MQ=60)
                                  CATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCC                                                             >  SRR4302540.8695297/1‑94 (MQ=60)
                                  CATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCC                                                             >  SRR4302540.10122867/1‑94 (MQ=60)
                                    TCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGT                                                           <  SRR4302540.721964/94‑1 (MQ=60)
                                    TCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGT                                                           <  SRR4302540.5820020/94‑1 (MQ=60)
                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCGGCCTTGTCGAAGAACTCCGGCGCGGTGATGCCGTGC                                                         >  SRR4302540.406340/1‑94 (MQ=60)
                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGC                                                         <  SRR4302540.3989089/94‑1 (MQ=60)
                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGC                                                         >  SRR4302540.2033072/1‑94 (MQ=60)
                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGC                                                         >  SRR4302540.9350299/1‑94 (MQ=60)
                                      GCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGC                                                         >  SRR4302540.9148681/1‑94 (MQ=60)
                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       <  SRR4302540.347763/94‑1 (MQ=60)
                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       <  SRR4302540.7888484/94‑1 (MQ=60)
                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAG                                                       >  SRR4302540.4740692/1‑94 (MQ=60)
                                        CGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAA                                                       >  SRR4302540.9416674/1‑94 (MQ=60)
                                         GCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGC                                                      >  SRR4302540.1587257/1‑94 (MQ=60)
                                         GCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGC                                                      >  SRR4302540.5326555/1‑94 (MQ=60)
                                         GCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGC                                                      <  SRR4302540.5191588/94‑1 (MQ=60)
                                         GCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGC                                                      >  SRR4302540.7829886/1‑94 (MQ=60)
                                          CTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCA                                                     >  SRR4302540.1068251/1‑94 (MQ=60)
                                          CTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCA                                                     <  SRR4302540.9142931/94‑1 (MQ=60)
                                          CTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCA                                                     >  SRR4302540.3679280/1‑94 (MQ=60)
                                          CTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCGTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCA                                                     >  SRR4302540.8561019/1‑94 (MQ=60)
                                           TATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCAC                                                    >  SRR4302540.7065507/1‑94 (MQ=60)
                                           TATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCAC                                                    >  SRR4302540.1344929/1‑94 (MQ=60)
                                           TATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCAC                                                    <  SRR4302540.4939883/94‑1 (MQ=60)
                                           TATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCAC                                                    <  SRR4302540.7496688/94‑1 (MQ=60)
                                           TATCGCGCATATACCCTGCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCAC                                                    <  SRR4302540.7496689/94‑1 (MQ=60)
                                            ATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTAGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACG                                                   <  SRR4302540.2942508/94‑1 (MQ=60)
                                              CGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGA                                                 <  SRR4302540.7363261/94‑1 (MQ=60)
                                              CGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGA                                                 <  SRR4302540.6476900/94‑1 (MQ=60)
                                               GCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAG                                                <  SRR4302540.5880531/94‑1 (MQ=60)
                                               GCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAG                                                <  SRR4302540.9284841/94‑1 (MQ=60)
                                               GCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAG                                                <  SRR4302540.3297886/94‑1 (MQ=60)
                                                CTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGA                                               <  SRR4302540.7070637/94‑1 (MQ=60)
                                                CTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGA                                               >  SRR4302540.3814268/1‑94 (MQ=60)
                                                CTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGA                                               <  SRR4302540.8884148/94‑1 (MQ=60)
                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              <  SRR4302540.8150080/94‑1 (MQ=60)
                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              <  SRR4302540.9197048/94‑1 (MQ=60)
                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              <  SRR4302540.2145474/94‑1 (MQ=60)
                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              >  SRR4302540.5233543/1‑94 (MQ=60)
                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              >  SRR4302540.2095637/1‑94 (MQ=60)
                                                 TGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAG                                              <  SRR4302540.4299422/94‑1 (MQ=60)
                                                  GATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGA                                             >  SRR4302540.3296672/1‑94 (MQ=60)
                                                  GATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGA                                             >  SRR4302540.2306116/1‑94 (MQ=60)
                                                  GATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGA                                             <  SRR4302540.2030002/94‑1 (MQ=60)
                                                   ATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGAC                                            <  SRR4302540.9431287/94‑1 (MQ=60)
