Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,388,932 A→G A52A (GCT→GCC)  b4161 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,388,9320AG99.4% 1092.4 / NA 345A52A (GCT→GCC) b4161hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (164/179);  total (166/179)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 377 total aligned reads displayed.

GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT  >  NC_000913/4388841‑4389021
                                                                                           |                                                                                         
gACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGGTTCCACATCAg                                                                                          >  2:326479/1‑93 (MQ=255)
  cGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCg                                                                                        <  2:1863840/93‑1 (MQ=255)
    ccAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTg                                                                                      >  1:8491390/1‑93 (MQ=255)
    ccAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTg                                                                                      >  1:6469180/1‑93 (MQ=255)
      aGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCa                                                                                    >  2:916551/1‑93 (MQ=255)
      aGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCa                                                                                    >  2:6504867/1‑93 (MQ=255)
       gACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAt                                                                                   >  1:4012591/1‑93 (MQ=255)
          tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGGATAcc                                                                                >  1:2339105/1‑93 (MQ=255)
          tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAcc                                                                                >  1:1821498/1‑93 (MQ=255)
          tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAcc                                                                                <  1:825287/93‑1 (MQ=255)
          tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAcc                                                                                <  2:9252977/93‑1 (MQ=255)
           aCGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCa                                                                               >  1:9634888/1‑93 (MQ=255)
             gcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCaaa                                                                             <  1:697382/93‑1 (MQ=255)
               ggTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGc                                                                           <  2:9608624/93‑1 (MQ=255)
                  cGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGc                                                                        <  2:9063342/93‑1 (MQ=255)
                  cGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGc                                                                        <  1:912759/93‑1 (MQ=255)
                  cGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGc                                                                        <  1:3224793/93‑1 (MQ=255)
                  cGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGc                                                                        <  1:3554278/93‑1 (MQ=255)
                   gCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCt                                                                       <  2:4204153/93‑1 (MQ=255)
                   gCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAACAAAGCGGCt                                                                       >  2:1157496/1‑93 (MQ=255)
                    ccGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGGtg                                                                      >  1:3710933/1‑93 (MQ=255)
                      ggTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgcg                                                                    >  2:5884861/1‑91 (MQ=255)
                       gTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCCAAGCGGCtgatg                                                                   >  2:3058202/1‑93 (MQ=255)
                       gTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCCTGCATACCAAAGCGGctgctg                                                                   >  2:152506/1‑93 (MQ=255)
                        ttACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatga                                                                  <  2:5641257/93‑1 (MQ=255)
                        ttACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatga                                                                  <  1:4078969/93‑1 (MQ=255)
                           ccAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGa                                                               <  2:4029554/93‑1 (MQ=255)
                           ccAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGa                                                               <  2:8647158/93‑1 (MQ=255)
                            cAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAt                                                              >  2:6853430/1‑93 (MQ=255)
                               cGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAcc                                                           >  2:8873692/1‑93 (MQ=255)
                                gAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCt                                                          <  2:5459908/93‑1 (MQ=255)
                                gAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCt                                                          <  2:3715872/93‑1 (MQ=255)
                                gAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCt                                                          <  2:274304/93‑1 (MQ=255)
                                 aaCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCGCATCGGCGTGCATACCAAAGCGGCTGATGACGATACCtt                                                         <  1:1942495/93‑1 (MQ=255)
                                 aaCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCTAAACGGCGGATGGCGATACCtt                                                         >  1:3483709/1‑93 (MQ=255)
                                 aaCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCtt                                                         <  2:4501052/93‑1 (MQ=255)
                                 aaCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCtt                                                         <  2:3662972/93‑1 (MQ=255)
                                  aCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCTGCGTGCATACCAAAACGGCTGATGGCGATACCTtc                                                        >  2:3481647/1‑93 (MQ=255)
                                   cGGGTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGGTGACGGTACGTtcc                                                       >  2:6685171/1‑92 (MQ=255)
