Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,406,298	A→G	Q689Q (CAA→CAG)	b4179 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,406,298	0	A	G	100.0%	812.4 / NA	251	Q689Q (CAA→CAG)	b4179	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (154/97); total (154/97)

Only 100 of 253 total aligned reads displayed.

CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAA  >  NC_000913/4406208‑4406387

 ttcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAgcgc                                                                                        >  1:2139979/1‑93 (MQ=255)

  tcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGcc                                                                                       >  2:635472/1‑93 (MQ=255)

  tcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGcc                                                                                       <  2:5157348/93‑1 (MQ=255)

    gtccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTg                                                                                     >  1:961729/1‑93 (MQ=255)

     tccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGa                                                                                    >  1:6246239/1‑93 (MQ=255)

      ccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGAt                                                                                   <  1:5858774/93‑1 (MQ=255)

       cgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATg                                                                                  >  2:4257927/1‑93 (MQ=255)

        ggcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTGTCTTCGCTGGACATTGACTACTATCTCTTTGACTAGGGAGGGCAGCGCCTTATgg                                                                                 >  1:6306187/3‑93 (MQ=255)

         tcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATggg                                                                                >  2:5198655/1‑93 (MQ=255)

         tcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATggg                                                                                <  2:1597350/93‑1 (MQ=255)

         tcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATggg                                                                                <  1:2705984/93‑1 (MQ=255)

           tGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCCCTTTGAGCAGGTAGGGCAGCGACTGATggggg                                                                              >  2:938463/1‑93 (MQ=255)

            ggACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGa                                                                             >  2:1237546/1‑93 (MQ=255)

             gacgacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGaa                                                                            >  1:3551389/1‑93 (MQ=255)

             gacgacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGaa                                                                            >  1:1119238/1‑93 (MQ=255)

                gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATcc                                                                         >  2:3458018/1‑93 (MQ=255)

                gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATcc                                                                         <  1:4104513/93‑1 (MQ=255)

                gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATcc                                                                         <  2:2553202/93‑1 (MQ=255)

                gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATcc                                                                         <  2:5648444/93‑1 (MQ=255)

                 acTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACTAGGTAGGGCAGCGCCTGATGGGGGAATcaa                                                                        >  1:2070348/1‑91 (MQ=255)

                 acTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGGATCCa                                                                        >  2:1120445/1‑93 (MQ=255)

                 acTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCa                                                                        >  2:5688550/1‑93 (MQ=255)

                 acTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCa                                                                        >  1:1832829/1‑93 (MQ=255)

                 acTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCa                                                                        >  1:4208961/1‑93 (MQ=255)

                  cTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAg                                                                       >  2:3598200/1‑93 (MQ=255)

                   ttGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGc                                                                      >  1:1448344/1‑93 (MQ=255)

                   ttGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGc                                                                      >  2:5974959/1‑93 (MQ=255)

                     gTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGCATCCAGCgg                                                                    >  2:454028/1‑93 (MQ=255)

                      ttCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGc                                                                   >  1:2434799/1‑93 (MQ=255)

                      ttCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGc                                                                   >  1:6093852/1‑93 (MQ=255)

                        cATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCa                                                                 >  1:1013884/1‑93 (MQ=255)

                        cATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCa                                                                 <  1:1082583/93‑1 (MQ=255)

                        cATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCa                                                                 <  1:300573/93‑1 (MQ=255)

                         aTTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAg                                                                >  1:5033012/1‑93 (MQ=255)

                            gATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACt                                                             >  2:4364621/1‑93 (MQ=255)

                             aTGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACtt                                                            <  1:2443827/93‑1 (MQ=255)

                              tggtctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTa                                                           <  2:4729710/93‑1 (MQ=255)

                                gtctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATc                                                         <  2:2302425/93‑1 (MQ=255)

                                gtctggtcCATGTCTCTTCGCTGGACAATAACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATc                                                         >  2:5998410/1‑93 (MQ=255)

                                  ctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGc                                                       <  2:4224627/93‑1 (MQ=255)

                                  ctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGc                                                       >  1:133514/1‑93 (MQ=255)

                                    ggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCt                                                     >  1:3886575/1‑93 (MQ=255)

                                     gtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTg                                                    <  1:4798614/93‑1 (MQ=255)

                                      tcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTgg                                                   <  2:6260036/93‑1 (MQ=255)

                                      tcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTgg                                                   >  2:4868986/1‑93 (MQ=255)

