Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,356,775 C→T A279T (GCC→ACC)  cadB ← transport of lysine/cadaverine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,356,7750CT100.0% 828.7 / NA 238A279T (GCC→ACC) cadBtransport of lysine/cadaverine
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (120/118);  total (120/118)

Only 100 of 247 total aligned reads displayed.

CTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACA  >  NC_000913/4356685‑4356863
                                                                                          |                                                                                        
  ttCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGgtggtg                                                                                      <  1:2524567/93‑1 (MQ=255)
   tCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAggtggtca                                                                                     <  2:3295543/93‑3 (MQ=255)
   tCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGa                                                                                     <  1:3250605/93‑1 (MQ=255)
    cGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGaa                                                                                    <  1:6138309/93‑1 (MQ=255)
    cGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGaa                                                                                    <  1:3562889/93‑1 (MQ=255)
     gggAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAAt                                                                                   >  2:5610451/1‑93 (MQ=255)
      ggAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATg                                                                                  >  2:5472400/1‑93 (MQ=255)
       gAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGc                                                                                 >  1:5694667/1‑93 (MQ=255)
         aGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAg                                                                               >  1:2030685/1‑93 (MQ=255)
           ttACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGaa                                                                             >  2:2544801/1‑93 (MQ=255)
             aCCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAc                                                                           <  2:4295724/93‑1 (MQ=255)
             aCCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAc                                                                           <  2:1757176/93‑1 (MQ=255)
             aCCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAc                                                                           >  2:1484982/1‑93 (MQ=255)
              ccGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAcc                                                                          >  2:3612920/1‑93 (MQ=255)
              ccGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAcc                                                                          >  2:3920433/1‑93 (MQ=255)
              ccGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAcc                                                                          >  2:4762696/1‑93 (MQ=255)
              ccGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAcc                                                                          >  1:648128/1‑93 (MQ=255)
              ccGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAAcc                                                                          <  2:6007822/93‑1 (MQ=255)
               cgtcgtTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCa                                                                         <  2:873743/93‑1 (MQ=255)
               cgtcgtTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCa                                                                         <  1:4705646/93‑1 (MQ=255)
                gtcgtTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAg                                                                        <  2:6091399/93‑1 (MQ=255)
                  cgtTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAgcg                                                                      <  2:4399353/93‑1 (MQ=255)
                   gtTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAgcgg                                                                     >  1:3304566/1‑93 (MQ=255)
                    ttAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAgcggc                                                                    <  1:1748410/93‑1 (MQ=255)
                      aGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGgcgc                                                                  <  2:1721139/93‑1 (MQ=255)
                      aGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGgcgc                                                                  >  1:4053537/1‑93 (MQ=255)
                       gCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCa                                                                 >  2:3739748/1‑93 (MQ=255)
                        cGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAg                                                                >  1:5312065/1‑93 (MQ=255)
                         ggCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGc                                                               <  2:5065253/93‑1 (MQ=255)
                         ggCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGc                                                               <  1:2301039/93‑1 (MQ=255)
                           cACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGccc                                                             >  1:122768/1‑93 (MQ=255)
                             cGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAg                                                           >  1:4549037/1‑93 (MQ=255)
                             cGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAg                                                           >  1:693332/1‑93 (MQ=255)
                                acacCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTa                                                        <  2:2050606/93‑1 (MQ=255)
                                 cacCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTAc                                                       <  2:5900042/93‑1 (MQ=255)
                                 cacCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTAc                                                       <  1:3996813/93‑1 (MQ=255)
                                  acCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTAcc                                                      <  1:113850/93‑1 (MQ=255)
                                  acCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTAcc                                                      >  2:3796868/1‑93 (MQ=255)
                                   cctgcctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCg                                                     >  2:2117437/1‑93 (MQ=255)
                                   cctgcctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCg                                                     >  1:1677087/1‑93 (MQ=255)
                                    ctgcctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGa                                                    <  1:3218794/93‑1 (MQ=255)
                                      gcctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAgg                                                  >  2:4874196/1‑93 (MQ=255)
                                       cctgGCTTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGa                                                 <  1:4164383/93‑1 (MQ=255)
