Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,406,298	A→G	Q689Q (CAA→CAG)	b4179 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,406,298	0	A	G	99.7%	1136.0 / ‑5.1	352	Q689Q (CAA→CAG)	b4179	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (196/155); total (197/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 366 total aligned reads displayed.

CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAA  >  NC_000913/4406208‑4406387

cttcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCg                                                                                         <  1:2522885/93‑1 (MQ=255)

 ttcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAgcgc                                                                                        >  1:7865353/1‑93 (MQ=255)

 ttcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAgcgc                                                                                        >  1:7764275/1‑93 (MQ=255)

     tccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGa                                                                                    <  2:1456946/93‑1 (MQ=255)

     tccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGCGCAGCGCCTGa                                                                                    >  1:157744/1‑93 (MQ=255)

     tccgtcTAGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGa                                                                                    <  2:100186/93‑1 (MQ=255)

      ccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGAt                                                                                   <  1:5466605/93‑1 (MQ=255)

      ccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGAt                                                                                   <  2:6970447/93‑1 (MQ=255)

       cgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATg                                                                                  <  2:5813316/93‑1 (MQ=255)

       cgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATg                                                                                  <  1:3523358/93‑1 (MQ=255)

       cgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATg                                                                                  <  2:7398215/93‑1 (MQ=255)

        gtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATgg                                                                                 >  2:9293069/1‑93 (MQ=255)

        gtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATgg                                                                                 >  2:1034705/1‑93 (MQ=255)

        gtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATgg                                                                                 >  2:6500910/1‑93 (MQ=255)

         tcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATggg                                                                                <  1:1646084/93‑1 (MQ=255)

           tGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATggggg                                                                              >  1:6539761/1‑93 (MQ=255)

           tGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATggggg                                                                              <  2:4503511/93‑1 (MQ=255)

            ggACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATgggggt                                                                             >  1:3748071/1‑92 (MQ=255)

                gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATcc                                                                         <  2:6150317/93‑1 (MQ=255)

                gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATcc                                                                         <  1:8016635/93‑1 (MQ=255)

                gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATcc                                                                         >  2:4785000/1‑93 (MQ=255)

                  cTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAg                                                                       >  1:7087580/1‑93 (MQ=255)

                   ttGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGc                                                                      <  2:2346747/93‑1 (MQ=255)

                           tGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGAc                                                              >  1:1377995/1‑93 (MQ=255)

                           tGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGAc                                                              >  2:1808981/1‑93 (MQ=255)

                            gATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGAGCCTGATGGGGGAATCCAGCGGCCAGACt                                                             >  1:871576/1‑93 (MQ=255)

                              tggtctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTa                                                           <  2:6352220/93‑1 (MQ=255)

                               ggtctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTAt                                                          >  1:5832481/1‑93 (MQ=255)

                                gtctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATc                                                         <  2:4069697/93‑1 (MQ=255)

                                gtctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATc                                                         >  1:7782924/1‑93 (MQ=255)

                                 tctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATAGGGGAATCCAGCGGCCAGACTTATCg                                                        >  1:9687088/1‑93 (MQ=255)

                                   tggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGcc                                                      >  2:7917406/1‑93 (MQ=255)

                                    ggtcCATGTCTCTTCGCTGGACAATGACTACTAGCGCTTGGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCt                                                     <  1:4258662/93‑1 (MQ=255)

                                     gtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTg                                                    <  2:8860294/93‑1 (MQ=255)

                                      tcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTgg                                                   <  1:9379971/93‑1 (MQ=255)

                                         aTGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCg                                                >  1:5694115/1‑93 (MQ=255)

                                          tGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGa                                               >  1:271990/1‑93 (MQ=255)

                                          tGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGa                                               >  2:6099310/1‑93 (MQ=255)

                                           gTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGAt                                              <  2:9111883/93‑1 (MQ=255)

                                           gTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGAt                                              <  1:1002057/93‑1 (MQ=255)

                                               ctTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATcgcg                                          >  2:7201437/1‑93 (MQ=255)

                                               ctTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATcgcg                                          >  2:3921024/1‑93 (MQ=255)

                                                ttCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGt                                         >  1:1132074/1‑93 (MQ=255)