                                        cATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGc                                                 >  2:2942512/1‑93 (MQ=255)

                                        cATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGc                                                 >  2:4382952/1‑93 (MQ=255)

                                            tctcTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATc                                             >  2:1837307/1‑93 (MQ=255)

                                            tctcTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATc                                             <  1:3720704/93‑1 (MQ=255)

                                              tctTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATcgc                                           >  1:1744905/1‑93 (MQ=255)

                                               ctTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATcgcg                                          >  2:4633236/1‑93 (MQ=255)

                                               ctTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATcgcg                                          <  2:1494436/93‑1 (MQ=255)

                                               ctTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATcgcg                                          <  1:2512412/93‑1 (MQ=255)

                                                ttCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGt                                         <  1:3474294/93‑1 (MQ=255)

                                                    cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGaa                                     >  2:493162/1‑93 (MQ=255)

                                                    cTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGaa                                     >  1:5170619/1‑93 (MQ=255)

                                                     tGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAg                                    >  1:336784/1‑93 (MQ=255)

                                                     tGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAg                                    >  2:2330024/1‑93 (MQ=255)

                                                       gACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGtt                                  <  2:5423905/93‑1 (MQ=255)

                                                       gACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGtt                                  <  1:3948477/93‑1 (MQ=255)

                                                       gACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGtt                                  <  1:4408273/93‑1 (MQ=255)

                                                          aaTGACTACTATCGCTTTGCCCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTcgc                               <  2:3373454/93‑1 (MQ=255)

                                                           aTGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGTGCGATCGCGTGGGAGTTcgcg                              >  2:1098271/1‑93 (MQ=255)

                                                           aTGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTcgcg                              >  1:2907031/1‑93 (MQ=255)

                                                           aTGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTcgcg                              >  1:5949884/1‑93 (MQ=255)

                                                            tGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGt                             >  1:3233327/1‑93 (MQ=255)

                                                              actactATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCg                           >  1:2251299/1‑93 (MQ=255)

                                                              actactATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCg                           >  2:4453648/1‑93 (MQ=255)

                                                              actactATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCg                           >  1:835566/1‑93 (MQ=255)

                                                               ctactaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGCTCGCGTCGa                          >  1:3280182/1‑93 (MQ=255)

                                                                tactaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGaa                         <  2:5766235/93‑1 (MQ=255)

                                                                tactaTCGCTTTGACCACGCAGGGCAGCGCCTTCTGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGCTCgcggcgga                         >  2:3124178/1‑91 (MQ=255)

                                                                 actaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAg                        >  2:3982437/1‑93 (MQ=255)

                                                                  ctaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGc                       >  1:5779545/1‑93 (MQ=255)

                                                                  ctaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGc                       <  1:3348116/93‑1 (MQ=255)

                                                                  ctaTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGc                       <  1:4472556/93‑1 (MQ=255)

                                                                    aTCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCgg                     >  2:5841412/1‑93 (MQ=255)

                                                                     tCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGt                    >  1:1936207/1‑93 (MQ=255)

                                                                      cGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGtt                   <  1:4842701/93‑1 (MQ=255)

                                                                       gCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGttc                  >  2:4477566/1‑92 (MQ=255)

                                                                       gCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTa                  >  1:801728/1‑93 (MQ=255)

                                                                        cTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTat                 >  1:3695729/1‑92 (MQ=255)

                                                                        cTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTaa                 >  2:1714835/1‑93 (MQ=255)

                                                                        cTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTaa                 >  2:1304015/1‑93 (MQ=255)

                                                                         tttGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCCGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAat                >  2:3747193/1‑93 (MQ=255)

                                                                         tttGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAat                >  2:2880305/1‑93 (MQ=255)

                                                                          ttGACCAGGGAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGGGATCGCGTGGAAGTTCGCGTCGAAGCGGGTAata               >  2:807441/1‑93 (MQ=255)

                                                                            gACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATg             >  2:2748913/1‑93 (MQ=255)

                                                                            gACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATg             <  1:5604104/93‑1 (MQ=255)

                                                                            gACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATg             >  1:1199459/1‑93 (MQ=255)

                                                                             aCCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATgg            >  1:2725433/1‑93 (MQ=255)

                                                                              ccAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGa           >  1:5507843/1‑93 (MQ=255)

                                                                                aGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACg         >  1:1884022/1‑93 (MQ=255)

                                                                                 ggTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa        >  1:520443/1‑93 (MQ=255)

