Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,292	T→A	S101R (AGT→AGA)	mutL →	MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,292	0	T	A	100.0%	808.8 / NA	222	S101R (AGT→AGA)	mutL	MutL
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (112/110); total (112/110)

Only 100 of 242 total aligned reads displayed.

CTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395203‑4395381

ctggcTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATc                                                                                        >  1:5329437/1‑93 (MQ=255)

 tgccTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTTGCGGTGAGGCGCTGGGGAGGATCa                                                                                       >  2:3960500/4‑93 (MQ=255)

    cTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGtt                                                                                    <  1:1725373/93‑1 (MQ=255)

    cTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGtt                                                                                    <  1:5828106/93‑1 (MQ=255)

    cTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGtt                                                                                    >  1:3178114/1‑93 (MQ=255)

     tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   >  1:3032179/1‑93 (MQ=255)

     tCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTc                                                                                   >  2:4925772/1‑93 (MQ=255)

      cGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCg                                                                                  >  1:4921501/1‑93 (MQ=255)

        tCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGt                                                                                <  2:4864283/93‑1 (MQ=255)

         cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGtt                                                                               >  2:5753506/1‑93 (MQ=255)

          aTGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGttt                                                                              >  2:5251413/1‑93 (MQ=255)

           tGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTc                                                                             >  1:5187073/1‑93 (MQ=255)

           tGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTc                                                                             >  1:3449797/1‑93 (MQ=255)

            gCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTcc                                                                            <  1:5144795/93‑1 (MQ=255)

             ccaccaGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTccc                                                                           >  1:5514189/1‑93 (MQ=255)

                  aGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTg                                                                      >  1:1953030/1‑93 (MQ=255)

                   gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGTGGAGCATCAGCTCGGTTTCCCGCCTgc                                                                     >  1:4696909/1‑92 (MQ=255)

                   gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGa                                                                     <  2:1654029/93‑1 (MQ=255)

                   gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGa                                                                     >  1:1686389/1‑93 (MQ=255)

                   gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGa                                                                     <  1:5755014/93‑1 (MQ=255)

                    tAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAc                                                                    >  2:1952410/1‑93 (MQ=255)

                      aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGc                                                                  >  1:4940080/1‑93 (MQ=255)

                      aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGc                                                                  <  2:655154/93‑1 (MQ=255)

                      aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGc                                                                  >  1:4057575/1‑93 (MQ=255)

                        aTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCGTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTc                                                                >  1:4430638/1‑93 (MQ=255)

                         tCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCa                                                               <  2:351788/93‑1 (MQ=255)

                         tCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCa                                                               >  1:1398427/1‑93 (MQ=255)

                         tCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTACCGCCTGACGCTCa                                                               <  2:6269269/93‑1 (MQ=255)

                         tCGCCTCTCTGGACGATCTCGAAGCAATTATCAGCCTGGGCTTTCGCGGTGAGGGGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCa                                                               >  2:5296074/1‑93 (MQ=255)

                           gCCTCTCTGGACGATCTCGAAGACATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGCCGCTCACt                                                             >  2:6059620/1‑93 (MQ=255)

                             ctctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTc                                                           <  2:6085758/93‑1 (MQ=255)

                             ctctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTc                                                           <  2:3869656/93‑1 (MQ=255)

                             ctctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTc                                                           >  1:2140953/1‑93 (MQ=255)

                             ctctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTc                                                           <  2:1062438/93‑1 (MQ=255)

                               ctctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCAc                                                         >  1:2811136/1‑93 (MQ=255)

                                tctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACg                                                        >  1:3711852/1‑93 (MQ=255)

                                tctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACg                                                        >  2:2910052/1‑93 (MQ=255)

                                tctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACg                                                        >  2:1444508/1‑93 (MQ=255)

                                 ctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGc                                                       >  2:5694872/1‑93 (MQ=255)

                                 ctGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGc                                                       >  1:3751763/1‑93 (MQ=255)

                                  tGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCa                                                      >  2:2140647/1‑93 (MQ=255)

                                  tGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCa                                                      >  2:3152312/1‑93 (MQ=255)

                                   ggACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAc                                                     <  2:1759115/93‑1 (MQ=255)

                                    gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCCCGCAcc                                                    >  2:3758761/1‑93 (MQ=255)

                                    gACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAcc                                                    <  1:4394170/93‑1 (MQ=255)

