Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,284,295 A→G V219A (GTA→GCA)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,284,2950AG100.0% 665.7 / NA 205V219A (GTA→GCA) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (111/94);  total (111/94)

Only 100 of 215 total aligned reads displayed.

TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384
                                                                                          |                                                                                         
tGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgcc                                                                                         >  1:5817096/1‑93 (MQ=255)
tGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgcc                                                                                         >  2:3606954/1‑93 (MQ=255)
 ggTGCTGATCGCTCGCTTGCTCACCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgcca                                                                                        <  2:2567729/93‑1 (MQ=255)
  gTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgccac                                                                                       >  1:1120398/1‑93 (MQ=255)
   tGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgccacc                                                                                      >  2:4647797/1‑93 (MQ=255)
    gCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGccacca                                                                                     >  2:5974093/1‑93 (MQ=255)
    gCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGccacca                                                                                     >  1:1486096/1‑93 (MQ=255)
     cTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCcaccac                                                                                    <  1:5211474/93‑1 (MQ=255)
      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  1:4369959/1‑93 (MQ=255)
      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  1:2166464/1‑93 (MQ=255)
      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  2:2408468/1‑93 (MQ=255)
       gATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACAt                                                                                  <  1:4765629/93‑1 (MQ=255)
       gATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACAt                                                                                  >  1:1642574/1‑93 (MQ=255)
        aTCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATg                                                                                 <  1:3781374/93‑1 (MQ=255)
           cctcgctTGCTCAACCAGGTCGTCCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGctc                                                                              <  1:4811406/92‑1 (MQ=255)
            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCTACCACATGctct                                                                             <  2:1873991/93‑1 (MQ=255)
            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctct                                                                             >  2:3000804/1‑93 (MQ=255)
            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctct                                                                             <  1:6288041/93‑1 (MQ=255)
            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctct                                                                             >  1:5969619/1‑93 (MQ=255)
              cgctTGCTCACCCAGGTCGTCCCACCACAGGTCGCCCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTAc                                                                           <  2:796834/93‑1 (MQ=255)
              cgctTCCCCAACCAGGGCGTCCCCCCACGGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTAc                                                                           <  1:4572101/93‑1 (MQ=255)
              cgctGGCTCACCCAGGTCGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTAc                                                                           <  2:733101/93‑1 (MQ=255)
               gctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACg                                                                          >  1:4636473/1‑93 (MQ=255)
               actTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACg                                                                          >  1:5413957/2‑93 (MQ=255)
                ctTGCTCAACCAGTGCGTCCCACCACAGGGCGCCCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGc                                                                         <  2:3058654/93‑1 (MQ=255)
                 ttGCTCACCCAGGTCGTCCCACCACAGGTCCCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCt                                                                        <  2:458345/93‑1 (MQ=255)
                 ttGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCt                                                                        >  2:1301387/1‑93 (MQ=255)
                  tccTCAACCAGGTCGTCCCACCACAGGTCGCCCCCTTCCTGCCAGTAAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTg                                                                       <  2:5299178/91‑1 (MQ=255)
                  tGCTCAACCAGGTCGTGCCCCCACAGGTCGCCCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTg                                                                       <  2:5529953/93‑1 (MQ=255)
                  tGCCCAACCAGGTCGTGCCCCCACAGGTCGCCCCTTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTg                                                                       <  2:5015692/93‑1 (MQ=255)
                        aCCAGGTCGTGCCCCCCCAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACg                                                                 <  2:1749524/93‑1 (MQ=255)
                        aCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACg                                                                 >  2:2850430/1‑93 (MQ=255)
                          cAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               >  2:131577/1‑93 (MQ=255)
                           aGGTCGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTc                                                              <  1:2495378/93‑1 (MQ=255)
                           aGGTCGTCCCACCACAGGTCGCCCCTTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTc                                                              <  2:1400119/93‑1 (MQ=255)
                               cgcgCCACCACAGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGt                                                          <  2:6264124/90‑1 (MQ=255)
                                gTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         >  1:5607587/1‑93 (MQ=255)
                                gTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         >  1:1656803/1‑93 (MQ=255)
                                 tGCCACCACAGGTCGCGCCTTGCCGGCCAGTCCATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGttt                                                        <  1:2565628/93‑1 (MQ=255)
                                 tGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGttt                                                        >  1:3605907/1‑93 (MQ=255)