                                                ttCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGt                                         >  1:7280451/1‑93 (MQ=255)

                                                ttCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGt                                         >  1:6337735/1‑93 (MQ=255)

                                                   gCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGa                                      <  2:5914429/93‑1 (MQ=255)

                                                   gCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGa                                      >  2:3326392/1‑93 (MQ=255)

                                                    cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGag                                     >  1:3048951/1‑92 (MQ=255)

                                                    cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGaa                                     <  1:6109216/93‑1 (MQ=255)

                                                    cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGaa                                     <  2:2090477/93‑1 (MQ=255)

                                                      ggACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGt                                   >  1:5516836/1‑93 (MQ=255)

                                                       gACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGtt                                  <  1:5412793/93‑1 (MQ=255)

                                                       gACAATGACTACTATCACTTTGACCAGGTAGGGCAGCGCCTGGTGGGGGAATCCAGCGGCCAGACTTATCGCCTGGACGATCGAATGGGAGta                                  <  2:8986719/93‑2 (MQ=255)

                                                        aCAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTc                                 <  2:1645534/93‑1 (MQ=255)

                                                          aaTGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTcgc                               >  2:8491865/1‑93 (MQ=255)

                                                          aaTGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTcgc                               <  1:2764227/93‑1 (MQ=255)

                                                           aTGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTcgcg                              >  1:1277421/1‑93 (MQ=255)

                                                            tGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGt                             <  2:7666288/93‑1 (MQ=255)

                                                             gACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTc                            <  2:9566283/93‑1 (MQ=255)

                                                              actactATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCg                           >  2:6758340/1‑93 (MQ=255)

                                                              actactATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCg                           >  1:380805/1‑93 (MQ=255)

                                                               ctactaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGa                          >  2:5007153/1‑93 (MQ=255)

                                                               ctactaGCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGa                          <  2:511472/93‑1 (MQ=255)

                                                                tactaTCTCTTTGACCAGGGAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGaa                         <  2:8129839/93‑1 (MQ=255)

                                                                tactaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGaa                         >  2:1859518/1‑93 (MQ=255)

                                                                tactaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGaa                         >  2:1290267/1‑93 (MQ=255)

                                                                 actaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAg                        >  1:2137441/1‑93 (MQ=255)

                                                                  ctaTCGCTTTGACCAGGTATGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGc                       >  1:6977815/1‑93 (MQ=255)

                                                                  ctaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGc                       <  1:5996320/93‑1 (MQ=255)

                                                                   taTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGCAGCg                      >  1:4964420/1‑93 (MQ=255)

                                                                   taTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCg                      >  2:4639112/1‑93 (MQ=255)

                                                                   taTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCg                      >  2:2749139/1‑93 (MQ=255)

                                                                   taTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGCTCGCGTCGAAGCg                      >  2:687312/1‑93 (MQ=255)

                                                                    aTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCgg                     >  1:4741041/1‑93 (MQ=255)

                                                                        cTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTat                 >  1:1238655/1‑92 (MQ=255)

                                                                        cTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTaa                 >  1:7681860/1‑93 (MQ=255)

                                                                        cTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTaa                 >  2:4167593/1‑93 (MQ=255)

                                                                        cTTTGACCAGGTAGGGCAGCGCCTGATGGGAGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTaa                 <  2:6660906/93‑1 (MQ=255)

                                                                         tttGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAat                <  2:3689556/93‑1 (MQ=255)

                                                                          ttGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAata               <  2:2258413/93‑1 (MQ=255)

                                                                          ttGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAata               <  1:4182909/93‑1 (MQ=255)

                                                                           tGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAatat              <  1:8181320/93‑1 (MQ=255)

                                                                           tGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAatat              <  1:9472339/93‑1 (MQ=255)

                                                                           tGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAatat              <  2:345311/93‑1 (MQ=255)

                                                                           tGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAatat              <  2:9352227/93‑1 (MQ=255)

                                                                           tGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGGTCGCGTCGAAGCGGTTAatat              >  1:1941965/1‑93 (MQ=255)

                                                                              ccAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGa           <  1:75097/93‑1 (MQ=255)