                                     aCGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCg                                                   >  1:714092/1‑93 (MQ=255)

                                     aCGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCg                                                   >  2:437484/1‑93 (MQ=255)

                                      cGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGc                                                  >  1:3482299/1‑93 (MQ=255)

                                         tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               >  2:2513369/1‑93 (MQ=255)

                                         tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               <  1:57635/93‑1 (MQ=255)

                                         tctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGa                                               >  1:885284/1‑93 (MQ=255)

                                           tcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAc                                             >  1:1663818/1‑93 (MQ=255)

                                            cGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAca                                            <  2:4560410/93‑1 (MQ=255)

                                             gAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcag                                           >  2:2128523/1‑93 (MQ=255)

                                              aaGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagc                                          <  2:1588050/93‑1 (MQ=255)

                                               aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagca                                         >  1:1628322/1‑93 (MQ=255)

                                               aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagca                                         <  1:4547324/93‑1 (MQ=255)

                                                gCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAcagcag                                        <  2:4063943/93‑1 (MQ=255)

                                                 ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAgg                                       >  1:2728213/1‑93 (MQ=255)

                                                   aTTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGaa                                     >  1:2244251/1‑93 (MQ=255)

                                                    ttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAg                                    <  1:610743/93‑1 (MQ=255)

                                                     tATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGc                                   <  2:1169123/93‑1 (MQ=255)

                                                      aTCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGcc                                  <  2:414244/93‑1 (MQ=255)

                                                       tCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 <  1:4761919/93‑1 (MQ=255)

                                                       tCAGCCTGGGCTGTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCt                                 <  2:3329899/93‑1 (MQ=255)

                                                        cAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTg                                <  1:6206504/93‑1 (MQ=255)

                                                        cAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTg                                <  2:1360804/93‑1 (MQ=255)

                                                          gCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGc                              >  2:3184523/1‑93 (MQ=255)

                                                           ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  2:1867309/93‑1 (MQ=255)

                                                           ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  1:2705347/93‑1 (MQ=255)

                                                           ccTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCa                             <  1:816279/93‑1 (MQ=255)

                                                            cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg                            >  2:5890780/1‑93 (MQ=255)

                                                            cTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAg                            >  1:219597/1‑93 (MQ=255)

                                                             tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGCCGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           >  1:2035935/1‑93 (MQ=255)

                                                             tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           <  1:1020346/93‑1 (MQ=255)

                                                             tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           >  2:2082457/1‑93 (MQ=255)

                                                             tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAga                           >  1:1268773/1‑92 (MQ=255)

                                                             tGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCATGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAgg                           >  1:2375860/1‑93 (MQ=255)

                                                                 cTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTa                       <  2:4541686/93‑1 (MQ=255)

                                                                  tttCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTAt                      <  2:3449797/93‑1 (MQ=255)

                                                                  tttCGCGGTGAGGCGCTGGCGAGAATCAGTGCGGTTCCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTAt                      <  2:2563004/93‑1 (MQ=255)

                                                                   ttCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATg                     >  1:1854058/1‑93 (MQ=255)

                                                                    tCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGc                    <  2:2915846/93‑1 (MQ=255)

                                                                    tCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGc                    <  1:5409419/93‑1 (MQ=255)

                                                                     cgcgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTTACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGcc                   >  2:2708968/1‑93 (MQ=255)

                                                                      gcgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCg                  <  1:1163204/93‑1 (MQ=255)

                                                                      gcgGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCg                  <  2:4322788/93‑1 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                <  2:4696909/93‑1 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                >  2:3227160/1‑93 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                <  1:3620334/93‑1 (MQ=255)

                                                                        ggTGAGGCGCTGGCGAGAATCAGTTCGGTTACCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGaa                <  2:222770/93‑1 (MQ=255)

                                                                          tGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAgg              <  2:4968896/93‑1 (MQ=255)

                                                                            aGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGgc            <  2:3501610/93‑1 (MQ=255)

                                                                                 cTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGat       >  1:4165066/1‑93 (MQ=255)

                                                                                 cTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGat       >  1:230685/1‑93 (MQ=255)

                                                                                  tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata      >  2:5145275/1‑93 (MQ=255)

                                                                                  tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata      <  2:2049304/93‑1 (MQ=255)

                                                                                  tGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGata      >  2:1057539/1‑93 (MQ=255)

