Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,428,092 A→G G217G (GGA→GGG)  cycA → transport of D‑alanine, D‑serine, and glycine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,428,0920AG100.0% 650.5 / NA 200G217G (GGA→GGG) cycAtransport of D‑alanine, D‑serine, and glycine
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (99/101);  total (99/101)

Only 100 of 209 total aligned reads displayed.

AGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACG  >  NC_000913/4428004‑4428181
                                                                                        |                                                                                         
aGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTcc                                                                                       >  2:1150914/1‑93 (MQ=255)
 gTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCa                                                                                      <  1:3891431/93‑1 (MQ=255)
 gTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCa                                                                                      <  2:3593140/93‑1 (MQ=255)
 gTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCa                                                                                      >  1:1527219/1‑93 (MQ=255)
  tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGGCGGGTTGCAg                                                                                     >  1:1158376/1‑93 (MQ=255)
  tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAg                                                                                     <  2:2892398/93‑1 (MQ=255)
    aCCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGAt                                                                                   <  1:5227670/93‑1 (MQ=255)
      cGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAg                                                                                 <  2:2666108/93‑1 (MQ=255)
        aCTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCg                                                                               >  1:2617510/1‑93 (MQ=255)
         cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCgg                                                                              <  1:2262433/93‑1 (MQ=255)
         cTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCgg                                                                              >  1:318994/1‑93 (MQ=255)
          tGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGt                                                                             >  2:1224777/1‑93 (MQ=255)
          tGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGt                                                                             >  2:2223845/1‑93 (MQ=255)
           ggTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGtt                                                                            <  1:6274127/93‑1 (MQ=255)
           ggTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGtt                                                                            <  1:2925723/93‑1 (MQ=255)
             tgtgGAAGCGTCATTCGCGCATGTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGtttt                                                                          <  1:3278745/93‑1 (MQ=255)
               tgGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCg                                                                        <  2:3448856/93‑1 (MQ=255)
                ggAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGc                                                                       >  1:3360587/1‑93 (MQ=255)
                ggAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGc                                                                       <  2:1810262/93‑1 (MQ=255)
                 gAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCt                                                                      >  2:5829275/1‑93 (MQ=255)
                 gAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCt                                                                      <  1:2975125/93‑1 (MQ=255)
                     cGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCg                                                                  <  1:4364740/93‑1 (MQ=255)
                      gTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGt                                                                 >  1:808083/1‑93 (MQ=255)
                       tCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTg                                                                <  1:1349272/93‑1 (MQ=255)
                       tCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTg                                                                >  2:5239156/1‑93 (MQ=255)
                           tCGCGCTTTTGTGGAATGACGGTGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGa                                                            <  2:5146550/93‑1 (MQ=255)
                            cgcgCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGAt                                                           <  1:5602865/93‑1 (MQ=255)
                             gcgcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGAtt                                                          <  1:6058194/93‑1 (MQ=255)
                             gcgcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGAtt                                                          >  1:3426062/1‑93 (MQ=255)
                              cgcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTg                                                         >  1:1138680/1‑93 (MQ=255)
                               gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                        <  1:3556449/93‑1 (MQ=255)
                               gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                        <  2:2112487/93‑1 (MQ=255)
                               gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                        <  1:1416868/93‑1 (MQ=255)
                               gcATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGa                                                        >  1:517300/1‑93 (MQ=255)
                                cATTTGTGGAATGACGGCGGCTGGTTCCCGATAGGTTTAAGTGGCTTCTTTGTCGGGGTCCAGATAGCGGTTTTCGCTTTCGTGGGGAGTggg                                                       >  1:2485495/1‑91 (MQ=255)
                                cATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAg                                                       <  2:1073881/93‑1 (MQ=255)
                                cATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAg                                                       >  1:241165/1‑93 (MQ=255)
                                 aTTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGATTAAGTGGCTTCTTTGCCGGGATCCAGATAGCGGTTTTCGCTCTCGTGGGGACTGAGc                                                      >  2:3373179/1‑93 (MQ=255)
                                  tttGTGGAATGACGGCGGCTGGTTGCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     <  1:5197972/93‑1 (MQ=255)
                                  tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     >  2:785033/1‑93 (MQ=255)
                                  tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     >  1:5086651/1‑93 (MQ=255)
                                  tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     <  1:21500/93‑1 (MQ=255)
                                  tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     <  1:2169998/93‑1 (MQ=255)
                                  tttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCt                                                     >  1:4500939/1‑93 (MQ=255)
                                   ttGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTg                                                    <  1:4830336/93‑1 (MQ=255)
                                    tgtgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTgg                                                   <  1:1362381/93‑1 (MQ=255)
                                     gtgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggt                                                  >  1:3017635/1‑93 (MQ=255)