                                 tGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGgtt                                                        >  2:2071818/1‑93 (MQ=255)
                                  cccaccaCAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtttt                                                       <  2:3419572/92‑1 (MQ=255)
                                    caccacAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTc                                                     >  1:399434/1‑93 (MQ=255)
                                     cccacAGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCa                                                    <  2:2665350/92‑1 (MQ=255)
                                     accacAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCa                                                    >  1:5451847/1‑93 (MQ=255)
                                       cacaGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgc                                                  >  1:958918/1‑93 (MQ=255)
                                       cacaGGTCGCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgc                                                  <  2:5791639/93‑1 (MQ=255)
                                        ccaGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                 <  2:3162151/92‑1 (MQ=255)
                                         caGGTCCCCCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcgc                                                <  2:5884094/93‑1 (MQ=255)
                                           ggTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGt                                              >  1:318476/1‑93 (MQ=255)
                                           ggTCGCGCCCTCCCTGCCAGTCAATTTGCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGt                                              <  1:293699/93‑1 (MQ=255)
                                            gTCCCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtc                                             <  2:1522467/93‑1 (MQ=255)
                                             tCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtca                                            >  2:1215504/1‑93 (MQ=255)
                                             gcgcgCCTTTCCTGCCAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtca                                            <  1:416589/92‑1 (MQ=255)
                                                cgcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatca                                         >  2:3403763/1‑93 (MQ=255)
                                                cccccTTCCTCCCATTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatca                                         <  1:4083109/91‑1 (MQ=255)
                                                 gcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        >  2:6171715/1‑93 (MQ=255)
                                                  ccctccctGCAAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                       <  2:37919/93‑1 (MQ=255)
                                                  cccTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                       >  2:5055101/1‑93 (MQ=255)
                                                    cTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGt                                     >  1:2673968/1‑93 (MQ=255)
                                                     ttCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    >  2:4841926/1‑93 (MQ=255)
                                                     ttCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    <  1:1320340/93‑1 (MQ=255)
                                                      tCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGttt                                   >  1:1205246/1‑93 (MQ=255)
                                                       ccTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtttt                                  >  2:396912/1‑93 (MQ=255)
                                                        cTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGttttt                                 <  2:537827/93‑1 (MQ=255)
                                                         tGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttc                                >  2:853430/1‑93 (MQ=255)
                                                         tGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttc                                <  1:1551417/93‑1 (MQ=255)
                                                         ccccAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttc                                <  2:2226537/91‑1 (MQ=255)
                                                          gCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttct                               >  2:4194855/1‑93 (MQ=255)
                                                           ccAGCCAATTTGCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                              <  2:6074468/93‑1 (MQ=255)
                                                            cAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             <  2:5278757/93‑1 (MQ=255)
                                                             aGTCAATTTGCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            <  1:3416369/93‑1 (MQ=255)
                                                             aGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            <  2:5581028/93‑1 (MQ=255)
                                                              gTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           >  2:2783052/1‑93 (MQ=255)
                                                              gTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           >  2:2390603/1‑93 (MQ=255)
                                                                cAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCg                         <  1:4118699/93‑1 (MQ=255)
                                                                 aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        >  1:103931/1‑93 (MQ=255)
                                                                 aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGCTTTTCAGCGCGTCATCAACGCTTTTCTTCAGCgg                        >  2:2902388/1‑93 (MQ=255)
                                                                  aTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       >  2:3837738/1‑93 (MQ=255)
                                                                    tttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAAt                     <  1:4778067/93‑1 (MQ=255)
                                                                    ttccccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAAt                     <  2:3319662/90‑1 (MQ=255)
                                                                        ccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTg                 <  2:4048266/93‑1 (MQ=255)
                                                                          gCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgcg               <  1:1388923/93‑1 (MQ=255)