                                                                                    gCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATg    <  1:5991641/93‑1 (MQ=255)

                                                                                      gagaATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAACCTGGCAGGCCTATGCCGAAGGGCGCGATATGaa  >  1:1771244/1‑93 (MQ=255)

CTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA  >  NC_000913/4395203‑4395381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  >  NC_000913/4395199‑4395384

GGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGA                                                                                              <  SRR4302533.14563/94‑1 (MQ=60)

 GCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAA                                                                                             >  SRR4302533.5670595/1‑94 (MQ=60)

   GCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATC                                                                                           >  SRR4302533.5361379/1‑94 (MQ=60)

    CTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCA                                                                                          >  SRR4302533.5220262/1‑94 (MQ=60)

     TGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAG                                                                                         >  SRR4302533.5797430/1‑94 (MQ=60)

       GCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTT                                                                                       <  SRR4302533.4013206/94‑1 (MQ=60)

       GCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTT                                                                                       >  SRR4302533.3197662/1‑94 (MQ=60)

        CTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTC                                                                                      <  SRR4302533.5863323/94‑1 (MQ=60)

        CTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTC                                                                                      >  SRR4302533.3050730/1‑94 (MQ=60)

        CTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTC                                                                                      <  SRR4302533.1735843/94‑1 (MQ=60)

         TCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCG                                                                                     >  SRR4302533.4951313/1‑94 (MQ=60)

          CGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGG                                                                                    <  SRR4302533.774681/94‑1 (MQ=60)

          CGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGG                                                                                    >  SRR4302533.1841205/1‑94 (MQ=60)

           GTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGT                                                                                   <  SRR4302533.1664539/94‑1 (MQ=60)

            TCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTT                                                                                  <  SRR4302533.2741925/94‑1 (MQ=60)

             CATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTT                                                                                 <  SRR4302533.959767/94‑1 (MQ=60)

              ATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTC                                                                                >  SRR4302533.3471097/1‑94 (MQ=60)

              ATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTC                                                                                >  SRR4302533.5218382/1‑94 (MQ=60)

              ATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTC                                                                                >  SRR4302533.4252789/1‑94 (MQ=60)

               TGCCGCCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCC                                                                               <  SRR4302533.1687012/94‑1 (MQ=60)

                GCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCC                                                                              >  SRR4302533.5547478/1‑94 (MQ=60)

                GCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCC                                                                              <  SRR4302533.5175886/94‑1 (MQ=60)

                 CCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCG                                                                             >  SRR4302533.3051833/1‑94 (MQ=60)

                  CACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGC                                                                            <  SRR4302533.1772647/94‑1 (MQ=60)

                     CAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTG                                                                         >  SRR4302533.3582802/1‑94 (MQ=60)

                     CAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTG                                                                         >  SRR4302533.1964933/1‑94 (MQ=60)

                     CAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTG                                                                         <  SRR4302533.2208117/94‑1 (MQ=60)

                      AGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGTGGAGCATCAGCTCGGTTTCCCGCCTGC                                                                        >  SRR4302533.4725383/1‑94 (MQ=60)

                      AGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGA                                                                        >  SRR4302533.1696603/1‑94 (MQ=60)

                       GTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGAC                                                                       <  SRR4302533.5789739/94‑1 (MQ=60)

                         AAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGC                                                                     >  SRR4302533.4969987/1‑94 (MQ=60)

                         AAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGC                                                                     >  SRR4302533.4082275/1‑94 (MQ=60)

                           AATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCGTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTC                                                                   >  SRR4302533.4457423/1‑94 (MQ=60)

                            ATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCA                                                                  >  SRR4302533.1406855/1‑94 (MQ=60)

                              CGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACT                                                                >  SRR4302533.5925108/1‑94 (MQ=60)

                                CCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTC                                                              >  SRR4302533.2154148/1‑94 (MQ=60)

                                CCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTC                                                              <  SRR4302533.4997930/94‑1 (MQ=60)

                                  TCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCAC                                                            >  SRR4302533.2828436/1‑94 (MQ=60)

                                  TCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCAC                                                            <  SRR4302533.434105/94‑1 (MQ=60)

                                  TCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCAC                                                            <  SRR4302533.4605082/94‑1 (MQ=60)

                                   CTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACG                                                           >  SRR4302533.3734647/1‑94 (MQ=60)

                                    TCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGC                                                          >  SRR4302533.3774766/1‑94 (MQ=60)