                                                   ATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGAC                                            <  SRR4302540.2312237/94‑1 (MQ=60)
                                                   ATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGAC                                            <  SRR4302540.1328773/94‑1 (MQ=60)
                                                   ATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGAC                                            <  SRR4302540.3700774/94‑1 (MQ=60)
                                                   ATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGAGTTGATGCCGTGCAGCCCGGAGAGAC                                            >  SRR4302540.1350762/1‑94 (MQ=60)
                                                    TATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACG                                           <  SRR4302540.8371241/94‑1 (MQ=60)
                                                     ATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGT                                          <  SRR4302540.1341550/94‑1 (MQ=60)
                                                     ATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGT                                          <  SRR4302540.1030503/94‑1 (MQ=60)
                                                      TACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTT                                         >  SRR4302540.1327302/1‑94 (MQ=60)
                                                      TACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTT                                         <  SRR4302540.628117/94‑1 (MQ=60)
                                                      TACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTT                                         >  SRR4302540.1327301/1‑94 (MQ=60)
                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        >  SRR4302540.2264524/1‑94 (MQ=60)
                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        >  SRR4302540.797847/1‑94 (MQ=60)
                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        <  SRR4302540.7291382/94‑1 (MQ=60)
                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        <  SRR4302540.6477798/94‑1 (MQ=60)
                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        <  SRR4302540.7321458/94‑1 (MQ=60)
                                                       ACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTG                                        <  SRR4302540.7321459/94‑1 (MQ=60)
                                                        CCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGC                                       <  SRR4302540.429494/94‑1 (MQ=60)
                                                         CCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCG                                      <  SRR4302540.7438637/94‑1 (MQ=60)
                                                          CTTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGC                                     >  SRR4302540.7424669/1‑94 (MQ=60)
                                                           TTCATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCG                                    <  SRR4302540.2433559/94‑1 (MQ=60)
                                                           TCCATCACGCAGTGTCAGCATCAGAGAACTGAATGCCGCCTTGTCGAAGAACTCCGGCGCTTTGAAGCCTTTCATCTCGGAGAGACGTTGCGCG                                    >  SRR4302540.9081399/1‑94 (MQ=48)
                                                             TATCACGCAGTGTTAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGAC                                  >  SRR4302540.7639480/1‑94 (MQ=60)
                                                             TATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGAG                                  >  SRR4302540.7639481/1‑94 (MQ=60)
                                                              ATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACG                                 <  SRR4302540.5479257/94‑1 (MQ=60)
                                                              ATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACG                                 >  SRR4302540.5243681/1‑94 (MQ=60)
                                                              ATCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACG                                 <  SRR4302540.10147654/94‑1 (MQ=60)
                                                               TCACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGG                                <  SRR4302540.3074070/94‑1 (MQ=60)
                                                                CACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGT                               <  SRR4302540.8337646/94‑1 (MQ=60)
                                                                CACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGT                               <  SRR4302540.1495659/94‑1 (MQ=60)
                                                                CACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGT                               >  SRR4302540.5137280/1‑94 (MQ=60)
                                                                CACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGT                               <  SRR4302540.5225798/94‑1 (MQ=60)
                                                                 ACGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTG                              <  SRR4302540.2127763/94‑1 (MQ=60)
                                                                  CGCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGC                             <  SRR4302540.9359892/94‑1 (MQ=60)
                                                                   GCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCG                            <  SRR4302540.1365907/94‑1 (MQ=60)
                                                                   GCAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCG                            <  SRR4302540.148849/94‑1 (MQ=60)
                                                                    CAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGG                           >  SRR4302540.3787007/1‑94 (MQ=60)
                                                                    CAGTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGG                           <  SRR4302540.1706181/94‑1 (MQ=60)
                                                                      GTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCT                         <  SRR4302540.5640792/94‑1 (MQ=60)
                                                                      GTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCT                         >  SRR4302540.8417336/1‑94 (MQ=60)
                                                                      GTGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAAAGACGTTGCGCGACGGTGCGGCT                         >  SRR4302540.6159322/1‑94 (MQ=60)
                                                                       TGTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTC                        >  SRR4302540.3480601/1‑94 (MQ=60)
                                                                        GTCAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCT                       <  SRR4302540.7653497/94‑1 (MQ=60)
                                                                          CAGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCT                     >  SRR4302540.3670313/1‑94 (MQ=60)
                                                                           AGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTT                    >  SRR4302540.7435373/1‑94 (MQ=60)
