Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,385,717 T→C N411S (AAC→AGC)  yjeP ← putative periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,385,7170TC100.0% 825.0 / NA 254N411S (AAC→AGC) yjePputative periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (141/113);  total (141/113)

Only 100 of 260 total aligned reads displayed.

GCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGT  >  NC_000913/4385627‑4385806
                                                                                          |                                                                                         
gCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTg                                                                                         <  2:4056169/93‑1 (MQ=255)
  gATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGga                                                                                       <  1:5384706/93‑1 (MQ=255)
  gATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGga                                                                                       <  1:3479613/93‑1 (MQ=255)
    tGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGgaga                                                                                     >  1:2759597/1‑93 (MQ=255)
     ggTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGAc                                                                                    <  1:5130250/93‑1 (MQ=255)
     ggTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGAc                                                                                    <  1:5986067/93‑1 (MQ=255)
     ggTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGAc                                                                                    >  2:282754/1‑93 (MQ=255)
     ggTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGAc                                                                                    <  1:4831443/93‑1 (MQ=255)
     ggTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGAc                                                                                    >  2:1198885/1‑93 (MQ=255)
       tCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACtt                                                                                  <  1:1694935/93‑1 (MQ=255)
         aTCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTc                                                                                >  2:3770786/1‑93 (MQ=255)
         aTCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTc                                                                                >  1:2220436/1‑93 (MQ=255)
         aTCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTc                                                                                <  1:3125553/93‑1 (MQ=255)
            gggCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGc                                                                             <  1:1046257/93‑1 (MQ=255)
             ggCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCt                                                                            >  2:126304/1‑93 (MQ=255)
               cgcACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTg                                                                          <  1:2020345/93‑1 (MQ=255)
                gcACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTgg                                                                         >  2:4715260/1‑93 (MQ=255)
                 cACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGt                                                                        <  2:395144/93‑1 (MQ=255)
                 cACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGt                                                                        <  1:4101224/93‑1 (MQ=255)
                  aCGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTc                                                                       >  1:2926281/1‑93 (MQ=255)
                   cGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCa                                                                      >  1:6185332/1‑93 (MQ=255)
                     tCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGt                                                                    >  1:1779625/1‑93 (MQ=255)
                      cAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGtt                                                                   >  1:2609265/1‑93 (MQ=255)
                       agagGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTc                                                                  >  1:1547516/1‑93 (MQ=255)
                        gagTTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCTCCTCTTTCAGCGCATCCTCCAGTAGCCCGCTGGAGACTTTCAGCTTGGTCAGTTcc                                                                 <  2:6011995/93‑1 (MQ=255)
                        gagGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTcc                                                                 >  2:5671087/1‑93 (MQ=255)
                         agGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCa                                                                >  1:3750632/1‑93 (MQ=255)
                            tCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATGCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAAta                                                             >  1:1278259/1‑93 (MQ=255)
                            tCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAAta                                                             >  2:4731025/1‑93 (MQ=255)
                              cAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAAtagt                                                           >  1:3556/1‑93 (MQ=255)
                              cAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAAtagt                                                           >  1:2785190/1‑93 (MQ=255)
                               aGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAAtagta                                                          >  2:5897128/1‑93 (MQ=255)
                                gAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCGTCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAAtagtag                                                         >  1:4427652/1‑93 (MQ=255)
                                gAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAAtagtag                                                         >  2:2488087/1‑93 (MQ=255)
                                  aCAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCg                                                       <  2:1943054/93‑1 (MQ=255)
                                  aCAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCg                                                       >  2:3682244/1‑93 (MQ=255)
                                   cAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCgt                                                      <  2:5864582/93‑1 (MQ=255)
                                      aTAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCATTTGGCCGCTTGAGACTTTCAGCTTGGTAAGGTCCAATAGTAGCGTGTc                                                   >  2:2646135/1‑93 (MQ=255)
                                       tAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTcc                                                  >  1:3965892/1‑92 (MQ=255)