                                                                              acAGGTAGGGCAGCGCCTGATGGCGGAATCCAGCGGCCAGACTTATCGCCTGTGGGACCGCGTGGAAGTTCGCTGCGAAGCGGTTAATATCGa           <  2:5509322/92‑1 (MQ=255)

                                                                               cAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGAc          <  1:6706155/93‑1 (MQ=255)

                                                                               cAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGAc          >  1:9197100/1‑93 (MQ=255)

                                                                                 ggTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGTGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa        >  2:3552993/1‑93 (MQ=255)

                                                                                 ggTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa        >  2:3172805/1‑93 (MQ=255)

                                                                                 ggTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa        <  1:3241928/93‑1 (MQ=255)

                                                                                  gTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAg       >  1:5111133/1‑93 (MQ=255)

                                                                                  gTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAg       >  2:2637162/1‑93 (MQ=255)

                                                                                   tAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgc      >  1:23610/1‑93 (MQ=255)

                                                                                   tAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgc      <  1:4378751/93‑1 (MQ=255)

                                                                                    aGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgcg     >  2:536273/1‑93 (MQ=255)

                                                                                     gggCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgcgc    >  2:827735/1‑93 (MQ=255)

                                                                                      ggCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCa   >  1:2216986/1‑93 (MQ=255)

CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAA  >  NC_000913/4406208‑4406387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATC  >  NC_000913/4406205‑4406391

CTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAG                                                                                               >  SRR4302540.4964181/1‑94 (MQ=60)

CTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAG                                                                                               <  SRR4302540.9185536/94‑1 (MQ=60)

 TTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGC                                                                                              >  SRR4302540.9736512/1‑94 (MQ=60)

 TTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGC                                                                                              >  SRR4302540.9562999/1‑94 (MQ=60)

 TTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGC                                                                                              >  SRR4302540.2920957/1‑94 (MQ=60)

 TTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGC                                                                                              >  SRR4302540.7650837/1‑94 (MQ=60)

 TTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGC                                                                                              >  SRR4302540.7650838/1‑94 (MQ=60)

 TTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGC                                                                                              >  SRR4302540.902892/1‑94 (MQ=60)

  TCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCG                                                                                             >  SRR4302540.2759680/1‑94 (MQ=60)

   CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGC                                                                                            >  SRR4302540.1557151/1‑94 (MQ=60)

   CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGC                                                                                            <  SRR4302540.2550120/94‑1 (MQ=60)

   CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGC                                                                                            >  SRR4302540.5481456/1‑94 (MQ=60)

   CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGC                                                                                            >  SRR4302540.7855037/1‑94 (MQ=60)

   CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGC                                                                                            >  SRR4302540.7957243/1‑94 (MQ=60)

   CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGAACAGGTAGGGCAGCGC                                                                                            >  SRR4302540.7251326/1‑94 (MQ=60)

    TTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCC                                                                                           <  SRR4302540.7430419/94‑1 (MQ=60)

      CGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTG                                                                                         <  SRR4302540.5612481/94‑1 (MQ=60)

       GTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGCGCAGCGCCTGA                                                                                        >  SRR4302540.159340/1‑94 (MQ=60)

        TCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGAT                                                                                       <  SRR4302540.2697688/94‑1 (MQ=60)

         CCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATG                                                                                      <  SRR4302540.5526779/94‑1 (MQ=60)

         CCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATG                                                                                      >  SRR4302540.403206/1‑94 (MQ=60)

          CGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCCGCGCCTGATGG                                                                                     <  SRR4302540.9065326/94‑1 (MQ=60)

          CGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGG                                                                                     <  SRR4302540.3561773/94‑1 (MQ=60)

           GTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGG                                                                                    >  SRR4302540.6131687/1‑94 (MQ=60)

           GTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGG                                                                                    <  SRR4302540.5536201/94‑1 (MQ=60)

            TCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGG                                                                                   <  SRR4302540.1664053/94‑1 (MQ=60)

             CTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGG                                                                                  >  SRR4302540.6612334/1‑94 (MQ=60)

              TGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGT                                                                                 >  SRR4302540.3788968/1‑94 (MQ=60)

              TGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGA                                                                                 >  SRR4302540.2801048/1‑94 (MQ=60)

               GGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAA                                                                                >  SRR4302540.4243208/1‑94 (MQ=60)

                GACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAAT                                                                               <  SRR4302540.1171551/94‑1 (MQ=60)

                  CGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCC                                                                             >  SRR4302540.6643134/1‑94 (MQ=60)

                  CGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCA                                                                             >  SRR4302540.5406187/1‑94 (MQ=60)

                   GACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCA                                                                            <  SRR4302540.8109878/94‑1 (MQ=60)

                    ACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAG                                                                           >  SRR4302540.7166452/1‑94 (MQ=60)

                      TTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGTG                                                                         >  SRR4302540.8759494/1‑94 (MQ=60)

                      TTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCG                                                                         >  SRR4302540.5731209/1‑94 (MQ=60)

                      TTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCG                                                                         <  SRR4302540.2057043/94‑1 (MQ=60)

                      TTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCG                                                                         <  SRR4302540.5243290/94‑1 (MQ=60)

                        GTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGC                                                                       >  SRR4302540.5137258/1‑94 (MQ=60)

                        GTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGC                                                                       >  SRR4302540.7828995/1‑94 (MQ=60)

                         TTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTATGGCAGCGCCTGATGGGGGAATCCAGCGGCC                                                                      >  SRR4302540.5480925/1‑94 (MQ=60)

                         TTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCC                                                                      >  SRR4302540.8061522/1‑94 (MQ=60)

                         TTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCC                                                                      >  SRR4302540.5480926/1‑94 (MQ=60)

                         TTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCC                                                                      >  SRR4302540.3190131/1‑94 (MQ=60)

                           CATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAG                                                                    <  SRR4302540.1318793/94‑1 (MQ=60)

                           CATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAG                                                                    <  SRR4302540.1636664/94‑1 (MQ=60)

                             TTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGAC                                                                  >  SRR4302540.1392663/1‑94 (MQ=60)

                              TGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACT                                                                 <  SRR4302540.2017098/94‑1 (MQ=60)

                              TGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGAGCCTGATGGGGGAATCCAGCGGCCAGACT                                                                 >  SRR4302540.880828/1‑94 (MQ=60)

                                 TTGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTAT                                                              <  SRR4302540.1266354/94‑1 (MQ=60)

                                 TGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTAT                                                              >  SRR4302540.5896975/1‑94 (MQ=60)

                                 TGGTCGGGTTCATGTCTCTTCGCCGGACAATGACTACTATCGCTTGGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTAT                                                              <  SRR4302540.1266353/94‑1 (MQ=60)

                                  GGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATC                                                             >  SRR4302540.7873897/1‑94 (MQ=60)

                                  GGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATC                                                             <  SRR4302540.947749/94‑1 (MQ=60)

                                   GTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCG                                                            >  SRR4302540.2412310/1‑94 (MQ=60)

                                   GTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCG                                                            >  SRR4302540.6353813/1‑94 (MQ=60)

                                   GTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATAGGGGAATCCAGCGGCCAGACTTATCG                                                            >  SRR4302540.9801043/1‑94 (MQ=60)

                                      TGGTCGATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCT                                                         <  SRR4302540.6203252/94‑1 (MQ=60)

                                      TGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCT                                                         <  SRR4302540.6818769/94‑1 (MQ=60)

                                      TGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGAGCCTGATGGGGGActacagcgggcgggctactcgctg                                                         >  SRR4302540.2212817/1‑70 (MQ=60)

                                       GGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCTGCCCGACTTATCGCCTG                                                        >  SRR4302540.3792775/1‑94 (MQ=60)

                                       GGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTG                                                        >  SRR4302540.2792379/1‑94 (MQ=60)

                                       GGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTG                                                        >  SRR4302540.3793034/1‑94 (MQ=60)

                                       GGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTG                                                        <  SRR4302540.3027160/94‑1 (MQ=60)

                                       GGTCCATGTCTCTTCGCTGGACAATGACTACTAGCGCTTGGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTG                                                        <  SRR4302540.4304989/94‑1 (MQ=60)

                                        GTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGG                                                       >  SRR4302540.3363741/1‑94 (MQ=60)

                                        GTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGG                                                       <  SRR4302540.3129019/94‑1 (MQ=60)