                                     gtgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggt                                                  <  2:1991582/93‑1 (MQ=255)
                                      tgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggta                                                 <  2:3120440/93‑1 (MQ=255)
                                      tgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggta                                                 <  2:5278785/93‑1 (MQ=255)
                                      tgGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTGCGTGGGGATTGAGCTggta                                                 <  2:6104949/93‑1 (MQ=255)
                                       ggAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtag                                                <  1:1891302/93‑1 (MQ=255)
                                          aTGGCGGCGTCTGGTGCCCGAAAGGTGTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTggtaggta                                             <  1:3079210/93‑1 (MQ=255)
                                             accgcggcTTGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaa                                          <  2:6185334/90‑1 (MQ=255)
                                             aCGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaa                                          <  1:3956118/93‑1 (MQ=255)
                                               ggcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTacaaca                                        >  1:4885324/1‑93 (MQ=255)
                                                gcggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAg                                       >  2:1601357/1‑93 (MQ=255)
                                                 cggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGc                                      <  2:4657682/93‑1 (MQ=255)
                                                 cggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGc                                      <  2:555700/93‑1 (MQ=255)
                                                  ggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCt                                     >  1:710979/1‑93 (MQ=255)
                                                  ggcTGGTTCCCGAAAGGGTTTAGTGGCTTCTTTTTCGGGGGGCCGATAACGGGTTTTGCGTTTGTGGGGGGTTAGGTGGGGGGTtcaacaacc                                     >  2:4234879/1‑90 (MQ=255)
                                                   gcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTg                                    >  2:3965785/1‑93 (MQ=255)
                                                   gcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGGTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGGACAACAGCTg                                    >  2:2417295/1‑93 (MQ=255)
                                                    cTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGc                                   <  1:3296673/93‑1 (MQ=255)
                                                    cTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGc                                   <  2:5319511/93‑1 (MQ=255)
                                                     tGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGTGCTGGTAGGTACAACAGCTGCg                                  >  2:2764890/1‑93 (MQ=255)
                                                     tGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCg                                  >  2:507636/1‑93 (MQ=255)
                                                     tGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCg                                  >  1:992232/1‑93 (MQ=255)
                                                      ggTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCgg                                 <  1:1488264/93‑1 (MQ=255)
                                                        ttCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGaa                               <  1:5312952/93‑1 (MQ=255)
                                                         tCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGaaa                              >  1:276765/1‑93 (MQ=255)
                                                          cccGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAc                             <  2:4989378/93‑1 (MQ=255)
                                                            cGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCa                           >  2:5654324/1‑93 (MQ=255)
                                                            cGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCa                           >  2:3483551/1‑93 (MQ=255)
                                                             gAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaa                          >  2:4882640/1‑93 (MQ=255)
                                                              aaaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaaa                         <  2:4644033/93‑1 (MQ=255)
                                                              aaaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCaaa                         <  2:4266996/93‑1 (MQ=255)
                                                               aaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAg                        <  2:372133/93‑1 (MQ=255)
                                                               aaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAg                        >  1:5835503/1‑93 (MQ=255)
                                                               aaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAg                        >  1:6068368/1‑93 (MQ=255)
                                                               aaGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAg                        >  1:866956/1‑93 (MQ=255)
                                                                aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                       >  2:4716534/1‑93 (MQ=255)
                                                                aGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGa                       <  2:3021831/93‑1 (MQ=255)
                                                                 ggTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAt                      <  2:4087033/93‑1 (MQ=255)
                                                                 ggTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAt                      >  2:1509026/1‑93 (MQ=255)
                                                                  gTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATc                     <  2:684407/93‑1 (MQ=255)
                                                                    ttAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGCTTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCa                   >  2:3280041/1‑93 (MQ=255)
                                                                    ttAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCa                   >  1:1771169/1‑93 (MQ=255)
                                                                     tAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCag                  >  1:1016326/1‑93 (MQ=255)
                                                                     tAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCag                  >  1:4861120/1‑93 (MQ=255)
                                                                       aGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCagag                <  1:3950099/93‑1 (MQ=255)
                                                                            cttcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATc           >  1:1597986/1‑93 (MQ=255)
                                                                              tcttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAc         <  1:1650345/93‑1 (MQ=255)
                                                                               cttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACt        >  1:53609/1‑93 (MQ=255)
                                                                               cttTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACt        <  1:4698584/93‑1 (MQ=255)