                                                                           AGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTT                    >  SRR4302540.3354651/1‑94 (MQ=60)
                                                                           AGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTT                    <  SRR4302540.5808155/94‑1 (MQ=60)
                                                                           AGCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTT                    <  SRR4302540.6741788/94‑1 (MQ=60)
                                                                            GCACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTT                   <  SRR4302540.10059966/94‑1 (MQ=60)
                                                                             CACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTC                  <  SRR4302540.3108424/94‑1 (MQ=60)
                                                                             CACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTC                  >  SRR4302540.3189446/1‑94 (MQ=60)
                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 <  SRR4302540.504156/94‑1 (MQ=60)
                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 >  SRR4302540.5537785/1‑94 (MQ=60)
                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 <  SRR4302540.5857767/94‑1 (MQ=60)
                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 >  SRR4302540.3267480/1‑94 (MQ=60)
                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCT                 >  SRR4302540.8981453/1‑94 (MQ=60)
                                                                              ACCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGAGGCTCTCTTTCC                 >  SRR4302540.1984977/1‑94 (MQ=60)
                                                                               CCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTC                >  SRR4302540.4958675/1‑94 (MQ=60)
                                                                               CCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTC                >  SRR4302540.5784517/1‑94 (MQ=60)
                                                                               CCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTC                >  SRR4302540.1762895/1‑94 (MQ=60)
                                                                               CCAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTC                >  SRR4302540.7395198/1‑94 (MQ=60)
                                                                                CAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCC               <  SRR4302540.4100021/94‑1 (MQ=60)
                                                                                CAGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCC               >  SRR4302540.9991663/1‑94 (MQ=60)
                                                                                 AGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCA              >  SRR4302540.3681836/1‑94 (MQ=60)
                                                                                 AGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCA              <  SRR4302540.2670308/94‑1 (MQ=60)
                                                                                 AGAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCA              <  SRR4302540.4948232/94‑1 (MQ=60)
                                                                                  GAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAG             <  SRR4302540.1166044/94‑1 (MQ=60)
                                                                                  GAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAG             >  SRR4302540.8978849/1‑94 (MQ=60)
                                                                                  GAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAG             <  SRR4302540.4169648/94‑1 (MQ=60)
                                                                                  GAGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAG             <  SRR4302540.230074/94‑1 (MQ=60)
                                                                                   AGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGCGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGC            >  SRR4302540.3601418/1‑94 (MQ=60)
                                                                                   AGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGCCGGTGCGGCTCTCTTTCTCCCGC            >  SRR4302540.7257932/1‑94 (MQ=60)
                                                                                   AGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAcg            >  SRR4302540.7288813/1‑92 (MQ=60)
                                                                                   AGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGC            <  SRR4302540.7630197/94‑1 (MQ=60)
                                                                                   AGAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGC            >  SRR4302540.7288815/1‑94 (MQ=60)
                                                                                    GAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCG           >  SRR4302540.2267044/1‑94 (MQ=60)
                                                                                    GAACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCG           >  SRR4302540.6596581/1‑94 (MQ=60)
                                                                                     AACTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCTT          >  SRR4302540.7493268/1‑94 (MQ=60)
                                                                                       CTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAC        >  SRR4302540.2937176/1‑94 (MQ=60)
                                                                                       CTGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTAC        >  SRR4302540.8700094/1‑94 (MQ=60)
                                                                                        TGAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACC       >  SRR4302540.6280926/1‑94 (MQ=60)
                                                                                         GAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCG      <  SRR4302540.2864327/94‑1 (MQ=60)
                                                                                         GAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCG      <  SRR4302540.656274/94‑1 (MQ=60)
                                                                                         GAACGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCG      <  SRR4302540.4592369/94‑1 (MQ=60)
                                                                                            CGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGG   <  SRR4302540.6925355/94‑1 (MQ=60)
                                                                                            CGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGG   >  SRR4302540.6022791/1‑94 (MQ=60)
                                                                                            CGCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGG   >  SRR4302540.8745767/1‑94 (MQ=60)
                                                                                             GCCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGT  >  SRR4302540.8498779/1‑94 (MQ=60)
                                                                                             |                                                                                             
AACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGT  >  NC_000913/4251689‑4251875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