                                       cctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGa                                                 <  2:4228037/93‑1 (MQ=255)
                                        ctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGAt                                                >  1:1246391/1‑93 (MQ=255)
                                        ctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGAt                                                >  1:2568235/1‑93 (MQ=255)
                                         tgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATa                                               <  2:189314/93‑1 (MQ=255)
                                         tgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATa                                               >  2:4932214/1‑93 (MQ=255)
                                         tgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATa                                               >  2:1259372/1‑93 (MQ=255)
                                         tgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATa                                               <  2:1569326/93‑1 (MQ=255)
                                          ggCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAg                                              <  2:2947867/93‑1 (MQ=255)
                                             cTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTg                                           >  2:2850805/1‑93 (MQ=255)
                                              tACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGa                                          <  2:3849888/93‑1 (MQ=255)
                                              tACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGa                                          <  1:5435026/93‑1 (MQ=255)
                                               aCCAACATCATGCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGaa                                         <  2:1160872/93‑1 (MQ=255)
                                               aCCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGaa                                         <  2:107721/93‑1 (MQ=255)
                                                ccAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAg                                        <  1:5220828/93‑1 (MQ=255)
                                                 cAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGc                                       >  2:4583278/1‑93 (MQ=255)
                                                 cAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGc                                       <  2:5404024/93‑1 (MQ=255)
                                                   aCATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCAc                                     >  2:5327921/1‑93 (MQ=255)
                                                    catcatCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACt                                    >  2:3422291/1‑93 (MQ=255)
                                                      tcatcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGa                                  <  2:2613626/93‑1 (MQ=255)
                                                       catcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGAt                                 <  1:5940403/93‑1 (MQ=255)
                                                       catcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGAt                                 <  1:1060434/93‑1 (MQ=255)
                                                       catcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGAt                                 >  2:4985683/1‑93 (MQ=255)
                                                        atcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGAtt                                <  2:3122971/93‑1 (MQ=255)
                                                        atcCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGAtt                                <  2:2395061/93‑1 (MQ=255)
                                                           cAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCa                             >  2:3223705/1‑93 (MQ=255)
                                                             ggAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCaaa                           <  2:5701781/93‑1 (MQ=255)
                                                                gCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGACACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACgg                        <  1:3703025/93‑1 (MQ=255)
                                                                gCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACgg                        >  1:1081016/1‑93 (MQ=255)
                                                                gCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACgg                        >  2:4058546/1‑93 (MQ=255)
                                                                gCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACgg                        <  1:2088705/93‑1 (MQ=255)
                                                                  ccAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAg                      <  1:1625208/93‑1 (MQ=255)
                                                                  ccAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAg                      >  2:4680507/1‑93 (MQ=255)
                                                                   cAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGc                     <  1:49513/93‑1 (MQ=255)
                                                                    agagAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCa                    >  2:2753206/1‑93 (MQ=255)
                                                                     gagaAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCAc                   <  2:4085310/93‑1 (MQ=255)
                                                                     gagaAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCAc                   <  2:418213/93‑1 (MQ=255)
                                                                     gagaAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCAc                   >  2:6182894/1‑93 (MQ=255)
                                                                     gagaAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCAc                   >  1:4357629/1‑93 (MQ=255)
                                                                     gagaAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCAc                   <  2:6058647/93‑1 (MQ=255)
                                                                        aaGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCgg                <  1:5620339/93‑1 (MQ=255)
                                                                         aGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGa               >  1:1790865/1‑93 (MQ=255)
                                                                         aGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGa               <  1:981695/93‑1 (MQ=255)
                                                                           tCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAg             <  2:5795546/93‑1 (MQ=255)
                                                                            cAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAgc            >  2:5034621/1‑93 (MQ=255)
                                                                             aGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAgcc           <  1:3000911/93‑1 (MQ=255)
                                                                              ggCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAgccg          >  1:5512840/1‑93 (MQ=255)
                                                                                 aCGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCa       <  2:893562/93‑1 (MQ=255)