                                         gCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGcc                                                >  2:6151670/1‑93 (MQ=255)
                                         gCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGATTGGTCAGTTCCAATAGTAGCGTGTCGcc                                                >  2:1335201/1‑93 (MQ=255)
                                           ggTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAc                                              <  1:6290991/93‑1 (MQ=255)
                                            gTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAcc                                             >  2:2942243/1‑93 (MQ=255)
                                            gTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAcc                                             >  1:3872852/1‑93 (MQ=255)
                                            gTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAcc                                             >  1:2379830/1‑93 (MQ=255)
                                             tGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAccc                                            <  2:3704569/93‑1 (MQ=255)
                                              gCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCt                                           <  1:5064840/93‑1 (MQ=255)
                                              gCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCt                                           >  1:3284161/1‑93 (MQ=255)
                                               cGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTg                                          >  2:4273184/1‑93 (MQ=255)
                                                 ttGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGca                                        >  1:4919496/1‑93 (MQ=255)
                                                  tGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGcaa                                       <  1:5724088/93‑1 (MQ=255)
                                                  tGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGcaa                                       >  1:653116/1‑93 (MQ=255)
                                                     ttCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGcaacaa                                    <  1:1221133/93‑1 (MQ=255)
                                                       cGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATg                                  <  2:4683466/93‑1 (MQ=255)
                                                        gTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGa                                 >  2:175943/1‑93 (MQ=255)
                                                         ttCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAg                                <  1:106556/93‑1 (MQ=255)
                                                         ttCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAg                                >  2:5064610/1‑93 (MQ=255)
                                                         ttCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAg                                <  2:6120504/93‑1 (MQ=255)
                                                          tCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGt                               <  2:5192095/93‑1 (MQ=255)
                                                          tCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGt                               <  2:3422013/93‑1 (MQ=255)
                                                           cACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGtt                              >  1:3694303/1‑93 (MQ=255)
                                                           cACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGtt                              >  1:5219944/1‑93 (MQ=255)
                                                            cccTCTTTCAGCGCACCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTc                             <  2:530557/92‑1 (MQ=255)
                                                             ccTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCa                            <  2:2341261/93‑1 (MQ=255)
                                                              ctctTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCACCAATGAGTTCAg                           >  1:3599580/1‑93 (MQ=255)
                                                               tctGTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGc                          <  1:173406/93‑1 (MQ=255)
                                                                 tttCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCaa                        <  2:1816401/93‑1 (MQ=255)
                                                                 tttCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCaa                        >  1:4594162/1‑93 (MQ=255)
                                                                     aGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCa                    <  2:1672329/93‑1 (MQ=255)
                                                                        gcATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGc                 <  1:2976881/93‑1 (MQ=255)
                                                                        gcATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGc                 <  2:5503245/93‑1 (MQ=255)
                                                                        gcATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGc                 >  1:1385739/1‑93 (MQ=255)
                                                                         cATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGGTCAGCAACTCACGCt                >  1:3850960/1‑93 (MQ=255)
                                                                          aTCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTg               >  1:2930250/1‑93 (MQ=255)
                                                                           tcctccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGa              >  2:6078414/1‑93 (MQ=255)
                                                                           tcctccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGa              >  2:4942434/1‑93 (MQ=255)
                                                                           tcctccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGCTCATCAACTCACGCTGa              >  2:6302854/1‑93 (MQ=255)
                                                                            cctccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAg             >  1:2677123/1‑93 (MQ=255)
                                                                            cctccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAg             <  1:610037/93‑1 (MQ=255)
                                                                             ctccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGt            <  2:5781833/93‑1 (MQ=255)
                                                                             ctccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGt            <  2:2440384/93‑1 (MQ=255)
                                                                              tccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTg           <  1:3326878/93‑1 (MQ=255)