                                    ggATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcat                                                      >  1:6630815/1‑93 (MQ=255)
                                     gATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatc                                                     <  2:6135376/93‑1 (MQ=255)
                                     gATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatc                                                     <  2:9680307/93‑1 (MQ=255)
                                      aTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatca                                                    <  1:3071867/93‑1 (MQ=255)
                                      aTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatca                                                    <  2:6229147/93‑1 (MQ=255)
                                       tGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAg                                                   >  1:9782853/1‑93 (MQ=255)
                                       tGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAg                                                   <  1:1450196/93‑1 (MQ=255)
                                        ggTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATCCCTTCATCAgg                                                  >  1:4766153/1‑93 (MQ=255)
                                         gTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGc                                                 <  1:5011213/93‑1 (MQ=255)
                                            cGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCg                                              >  1:7175393/1‑93 (MQ=255)
                                            cGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGGCGATAACTTCATCAGGCTCg                                              >  1:4863503/1‑93 (MQ=255)
                                             ggCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGc                                             >  1:1236410/1‑93 (MQ=255)
                                              gCGAATATTGCAGCGGTTAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGcc                                            >  1:8611348/1‑93 (MQ=255)
                                              gCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGcc                                            >  1:9398562/1‑93 (MQ=255)
                                              gCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGcc                                            >  1:4680315/1‑93 (MQ=255)
                                                gAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCaa                                          >  1:785128/1‑93 (MQ=255)
                                                gAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCACGCTCGCCaa                                          >  2:8257415/1‑93 (MQ=255)
                                                   tatTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACa                                       >  2:2195082/1‑93 (MQ=255)
                                                   tatTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACa                                       >  2:6964338/1‑93 (MQ=255)
                                                    atTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAg                                      >  1:8915116/1‑93 (MQ=255)
                                                     ttGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGa                                     >  1:2208704/1‑93 (MQ=255)
                                                     ttGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGa                                     <  1:2168606/93‑1 (MQ=255)
                                                     ttGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAACTTCATCAGGCTCGCCAAACAGa                                     >  2:5094959/1‑93 (MQ=255)
                                                     tggCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGa                                     <  1:7561067/91‑1 (MQ=255)
                                                      tGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAt                                    <  2:7665395/93‑1 (MQ=255)
                                                        cAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTg                                  >  1:5942269/1‑93 (MQ=255)
                                                         aGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgt                                 <  1:9981444/93‑1 (MQ=255)
                                                           cGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcg                               >  2:5325179/1‑93 (MQ=255)
                                                           cGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcg                               >  2:2337149/1‑93 (MQ=255)
                                                             gTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCCAACAGATTgtcgtc                             >  1:5434115/1‑93 (MQ=255)
                                                             gTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcgtc                             <  2:6362801/93‑1 (MQ=255)
                                                              tGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGtcgtcg                            <  1:112119/93‑1 (MQ=255)
                                                                 aCGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAATGTCGTCGTAg                         >  2:7317810/1‑93 (MQ=255)
                                                                  cGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGt                        <  2:7398105/93‑1 (MQ=255)
                                                                   gTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTc                       <  2:6269713/93‑1 (MQ=255)
                                                                   ctCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTc                       <  2:4850706/92‑1 (MQ=255)
                                                                    tCGCCATCGGCGGATTCCATATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACCGATTGtcgtcgtcgtcg                      >  2:7881258/1‑93 (MQ=255)
                                                                    tCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCg                      >  2:2771718/1‑93 (MQ=255)
                                                                    tCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCg                      >  1:4544996/1‑93 (MQ=255)
                                                                    tCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCg                      >  2:8307626/1‑93 (MQ=255)
                                                                    tCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCg                      >  1:2934770/1‑93 (MQ=255)
                                                                     cGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCgg                     <  1:429665/93‑1 (MQ=255)
                                                                       ccATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGc                   >  1:10041076/1‑93 (MQ=255)