                                                                                 ggTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa        >  1:234835/1‑93 (MQ=255)

                                                                                    aGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgcg     >  2:5054190/1‑93 (MQ=255)

                                                                                     gggCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATTGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgcgc    >  2:5527116/1‑93 (MQ=255)

                                                                                     gggCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgcgc    <  2:415591/93‑1 (MQ=255)

                                                                                       gCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCaa  >  2:6174387/1‑93 (MQ=255)

                                                                                       gCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCaa  <  2:3501662/93‑1 (MQ=255)

CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAA  >  NC_000913/4406208‑4406387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATC  >  NC_000913/4406205‑4406391

CTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAG                                                                                               >  SRR4302533.3179153/1‑94 (MQ=60)

  TCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCG                                                                                             <  SRR4302533.5456755/94‑1 (MQ=60)

   CTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGC                                                                                            >  SRR4302533.2153172/1‑94 (MQ=60)

     TCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCT                                                                                          <  SRR4302533.4869992/94‑1 (MQ=60)

      CGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTG                                                                                         >  SRR4302533.967378/1‑94 (MQ=60)

      CGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTG                                                                                         >  SRR4302533.6309182/1‑94 (MQ=60)

      CGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTG                                                                                         >  SRR4302533.1975115/1‑94 (MQ=60)

      CGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTG                                                                                         >  SRR4302533.1841633/1‑94 (MQ=60)

       GTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGA                                                                                        >  SRR4302533.6283623/1‑94 (MQ=60)

        TCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGAT                                                                                       >  SRR4302533.2490542/1‑94 (MQ=60)

        TCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGAT                                                                                       <  SRR4302533.2104544/94‑1 (MQ=60)

         CCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATG                                                                                      <  SRR4302533.5894193/94‑1 (MQ=60)

          CGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGG                                                                                     >  SRR4302533.2370244/1‑94 (MQ=60)

          CGGCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTGTCTTCGCTGGACATTGACTACTATCTCTTTGACTAGGGAGGGCAGCGCCTTATGG                                                                                     >  SRR4302533.6343949/1‑94 (MQ=60)

           GTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGG                                                                                    <  SRR4302533.822611/94‑1 (MQ=60)

           GTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGG                                                                                    >  SRR4302533.2532491/1‑94 (MQ=60)

           GTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGG                                                                                    <  SRR4302533.5951216/94‑1 (MQ=60)

            TCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGG                                                                                   <  SRR4302533.5234292/94‑1 (MQ=60)

            TCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGG                                                                                   <  SRR4302533.2722579/94‑1 (MQ=60)

            TCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGG                                                                                   <  SRR4302533.3722008/94‑1 (MQ=60)

              TGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGA                                                                                 >  SRR4302533.1881041/1‑94 (MQ=60)

               GGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAC                                                                                >  SRR4302533.5199562/1‑94 (MQ=60)

               GGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAA                                                                                >  SRR4302533.3573267/1‑94 (MQ=60)

               GGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAA                                                                                >  SRR4302533.1200475/1‑94 (MQ=60)

               GGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAA                                                                                >  SRR4302533.1125877/1‑94 (MQ=60)

                GACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAAT                                                                               <  SRR4302533.350253/94‑1 (MQ=60)

                GACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAAT                                                                               <  SRR4302533.5903754/94‑1 (MQ=60)

                   GACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACTAGGTAGGGCAGCGCCTGATGGGGGAATCAA                                                                            >  SRR4302533.2083099/1‑94 (MQ=60)

                   GACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCA                                                                            <  SRR4302533.4129478/94‑1 (MQ=60)

                   GACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCA                                                                            >  SRR4302533.1844015/1‑94 (MQ=60)

                   GACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCA                                                                            >  SRR4302533.2214495/1‑94 (MQ=60)

                   GACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCA                                                                            >  SRR4302533.4234423/1‑94 (MQ=60)

                    ACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAG                                                                           >  SRR4302533.2742092/1‑94 (MQ=60)

                     CTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGC                                                                          >  SRR4302533.98007/1‑94 (MQ=60)

                     CTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGC                                                                          >  SRR4302533.1027122/1‑94 (MQ=60)

                     CTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGC                                                                          >  SRR4302533.1457082/1‑94 (MQ=60)

                       TGTTCATTGATGGTCTGGTCCATGTCTCTTCGCGGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCTGCGG                                                                        <  SRR4302533.854207/94‑1 (MQ=60)

                        GTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGC                                                                       >  SRR4302533.2449837/1‑94 (MQ=60)