                                                                              tccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTg           <  2:778902/93‑1 (MQ=255)
                                                                              tccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTg           >  2:1630844/1‑93 (MQ=255)
                                                                              tccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTg           >  1:4365450/1‑93 (MQ=255)
                                                                               ccAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgc          <  1:2494214/93‑1 (MQ=255)
                                                                                cAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgcg         >  2:2902266/1‑93 (MQ=255)
                                                                                 aGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgcgc        >  2:2814191/1‑93 (MQ=255)
                                                                                 aGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgcgc        >  1:4923216/1‑93 (MQ=255)
                                                                                  gTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCa       >  2:3348458/1‑93 (MQ=255)
                                                                                  gTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCa       <  2:1562846/93‑1 (MQ=255)
                                                                                  gTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCa       <  2:444586/93‑1 (MQ=255)
                                                                                  gTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCa       >  1:5511587/1‑93 (MQ=255)
                                                                                  gTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCa       <  2:5349546/93‑1 (MQ=255)
                                                                                   gtGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAg      <  2:3116610/92‑1 (MQ=255)
                                                                                    tGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGt     <  1:3914783/93‑1 (MQ=255)
                                                                                     gCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGtt    <  1:5897255/93‑1 (MQ=255)
                                                                                     gCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGtt    >  2:3942077/1‑93 (MQ=255)
                                                                                       ccGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTtgt  <  1:885264/93‑1 (MQ=255)
                                                                                       ccGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTtgt  <  2:4150519/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGT  >  NC_000913/4385627‑4385806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTT  >  NC_000913/4385624‑4385810
                                                                                             |                                                                                             
CAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGC                                                                                               >  SRR4302533.5214995/1‑94 (MQ=60)
 AGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCACTTTCAGCGCATCCTCCAGTTGCCCGCT                                                                                              >  SRR4302533.519323/1‑94 (MQ=60)
   GCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGG                                                                                            <  SRR4302533.3951211/94‑1 (MQ=60)
    CGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGA                                                                                           >  SRR4302533.12414/1‑94 (MQ=60)
     GATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAG                                                                                          >  SRR4302533.1713574/1‑94 (MQ=60)
     GATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAG                                                                                          <  SRR4302533.5417058/94‑1 (MQ=60)
     GATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAG                                                                                          <  SRR4302533.3501082/94‑1 (MQ=60)
      ATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGA                                                                                         >  SRR4302533.2555794/1‑94 (MQ=60)
      ATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGA                                                                                         >  SRR4302533.2776511/1‑94 (MQ=60)
        GGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACT                                                                                       <  SRR4302533.6022318/94‑1 (MQ=60)
        GGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACT                                                                                       <  SRR4302533.5161241/94‑1 (MQ=60)
        GGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACT                                                                                       <  SRR4302533.4860725/94‑1 (MQ=60)
          TCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTT                                                                                     <  SRR4302533.177364/94‑1 (MQ=60)
          TCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTT                                                                                     <  SRR4302533.1705202/94‑1 (MQ=60)
           CATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTC                                                                                    >  SRR4302533.2648559/1‑94 (MQ=60)
           CATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTC                                                                                    >  SRR4302533.2234144/1‑94 (MQ=60)
            ATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCA                                                                                   <  SRR4302533.3144761/94‑1 (MQ=60)
            ATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCA                                                                                   >  SRR4302533.653844/1‑94 (MQ=60)
               GGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCT                                                                                <  SRR4302533.1052405/94‑1 (MQ=60)
               GGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCT                                                                                <  SRR4302533.4576689/94‑1 (MQ=60)
                  CGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGG                                                                             >  SRR4302533.489937/1‑94 (MQ=60)
                  CGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGG                                                                             <  SRR4302533.2032787/94‑1 (MQ=60)