                                                                       ccATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGGCGGGc                   >  1:1639636/1‑93 (MQ=255)
                                                                        cATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCt                  <  2:6884065/93‑1 (MQ=255)
                                                                          tCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGTCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTtc                <  2:2086729/93‑1 (MQ=255)
                                                                           cggcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTtct               <  1:7446439/93‑1 (MQ=255)
                                                                            ggcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTtctc              <  2:7178776/93‑1 (MQ=255)
                                                                             gcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTcc             <  1:4179274/93‑1 (MQ=255)
                                                                             gcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTcc             >  1:6879683/1‑93 (MQ=255)
                                                                              cggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATTCCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCt            <  1:1304144/93‑1 (MQ=255)
                                                                               ggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCACACAGATTGTCGTCGTAGCCGGGCTTCTCCtt           >  1:4476607/1‑93 (MQ=255)
                                                                               ggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTAGGGCTTCTCCtt           >  2:8010377/1‑93 (MQ=255)
                                                                                gATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTa          >  2:2224835/1‑93 (MQ=255)
                                                                                gATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTa          <  1:5794841/93‑1 (MQ=255)
                                                                                 aTTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAg         <  1:2531815/93‑1 (MQ=255)
                                                                                 aTTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAg         >  1:2782097/1‑93 (MQ=255)
                                                                                 aTTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAg         <  1:7266663/93‑1 (MQ=255)
                                                                                  ttCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGa        >  1:8410753/1‑93 (MQ=255)
                                                                                      acaTCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGtt    >  1:2701211/1‑93 (MQ=255)
                                                                                      acaTCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGtt    >  2:2543243/1‑93 (MQ=255)
                                                                                           |                                                                                         
GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT  >  NC_000913/4388841‑4389021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 212 total aligned reads displayed.
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  >  NC_000913/4388839‑4389025
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CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCG                                                                                               <  SRR4302540.5535510/94‑1 (MQ=60)
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCG                                                                                               <  SRR4302540.3331490/94‑1 (MQ=60)
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCG                                                                                               <  SRR4302540.7443489/94‑1 (MQ=60)
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCG                                                                                               >  SRR4302540.9036209/1‑94 (MQ=60)
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCG                                                                                               <  SRR4302540.6999900/94‑1 (MQ=60)
  GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGC                                                                                             <  SRR4302540.3984280/94‑1 (MQ=60)
  GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGC                                                                                             <  SRR4302540.6528017/94‑1 (MQ=60)
  GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGC                                                                                             <  SRR4302540.5184732/94‑1 (MQ=60)
   ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCG                                                                                            <  SRR4302540.6817292/94‑1 (MQ=60)
     GCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTG                                                                                          >  SRR4302540.6541160/1‑94 (MQ=60)
     GCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTG                                                                                          >  SRR4302540.8590052/1‑94 (MQ=60)
       CAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCT                                                                                        >  SRR4302540.6542/1‑94 (MQ=60)
       CAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCA                                                                                        <  SRR4302540.660479/94‑1 (MQ=60)
        AGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAT                                                                                       >  SRR4302540.4055806/1‑94 (MQ=60)
        AGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAT                                                                                       >  SRR4302540.7076449/1‑94 (MQ=60)
         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATA                                                                                      <  SRR4302540.1103357/94‑1 (MQ=60)
         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCACA                                                                                      <  SRR4302540.2745916/94‑1 (MQ=60)
           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGGATACC                                                                                    >  SRR4302540.2364311/1‑94 (MQ=60)
           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACC                                                                                    >  SRR4302540.6160535/1‑94 (MQ=60)
           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACC                                                                                    >  SRR4302540.1841400/1‑94 (MQ=60)
            TACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCA                                                                                   >  SRR4302540.9748403/1‑94 (MQ=60)
            TACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCA                                                                                   <  SRR4302540.834066/94‑1 (MQ=60)
            TACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCA                                                                                   <  SRR4302540.2254100/94‑1 (MQ=60)
              CGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAA                                                                                 <  SRR4302540.7682725/94‑1 (MQ=60)
              AGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAA                                                                                 <  SRR4302540.1571316/94‑1 (MQ=60)
               GCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAG                                                                                <  SRR4302540.704862/94‑1 (MQ=60)
                 GGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCCGCGGTGAACGGCGCCATCGGCGGGATCCACATCGGCGTGCATACCAcaacg                                                                              >  SRR4302540.5542667/1‑89 (MQ=60)
                 GGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCG                                                                              <  SRR4302540.2633175/94‑1 (MQ=60)
                 GGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCG                                                                              >  SRR4302540.1757155/1‑94 (MQ=60)
                 GGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCG                                                                              >  SRR4302540.1059569/1‑94 (MQ=60)
                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                           >  SRR4302540.3777792/1‑94 (MQ=60)
                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                           <  SRR4302540.3593113/94‑1 (MQ=60)
                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                           <  SRR4302540.922417/94‑1 (MQ=60)
                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                           <  SRR4302540.3259848/94‑1 (MQ=60)
                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                           <  SRR4302540.10005525/94‑1 (MQ=60)
                     GCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGGTG                                                                          >  SRR4302540.3751430/1‑94 (MQ=60)
                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                        <  SRR4302540.9233631/94‑1 (MQ=60)
                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                        >  SRR4302540.3559102/1‑94 (MQ=60)
                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                        <  SRR4302540.4952121/94‑1 (MQ=60)
                        gtttgccggcgagcgcgttgttcagccAATATTGCAGCGGTGTACGGGTCCATCGGCTGATTCCACATCGGCGTGCATACCAAAGCGGCTGATG                                                                       <  SRR4302540.884200/67‑1 (MQ=38)
                        GGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATG                                                                       <  SRR4302540.7450632/94‑1 (MQ=60)
                        GGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATG                                                                       <  SRR4302540.884201/94‑1 (MQ=60)
                        GGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATG                                                                       <  SRR4302540.7275573/94‑1 (MQ=60)
                        GGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATG                                                                       >  SRR4302540.2881581/1‑94 (MQ=60)
                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAC                                                                     <  SRR4302540.4122953/94‑1 (MQ=60)
                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAC                                                                     <  SRR4302540.4664398/94‑1 (MQ=60)
                           TACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACG                                                                    <  SRR4302540.8687751/94‑1 (MQ=60)
                            ACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCCAATCAGCGGGCATACCAAAGCGGCTGATGACAA                                                                   >  SRR4302540.1759738/1‑94 (MQ=60)
                             CCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAT                                                                  <  SRR4302540.2480005/94‑1 (MQ=60)
                             CCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAT                                                                  <  SRR4302540.5534360/94‑1 (MQ=60)
                             CCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAT                                                                  <  SRR4302540.7315110/94‑1 (MQ=60)
                                GCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACC                                                               <  SRR4302540.4340627/94‑1 (MQ=60)
                                GCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACC                                                               <  SRR4302540.7206339/94‑1 (MQ=60)
                                GCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACC                                                               <  SRR4302540.3082747/94‑1 (MQ=60)
                                 CGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCT                                                              <  SRR4302540.8195097/94‑1 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAATGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGCCTGATGACGACACCTC                                                             >  SRR4302540.2131523/1‑94 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCTAAACGGCGGATGGCGATACCTT                                                             >  SRR4302540.3521634/1‑94 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTT                                                             >  SRR4302540.5380345/1‑94 (MQ=60)
                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTT                                                             >  SRR4302540.8565043/1‑94 (MQ=60)
                                   AACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCGCATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTC                                                            <  SRR4302540.1963644/94‑1 (MQ=60)
                                    ACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCA                                                           <  SRR4302540.8657872/94‑1 (MQ=60)
                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                          >  SRR4302540.6704212/1‑94 (MQ=60)
                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                          >  SRR4302540.5923087/1‑94 (MQ=60)
                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                          >  SRR4302540.4913771/1‑94 (MQ=60)