                        GTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGC                                                                       >  SRR4302533.6130672/1‑94 (MQ=60)

                          TCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCA                                                                     >  SRR4302533.1019820/1‑94 (MQ=60)

                           CATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAG                                                                    >  SRR4302533.5063501/1‑94 (MQ=60)

                           CATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAG                                                                    <  SRR4302533.3878672/94‑1 (MQ=60)

                           CATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAG                                                                    <  SRR4302533.1088969/94‑1 (MQ=60)

                           CATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAG                                                                    <  SRR4302533.302412/94‑1 (MQ=60)

                            ATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCCGA                                                                   >  SRR4302533.1124644/1‑94 (MQ=60)

                              TGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACT                                                                 >  SRR4302533.2458176/1‑94 (MQ=60)

                                ATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTA                                                               >  SRR4302533.5717336/1‑94 (MQ=60)

                                ATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTA                                                               <  SRR4302533.2458911/94‑1 (MQ=60)

                                 TGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTAT                                                              <  SRR4302533.6223193/94‑1 (MQ=60)

                                   GTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCG                                                            <  SRR4302533.3413200/94‑1 (MQ=60)

                                   GTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCG                                                            <  SRR4302533.3384592/94‑1 (MQ=60)

                                    TCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGC                                                           >  SRR4302533.134355/1‑94 (MQ=60)

                                      TGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCT                                                         <  SRR4302533.3095638/94‑1 (MQ=60)

                                      TGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCT                                                         >  SRR4302533.3910285/1‑94 (MQ=60)

                                      TGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCT                                                         >  SRR4302533.4211607/1‑94 (MQ=60)

                                       TGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTG                                                        <  SRR4302533.363344/94‑1 (MQ=60)

                                       GGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTG                                                        <  SRR4302533.2921436/94‑1 (MQ=60)

                                       GGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTG                                                        >  SRR4302533.2461683/1‑94 (MQ=60)

                                        GTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGG                                                       >  SRR4302533.1755443/1‑94 (MQ=60)

                                        GTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGG                                                       <  SRR4302533.4827681/94‑1 (MQ=60)

                                            ATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGA                                                   >  SRR4302533.4113070/1‑94 (MQ=60)

                                             TGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGAT                                                  <  SRR4302533.3812456/94‑1 (MQ=60)

                                             TGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGGAGGGCAGCGCCTGCTGGGGGAATCCAGCGGCCAGAGTTATCGCCTGGGCGAT                                                  >  SRR4302533.1946888/1‑94 (MQ=60)

                                               TCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCG                                                >  SRR4302533.1866308/1‑94 (MQ=60)

                                               TCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCG                                                <  SRR4302533.3743538/94‑1 (MQ=60)

                                               TCTCTGCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCG                                                <  SRR4302533.1653619/94‑1 (MQ=60)

                                                CTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGC                                               >  SRR4302533.1755517/1‑94 (MQ=60)

                                                CTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGC                                               >  SRR4302533.3066236/1‑94 (MQ=60)

                                                 TCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCG                                              >  SRR4302533.960138/1‑94 (MQ=60)

                                                  CTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGT                                             <  SRR4302533.2527890/94‑1 (MQ=60)

                                                   TTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTG                                            <  SRR4302533.3495731/94‑1 (MQ=60)

                                                    TCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGG                                           <  SRR4302533.3486560/94‑1 (MQ=60)

                                                      GCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAA                                         >  SRR4302533.5201824/1‑94 (MQ=60)

                                                      GCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAA                                         <  SRR4302533.4356607/94‑1 (MQ=60)

                                                       CTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        >  SRR4302533.338841/1‑94 (MQ=60)

                                                       CTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        <  SRR4302533.3927586/94‑1 (MQ=60)

                                                       CTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAG                                        >  SRR4302533.4466471/1‑94 (MQ=60)

                                                        TGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGT                                       <  SRR4302533.3338531/94‑1 (MQ=60)

                                                          GACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTC                                     <  SRR4302533.4434895/94‑1 (MQ=60)

                                                          GACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTC                                     <  SRR4302533.3972555/94‑1 (MQ=60)

                                                          GACAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTC                                     <  SRR4302533.3972556/94‑1 (MQ=60)

                                                            CAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGC                                   >  SRR4302533.1363357/1‑94 (MQ=60)

                                                            CAATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGAGTGGAAGTTCGG                                   >  SRR4302533.5389340/1‑94 (MQ=60)

                                                             AATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCG                                  >  SRR4302533.5985868/1‑94 (MQ=60)