                                         TCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGG                                                      <  SRR4302540.1662120/94‑1 (MQ=60)

                                         TCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGG                                                      <  SRR4302540.9491626/94‑1 (MQ=60)

                                          CCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGC                                                     <  SRR4302540.8787297/94‑1 (MQ=60)

                                           CATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCG                                                    >  SRR4302540.5756809/1‑94 (MQ=60)

                                            ATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGNCCAGACTTATCGCCTGGGCGA                                                   >  SRR4302540.1571023/1‑94 (MQ=60)

                                            ATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGA                                                   >  SRR4302540.274669/1‑94 (MQ=60)

                                            ATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGA                                                   >  SRR4302540.7673197/1‑94 (MQ=60)

                                             TGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGAT                                                  >  SRR4302540.7049963/1‑94 (MQ=60)

                                              GTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATC                                                 <  SRR4302540.1012236/94‑1 (MQ=60)

                                              GTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATC                                                 <  SRR4302540.2546469/94‑1 (MQ=60)

                                               TCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCG                                                <  SRR4302540.5776400/94‑1 (MQ=60)

                                               TCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCG                                                >  SRR4302540.4420201/1‑94 (MQ=60)

                                                CTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGC                                               >  SRR4302540.1771543/1‑94 (MQ=60)

                                                 TCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCG                                              <  SRR4302540.7852013/94‑1 (MQ=60)

                                                 TCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCG                                              <  SRR4302540.9711829/94‑1 (MQ=60)

                                                  CTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGT                                             >  SRR4302540.1143860/1‑94 (MQ=60)

                                                  CTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGT                                             <  SRR4302540.1134310/94‑1 (MQ=60)

                                                  CTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGT                                             >  SRR4302540.6408313/1‑94 (MQ=60)

                                                  CTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGT                                             <  SRR4302540.6598484/94‑1 (MQ=60)

                                                  CTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGT                                             >  SRR4302540.7361689/1‑94 (MQ=60)

                                                   TTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTG                                            >  SRR4302540.1045726/1‑94 (MQ=60)

                                                     CGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGA                                          <  SRR4302540.930381/94‑1 (MQ=60)

                                                     CGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGA                                          <  SRR4302540.8988275/94‑1 (MQ=60)

                                                     CGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGA                                          <  SRR4302540.6367046/94‑1 (MQ=60)

                                                      GCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGNTGGGGGAATCCAGCGGCCGGACTNNNNGCNTGGGCGAngncgtgnnn                                         >  SRR4302540.3151185/1‑84 (MQ=60)

                                                      GCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGTGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAA                                         <  SRR4302540.5816289/94‑1 (MQ=60)

                                                      GCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAG                                         >  SRR4302540.3082402/1‑94 (MQ=60)

                                                      GCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAA                                         <  SRR4302540.9588943/94‑1 (MQ=60)

                                                      GCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAA                                         <  SRR4302540.5816288/94‑1 (MQ=60)

                                                       gatctagcgcttTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        <  SRR4302540.7609706/82‑1 (MQ=60)

                                                       CTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        <  SRR4302540.6177251/94‑1 (MQ=60)

                                                       CTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        >  SRR4302540.5069985/1‑94 (MQ=60)

                                                       CTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        >  SRR4302540.9740611/1‑94 (MQ=60)

                                                       CTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        <  SRR4302540.3504027/94‑1 (MQ=60)

                                                       CTGGACAATGACGAGTATCGCTTGGGCCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        <  SRR4302540.285736/94‑1 (MQ=60)

                                                        TGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGT                                       >  SRR4302540.5577601/1‑94 (MQ=60)

                                                          GACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTC                                     <  SRR4302540.5472306/94‑1 (MQ=60)

                                                          GACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTC                                     <  SRR4302540.6176339/94‑1 (MQ=60)

                                                          GACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTC                                     <  SRR4302540.7917988/94‑1 (MQ=60)

                                                           ACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCG                                    <  SRR4302540.4220803/94‑1 (MQ=60)

                                                            CAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGG                                   >  SRR4302540.684219/1‑94 (MQ=60)

                                                            CAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGC                                   <  SRR4302540.1721292/94‑1 (MQ=60)