                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                          >  SRR4302540.4565438/1‑94 (MQ=60)
                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                          <  SRR4302540.8951262/94‑1 (MQ=60)
                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                          <  SRR4302540.4231417/94‑1 (MQ=60)
                                       GATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCA                                                        >  SRR4302540.7427812/1‑94 (MQ=60)
                                       GATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCA                                                        >  SRR4302540.6209664/1‑94 (MQ=60)
                                       GATGGGACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCA                                                        <  SRR4302540.8572148/94‑1 (MQ=60)
                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAG                                                       <  SRR4302540.3350624/94‑1 (MQ=60)
                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAG                                                       >  SRR4302540.9897497/1‑94 (MQ=60)
                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAG                                                       >  SRR4302540.9897496/1‑94 (MQ=60)
                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAG                                                       <  SRR4302540.3105571/94‑1 (MQ=60)
                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACAAAAGCGGCTGATGACGATACCTTCATCAG                                                       >  SRR4302540.22142/1‑94 (MQ=60)
                                         TGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATCCCTTCATCAGG                                                      >  SRR4302540.4817047/1‑94 (MQ=60)
                                         TGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGG                                                      <  SRR4302540.1465963/94‑1 (MQ=60)
                                          GGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGC                                                     <  SRR4302540.447185/94‑1 (MQ=60)
                                          GGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGC                                                     >  SRR4302540.7703891/1‑94 (MQ=60)
                                           GTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCT                                                    <  SRR4302540.5065473/94‑1 (MQ=60)
                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCTACATCGGCGTGCATACCAAAGCGGCTGATGAAGATACCTTCAgcagggtag                                                  >  SRR4302540.4915659/1‑85 (MQ=60)
                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCCCATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCG                                                  >  SRR4302540.4915661/1‑94 (MQ=60)
                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCG                                                  >  SRR4302540.5923477/1‑94 (MQ=60)
                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCG                                                  <  SRR4302540.3177859/94‑1 (MQ=60)
                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCG                                                  >  SRR4302540.7255377/1‑94 (MQ=60)
                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGGCGATAACTTCATCAGGCTCG                                                  >  SRR4302540.4915663/1‑94 (MQ=60)
                                              CGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCcagatcgc                                                 >  SRR4302540.1249037/1‑86 (MQ=60)
                                              CGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGC                                                 >  SRR4302540.1249036/1‑94 (MQ=60)
                                               GGCGAATATTGCAGCGGTTAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCC                                                >  SRR4302540.8711406/1‑94 (MQ=60)
                                               GGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTTATCAGGCTCGCC                                                >  SRR4302540.3185301/1‑94 (MQ=60)
                                               GGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCC                                                >  SRR4302540.9510382/1‑94 (MQ=60)
                                                 CGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCCA                                              >  SRR4302540.4521721/1‑94 (MQ=60)
                                                 CGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAA                                              >  SRR4302540.793474/1‑94 (MQ=60)
                                                  GAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAA                                             >  SRR4302540.8441436/1‑94 (MQ=60)
                                                   AATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAAC                                            <  SRR4302540.3991070/94‑1 (MQ=60)
                                                     TATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAG                                          >  SRR4302540.9018361/1‑94 (MQ=60)
                                                      ATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGA                                         >  SRR4302540.2232561/1‑94 (MQ=60)
                                                       TTTCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACGTTCATCAGGCTCGCCAAACAGAT                                        <  SRR4302540.1844433/94‑1 (MQ=60)
                                                       TTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAT                                        <  SRR4302540.2191990/94‑1 (MQ=60)
                                                       TTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAT                                        <  SRR4302540.1310922/94‑1 (MQ=60)
                                                       TTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAT                                        <  SRR4302540.7645871/94‑1 (MQ=60)
                                                       TGGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAT                                        <  SRR4302540.7645872/94‑1 (MQ=60)
                                                        TGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATT                                       <  SRR4302540.4671287/94‑1 (MQ=60)
                                                        TGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATT                                       >  SRR4302540.4146298/1‑94 (MQ=60)
                                                         GCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTG                                      >  SRR4302540.6007960/1‑94 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCTTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    <  SRR4302540.1139891/94‑1 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    <  SRR4302540.1139893/94‑1 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    >  SRR4302540.1244304/1‑94 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    <  SRR4302540.3613480/94‑1 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    >  SRR4302540.2702572/1‑94 (MQ=60)