                                                             AATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCG                                  >  SRR4302533.2924879/1‑94 (MQ=60)

                                                              ATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGT                                 >  SRR4302533.6249534/1‑94 (MQ=60)

                                                              ATGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGT                                 >  SRR4302533.3253251/1‑94 (MQ=60)

                                                               TGACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTC                                >  SRR4302533.5572553/1‑94 (MQ=60)

                                                                GACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCG                               <  SRR4302533.295580/94‑1 (MQ=60)

                                                                GACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCG                               >  SRR4302533.840463/1‑94 (MQ=60)

                                                                GACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCG                               >  SRR4302533.2265216/1‑94 (MQ=60)

                                                                 ACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGA                              >  SRR4302533.1311706/1‑94 (MQ=60)

                                                                 ACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGA                              >  SRR4302533.2441803/1‑94 (MQ=60)

                                                                 ACTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGCTCGCGTCGA                              >  SRR4302533.3300433/1‑94 (MQ=60)

                                                                  CTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAA                             >  SRR4302533.4442097/1‑94 (MQ=60)

                                                                  CTACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAA                             >  SRR4302533.1994312/1‑94 (MQ=60)

                                                                   TACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAG                            <  SRR4302533.3289600/94‑1 (MQ=60)

                                                                   TACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAG                            >  SRR4302533.4696302/1‑94 (MQ=60)

                                                                    ACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGC                           >  SRR4302533.5814450/1‑94 (MQ=60)

                                                                    ACTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGC                           >  SRR4302533.3324746/1‑94 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCG                          <  SRR4302533.4499608/94‑1 (MQ=60)

                                                                     CTATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCG                          <  SRR4302533.3368800/94‑1 (MQ=60)

                                                                      TATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGG                         <  SRR4302533.5033889/94‑1 (MQ=60)

                                                                       ATCGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGT                        >  SRR4302533.1948017/1‑94 (MQ=60)

                                                                         CGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTA                      <  SRR4302533.4872052/94‑1 (MQ=60)

                                                                         CGCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTA                      >  SRR4302533.806441/1‑94 (MQ=60)

                                                                          GCTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAT                     >  SRR4302533.3718430/1‑94 (MQ=60)

                                                                           CTTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAAT                    <  SRR4302533.4862254/94‑1 (MQ=60)

                                                                            TTTGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATA                   <  SRR4302533.2797593/94‑1 (MQ=60)

                                                                              TGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATG                 >  SRR4302533.1206663/1‑94 (MQ=60)

                                                                              TGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATG                 <  SRR4302533.6118232/94‑1 (MQ=60)

                                                                              TGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATG                 <  SRR4302533.3605486/94‑1 (MQ=60)

                                                                              TGACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATG                 <  SRR4302533.2277097/94‑1 (MQ=60)

                                                                               GACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGG                >  SRR4302533.2742153/1‑94 (MQ=60)

                                                                               GACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGG                >  SRR4302533.4326222/1‑94 (MQ=60)

                                                                               GACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGG                <  SRR4302533.5637962/94‑1 (MQ=60)

                                                                                ACCAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGA               >  SRR4302533.5541086/1‑94 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             >  SRR4302533.1895528/1‑94 (MQ=60)

                                                                                  CAGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACG             >  SRR4302533.2574710/1‑94 (MQ=60)

                                                                                   AGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGA            >  SRR4302533.1111494/1‑94 (MQ=60)

                                                                                   AGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGA            >  SRR4302533.523531/1‑94 (MQ=60)

                                                                                   AGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGA            >  SRR4302533.236283/1‑94 (MQ=60)

                                                                                   AGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGA            >  SRR4302533.2193500/1‑94 (MQ=60)

                                                                                   AGGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGA            <  SRR4302533.5101226/94‑1 (MQ=60)

                                                                                    GGTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAG           <  SRR4302533.5680115/94‑1 (MQ=60)

                                                                                     GTAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGC          <  SRR4302533.5310949/94‑1 (MQ=60)

                                                                                      TAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCG         <  SRR4302533.648946/94‑1 (MQ=60)

                                                                                      TAGGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCG         <  SRR4302533.5869499/94‑1 (MQ=60)

                                                                                        GGGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCA       >  SRR4302533.753779/1‑94 (MQ=60)

                                                                                         GGCAGCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAA      <  SRR4302533.1236400/94‑1 (MQ=60)

                                                                                             GCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATC  >  SRR4302533.5177128/1‑94 (MQ=60)

CTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATC  >  NC_000913/4406205‑4406391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`