Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,315,907 +A coding (436/759 nt) phnK ← ATP‑binding component of phosphonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,315,9031.A97.8% 852.9 / 7.6 233coding (440/759 nt)phnKATP‑binding component of phosphonate transport
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base A (121/106);  total (124/109)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 263 total aligned reads displayed.

CATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑AAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGT  >  NC_000913/4315811‑4315990
                                                                                              |                                                                                       
   ccAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑aaa                                                                                      >  1:5897521/1‑93 (MQ=255)
      gCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑aaaaag                                                                                   <  1:4599257/93‑3 (MQ=255)
      gCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑aaaaag                                                                                   >  2:3576526/1‑91 (MQ=255)
      ccccGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑aaaaag                                                                                   <  2:4478467/92‑3 (MQ=255)
        ccgcggGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCATACGCTGCTGCATACCGCCG‑G‑aaaaaggt                                                                                 <  2:4016947/93‑5 (MQ=255)
        ccgccgGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑aaaaaggt                                                                                 >  1:1356786/1‑89 (MQ=255)
        ccgccgGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑aaaaaggt                                                                                 <  2:4553020/93‑5 (MQ=255)
          gccgGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAggtgg                                                                                <  2:3852899/93‑1 (MQ=255)
          gccgGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAggtgg                                                                                >  2:2165300/1‑93 (MQ=255)
          gccgGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAggtgg                                                                                >  1:2487432/1‑93 (MQ=255)
            cggtcggtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTc                                                                              <  1:1196694/93‑1 (MQ=255)
            cggtcggtTCATCCATAAACACCAGCTACGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTc                                                                              <  2:2958203/93‑1 (MQ=255)
             ggtcggtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCg                                                                             <  2:5717367/93‑1 (MQ=255)
              gtcggtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCgg                                                                            >  2:5495748/1‑93 (MQ=255)
               tcggtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGc                                                                           <  2:4121723/93‑1 (MQ=255)
               tcggtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGc                                                                           <  2:4265324/93‑1 (MQ=255)
                cggtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCa                                                                          >  1:5190046/1‑93 (MQ=255)
                  gtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAgg                                                                        >  2:72104/1‑93 (MQ=255)
                  gtTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAgg                                                                        <  2:1776600/93‑1 (MQ=255)
                   ttCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAATGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGgt                                                                       >  2:1453066/1‑93 (MQ=255)
                   ttCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGGGGTCGGCAGgt                                                                       >  2:4394414/1‑93 (MQ=255)
                      aTCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGgtcgt                                                                    <  2:3760471/93‑1 (MQ=255)
                      aTCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGgtcgt                                                                    <  1:5799148/93‑1 (MQ=255)
                       tCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGgtcgtc                                                                   <  2:860995/93‑1 (MQ=255)
                       tCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGgtcgtc                                                                   >  1:3585640/1‑93 (MQ=255)
                       tCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGgtcgtc                                                                   <  2:2563403/93‑1 (MQ=255)
                         cATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GTAAAAGGTGGTCGGCAGGTCGTCGa                                                                 >  2:328477/1‑93 (MQ=255)
                           tAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATc                                                               <  2:9384/93‑1 (MQ=255)
                           tAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATc                                                               >  1:4528794/1‑93 (MQ=255)
                            aaaCACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATcc                                                              <  2:3191438/93‑1 (MQ=255)
                                acCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGtt                                                          >  2:1838499/1‑93 (MQ=255)
                                  cAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTgg                                                        <  2:5620518/93‑1 (MQ=255)
                                  cAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTgg                                                        >  1:913801/1‑93 (MQ=255)
                                    gCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCg                                                      <  1:1741284/93‑1 (MQ=255)
                                         ggATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAAt                                                 <  2:3217556/93‑1 (MQ=255)
                                          gATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAAtc                                                >  2:816477/1‑93 (MQ=255)
                                          gATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAAtc                                                >  2:3377592/1‑93 (MQ=255)
                                            tGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAAtctc                                              <  1:4407030/93‑1 (MQ=255)
                                            tGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAAtctc                                              <  2:1410564/93‑1 (MQ=255)