                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    <  SRR4302540.10097830/94‑1 (MQ=60)
                                                           AGCGGAGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                    <  SRR4302540.3489793/94‑1 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCCAACAGATTGTCGTC                                 >  SRR4302540.5493882/1‑94 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 <  SRR4302540.3175202/94‑1 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 >  SRR4302540.7000209/1‑94 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 <  SRR4302540.6434732/94‑1 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 <  SRR4302540.4016031/94‑1 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 <  SRR4302540.2341130/94‑1 (MQ=60)
                                                              GGTGAACGTCGCCATCGGCGGATGCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATGCCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                 <  SRR4302540.4071094/94‑1 (MQ=60)
                                                               GTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCG                                >  SRR4302540.3677439/1‑94 (MQ=60)
                                                                TGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGGCAAACAGATTGTCGTCGT                               >  SRR4302540.5039100/1‑94 (MQ=60)
                                                                TGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGT                               <  SRR4302540.113314/94‑1 (MQ=60)
                                                                  AACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAG                             <  SRR4302540.8108010/94‑1 (MQ=60)
                                                                  AACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAG                             <  SRR4302540.306803/94‑1 (MQ=60)
                                                                  AACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAG                             <  SRR4302540.2164378/94‑1 (MQ=60)
                                                                  AACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAACTTCATCAGGCTCGCAAAACAGATTGTCGTCGTAG                             >  SRR4302540.1848693/1‑94 (MQ=60)
                                                                   ACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGT                            <  SRR4302540.1501308/94‑1 (MQ=60)
                                                                    CGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTC                           <  SRR4302540.9883655/94‑1 (MQ=60)
                                                                    CGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTC                           >  SRR4302540.1812202/1‑94 (MQ=60)
                                                                    CGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTC                           <  SRR4302540.4738296/94‑1 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          <  SRR4302540.3072074/94‑1 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          <  SRR4302540.7482455/94‑1 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          >  SRR4302540.4594179/1‑94 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          >  SRR4302540.5108885/1‑94 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                          >  SRR4302540.2966974/1‑94 (MQ=60)
                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGACTGTCGTCGTAGGCG                          >  SRR4302540.2966976/1‑94 (MQ=60)
                                                                      TCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGG                         <  SRR4302540.4797283/94‑1 (MQ=60)
                                                                      TCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGG                         <  SRR4302540.2308692/94‑1 (MQ=60)
                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                        >  SRR4302540.979741/1‑94 (MQ=60)
                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                        >  SRR4302540.8000366/1‑94 (MQ=60)
                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                        <  SRR4302540.434186/94‑1 (MQ=60)
                                                                        GCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGC                       >  SRR4302540.1962669/1‑94 (MQ=60)
                                                                        GCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGC                       >  SRR4302540.10157826/1‑94 (MQ=60)
                                                                        GCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGGCGGGC                       >  SRR4302540.1657557/1‑94 (MQ=60)
                                                                          CATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTT                     >  SRR4302540.10089143/1‑94 (MQ=60)
                                                                           CTCGGCGGATTCCACATCGGCGTGCATACCAAAGCTGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                    <  SRR4302540.6957962/94‑1 (MQ=60)
                                                                           ATCGGCGGATTCCCCATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                    >  SRR4302540.2515708/1‑94 (MQ=60)
                                                                           ATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                    >  SRR4302540.9910628/1‑94 (MQ=60)
                                                                           ATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                    >  SRR4302540.1264318/1‑94 (MQ=60)
                                                                           ATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                    >  SRR4302540.931413/1‑94 (MQ=60)
                                                                           ATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                    <  SRR4302540.9000607/94‑1 (MQ=60)
                                                                           ATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACATTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                    >  SRR4302540.7615625/1‑94 (MQ=60)
                                                                            TCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCT                   <  SRR4302540.2811667/94‑1 (MQ=60)
                                                                             CGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTC                  <  SRR4302540.7529406/94‑1 (MQ=60)
                                                                             CGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTC                  >  SRR4302540.4877209/1‑94 (MQ=60)
                                                                              GGTGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCC                 >  SRR4302540.2088602/1‑94 (MQ=60)