                                                                                  cGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCAt      <  1:5058856/93‑1 (MQ=255)
                                                                                  cGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCAt      >  2:2200665/1‑93 (MQ=255)
                                                                                   gCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCAtt     >  1:620761/1‑93 (MQ=255)
                                                                                   gCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCAtt     >  2:6292698/1‑93 (MQ=255)
                                                                                    cAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCGCCGGAAGCCGCCATTa    <  1:3177932/93‑1 (MQ=255)
                                                                                    cAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTa    >  2:3466994/1‑93 (MQ=255)
                                                                                     aaaGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTAc   >  1:2156771/1‑93 (MQ=255)
                                                                                     aaaGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTAc   <  1:5082666/93‑1 (MQ=255)
                                                                                     aaaGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACAGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTAc   <  1:3415408/93‑1 (MQ=255)
                                                                                      aaGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACa  <  2:4453938/93‑1 (MQ=255)
                                                                                          |                                                                                        
CTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACA  >  NC_000913/4356685‑4356863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGA  >  NC_000913/4356683‑4356868
                                                                                            |                                                                                             
ATCTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTG                                                                                              <  SRR4302533.422642/94‑1 (MQ=60)
 ACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGG                                                                                             <  SRR4302533.1213352/94‑1 (MQ=60)
 ACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGG                                                                                             >  SRR4302533.5741892/1‑94 (MQ=60)
  CTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGT                                                                                            >  SRR4302533.5391843/1‑94 (MQ=60)
  CTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGT                                                                                            >  SRR4302533.2237539/1‑94 (MQ=60)
    TTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGA                                                                                          <  SRR4302533.2540115/94‑1 (MQ=60)
     TCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAA                                                                                         <  SRR4302533.3270648/94‑1 (MQ=60)
      CGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAAT                                                                                        <  SRR4302533.878017/94‑1 (MQ=60)
      CGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAAT                                                                                        <  SRR4302533.3584841/94‑1 (MQ=60)
      CGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAAT                                                                                        <  SRR4302533.6175286/94‑1 (MQ=60)
        GGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGC                                                                                      >  SRR4302533.5729057/1‑94 (MQ=60)
          AAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAG                                                                                    >  SRR4302533.2043218/1‑94 (MQ=60)
           AGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGA                                                                                   <  SRR4302533.5213606/94‑1 (MQ=60)
            GTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAA                                                                                  <  SRR4302533.122899/94‑1 (MQ=60)
               TCCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACC                                                                               <  SRR4302533.1853135/94‑1 (MQ=60)
               ACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACC                                                                               >  SRR4302533.651949/1‑94 (MQ=60)
                CCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCA                                                                              >  SRR4302533.2153267/1‑94 (MQ=60)
                CCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCA                                                                              <  SRR4302533.4117299/94‑1 (MQ=60)
                 CGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAG                                                                             <  SRR4302533.403370/94‑1 (MQ=60)
                 CGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAG                                                                             <  SRR4302533.4734164/94‑1 (MQ=60)
                  GTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGC                                                                            <  SRR4302533.1242528/94‑1 (MQ=60)
                  GTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGC                                                                            >  SRR4302533.1109965/1‑94 (MQ=60)
                    CGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGG                                                                          <  SRR4302533.4583284/94‑1 (MQ=60)
                    CGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGG                                                                          >  SRR4302533.3324974/1‑94 (MQ=60)
                      TTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGTGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCG                                                                        <  SRR4302533.4470652/94‑1 (MQ=60)
                      TTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCG                                                                        <  SRR4302533.1759054/94‑1 (MQ=60)
                      TTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCG                                                                        <  SRR4302533.4981628/94‑1 (MQ=60)
                       TAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGC                                                                       >  SRR4302533.4078221/1‑94 (MQ=60)
                         GCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAG                                                                     >  SRR4302533.5343900/1‑94 (MQ=60)
                           GGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCC                                                                   <  SRR4302533.2315300/94‑1 (MQ=60)
                           GGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCC                                                                   >  SRR4302533.513970/1‑94 (MQ=60)