                                             gCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTcc                                             <  2:2745641/93‑1 (MQ=255)
                                              cGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCa                                            <  2:4839293/93‑1 (MQ=255)
                                              cGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCa                                            <  1:2335142/93‑1 (MQ=255)
                                                tCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCAcc                                          >  2:5942019/1‑93 (MQ=255)
                                                tCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCAcc                                          >  1:3441642/1‑93 (MQ=255)
                                                  aCCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACctc                                        >  2:1139005/1‑93 (MQ=255)
                                                  aCCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACctc                                        <  2:6274325/93‑1 (MQ=255)
                                                   ccAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACctct                                       <  2:3474166/93‑1 (MQ=255)
                                                      ggTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTcc                                    >  1:2543116/1‑93 (MQ=255)
                                                      ggTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTcc                                    >  1:5633417/1‑93 (MQ=255)
                                                      ggTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTcc                                    >  1:5564038/1‑93 (MQ=255)
                                                      ggTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTcc                                    >  2:5656523/1‑93 (MQ=255)
                                                       gTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCa                                   <  1:4172658/93‑1 (MQ=255)
                                                       gTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCa                                   >  1:1641103/1‑93 (MQ=255)
                                                       gTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCa                                   >  1:3340238/1‑93 (MQ=255)
                                                        ttGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAg                                  >  1:1067447/1‑93 (MQ=255)
                                                        ttGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAg                                  >  2:1063704/1‑93 (MQ=255)
                                                           cGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCa                               >  2:2379680/1‑93 (MQ=255)
                                                           cGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCa                               <  1:1977057/93‑1 (MQ=255)
                                                            gggCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCCCCTCTTCCAGCCAc                              <  1:2862543/93‑1 (MQ=255)
                                                            gggCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCAc                              >  2:2983157/1‑93 (MQ=255)
                                                             ggCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACt                             >  1:2236076/1‑93 (MQ=255)
                                                             ggCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACt                             >  1:4312009/1‑93 (MQ=255)
                                                             ggCAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGGTGGCGGGAATCTCCACCTCTTCCAGCCACt                             >  1:5061168/1‑93 (MQ=255)
                                                               cAATCTGCAAACGCTGCTGCATCCCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTc                           <  1:3918484/93‑1 (MQ=255)
                                                               cAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTc                           >  1:109394/1‑93 (MQ=255)
                                                               cAATCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTc                           <  2:2491755/93‑1 (MQ=255)
                                                                aaTCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCt                          <  1:953653/93‑1 (MQ=255)
                                                                 aTCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTg                         <  2:1294620/93‑1 (MQ=255)
                                                                  tCTGCAACCGCTGCTGCATCCCGCGG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGAAATCCCCCCCTCTCCCAGCCACTTCTgc                        <  2:4992982/93‑1 (MQ=255)
                                                                  tCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTgc                        >  2:5332427/1‑93 (MQ=255)
                                                                  tCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTgc                        <  1:6109469/93‑1 (MQ=255)
                                                                  tCTGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTgc                        <  2:2455268/93‑1 (MQ=255)
                                                                    tGCAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTgcgc                      >  1:500183/1‑93 (MQ=255)
                                                                      cAAACGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCgg                    <  2:2841388/93‑1 (MQ=255)
                                                                      cAAACGCGGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCgg                    <  1:449684/93‑1 (MQ=255)
                                                                       aaaCGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGGCACTTCTGCGCggg                   >  2:5050203/1‑92 (MQ=255)
                                                                         aCGCTGCTGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTgg                 <  1:4090771/93‑1 (MQ=255)
                                                                           gctgctGCATACCGCCGAA‑AAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCa               <  2:6041036/93‑1 (MQ=255)
                                                                           gctgctGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCa               <  2:6178620/93‑1 (MQ=255)
                                                                           gctgctGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCa               <  2:5151395/93‑1 (MQ=255)
                                                                           gctgctGCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCa               <  2:4072515/93‑1 (MQ=255)
                                                                            ctgctgCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCAc              <  1:3958614/93‑1 (MQ=255)
