Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,235 A→G C406R (TGC→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2350AG99.5% 690.2 / NA 218C406R (TGC→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (112/105);  minor base T (0/1);  total (112/106)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324
                                                                                          |                                                                                         
tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                                         >  1:2784241/1‑93 (MQ=255)
tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                                         <  1:4475127/93‑1 (MQ=255)
tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                                         >  1:1641337/1‑93 (MQ=255)
 gCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCgg                                                                                        <  2:3016288/93‑1 (MQ=255)
  cTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGa                                                                                       <  2:4805105/93‑1 (MQ=255)
     aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                                                    <  1:5830973/93‑1 (MQ=255)
       gTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAtt                                                                                  <  1:2152682/93‑1 (MQ=255)
        tCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                                                 <  2:4718240/93‑1 (MQ=255)
          ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                                               >  1:4959162/1‑93 (MQ=255)
          ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                                               <  1:3544358/93‑1 (MQ=255)
           gagaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGGGGACGATTACTg                                                                              >  2:1714216/1‑93 (MQ=255)
           gagaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTg                                                                              >  1:5628996/1‑93 (MQ=255)
            agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             <  2:3951812/93‑1 (MQ=255)
              aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                                           >  1:2236382/1‑93 (MQ=255)
                  caggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACt                                                                       >  1:1400009/1‑93 (MQ=255)
                  caggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACt                                                                       <  1:5228605/93‑1 (MQ=255)
                   aggcagCGTCGTATTCTCGACATACCCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCATCTGGCGCGCGGACGATTACTGATAAACtt                                                                      <  2:807117/93‑1 (MQ=255)
                    ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                                                     <  2:6127892/93‑1 (MQ=255)
                      cagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGc                                                                   >  1:1176867/1‑93 (MQ=255)
                      cagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGc                                                                   <  1:3110949/93‑1 (MQ=255)
                       agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCt                                                                  >  1:2429432/1‑93 (MQ=255)
                        gcggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTa                                                                 >  1:1721735/1‑93 (MQ=255)
                        gcggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTa                                                                 >  1:2081170/1‑93 (MQ=255)
                         cggcgTATTCTCAACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTaa                                                                <  2:3674716/93‑1 (MQ=255)
                          ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                                               <  1:1691967/93‑1 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              >  2:1763595/1‑93 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              >  1:5732053/1‑93 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              >  1:2680855/1‑93 (MQ=255)
                            cgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttc                                                             <  2:5203070/93‑1 (MQ=255)
                             gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                                            >  1:4992653/1‑93 (MQ=255)
                              tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           >  1:4512352/1‑93 (MQ=255)
                              tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           >  1:4566380/1‑93 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  2:746659/93‑1 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  1:3257328/93‑1 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  2:5927779/93‑1 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  2:556951/93‑1 (MQ=255)
                                ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                                         >  1:1247355/1‑93 (MQ=255)
                                ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                                         >  1:4655472/1‑93 (MQ=255)
                                ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                                         >  1:183620/1‑93 (MQ=255)
                                 tctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgc                                                        >  2:4482899/1‑93 (MQ=255)
                                   tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                                                      >  1:5499982/1‑93 (MQ=255)
                                     gACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAc                                                    >  2:1518637/1‑93 (MQ=255)
                                      aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                                                   >  1:3855333/1‑93 (MQ=255)
                                      aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                                                   >  2:4197688/1‑93 (MQ=255)
                                        aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                                                 <  1:3055238/93‑1 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                                                >  2:1920976/1‑93 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                                                >  1:4146917/1‑93 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                                                >  1:2706766/1‑93 (MQ=255)
                                          acacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCgcgc                                               >  2:2166150/1‑93 (MQ=255)
                                          acacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCgcgc                                               >  1:958031/1‑93 (MQ=255)
                                             cGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTgg                                            <  1:1587720/93‑1 (MQ=255)
                                             cGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTgg                                            <  1:809967/93‑1 (MQ=255)
                                              gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGa                                           <  1:3888495/93‑1 (MQ=255)
                                              gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGa                                           >  2:424478/1‑93 (MQ=255)
                                                ggCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAtt                                         >  1:3411111/1‑93 (MQ=255)
                                                 gcgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTc                                        <  2:4896188/93‑1 (MQ=255)
                                                  cgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTcc                                       <  2:2273763/93‑1 (MQ=255)