                                                                                tttGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTg       >  2:2459891/1‑93 (MQ=255)
                                                                                 ttGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGc      >  1:599631/1‑93 (MQ=255)
                                                                                  tGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGcc     <  2:712455/93‑1 (MQ=255)
                                                                                   gCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCa    >  1:4200567/1‑93 (MQ=255)
                                                                                     cGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCAcg  >  1:5975957/1‑93 (MQ=255)
                                                                                        |                                                                                         
AGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACG  >  NC_000913/4428004‑4428181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  >  NC_000913/4427999‑4428183
                                                                                             |                                                                                           
CTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGG                                                                                             <  SRR4302533.4110084/94‑1 (MQ=60)
 TTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGT                                                                                            >  SRR4302533.2844796/1‑94 (MQ=60)
     AGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCA                                                                                        <  SRR4302533.841294/94‑1 (MQ=60)
     AGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCA                                                                                        >  SRR4302533.1536412/1‑94 (MQ=60)
      GTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGGCGGGTTGCAG                                                                                       >  SRR4302533.1165328/1‑94 (MQ=60)
      GTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAG                                                                                       <  SRR4302533.3915176/94‑1 (MQ=60)
       TCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGA                                                                                      >  SRR4302533.4511735/1‑94 (MQ=60)
        CACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGGTTAAGTGGCTTCTTTGCCGGGTTCCAGAT                                                                                     >  SRR4302533.698468/1‑94 (MQ=60)
         ACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATA                                                                                    <  SRR4302533.5259123/94‑1 (MQ=60)
         ACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATA                                                                                    <  SRR4302533.5246997/94‑1 (MQ=60)
           CGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGC                                                                                  >  SRR4302533.1404885/1‑94 (MQ=60)
           CGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGGTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGC                                                                                  >  SRR4302533.1809061/1‑94 (MQ=60)
            GACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCG                                                                                 >  SRR4302533.2633575/1‑94 (MQ=60)
            GACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCG                                                                                 <  SRR4302533.2462530/94‑1 (MQ=60)
             ACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGG                                                                                >  SRR4302533.320949/1‑94 (MQ=60)
             ACTGGTGTGGAAGCGTCATTCGCGCATGTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGG                                                                                <  SRR4302533.1441713/94‑1 (MQ=60)
              CTTGTATGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGATCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGT                                                                               <  SRR4302533.2195267/94‑1 (MQ=60)
              CTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGT                                                                               <  SRR4302533.2276427/94‑1 (MQ=60)
              CTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGT                                                                               >  SRR4302533.2006225/1‑94 (MQ=60)
                GGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTT                                                                             <  SRR4302533.6311600/94‑1 (MQ=60)
                GGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTT                                                                             <  SRR4302533.2943626/94‑1 (MQ=60)
                GGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTT                                                                             >  SRR4302533.1704577/1‑94 (MQ=60)
                  TGTGGAAGCGTCATTCGCGCATGTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTC                                                                           <  SRR4302533.3298989/94‑1 (MQ=60)
                   GTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCG                                                                          <  SRR4302533.1559506/94‑1 (MQ=60)
                    TGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGC                                                                         >  SRR4302533.3381349/1‑94 (MQ=60)
                      GAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTT                                                                       <  SRR4302533.2993329/94‑1 (MQ=60)
                       AAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGGTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGGTTTCGCTTT                                                                      >  SRR4302533.3151757/1‑94 (MQ=60)
                          CGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGT                                                                   >  SRR4302533.2871311/1‑94 (MQ=60)
                          CGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGT                                                                   <  SRR4302533.4391091/94‑1 (MQ=60)
                          CGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGT                                                                   >  SRR4302533.812832/1‑94 (MQ=60)
                            TCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGG                                                                 <  SRR4302533.1357389/94‑1 (MQ=60)
                                 CGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATT                                                            >  SRR4302533.4867580/1‑94 (MQ=60)
                                 CGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATT                                                            <  SRR4302533.5636720/94‑1 (MQ=60)
                                 CGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATT                                                            >  SRR4302533.3447226/1‑94 (MQ=60)
                                 CGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATT                                                            <  SRR4302533.3217448/94‑1 (MQ=60)
                                 CGCGCATTGGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATT                                                            <  SRR4302533.489548/94‑1 (MQ=60)