                  CGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGG                                                                             >  SRR4302533.1473722/1‑94 (MQ=60)
                   GCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTACCCGCTGGAGACTTTCAGCTTGGT                                                                            <  SRR4302533.2979354/94‑1 (MQ=60)
                    CACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTC                                                                           <  SRR4302533.4126181/94‑1 (MQ=60)
                    CACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTC                                                                           <  SRR4302533.2323500/94‑1 (MQ=60)
                    CACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTC                                                                           >  SRR4302533.2944185/1‑94 (MQ=60)
                     ACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCA                                                                          <  SRR4302533.1937636/94‑1 (MQ=60)
                     ACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCA                                                                          >  SRR4302533.6222465/1‑94 (MQ=60)
                      CGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTTGAGACTTTCAGCTTGGTCAG                                                                         >  SRR4302533.3021321/1‑94 (MQ=60)
                      CGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAG                                                                         <  SRR4302533.4922805/94‑1 (MQ=60)
                       GTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGT                                                                        >  SRR4302533.1790460/1‑94 (MQ=60)
                       GTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGT                                                                        <  SRR4302533.5101009/94‑1 (MQ=60)
                       GTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGT                                                                        <  SRR4302533.3303020/94‑1 (MQ=60)
                        TCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTT                                                                       >  SRR4302533.2946124/1‑94 (MQ=60)
                        TCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTT                                                                       >  SRR4302533.2706419/1‑94 (MQ=60)
                        TCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTT                                                                       >  SRR4302533.2625285/1‑94 (MQ=60)
                         CAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTC                                                                      >  SRR4302533.1556841/1‑94 (MQ=60)
                          AGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCC                                                                     >  SRR4302533.6122497/1‑94 (MQ=60)
                           GAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCA                                                                    >  SRR4302533.3773628/1‑94 (MQ=60)
                            AGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAA                                                                   >  SRR4302533.6256199/1‑94 (MQ=60)
                              GTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATGCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATA                                                                 >  SRR4302533.1285942/1‑94 (MQ=60)
                              GTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATA                                                                 >  SRR4302533.3740957/1‑94 (MQ=60)
                               TCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAG                                                                >  SRR4302533.355098/1‑94 (MQ=60)
                                CCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGT                                                               >  SRR4302533.3587/1‑94 (MQ=60)
                                CCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGT                                                               >  SRR4302533.2802305/1‑94 (MQ=60)
                                CCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGT                                                               >  SRR4302533.3641431/1‑94 (MQ=60)
                                 CAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTA                                                              <  SRR4302533.5461708/94‑1 (MQ=60)
                                  AGAACAGATAGCGTTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAG                                                             <  SRR4302533.21292/94‑1 (MQ=60)
                                  AGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCGTCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAG                                                             >  SRR4302533.4454415/1‑94 (MQ=60)
                                   GAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGC                                                            >  SRR4302533.2686466/1‑94 (MQ=60)
                                   GAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGC                                                            <  SRR4302533.3608504/94‑1 (MQ=60)
                                    AACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCG                                                           <  SRR4302533.4103913/94‑1 (MQ=60)
                                    AACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCG                                                           <  SRR4302533.1339103/94‑1 (MQ=60)
                                     ACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGT                                                          >  SRR4302533.2401831/1‑94 (MQ=60)
                                     ACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGT                                                          <  SRR4302533.6319131/94‑1 (MQ=60)
                                      AAGATAGCTGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTCCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTG                                                         <  SRR4302533.3582648/94‑1 (MQ=60)
                                       AGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGT                                                        >  SRR4302533.2969732/1‑94 (MQ=60)
                                       AGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGT                                                        >  SRR4302533.4054271/1‑94 (MQ=60)
                                       AGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGT                                                        >  SRR4302533.3518131/1‑94 (MQ=60)
                                       AGATAGCGGTGCGTTGCTTCGCTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGT                                                        <  SRR4302533.2699582/94‑1 (MQ=60)
                                        GATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTC                                                       >  SRR4302533.5810996/1‑94 (MQ=60)