                                                                            ctgctgCATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCAc              >  1:2628800/1‑93 (MQ=255)
                                                                             tgctgcATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACg             <  1:6193535/93‑1 (MQ=255)
                                                                             tgctgcATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACg             >  1:3252707/1‑93 (MQ=255)
                                                                             tgctgcATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCAACCTCTTCCAGCCACTTCTGCGCGGTGGCACg             >  1:1928581/1‑93 (MQ=255)
                                                                               ctgcATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGaa           <  2:5825377/93‑1 (MQ=255)
                                                                                tgcATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAat          >  2:1580291/1‑93 (MQ=255)
                                                                                tgcATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGCGGCACGAat          >  1:4923576/1‑93 (MQ=255)
                                                                                 gcATGCCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCTATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAata         <  1:5532919/93‑1 (MQ=255)
                                                                                 gcATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAata         <  2:2070110/93‑1 (MQ=255)
                                                                                  cATACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAatat        <  2:1250131/93‑1 (MQ=255)
                                                                                   aTACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATc       >  1:2994039/1‑93 (MQ=255)
                                                                                   aTACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATc       >  1:4645889/1‑93 (MQ=255)
                                                                                   aTACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATc       <  1:759944/93‑1 (MQ=255)
                                                                                    tACCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCg      >  2:169869/1‑93 (MQ=255)
                                                                                     aCCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGc     >  1:1627/1‑93 (MQ=255)
                                                                                     aCCGCCG‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGc     <  1:1532433/93‑1 (MQ=255)
                                                                                      ccgccg‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGcc    >  2:4887792/1‑93 (MQ=255)
                                                                                        gccg‑GAAAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGt  <  2:1636942/93‑1 (MQ=255)
                                                                                              |                                                                                       
CATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCG‑G‑AAAAGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGT  >  NC_000913/4315811‑4315990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGACGTGC  >  NC_000913/4315814‑4316000
                                                                                               |                                                                                              
CCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑A                                                                                                <  SRR4302533.4632544/94‑1 (MQ=60)
 CAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑A‑A                                                                                              <  SRR4302533.3554005/94‑1 (MQ=60)
 CAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑A‑A                                                                                              <  SRR4302533.6018179/94‑1 (MQ=60)
   GCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑Aagg                                                                                             <  SRR4302533.4627121/94‑4 (MQ=60)
   GCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑Aagg                                                                                             <  SRR4302533.3991221/94‑4 (MQ=60)
   GCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑Aagg                                                                                             >  SRR4302533.4147271/1‑91 (MQ=60)
    CCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGT                                                                                            <  SRR4302533.4616380/94‑1 (MQ=60)
    CCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGT                                                                                            >  SRR4302533.1364939/1‑94 (MQ=60)
     CCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTG                                                                                           <  SRR4302533.4363236/94‑1 (MQ=60)
     CCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTG                                                                                           >  SRR4302533.5565756/1‑94 (MQ=60)
     CCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAACA‑GGTG                                                                                           >  SRR4302533.4182503/1‑94 (MQ=60)
      CGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGG                                                                                          >  SRR4302533.2502753/1‑94 (MQ=60)
      CGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGG                                                                                          <  SRR4302533.238699/94‑1 (MQ=60)
        CCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTC                                                                                        <  SRR4302533.2881157/94‑1 (MQ=60)
         CGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCG                                                                                       <  SRR4302533.1203884/94‑1 (MQ=60)
         CGATCGGTTCATCCATAAACACCAGCTTCGGATGCGTAACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCG                                                                                       <  SRR4302533.4985614/94‑1 (MQ=60)
           GTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGC                                                                                     >  SRR4302533.3712635/1‑94 (MQ=60)
            TCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCA                                                                                    <  SRR4302533.5761002/94‑1 (MQ=60)
            TCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCA                                                                                    >  SRR4302533.5221361/1‑94 (MQ=60)
              GGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGG                                                                                  <  SRR4302533.4869225/94‑1 (MQ=60)
              GGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGG                                                                                  >  SRR4302533.1941959/1‑94 (MQ=60)
                TTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTC                                                                                <  SRR4302533.4957478/94‑1 (MQ=60)
                 TCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCG                                                                               <  SRR4302533.2339780/94‑1 (MQ=60)
                 TCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCG                                                                               <  SRR4302533.1118904/94‑1 (MQ=60)
                  CATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGT                                                                              >  SRR4302533.5614014/1‑94 (MQ=60)
                   ATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTC                                                                             >  SRR4302533.1904419/1‑94 (MQ=60)
                   ATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTC                                                                             >  SRR4302533.2545391/1‑94 (MQ=60)
                   ATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTC                                                                             >  SRR4302533.3607723/1‑94 (MQ=60)
                   ATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTC                                                                             <  SRR4302533.5834184/94‑1 (MQ=60)
                   ATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTC                                                                             >  SRR4302533.558041/1‑94 (MQ=60)
                   ATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTC                                                                             <  SRR4302533.5058585/94‑1 (MQ=60)
                    TCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCG                                                                            <  SRR4302533.4560137/94‑1 (MQ=60)
                     CCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGA                                                                           >  SRR4302533.399659/1‑94 (MQ=60)
                     CCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGGCGTCGA                                                                           >  SRR4302533.4543121/1‑94 (MQ=60)
                      CATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGAT                                                                          <  SRR4302533.1742925/94‑1 (MQ=60)
                      CATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGAT                                                                          >  SRR4302533.2177716/1‑94 (MQ=60)
                      CATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGAT                                                                          <  SRR4302533.770467/94‑1 (MQ=60)
                       ATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATC                                                                         >  SRR4302533.4556220/1‑94 (MQ=60)
                       ATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATC                                                                         >  SRR4302533.3360538/1‑94 (MQ=60)
                        TAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCC                                                                        <  SRR4302533.6153414/94‑1 (MQ=60)
                            CACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTT                                                                    <  SRR4302533.2818961/94‑1 (MQ=60)
                              CCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGG                                                                  <  SRR4302533.1637258/94‑1 (MQ=60)
                              CCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGG                                                                  >  SRR4302533.561757/1‑94 (MQ=60)
                              CCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGG                                                                  >  SRR4302533.919172/1‑94 (MQ=60)
                               CAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGC                                                                 >  SRR4302533.931107/1‑94 (MQ=60)
                                 GCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGG                                                               <  SRR4302533.1751842/94‑1 (MQ=60)
                                 GCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGG                                                               <  SRR4302533.1091237/94‑1 (MQ=60)
                                  CTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGG                                                              >  SRR4302533.2502054/1‑94 (MQ=60)
                                     CGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAAT                                                           >  SRR4302533.983676/1‑94 (MQ=60)
                                      GGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATC                                                          >  SRR4302533.165749/1‑94 (MQ=60)
                                        ATGCCGCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATCCCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTC                                                        <  SRR4302533.1804981/94‑1 (MQ=60)
                                         TGCGTCTCCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCC                                                       <  SRR4302533.2480823/94‑1 (MQ=60)
                                         TGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCC                                                       <  SRR4302533.4433641/94‑1 (MQ=60)
                                         TGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGCTTGGCGGGAATCTCC                                                       >  SRR4302533.4384335/1‑94 (MQ=60)
                                          GCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCA                                                      <  SRR4302533.1623668/94‑1 (MQ=60)
                                           CGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCAC                                                     <  SRR4302533.2349597/94‑1 (MQ=60)
                                           CGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCAC                                                     <  SRR4302533.2583919/94‑1 (MQ=60)
                                            GTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACC                                                    >  SRR4302533.3462896/1‑94 (MQ=60)
                                               ACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCT                                                 >  SRR4302533.5855636/1‑94 (MQ=60)
                                                  AGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCC                                              >  SRR4302533.5597668/1‑94 (MQ=60)
                                                  AGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCC                                              >  SRR4302533.5667483/1‑94 (MQ=60)