                                  GCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTG                                                           <  SRR4302533.6094873/94‑1 (MQ=60)
                                  GCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTG                                                           >  SRR4302533.1145456/1‑94 (MQ=60)
                                   CGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGA                                                          >  SRR4302533.520372/1‑94 (MQ=60)
                                    GCATTTGTGGAATGACGGCGGCTGGTTCCCGATAGGTTTAAGTGGCTTCTTTGTCGGGGTCCAGATAGCGGTTTTCGCTTTCGTGGGGAgtggg                                                         >  SRR4302533.2500810/1‑89 (MQ=60)
                                    GCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAG                                                         <  SRR4302533.3578368/94‑1 (MQ=60)
                                    GCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAG                                                         >  SRR4302533.242662/1‑94 (MQ=60)
                                    GCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAG                                                         <  SRR4302533.1370107/94‑1 (MQ=60)
                                    GCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAG                                                         <  SRR4302533.1425440/94‑1 (MQ=60)
                                      ATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCT                                                       >  SRR4302533.395634/1‑94 (MQ=60)
                                      ATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCT                                                       >  SRR4302533.4528202/1‑94 (MQ=60)
                                      ATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCT                                                       >  SRR4302533.5117387/1‑94 (MQ=60)
                                       TTTGTGGAATGACGGCGGCTGGTTGCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTG                                                      <  SRR4302533.5229319/94‑1 (MQ=60)
                                       TTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTG                                                      <  SRR4302533.2183342/94‑1 (MQ=60)
                                       TTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTG                                                      <  SRR4302533.21642/94‑1 (MQ=60)
                                        TTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGG                                                     <  SRR4302533.4859613/94‑1 (MQ=60)
                                         TGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGT                                                    >  SRR4302533.3036090/1‑94 (MQ=60)
                                         TGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGT                                                    <  SRR4302533.1370563/94‑1 (MQ=60)
                                          GTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTA                                                   <  SRR4302533.1912378/94‑1 (MQ=60)
                                            GGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGG                                                 <  SRR4302533.1902847/94‑1 (MQ=60)
                                            GGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGG                                                 >  SRR4302533.6212487/1‑94 (MQ=60)
                                             GAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGT                                                <  SRR4302533.5225858/94‑1 (MQ=60)
                                               ATGGCGGCGTCTGGTGCCCGAAAGGTGTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTAC                                              <  SRR4302533.3098107/94‑1 (MQ=60)
                                                TGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACA                                             <  SRR4302533.5789642/94‑1 (MQ=60)
                                                  ACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAAC                                           <  SRR4302533.5901880/94‑1 (MQ=60)
                                                  ACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAAC                                           <  SRR4302533.3980234/94‑1 (MQ=60)
                                                   CGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACA                                          <  SRR4302533.3166801/94‑1 (MQ=60)
                                                   CGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACA                                          >  SRR4302533.4914920/1‑94 (MQ=60)
                                                    GGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAG                                         <  SRR4302533.397563/94‑1 (MQ=60)
                                                      CGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCT                                       >  SRR4302533.715178/1‑94 (MQ=60)
                                                       GGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTG                                      >  SRR4302533.4832098/1‑94 (MQ=60)
                                                         CTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCG                                    >  SRR4302533.998033/1‑94 (MQ=60)
                                                         CTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCG                                    <  SRR4302533.3317019/94‑1 (MQ=60)
                                                          TGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGG                                   <  SRR4302533.5074799/94‑1 (MQ=60)
                                                           GGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGA                                  <  SRR4302533.1497242/94‑1 (MQ=60)
                                                             TTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAA                                <  SRR4302533.5344791/94‑1 (MQ=60)
                                                             TTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAA                                <  SRR4302533.1094148/94‑1 (MQ=60)
                                                             TTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAA                                >  SRR4302533.278450/1‑94 (MQ=60)
                                                              TCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAC                               <  SRR4302533.6031395/94‑1 (MQ=60)
                                                              TCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAAC                               >  SRR4302533.6093465/1‑94 (MQ=60)
                                                               CCCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACC                              >  SRR4302533.5259994/1‑94 (MQ=60)
                                                                CCGAAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCA                             >  SRR4302533.3309676/1‑94 (MQ=60)
                                                                  GAAAGGTTTAAGTGGCTCCTTTGCCGGGTTCCAGATAGCGGTTATCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAA                           >  SRR4302533.4473756/1‑94 (MQ=60)
                                                                   AAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAG                          >  SRR4302533.5870765/1‑94 (MQ=60)
                                                                   AAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAG                          >  SRR4302533.872037/1‑94 (MQ=60)
                                                                   AAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAG                          >  SRR4302533.6105086/1‑94 (MQ=60)
                                                                   AAAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAG                          >  SRR4302533.2724215/1‑94 (MQ=60)