                            GCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCC                                                                  >  SRR4302533.2321525/1‑94 (MQ=60)
                            GCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCC                                                                  >  SRR4302533.123541/1‑94 (MQ=60)
                             CACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCA                                                                 <  SRR4302533.797751/94‑1 (MQ=60)
                             CACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCA                                                                 >  SRR4302533.5481851/1‑94 (MQ=60)
                              ACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAG                                                                >  SRR4302533.4576622/1‑94 (MQ=60)
                              ACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAG                                                                >  SRR4302533.697428/1‑94 (MQ=60)
                               CGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGT                                                               <  SRR4302533.42895/94‑1 (MQ=60)
                                GTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTT                                                              <  SRR4302533.3246042/94‑1 (MQ=60)
                                 TACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTA                                                             >  SRR4302533.191616/1‑94 (MQ=60)
                                   CACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACC                                                           <  SRR4302533.4021151/94‑1 (MQ=60)
                                    ACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCG                                                          >  SRR4302533.1687241/1‑94 (MQ=60)
                                    ACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCG                                                          <  SRR4302533.114573/94‑1 (MQ=60)
                                      CTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAG                                                        >  SRR4302533.5676721/1‑94 (MQ=60)
                                      CTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAG                                                        <  SRR4302533.3238641/94‑1 (MQ=60)
                                      CTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAG                                                        >  SRR4302533.1221165/1‑94 (MQ=60)
                                       TGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGG                                                       <  SRR4302533.1663523/94‑1 (MQ=60)
                                         CCTGGCTTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGAT                                                     <  SRR4302533.4189612/94‑1 (MQ=60)
                                         CCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGAT                                                     <  SRR4302533.650094/94‑1 (MQ=60)
                                         CCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGAT                                                     <  SRR4302533.3070414/94‑1 (MQ=60)
                                         CCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGAT                                                     >  SRR4302533.1253831/1‑94 (MQ=60)
                                         CCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGAT                                                     >  SRR4302533.2584039/1‑94 (MQ=60)
                                          CTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATA                                                    >  SRR4302533.2342126/1‑94 (MQ=60)
                                           TGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAG                                                   <  SRR4302533.4714138/94‑1 (MQ=60)
                                           TGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAG                                                   >  SRR4302533.5600211/1‑94 (MQ=60)
                                            GGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGT                                                  <  SRR4302533.3433674/94‑1 (MQ=60)
                                             GCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTT                                                 >  SRR4302533.2679024/1‑94 (MQ=60)
                                             GCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTT                                                 <  SRR4302533.2578124/94‑1 (MQ=60)
                                              CCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTG                                                >  SRR4302533.4987533/1‑94 (MQ=60)
                                               CTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGA                                               >  SRR4302533.5676875/1‑94 (MQ=60)
                                                TACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAA                                              <  SRR4302533.5467757/94‑1 (MQ=60)
                                                 ACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAG                                             >  SRR4302533.1362018/1‑94 (MQ=60)
                                                 ACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAG                                             <  SRR4302533.3277039/94‑1 (MQ=60)
                                                  CCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGC                                            <  SRR4302533.5252247/94‑1 (MQ=60)
                                                      CATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTG                                        >  SRR4302533.62089/1‑94 (MQ=60)
                                                        TCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGAT                                      <  SRR4302533.3458124/94‑1 (MQ=60)
                                                         CATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATT                                     <  SRR4302533.1066668/94‑1 (MQ=60)
                                                         CATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATT                                     <  SRR4302533.5976325/94‑1 (MQ=60)
                                                             CAGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAA                                 >  SRR4302533.3856520/1‑94 (MQ=60)
                                                              AGGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAA                                <  SRR4302533.5540119/94‑1 (MQ=60)
                                                               GGAGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAAC                               <  SRR4302533.402359/94‑1 (MQ=60)
                                                                 AGCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGG                             >  SRR4302533.1087393/1‑94 (MQ=60)
                                                                  GCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGACACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGA                            <  SRR4302533.3725767/94‑1 (MQ=60)
                                                                  GCCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGA                            <  SRR4302533.2101565/94‑1 (MQ=60)
                                                                   CCCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAG                           <  SRR4302533.2106029/94‑1 (MQ=60)