                                         ATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCG                                                      <  SRR4302533.1491372/94‑1 (MQ=60)
                                         ATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCC                                                      >  SRR4302533.3990052/1‑94 (MQ=60)
                                             CGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAC                                                  <  SRR4302533.4510216/94‑1 (MQ=60)
                                             CGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAC                                                  >  SRR4302533.5075046/1‑94 (MQ=60)
                                              GGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACC                                                 >  SRR4302533.3896489/1‑94 (MQ=60)
                                              GGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACC                                                 >  SRR4302533.2394552/1‑94 (MQ=60)
                                              GGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACC                                                 <  SRR4302533.6328505/94‑1 (MQ=60)
                                                TGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCT                                               >  SRR4302533.3304434/1‑94 (MQ=60)
                                                TGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCT                                               <  SRR4302533.6095983/94‑1 (MQ=60)
                                                 GCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTG                                              <  SRR4302533.5095434/94‑1 (MQ=60)
                                                  CGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGC                                             >  SRR4302533.5884117/1‑94 (MQ=60)
                                                   GTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCA                                            >  SRR4302533.4949299/1‑94 (MQ=60)
                                                   GTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCA                                            <  SRR4302533.1137910/94‑1 (MQ=60)
                                                    TTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAA                                           <  SRR4302533.1134815/94‑1 (MQ=60)
                                                    TTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAA                                           >  SRR4302533.656972/1‑94 (MQ=60)
                                                     TGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAAC                                          <  SRR4302533.5758649/94‑1 (MQ=60)
                                                     TGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAAC                                          >  SRR4302533.5940041/1‑94 (MQ=60)
                                                     TGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAAC                                          >  SRR4302533.3315825/1‑94 (MQ=60)
                                                       CTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA                                        >  SRR4302533.5284673/1‑94 (MQ=60)
                                                       CTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA                                        >  SRR4302533.5108135/1‑94 (MQ=60)
                                                       CTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAA                                        >  SRR4302533.5670687/1‑94 (MQ=60)
                                                        TTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAAT                                       <  SRR4302533.1228459/94‑1 (MQ=60)
                                                        TTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAAT                                       >  SRR4302533.3895646/1‑94 (MQ=60)
                                                        TTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAAT                                       <  SRR4302533.3764227/94‑1 (MQ=60)
                                                         TCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATG                                      <  SRR4302533.4950485/94‑1 (MQ=60)
                                                            TTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGT                                   <  SRR4302533.3924164/94‑1 (MQ=60)
                                                            TTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGT                                   <  SRR4302533.107223/94‑1 (MQ=60)
                                                             TCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTT                                  >  SRR4302533.3716995/1‑94 (MQ=60)
                                                             TCACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTT                                  >  SRR4302533.5251360/1‑94 (MQ=60)
                                                              CACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTC                                 >  SRR4302533.3324890/1‑94 (MQ=60)
                                                               CCCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCA                                <  SRR4302533.6219714/94‑1 (MQ=60)
                                                               ACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCA                                <  SRR4302533.756695/94‑1 (MQ=60)
                                                               ACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCA                                >  SRR4302533.5230594/1‑94 (MQ=60)
                                                               ACCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCA                                <  SRR4302533.5681972/94‑1 (MQ=60)
                                                                CCTCTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCACCAATGAGTTCAG                               >  SRR4302533.3621725/1‑94 (MQ=60)
                                                                  TCTGTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCA                             <  SRR4302533.174499/94‑1 (MQ=60)
                                                                   CTTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAA                            >  SRR4302533.4621990/1‑94 (MQ=60)
                                                                    TTTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAAC                           >  SRR4302533.1063531/1‑94 (MQ=60)
                                                                     TTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACT                          <  SRR4302533.91905/94‑1 (MQ=60)
                                                                     TTCAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACT                          <  SRR4302533.2422923/94‑1 (MQ=60)