                                                                    AAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGA                         >  SRR4302533.2289583/1‑94 (MQ=60)
                                                                    AAGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGA                         <  SRR4302533.4545088/94‑1 (MQ=60)
                                                                     AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        <  SRR4302533.5398107/94‑1 (MQ=60)
                                                                     AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        >  SRR4302533.1635688/1‑94 (MQ=60)
                                                                     AGGTTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGAT                        <  SRR4302533.297099/94‑1 (MQ=60)
                                                                        TTTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCA                     >  SRR4302533.1781951/1‑94 (MQ=60)
                                                                         TTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGGTTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAG                    <  SRR4302533.2191799/94‑1 (MQ=60)
                                                                         TTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAG                    >  SRR4302533.4890581/1‑94 (MQ=60)
                                                                         TTAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAG                    >  SRR4302533.1022300/1‑94 (MQ=60)
                                                                          TAAGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGA                   >  SRR4302533.5598886/1‑94 (MQ=60)
                                                                            AGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGA                 <  SRR4302533.811606/94‑1 (MQ=60)
                                                                            AGTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGA                 <  SRR4302533.3974184/94‑1 (MQ=60)
                                                                             GTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAA                <  SRR4302533.515726/94‑1 (MQ=60)
                                                                             GTGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAA                <  SRR4302533.6101202/94‑1 (MQ=60)
                                                                              TGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAA               >  SRR4302533.5370960/1‑94 (MQ=60)
                                                                              TGGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATACAGAGAAA               >  SRR4302533.4346698/1‑94 (MQ=60)
                                                                               GGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGTGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAT              >  SRR4302533.3392214/1‑94 (MQ=60)
                                                                               GGCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAAT              >  SRR4302533.3469297/1‑94 (MQ=60)
                                                                                GCTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATC             >  SRR4302533.1607660/1‑94 (MQ=60)
                                                                                 CTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCA            <  SRR4302533.3597115/94‑1 (MQ=60)
                                                                                 CTTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCA            <  SRR4302533.123073/94‑1 (MQ=60)
                                                                                  TTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAC           >  SRR4302533.1525790/1‑94 (MQ=60)
                                                                                  TTCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCAC           >  SRR4302533.1154695/1‑94 (MQ=60)
                                                                                   TCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACT          <  SRR4302533.1660344/94‑1 (MQ=60)
                                                                                   TCTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACT          >  SRR4302533.53972/1‑94 (MQ=60)
                                                                                    CTTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTG         <  SRR4302533.4727063/94‑1 (MQ=60)
                                                                                     TTTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGC        >  SRR4302533.603159/1‑94 (MQ=60)
                                                                                      TTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCC       <  SRR4302533.959053/94‑1 (MQ=60)
                                                                                      TTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCC       <  SRR4302533.3048185/94‑1 (MQ=60)
                                                                                      TTGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCC       <  SRR4302533.3010979/94‑1 (MQ=60)
                                                                                       TGCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCA      >  SRR4302533.4225986/1‑94 (MQ=60)
                                                                                        GCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCAC     <  SRR4302533.414421/94‑1 (MQ=60)
                                                                                        GCCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCAC     >  SRR4302533.3323795/1‑94 (MQ=60)
                                                                                         CCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACG    >  SRR4302533.6012135/1‑94 (MQ=60)
                                                                                         CCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACG    <  SRR4302533.261338/94‑1 (MQ=60)
                                                                                         CCGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACG    >  SRR4302533.2172694/1‑94 (MQ=60)
                                                                                          CGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGC   >  SRR4302533.5387141/1‑94 (MQ=60)
                                                                                          CGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGC   >  SRR4302533.5927108/1‑94 (MQ=60)
                                                                                          CGGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGC   >  SRR4302533.4074753/1‑94 (MQ=60)
                                                                                           GGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  <  SRR4302533.3212429/94‑1 (MQ=60)
                                                                                           GGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  >  SRR4302533.3430611/1‑94 (MQ=60)
                                                                                           GGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  <  SRR4302533.5745631/94‑1 (MQ=60)
                                                                                           GGGTTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  <  SRR4302533.1023636/94‑1 (MQ=60)
                                                                                             |                                                                                           
CTTTCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTACAACAGCTGCGGAAACCAAAGATCCAGAGAAATCACTGCCACGCG  >  NC_000913/4427999‑4428183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