                                                                    CCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGC                          <  SRR4302533.1635052/94‑1 (MQ=60)
                                                                    CCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGC                          >  SRR4302533.6063601/1‑94 (MQ=60)
                                                                    CCAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGC                          <  SRR4302533.2715281/94‑1 (MQ=60)
                                                                     CAGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCA                         <  SRR4302533.49855/94‑1 (MQ=60)
                                                                      AGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCAC                        >  SRR4302533.4383931/1‑94 (MQ=60)
                                                                      AGAGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCAC                        >  SRR4302533.3712911/1‑94 (MQ=60)
                                                                        AGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCG                      >  SRR4302533.6332723/1‑94 (MQ=60)
                                                                        AGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCG                      <  SRR4302533.1732056/94‑1 (MQ=60)
                                                                        AGAAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCG                      >  SRR4302533.3385045/1‑94 (MQ=60)
                                                                          AAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGA                    <  SRR4302533.5654309/94‑1 (MQ=60)
                                                                          AAGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGA                    >  SRR4302533.1801755/1‑94 (MQ=60)
                                                                          AAGTCAGGCACGCAAAGGTGGGGAATGCAGAAACCAGCGGCGCAGACCAGTCACGGAGGGACGTTGAGGCACTGATAGAAAACGGAGCACCGGA                    >  SRR4302533.1801754/1‑94 (MQ=32)
                                                                           AGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAA                   <  SRR4302533.987449/94‑1 (MQ=60)
                                                                           AGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAA                   >  SRR4302533.3212669/1‑94 (MQ=60)
                                                                           AGTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGAACCGGAA                   >  SRR4302533.5102391/1‑94 (MQ=60)
                                                                            GTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAG                  <  SRR4302533.1079571/94‑1 (MQ=60)
                                                                            GTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAG                  >  SRR4302533.136411/1‑94 (MQ=60)
                                                                            GTCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAG                  >  SRR4302533.1738914/1‑94 (MQ=60)
                                                                             TCAGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGC                 >  SRR4302533.4334517/1‑94 (MQ=60)
                                                                               AGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAATGGAGCACCGGAAGCCC               >  SRR4302533.2093723/1‑94 (MQ=60)
                                                                               AGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCG               >  SRR4302533.5546119/1‑94 (MQ=60)
                                                                               AGGCACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCG               <  SRR4302533.3019263/94‑1 (MQ=60)
                                                                                  CACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCA            >  SRR4302533.670579/1‑94 (MQ=60)
                                                                                  CACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCA            >  SRR4302533.4957885/1‑94 (MQ=60)
                                                                                   ACGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCAT           >  SRR4302533.5290902/1‑94 (MQ=60)
                                                                                    CGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATT          <  SRR4302533.3242054/94‑1 (MQ=60)
                                                                                    CGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATT          >  SRR4302533.624408/1‑94 (MQ=60)
                                                                                    CGCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATT          <  SRR4302533.5089425/94‑1 (MQ=60)
                                                                                     GCAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTA         <  SRR4302533.6129250/94‑1 (MQ=60)
                                                                                      CAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCGCCGGAAGCCGCCATTAC        <  SRR4302533.3197480/94‑1 (MQ=60)
                                                                                      CAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTAC        <  SRR4302533.3590462/94‑1 (MQ=60)
                                                                                      CAAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTAC        >  SRR4302533.2170049/1‑94 (MQ=60)
                                                                                       AAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACA       <  SRR4302533.5113386/94‑1 (MQ=60)
                                                                                       AAAGGTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACAGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACA       <  SRR4302533.3436512/94‑1 (MQ=60)
                                                                                           GTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAG   <  SRR4302533.3320431/94‑1 (MQ=60)
                                                                                           GTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAG   <  SRR4302533.5924364/94‑1 (MQ=60)
                                                                                           GTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAG   >  SRR4302533.4066898/1‑94 (MQ=60)
                                                                                           GTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAG   >  SRR4302533.1760483/1‑94 (MQ=60)
                                                                                           GTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAG   <  SRR4302533.1564350/94‑1 (MQ=60)
                                                                                           GTGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAG   <  SRR4302533.5386146/94‑1 (MQ=60)
                                                                                            TGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGA  <  SRR4302533.554645/94‑1 (MQ=60)
                                                                                            |                                                                                             
AACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGA  >  NC_000913/4356683‑4356868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