                                                  AGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCC                                              >  SRR4302533.2558765/1‑94 (MQ=60)
                                                   GGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCA                                             >  SRR4302533.1651045/1‑94 (MQ=60)
                                                   GGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCA                                             >  SRR4302533.3360883/1‑94 (MQ=60)
                                                    GTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAG                                            <  SRR4302533.4197944/94‑1 (MQ=60)
                                                    GTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAG                                            >  SRR4302533.1073726/1‑94 (MQ=60)
                                                        CGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCAC                                        <  SRR4302533.5893381/94‑1 (MQ=60)
                                                        CGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCAC                                        <  SRR4302533.1989166/94‑1 (MQ=60)
                                                         GGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCCCCTCTTCCAGCCACT                                       <  SRR4302533.2880153/94‑1 (MQ=60)
                                                         GGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACT                                       >  SRR4302533.2249900/1‑94 (MQ=60)
                                                         GGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACT                                       >  SRR4302533.4337968/1‑94 (MQ=60)
                                                         GGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACT                                       >  SRR4302533.5091754/1‑94 (MQ=60)
                                                         GGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGGTGGCGGGAATCTCCACCTCTTCCAGCCACT                                       >  SRR4302533.5091753/1‑94 (MQ=60)
                                                          GGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTT                                      <  SRR4302533.2838246/94‑1 (MQ=60)
                                                          GGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTT                                      >  SRR4302533.1862256/1‑94 (MQ=60)
                                                          GGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTT                                      >  SRR4302533.348259/1‑94 (MQ=60)
                                                           GCAATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTC                                     >  SRR4302533.110089/1‑94 (MQ=60)
                                                            CAATCTGCAAACGCTGCTGCATCCCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCT                                    <  SRR4302533.3942411/94‑1 (MQ=60)
                                                             AATCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTG                                   <  SRR4302533.959255/94‑1 (MQ=60)
                                                               TCTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCG                                 <  SRR4302533.6146350/94‑1 (MQ=60)
                                                                CTGCAAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGC                                >  SRR4302533.503158/1‑94 (MQ=60)
                                                                   CAAACGCGGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGT                             <  SRR4302533.452371/94‑1 (MQ=60)
                                                                    AAACGCTGCTGCATCCCGCCGG‑AAA‑ATGGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCTCCTCTTCCAGCCACTTCTGCGCGGTG                            <  SRR4302533.323091/94‑1 (MQ=60)
                                                                    AAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCCCCTCTTCCAGCCACTTCTGCGCGGTG                            <  SRR4302533.3221653/94‑1 (MQ=60)
                                                                    AAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTG                            >  SRR4302533.4259469/1‑94 (MQ=60)
                                                                    AAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTG                            >  SRR4302533.4517097/1‑94 (MQ=60)
                                                                    AAACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTATGCGCGGTG                            >  SRR4302533.2204340/1‑94 (MQ=60)
                                                                     AACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGG                           >  SRR4302533.6281157/1‑94 (MQ=60)
                                                                      ACGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGC                          <  SRR4302533.4115674/94‑1 (MQ=60)
                                                                       CGCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCA                         >  SRR4302533.1586022/1‑94 (MQ=60)
                                                                        GCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCAC                        >  SRR4302533.3332338/1‑94 (MQ=60)
                                                                        GCTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCAC                        >  SRR4302533.2644954/1‑94 (MQ=60)
                                                                         CTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACG                       >  SRR4302533.5165340/1‑94 (MQ=60)
                                                                         CTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACG                       <  SRR4302533.2893874/94‑1 (MQ=60)
                                                                         CTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACG                       >  SRR4302533.3272764/1‑94 (MQ=60)
                                                                         CTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACG                       <  SRR4302533.3982738/94‑1 (MQ=60)
                                                                         CTGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCAACCTCTTCCAGCCACTTCTGCGCGGTGGCACG                       >  SRR4302533.1940350/1‑94 (MQ=60)
                                                                          TGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGA                      <  SRR4302533.6230700/94‑1 (MQ=60)
                                                                          TGCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGA                      >  SRR4302533.1320670/1‑94 (MQ=60)
                                                                           GCTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAA                     >  SRR4302533.1157948/1‑94 (MQ=60)
                                                                            CTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAAT                    <  SRR4302533.5485733/94‑1 (MQ=60)