                                                            CAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGGTCGC                                   >  SRR4302540.1984017/1‑94 (MQ=60)

                                                            CAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGAGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGGTCGC                                   >  SRR4302540.4885216/1‑94 (MQ=60)

                                                             AATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCG                                  >  SRR4302540.1290589/1‑94 (MQ=60)

                                                             AATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCG                                  <  SRR4302540.2794094/94‑1 (MQ=60)

                                                             AATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCG                                  >  SRR4302540.1290588/1‑94 (MQ=60)

                                                              ATGACTACTATCGCTTTGACCAGGTAGGGCAGTGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGT                                 >  SRR4302540.2260024/1‑94 (MQ=60)

                                                              ATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGT                                 >  SRR4302540.9379839/1‑94 (MQ=60)

                                                               TGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCCGCGGCCAGACTTATCGCCTGGGAGATCGCGTGGAAGTTCGCGTC                                >  SRR4302540.2676398/1‑94 (MQ=60)

                                                               TGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTC                                <  SRR4302540.1923450/94‑1 (MQ=60)

                                                               TGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTC                                <  SRR4302540.7873337/94‑1 (MQ=60)

                                                                GACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCG                               >  SRR4302540.384745/1‑94 (MQ=60)

                                                                 ACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGA                              >  SRR4302540.7899791/1‑94 (MQ=60)

                                                                 ACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGGTGGCGTCGA                              >  SRR4302540.8266611/1‑94 (MQ=60)

                                                                  CTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAA                             <  SRR4302540.6739840/94‑1 (MQ=60)

                                                                  CTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAA                             >  SRR4302540.6109433/1‑94 (MQ=60)

                                                                  CTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAA                             >  SRR4302540.8105380/1‑94 (MQ=60)

                                                                   TACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAG                            >  SRR4302540.2224893/1‑94 (MQ=60)

                                                                   TACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAG                            >  SRR4302540.2160403/1‑94 (MQ=60)

                                                                    ACTATCGCTTTGACCAGGTATGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGC                           >  SRR4302540.7055341/1‑94 (MQ=60)

                                                                    ACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGG                           >  SRR4302540.7524864/1‑94 (MQ=60)

                                                                    ACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGC                           >  SRR4302540.7524863/1‑94 (MQ=60)

                                                                    ACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGC                           <  SRR4302540.2781264/94‑1 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGGGGCGAAGCG                          >  SRR4302540.365596/1‑94 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGCAGCG                          >  SRR4302540.5018114/1‑94 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCG                          >  SRR4302540.8243325/1‑94 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCG                          >  SRR4302540.9532546/1‑94 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCG                          >  SRR4302540.2447568/1‑94 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCG                          <  SRR4302540.6062767/94‑1 (MQ=60)

                                                                      TATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGG                         >  SRR4302540.4791636/1‑94 (MQ=60)

                                                                      TATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGG                         >  SRR4302540.4287236/1‑94 (MQ=60)

                                                                       ATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGT                        >  SRR4302540.4920763/1‑94 (MQ=60)

                                                                         CGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTA                      >  SRR4302540.4345074/1‑94 (MQ=60)

                                                                         CGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTA                      <  SRR4302540.4121335/94‑1 (MQ=60)

                                                                         CGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTA                      <  SRR4302540.8259157/94‑1 (MQ=60)

                                                                         CGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTA                      <  SRR4302540.7213487/94‑1 (MQ=60)

                                                                          GCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAT                     >  SRR4302540.1251302/1‑94 (MQ=60)

                                                                          GCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAA                     >  SRR4302540.7870375/1‑94 (MQ=60)

                                                                          GCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAA                     >  SRR4302540.1299635/1‑94 (MQ=60)

                                                                          GCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAA                     >  SRR4302540.7870376/1‑94 (MQ=60)

                                                                          GCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAA                     >  SRR4302540.7768855/1‑94 (MQ=60)

                                                                          GCTTGGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAA                     <  SRR4302540.642327/94‑1 (MQ=60)

                                                                           CTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAAT                    <  SRR4302540.7075260/94‑1 (MQ=60)

                                                                           CTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAAT                    >  SRR4302540.7228899/1‑94 (MQ=60)

                                                                           CTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAAT                    <  SRR4302540.3615972/94‑1 (MQ=60)

