Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,388,932	A→G	A52A (GCT→GCC)	b4161 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,388,932	0	A	G	99.6%	694.5 / NA	221	A52A (GCT→GCC)	b4161	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (105/114); minor base C (1/0); total (107/114)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT  >  NC_000913/4388842‑4389021

aCGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGc                                                                                         >  2:5490476/1‑93 (MQ=255)

aCGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGc                                                                                         >  1:760985/1‑93 (MQ=255)

 cGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCg                                                                                        <  2:6054038/93‑1 (MQ=255)

  gCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGt                                                                                       <  1:6192753/93‑1 (MQ=255)

    cAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGc                                                                                     >  1:4327695/1‑93 (MQ=255)

    cAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGc                                                                                     >  2:5539778/1‑93 (MQ=255)

      gACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCTGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAt                                                                                   <  2:2107840/93‑1 (MQ=255)

      gACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCAt                                                                                   <  1:2866626/93‑1 (MQ=255)

       aCTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATa                                                                                  >  1:3846816/1‑93 (MQ=255)

       aCTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATa                                                                                  >  1:1725802/1‑93 (MQ=255)

       aCTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATa                                                                                  <  1:639615/93‑1 (MQ=255)

        cTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAc                                                                                 <  1:5073347/93‑1 (MQ=255)

         tACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAcc                                                                                >  1:4035217/1‑93 (MQ=255)

          aCGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCa                                                                               >  2:737771/1‑93 (MQ=255)

           cgcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCaa                                                                              <  2:1051923/93‑1 (MQ=255)

           cgcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCaa                                                                              <  2:4242240/93‑1 (MQ=255)

           cgcgGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGGGCATACCaa                                                                              >  1:2612133/1‑93 (MQ=255)

              ggTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGc                                                                           <  2:5783128/93‑1 (MQ=255)

               gTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCg                                                                          <  1:2208140/93‑1 (MQ=255)

               gTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAACAAAGCg                                                                          >  2:175726/1‑93 (MQ=255)

                tCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCgg                                                                         >  1:5914655/1‑93 (MQ=255)

                 cGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGc                                                                        <  1:1340449/93‑1 (MQ=255)

                 cGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGc                                                                        <  2:4503158/93‑1 (MQ=255)

                   ccGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtg                                                                      >  2:3903585/1‑93 (MQ=255)

                    cGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtga                                                                     <  1:5528069/93‑1 (MQ=255)

                     ggTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgat                                                                    <  2:5372259/93‑1 (MQ=255)

                      gTTGCCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatg                                                                   <  1:5504032/93‑1 (MQ=255)

                      gTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatg                                                                   <  1:1877452/93‑1 (MQ=255)

                       ttACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCtgatga                                                                  <  2:1296232/93‑1 (MQ=255)

                        tACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAc                                                                 >  1:2097538/1‑93 (MQ=255)

                        tACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAc                                                                 <  1:5158095/93‑1 (MQ=255)

                          ccAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGa                                                               <  2:815324/93‑1 (MQ=255)

                           cAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAt                                                              <  2:5353992/93‑1 (MQ=255)

                           cAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAt                                                              >  2:3228917/1‑93 (MQ=255)

                           cAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAAGAt                                                              >  1:4652806/1‑93 (MQ=255)

                            aGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATa                                                             <  2:2233248/93‑1 (MQ=255)

                             gCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAc                                                            >  1:2960088/1‑93 (MQ=255)

                              cGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAGAcc                                                           >  1:5261189/1‑93 (MQ=255)

                                aaCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCtt                                                         >  2:5820547/1‑93 (MQ=255)

                                aaCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCtt                                                         <  1:4278178/93‑1 (MQ=255)

                                 aCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtc                                                        <  2:2167902/93‑1 (MQ=255)

                                 aCGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtc                                                        <  1:737771/93‑1 (MQ=255)

                                  cGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtca                                                       <  1:5980569/93‑1 (MQ=255)

                                    gATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTtcatc                                                     <  1:3446849/93‑1 (MQ=255)

                                       ggTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAgg                                                  <  1:423462/93‑1 (MQ=255)

                                       ggTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAgg                                                  <  1:3080325/93‑1 (MQ=255)

                                        gTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGc                                                 >  1:5889129/1‑93 (MQ=255)

                                        gTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGc                                                 <  2:2935237/93‑1 (MQ=255)

                                        gTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGc                                                 <  2:2598534/93‑1 (MQ=255)

                                         tACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCt                                                <  2:4037633/93‑1 (MQ=255)