                                                                           ccAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgcgc              >  2:294477/1‑93 (MQ=255)
                                                                            cAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc             >  2:2315895/1‑93 (MQ=255)
                                                                             aGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGccc            >  1:788112/1‑93 (MQ=255)
                                                                              gTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCg           >  2:1591844/1‑93 (MQ=255)
                                                                               tACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCgg          >  2:1831726/1‑93 (MQ=255)
                                                                                 cGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgca        <  1:4869733/93‑1 (MQ=255)
                                                                                  gCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcac       >  2:5414199/1‑93 (MQ=255)
                                                                                  gCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcac       <  1:5344599/93‑1 (MQ=255)
                                                                                  gCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcac       >  1:4350457/1‑93 (MQ=255)
                                                                                   cTTCAGTGCCACCACATGCTCTACGCTGGTGACGGTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacg      >  2:645068/1‑93 (MQ=255)
                                                                                     tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    <  2:4439009/93‑1 (MQ=255)
                                                                                     tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    <  1:3055041/93‑1 (MQ=255)
                                                                                     tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    >  1:3552329/1‑93 (MQ=255)
                                                                                      cAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgcac   <  2:518520/93‑1 (MQ=255)
                                                                                       aGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGcaca  >  1:1816303/1‑93 (MQ=255)
                                                                                       aGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGcaca  <  2:2481050/93‑1 (MQ=255)
                                                                                       aGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGcaca  <  1:502024/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACAC  >  NC_000913/4284202‑4284385
                                                                                             |                                                                                          
cccgggtcctgaccgctcgctgcctcacCCAGGTCGTGCCCCCACAGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTG                                                                                            <  SRR4302533.5545961/66‑1 (MQ=60)
 CCTGGTGCTGATCGCTCGCTTGCTCACCCAGGTCGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGC                                                                                           <  SRR4302533.5263654/94‑1 (MQ=60)
 CCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGC                                                                                           >  SRR4302533.6240170/1‑94 (MQ=60)
  CTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCC                                                                                          >  SRR4302533.5852237/1‑94 (MQ=60)
   TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCA                                                                                         >  SRR4302533.4385394/1‑94 (MQ=60)
   TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCA                                                                                         >  SRR4302533.2328275/1‑94 (MQ=60)
    GGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCAC                                                                                        >  SRR4302533.1127045/1‑94 (MQ=60)
     GTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACC                                                                                       >  SRR4302533.4632306/1‑94 (MQ=60)
      TGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCA                                                                                      >  SRR4302533.1495063/1‑94 (MQ=60)
       GCTGATCGCTCGCTTGCTCAACCAGGTCGTCCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCCCCAC                                                                                     <  SRR4302533.2006799/94‑1 (MQ=60)
        CTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACA                                                                                    >  SRR4302533.2179790/1‑94 (MQ=60)
        CTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACA                                                                                    >  SRR4302533.4396342/1‑94 (MQ=60)
        CTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACA                                                                                    <  SRR4302533.5242860/94‑1 (MQ=60)
         TGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACAT                                                                                   >  SRR4302533.1652522/1‑94 (MQ=60)
          TATCGCTCGTTTCCTCAACCAGGTCGTCCCACCACAGGTCGCGCCCTTCCTGCCGGTAAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATG                                                                                  <  SRR4302533.4887140/94‑1 (MQ=60)
          GATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATG                                                                                  <  SRR4302533.4794527/94‑1 (MQ=60)
          GATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATG                                                                                  >  SRR4302533.1684243/1‑94 (MQ=60)
           ATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGC                                                                                 <  SRR4302533.3804535/94‑1 (MQ=60)
           ATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGC                                                                                 >  SRR4302533.4956147/1‑94 (MQ=60)
            TCGCTCGCTTCCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCT                                                                                <  SRR4302533.5830535/94‑1 (MQ=60)
             CGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTC                                                                               >  SRR4302533.4586800/1‑94 (MQ=60)
              GCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCT                                                                              >  SRR4302533.6005755/1‑94 (MQ=60)
              CCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCT                                                                              <  SRR4302533.4840565/94‑1 (MQ=60)
              CCTCGCTTGCTCAACCAGGTCGTCCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCT                                                                              <  SRR4302533.4840566/94‑1 (MQ=60)
               CTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTA                                                                             <  SRR4302533.6325552/94‑1 (MQ=60)
                TCGCTTGCTCACCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTAC                                                                            <  SRR4302533.3983832/94‑1 (MQ=60)
                 CGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACG                                                                           >  SRR4302533.4664568/1‑94 (MQ=60)
                 CGCTTCCCCAACCAGGGCGTCCCCCCACGGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACG                                                                           <  SRR4302533.4599808/94‑1 (MQ=60)
                 CACTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACG                                                                           >  SRR4302533.5446513/1‑94 (MQ=60)
                   CTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCT                                                                         >  SRR4302533.2939095/1‑94 (MQ=60)
                   CTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCT                                                                         <  SRR4302533.3451796/94‑1 (MQ=60)
                       CTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTG                                                                     >  SRR4302533.1386204/1‑94 (MQ=60)
                        TCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGA                                                                    >  SRR4302533.598912/1‑94 (MQ=60)
                         CACCCAGGTCGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGAC                                                                   <  SRR4302533.5106630/94‑1 (MQ=60)
                           ACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGT                                                                 >  SRR4302533.4796095/1‑94 (MQ=60)
                             CAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGGTC                                                               >  SRR4302533.2888217/1‑94 (MQ=60)
                              AGGTCGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCG                                                              <  SRR4302533.2510771/94‑1 (MQ=60)
                                GTCGCGCCACCACGGGTCGCGCCTTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGG                                                            <  SRR4302533.2961079/94‑1 (MQ=60)
                                 TCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGT                                                           >  SRR4302533.1887681/1‑94 (MQ=60)
                                 TCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGT                                                           >  SRR4302533.4126420/1‑94 (MQ=60)
                                  CGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTT                                                          >  SRR4302533.1666830/1‑94 (MQ=60)
                                  CGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTT                                                          >  SRR4302533.5641471/1‑94 (MQ=60)
                                   GTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTT                                                         >  SRR4302533.3628101/1‑94 (MQ=60)
                                    TGCCACCACAGGTCGCGCCTTGCCGGCCAGTCCATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTT                                                        <  SRR4302533.2581416/94‑1 (MQ=60)
                                      CCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTC                                                      >  SRR4302533.401846/1‑94 (MQ=60)
                                       CACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCA                                                     >  SRR4302533.5484688/1‑94 (MQ=60)
                                       CACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCA                                                     >  SRR4302533.5223355/1‑94 (MQ=60)
                                       CACCAAAGGCCGCGCCCTCGCTGGCAGTCAATTCTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCA                                                     <  SRR4302533.1169268/94‑1 (MQ=60)
                                        CCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAG                                                    <  SRR4302533.1632112/94‑1 (MQ=60)
                                         CCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGC                                                   >  SRR4302533.964557/1‑94 (MQ=60)
                                            CAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCG                                                >  SRR4302533.5404436/1‑94 (MQ=60)
                                             AGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGT                                               >  SRR4302533.320428/1‑94 (MQ=60)
                                              GGTCGCGCCCTCCCTGCCAGTCAATTTGCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTC                                              <  SRR4302533.295492/94‑1 (MQ=60)
                                                tcgtgcctgtcctgcatGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCAT                                            <  SRR4302533.777951/77‑1 (MQ=60)
                                                GCGCGCCTTTCCTGCCAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCAT                                            <  SRR4302533.419091/94‑1 (MQ=60)
                                                  GCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCA                                          >  SRR4302533.1314657/1‑94 (MQ=60)
                                                   CCCCCTTCCTCCCATTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAA                                         <  SRR4302533.4107964/94‑1 (MQ=60)
                                                    GCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGCGGACGTTCGGGTTTTTCAGCGCGTCATCAAG                                        >  SRR4302533.4160906/1‑94 (MQ=60)
                                                      CCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGT                                      >  SRR4302533.2690369/1‑94 (MQ=60)
                                                       CTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTT                                     >  SRR4302533.1666768/1‑94 (MQ=60)
                                                        TTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTT                                    >  SRR4302533.2589310/1‑94 (MQ=60)