                                                                       CAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCA                        >  SRR4302533.484560/1‑94 (MQ=60)
                                                                       CAGCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCA                        <  SRR4302533.4152346/94‑1 (MQ=60)
                                                                         GCGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACG                      <  SRR4302533.2937384/94‑1 (MQ=60)
                                                                          CGCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGC                     >  SRR4302533.1394077/1‑94 (MQ=60)
                                                                           GCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCT                    >  SRR4302533.3874474/1‑94 (MQ=60)
                                                                           GCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCT                    <  SRR4302533.2995094/94‑1 (MQ=60)
                                                                           GCATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGGTCAGCAACTCACGCT                    >  SRR4302533.3874473/1‑94 (MQ=60)
                                                                            CATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTG                   >  SRR4302533.2948173/1‑94 (MQ=60)
                                                                             ATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGA                  >  SRR4302533.343452/1‑94 (MQ=60)
                                                                             ATCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGA                  >  SRR4302533.2876523/1‑94 (MQ=60)
                                                                              TCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAG                 <  SRR4302533.1643937/94‑1 (MQ=60)
                                                                              TCCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAG                 >  SRR4302533.2693537/1‑94 (MQ=60)
                                                                               CCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGT                <  SRR4302533.613625/94‑1 (MQ=60)
                                                                               CCTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGT                >  SRR4302533.4453566/1‑94 (MQ=60)
                                                                                CTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTG               >  SRR4302533.4391804/1‑94 (MQ=60)
                                                                                CTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTG               <  SRR4302533.2178140/94‑1 (MQ=60)
                                                                                CTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTG               <  SRR4302533.4411851/94‑1 (MQ=60)
                                                                                CTCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTG               >  SRR4302533.5014681/1‑94 (MQ=60)
                                                                                 TCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGC              >  SRR4302533.5318764/1‑94 (MQ=60)
                                                                                 TCCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGC              <  SRR4302533.3347434/94‑1 (MQ=60)
                                                                                  CCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCG             <  SRR4302533.2509601/94‑1 (MQ=60)
                                                                                  CCAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCG             >  SRR4302533.1979270/1‑94 (MQ=60)
                                                                                   CAGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGC            >  SRR4302533.4953042/1‑94 (MQ=60)
                                                                                    AGTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCA           >  SRR4302533.5544862/1‑94 (MQ=60)
                                                                                     GTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAG          <  SRR4302533.3019140/94‑1 (MQ=60)
                                                                                     GTTGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAG          >  SRR4302533.1384458/1‑94 (MQ=60)
                                                                                       TGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTT        <  SRR4302533.3837764/94‑1 (MQ=60)
                                                                                       TGCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTT        <  SRR4302533.3938697/94‑1 (MQ=60)
                                                                                        GCCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTG       <  SRR4302533.5932869/94‑1 (MQ=60)
                                                                                         CCCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGT      <  SRR4302533.3918457/94‑1 (MQ=60)
                                                                                          CCGCTGGAGACTTTCAGGTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTG     <  SRR4302533.650131/94‑1 (MQ=60)
                                                                                          CCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTG     <  SRR4302533.890465/94‑1 (MQ=60)
                                                                                          CCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTG     <  SRR4302533.279475/94‑1 (MQ=60)
                                                                                          CCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTG     <  SRR4302533.1708809/94‑1 (MQ=60)
                                                                                          CCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTG     >  SRR4302533.6044960/1‑94 (MQ=60)
                                                                                          CCGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTG     >  SRR4302533.3203269/1‑94 (MQ=60)
                                                                                           CGCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGC    >  SRR4302533.2087399/1‑94 (MQ=60)
                                                                                            GCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCT   <  SRR4302533.2519631/94‑1 (MQ=60)
                                                                                            GCTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCT   >  SRR4302533.4828992/1‑94 (MQ=60)
                                                                                             CTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTT  <  SRR4302533.210075/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTT  >  NC_000913/4385624‑4385810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