                                                                              GGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCC                 >  SRR4302540.6955939/1‑94 (MQ=60)
                                                                              GGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCC                 >  SRR4302540.2949886/1‑94 (MQ=60)
                                                                               GCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCT                <  SRR4302540.7029095/94‑1 (MQ=60)
                                                                               GCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCT                <  SRR4302540.4224656/94‑1 (MQ=60)
                                                                                GGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTT               <  SRR4302540.1317675/94‑1 (MQ=60)
                                                                                CGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATTCCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTT               <  SRR4302540.1317676/94‑1 (MQ=60)
                                                                                CGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCACACAGATTGTCGTCGTAGCCGGGCTTCTCCTT               >  SRR4302540.4525166/1‑94 (MQ=60)
                                                                                CGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGCTTGTCGTCGTAGTCGGGTTTGTCCTT               >  SRR4302540.4525168/1‑94 (MQ=60)
                                                                                CGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTT               >  SRR4302540.4525167/1‑94 (MQ=60)
                                                                                CGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTT               <  SRR4302540.4344326/94‑1 (MQ=60)
                                                                                 GGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGACGTCGTAGTCGGGCTTCTCCTTA              >  SRR4302540.9080028/1‑94 (MQ=60)
                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG             <  SRR4302540.5858874/94‑1 (MQ=60)
                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG             >  SRR4302540.9320386/1‑94 (MQ=60)
                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG             >  SRR4302540.2812151/1‑94 (MQ=60)
                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG             >  SRR4302540.3676109/1‑94 (MQ=60)
                                                                                  GATGCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG             <  SRR4302540.6711392/94‑1 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            <  SRR4302540.2559131/94‑1 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            <  SRR4302540.9574143/94‑1 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            >  SRR4302540.4092908/1‑94 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            >  SRR4302540.4092909/1‑94 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            >  SRR4302540.9645863/1‑94 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            >  SRR4302540.8508375/1‑94 (MQ=60)
                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA            >  SRR4302540.8492884/1‑94 (MQ=60)
                                                                                    TTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAC           >  SRR4302540.7079541/1‑94 (MQ=60)
                                                                                    TTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAC           >  SRR4302540.8622838/1‑94 (MQ=60)
                                                                                     TCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACG          >  SRR4302540.4230021/1‑94 (MQ=60)
                                                                                     TCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACG          <  SRR4302540.1371452/94‑1 (MQ=60)
                                                                                      CCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGT         >  SRR4302540.728065/1‑94 (MQ=60)
                                                                                      CCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGT         >  SRR4302540.5920748/1‑94 (MQ=60)
                                                                                       CACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTT        >  SRR4302540.5288050/1‑94 (MQ=60)
                                                                                       CACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTT        >  SRR4302540.7768386/1‑94 (MQ=60)
                                                                                       CACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTT        <  SRR4302540.4614171/94‑1 (MQ=60)
                                                                                       CACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTT        <  SRR4302540.1464648/94‑1 (MQ=60)
                                                                                       CACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTT        <  SRR4302540.5059170/94‑1 (MQ=60)
                                                                                       CACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTT        >  SRR4302540.2730359/1‑94 (MQ=60)
                                                                                        CCATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTT       <  SRR4302540.2707101/94‑1 (MQ=60)
                                                                                        ACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTT       >  SRR4302540.959242/1‑94 (MQ=60)
                                                                                         CATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT      >  SRR4302540.2512650/1‑94 (MQ=60)
                                                                                          ATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTA     >  SRR4302540.908566/1‑94 (MQ=60)
                                                                                            CGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAG   <  SRR4302540.5663256/94‑1 (MQ=60)
                                                                                             GGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTTGGGCTTCTCCTTAGACCTTTTAAGA  >  SRR4302540.5829292/1‑94 (MQ=60)
                                                                                             GGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  >  SRR4302540.93360/1‑94 (MQ=60)
                                                                                             GGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  <  SRR4302540.2395182/94‑1 (MQ=60)
                                                                                             GGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGATTCTCCTTAGACGTTTTCAGA  >  SRR4302540.9237493/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGA  >  NC_000913/4388839‑4389025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