                                                                            CTGCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGCGGCACGAAT                    >  SRR4302533.4953404/1‑94 (MQ=60)
                                                                              GCATGCCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCTATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATAT                  <  SRR4302533.5566325/94‑1 (MQ=60)
                                                                              GCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATAT                  <  SRR4302533.594151/94‑1 (MQ=60)
                                                                              GCATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATAT                  <  SRR4302533.396224/94‑1 (MQ=60)
                                                                               CATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATC                 >  SRR4302533.4674045/1‑94 (MQ=60)
                                                                               CATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATC                 >  SRR4302533.3012345/1‑94 (MQ=60)
                                                                               CATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATC                 <  SRR4302533.3366853/94‑1 (MQ=60)
                                                                               CATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATC                 >  SRR4302533.6264203/1‑94 (MQ=60)
                                                                               CATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATC                 >  SRR4302533.3675743/1‑94 (MQ=60)
                                                                               CATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATC                 <  SRR4302533.4487913/94‑1 (MQ=60)
                                                                                ATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCCCCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCG                <  SRR4302533.1422381/94‑1 (MQ=60)
                                                                                ATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCG                <  SRR4302533.874484/94‑1 (MQ=60)
                                                                                ATACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCG                <  SRR4302533.764416/94‑1 (MQ=60)
                                                                                 TACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGC               >  SRR4302533.1642/1‑94 (MQ=60)
                                                                                  CCCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCCCCCCCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCC              <  SRR4302533.5510656/94‑1 (MQ=60)
                                                                                  ACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCC              >  SRR4302533.5479869/1‑94 (MQ=60)
                                                                                  ACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCC              <  SRR4302533.1541639/94‑1 (MQ=60)
                                                                                  ACCGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCC              >  SRR4302533.4754962/1‑94 (MQ=60)
                                                                                    CGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCTCCTCTTCCAGCCACTTCTGCGtgaaggcactaatagctcagt            >  SRR4302533.3383267/1‑73 (MQ=60)
                                                                                    CGCCGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGT            <  SRR4302533.2531746/94‑1 (MQ=60)
                                                                                      ccgaaAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAA          <  SRR4302533.642626/89‑1 (MQ=60)
                                                                                       CGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAAT         <  SRR4302533.2566096/94‑1 (MQ=60)
                                                                                       CGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAAT         >  SRR4302533.6323687/1‑94 (MQ=60)
                                                                                       CGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAAT         >  SRR4302533.1201727/1‑94 (MQ=60)
                                                                                       CGGAAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAAT         >  SRR4302533.582531/1‑94 (MQ=60)
                                                                                        ggaAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATG        >  SRR4302533.4235682/4‑94 (MQ=60)
                                                                                        ggaAAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATG        >  SRR4302533.5171259/4‑94 (MQ=60)
                                                                                        GA‑AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGATGGCGGAAATCTACACATCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGA       <  SRR4302533.6341724/94‑1 (MQ=60)
                                                                                         A‑AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGAC      <  SRR4302533.1183738/94‑1 (MQ=60)
                                                                                         A‑AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGAC      <  SRR4302533.1713958/94‑1 (MQ=60)
                                                                                         A‑AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGAC      >  SRR4302533.3720633/1‑94 (MQ=60)
                                                                                           AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGACG     <  SRR4302533.3500431/94‑1 (MQ=60)
                                                                                           AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGACG     >  SRR4302533.4217340/1‑94 (MQ=60)
                                                                                               A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGGATATCGCCCTAATGACGTGC  >  SRR4302533.1188578/1‑94 (MQ=60)
                                                                                               A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGACGTGC  <  SRR4302533.2611649/94‑1 (MQ=60)
                                                                                               A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGACGTGC  >  SRR4302533.2113731/1‑94 (MQ=60)
                                                                                               A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGACGTGC  <  SRR4302533.2107619/94‑1 (MQ=60)
                                                                                               |                                                                                              
CCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGG‑AAA‑A‑GGTGGTCGGCAGGTCGTCGATCCGGTTGGCGGGAATCTCCACCTCTTCCAGCCACTTCTGCGCGGTGGCACGAATATCGCCGTAATGACGTGC  >  NC_000913/4315814‑4316000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