                                                   gcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCg                                      >  1:4224621/1‑93 (MQ=255)
                                                    cgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCTCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGt                                     <  1:5374861/93‑1 (MQ=255)
                                                      cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTTGCGGACCGCGCTGGGTTTCGgct                                   >  2:1094020/1‑93 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATTAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  >  2:5798178/1‑93 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  <  1:4236067/93‑1 (MQ=255)
                                                        tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct                                 <  1:860968/93‑1 (MQ=255)
                                                         cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt                                <  1:1463862/93‑1 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTTCGTCTCttt                               >  2:5341906/1‑93 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTTCGTCTCttt                               >  2:2684398/1‑93 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               <  2:3279485/93‑1 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               <  1:3431034/93‑1 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCTCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta                              <  1:1047951/93‑1 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCTTCTCTTta                              <  2:6164184/93‑1 (MQ=255)
                                                            gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat                             <  2:5876933/93‑1 (MQ=255)
                                                             aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata                            <  1:5053926/93‑1 (MQ=255)
                                                              cGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAg                           <  1:4242398/93‑1 (MQ=255)
                                                               gCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgc                          <  1:6144676/93‑1 (MQ=255)
                                                                cAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcg                         <  1:2566767/93‑1 (MQ=255)
                                                                  ggcgcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTcc                                       <  2:2872448/75‑1 (MQ=255)
                                                                    cgcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGgcgc                     <  1:3993414/93‑1 (MQ=255)
                                                                     gcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCt                    >  2:6022052/1‑93 (MQ=255)
                                                                     gcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCt                    >  2:5489119/1‑93 (MQ=255)
                                                                     gcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCt                    >  1:155067/1‑93 (MQ=255)
                                                                      cgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTc                   <  1:3017230/93‑1 (MQ=255)
                                                                      cgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTc                   <  2:5745315/93‑1 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCTGCGCTCCa                 >  1:5461608/1‑93 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               <  1:470827/93‑1 (MQ=255)
                                                                           cTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCatat              <  2:3497704/93‑1 (MQ=255)
                                                                            ttCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatt             >  2:1901452/1‑93 (MQ=255)
                                                                            ttCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatt             >  2:1557552/1‑93 (MQ=255)
                                                                             tCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatta            <  2:5108586/93‑1 (MQ=255)
                                                                             tCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatta            >  1:5092586/1‑93 (MQ=255)
                                                                              cGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtattat           <  2:3592778/93‑1 (MQ=255)
                                                                               gCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATg          <  1:734326/93‑1 (MQ=255)
                                                                                cAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgc         <  2:2338544/93‑1 (MQ=255)
                                                                                  gccggCGCGCGGATGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcag       >  2:2888482/4‑93 (MQ=255)
                                                                                  gCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcag       >  2:1423712/1‑93 (MQ=255)
                                                                                   cTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagc      >  1:4861599/1‑93 (MQ=255)
                                                                                    tGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagca     >  2:1073906/1‑93 (MQ=255)
                                                                                    tGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagca     >  1:2412627/1‑93 (MQ=255)
                                                                                      gcgcgcGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACg   <  2:378081/93‑1 (MQ=255)
                                                                                       cgcgcgGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGc  <  2:1262720/93‑1 (MQ=255)
                                                                                       cgcgcgGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGc  <  1:5585337/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270142‑4270328
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CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                                               >  SRR4302533.380355/1‑94 (MQ=60)
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                                               >  SRR4302533.1572348/1‑94 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCTGCGTATTCTCGACATACACGTGGCGCGCTTCCCGCCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             <  SRR4302533.618938/94‑1 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             >  SRR4302533.2801348/1‑94 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             >  SRR4302533.1651281/1‑94 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             <  SRR4302533.618937/94‑1 (MQ=60)
   TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                                            <  SRR4302533.4502188/94‑1 (MQ=60)
   TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                                            <  SRR4302533.1590378/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302533.5251872/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302533.812134/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302533.5569476/94‑1 (MQ=60)
      CCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                                         <  SRR4302533.1061547/94‑1 (MQ=60)
       CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGA                                                                                        >  SRR4302533.4916115/1‑94 (MQ=60)
       CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGA                                                                                        >  SRR4302533.312244/1‑94 (MQ=60)
        ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       <  SRR4302533.5866216/94‑1 (MQ=60)
          GTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTA                                                                                     <  SRR4302533.1468175/94‑1 (MQ=60)
          GTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTA                                                                                     <  SRR4302533.2165942/94‑1 (MQ=60)
            CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                                                   <  SRR4302533.1459069/94‑1 (MQ=60)
            CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                                                   >  SRR4302533.4989206/1‑94 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  <  SRR4302533.3566204/94‑1 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  >  SRR4302533.5663036/1‑94 (MQ=60)
              GAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGA                                                                                 <  SRR4302533.2695305/94‑1 (MQ=60)
               AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                <  SRR4302533.1746075/94‑1 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               <  SRR4302533.5095554/94‑1 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               >  SRR4302533.2250207/1‑94 (MQ=60)
                    GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                                           >  SRR4302533.1408447/1‑94 (MQ=60)
                    GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                                           >  SRR4302533.5869196/1‑94 (MQ=60)
                     CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                                          <  SRR4302533.5260059/94‑1 (MQ=60)
                     CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                                          >  SRR4302533.1870606/1‑94 (MQ=60)
                      AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTG                                                                         >  SRR4302533.4219470/1‑94 (MQ=60)
                       GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                                                        <  SRR4302533.2340725/94‑1 (MQ=60)
                        GCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGC                                                                       >  SRR4302533.1183929/1‑94 (MQ=60)
                         CAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCT                                                                      >  SRR4302533.2444449/1‑94 (MQ=60)
                         CAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCT                                                                      <  SRR4302533.3130073/94‑1 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     >  SRR4302533.5400546/1‑94 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     >  SRR4302533.2093977/1‑94 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     >  SRR4302533.1732176/1‑94 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     >  SRR4302533.504418/1‑94 (MQ=60)
                           GCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAA                                                                    <  SRR4302533.5206634/94‑1 (MQ=60)
                            CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                                                   <  SRR4302533.406857/94‑1 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302533.2407134/1‑94 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302533.2697281/1‑94 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302533.4512983/1‑94 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  <  SRR4302533.1702215/94‑1 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302533.5766658/1‑94 (MQ=60)
                              GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                                                 >  SRR4302533.79532/1‑94 (MQ=60)
                              GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                                                 >  SRR4302533.4561310/1‑94 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302533.3903970/94‑1 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                >  SRR4302533.5022923/1‑94 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302533.4594065/1‑94 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302533.4539677/1‑94 (MQ=60)
                                 TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              >  SRR4302533.2956637/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302533.5751654/94‑1 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302533.4683683/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302533.3277417/94‑1 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302533.4902720/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302533.184762/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302533.1623044/94‑1 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302533.1254804/1‑94 (MQ=60)
                                     CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          >  SRR4302533.5533160/1‑94 (MQ=60)
                                      TCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTTGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTA                                                         <  SRR4302533.4461101/94‑1 (MQ=60)
                                       CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                                                        <  SRR4302533.3542412/94‑1 (MQ=60)
                                        GACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACC                                                       >  SRR4302533.3878875/1‑94 (MQ=60)
                                           ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    >  SRR4302533.1384606/1‑94 (MQ=60)
                                           ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    <  SRR4302533.3073966/94‑1 (MQ=60)
                                           ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    >  SRR4302533.4172043/1‑94 (MQ=60)
                                           ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    >  SRR4302533.2723371/1‑94 (MQ=60)
                                           ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    >  SRR4302533.4724447/1‑94 (MQ=60)
                                            TACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGC                                                   >  SRR4302533.963664/1‑94 (MQ=60)
                                              CGCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTG                                                 <  SRR4302533.3739512/94‑1 (MQ=60)
                                              CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTGCCGCGCTG                                                 <  SRR4302533.5429198/94‑1 (MQ=60)
                                               ACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGG                                                >  SRR4302533.3373669/1‑94 (MQ=60)
                                                CGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGA                                               <  SRR4302533.814721/94‑1 (MQ=60)
                                                CGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGA                                               <  SRR4302533.1682378/94‑1 (MQ=60)
                                                CGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGA                                               <  SRR4302533.1597305/94‑1 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              <  SRR4302533.3912220/94‑1 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCAGAAGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              <  SRR4302533.4754502/94‑1 (MQ=60)
                                                  TGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATT                                             >  SRR4302533.3432180/1‑94 (MQ=60)
                                                    GCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCC                                           <  SRR4302533.4097655/94‑1 (MQ=60)
                                                     CGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCG                                          >  SRR4302533.4250161/1‑94 (MQ=60)
                                                     CGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCG                                          >  SRR4302533.3072310/1‑94 (MQ=60)