                                                                            TTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATA                   >  SRR4302540.121081/1‑94 (MQ=60)

                                                                            TTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATA                   >  SRR4302540.2349664/1‑94 (MQ=60)

                                                                            TTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATA                   <  SRR4302540.3436247/94‑1 (MQ=60)

                                                                             TTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGTGTCGAAGCGGTTAATAT                  <  SRR4302540.2254624/94‑1 (MQ=60)

                                                                             TTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATAT                  <  SRR4302540.4228357/94‑1 (MQ=60)

                                                                             TTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGGTCGCGTCGAAGCGGTTAATAT                  >  SRR4302540.1963111/1‑94 (MQ=60)

                                                                              TGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATG                 <  SRR4302540.8276349/94‑1 (MQ=60)

                                                                              TGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATG                 <  SRR4302540.9584690/94‑1 (MQ=60)

                                                                               GACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGG                <  SRR4302540.7142352/94‑1 (MQ=60)

                                                                               GACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGG                >  SRR4302540.5312734/1‑94 (MQ=60)

                                                                                ACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGA               >  SRR4302540.3067512/1‑94 (MQ=60)

                                                                                 CCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGAC              <  SRR4302540.75873/94‑1 (MQ=60)

                                                                                 CCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGAC              <  SRR4302540.1189469/94‑1 (MQ=60)

                                                                                 CCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGAC              <  SRR4302540.8010263/94‑1 (MQ=60)

                                                                                 CCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGAC              >  SRR4302540.9307425/1‑94 (MQ=60)

                                                                                 CCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGAC              <  SRR4302540.425621/94‑1 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             <  SRR4302540.9863491/94‑1 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             >  SRR4302540.9218043/1‑94 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             <  SRR4302540.1462173/94‑1 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             >  SRR4302540.4645720/1‑94 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             <  SRR4302540.6780166/94‑1 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             >  SRR4302540.2185867/1‑94 (MQ=60)

                                                                                   CGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGA            <  SRR4302540.5529805/94‑1 (MQ=60)

                                                                                    GGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAG           >  SRR4302540.5166455/1‑94 (MQ=60)

                                                                                    GGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAG           <  SRR4302540.3277214/94‑1 (MQ=60)

                                                                                     GTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGC          <  SRR4302540.9545477/94‑1 (MQ=60)

                                                                                     GTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGC          <  SRR4302540.7774083/94‑1 (MQ=60)

                                                                                     GTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGC          >  SRR4302540.23872/1‑94 (MQ=60)

                                                                                     GTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGC          >  SRR4302540.5980886/1‑94 (MQ=60)

                                                                                     GTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGC          <  SRR4302540.5903433/94‑1 (MQ=60)

                                                                                      TAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCG         <  SRR4302540.4426298/94‑1 (MQ=60)

                                                                                      TAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCG         <  SRR4302540.4906584/94‑1 (MQ=60)

                                                                                      TAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCG         <  SRR4302540.4560392/94‑1 (MQ=60)

                                                                                      TAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCG         >  SRR4302540.10111099/1‑94 (MQ=60)

                                                                                       AGGGGAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGC        <  SRR4302540.5639095/94‑1 (MQ=60)

                                                                                       AGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGC        >  SRR4302540.3752811/1‑94 (MQ=60)

                                                                                        GGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCA       >  SRR4302540.2240904/1‑94 (MQ=60)

                                                                                        GGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCA       >  SRR4302540.4729078/1‑94 (MQ=60)

                                                                                         GGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAA      >  SRR4302540.669016/1‑94 (MQ=60)

                                                                                           CAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAA    >  SRR4302540.2030692/1‑94 (MQ=60)

                                                                                            AGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAAT   >  SRR4302540.3782210/1‑94 (MQ=60)

                                                                                            AGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAAT   <  SRR4302540.1250194/94‑1 (MQ=60)

                                                                                            AGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAAT   >  SRR4302540.7604780/1‑94 (MQ=60)

                                                                                            AGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAAT   >  SRR4302540.2150226/1‑94 (MQ=60)

                                                                                             GCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATC  >  SRR4302540.1636552/1‑94 (MQ=60)

CTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATC  >  NC_000913/4406205‑4406391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`