                                         tACGGCGAATATTGCAGCGGGGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGAGGCTGAACACCAGACCGTCACTAGGCt                                                >  1:4866051/1‑93 (MQ=255)

                                          aCGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTc                                               >  2:5432372/1‑93 (MQ=255)

                                           cGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCg                                              >  2:1855312/1‑93 (MQ=255)

                                            ggCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGc                                             >  1:5431409/1‑93 (MQ=255)

                                             gCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGcc                                            >  2:1959271/1‑93 (MQ=255)

                                             gCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGcc                                            <  1:4185865/93‑1 (MQ=255)

                                             gCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGcc                                            >  2:3440996/1‑93 (MQ=255)

                                               gAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCaa                                          >  1:3295436/1‑93 (MQ=255)

                                                aaTATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCaaa                                         >  1:4811888/1‑93 (MQ=255)

                                                 atatTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAAc                                        >  2:4577958/1‑93 (MQ=255)

                                                 atatTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAAc                                        <  1:476622/93‑1 (MQ=255)

                                                   atTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCCCCAAACAg                                      >  1:4535212/1‑93 (MQ=255)

                                                    ttGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGa                                     <  1:1386465/93‑1 (MQ=255)

                                                     tGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAt                                    <  1:3221429/93‑1 (MQ=255)

                                                      gCAGCGGTCAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAAGACGATACCTTCATCAGGCTCGACAAACACAtt                                   >  2:6137844/1‑93 (MQ=255)

                                                        aGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgt                                 <  2:1615792/93‑1 (MQ=255)

                                                         gCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtc                                <  2:2377119/93‑1 (MQ=255)

                                                           ggTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATTAAGATACCGTCATCAGGCTCGCCAAACAGATTgtcgt                              >  2:2535739/1‑93 (MQ=255)

                                                           ggTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcgt                              <  1:3420220/93‑1 (MQ=255)

                                                            gTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcgtc                             <  1:884211/93‑1 (MQ=255)

                                                            gTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcgtc                             <  2:193045/93‑1 (MQ=255)

                                                            gTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTgtcgtc                             >  2:1804866/1‑93 (MQ=255)

                                                               aaCGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTa                          <  1:4940043/93‑1 (MQ=255)

                                                               aaCGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAGGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTa                          <  1:4439781/93‑1 (MQ=255)

                                                                 cGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGt                        <  2:4409805/93‑1 (MQ=255)

                                                                 cGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGt                        <  2:488834/93‑1 (MQ=255)

                                                                 cGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGt                        >  1:6221329/1‑93 (MQ=255)

                                                                 cGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGt                        <  2:2736243/93‑1 (MQ=255)

                                                                  gTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAACTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTc                       >  2:596560/1‑93 (MQ=255)

                                                                     gCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCggg                    >  1:6293840/1‑93 (MQ=255)

                                                                     gCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCggg                    >  2:5474999/1‑93 (MQ=255)

                                                                     gCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCggg                    >  1:479304/1‑93 (MQ=255)

                                                                      ccATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGc                   >  1:5854863/1‑93 (MQ=255)

                                                                       cATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCt                  >  2:5160441/1‑93 (MQ=255)

                                                                       cATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCt                  >  1:2387617/1‑93 (MQ=255)

                                                                       cATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTAGTCGTAGTCGGGCt                  >  1:2791443/1‑93 (MQ=255)

                                                                         tCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTtc                >  2:4657493/1‑93 (MQ=255)

                                                                         tCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTtc                <  1:1235884/93‑1 (MQ=255)

                                                                            gcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTcc             <  2:5550876/93‑1 (MQ=255)

                                                                            gcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTcc             >  2:5803149/1‑93 (MQ=255)

                                                                            gcggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTcc             <  1:6083737/93‑1 (MQ=255)

                                                                              ggATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCtt           >  1:2908828/1‑93 (MQ=255)

                                                                               gATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTa          <  1:1083281/93‑1 (MQ=255)

                                                                                 ttCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGa        <  2:3782895/93‑1 (MQ=255)

                                                                                 ttCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGa        >  2:1118278/1‑93 (MQ=255)

                                                                                 ttCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGa        <  2:5358802/93‑1 (MQ=255)

                                                                                  tCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGTTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAc       <  2:4111167/93‑1 (MQ=255)

                                                                                  tCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGAc       >  2:1212881/1‑93 (MQ=255)

                                                                                      caTCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGttt   <  1:3714109/93‑1 (MQ=255)