                                                      GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGGTAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTTCGT                                         >  SRR4302533.5627938/1‑94 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCTCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        <  SRR4302533.5407161/94‑1 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGCCGGTTACTGTTAAACTTGGCTAATTttttccgttccgccctgggtttcgcc                                        >  SRR4302533.1127729/1‑68 (MQ=54)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302533.2720292/1‑94 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302533.2216904/1‑94 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302533.5496154/1‑94 (MQ=60)
                                                       CGCTTCCCGACACAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        >  SRR4302533.123418/1‑94 (MQ=60)
                                                        GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT                                       <  SRR4302533.4654132/94‑1 (MQ=60)
                                                          TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT                                     <  SRR4302533.4261656/94‑1 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    <  SRR4302533.866012/94‑1 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    <  SRR4302533.1540640/94‑1 (MQ=60)
                                                            CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   <  SRR4302533.4635778/94‑1 (MQ=60)
                                                            CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   <  SRR4302533.1472686/94‑1 (MQ=60)
                                                             CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA                                  >  SRR4302533.2145529/1‑94 (MQ=60)
                                                             CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA                                  <  SRR4302533.3452230/94‑1 (MQ=60)
                                                              CGACGCAGGCGCGTCCCTTCGCAGCTGGCTCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT                                 <  SRR4302533.1054111/94‑1 (MQ=60)
                                                              CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT                                 <  SRR4302533.2611833/94‑1 (MQ=60)
                                                               GACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATA                                <  SRR4302533.3749984/94‑1 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302533.5084481/94‑1 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302533.6197234/94‑1 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               >  SRR4302533.3428380/1‑94 (MQ=60)
                                                                 CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC                              <  SRR4302533.4268014/94‑1 (MQ=60)
                                                                 CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC                              <  SRR4302533.4470270/94‑1 (MQ=60)
                                                                  GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG                             <  SRR4302533.6181673/94‑1 (MQ=60)
                                                                  GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG                             >  SRR4302533.837861/1‑94 (MQ=60)
                                                                  GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG                             <  SRR4302533.3444364/94‑1 (MQ=60)
                                                                   CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG                            <  SRR4302533.2582563/94‑1 (MQ=60)
                                                                   CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG                            >  SRR4302533.1629472/1‑94 (MQ=60)
                                                                   CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG                            <  SRR4302533.3936261/94‑1 (MQ=60)
                                                                    AGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGatacagcagatcgga                           >  SRR4302533.2890121/1‑79 (MQ=60)
                                                                    AGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC                           >  SRR4302533.5708448/1‑94 (MQ=60)
                                                                     GGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCG                          <  SRR4302533.2744785/94‑1 (MQ=60)
                                                                       CGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCT                        >  SRR4302533.156054/1‑94 (MQ=60)
                                                                       CGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCT                        <  SRR4302533.4017727/94‑1 (MQ=60)
                                                                         CGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCC                      <  SRR4302533.3035685/94‑1 (MQ=60)
                                                                         AGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGAGCTCC                      >  SRR4302533.3136496/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCTGCGCTCCA                     >  SRR4302533.5494511/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302533.1708518/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302533.4262815/1‑94 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  <  SRR4302533.473643/94‑1 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  <  SRR4302533.243109/94‑1 (MQ=60)
                                                                               TTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTA                >  SRR4302533.5123352/1‑94 (MQ=60)
                                                                                 CGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATG              <  SRR4302533.2445217/94‑1 (MQ=60)
                                                                                 CGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATG              <  SRR4302533.4259465/94‑1 (MQ=60)
                                                                                  GCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGC             <  SRR4302533.738647/94‑1 (MQ=60)
                                                                                  GCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGC             >  SRR4302533.5771390/1‑94 (MQ=60)
                                                                                   CAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCATCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCA            >  SRR4302533.1752119/1‑94 (MQ=60)
                                                                                    AGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAG           >  SRR4302533.5014122/1‑94 (MQ=60)
                                                                                     GCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGC          >  SRR4302533.4891067/1‑94 (MQ=60)
                                                                                      CTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCA         >  SRR4302533.2427529/1‑94 (MQ=60)
                                                                                      CTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCA         <  SRR4302533.886389/94‑1 (MQ=60)
                                                                                       TGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCAC        >  SRR4302533.5527849/1‑94 (MQ=60)
                                                                                          CGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCC     <  SRR4302533.5619086/94‑1 (MQ=60)
                                                                                          CGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCC     >  SRR4302533.4343641/1‑94 (MQ=60)
                                                                                           GCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCT    <  SRR4302533.5891756/94‑1 (MQ=60)
                                                                                             GCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  <  SRR4302533.2000394/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270142‑4270328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