                                                        TTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTT                                    <  SRR4302533.1328267/94‑1 (MQ=60)
                                                        TTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTT                                    >  SRR4302533.1212489/1‑94 (MQ=60)
                                                           CTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTC                                 <  SRR4302533.2521241/94‑1 (MQ=60)
                                                            TGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCT                                <  SRR4302533.1560760/94‑1 (MQ=60)
                                                              CCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTC                              >  SRR4302533.3776892/1‑94 (MQ=60)
                                                               CAGTAATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCA                             <  SRR4302533.5871681/94‑1 (MQ=60)
                                                                AGTCAATTTGCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAG                            <  SRR4302533.3437476/94‑1 (MQ=60)
                                                                 GTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGC                           >  SRR4302533.3159101/1‑94 (MQ=60)
                                                                   CAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGG                         >  SRR4302533.5475366/1‑94 (MQ=60)
                                                                   CAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGG                         <  SRR4302533.4044235/94‑1 (MQ=60)
                                                                   CAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGG                         >  SRR4302533.62505/1‑94 (MQ=60)
                                                                   CAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGG                         >  SRR4302533.104582/1‑94 (MQ=60)
                                                                   CAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGG                         >  SRR4302533.5302656/1‑94 (MQ=60)
                                                                   CAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGG                         <  SRR4302533.4143714/94‑1 (MQ=60)
                                                                       TTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATA                     <  SRR4302533.4807009/94‑1 (MQ=60)
                                                                       TTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATA                     <  SRR4302533.72058/94‑1 (MQ=60)
                                                                        TTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATAC                    >  SRR4302533.360339/1‑94 (MQ=60)
                                                                        TCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATAC                    <  SRR4302533.338386/94‑1 (MQ=60)
                                                                          CCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTG                  <  SRR4302533.1069233/94‑1 (MQ=60)
                                                                            CGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCG                <  SRR4302533.1644723/94‑1 (MQ=60)
                                                                             GCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGC               <  SRR4302533.1397288/94‑1 (MQ=60)
                                                                               CAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCC             >  SRR4302533.792743/1‑94 (MQ=60)
                                                                               CAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCC             <  SRR4302533.4754473/94‑1 (MQ=60)
                                                                                AGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCG            >  SRR4302533.1480896/1‑94 (MQ=60)
                                                                                AGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGCTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCG            >  SRR4302533.962759/1‑94 (MQ=60)
                                                                                 GTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGG           <  SRR4302533.3732500/94‑1 (MQ=60)
                                                                                  TACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGC          >  SRR4302533.5545238/1‑94 (MQ=60)
                                                                                  TACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGC          <  SRR4302533.1547932/94‑1 (MQ=60)
                                                                                    CGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCAC        <  SRR4302533.458453/94‑1 (MQ=60)
                                                                                    CGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCAC        <  SRR4302533.4899240/94‑1 (MQ=60)
                                                                                    CGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCAC        >  SRR4302533.4376710/1‑94 (MQ=60)
                                                                                     GCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACG       <  SRR4302533.5376653/94‑1 (MQ=60)
                                                                                       TTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCA     >  SRR4302533.3574209/1‑94 (MQ=60)
                                                                                        TCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCAC    <  SRR4302533.3073767/94‑1 (MQ=60)
                                                                                         CAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA   >  SRR4302533.1827391/1‑94 (MQ=60)
                                                                                         CAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA   <  SRR4302533.4281246/94‑1 (MQ=60)
                                                                                          AGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACAC  <  SRR4302533.505009/94‑1 (MQ=60)
                                                                                          AGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACAC  >  SRR4302533.1187164/1‑94 (MQ=60)
                                                                                             |                                                                                          
GCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACAC  >  NC_000913/4284202‑4284385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