                                                                                       aTCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGtttt  >  1:5532093/1‑93 (MQ=255)

ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT  >  NC_000913/4388842‑4389021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAG  >  NC_000913/4388839‑4389024

CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCG                                                                                              >  SRR4302533.2824855/1‑94 (MQ=60)

  GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGC                                                                                            >  SRR4302533.765462/1‑94 (MQ=60)

  GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGC                                                                                            <  SRR4302533.4843007/94‑1 (MQ=60)

  GACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGC                                                                                            <  SRR4302533.4979970/94‑1 (MQ=60)

   ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCG                                                                                           >  SRR4302533.864830/1‑94 (MQ=60)

   ACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCG                                                                                           <  SRR4302533.6151565/94‑1 (MQ=60)

     GCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTG                                                                                         <  SRR4302533.6229914/94‑1 (MQ=60)

      CCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGC                                                                                        >  SRR4302533.4353764/1‑94 (MQ=60)

       CAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCA                                                                                       <  SRR4302533.1349525/94‑1 (MQ=60)

       CAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCA                                                                                       >  SRR4302533.2986558/1‑94 (MQ=60)

         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATA                                                                                     >  SRR4302533.3870307/1‑94 (MQ=60)

         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATA                                                                                     <  SRR4302533.2884262/94‑1 (MQ=60)

         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATA                                                                                     >  SRR4302533.718138/1‑94 (MQ=60)

         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATA                                                                                     >  SRR4302533.515531/1‑94 (MQ=60)

         GACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATA                                                                                     >  SRR4302533.1736275/1‑94 (MQ=60)

          ACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATAC                                                                                    <  SRR4302533.643379/94‑1 (MQ=60)

           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACC                                                                                   <  SRR4302533.5104013/94‑1 (MQ=60)

           CTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACC                                                                                   >  SRR4302533.4059781/1‑94 (MQ=60)

            TACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCA                                                                                  <  SRR4302533.6266837/94‑1 (MQ=60)

             ACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGGGCATACCAA                                                                                 >  SRR4302533.2628165/1‑94 (MQ=60)

              CGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAA                                                                                >  SRR4302533.2817104/1‑94 (MQ=60)

               GCGGTCGCCGGTTACCAGCGAACGGATGCTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAG                                                                               <  SRR4302533.6096883/94‑1 (MQ=60)

                CGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGC                                                                              <  SRR4302533.4931419/94‑1 (MQ=60)

                CGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGC                                                                              <  SRR4302533.2970221/94‑1 (MQ=60)

                  GTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGG                                                                            <  SRR4302533.2221760/94‑1 (MQ=60)

                  GTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGG                                                                            >  SRR4302533.5950398/1‑94 (MQ=60)

                   TCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGC                                                                           <  SRR4302533.2911797/94‑1 (MQ=60)

                    CGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCT                                                                          <  SRR4302533.1348517/94‑1 (MQ=60)

                     GCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTG                                                                         <  SRR4302533.4980652/94‑1 (MQ=60)

                     GCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTG                                                                         <  SRR4302533.5933876/94‑1 (MQ=60)

                      CCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGAATCCACAACGGCGTGCATACCAAAGCGGCTGA                                                                        >  SRR4302533.6333156/1‑94 (MQ=60)

                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                       >  SRR4302533.181306/1‑94 (MQ=60)

                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                       >  SRR4302533.2710862/1‑94 (MQ=60)

                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                       <  SRR4302533.5969757/94‑1 (MQ=60)

                       CGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAT                                                                       <  SRR4302533.5561446/94‑1 (MQ=60)

                        GGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCTCATCGGCGTGCAAACCAACGAGGGTGATG                                                                      >  SRR4302533.5179511/1‑94 (MQ=60)

                         GTTGCCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                     <  SRR4302533.5537240/94‑1 (MQ=60)

                         GTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGA                                                                     <  SRR4302533.1888895/94‑1 (MQ=60)

                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAC                                                                    >  SRR4302533.2110451/1‑94 (MQ=60)

                          TTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAC                                                                    <  SRR4302533.3379521/94‑1 (MQ=60)

                           TACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACG                                                                   <  SRR4302533.5189233/94‑1 (MQ=60)

                            ACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGA                                                                  <  SRR4302533.1587864/94‑1 (MQ=60)

                             CCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGAAGAT                                                                 >  SRR4302533.4681001/1‑94 (MQ=60)

                              CAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGCTA                                                                >  SRR4302533.1338445/1‑94 (MQ=60)