                                     CTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCA                                                         >  SRR4302533.2365095/1‑94 (MQ=60)

                                     CTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCA                                                         >  SRR4302533.5179212/1‑94 (MQ=60)

                                     CTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCA                                                         >  SRR4302533.1338776/1‑94 (MQ=60)

                                     CTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCA                                                         >  SRR4302533.2018913/1‑94 (MQ=60)

                                      TGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCAC                                                        <  SRR4302533.603004/94‑1 (MQ=60)

                                        GACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCG                                                      <  SRR4302533.4420699/94‑1 (MQ=60)

                                        GACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCG                                                      >  SRR4302533.718308/1‑94 (MQ=60)

                                         ACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGC                                                     >  SRR4302533.3503780/1‑94 (MQ=60)

                                            ATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGA                                                  <  SRR4302533.925415/94‑1 (MQ=60)

                                            ATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGA                                                  >  SRR4302533.890485/1‑94 (MQ=60)

                                             TCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAA                                                 <  SRR4302533.58017/94‑1 (MQ=60)

                                              CTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAAC                                                >  SRR4302533.1673876/1‑94 (MQ=60)

                                                  AAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCA                                            >  SRR4302533.1638182/1‑94 (MQ=60)

                                                  AAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCA                                            >  SRR4302533.6203187/1‑94 (MQ=60)

                                                   AGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAG                                           <  SRR4302533.4953878/94‑1 (MQ=60)

                                                   AGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAG                                           <  SRR4302533.4574862/94‑1 (MQ=60)

                                                    GCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGG                                          <  SRR4302533.5451683/94‑1 (MQ=60)

                                                    GCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGG                                          <  SRR4302533.4822809/94‑1 (MQ=60)

                                                    GCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGG                                          >  SRR4302533.2744949/1‑94 (MQ=60)

                                                     CCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGA                                         >  SRR4302533.3370704/1‑94 (MQ=60)

                                                     CCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGA                                         >  SRR4302533.4484423/1‑94 (MQ=60)

                                                     CCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGA                                         <  SRR4302533.5630320/94‑1 (MQ=60)

                                                      CATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAA                                        <  SRR4302533.3682814/94‑1 (MQ=60)

                                                      CATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAA                                        >  SRR4302533.2258121/1‑94 (MQ=60)

                                                       ATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAG                                       >  SRR4302533.4507157/1‑94 (MQ=60)

                                                       ATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAG                                       <  SRR4302533.3898253/94‑1 (MQ=60)

                                                       ATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAG                                       >  SRR4302533.749424/1‑94 (MQ=60)

                                                        TTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCGCCCGCACGCTCACTTCACCCACCCCAGAACAGCA‑GAAGCC                                     >  SRR4302533.5815179/1‑94 (MQ=60)

                                                        TTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGC                                      <  SRR4302533.614332/94‑1 (MQ=60)

                                                         TATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGTACCGCAGAACAGCAGGAAGCC                                     <  SRR4302533.4431573/94‑1 (MQ=60)

                                                         TATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCC                                     >  SRR4302533.4937673/1‑94 (MQ=60)

                                                          ATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCT                                    <  SRR4302533.118896/94‑1 (MQ=60)

                                                          ATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCT                                    >  SRR4302533.1580914/1‑94 (MQ=60)

                                                           TCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTG                                   <  SRR4302533.4790804/94‑1 (MQ=60)

                                                           TCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTG                                   <  SRR4302533.5838479/94‑1 (MQ=60)

                                                            CAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGG                                  <  SRR4302533.6243724/94‑1 (MQ=60)

                                                            CAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGG                                  <  SRR4302533.1058210/94‑1 (MQ=60)

                                                             atctTGGGCTGTCGCGGTGAGGCGCTGGGGAGACTCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGC                                 <  SRR4302533.2857976/90‑1 (MQ=60)

                                                              GCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCATCGCAGAACAGCAGGAAGCCTGGCA                                >  SRR4302533.5364949/1‑94 (MQ=60)

                                                              GCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCA                                >  SRR4302533.2561420/1‑94 (MQ=60)

                                                              GCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCA                                <  SRR4302533.17071/94‑1 (MQ=60)

                                                              GCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCA                                <  SRR4302533.187599/94‑1 (MQ=60)

                                                               CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               <  SRR4302533.821063/94‑1 (MQ=60)

                                                               CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               >  SRR4302533.3564985/1‑94 (MQ=60)