                              CAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATA                                                                <  SRR4302533.754919/94‑1 (MQ=60)

                               AGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATAC                                                               >  SRR4302533.2978180/1‑94 (MQ=60)

                                GCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACC                                                              >  SRR4302533.4893982/1‑94 (MQ=60)

                                GCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGAGACC                                                              >  SRR4302533.5292775/1‑94 (MQ=60)

                                GCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCCGATGACGATACC                                                              >  SRR4302533.5292777/1‑94 (MQ=60)

                                  GAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTT                                                            <  SRR4302533.3189095/94‑1 (MQ=60)

                                   AACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTC                                                           <  SRR4302533.4303904/94‑1 (MQ=60)

                                    ACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCA                                                          <  SRR4302533.742115/94‑1 (MQ=60)

                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                         <  SRR4302533.6016791/94‑1 (MQ=60)

                                     CGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCAT                                                         >  SRR4302533.2303703/1‑94 (MQ=60)

                                      GGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATC                                                        >  SRR4302533.5469537/1‑94 (MQ=60)

                                       GATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCA                                                       <  SRR4302533.3468133/94‑1 (MQ=60)

                                        ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAG                                                      >  SRR4302533.5562911/1‑94 (MQ=60)

                                         TGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGG                                                     <  SRR4302533.3836576/94‑1 (MQ=60)

                                         TGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGG                                                     <  SRR4302533.4960635/94‑1 (MQ=60)

                                          GGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGC                                                    <  SRR4302533.425995/94‑1 (MQ=60)

                                          GGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGC                                                    <  SRR4302533.3099223/94‑1 (MQ=60)

                                          GGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGC                                                    <  SRR4302533.2842230/94‑1 (MQ=60)

                                          GGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGC                                                    >  SRR4302533.5924710/1‑94 (MQ=60)

                                           GTACGGCGAATATTGCAGCGGGGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGAGGCTGAacaccagaccgtcactaggct                                                   >  SRR4302533.4895540/1‑73 (MQ=60)

                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTTG                                                 >  SRR4302533.4060419/1‑94 (MQ=60)

                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCG                                                 >  SRR4302533.3252670/1‑94 (MQ=60)

                                             ACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCG                                                 >  SRR4302533.4048210/1‑94 (MQ=60)

                                              CGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGC                                                >  SRR4302533.5464118/1‑94 (MQ=60)

                                               GGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCC                                               >  SRR4302533.578646/1‑94 (MQ=60)

                                                GCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCA                                              <  SRR4302533.4211210/94‑1 (MQ=60)

                                                GCGAATATTGAAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATCACAAAGCGGCTGCTGACGATACCTTCATCAGGCTCCCCA                                              >  SRR4302533.2221078/1‑94 (MQ=60)

                                                 CGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAA                                             >  SRR4302533.3315778/1‑94 (MQ=60)

                                                  GAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAA                                            >  SRR4302533.2008023/1‑94 (MQ=60)

                                                  GAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAA                                            >  SRR4302533.4841051/1‑94 (MQ=60)

                                                   AATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGGTACCTTCATCAGGCTCGCCAAAC                                           >  SRR4302533.3013798/1‑94 (MQ=60)

                                                    ATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACA                                          <  SRR4302533.479467/94‑1 (MQ=60)

                                                     TATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAG                                         <  SRR4302533.1901324/94‑1 (MQ=60)

                                                     TATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCCCCAAACAG                                         >  SRR4302533.4562672/1‑94 (MQ=60)

                                                       TTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGAT                                       <  SRR4302533.1394808/94‑1 (MQ=60)

                                                        TGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATT                                      <  SRR4302533.3241294/94‑1 (MQ=60)

                                                        TGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATT                                      >  SRR4302533.5897234/1‑94 (MQ=60)

                                                         GCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACGGATTG                                     >  SRR4302533.4797344/1‑94 (MQ=60)

                                                         GCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTG                                     <  SRR4302533.5969224/94‑1 (MQ=60)

                                                         GCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTG                                     <  SRR4302533.3562743/94‑1 (MQ=60)

                                                          CAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGT                                    <  SRR4302533.4332441/94‑1 (MQ=60)

                                                          CAGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGT                                    <  SRR4302533.428789/94‑1 (MQ=60)

                                                           AGCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTC                                   >  SRR4302533.3455789/1‑94 (MQ=60)

                                                            GCGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCG                                  <  SRR4302533.4392910/94‑1 (MQ=60)

                                                             CGGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGT                                 <  SRR4302533.5196461/94‑1 (MQ=60)