                                                               CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               <  SRR4302533.2721940/94‑1 (MQ=60)

                                                               CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               >  SRR4302533.2248790/1‑94 (MQ=60)

                                                               CCTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAG                               >  SRR4302533.220971/1‑94 (MQ=60)

                                                                CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGCCGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGG                              >  SRR4302533.2048490/1‑94 (MQ=60)

                                                                CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGG                              >  SRR4302533.6227945/1‑94 (MQ=60)

                                                                CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGG                              >  SRR4302533.5641153/1‑94 (MQ=60)

                                                                CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGG                              >  SRR4302533.6300604/1‑94 (MQ=60)

                                                                CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGA                              >  SRR4302533.1276368/1‑94 (MQ=60)

                                                                CTGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCATGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGG                              >  SRR4302533.2390560/1‑94 (MQ=60)

                                                                 TGGGCTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGC                             <  SRR4302533.1026340/94‑1 (MQ=60)

                                                                     CTTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTAT                         >  SRR4302533.1897508/1‑94 (MQ=60)

                                                                      TTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATG                        >  SRR4302533.1865362/1‑94 (MQ=60)

                                                                      TTTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATG                        >  SRR4302533.2062945/1‑94 (MQ=60)

                                                                       TTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGC                       >  SRR4302533.1292243/1‑94 (MQ=60)

                                                                       TTCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGC                       <  SRR4302533.2994353/94‑1 (MQ=60)

                                                                        TCGCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCC                      <  SRR4302533.5441933/94‑1 (MQ=60)

                                                                          GCGGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGA                    <  SRR4302533.1170182/94‑1 (MQ=60)

                                                                            GGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  <  SRR4302533.2639176/94‑1 (MQ=60)

                                                                            GGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  <  SRR4302533.3642608/94‑1 (MQ=60)

                                                                            GGTGAGGCGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAG                  <  SRR4302533.2153841/94‑1 (MQ=60)

                                                                                   CGCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGA           >  SRR4302533.2266385/1‑94 (MQ=60)

                                                                                    GCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGAT          >  SRR4302533.4190302/1‑94 (MQ=60)

                                                                                    GCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGAT          <  SRR4302533.855635/94‑1 (MQ=60)

                                                                                    GCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGAT          >  SRR4302533.232114/1‑94 (MQ=60)

                                                                                    GCTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGAT          >  SRR4302533.2804376/1‑94 (MQ=60)

                                                                                     CTGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATA         >  SRR4302533.1640385/1‑94 (MQ=60)

                                                                                      TGGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATAT        <  SRR4302533.5019123/94‑1 (MQ=60)

                                                                                      TGGCGAGAATCAGTTCGGTTTCCCGCATGACGCGCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATAT        <  SRR4302533.230166/94‑1 (MQ=60)

                                                                                       GGCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATG       <  SRR4302533.5403349/94‑1 (MQ=60)

                                                                                        GCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGA      <  SRR4302533.6027934/94‑1 (MQ=60)

                                                                                        GCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGA      <  SRR4302533.4143889/94‑1 (MQ=60)

                                                                                        GCGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGA      >  SRR4302533.3490770/1‑94 (MQ=60)

                                                                                         CGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA     >  SRR4302533.1767229/1‑94 (MQ=60)

                                                                                         CGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA     <  SRR4302533.4772852/94‑1 (MQ=60)

                                                                                         CGAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAACCTGGCAGGCCTATGCCGAAGGGCGCGATATGAA     >  SRR4302533.1782026/1‑94 (MQ=60)

                                                                                          GAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAAC    <  SRR4302533.3740962/94‑1 (MQ=60)

                                                                                          GAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAAC    >  SRR4302533.924716/1‑94 (MQ=60)

                                                                                          GAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAAC    <  SRR4302533.3397102/94‑1 (MQ=60)

                                                                                          GAGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGACTATGCCGAAGGGCGCGATATGAAC    >  SRR4302533.5811285/1‑94 (MQ=60)

                                                                                           AGAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGACTATGCCGAAGGGCGCGATATGAACG   >  SRR4302533.1087085/1‑94 (MQ=60)

                                                                                            GAATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  <  SRR4302533.730230/94‑1 (MQ=60)

GGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCACGCACCGCAGAACAGCAGGAAGCCTGGCAGGCCTATGCCGAAGGGCGCGATATGAACGT  >  NC_000913/4395199‑4395384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`