                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                <  SRR4302533.4563429/94‑1 (MQ=60)

                                                              GGTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTC                                <  SRR4302533.3441352/94‑1 (MQ=60)

                                                               GTGAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCG                               <  SRR4302533.889403/94‑1 (MQ=60)

                                                                 GAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTA                             >  SRR4302533.3380638/1‑94 (MQ=60)

                                                                 GAACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTA                             <  SRR4302533.4570656/94‑1 (MQ=60)

                                                                  AACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAG                            >  SRR4302533.4247650/1‑94 (MQ=60)

                                                                  AACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAG                            <  SRR4302533.4969950/94‑1 (MQ=60)

                                                                  AACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGAGGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAG                            <  SRR4302533.4466618/94‑1 (MQ=60)

                                                                   ACGTCGCCATCGGCGGATTCCACATCGGCGTGCATCCCAAAGCGGCTGATGACGATGCCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGT                           <  SRR4302533.4064727/94‑1 (MQ=60)

                                                                   ACGTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGT                           >  SRR4302533.6258620/1‑94 (MQ=60)

                                                                     GTCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCG                         <  SRR4302533.123478/94‑1 (MQ=60)

                                                                      TCGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGG                        <  SRR4302533.4801200/94‑1 (MQ=60)

                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                       >  SRR4302533.6331360/1‑94 (MQ=60)

                                                                       CGCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGG                       >  SRR4302533.482168/1‑94 (MQ=60)

                                                                        GCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGC                      >  SRR4302533.5890260/1‑94 (MQ=60)

                                                                        GCCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGC                      <  SRR4302533.4330978/94‑1 (MQ=60)

                                                                         CCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCT                     <  SRR4302533.4690994/94‑1 (MQ=60)

                                                                         CCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCT                     >  SRR4302533.2402367/1‑94 (MQ=60)

                                                                         CCATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTAGTCGTAGTCGGGCT                     >  SRR4302533.2808612/1‑94 (MQ=60)

                                                                          CATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTT                    >  SRR4302533.86917/1‑94 (MQ=60)

                                                                           ATCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTC                   >  SRR4302533.6139683/1‑94 (MQ=60)

                                                                            TCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCT                  <  SRR4302533.2571631/94‑1 (MQ=60)

                                                                            TCGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCT                  <  SRR4302533.1243272/94‑1 (MQ=60)

                                                                             CGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGGTCGCCAAACAGATTGTCGTCGTAGTTGGGCTTCTC                 >  SRR4302533.5645112/1‑94 (MQ=60)

                                                                             CGGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTC                 >  SRR4302533.482179/1‑94 (MQ=60)

                                                                              GGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCC                >  SRR4302533.4502513/1‑94 (MQ=60)

                                                                              GGCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCC                >  SRR4302533.6235408/1‑94 (MQ=60)

                                                                               GCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCT               <  SRR4302533.6120522/94‑1 (MQ=60)

                                                                               GCGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCT               <  SRR4302533.5543151/94‑1 (MQ=60)

                                                                                CGGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTT              >  SRR4302533.2926684/1‑94 (MQ=60)

                                                                                 GGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTA             <  SRR4302533.3498185/94‑1 (MQ=60)

                                                                                 GGATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTAGGGCTTCTCCTTA             <  SRR4302533.548106/94‑1 (MQ=60)

                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG            <  SRR4302533.639844/94‑1 (MQ=60)

                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG            <  SRR4302533.1089670/94‑1 (MQ=60)

                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG            >  SRR4302533.1361819/1‑94 (MQ=60)

                                                                                  GATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAG            >  SRR4302533.5266596/1‑94 (MQ=60)

                                                                                   ATTCCACATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGA           <  SRR4302533.2502267/94‑1 (MQ=60)

                                                                                         CATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT     <  SRR4302533.3736913/94‑1 (MQ=60)

                                                                                         CATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTT     >  SRR4302533.5565493/1‑94 (MQ=60)

                                                                                          ATCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTA    >  SRR4302533.3512337/1‑94 (MQ=60)

                                                                                           TCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAA   <  SRR4302533.6229312/94‑1 (MQ=60)

                                                                                           TCGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAA   >  SRR4302533.5776590/1‑94 (MQ=60)

                                                                                            CGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAG  <  SRR4302533.5827767/94‑1 (MQ=60)

                                                                                            CGGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAG  >  SRR4302533.5198137/1‑94 (MQ=60)

CGGACGCCAGACTACGCGGTCGCCGGTTACCAGCGAACGGATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAG  >  NC_000913/4388839‑4389024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`